CN114588191A - Preparation and application of Tibetan lithospermum extract - Google Patents
Preparation and application of Tibetan lithospermum extract Download PDFInfo
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Abstract
The invention relates to an effective extract of Tibetan lithospermum with the functions of stopping bleeding and promoting wound healing and a preparation method thereof.
Description
Technical Field
The invention discloses a preparation method of a Tibetan lithospermum extract for stopping bleeding and promoting wound healing and application of the Tibetan lithospermum extract in stopping bleeding and promoting wound healing, belonging to the field of national medicines.
Background
Shi Zi is originally recorded in Shen nong Ben Cao Jing, a commonly used Chinese medicine with sweet, salty and cold nature. It enters heart and liver meridians and has the functions of cooling blood, promoting blood circulation, removing toxicity and promoting eruption. Modern medical research shows that the traditional Chinese medicine composition has the effects of resisting tumors, inhibiting bacteria, resisting fertility, resisting viruses, protecting the liver, reducing blood sugar, resisting inflammation, calming, detoxifying, promoting eruption, regulating immunity, promoting wound healing and the like. The arnebia root has many varieties, including Sinkiang arnebia root (A)Arnebiaeuchroma(Royle) Johnst., lithospermum rimosus (Armebia guttataBunge, Lang Zi (root of Chinese wolf's sinkiang)LycopsisorientalisLinn.), Zi Cao (Lithospermum erythrorhizon Sieb et Zucc. (Lithospermum erythrorhizon Sieb.) (Lithospermum erythrorhizon) Radix Arnebiae seu LithospermiOnosmapaniculatum BurEt Franch, Dian Zi Cao Hua (Lithospermum erythrorhizon Sieb et Zucc.) (Onosmaconfertum) Lithospermum erythrorhizon (Lithospermum erythrorhizon.) (OnosmaexsertumHemsl.) and the like. However, only Sinkiang arnebia root, Nemeng arnebia root and arnebia root have been collected in the Chinese pharmacopoeia, which is considered as the genuine arnebia root, and other arnebia root including Onosma paniculatum are considered as the counterfeit arnebia root and are not adopted by the traditional Chinese medicine.
The Tibetan radix Arnebiae is Lithospermum erythrorhizon (Boraginaceae) of Yunnan BoraginaceaeOnosmahookeri ClarkeVar. Longiforum Duthie) and Lithospermum erythrorhizon (Onoasmahookeri C．B．Clarke), distributed in eastern and southern parts of the tibet[2]. It is of the same family and different genus as Xinjiang alkanna tinctoria. Although not adopted in traditional Chinese medicine, the history of medication in Tibetan regions in China is long, and according to incomplete statistics, 38 formulas of Tibetan medicine are added with Tibetan lithospermum, which belongs to Tibetan medicine materials with frequent use. Tibetan Lithospermum erythrorhizon (Tibetan medicine name Zhemo), recorded in the Tibetan medicine classic "the four medical classics" of 8 th century of the public Yuan, has sweet, slightly bitter and cool taste in the four five flavors of the Tibetan medicine theory, and recorded in the book Jingzhu Bencao (Kouku Bencao) the blue colored glaze (mannan materia Mingming) and the like, can clear heat and cool blood, nourish lung and treat lung diseases, and is commonly used for pneumonia, cavitary tuberculosis and high mountain hyperemia.
In recent years, along with the development and application of lithospermum medicinal materials, the resources of lithospermum erythrorhizon and lithospermum rimonae are deficient, and the search for lithospermum substitutes is urgent. The onosma paniculatum is the main source of Tibetan lithospermum, the activity and application of the onosma paniculatum only stay in Tibetan medicine formulas, and no modern pharmacological report exists, and no report exists in the aspects of hemostasis and wound healing promotion. The application lays a theoretical foundation for enlarging the lithospermum medicinal source and expanding the medicinal range of the Tibetan lithospermum.
Disclosure of Invention
The invention discloses a preparation method of a Tibetan lithospermum extract for stopping bleeding and promoting wound healing and application thereof in stopping bleeding and promoting wound healing, aiming at solving the problems in the background art.
The technical scheme provided by the invention is as follows:
an active extract of Tibetan lithospermum is characterized in that the active extract is selected from Tibetan lithospermum ethanol extract.
