CN114601039A - Method for improving oestrus rate and conception rate of sheep under heat stress - Google Patents
Method for improving oestrus rate and conception rate of sheep under heat stress Download PDFInfo
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- CN114601039A CN114601039A CN202210259635.8A CN202210259635A CN114601039A CN 114601039 A CN114601039 A CN 114601039A CN 202210259635 A CN202210259635 A CN 202210259635A CN 114601039 A CN114601039 A CN 114601039A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
Abstract
The invention discloses a method for improving the oestrus rate and the conception rate of sheep under heat stress. Adding 0.05-0.2% of cholesterol substances into sheep feed or water, and feeding sheep ewes from before estrus to 30 days after hybridization in high-temperature seasons. According to the invention, 0.2g-1.0 g/one day of cholesterol is added into the ewe feed or drinking water in the period from the high-temperature season before estrus to 30 days after hybridization, so that the influence of heat stress on the estrus rate and the hybridization rate of sheep can be obviously relieved, and the economic benefit of a farm is improved.
Description
Technical Field
The invention belongs to the field of animal breeding, and relates to a method for improving the oestrus rate and the conception rate of sheep under heat stress.
Background
With the intensive development of sheep breeding and the aggravation of global warming, the adverse effect of heat stress on the reproductive performance of sheep becomes increasingly serious. Heat stress affects sheep oocyte later stage development (Ahmadi E et al; 2019), embryonic development and implantation, pregnancy maintenance, fetal development, as evidenced by prolonged estrous cycle, decreased conception rate and fetal survival (Gharibzadeh Z et al, 2015; Macaas-Cruz U et al, 2016; Romo-Barron CB et al, 2019). Meta analysis of 48 experiments of 36 studies by Romo-Barron CB et al (2019) showed that: heat stress makes the oestrus expression of the ewe unobvious, the oestrus duration is shortened by 7.09 hours, the oestrus cycle is prolonged by 0.57 day, the pregnancy rate is reduced by 58.8 percent, the embryo death rate is 12.36 times of that of a normal control group, in addition, the placenta weight is obviously reduced (-183.62g), the lamb birth weight is reduced by 0.99kg, and the difference with the control group reaches an obvious level (P is less than 0.05). Generally, sheep are suitably at a temperature of 21 ℃ to 25 ℃ and if the ambient temperature is too high, heat stress will occur. With the gradual rise of global temperature and the intensive large-scale development of sheep raising industry, the heat stress of sheep flocks is more and more serious, and huge economic loss is brought to the breeding industry.
Cholesterol is an important component constituting cell membranes and accounts for over 20% of plasma membrane lipids. Cholesterol can prevent the disorder of the plasma membrane bilayer at high temperature, and can prevent the formation of crystal at low temperature, so as to maintain the fluidity and the stability of the structure of the plasma membrane. Meanwhile, cholesterol is a precursor substance for synthesis of steroid hormones (sex hormone and adrenocortical hormone), and the imbalance of cholesterol homeostasis can also influence the synthesis of progesterone and estrogen by influencing the expression of an ovary luteinizing hormone receptor. However, no report has been made on the influence of heat stress on the oestrus rate and conception rate of sheep by cholesterol supplementation.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides application of cholesterol in preparing an additive or feed for relieving the influence of heat stress on the oestrus rate and the conception rate of sheep.
It is another object of the present invention to provide a method for increasing the oestrus and conception rates of sheep under heat stress.
It is a further object of the present invention to provide a sheep feed.
The purpose of the invention can be realized by the following technical scheme:
application of cholesterol substances in preparing additives or feeds for relieving influence of heat stress on oestrus rate and conception rate of sheep.
In a preferred embodiment of the present invention, the cholesterol is added in an amount of 0.2-1.0 g per ewe per day.
In a preferred embodiment of the present invention, the cholesterol-based substance is cholesterol or a cholesterol derivative having cholesterol activity.
A method for improving the oestrus rate and conception rate of sheep under heat stress comprises adding cholesterol substances into sheep feed or drinking water in an amount of 0.05-0.2%, and feeding sheep ewes in oestrus interval, oestrus period or 30 days after mating in high-temperature season.
Preferably, the sheep feed is selected from the group consisting of a complete mixed ration, a complete granulated feed, a concentrated feed, a concentrate feed, a premix feed and a feed raw material.
In a preferred embodiment of the present invention, the amount of said supplementary feeding cholesterol is 0.2-1.0 g/ewe per day.
In a preferred embodiment of the present invention, the cholesterol-based substance is cholesterol or a cholesterol derivative having cholesterol activity.
A sheep feed is prepared by adding 0.05-0.2% of cholesterol substances into a basic feed.
