CN102357180A - Traditional Chinese medicine composite for curing cancer and preparation method and application thereof - Google Patents
Traditional Chinese medicine composite for curing cancer and preparation method and application thereof Download PDFInfo
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- CN102357180A CN102357180A CN2011103272837A CN201110327283A CN102357180A CN 102357180 A CN102357180 A CN 102357180A CN 2011103272837 A CN2011103272837 A CN 2011103272837A CN 201110327283 A CN201110327283 A CN 201110327283A CN 102357180 A CN102357180 A CN 102357180A
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Abstract
The invention discloses a traditional Chinese medicine composite for curing cancer and a preparation method and application thereof. The composite is substantially prepared by fritillary. The preparation method comprises the following steps: smashing the fritillary into powder; adding water into other traditional Chinese medicine and decocting, filtering decoction, and concentrating filtrate to obtain extract; adding the fritillary powder to the extract, evenly mixing and obtaining the traditional Chinese medicine composite. The traditional Chinese medicine composite for curing the cancer has little toxicity and remarkable curing effect of the cancer.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition and preparation method thereof, particularly a kind of Chinese medicine composition of treating cancer and preparation method thereof.
Background technology
Bulbus Fritillariae Cirrhosae is the dry bulb of liliaceous plant Bulbus Fritillariae Cirrhosae, dark violet Bulbus Fritillariae Uninbracteatae, Gansu Bulbus Fritillariae Uninbracteatae or Bulbus Fritillariae cirrhosae; Have clearing heat and moistening lung, the effect of preventing phlegm from forming and stopping coughing can be used for lung-heat type cough, dry cough few expectorant, deficiency of YIN chronic cough, sputum with blood, controls cough due to consumptive disease, the spitting of blood of spitting, ambition pent-up, consumptive lung disease, lung abscess, sore throat, acute mastitis; It also contains multiple alkaloid modern chemistry research; Like compositions such as fritimine, chinpeimine, beilupeimine, fritiminine, sonpeimine and second, imperialine, imperialine, minpeimine, minpeiminine, chuanbeinones, have antitussive, eliminate the phlegm, antibiotic, antiinflammatory, spasmolytic; Rising blood glucose, the pharmacological action of blood pressure lowering.
The Rhizoma Anemarrhenae is the dry rhizome of the monocotyledon liliaceous plant Rhizoma Anemarrhenae; Has clearing away heat-fire; The effect of promoting the production of body fluid and moisturizing; Can be used for treating corresponding diseases such as epidemic febrile disease, hyperpyrexia excessive thirst, cough and asthma, cough caused by dryness, constipation, osteopyrexia and fever, restlessness of asrhenia type and insomnia, the stranguria with turbid discharge of quenching one's thirst, it mainly contains zhimusaponin A-I, A-II, A-III, A-IV modern study, compositions such as B-I and B-II; Still the Mengiferin, the isomangiferin that contain flavonoid; The choline of alkaloids, niacin amide, compositions such as the tannic acid of organic acid, nicotinic acid and anemaran, modern pharmacology show that it has resisting pathogenic microbes, antibiotic, blood fat reducing, atherosclerosis, analgesic, blood sugar lowering, radiation-resistant effect.
Promptly not seeing so far has Bulbus Fritillariae Cirrhosae and Rhizoma Anemarrhenae compatibility can effectively treat tumor, the report of effective antitumor.
Summary of the invention
The objective of the invention is to disclose a kind of Chinese medicine composition of treating cancer.
The present invention also aims to disclose a kind of method for preparing of treating the Chinese medicine composition of cancer.
The present invention also aims to disclose the application of a kind of Chinese medicine composition in preparation treatment cancer drug.
The present invention also aims to disclose a kind of Chinese medicine composition and suppress the application in the angiogenesis drug in preparation.
The present invention also aims to disclose the application of a kind of Chinese medicine composition in preparation treatment lung-cancer medicament.
The present invention also aims to disclose a kind of Chinese medicine composition and have the application in the treatment liver-cancer medicine in preparation.
The present invention also aims to disclose the application of a kind of pharmaceutical composition in preparation treatment lung-cancer medicament.
The present invention also aims to disclose a kind of pharmaceutical composition and have the application in the treatment liver-cancer medicine in preparation.
The present invention realizes through following technical scheme:
A kind of Chinese medicine composition of treating cancer, its crude drug is mainly formed (by weight) by following Chinese medicine:
Rhizoma Anemarrhenae 1-10 weight portion Bulbus Fritillariae Cirrhosae 2-9 weight portion.
Preferably, said Chinese medicine composition, its crude drug mainly is made up of following Chinese medicine:
Rhizoma Anemarrhenae 3-8 weight portion Bulbus Fritillariae Cirrhosae 4-7 weight portion.
Preferably, said Chinese medicine composition, its crude drug mainly is made up of following Chinese medicine:
The Rhizoma Anemarrhenae 5 weight portion Bulbus Fritillariae Cirrhosaes 5 weight portions.
