CN114591810A - Be used for bacterin culture apparatus - Google Patents

Be used for bacterin culture apparatus Download PDF

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Publication number
CN114591810A
CN114591810A CN202210361608.1A CN202210361608A CN114591810A CN 114591810 A CN114591810 A CN 114591810A CN 202210361608 A CN202210361608 A CN 202210361608A CN 114591810 A CN114591810 A CN 114591810A
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China
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fixedly connected
placing
tank body
plate
bottom end
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Withdrawn
Application number
CN202210361608.1A
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Chinese (zh)
Inventor
徐田放
殷成华
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Jiujiang Bo Lai Biological Products Co
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Jiujiang Bo Lai Biological Products Co
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Priority to CN202210361608.1A priority Critical patent/CN114591810A/en
Publication of CN114591810A publication Critical patent/CN114591810A/en
Withdrawn legal-status Critical Current

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    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals
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    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/06Tubular
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    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/50Means for positioning or orientating the apparatus
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • C12M29/08Air lift
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    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20Degassing; Venting; Bubble traps
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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Abstract

The invention relates to the technical field of vaccine culture, in particular to a vaccine culture device, which comprises: the tank comprises a tank body, wherein both sides of the surface of the tank body are fixedly connected with air cylinders, and the top ends of the air cylinders are fixedly connected with tank covers matched with the tank body; the placing plate is arranged in the tank body, and the inner wall of the placing plate is fixedly connected with a cross-shaped partition plate; first seal assembly and second seal assembly mutually support among the device, can realize the multiple sealed operation of jar body and cover, make and cultivate the complete sealed operation of ware in the inside realization of the jar body, thereby make the jar body realize a complete confined space, thereby to the temperature of jar internal portion, when cultivating the environment such as humidity and oxygen and regulating and control, it is more accurate, cultivate the body and can not receive external environment's influence, thereby ensure to cultivate the normal clear of work, and the process of this sealed operation is automatic going on when closing the cover, need not artificial independent operation, reduce the degree of difficulty of operation.

Description

Be used for bacterin culture apparatus
Technical Field
The invention relates to the technical field of vaccine culture, in particular to a vaccine culture device.
Background
The vaccine is a biological product which is prepared from various pathogenic microorganisms and is used for vaccination. Wherein the vaccine made with the bacteria or spirochetes is also called bacterin. The vaccines are divided into live vaccines and dead vaccines, the culture medium is a nutrient substrate which is prepared by combining different nutrient substances and is supplied for microorganisms, plants or animals (or tissues) to grow and reproduce, the production process of the vaccines needs the culture medium for culture, the sterilization process of the culture medium is extremely important, and the temperature and the humidity of the culture process need to be controlled.
The control of temperature and humidity all adopts current electrical components to control, but current bacterin breeding device sealing performance is relatively poor, can lead to the inside temperature of breeding device and humidity not well controlled, is difficult to keep the environment of cultivating of constant temperature and humidity, and breeding device is when placing simultaneously, because of placing the face out of level, can lead to bacterin to distribute inhomogeneously in cultivating the ware, influences the normal operation of cultivating of bacterin, adjusts inconvenient.
Disclosure of Invention
The invention aims to solve the problems that the sealing performance is poor, the temperature and humidity inside the culture device are not well controlled, the constant-temperature and constant-humidity culture environment is difficult to maintain, and the distribution of vaccines in a culture dish is not uniform due to the fact that the placing surface of the culture device is not horizontal when the culture device is placed, so that the normal culture operation of the vaccines is influenced, and the adjustment is inconvenient.
The invention achieves the above objects by the following technical scheme, and a device for culturing a vaccine comprises: the tank comprises a tank body, wherein both sides of the surface of the tank body are fixedly connected with air cylinders, and the top ends of the air cylinders are fixedly connected with tank covers matched with the tank body; the placing plate is arranged in the tank body, the inner wall of the placing plate is fixedly connected with a cross-shaped partition plate, and the top end of the placing plate is provided with a culture dish; the air inlet component is used for realizing uniform humidification, temperature control and oxygen operation in the tank body and is arranged on the inner wall of the tank body; the first sealing assembly is arranged between the tank body and the tank cover; the second sealing component is arranged on the inner wall of the tank body and further increases the sealing performance between the tank body and the tank cover; the horizontal component for realizing the horizontal placement of the culture dish is arranged at the bottom end of the placing plate; the lifting assembly is used for controlling the culture dish to enter and exit the tank body and is arranged at the bottom end of the second sealing assembly; the clamping assembly is convenient for the culture dish to take and limit the operation and is arranged in the cross-shaped partition plate.
Preferably, the subassembly that admits air is including rotating the intake pipe of connecting in the internal diapire of jar, the top of intake pipe runs through to jar body and is provided with cross communicating pipe, four ends of cross communicating pipe all are provided with the rotating tube, the surface of rotating tube is provided with the shower nozzle, the first motor of the bottom fixedly connected with of the jar body, the first conical gear of the equal fixedly connected with in surface of the output shaft of first motor and intake pipe, two a conical gear meshing is connected.
Preferably, first seal assembly includes the shift ring, first mounting groove and second mounting groove have been seted up from outer to interior in proper order on the top of the jar body, the shift ring sets up in first mounting groove, the first spring of fixedly connected with between shift ring and the first mounting groove, the top of shift ring is provided with first sealing washer, the inner diapire of first mounting groove is provided with first drive gasbag, the third mounting groove that two and be linked together with the second mounting groove is seted up to the inside of the jar body, the inner wall of third mounting groove is provided with the air-tight bag that is linked together with first drive gasbag, the bottom fixedly connected with and the first sealing ring of second mounting groove assorted of cover.