The preferred technical scheme of the invention is as follows:
smashing Tibetan lithospermum into powder, carrying out ultrasonic treatment for 30min by using 0-95% ethanol in a feed-liquid ratio (1: 10-1: 40) (m/v), and filtering; repeating the above steps on the filter residue once, combining the two filtrates, filtering, separating, concentrating with a rotary evaporator at 60 deg.C to obtain ethanol extract, and freeze drying to obtain dry extract.
The ethanol used in the process of the invention is preferably 60-90% ethanol; the material-liquid ratio is preferably 1: 20-1: 30.
the invention also provides a pharmaceutical composition, which comprises the extract prepared by any one of the methods and a pharmaceutically acceptable carrier.
The composition optionally exists in a suitable pharmaceutical preparation form, wherein the preparation form is selected from tablets, capsules, oral liquid, mixture, buccal agent, granules, electuary, pills, powder, ointment, pellet, suspension, solution, injection, powder injection, freeze-dried powder injection, suppository, ointment, plaster, cream, spray, film spray, dripping pills and/or patches; the tablet is preferably sugar-coated tablet, film-coated tablet, enteric-coated tablet or sustained-release tablet; the capsule is preferably a hard capsule, a soft capsule or a sustained release capsule.
The invention further provides application of the Tibetan lithospermum extract in preparing a medicine for stopping bleeding and promoting wound healing. For the experiments related to mouse hemostasis and wound healing, bleeding time, blood coagulation time, platelet count, wound healing rate, hydroxyproline content in wounds and the like are taken as activity indexes. The experimental result shows that the ethanol extract of the Tibetan lithospermum has better hemostatic effect and wound healing promoting effect, has better effect than Xinjiang lithospermum, and can be used for preparing the medicine for stopping bleeding and promoting wound healing.
Description of the drawings:
FIG. 1: the ethanol extract of the Yunnan arnebia euchroma has hemostatic effect;
FIG. 2: the lithospermum erythrorhizon ethanol extract has blood coagulation effect;
FIG. 3: the influence of ethanol extraction of onosma paniculatum on the level of platelets;
FIG. 4: ethanol extraction of onosma paniculatum for wound healing;
FIG. 5: the healing rate of the wound surfaces of each group;
FIG. 6: a comparative graph of the healing rate of the wounds of the onosma paniculatum with different dosages;
FIG. 7: the hydroxyproline content of wound surface of each group.
Detailed Description
Example 1 study of hemostatic action of ethanol extract of Tibetan Lithospermum erythrorhizon
1. Preparation of test samples
1.1 preparation of two ethanol extracts of Lithospermum erythrorhizon
Crushing arnebia euchroma (Royle) Johnst and Onosma paniculatum (Royle) Johnst into powder (sieving with No. 3 sieve, drying at 60 deg.C for 3 hr). Respectively taking quantitative powder, performing ultrasonic treatment with 70% ethanol at a feed-liquid ratio of 1:20 (m/v) for 25min (ultrasonic power of 1800w), and filtering; repeating the above steps on the filter residue once, combining the two filtrates, filtering, separating, concentrating with a rotary evaporator at 60 deg.C to obtain ethanol extract, and freeze drying to obtain dry extract.
1.2 preparation of the liquid medicine
0.5% CMC-Na and Yunnan white drug powder (0.8 g.kg) are respectively prepared by using 0.9% normal saline as solvent-1) Proper amount of Yunnan alkanna tinctoria and 0.5 percent of CMC-Na as solvent to prepare high dose (20g kg)-1) Middle dose (10g kg)-1) And low dose (5 g.kg)-1) And high dose (20g kg) of Onosma paniculatum-1) Middle dose (10g kg)-1) And low dose (5 g.kg)-1) Appropriate amounts of the three concentrations of suspension were used.
2. Grouping of laboratory animals
SPF-level Kunming mice, with a body mass of 18-22 g, half in each case, were provided by WUDUDOU GYOU. Feeding the feed freely, and feeding the feed at room temperature of 18-25 ℃.