Preferably, the basic feed is selected from the group consisting of a complete mixed ration, a complete granulated feed, a concentrated feed, a concentrate feed, a premix feed and a feed raw material.
In a preferred embodiment of the present invention, the cholesterol-based substance is cholesterol or a cholesterol derivative having cholesterol activity.
Has the advantages that:
with the intensive development of sheep breeding and the aggravation of global warming, the adverse effect of heat stress on the reproductive performance of sheep becomes increasingly serious. Heat stress affects late development of sheep oocytes, embryonic development and implantation, pregnancy maintenance and fetal development, and is manifested by prolonged estrus cycle, decreased conception rate and decreased embryo survival rate. According to the invention, 0.2g-1.0 g/one day of cholesterol is added into the ewe feed or drinking water in the period from the estrus to 30 days after hybridization in a high-temperature season, so that the influence of heat stress on the estrus rate and the hybridization rate of sheep can be obviously relieved, the estrus rate and the conception rate of sheep are obviously improved, and the economic benefit of a farm is improved.
Drawings
FIG. 1 shows the total cholesterol level in the serum of different experimental sheep at 0h, 2h, 4h, 8h, 12h, 24h, 48h, 96h and 120h after feeding cholesterol
FIG. 2 Total Cholesterol levels in Hu sheep sera at different time points after Cholesterol supplementation
Detailed Description
The test methods mentioned in the following examples are conventional methods unless otherwise specified, and the total cholesterol assay kit is purchased from Nanjing Biotechnology engineering Ltd, the estradiol and progesterone assay kit is purchased from Beijing northern Biotechnology research institute Ltd, and the feed-grade cholesterol is purchased from Zhengzhou xanthus food chemical Co Ltd. Example 1 supplementary cholesterol feeding in summer to increase the cholesterol level in the serum of a Hu sheep (materials and methods):
(1) examples preparation of the required feed: the additive is added into the Hu sheep feed according to the addition amount of 0.1 percent and 0.5 percent of cholesterol, and the feed comprises 39.0 percent of corn, 4.3 percent of bran, 5.0 percent of rice bran, 9.0 percent of bean pulp, 3.0 percent of corn DDGS,3.0 percent of corn germ bean pulp, 32.0 percent of peanut stem powder, 3.0 percent of Zhengchang premix (fattening), 1.0 percent of baking soda and 0.7 percent of stone powder; the control feed contained no cholesterol.
(2)60 Hu sheep ewes of multiparous Hu are from Liuhe animal base of agricultural academy of sciences in Jiangsu province, and are healthy and infertile. Randomly divided into 3 groups. Three groups of sheep were fed with no cholesterol added feed (control group), 0.1% cholesterol added feed (0.1% test group) and 0.5% cholesterol added feed (0.5% test group), respectively.
(3) Three groups of experimental sheep respectively collected blood before feeding and after feeding for 2h, 4h, 8h, 12h, 24h, 48h, 96h and 120h, and serum was separated. The total cholesterol level in the serum of different experimental sheep is detected by ELISA method.
(II) results and analysis
(1) The supplementary feeding of cholesterol significantly improves the total cholesterol level in the hu sheep serum by the ELISA method, and the total cholesterol level in the hu sheep serum of the control group, the 0.1% experimental group and the 0.5% experimental group is detected. As a result, it was found that the total cholesterol levels in the sera of the Hu sheep in the 0.1% test group and the 0.5% test group were gradually increased from 2h to 48h after feeding and did not increase any more until 120h after 48h, compared to the control group, but the difference in the increase in cholesterol was insignificant between the 0.1% test group and the 0.5% test group (FIG. 1).
Example 2 method for increasing oestrus and conception rates of Hu sheep by supplementary feeding cholesterol in summer
Materials and methods:
(1) the test of the embodiment starts at 7 months and 14 days in 2021, ends at 9 months and 14 days in 2021, and the average temperature of Taicano city in Suzhou city of Jiangsu is higher than 25 ℃.
(2) Examples preparation of the required feed: the additive is added into the Hu sheep feed according to the addition of 0.1 percent of cholesterol, the feed comprises 39.0 percent of corn, 4.3 percent of bran, 5.0 percent of rice bran, 9.0 percent of soybean meal, 3.0 percent of corn DDGS,3.0 percent of corn germ soybean meal, 32.0 percent of peanut straw powder, 3.0 percent of Zhengchang premix (fattening), 1.0 percent of baking soda and 0.7 percent of stone powder, and the components are pressed into granular feed with the diameter of 6 mm; the control feed was a 6mm diameter pellet feed without 0.1% cholesterol.