Preferably, the second seal assembly comprises an encryption cover fixedly connected with the bottom end of a cover body, the inner wall of the tank body is fixedly connected with an encryption ring matched with the encryption cover, a second seal ring is integrally formed on the inner edge of the encryption ring, a second seal ring is arranged on the top end of the second seal ring, and an extrusion ring matched with the second seal ring is arranged at the bottom end of the encryption cover.
Preferably, the lifting assembly is connected in the lead screw on the top of the encryption cover including rotating, the encryption cover is run through out to the bottom of lead screw, the surperficial threaded connection of lead screw has the thread bush, the surface of thread bush is articulated with the place board, the top fixedly connected with second motor of encryption cover, the equal fixedly connected with second conical gear in output shaft of second motor and the top of lead screw, two second conical gear meshing connects.
Preferably, the horizontal component comprises two telescopic rods fixedly connected to the bottom end of the thickening cover, the bottom end of each telescopic rod is fixedly connected with a horizontal plate, the inner wall of the tank body is fixedly connected with a connecting rod, the bottom end of each connecting rod is fixedly connected with a placing ring, the top end of each placing ring is provided with a placing seat which is uniformly distributed, the inside of each placing seat is provided with water solution and is adjacent to the inside of each placing seat, a first hose is arranged between the placing seats, the bottom end of each horizontal plate is provided with a round rod which is uniformly distributed and matched with the placing seats, the bottom end of each round rod is provided with a suspension plate, and the cross sectional area of each suspension plate is smaller than that of each placing seat.
Preferably, the bottom fixedly connected with drum of encryption lid, the interior diapire of drum is provided with second drive gasbag, the inner wall of drum is provided with the stripper plate, the bottom of stripper plate run through in proper order second drive gasbag and drum and with the top fixed connection of cross baffle, the surface of suspension plate all imbeds all around and installs the gas receiver, and is a plurality of communicate each other between the gas receiver, the inner wall one end fixedly connected with second spring of gas receiver, the other end fixedly connected with locating lever of second spring, the other end of locating lever runs through out the gas receiver and is provided with the rubber pad, the surface of second drive gasbag is provided with the second hose, the other end of second hose runs through drum and horizontal plate in proper order and leads to there are four third hoses, the other end and the gas receiver of third hose are linked together.
Preferably, the centre gripping subassembly includes fixed connection in the circular seat of placing the board bottom, the interior roof of circular seat is provided with third drive gasbag, two third springs of the interior roof fixedly connected with of circular seat, the bottom of third spring runs through out circular seat and fixedly connected with extrusion post, a plurality of bar grooves have been seted up to the inner wall of cross baffle, the inner wall in bar groove is provided with spacing gasbag, and is a plurality of spacing gasbag communicates each other, be provided with the fourth hose between spacing gasbag and the third drive gasbag.
Preferably, both sides of the top end of the placing plate are fixedly connected with supporting seats, the horizontal height of each supporting seat is lower than that of each circular seat, and the horizontal height of each supporting seat is higher than that of each extrusion column.
Preferably, the bottom fixedly connected with gag lever post of encryption lid, the surperficial sliding connection of gag lever post has the sliding block, the sliding block is articulated with the surface of placing the board.
The beneficial effects of the invention are:
1. through the arrangement of the first sealing assembly and the second sealing assembly, when the tank cover moves downwards, the first sealing ring can enter the second mounting groove firstly, in the continuous moving process, the bottom end of the tank cover can be in contact with the first sealing ring and drive the moving ring to move downwards in the first mounting groove, the first spring is stressed and bent, the first driving air bag is extruded at the same time, the first driving air bag is pressed under the influence of air pressure, air can directly enter the sealing air bag, the sealing air bag expands to penetrate through the third mounting groove and be in surface contact with the first sealing ring, sealing operation is realized, the sealing cover can simultaneously move downwards along with the tank cover and be attached with the encryption ring, the extrusion ring can be in complete contact with the second sealing ring, the first sealing assembly and the second sealing assembly are matched with each other, multiple sealing operation of the tank body and the tank cover can be realized, and complete sealing operation of the culture dish in the tank body can be realized, therefore, a complete sealed space is realized in the tank body, so that when the cultivation environments such as temperature, humidity and oxygen in the tank body are regulated and controlled, the cultivation bodies are more accurate and cannot be influenced by the external environment, the normal operation of the cultivation work is ensured, the process of the sealing operation is automatically performed when the tank cover is closed, manual independent operation is not needed, and the operation difficulty is reduced;