Mice with fasting overnight, free drinking water were weighed in a round-robin fashion and 60 mice were selected with a weight difference of no more than 20% of the average body weight. The screened mice are grouped according to a weight random grouping mode, the mice with the same weight are numbered from small to large, the mice with the same weight are put together and coded with the same number, the animals are marked by 80 percent saturated picric acid alcohol solution, then the mice are arranged in each group in sequence, after one group is distributed, the animals are distributed from the last animal in a Z shape. Finally, the mice were divided into 6 groups, which were a blank group, a high-dose group of Onosma paniculatum, a medium-dose group of Onosma paniculatum, a low-dose group of Onosma paniculatum, a positive control 1 group (medium dose of Sinkiang arnebia euchroma), and a positive control 2 group (Yunnan white drug powder), each group containing 10 mice.
3. Research on influence of onosma paniculatum ethanol extract on bleeding time of mice
The influence of the alcohol extract of onosma paniculatum on the bleeding time of mice is researched by adopting a tail breaking method. Each group of mice was administered 1 time daily by gavage for 14 days. 60min after the last administration, fixing the mouse, exposing the mouse to the outside, cutting off the mouse tail tip at a position of 0.5cm by using an operation blade, starting timing when the blood overflows automatically, and sucking off the blood drops by using filter paper for 1 time every 30s until the blood flow stops naturally (no blood is sucked by the filter paper), namely the bleeding time. Mice Bleeding Time (BT) was recorded. The rate of shortening of bleeding time was calculated.
4. Research on influence of onosma paniculatum ethanol extract on blood coagulation time of mice
4.1 capillary method
The influence of the onosma paniculatum alcohol extract on the blood coagulation time of mice is researched by adopting a capillary method. Each group was administered by gavage 1 time daily for 14 days. At 60min after the last administration, according to the capillary tube method, a glass capillary tube with an inner diameter of 1mm is inserted into the posterior venous plexus of the inner canthus of the mouse, the depth is about 4-5 mm, the rotation is gentle and the retraction is carried out, the timing is started when blood flows into the glass tube, and the capillary tube is taken out and placed on the table after reaching 5cm blood column. The capillary tube is broken at intervals of 30s by 0.5cm, whether the blood coagulation silk appears at the broken part is observed, and the time from blood sampling of the capillary glass tube to the appearance of the blood coagulation silk is recorded, namely the blood coagulation time.
4.2 slide method
The influence of the onosma paniculatum alcohol extract on the blood coagulation time of mice is researched by adopting a glass slide method. Each group was administered by gavage 1 time daily for 14 days. 60min after the last administration, rapidly removing eyeball with ophthalmologic forceps, dropping 1 drop of blood from both ends of the glass slide respectively, the diameter of the blood drop is about 6mm, immediately timing with stopwatch, slightly lifting 1 time from the edge of the blood drop with a cleaning pin every 30s, and observing whether blood silk is lifted. The time from the beginning of blood collection to the beginning of blood drawing to the end of blood drawing is the blood coagulation time, and the other drop of blood is used for the retest.
5. Research on influence of onosma paniculatum ethanol extract on mouse platelet count
The influence of the onosma paniculatum ethanol extract on the platelet count of mice is researched. Each group was administered by gavage 1 time daily for 14 days. 60min after the last administration, removing the right eyeball of the mouse to draw blood, sucking 20 mul of the blood with a micropipette into 0.38ml of 1 percent ammonium oxalate solution, fully shaking up, sucking a small drop and placing the drop on a blood cell counting plate, standing for 10-15 min, and counting the number of the blood platelets of each mouse under a high power optical microscope.
6. Data processing
Data were processed using SPSS software and analyzed for significance, with P <0.05 considered statistically different and P <0.01 considered very significant, all data expressed as "Mean ± standard deviation" (Mean ± SD).
7. Results and analysis
7.1 Effect of Alcoholic extract of Onosma paniculatum on bleeding time of mice
The effect of ethanol extract of onosma paniculatum on bleeding time in mice is shown in fig. 1. Compared with a blank control group, the bleeding time of each dose group of the Onosma paniculatum is obviously shortened, the bleeding time of the high dose group is equivalent to that of a positive control Yunnan white drug powder, and the bleeding time of the three dose groups of the Onosma paniculatum is obviously shorter than that of the medium dose group of the Sinkiang arnebia euchroma. The Sinkiang arnebia root high-dose group has almost no hemostatic effect, slight poisoning symptoms also appear, and the hemostatic effect of the medium-dose group and the low-dose group is also poor. The results show that the ethanol extract of the onosma paniculatum can obviously shorten the bleeding time of mice, and the hemostatic effect of the ethanol extract on the mice is superior to that of the arnebia euchroma.