Preferably, said Chinese medicine composition, its crude drug mainly is made up of following Chinese medicine:
The Rhizoma Anemarrhenae 3 weight portion Bulbus Fritillariae Cirrhosaes 8 weight portions.
Preferably, said Chinese medicine composition, its crude drug mainly is made up of following Chinese medicine:
The Rhizoma Anemarrhenae 7 weight portion Bulbus Fritillariae Cirrhosaes 6 weight portions.
Preferably, said Chinese medicine composition, its crude drug mainly is made up of following Chinese medicine:
The Rhizoma Anemarrhenae 8 weight portion Bulbus Fritillariae Cirrhosaes 4 weight portions.
A kind of method for preparing of treating the Chinese medicine composition of cancer, said method for preparing includes but not limited to following method:
Step 1: get Bulbus Fritillariae Cirrhosae, be ground into powder;
Step 2: get Rhizoma Anemarrhenae decocte with water, decocting liquid filters, and filtrating is concentrated into extractum;
Step 3: get extractum, in extractum, add Bulbus Fritillariae Cirrhosae powder, mixing gets Chinese medicine composition of the present invention;
Preferably, Bulbus Fritillariae Cirrhosae powder is broken into fine powder, more preferably No. 8 sieves;
Preferably, the Rhizoma Anemarrhenae adds 4-8 times of weight water gaging and decocts 2-4 time, and each 20-60 minute, collecting decoction filtered, and gets filtrating;
Preferably, above-mentioned filtrate decompression is concentrated into the extractum of relative density 1.25-1.35 (60 ℃);
The present invention adds pharmaceutically acceptable regular dosage form adjuvant in the Chinese medicine composition of above-mentioned gained; According to the regular dosage form method for preparing; Process the dosage form of clinical acceptance, said dosage form includes but not limited to capsule, tablet, soft capsule, granule, powder, oral liquid formulations, pill, honeyed pill, slow releasing preparation, lyophilized injectable powder etc.
Dosage form according to the invention is preferably granule, can select the conventional granulates method for preparing, preferably is prepared as follows method:
Step 1: get Rhizoma Anemarrhenae 1-10 weight portion, Bulbus Fritillariae Cirrhosae 2-9 weight portion;
Step 2: Bulbus Fritillariae Cirrhosae powder is broken into fine powder;
Step 3: the Rhizoma Anemarrhenae: decocting boils, and filters, and filtrating is concentrated into extractum;
Step 4: add Bulbus Fritillariae Cirrhosae fine powder and appropriate amount of auxiliary materials in step 3 extractum, mixing is granulated, and drying promptly gets.
Adjuvant in the step 4 is selected from soluble starch, dextrin, lactose and Icing Sugar etc.; Preferred lactose or dextrin.
The method that decocting boils in the method for preparing of above-mentioned granule is preferably: the Rhizoma Anemarrhenae adds 6 times of water gagings and decocts 3 times, each 30min, collecting decoction;
Simmer down to concentrating under reduced pressure in the step 3 in the method for preparing of above-mentioned granule;
In the method for preparing of above-mentioned granule in the step 3 filtrate decompression be concentrated into the extractum of relative density 1.35 (60 ℃);
Add 65% alcohol granulation in the method for preparing of above-mentioned granule.
The crude drug of pharmaceutical composition of the present invention is that the Rhizoma Anemarrhenae, Bulbus Fritillariae Cirrhosae are formed; Through evidence mice S180 sarcoma is had the obvious suppression effect, Lewis lung cancer is had the effect of obvious suppression tumor tumor bulk-growth, the lung of Lewis lung cancer is shifted number also has significant inhibitory effect; Mice H22 tumor had good tumor-inhibiting action; Strain has the obvious suppression effect to human primary liver cancer's cell QGY-7703 tumor, so can suppress tumor growth, cancer is had therapeutical effect; And Chinese medicine composition of the present invention also can suppress angiogenesis, suppresses the basis of tumor continued growth, the nutrition supply of blocking-up tumor cell; Cause tumor no longer to continue to grow up, downright bad until tumor cell, lump dwindles and even disappears; Reach the purpose of treatment cancer, the present invention is a natural Chinese medicines also, and toxicity is little; Not only can eliminate tumor cell and can also protect normal cell, can increase immunocyte and cytoactive, improve curative effect.
Bulbus Fritillariae Cirrhosae belongs to expensive thin medical material class in the pharmaceutical composition of the present invention; Tradition promptly is used as medicine to beat powder; Anemarrhena ratio of rhizome medicinal material, effective ingredient are mainly anemaran and timosaponin class, take all factors into consideration traditional medication custom and day clothes dosage; The clinical needs of the disease of treating, the advantage that preferential selection has decoction is carried again, the granule of taking convenience is a preferred dosage form.
The present invention further also provides a kind of pharmaceutical composition of treating cancer, and its active component composition and proportioning are following:
1-timosaponin A-1-III 0.5-2 weight portion, chimonin 0.5-2 weight portion, Peimine 2-7 weight portion, Peiminine 3-8 weight portion.