2. through the arrangement of the horizontal assembly, when the tank cover moves in place, the placing seats can be contacted with the top of the placing ring under the action of the telescopic rod, under the action of the first hose, water in the four placing seats can be automatically and horizontally adjusted according to the inclination angle, the suspension plate is influenced by the water plane and always floats on the liquid level of the placing seats, so that the horizontal adjustment operation of the horizontal plate is realized, when the liquid level is kept still for a period of time without fluctuation, the lifting assembly can drive the placing plate to move downwards, the extrusion plate can synchronously move downwards along with the placing plate, the cylinder and the encryption cover synchronously keep still, the extrusion plate can extrude the second driving air bag, gas in the second driving air bag can enter the second hose and is conveyed into the plurality of air storage cylinders through the third hose, the gas is pushed to the positioning rod to move, and the positioning of the horizontal plate is realized by utilizing the contact of the rubber pads and the inner wall of the placing seats, the gas has the compression performance, the stability of the horizontal component is improved by further extruding the gas, the horizontal component is leveled after the liquid level is static, the horizontal component can automatically adjust the horizontal plane when the tank cover is closed, and the reinforcing and limiting operation is carried out after the standing time is up, so that the placing plate and the culture dish are leveled, the reasonable and effective culture of the culture body in the culture dish is realized, the normal culture work of the culture body is prevented from being influenced when the tank body is placed in an inclined state, and the mode is simpler to adjust;
3. by arranging the lifting assembly, starting the second motor, under the action of the two second bevel gears, the screw rod rotates in situ to drive the threaded sleeve to move on the surface of the screw rod to generate vertical height, so that the position of the placing plate is adjusted, the lifting assembly can adjust the height of the placing plate to a certain degree, and the lifting assembly is combined with the cylinder for use, so that the placing plate and the culture dish can be moved to the outside of the tank body, therefore, the placing and taking of the culture body are more convenient during the culture operation, and the placing work of the placing plate is performed after the horizontal assembly is horizontally realized, so that the adjusting effect of the horizontal assembly is better;
4. through setting up the centre gripping subassembly, place the board when the downstream, the extrusion post can be at first with the horizontal plate contact, at the in-process of placing the board continuous movement, the third spring can be atress crooked, the extrusion post gets into to circular seat in, extrude third drive gasbag, can carry to spacing gasbag through the fourth hose among the third drive gasbag, spacing gasbag takes place to expand and runs through out the bar groove and cultivates the contact of ware, realize cultivating the spacing of ware placing between board and the cross baffle, the centre gripping subassembly can be according to the state automatic switch-over state of placing between board and the horizontal component, when placing board and horizontal component and connecting, increase the stability of placing the board, thereby reduce external vibrations factor influence and cultivate the body, when placing board and horizontal component and not connecting, can remove the locking automatically, be convenient for the operation of taking of cultivating the ware.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the present invention;
FIG. 3 is a schematic structural view of a canister and air intake assembly of the present invention;
FIG. 4 is a schematic view of the construction of a first seal assembly and a second seal assembly of the present invention;
FIG. 5 is a schematic structural view of a canister and a first seal assembly of the present invention;
FIG. 6 is a schematic view of the second sealing assembly, the lifting assembly and the placing plate according to the present invention;
FIG. 7 is a schematic view of the arrangement of the holding plate and the clamping assembly of the present invention;
FIG. 8 is a schematic structural view of a horizontal assembly of the present invention;
fig. 9 is a schematic view of the internal structure of the seat of the present invention.
In the figure: 1. a tank body; 101. a first mounting groove; 102. a second mounting groove; 103. a third mounting groove; 2. a cylinder; 3. a can lid; 4. placing the plate; 401. a supporting seat; 5. a cross-shaped partition plate; 501. a strip-shaped groove; 6. a culture dish; 7. an air intake assembly; 701. an air inlet pipe; 702. a cross connection pipe; 703. rotating the tube; 704. a spray head; 705. a first motor; 706. a first bevel gear; 8. a first seal assembly; 801. a moving ring; 802. a first spring; 803. a first seal ring; 804. a first drive airbag; 805. sealing the air bag; 806. a first seal ring; 9. a second seal assembly; 901. an encryption cover; 902. encrypting the ring; 903. a second seal ring; 904. a second seal ring; 905. an extrusion ring; 10. a horizontal component; 1001. a telescopic rod; 1002. a horizontal plate; 1003. a connecting rod; 1004. placing a ring; 1005. a placing seat; 1006. a first hose; 1007. a round bar; 1008. a suspension plate; 1009. a cylinder; 1010. a second drive airbag; 1011. a pressing plate; 1012. an air cylinder; 1013. a second spring; 1014. positioning a rod; 1015. a rubber pad; 1016. a second hose; 1017. a third hose; 11. a lifting assembly; 1101. a screw rod; 1102. a threaded sleeve; 1103. a second motor; 1104. a second bevel gear; 1105. a limiting rod; 12. a clamping assembly; 1201. a circular seat; 1202. a third driving airbag; 1203. a third spring; 1204. extruding the column; 1205. a limiting air bag; 1206. and a fourth hose.