CN114616343A - 用于在甲基化分区测定中分析无细胞dna的组合物和方法 - Google Patents

用于在甲基化分区测定中分析无细胞dna的组合物和方法 Download PDF

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CN114616343A
CN114616343A CN202080076649.5A CN202080076649A CN114616343A CN 114616343 A CN114616343 A CN 114616343A CN 202080076649 A CN202080076649 A CN 202080076649A CN 114616343 A CN114616343 A CN 114616343A
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nucleobase
dna
subsample
cfdna
fold
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斯特凡尼·安·沃德·莫蒂默
威廉·J·格林利夫
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Guardant Health Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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CN202080076649.5A 2019-09-30 2020-09-30 用于在甲基化分区测定中分析无细胞dna的组合物和方法 Pending CN114616343A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962908569P 2019-09-30 2019-09-30
US62/908,569 2019-09-30
PCT/US2020/053610 WO2021067484A1 (en) 2019-09-30 2020-09-30 Compositions and methods for analyzing cell-free dna in methylation partitioning assays

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CN114616343A true CN114616343A (zh) 2022-06-10

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US (2) US11891653B2 (https=)
EP (1) EP4041919A1 (https=)
JP (2) JP7717057B2 (https=)
CN (1) CN114616343A (https=)
CA (1) CA3151538A1 (https=)
WO (1) WO2021067484A1 (https=)

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WO2025038399A1 (en) * 2023-08-11 2025-02-20 Guardant Health, Inc. Methylated enrichment methods for single-molecule genetic and epigenetic sequencing
WO2025155895A1 (en) * 2024-01-19 2025-07-24 Guardant Health, Inc. Nucleic acid modification profiling method
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US20210115502A1 (en) 2021-04-22
JP2022550131A (ja) 2022-11-30
EP4041919A1 (en) 2022-08-17
JP2025148581A (ja) 2025-10-07
JP7717057B2 (ja) 2025-08-01
WO2021067484A1 (en) 2021-04-08
CA3151538A1 (en) 2021-04-08
US20240229112A1 (en) 2024-07-11
US11891653B2 (en) 2024-02-06

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