The pharmaceutical composition of said treatment tumor is preferably:
1-timosaponin A-1-III: chimonin: Peimine: Peiminine=1: 1: 4: 5.5.
1-timosaponin A-1-III, chimonin, Peimine and Peiminine all can be bought through the conventional method preparation or through market and obtain.
The present invention adds pharmaceutically acceptable regular dosage form adjuvant in above-mentioned described pharmaceutical composition; According to the regular dosage form method for preparing; Process the dosage form of clinical acceptance, said dosage form includes but not limited to capsule, tablet, soft capsule, granule, powder, oral liquid formulations, pill, honeyed pill, slow releasing preparation, lyophilized injectable powder etc.
For above-mentioned dosage form can be realized, need when these dosage forms of preparation, to add the pharmacy acceptable auxiliary, for example: filler, disintegrating agent, lubricant, suspending agent, binding agent, sweeting agent, correctives, antiseptic, substrate etc.Filler comprises: starch, pregelatinized Starch, lactose, mannitol, chitin, microcrystalline Cellulose, sucrose etc.; Disintegrating agent comprises: starch, pregelatinized Starch, microcrystalline Cellulose, carboxymethyl starch sodium, crospolyvinylpyrrolidone, low-substituted hydroxypropyl cellulose, cross-linking sodium carboxymethyl cellulose etc.; Lubricant comprises: magnesium stearate, sodium lauryl sulphate, Pulvis Talci, silicon dioxide etc.; Suspending agent comprises: polyvinylpyrrolidone, microcrystalline Cellulose, sucrose, agar, hydroxypropyl emthylcellulose etc.; Binding agent comprises, starch slurry, polyvinylpyrrolidone, hydroxypropyl emthylcellulose etc.; Sweeting agent comprises: saccharin sodium, Aspartane, sucrose, cyclamate, enoxolone etc.; Correctives comprises: sweeting agent and various essence; Antiseptic comprises: parabens, benzoic acid, sodium benzoate, sorbic acid and its esters, benzalkonium bromide, fixed, the Folium eucalypti globueli (Eucalyptus globulus Labill.) wet goods of acetic acid chloroethene; Substrate comprises: PEG6000, PEG4000, insect wax etc.
Experimental example 1 Study on Preparation
1.1 crude drug source
The Bulbus Fritillariae Cirrhosae medical material: purchase that lotus pond Chinese crude drug Xiao of specialized wholesale market medical herbs Chinese medicine is capable in Chengdu, professor Wang Shu is accredited as Bulbus Fritillariae Cirrhosae through HuaXi college of pharmacy, SiChuan University, derives from Bulbus Fritillariae Cirrhosae Fritillaria cirrhosa D.Don.
The Rhizoma Anemarrhenae (life) decoction pieces: purchase new Flos Nelumbinis decoction pieces company of the good group of state, lot number: 1002021,1003006,1009047,1008161 in Chengdu.
1.2 the investigation of Rhizoma Anemarrhenae decoction pieces extraction process by water
1.2.1 the investigation of Rhizoma Anemarrhenae decoction pieces water absorption rate
Get Rhizoma Anemarrhenae decoction pieces 50g, 3 parts, respectively add water 300ml, the every observation at a distance from 30min once soaked into the mood condition, all soaks into until medical material, leaches whole unabsorbed water liquid, and the volume of measuring filtrating calculates its water absorption rate and is about 180%.
1.2.2 the investigation of Rhizoma Anemarrhenae decoction pieces extraction process
The principal element that decocting is carried is solvent consumption, infiltrating time, extraction time and extraction time etc., and each factor is selected three levels, presses L9 (34) orthogonal table arrangement test.Rhizoma Anemarrhenae decoction pieces 30g is got in every part of test, amounts to 9 parts and carries out reflux, extract.Because timosaponin B II is its main effective ingredient in the Rhizoma Anemarrhenae; So with timosaponin B II content, dried cream recovery rate is that index is carried out comprehensive grading; According to practical situation and with reference to ratio commonly used the weight coefficient of timosaponin B II content and dried cream recovery rate is decided to be 0.6,0.4 respectively; Thereby filter out optimum extraction process, the factor level of orthogonal test is seen table 1.
Table 1 orthogonal test factor level
The mensuration of timosaponin B II is with reference to " the assay condition under a P197 page or leaf of Chinese pharmacopoeia version in 2010 the rhizoma ane marrhenae item is measured.
Chromatographic condition and system suitability: Tianjin, island LC-10AT high performance liquid chromatograph; C18 WelchromTM HPLC column (4.6*250mm, 5 μ l) type chromatographic column is a mobile phase with acetonitrile-water (25: 75); Low form evaporative light scattering detector (SEDEX 75LT-ELSD) detects; 30 ℃ of column temperatures, drift tube temperature are made as 40 ℃, and number of theoretical plate calculates by timosaponin B II peak should be not less than 10000.