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the specific implementation: as shown in fig. 1-9, a device for vaccine culture, comprising: the tank comprises a tank body 1, wherein both sides of the surface of the tank body 1 are fixedly connected with cylinders 2, and the top ends of the cylinders 2 are fixedly connected with tank covers 3 matched with the tank body 1; the tank comprises a placing plate 4, the placing plate 4 is arranged in a tank body 1, the inner wall of the placing plate 4 is fixedly connected with a cross-shaped partition plate 5, and the top end of the placing plate 4 is provided with a culture dish 6; the air inlet component 7 is used for realizing uniform humidification, temperature control and oxygen operation in the tank body 1, and the air inlet component 7 is arranged on the inner wall of the tank body 1; a first sealing assembly 8 arranged between the can body 1 and the can lid 3; the second sealing component 9, the second sealing component 9 which further increases the sealing performance between the tank body 1 and the tank cover 3 is arranged on the inner wall of the tank body 1; a horizontal member 10 provided at the bottom end of the placing plate 4 with the horizontal member 10 for horizontally placing the cultivation dish 6; the lifting component 11 is used for controlling the lifting component 11 of the culture dish 6 to enter and exit the tank body 1 and is arranged at the bottom end of the second sealing component 9; a clamping component 12, the clamping component 12 which is convenient for taking and limiting the culture dish 6 is arranged in the cross-shaped clapboard 5, when the vaccine is cultured, a culture body is placed in the culture dish 6, the cylinder 2 is started, the cylinder 2 is utilized to drive the tank cover 3 to move downwards and close the tank body 1, in the process, the first sealing component 8 and the second sealing component 9 can automatically operate to realize the completely closed operation of the tank body 1 and the tank cover 3, meanwhile, in the process, the horizontal component 10 can realize the combination of the tank body 1, after the device is placed for a period of time, the horizontal component 10 can automatically adjust the time, after the adjusted level is reached, the lifting component 11 is started to realize the placement of the placing plate 4 on the horizontal component 10, in the process of placing the plate 4 descending, the limiting and locking operation of the horizontal component 10 is automatically completed, when the placing plate 4 continuously descends and is completely placed on the horizontal component 10, the clamping component 12 can automatically operate in the process, so that the culture dish 6 is stably placed on the placing plate 4, when the internal environments of the tank body 1 such as temperature, humidity and oxygen need to be adjusted, the air inlet component 7 can be used for adding operation, the first sealing component 8 and the second sealing component 9 in the device are matched with each other, multiple sealing operation of the tank body 1 and the tank cover 3 can be realized, the culture dish 6 can realize complete sealing operation in the tank body 1, so that the tank body 1 can realize a complete sealing space, when the internal environments of the tank body 1 such as temperature, humidity and oxygen are adjusted, the culture body is more accurate, the culture body cannot be influenced by the external environment, the normal operation of the culture work is ensured, the sealing operation process is automatically performed when the tank cover 3 is closed, and no manual independent operation is needed, the difficulty of operation is reduced, the horizontal component 10 is horizontal after the liquid level is static, the horizontal component 10 can automatically adjust the horizontal plane when the tank cover 3 is closed, after the standing time is up, the reinforcing and limiting operation is carried out, so that the placing plate 4 and the culture dish 6 are horizontal, the reasonable and effective culture of the culture body in the culture dish 6 is realized, the normal culture work of the culture body is prevented from being influenced when the tank body 1 is placed in an inclined state, the mode is simple to adjust, the lifting component 11 can adjust the height of the placing plate 4 to a certain degree, the lifting component and the cylinder 2 are combined for use, the placing plate 4 and the culture dish 6 can be moved to the outside of the tank body 1, the culture body can be placed and taken conveniently during the culture work, the placing work of the placing plate 4 is carried out after the horizontal component 10 is horizontal, and the adjusting effect of the horizontal component 10 is better, centre gripping subassembly 12 can be according to placing the state automatic switch-over state between board 4 and the horizontal component 10, when placing board 4 and horizontal component 10 and connect, increases the stability of placing board 4 to reduce external vibrations factor and influence and cultivate the body, place board 4 and horizontal component 10 and when not throw out of gear, can remove the locking automatically, be convenient for cultivate the operation of taking of ware 6.
As shown in fig. 1, fig. 2, fig. 3, fig. 4, fig. 5 and fig. 6, the air inlet assembly 7 includes an air inlet pipe 701 rotatably connected to the inner bottom wall of the tank 1, the top end of the air inlet pipe 701 penetrates through the tank 1 and is provided with a cross connection pipe 702, four ends of the cross connection pipe 702 are provided with rotation pipes 703, a spray nozzle 704 is arranged on the surface of the rotation pipe 703, the bottom end of the tank 1 is fixedly connected with a first motor 705, an output shaft of the first motor 705 and the surface of the air inlet pipe 701 are both fixedly connected with a first bevel gear 706, and the two first bevel gears 706 are meshed and connected with each other, when the cultivation environment inside the tank 1 needs to be adjusted, factors such as humidity, temperature and oxygen can enter the cross connection pipe 702 from the air inlet pipe 701 according to requirements, then enter the rotation pipe 703 and be sprayed into the tank 1 by the spray nozzle 704, and the first motor 705 is started at the same time, under the action of the two first bevel gears 706, the air inlet pipe 701, the cross communicating pipe 702 and the rotating pipe 703 automatically rotate, so that the environment inside the tank body 1 is more uniform, and the uniform regulation and control of the environment inside the tank body 1 are realized. The first sealing assembly 8 comprises a moving ring 801, a first mounting groove 101 and a second mounting groove 102 are sequentially formed in the top end of the tank body 1 from outside to inside, the moving ring 801 is arranged in the first mounting groove 101, a first spring 802 is fixedly connected between the moving ring 801 and the first mounting groove 101, a first sealing ring 803 is arranged at the top end of the moving ring 801, a first driving air bag 804 is arranged on the inner bottom wall of the first mounting groove 101, two third mounting grooves 