7.2 Effect of Alcoholic extract of Onosma paniculatum on blood coagulation time of mice
The mouse blood coagulation time is evaluated by adopting a glass slide method and a capillary tube method, and the result is shown in figure 2. compared with a blank control group, the blood coagulation time can be obviously shortened by adopting the ethanol extract of the onosma paniculatum with different dosages, and the blood coagulation effect is enhanced along with the increase of the dosage. The blood coagulation time of the high-dose group of the Onosma paniculatum is most equivalent to that of the Yunnan white drug group, and the blood coagulation time of the three dose groups is obviously shorter than that of the medium-dose group of the Sinkiang arnebia euchroma. The arnebia euchroma (Royle) Johnst shows no obvious procoagulant effect in different dosages, and especially shows an obvious blood coagulation inhibiting effect in high dosages. Therefore, the ethanol extract of the onosma paniculatum has certain procoagulant effect, and the coagulation effect of the ethanol extract on mice is superior to that of the arnebia euchroma.
7.3 Effect of Onosma paniculatum Ethanol extract on mouse platelet count
The effect of the onosma paniculatum ethanol extract on the mouse platelet count is shown in fig. 3. Compared with a blank control group, the number of the platelets of each dose group of the onosma paniculatum ethanol extract shows different increases, and the effect of increasing the number of the platelets of mice is increased along with the increase of the administration dose. Wherein the high dose group has the same effect as Yunnan Baiyao, and each group is superior to Sinkiang arnebia root group. The high dose group of the Sinkiang arnebia euchroma has no obvious influence on the number of platelets, and the low dose and the medium dose of the Sinkiang arnebia euchroma show certain platelet increasing effect, but the low dose and the medium dose are not the same as the Yunnan arnebia euchroma under the same dose. The result shows that the ethanol extract of the onosma paniculatum can improve the number of the mouse blood platelets and has better effect than that of the Sinkiang arnebia euchroma.
In the above research, the influence of different doses of the onosma paniculatum ethanol extract on the bleeding time, the blood coagulation time and the number of platelets of mice is investigated by taking Yunnan white drug powder and Sinkiang lithospermum as positive controls. The results show that the bleeding time and the blood coagulation time of the mice can be shortened to different degrees by three different dosages of the onosma paniculatum ethanol extract, and the number of the blood platelets of the mice is increased. The three indexes are integrated, and the ethanol extract of the onosma paniculatum has a certain hemostatic effect, the high-dose group effect of the ethanol extract of the onosma paniculatum is equivalent to that of Yunnan white drug powder, and the hemostatic effect of the ethanol extract of the onosma paniculatum is better than that of the ethanol extract of the onosma paniculatum. Therefore, in the aspect of hemostasis, the onosma paniculatum is expected to be a substitute of arnebia euchroma to meet the medicinal requirement, and the research lays a theoretical foundation for expanding the medicinal range of the onosma paniculatum.
Example 2 study of wound healing promoting effect of ethanol extract of Tibetan radix Arnebiae
Test method 1
1.1 preparation of test specimens
Pulverizing radix Arnebiae (radix Lithospermi) and radix Arnebiae (radix Lithospermi) into powder, and sieving (60 mesh) for use. Respectively taking quantitative powder, performing ultrasonic extraction with 75% ethanol at a feed-liquid ratio of 1:20 (m/v), and filtering; repeating the above steps once for the filter residue, and combining the two filtrates. Concentrating at 60 deg.C with rotary evaporator to obtain ethanol extract, sealing, and drying in a dryer.