The processing of sample: take by weighing Rhizoma Anemarrhenae decoction pieces 30g, make an experiment by orthogonal table, extracting solution is settled to 1000ml, and precision is measured 50ml; When water-bath was waved to the also surplus about 25ml of liquid, the about 10g of adding kieselguhr stirred; Baking does not have excessive moisture when stirring in 70 ℃ of baking ovens, and surperficial substantially dry, and kieselguhr all is transferred in the tool plug conical flask; Add 30% acetone 100ml, supersound process 30min filters; Filtrating is settled to 100ml, crosses 0.45 μ m microporous filter membrane, supplies the assay of timosaponin B II to use.In addition precision is measured dilution back Rhizoma Anemarrhenae extracting solution 25ml, places in the evaporating dish of constant weight, in water-bath, dries, and baking is 3 hours in 105 ℃ of baking ovens, takes out, and places in the exsiccator, and the cooling back is claimed to decide weight, calculates dried cream recovery rate.
Result of the test is seen table 2,3.
1.2.2.1 orthogonal design and result that the Rhizoma Anemarrhenae extracts
Table 2 orthogonal experiment plan is taken into account the result
The analysis of table 3 orthogonal experiments
The result shows: influence factor D＞C＞A＞B, wherein factor D has significance to the influence of extracting.Consider the requirement of production cost and industrialized great production, so confirm that the extraction process condition is A
1B
1C
1D
3Be the water that Rhizoma Anemarrhenae decoction pieces adds 6 times of amounts, reflux, extract, 3 times, each 30min.
1.2.2.2 the checking of Rhizoma Anemarrhenae decoction pieces extraction process
In order to verify the accuracy of The above results,,, arrange repeated trials, result of the test such as table 4 by above-mentioned definite process conditions A1B1C1D3 to guarantee the rationally feasible of extraction process.
The confirmatory experiment of table 4 Rhizoma Anemarrhenae extraction process
Demonstration test result shows that the extraction process of the Rhizoma Anemarrhenae is stable, has repeatability and operability.
1.2.3 the investigation of Rhizoma Anemarrhenae extracting solution concentration technology
Through the production status in Literature Consult and present pharmaceutical factory, consider destruction and the influence of temperature to the timosaponin constituents, so adopt the mode of concentrating under reduced pressure to concentrate Rhizoma Anemarrhenae extracting solution, spissated temperature is about 70 ℃, vacuum is-0.07～-0.1Mpa.
1.4 the investigation of moulding process of the present invention
1.4.1 the screening of Tendrilled Fritillaria Bulb granularity
The preferred granule preparation technology design according to the present invention, Bulbus Fritillariae Cirrhosae is used as medicine for beating powder, so prepared granule belongs to the suspension ability granule.According to the preparation requirement of suspension ability granule, the powder size of Bulbus Fritillariae Cirrhosae is investigated, in conjunction with the trial test result; According to Tendrilled Fritillaria Bulb 6g, Rhizoma Anemarrhenae extractum (1.35,60 ℃ of relative densities) 4g; The proportioning of dextrin 4g is granulated; With reference to " an appendix IC of Chinese pharmacopoeia version in 2010 granular preparation general rule is carried out the investigation of melting, result such as table 5 to the granule that makes.
The investigation result of table 5 Tendrilled Fritillaria Bulb granularity
Result of the test shows, Bulbus Fritillariae Cirrhosae is crossed granule that the powder of No. eight sieves (150 mesh sieve) processes in the melting checking process, and the suspendible situation is more excellent, does not have macroscopic deposition basically, so final to select the Bulbus Fritillariae Cirrhosae granularity of being used as medicine be the powder of No. eight sieves (150 mesh sieve).
1.4.2 single factor of Rhizoma Anemarrhenae extractum relative density is investigated
Adopt the mode of concentrating under reduced pressure, Rhizoma Anemarrhenae extracting solution is concentrated into relative density 1.1 (60 ℃), place again and continue in the water-bath to concentrate; In conjunction with the trial test result, according to Tendrilled Fritillaria Bulb 6g, Rhizoma Anemarrhenae extractum (different relative densities; 60 ℃; Gross weight/rhizoma ane marrhenae amount * 6 of extractum after the certain relative density of the consumption of Rhizoma Anemarrhenae extractum=be concentrated into), the proportioning of dextrin 4g is granulated.Investigate relative density 1.20 (60 ℃), 1.25 (60 ℃), 1.30 (60 ℃), 1.35 (60 ℃), five relative densities of 1.40 (60 ℃) are to the influence of molding situation, result such as table 6.
The single factor of table 6 Rhizoma Anemarrhenae extractum relative density is investigated the result
Result of the test shows that Rhizoma Anemarrhenae extractum relative density 1.20 (60 ℃) is rarer, difficult forming; 1.40 (60 ℃) are too thick, molding effect is also not good, and relative density that can molding is at 1.25～1.35 (60 ℃).
The influence experiment of 2 pairs of S180 tumors of experimental example
1 experiment material
1.1 medicine
According to the granule of the present invention of embodiment 1 preparation, be made into the suspension of variable concentrations during use with normal saline.