103 communicated with the second mounting groove 102 are formed in the tank body 1, a sealing air bag 805 communicated with the first driving air bag 804 is arranged on the inner wall of the third mounting groove 103, a first sealing ring 806 matched with the second mounting groove 102 is fixedly connected to the bottom end of the tank cover 3, when the tank cover 3 moves downwards, the first sealing ring 806 enters the second mounting groove 102 first, and in the continuous moving process, the bottom end of the tank cover 3 contacts with the first sealing ring 803, and drives the movable ring 801 to move downwards in the first mounting groove 101, the first spring 802 is stressed and bent to simultaneously press the first driving air bag 804, the first driving air bag 804 is pressed under the influence of air pressure, air can directly enter the sealing air bag 805, the sealing air bag 805 expands to penetrate through the third mounting groove 103 and contact with the surface of the first sealing ring 806 to realize sealing operation, the second sealing assembly 9 comprises an encryption cover 901 fixedly connected with the bottom end of the tank cover 3, the inner wall of the tank body 1 is fixedly connected with an encryption ring 902 matched with the encryption cover 901, the inner edge of the encryption ring 902 is integrally formed with a second sealing ring 903, the top end of the second sealing ring 903 is provided with a second sealing ring 904, the bottom end of the encryption cover 901 is provided with an extrusion ring 905 matched with the second sealing ring 903, simultaneously the encryption cover 901 can move downwards along with the tank cover 3 and be attached with the encryption ring, the extrusion ring 905 can be completely contacted with the second sealing ring 904, first seal assembly 8 and second seal assembly 9 mutually support, can realize the multiple sealed operation of jar body 1 and cover 3, make and cultivate ware 6 and realize complete sealed operation in the inside of jar body 1, thereby make jar body 1 realize a complete sealed space, thereby to the temperature of jar body 1 inside, when cultivating the environment such as humidity and oxygen and regulating and control, it is more accurate, cultivate the body and can not receive external environment's influence, thereby ensure to cultivate the normal clear of work, and the process of this sealed operation is automatic going on when closing cover 3, need not artificial independent operation, reduce the degree of difficulty of operation.
As shown in fig. 1, 2, 6, 7, 8 and 9, the lifting assembly 11 includes a screw 1101 rotatably connected to the top end of the encryption cover 901, the bottom end of the screw 1101 penetrates through the encryption cover 901, a threaded sleeve 1102 is threadedly connected to the surface of the screw 1101, the surface of the threaded sleeve 1102 is hinged to the placing plate 4, a second motor 1103 is fixedly connected to the top end of the encryption cover 901, a second bevel gear 1104 is fixedly connected to an output shaft of the second motor 1103 and the top end of the screw 1101, the two bevel gears 1104 are engaged with each other, the second motor 1103 is started, the screw 1101 rotates in situ under the action of the two bevel gears 1104 to drive the threaded sleeve 1102 to move vertically on the surface of the screw 1101, so as to adjust the position of the placing plate 4, the lifting assembly 11 can adjust the height of the placing plate 4 to a certain extent, and is used in combination with the air cylinder 2, i.e. the placing plate 4 and the culture dish 6 can be moved to the outside of the tank 1, therefore, when the cultivation operation is carried out, the cultivation bodies can be placed and taken more conveniently, the horizontal component 10 is horizontally placed, and then the placing work of the plate 4 is carried out, so that the adjusting effect of the horizontal component 10 is better. The horizontal component 10 comprises two telescopic rods 1001 fixedly connected to the bottom end of the encryption cover 901, the bottom end of the telescopic rod 1001 is fixedly connected with a horizontal plate 1002, the inner wall of the tank body 1 is fixedly connected with a connecting rod 1003, the bottom end of the connecting rod 1003 is fixedly connected with a placing ring 1004, the top end of the placing ring 1004 is provided with uniformly distributed placing seats 1005, aqueous solution is arranged inside the placing seats 1005, a first hose 1006 is arranged between every two adjacent placing seats 1005, the bottom end of the horizontal plate 1002 is provided with uniformly distributed round rods 1007 matched with the placing seats 1005, the bottom end of the round rods 1007 is provided with a suspension plate 1008, the cross-sectional area of the suspension plate 1008 is smaller than that of the placing seats 1005, the bottom end of the encryption cover 901 is fixedly connected with a cylinder 1009, the inner bottom wall of the cylinder 1009 is provided with a second driving air bag 1010, the inner wall of the cylinder 1009 is provided with a squeezing plate 1011, the bottom end of the squeezing plate 1011 sequentially penetrates through the second driving air bag 1010 and the cylinder 1009 and is fixedly connected with the top end of the cross-shaped clapboard 5, the air cylinders 1012 are embedded into and mounted on the periphery of the surface of the floating plate 1008, the air cylinders 1012 are communicated with one another, one end of the inner wall of each air cylinder 1012 is fixedly connected with a second spring 1013, the other end of each second spring 1013 is fixedly connected with a positioning rod 1014, the other end of each positioning rod 1014 penetrates through each air cylinder 1012 and is provided with a rubber pad 1015, the surface of the second driving air bag 1010 is provided with a second hose 1016, the other end of each second hose 1016 sequentially penetrates through the corresponding cylinder 1009 and the corresponding horizontal plate 1002 and is communicated with four third hoses 1017, the other end of each third hose 1017 is communicated with the corresponding air cylinder 1012, when the tank cover 3 is moved in place, the placing seat 1005 can be contacted with the top of the placing ring 1004 under the action of the telescopic rod 1001, under the action of the first hose 1006, water in the four