1.2 grouping
Yunnan Zicao group of longhua:
a1 high dose group: 2g/mL suspension of Onosma paniculatum, the dosage is 0.1mL/10 g;
dose groups in a 2: 1g/mL suspension of onosma paniculatum, and the dosage is 0.1mL/10 g;
a3 low dose group: 0.5g/mL suspension of onosma paniculatum, with the dosage of 0.1mL/10 g;
arnebia euchroma (Royle) Johnst group:
b1 high dose group: 2g/mL suspension of arnebia euchroma (Royle) Johnst, and the dosage is 0.1mL/10 g;
dose group in B2: 1g/mL suspension of Sinkiang arnebia root, and the dosage of 0.1mL/10 g;
b3 low dose group: 0.5g/mL suspension of Sinkiang arnebia root, and the dosage of 0.1mL/10 g;
blank control group C: 0.5 percent of sodium carboxymethyl cellulose, and the dosage is 0.1mL/10 g;
positive control group: 1% Yunnan Baiyao powder suspension, and the dosage is 0.1mL/10 g.
2 establishment of skin injury mouse animal model
SPF-level Kunming mice (Duoduo Biotechnology Co., Ltd.), the body weight of 18-22 g, and the number of the mice is 48.
2.1 preparation of depilatory Agents
Weighing 4.9 g of Na each time2S·9H2And O, putting 15mL of distilled water into a clean 100mL beaker to prepare an 8% sodium sulfide solution, putting the beaker on an electronic balance to zero after the solid is dissolved, and adding 15g of oily hand cream for later use.
Preparation of 2.210% chloral hydrate
2g of chloral hydrate and 20mL of distilled water were added to a 50mL beaker each time and stirred. The injection amount was 0.04mL/10 g.
2.3 depilation
The hair on the back of the mouse was cut off (about 1.5 cm. times.1.5 cm) using a surgical scissors, and a depilatory (8% sodium sulfide solution) was dipped in a glass rod and applied to the depilated area in a thin layer, and after about 1min, the depilatory and the hair were washed off with warm water, and the back of the mouse was wiped with gauze after washing.
2.4 anesthesia
Mice were anesthetized with an intraperitoneal injection of 10% chloral hydrate (0.04 mL/10 g).
2.5 shear Range marking
Drawing a circle with a radius of 0.56cm on a piece of harder paper by compasses, cutting off, placing the circle in the center of the back of a mouse and marking the position deviating from the tail by a marking pen to form a circle with a size of about 1cm2The area of (a).
2.6 preparation of mouse wound model
Drawing a circle with a radius of 0.56cm on a piece of harder paper by compasses, cutting off, placing the circle in the center of the back of a mouse and marking the position deviating from the tail by a marking pen to form a circle with a size of about 1cm2The area of (a).
The anesthetized mouse is subjected to pronation, 75% alcohol is disinfected conventionally, the skin of the back of the mouse is cut to the shallow layer of deep fascia along a marking line by using instruments such as sterile surgical scissors and the like along the circular marking line, the subcutaneous fascia is cut off, and a mouse upper back skin total incisional wound model is established; care was taken to remove the subcutaneous flesh layer completely until the superficial surface of the muscle membrane to prevent wound contraction (surgical scissors and other instruments should be disinfected with alcohol before and after use). The wound surface of the mouse is firstly stopped by gauze and disinfected by alcohol cotton.
3 divided administration
Mice were randomly grouped into groups of 12 mice each. Putting each mouse into a mouse cage, adding standard feed and tap water, naturally illuminating, periodically changing padding materials, cleaning, and keeping the room temperature at 18-23 ℃ for feeding.
Mice of groups a1, B1: smearing the suspension of 2g/mL of Onosma paniculatum and 2g/mL of Sinkiang arnebia euchroma on the wound once per day according to the dose of 0.1mL/10 g;
mice of groups a2, B2: smearing the suspension of Onosma paniculatum and Sinkiang arnebia root at a dose of 0.1mL/10g once per day, wherein the dose of the suspension is 1 g/mL;
mice of groups a3, B3: smearing the suspension of Onosma paniculatum and Sinkiang arnebia root at a dose of 0.1mL/10g once per day with a dose of 0.5g/mL and 0.5 g/mL;
group B mice: the Xinjiang purple suspension (positive control 1 group) with 1g/mL of grass is smeared on the wound once a day according to the dose of 0.1mL/10 g;
group C mice: applying 1% Yunnan Baiyao powder suspension (positive control group 2) to wound once per day at a dose of 0.1mL/10 g;
group D mice: applying to the wound once a day at a dose of 0.1mL/10g with 0.5% sodium carboxymethylcellulose (blank control);
wound application was performed daily for 14 days at the same time as possible. The body weight of the mice before and after molding is recorded, and the changes of the fur, the movement, the feeding and the like of the mice are observed.