Fluorouracil (5-FU) injection: Tianjin gold credit aminoacid company limited is produced lot number 0503271.
Physiological saline solution: Sichuan Kelun Large Pharmaceutical Factory Co. Ltd, lot number B050502-07.
1.2 laboratory animal
Kunming mouse, body weight 18-22g, single sex.Provided by institute of antibiotics, Sichuan Province Experimental Animal Center, meet healthy one-level animal, animal produces the quality certification: the real kinoplaszm in river 99-31 number.
1.3 tumor cell
Murine sarcoma S180 cell is provided by the Huaxi Medical Univ institute of oncology.Go down to posterity liquid nitrogen cryopreservation through the mouse peritoneal inoculation.
2 experimental techniques
Get frozen S180 cell, cell suspension is processed with normal saline in the recovery back, and platform is expected blue repelling attack living cell counting number, by 1～2 * 10
6Cell inoculation is in mouse peritoneal, and after about 7～10 days, obvious ascites appears in mice.Get the mice of above-mentioned abdominal cavity inoculated tumour cell, the sterilization abdominal part, aseptic extraction peritoneal fluid, 1000 left the heart 5 minutes, supernatant discarded, normal saline washes twice, and platform is expected blue repelling attack living cell counting number.Be made into 1 * 10 with normal saline
7Cell/ml cell suspension, right side of mice oxter subcutaneous injection 0.2ml/ are only.Mouse hypodermic inoculation second day is divided into 5 groups with it by body weight at random, and the high, normal, basic dose groups of the present invention is pressed 4.1g/kg respectively, 2.1g/kg, and 1.05g/kg irritates the suspension of the present invention that stomach gives respective concentration.Every day 1 time, continuous 10 days; Fluorouracil (5-FU) group is pressed 20mg/kg dosage lumbar injection, every day 1 time, continuous 7 days.Matched group was irritated the normal saline that stomach gives equal volume in continuous 10 days.After the last administration second day, separate tumor mass, weigh, calculate tumour inhibiting rate.
Adopt the SPSS10.0 statistical software that tumor is heavily carried out one factor analysis of variance (ONEWAY ANOVA), and select for use two tail Dunnett t checks comparing between each drug group and the matched group.
3 experimental results
Table 7 the present invention is to the influence of mice S180 sarcoma
* P＜0.001 vs normal saline group
As shown in table 7, each dosage of the present invention all has the better inhibited effect to murine sarcoma S180, wherein, and the tumour inhibiting rate of low dose group even surpassed conventional chemotherapy medicine 5-FU.
4 conclusions: the present invention has the obvious suppression effect to mice S180 sarcoma.
The research of 3 pairs of chick chorioallantoic membrane angiogenesiss of experimental example inhibitory action
1 material and instrument
The injection normal saline; Distilled water; Hatching egg (strong chicken farm, Chengdu); Microscope (Leica company); Neutral filter paper; Chinese medicine composition of the present invention (embodiment 3 preparations).
2 experimental techniques
2.1 Embryo Gallus domesticus is hatched
Hatching egg is earlier wiped surface blot, reuse 75% alcohol disinfecting with the 1 ‰ new gills liquid that goes out.Hatching egg is answered major part (air chamber end) up, is 45 with the egg holder, hatches in the electric heating incubator, and temperature is put a water pond and kept relative humidity at 40%-70% at 37.8 ℃ in the incubator, stay a passage, to guarantee the supply of oxygen.Hatch in the process turning egg(s) 4 times every day, prevent embryo's adhesion, promote the amniotic membrane motion.
2.2CAM model preparation
2.2.1 window
Hatched the 7th day, the about 2cm in cotton ball soaked in alcohol sterilization egg air chamber (stub end) surface * 3cm zone of 75%, reuse dental wheel mill is cut eggshell.After waiting to grind an indenture, carefully press from both sides shell breaking and shell membrane with the curved tweezer of ophthalmology.Window size is by the decision of air chamber size, generally about 0.5cm apart from the air chamber base.
2.2.2 expose CAM
After windowing, on cameral mantle, drip a droplet normal saline with disposable syringe earlier, when treating that physiology saline slowly spreads apart; The CAM blood vessel that is close to below the cameral mantle just displays; And separate with the cameral mantle on upper strata and to lower recess, carefully push the cameral mantle here and provoke with syringe needle this moment at the CAM that drips normal saline place lower floor, the curved tweezer of the hand-held ophthalmology of another is taken advantage of a situation the cameral mantle of provoking is clamped; Clamp; Under the assistance of syringe needle, gently cameral mantle is little by little thrown off simultaneously, CAM is exposed fully.Be sure not to damage CAM when preparing false air chamber, influence the survival of Embryo Gallus domesticus in order to avoid cause microvascular bleeding.
2.2.3 dosing
After exposing CAM, prior ready carrier is placed the rare district of CAM central vessel, so that medicine to be measured plays one's part to the full.Each group drips medicine 10 μ l with micro sample adding appliance respectively, uses the false air chamber of adhesive tape sealing label then, forms transparent windows, observes the survival of Embryo Gallus domesticus.Envelope window continued is hatched, and the envelope window must be tight, and the whole operation process should guarantee aseptic as far as possible.