placing seats 1005 can be automatically horizontally adjusted according to the inclination angles, the floating plate 1008 is influenced by the horizontal plane and always floats on the liquid surface of the placing seat 1005, realize the horizontal adjustment operation of the horizontal plate 1002, when the liquid level is not fluctuated any more after standing for a period of time, the lifting assembly 11 can drive the placing plate 4 to move downwards, the extruding plate 1011 can move downwards along with the placing plate 4 synchronously, the cylinder 1009 and the encryption cover 901 keep still synchronously, the extruding plate 1011 can extrude the second driving airbag 1010, the gas in the second driving airbag 1010 can enter the second hose 1016 and is conveyed to a plurality of gas storage cylinders 1012 through the third hose 1017, the gas pushes the positioning rod 1014 to move, the rubber gasket 1015 is contacted with the inner wall of the placing seat 1005 to realize the positioning of the horizontal plate 1002, the gas has the compression performance, the further extruding gas can increase the stability of the horizontal assembly 10, the horizontal assembly 10 can achieve the horizontal method after the liquid level is static, the horizontal assembly 10 can automatically adjust the horizontal plane when closing the tank cover 3, reinforce spacing operation after the time of standing to play and place board 4 and culture dish 6 and be in the level, realize cultivating the reasonable effective cultivation of the body in culture dish 6, can influence the normal work of cultivating the body when avoiding taking place the slope when the jar body 1 is placed, and this mode is adjusted comparatively simply. The clamping assembly 12 comprises a circular base 1201 fixedly connected to the bottom end of the placing plate 4, a third driving air bag 1202 is arranged on the inner top wall of the circular base 1201, two third springs 1203 are fixedly connected to the inner top wall of the circular base 1201, the bottom end of each third spring 1203 penetrates through the circular base 1201 and is fixedly connected with an extrusion column 1204, a plurality of strip-shaped grooves 501 are formed in the inner wall of the cross-shaped partition plate 5, a limiting air bag 1205 is arranged on the inner wall of each strip-shaped groove 501, the limiting air bags 1205 are communicated with each other, a fourth hose 1206 is arranged between each limiting air bag 1205 and the corresponding third driving air bag 1202, when the placing plate 4 moves downwards, the extrusion columns 1204 can firstly contact with the horizontal plate 1002, in the continuous moving process of the placing plate 4, the third springs 1203 can be stressed and bent, the extrusion columns 1204 enter the circular base 1201 and extrude the third driving air bags 1202, and air in the third driving air bags 1202 can be conveyed to the limiting air bags 1205 through the fourth hoses 1206, spacing gasbag 1205 takes place the inflation to run through out bar groove 501 and cultivates the contact of ware 6, realize cultivating ware 6 spacing placing between board 4 and cross baffle 5, centre gripping subassembly 12 can be according to the state automatic switch-over state of placing between board 4 and the horizontal component 10, when placing board 4 and the horizontal component 10 and connecting, increase the stability of placing board 4, thereby reduce external vibrations factor and influence the cultivation body, when placing board 4 and the horizontal component 10 and do not connect, can remove the locking automatically, be convenient for cultivate the operation of taking of ware 6. The supporting seats 401 are fixedly connected to two sides of the top end of the placing plate 4, the horizontal height of each supporting seat 401 is lower than that of the corresponding round seat 1201, and the horizontal height of each supporting seat 401 is higher than that of the corresponding extrusion column 1204, so that normal operation of the clamping assembly 12 is guaranteed. The bottom fixedly connected with gag lever post 1105 of encryption lid 901, the surperficial sliding connection of gag lever post 1105 has the sliding block, the sliding block with place the surface of board 4 articulated, sliding block and thread bush 1102 with place board 4 and be articulated, when placing board 4 and horizontal plate 1002 contact, two supporting seats 401 can stop on horizontal plate 1002, because of articulated mode is connected, place board 4 and can carry out automatically regulated to the laminating operation of board 4 and horizontal plate 1002 is placed in the realization.
When the vaccine culturing device is used, a culturing body is placed in a culturing dish 6, an air cylinder 2 is started, the air cylinder 2 is used for driving a tank cover 3 to move downwards and close a tank body 1, in the process, a first sealing assembly 8 and a second sealing assembly 9 automatically operate, when the tank cover 3 moves downwards, a first sealing ring 806 enters a second mounting groove 102 firstly, in the continuous moving process, the bottom end of the tank cover 3 is in contact with the first sealing ring 803 and drives a moving ring 801 to move downwards in a first mounting groove 101, a first spring 802 is stressed and bent and presses a first driving air bag 804, the first driving air bag 804 is pressed under the influence of air pressure, air directly enters the sealing air bag 805, the sealing air bag 805 expands to penetrate through a third mounting groove 103 and is in surface contact with the first sealing ring 806, sealing operation is realized, and a sealing cover 901 can move downwards and attach with an encryption ring 902 simultaneously along with the tank cover 3, the extrusion ring 905 can be in complete contact with the second sealing ring 904, meanwhile, in the process, the horizontal assembly 10 can realize the combination of the interior of the tank body 1, when the tank cover 3 moves in place, under the action of the telescopic rod 1001, the placing seat 1005 can be in contact with the top of the placing ring 1004, under the action of the first hose 1006, water in the four placing seats 1005 can be automatically and horizontally adjusted according to the inclination angle, the suspension plate 1008 is influenced by the water level and always floats on the liquid level of the placing seat 1005, so