(3)78 Hu sheep ewes of multiparous origin are from Donglin test sheep farm in Taicano city, Jiangsu province, and are healthy and infertile. Two groups of Hu sheep ewes were randomly divided into 2 groups and treated by conventional estrus synchronization, i.e., the ewes were placed in the vagina of the ewes with pessaries and removed on day 13 after placement and immediately injected intramuscularly with prostaglandin 1ml per sheep. On the day of estrus treatment, two groups of sheep were fed with feed without cholesterol and feed with cholesterol, respectively, and named as control group and cholesterol supplementary feeding group; feeding two groups of sheep with 500g pellet feed per sheep per day, and feeding cholesterol 0.5g per sheep per day
(4) The method comprises the steps of carrying out estrus test on Hu sheep subjected to estrus treatment in the same period, carrying out statistics on the estrus conditions of two groups in the first estrus period and the next natural estrus period, identifying the estrus conditions of the Hu sheep in a ram estrus test mode, and carrying out statistics on the estrus rates. And (3) carrying out artificial insemination and hybridization on the same semen on the ewes of the control group and the cholesterol supplementary feeding group, and detecting the progesterone content in serum 30 days after hybridization so as to judge the conception rate of the 2 groups of Hu sheep.
(5) The significant analysis of the estrus rate and conception rate difference of the control group and the supplementary feeding group is carried out by adopting the One-way ANOVA method in SPSS 19.0.
(II) results and analysis
(1) The content of cholesterol in serum of the cholesterol supplementary feeding group is obviously improved
The female sheep of the Hu sheep of the control group and the cholesterol supplementary feeding group are subjected to estrus synchronization treatment respectively, and the blood separated serum of the sheep of the cholesterol supplementary feeding group is collected respectively before supplementary feeding, on the 3 rd day and on the 30 th day of supplementary feeding. The change of the cholesterol content in the serum was detected by a total cholesterol assay kit. The results (fig. 2) found that the serum cholesterol levels in the hu sheep were significantly increased both on the third and 30 th days after the supplementary feeding of cholesterol (P <0.01) compared to before the supplementary feeding; .
(2) Supplementary feeding cholesterol to improve the estrus rate of Hu sheep
And adopting a ram cross estrus test to perform estrus identification on the Hu sheep ewes of the control group and the Cholesterol supplementary feeding group from 32 days to 34 days after the estrus treatment in the same period respectively, and finding that the estrus rate of the Hu sheep of the Cholesterol supplementary feeding group is 7.6 percent higher than that of the control group.
(3) Obviously improving the conception rate of Hu sheep by supplementing cholesterol
And (3) carrying out artificial insemination treatment on the female sheep of the Hu sheep in estrus in the result 2, collecting blood and separating serum after 30 days, detecting the content of progesterone in the serum of the Hu sheep in a control group and a cholesterol supplementary feeding group by an ELISA method, and confirming the conception rate. As a result, compared with the control group, the Hu sheep conception rate of the cholesterol supplementary feeding group is improved by 16.7%.
TABLE 1 Effect of cholesterol supplementation on the oestrus and conception rates of Hu sheep
| Control group | Experimental group | |
| Natural estrus rate | 82.7％ | 89.1％ |
| Conception rate | 46.9％a | 63.6％b |
Claims (10)
1. Application of cholesterol substances in preparing additives or feeds for relieving influence of heat stress on oestrus rate and conception rate of sheep.
2. The use according to claim 1, characterized in that the cholesterol substances are added in an amount of 0.2-1.0 g per ewe per day.
3. Use according to claim 1, characterized in that the cholesterol-like substance is cholesterol or a cholesterol derivative having cholesterol activity.
4. A method for improving the oestrus rate and the conception rate of sheep under heat stress is characterized in that cholesterol substances are added into sheep feed or drinking water in an adding amount of 0.05-0.2%, and the sheep ewes are fed in the oestrus period, the oestrus period or the period from hybridization to hybridization for 30 days in a high-temperature season.
5. The method as claimed in claim 1, wherein the sheep feed is selected from the group consisting of a complete mixed ration, a complete pellet, a concentrate feed, a premix feed and a feedstuff.
6. The method according to claim 1, wherein the amount of said supplementary feeding cholesterol is between 0.2 and 1.0g per ewe per day.
7. The method of claim 1, wherein the cholesterol is cholesterol or a cholesterol derivative having cholesterol activity.
8. The sheep feed is characterized in that 0.05-0.2% of cholesterol substances are added into a basic feed.
9. The sheep feed according to claim 8, wherein the basal feed is selected from the group consisting of a complete mixed ration, a complete pellet, a concentrate, a premix, and a feedstuff.
10. The sheep feed according to claim 1, characterized in that said cholesterol is cholesterol or a cholesterol derivative having cholesterol activity.
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