2.4.4 make the CAM BIAO and BEN
3d is hatched in dosing, cuts off adhesive tape, adds methanol by observation window: the fixative 5-10 of acetone=1: 1 drips; Pre-fix 15-20min; After the blood vessel of treating CAM solidifies fully, remove chorion and membrana putaminis more than the CAM plane carefully with the curved tweezer of ophthalmology, and use eye scissors intactly to cut CAM as the center with determinand; Be placed in the plate that fills normal saline, be tiled on the wave carrier piece after trembling exhibition with tweezers.HE dyeing, light microscopic observe down CAM blood vessel structure and the inner new vessels of the growing state of observing tumor cell, morphological change and tumor area distribution, quantity and with the relation of host blood vessel on every side.
2.5 vascular counts
Count new capillary vessel with 1 * 10 times under the anatomic microscope; Interior with experiment edge, position (being the microporous filter membrane carrier edge) 1mm scope is the one-level blood vessel; 5mm place, edge, position is the secondary blood vessel with experiment; The blood vessel of all genus tropisms growth promptly is that send at the center with the carrier, with the angle of filter membrane radius less than 45 °.The person all gives counting, and the blood vessel of walking, detouring is then not very interior.The I and II blood vessel is observed counting respectively.
2.6 medication preparation
Get Chinese medicine composition of the present invention (embodiment 3 preparation) 5g, be mixed with disposable aspiration needle formula filter filtration sterilization after 7mg crude drug in whole/ml concentration with physiological saline solution before the experiment, packing, 4 ℃ of refrigerators are preserved subsequent use.Face time spent reuse normal saline and under aseptic condition, dilute concentration for response.With the qualitative filter paper is sample carrier, processes the scraps of paper of 5mm diameter with card punch, and sterilization cooling back is arranged in filter paper on the aseptic aluminium foil in super-clean bench, adds 150 μ l test solution altogether respectively, and the blank group only adds the distilled water that dilutes usefulness.Filter paper sample is all air-dry in super-clean bench.
3 experimental results
3.1 chick chorioallantoic membrane blood vessel area value and area ratio measurement
3.1.1 morphological observation: blank control group CAM film and carrier plate merge, and the part trunk runs through.The big and small vessel less light speckle of distribution relatively all appears in basic, normal, high dose groups.In the low dose group, the intensive phenomenon of dish Zhou Youxiao blood vessel, only there are the minority minute blood vessel in middle dosage and high dose group dish week; With the point sample place is that the center is and holds or radial distribution; Blood vessel is grown seldom to carrier, and vessel branch obviously reduces, and does not radially distribute; Angiogenesis obviously receives to press down, and each organizes Embryo Gallus domesticus gray value result such as Fig. 1-shown in Figure 7.
3.1.2 blood vessel area value and area ratio measurement, the result sees table 8
Table 8 is respectively organized Embryo Gallus domesticus blood vessel area value/area ratio relatively
Annotate: compare * * P＜0.01 o'clock with blank control group
Conclusion: can be found out that by table 8 blank control group blood vessel area value and area ratio are lower, and this ratio of each dose groups of the present invention increases significantly than blank control group all, and significant statistical significance (P＜0.01) is arranged, comparing with the positive drug thalidomide does not have statistical significance.Prompting the present invention has significant inhibitory effect to the chick chorioallantoic membrane angiogenesis, and demonstrates certain dose-effect relationship trend.
The influence experiment that test of 4 couples of mice lung cancer Lewis of experimental example tumor inhibition effect and lung thereof shift
1 experiment material
1.1 medicine
The present invention is by according to embodiment 1 method preparation, is made into the suspension of variable concentrations during use with normal saline.
Fluorouracil (5-FU) injection: Tianjin gold credit aminoacid company limited is produced lot number 0503271.
Physiological saline solution: Sichuan Kelun Large Pharmaceutical Factory Co. Ltd, lot number B030502-07.
1.2 laboratory animal
The C57BL/6 mice, body weight 18-22g, female, purchase Experimental Animal Center in Shanghai, meet healthy one-level animal.
1.3 tumor cell:
Mice Bearing Lewis Lung Cancer is provided by the Huaxi Medical Univ institute of oncology.With C57BL/6 mice interior generation, liquid nitrogen cryopreservation.
2 experimental techniques
Get lotus tumor Lewis lung cancer mice, the aseptic tumor mass of peeling off is cut into small pieces with little shears, grinds to form cell suspension with normal saline on the net at 100 order stainless steel sifts, and C57BL/6 right side of mice oxter subcutaneous injection 0.2ml/ only.Inoculate back second day, animal is divided into 5 groups at random by body weight, the high, normal, basic dose groups of the present invention is pressed 4.2g/kg respectively, 2.1g/kg, 1.05g/kg primary crude drug gastric infusion.Every day 1 time, continuous 14 days; Fluorouracil (5-FU) group is pressed 20mg/kg dosage lumbar injection, every other day once, and continuous 7 times.Matched group was irritated the normal saline that stomach gives equal volume in continuous 12 days.After the last administration second day, separate tumor mass, weigh, calculate tumour inhibiting rate; Separate lungs simultaneously, weigh, the formed tuberosity number that counting tumor lung shifts
Adopt the SPSS10.0 statistical software that tumor is heavily carried out one factor analysis of variance (ONEWAY ANOVA), and select for use two tail Dunnett t checks comparing between each drug group and the matched group.