that the horizontal adjustment operation of the horizontal plate 1002 is realized, when the liquid level is kept still for a period of time, the lifting assembly 11 can drive the placing plate 4 to move downwards, the extrusion plate 1011 can synchronously move downwards along with the placing plate 4, the cylinder 1009 and the encryption cover 901 are synchronously kept still, the extrusion plate 1011 can extrude the second driving airbag 1010, gas in the second driving airbag 1010 can enter the second hose 1016, then the gas is conveyed to a plurality of gas storage cylinders 1012 through a third hose 1017, the gas is pushed to a positioning rod 1014 to move, the rubber gasket 1015 is contacted with the inner wall of a placing seat 1005, the positioning of the horizontal plate 1002 is realized, the gas has the compression performance, the gas is further extruded to increase the stability of the horizontal assembly 10, the lifting assembly 11 is started, under the action of a second motor 1103 and two second bevel gears 1104, the screw 1101 rotates in situ to drive the threaded sleeve 1102 to move vertically on the surface of the screw 1101, so that the position of the placing plate 4 is adjusted, the placing plate 4 on the horizontal assembly 10 is realized, in the descending process of the placing plate 4, the limiting and locking operation of the horizontal assembly 10 is automatically completed, when the placing plate 4 is continuously descended and completely placed on the horizontal assembly 10, the clamping assembly 12 automatically operates in the process, and when the placing plate 4 moves downwards, the extrusion column 1204 will contact with the horizontal plate 1002 first, in the process of placing the plate 4 and moving continuously, the third spring 1203 will be forced and bent, the extrusion column 1204 enters into the circular seat 1201, extrude the third driving gasbag 1202, the gas in the third driving gasbag 1202 can be conveyed to the spacing gasbag 1205 through the fourth hose 1206, the spacing gasbag 1205 expands and runs through the strip-shaped groove 501 and contacts with the culture dish 6, realize the spacing of the culture dish 6 between the placing plate 4 and the cross-shaped partition plate 5, when needing to adjust the internal environment of the tank body 1 such as temperature, humidity and oxygen, the air intake component 7 can be used for adding operation, the gas can enter into the cross-shaped communicating pipe 702 from the gas inlet pipe 701 according to the requirement, then enter into the rotating pipe, and be sprayed into the tank body 1 by the spray head 704, and the first motor 705 is started at the same time, under the action of the two first bevel gears 706, the gas inlet pipe 701,703,703, The cross connection pipe 702 and the rotating pipe 703 rotate automatically, so that the environment inside the tank body 1 is more uniform, and the uniform regulation and control of the environment inside the tank body 1 are realized.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (10)

1. A device for vaccine culture, comprising:
the tank comprises a tank body (1), wherein both sides of the surface of the tank body (1) are fixedly connected with cylinders (2), and the top ends of the cylinders (2) are fixedly connected with tank covers (3) matched with the tank body (1);
the tank comprises a placing plate (4), wherein the placing plate (4) is arranged in a tank body (1), the inner wall of the placing plate (4) is fixedly connected with a cross-shaped partition plate (5), and the top end of the placing plate (4) is provided with a culture dish (6);
the air inlet assembly (7) is used for realizing uniform humidification, temperature control and oxygen operation in the tank body (1), and the air inlet assembly (7) is arranged on the inner wall of the tank body (1);
the first sealing assembly (8) is arranged between the tank body (1) and the tank cover (3);
the second sealing assembly (9) is arranged on the inner wall of the tank body (1), and the sealing performance between the tank body (1) and the tank cover (3) is further improved;
the horizontal component (10) is arranged at the bottom end of the placing plate (4) by the horizontal component (10) for horizontally placing the culture dish (6);
the lifting assembly (11) is used for controlling the culture dish (6) to enter and exit the tank body (1), and the lifting assembly (11) is arranged at the bottom end of the second sealing assembly (9);
the clamping assembly (12) is convenient for the culture dish (6) to take and limit the operation, and the clamping assembly (12) is arranged in the cross-shaped partition plate (5).
2. A device for vaccine cultivation according to claim 1, characterized in that: air inlet assembly (7) are including rotating intake pipe (701) of connecting in jar body (1) interior diapire, the top of intake pipe (701) is run through to jar body (1) and is provided with cross communicating pipe (702), the four ends of cross communicating pipe (702) all are provided with rotating tube (703), the surface of rotating tube (703) is provided with shower nozzle (704), the bottom fixedly connected with first motor (705) of jar body (1), the output shaft of first motor (705) and the equal fixedly connected with first conical gear (706) in surface of intake pipe (701), two first conical gear (706) meshing is connected.
3. A device for vaccine cultivation according to claim 1, characterized in that: the first sealing component (8) comprises a moving ring (801), the top end of the tank body (1) is sequentially provided with a first mounting groove (101) and a second mounting groove (102) from outside to inside, the moving ring (801) is arranged in the first mounting groove (101), a first spring (802) is fixedly connected between the moving ring (801) and the first mounting groove (101), a first sealing ring (803) is arranged at the top end of the movable ring (801), a first driving air bag (804) is arranged on the inner bottom wall of the first mounting groove (101), two third mounting grooves (103) communicated with the second mounting groove (102) are formed in the tank body (1), the inner wall of the third mounting groove (103) is provided with a sealing air bag (805) communicated with the first driving air bag (804), the bottom end of the tank cover (3) is fixedly connected with a first sealing ring (806) matched with the second mounting groove (102).