4 wound healing conditions
And 3, 7, 11 and 14d after the wound, visually observing the changes of the wound surface, the growth of granulation tissues and the like, describing the size of the wound by using transparent glass paper, transferring the wound to filter paper, shearing the filter paper along the mark, weighing, and calculating the healing rate of the wound surface. Wound healing rate = (initial area of wound-area of non-healing)/initial area of wound × 100%.
5 hydroxyproline assay
All mice in each group are respectively taken, killed by cervical dislocation after anesthesia, wound surface skin tissues are cut, the hydroxyproline kit is used according to an alkaline hydrolysis method operation method, the absorbance value of each group is measured at 560nm by an enzyme-labeling instrument, and the hydroxyproline content of the wound surface of each group of mice is calculated.
6 results of the experiment
6.1 wound healing Condition
The effect of each group of drugs on wound healing in mice is shown in figure 4.
By observing the wound healing of the mice, it was found that the wound area of each group of mice began to decrease at day 3. On day 7, scabbing appeared on the wound surface of mice in the high-dose groups of Onosma paniculatum, Sinkiang Zicao and the positive control group. On day 11, the wound area of each group of mice was significantly reduced compared to day 1, and eschar appeared on the wound surfaces of mice in the medium-dose group of Onosma paniculatum, the low-dose group of Onosma paniculatum, the medium-dose group of Sinkiang arnebia paniculata, the low-dose group of Sinkiang arnebia paniculata, and the blank group. On day 14, the wound surface of the mice in the blank group remained scabbed, and the wounds of the Onosma paniculatum group, the Sinkiang arnebia euchroma group and the positive control group were substantially healed.
6.2 wound healing Rate comparison
The healing rates for the various groups of wounds are shown in figure 5. Compared with the blank group, the wound healing rate of the other groups at day 14 is higher than that of the blank group at day 14, which shows that the Onosma paniculatum and the Sinkiang arnebia euchroma have promotion effect on wound healing; wherein four groups of Yunnan white drug group, Yunnan alkanna tinctoria low dose, Yunnan alkanna tinctoria medium dose and Sinkiang alkanna tinctoria low dose groups have obvious effect of promoting wound healing.
The effect of different doses of Onosma paniculatum on wound healing is shown in figure 6. After 14 days of administration, the wound healing rate of each group at 14 days is higher than that of the blank control group, and the various dose groups, the positive control 1 group and the positive control 2 group of the Onosma paniculatum have promotion effects on wound healing compared with the blank control group. Wherein the positive control group 2, the low-dosage group of the Onosma paniculatum and the medium-dosage group of the Onosma paniculatum have obvious wound healing promoting effect. The wound healing effect of the low-dose group of the Onosma paniculatum is the best in days 3, 7 and 11, and is higher than that of other control groups; the healing effect of the wound surface of the onosma paniculatum middle dose group is close to that of the positive control group 2 in the 3 rd, 7 th and 11 th days, and the healing rate of the 14 th day is higher than that of other dose groups; the healing effect of the wound surface is lowest on days 3, 7 and 11 in the high-dose group of the Onosma paniculatum, but the healing trend is relatively stable, and the healing effect on day 14 is higher than that of the blank group.
6.3 measurement of hydroxyproline content
Hydroxyproline has the capability of promoting wound healing, and researches show that hydroxyproline is mainly concentrated in collagen, participates in the synthesis of collagen and is an essential substance for wound healing. The hydroxyproline content in the wound skin tissues of each group is shown in fig. 7. The results show that the hydroxyproline content of the wound surface of the mice in the Onosma paniculatum group is obviously higher than that of the blank control group and the positive control group 1 (Sinkiang arnebia). Wherein the content of the medium-dose group is the highest and is equivalent to that of the positive control group 2 (Yunnan white drug powder). It can be seen that the related substances in Onosma paniculatum have more excellent effect on wound healing than Sinkiang Lithospermum erythrorhizon.