3 experimental results
Experimental result shows that successive administration is after 12 days, and the present invention is high, in, low dose group has the obvious suppression effect to Mice Bearing Lewis Lung Cancer, and tumour inhibiting rate is 31.8%, 34.9%, 45.7%, has compared significant difference with the normal saline matched group.Simultaneously, it is heavy that each dosage of the present invention all can reduce lung, and obviously reduce the transfer number of tumor at lungs.With the normal saline matched group significant difference is arranged.
Table 9 the present invention is to the influence of Mice Bearing Lewis Lung Cancer and lung transfer thereof
* P＜0.05 vs normal saline group, * * P＜0.01 vs normal saline group
4 conclusions
The present invention is at high dose, and middle dosage and low dosage have the effect of obvious suppression tumor tumor bulk-growth to Lewis lung cancer.In each dose groups the lung of Lewis lung cancer is shifted number significant inhibitory effect is also arranged.
Experimental example 5 the present invention are to the tumor suppression rate of growth of rat liver cancer H22 and the influence experiment of transfer
1 experiment material
1.1 medicine
The present invention is by according to embodiment 1 method preparation, is made into the suspension of variable concentrations during use with normal saline.
Fluorouracil (5-FU) injection: Tianjin gold credit aminoacid company limited is produced lot number 0503271.
Physiological saline solution: Sichuan Kelun Large Pharmaceutical Factory Co. Ltd, lot number B060502-07.
1.2 laboratory animal
Kunming mouse, body weight 18-22g, single sex.Provided by institute of antibiotics, Sichuan Province Experimental Animal Center, meet healthy one-level animal, animal produces the quality certification: the real kinoplaszm in river 99-31 number.
1.3 tumor cell
Rat liver cancer ascitic type H22 tumor cell is provided by the Huaxi Medical Univ institute of oncology.Go down to posterity liquid nitrogen cryopreservation through the mouse peritoneal inoculation.
2 experimental techniques
Get frozen H22 cell, cell suspension is processed with normal saline in the recovery back, and platform is expected blue repelling attack living cell counting number, by 1～2 * 10
6Cell inoculation is in mouse peritoneal, and after about 7～10 days, obvious ascites appears in mice.Get the mice of above-mentioned abdominal cavity inoculated tumour cell, the sterilization abdominal part, aseptic extraction peritoneal fluid, 1000 left the heart 5 minutes, supernatant discarded, normal saline washes twice, and platform is expected blue repelling attack living cell counting number.Be made into 1 * 10 with normal saline
7Cell/ml cell suspension, right side of mice oxter subcutaneous injection 0.2ml/ are only.Mouse hypodermic inoculation second day is divided into 5 groups with it by body weight at random, and the high, normal, basic dose groups of the present invention is pressed 4.1g/kg respectively, 2.1g/kg, and the 1.05g/kg primary crude drug is irritated the solution of the present invention that stomach gives respective concentration.Every day 1 time, continuous 10 days; Fluorouracil (5-FU) group is pressed 20mg/kg dosage lumbar injection, every day 1 time, continuous 7 days.Matched group was irritated the normal saline that stomach gives equal volume in continuous 10 days.After the last administration second day, separate tumor mass, weigh, calculate tumour inhibiting rate;
Adopt SPSS 10.0 statistical softwares that tumor is heavily carried out one factor analysis of variance (ONEWAY ANOVA), and select for use two tail Dunnett t checks comparing between each drug group and the matched group.
3 experimental results
As shown in table 10; The present invention has significant inhibitory effect 1.8g/kg and 0.9g/kg dosage successive administration 10 days to mice H22 tumor, and its tumour inhibiting rate is more than 40%; 3.6g/kg the group tumour inhibiting rate also reaches 36%, each administration group tumor weight is compared significant difference with the normal saline matched group.
Table 10 the present invention is to the influence of mice H22 tumor
* P＜0.05 vs normal saline group, * * P＜0.01 vs normal saline group
4 conclusions
The present invention has good tumor-inhibiting action to mice H22 tumor in vivo
Experimental example 6 the present invention are to human lung cancer cell A549 and primary hepatocarcinoma cell SMMC-7721 tumor strain body outer suppressioning experiment
1 instrument, equipment and equipment
1.1 cell
A549 is human lung carcinoma cell, available from the Shanghai cell bank; SMMC-7721 is human primary liver cancer's cell, purchases in the Huaxi Medical Univ institute of oncology, with RPMI-1640 complete medium (RPMI-1640+10% calf serum), at 5%CO
2, 37 ℃ of cultivations of going down to posterity.