4. A device for vaccine cultivation according to claim 1, characterized in that: the second sealing assembly (9) comprises an encryption cover (901) fixedly connected with the bottom end of the tank cover (3), the inner wall of the tank body (1) is fixedly connected with an encryption ring (902) matched with the encryption cover (901), a second sealing ring (903) is integrally formed on the inner edge of the encryption ring (902), a second sealing ring (904) is arranged at the top end of the second sealing ring (903), and a squeezing ring (905) matched with the second sealing ring (903) is arranged at the bottom end of the encryption cover (901).
5. A device for vaccine cultivation according to claim 4, characterized in that: the lifting assembly (11) comprises a screw rod (1101) rotatably connected to the top end of the encryption cover (901), the bottom end of the screw rod (1101) penetrates through the encryption cover (901), a threaded sleeve (1102) is connected to the surface of the screw rod (1101) in a threaded mode, the surface of the threaded sleeve (1102) is hinged to the placement plate (4), a second motor (1103) is fixedly connected to the top end of the encryption cover (901), a second bevel gear (1104) is fixedly connected to the output shaft of the second motor (1103) and the top end of the screw rod (1101), and the two second bevel gears (1104) are meshed and connected.
6. A device for vaccine cultivation according to claim 1, characterized in that: the horizontal component (10) comprises two telescopic rods (1001) fixedly connected to the bottom end of the thickening cover (901), a horizontal plate (1002) is fixedly connected to the bottom end of each telescopic rod (1001), a connecting rod (1003) is fixedly connected to the inner wall of the tank body (1), a placing ring (1004) is fixedly connected to the bottom end of each connecting rod (1003), uniformly distributed placing seats (1005) are arranged at the top end of each placing ring (1004), a water solution is arranged inside each placing seat (1005), a first hose (1006) is arranged between every two adjacent placing seats (1005), round rods (1007) which are uniformly distributed and matched with the placing seats (1005) are arranged at the bottom end of each horizontal plate (1002), a suspension plate (1008) is arranged at the bottom end of each round rod (1007), and the cross sectional area of each suspension plate (1008) is smaller than the cross sectional area of each placing seat (1005).
7. A device for vaccine cultivation according to claim 6, characterized in that: the bottom end of the encryption cover (901) is fixedly connected with a cylinder (1009), a second driving air bag (1010) is arranged on the inner bottom wall of the cylinder (1009), an extrusion plate (1011) is arranged on the inner wall of the cylinder (1009), the bottom end of the extrusion plate (1011) sequentially penetrates through the second driving air bag (1010) and the cylinder (1009) and is fixedly connected with the top end of a cross-shaped partition plate (5), air storage cylinders (1012) are embedded and installed on the periphery of the surface of the suspension plate (1008), the air storage cylinders (1012) are communicated with one another, one end of the inner wall of the air storage cylinder (1012) is fixedly connected with a second spring (1013), the other end of the second spring (1013) is fixedly connected with a positioning rod (1014), the other end of the positioning rod (1014) penetrates through the air storage cylinder (1012) and is provided with a rubber pad (1015), and a second hose (1016) is arranged on the surface of the second driving air bag (1010), the other end of the second hose (1016) penetrates through the cylinder (1009) and the horizontal plate (1002) in sequence and is communicated with four third hoses (1017), and the other end of each third hose (1017) is communicated with the air cylinder (1012).
8. A device for vaccine cultivation according to claim 1, characterized in that: the clamping assembly (12) comprises a circular base (1201) fixedly connected to the bottom end of the placing plate (4), the inner top wall of the circular base (1201) is provided with a third driving air bag (1202), two third springs (1203) fixedly connected to the inner top wall of the circular base (1201), the bottom end of each third spring (1203) penetrates through the circular base (1201) and the corresponding fixedly connected extrusion column (1204), the inner wall of the cross-shaped partition plate (5) is provided with a plurality of strip-shaped grooves (501), the inner walls of the strip-shaped grooves (501) are provided with limiting air bags (1205) and a plurality of the limiting air bags (1205) are communicated with each other, and fourth hoses (1206) are arranged between the limiting air bags (1205) and the third driving air bags (1202).
9. A device for vaccine cultivation according to claim 8, characterized in that: the two sides of the top end of the placing plate (4) are fixedly connected with supporting seats (401), the horizontal height of each supporting seat (401) is lower than that of the corresponding round seat (1201), and the horizontal height of each supporting seat (401) is higher than that of the corresponding extrusion column (1204).
10. A device for vaccine cultivation according to claim 4, characterized in that: the bottom end of the encryption cover (901) is fixedly connected with a limiting rod (1105), the surface of the limiting rod (1105) is connected with a sliding block in a sliding mode, and the sliding block is hinged with the surface of the placing plate (4).
CN202210361608.1A 2022-04-07 2022-04-07 Be used for bacterin culture apparatus Withdrawn CN114591810A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210361608.1A CN114591810A (en) 2022-04-07 2022-04-07 Be used for bacterin culture apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210361608.1A CN114591810A (en) 2022-04-07 2022-04-07 Be used for bacterin culture apparatus

Publications (1)

Publication Number Publication Date
CN114591810A true CN114591810A (en) 2022-06-07

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210361608.1A Withdrawn CN114591810A (en) 2022-04-07 2022-04-07 Be used for bacterin culture apparatus

Country Status (1)

Country Link
CN (1) CN114591810A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115746998A (en) * 2022-11-08 2023-03-07 杭州华端生物科技有限公司 Anaerobic culture tank

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115746998A (en) * 2022-11-08 2023-03-07 杭州华端生物科技有限公司 Anaerobic culture tank

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