In the above experiment, it can be known from the observation of the wound healing condition of the mouse that each dose of the onosma floribundum group has the effect of promoting wound healing, but the effect of promoting wound healing is not as good as that of the positive control group 2 (Yunnan white drug powder), wherein the effect of promoting the scab on the wound surface of the mouse is more obvious in the high dose group (2 g/mL) of the onosma floribundum. After the continuous administration for 14 days, the healing rate of the wound surface of each group is higher than that of the blank group, wherein the low dose of the Yunnan puccoon and the promoting effect of the medium dose of the Yunnan puccoon are more obvious than those of other groups.
Proline has important application value in the treatment of various healing-resistant wounds, and can be gathered in the wound area to synthesize more collagen. One of the main components of collagen is hydroxyproline. Therefore, this study determined wound healing by measuring hydroxyproline content in mouse callus. The results show that the hydroxyproline content of each dose group of the onosma paniculatum is obviously higher than that of the blank control group and the positive control group 1 (arnebia euchroma) and the content of the dose group in the onosma paniculatum is the highest and is equivalent to that of the positive control group 2 (yunnan white drug), which shows that the related substances in the onosma paniculatum have better effect on wound healing than that of the arnebia euchroma.
In conclusion, the onosma paniculatum has a promoting effect on wound healing, has a better effect on promoting wound healing than Sinkiang arnebia euchroma, and is expected to become a substitute of Sinkiang arnebia euchroma in the aspect of wound healing.
Claims (8)
1. An active extract of Tibetan lithospermum is characterized in that the active extract is selected from Tibetan lithospermum ethanol extract.
2. The extract as claimed in claim 1, wherein: smashing Tibetan lithospermum into powder, carrying out ultrasonic treatment for 30min by using 0-95% ethanol in a feed-liquid ratio (1: 10-1: 40) (m/v), and filtering; repeating the above steps on the filter residue once, combining the two filtrates, filtering, separating, concentrating with a rotary evaporator at 60 deg.C to obtain ethanol extract, and freeze drying to obtain dry extract.
3. An extract as claimed in claim 2, wherein: the ethanol is preferably 60-80% ethanol, and the ratio of the ethanol to the liquid is preferably 1: 20-1: 30.
4. a pharmaceutical composition comprising the extract of any one of claims 1-3 and a pharmaceutically acceptable carrier.
5. The pharmaceutical composition of claim 4, wherein the composition is in a form suitable for pharmaceutical use, and the form is selected from the group consisting of tablets, capsules, oral liquids, mixtures, buccal formulations, granules, electuaries, pills, powders, ointments, pellets, suspensions, solutions, injections, powder injections, lyophilized powder injections, suppositories, ointments, plasters, creams, sprays, film sprays, dripping pills, and/or patches.
6. Pharmaceutical composition according to claim 5, characterized in that the tablets are selected from sugar-coated tablets, film-coated tablets, enteric-coated tablets or sustained-release tablets; the capsule is selected from hard capsule, soft capsule or sustained release capsule.
7. Use of an extract according to any one of claims 1 to 3 or a composition according to any one of claims 4 to 6 in the manufacture of a medicament for use in hemostasis.
8. Use of an extract as claimed in any one of claims 1 to 3 or a composition as claimed in any one of claims 4 to 6 in the manufacture of a medicament for promoting wound healing.
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| CN103175907A (en) * | 2012-12-05 | 2013-06-26 | 宁夏多维药业有限公司 | Quality detection method for 25-ingredient podophyllum pills |
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| CN108785355A (en) * | 2018-07-20 | 2018-11-13 | 西藏大学 | A kind of extraction process of radix onosmatis extract |
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| CN103175907A (en) * | 2012-12-05 | 2013-06-26 | 宁夏多维药业有限公司 | Quality detection method for 25-ingredient podophyllum pills |
| CN103417781A (en) * | 2013-08-10 | 2013-12-04 | 济南伟传信息技术有限公司 | Traditional Chinese medicine for treating surgical wounds |
| CN108785355A (en) * | 2018-07-20 | 2018-11-13 | 西藏大学 | A kind of extraction process of radix onosmatis extract |
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