1.2 main agents and preparation thereof
RPMI-1640, trypsin are the GIBCO product; MTT, trypan blue are the Sigma product; DMSO, the Long Huagongshijichang of Chengdu section.
1.3 key instrument
Electronic analytical balance (100,000/): Sai Duolisi BP-210D
Low speed centrifuge: Beijing Medical Centrifugal Machine Factory.
BIORAD 550 type enzymes join detector
BB5060 CO2 incubator: German HERAEUS.
Inverted microscope: Japanese OLMPUS product.
Cell counting count board: Shanghai medical optical instrument factory.
96 porocyte culture plates, 50ml centrifuge tube, Tissue Culture Flask are the FALCON product.
2 test samples and reference substance
Test sample: Chinese medicine composition of the present invention (embodiment 2 preparations), Peimine, Peiminine, chimonin and 1-timosaponin A-1-III (being standard substance) available from Sichuan Province medicine inspecting institute.Above-mentioned substance all is set as 500g/ml, 250 μ g/ml, 125 μ g/ml, 62.5 μ g/ml, 31.25 μ g/ml, 15.63 μ g/ml, 7 concentration group of 7.81 μ g/ml, and medicine is made into 500 μ g/ml concentration with the RPMI-1640 complete medium; 0.22 the filtration sterilization of μ m membrane filter is diluted to respective concentration with the RPMI-1640 complete medium during experiment.
Reference substance: cisplatin injection: lot number: 060301, authentication code: the accurate word H53021740 of traditional Chinese medicines, produced by Gejiu Bio-Pharmaceutical Co., Ltd., Yunnan.50 μ g/ml, 25 μ g/ml, 12.5 μ g/ml, 6.25 μ g/ml, 3.125 μ g/ml, 1.5625 μ g/ml, 0.78125 a μ g/ml7 concentration are established in experiment, are diluted to respective concentration with complete medium during experiment.
3 experimental techniques
Cell harvesting: the cultivation of going down to posterity of tumor cell routine, merge and discarded culture fluid to 80% o'clock, add 0.25% pancreatin+0.02%EDTA digestion, when treating that cell rounding becomes single, add complete medium, adherent cell is beaten in featheriness, makes it form single cell suspension.Collecting cell is to centrifuge tube.1000 rev/mins centrifugal 5 minutes.Supernatant discarded adds culture medium.The trypan blue repelling attack is carried out cell counting, adjustment cell concentration to 5 * 10
4Cell/ml.Get 96 orifice plates, every hole adds cell suspension 200 μ l with pipettor, and making every porocyte number is 1.0 * 10
4Individual.The blank group only adds culture fluid and does not add cell, puts 37 ℃, 5%CO
2Cultivate 24h in the incubator.
Take out 96 orifice plates of cultivating 24h, the careful suction of pipettor goes supernatant, every hole to add the drug solution 200 μ l with the complete medium preparation respectively, and each concentration is established three multiple holes.Blank adds the complete medium of 200 μ l, and positive control adds cisplatin 200 μ l.Put 37 ℃, 5%CO
2Cultivate in the incubator.
Add and take out 96 orifice plates after medicinal liquid is cultivated 24h, examine under a microscope the cell growth condition record earlier, careful then the suction removed supernatant, and every hole adds the complete medium of 180 μ l and the MTT (5mg/ml) of 20 μ l, 37 ℃, 5%CO with liquid-transfering gun
2Incubator continues to hatch 4h, inhales and removes supernatant, and every hole adds dimethyl sulfoxide 150 μ l, the 10min that fully vibrates dissolving, and ELIASA 490nm place measures the OD value.The OD value that records all should deduct the OD value of blank.
4. investigation index
Light microscopic is observed the influence of drug effect 24h, 48h cell growth situation down.Investigate the drug level value of T/C=50%.Be IC
50Value (growth inhibited)
4. date processing
Date processing is with the T/C value representation, T/C%=100% * processed group OD value/matched group OD value, and suppression ratio (%)=(1-processed group OD value/matched group OD value) * 100% through regression Calculation, is obtained the drug level of T/C=50%.
5. experimental result
Table 11 the present invention is to the influence of 2 kinds of tumor cell in-vitro multiplications
To A549, the present composition, chimonin, 1-timosaponin A-1-III, 1-timosaponin A-1-III: chimonin (1: 1) and 1-timosaponin A-1-III: chimonin: Peimine: (1: 1: 4: 5.5) inhibitory action was all very obvious, IC for Peiminine
50Be respectively 7.94 μ g/ml, 19 μ g/ml, 17 μ g/ml, 12.96 μ g/ml and 9.05 μ g/ml; To SMMC-7721, the present composition, 1-timosaponin A-1-III and 1-timosaponin A-1-III: chimonin: Peimine: (1: 1: 4: 5.5) inhibitory action was all very obvious, IC for Peiminine
50Be respectively 11.75 μ g/ml, 25.94 μ g/ml and 13.82 μ g/ml.
Experimental example 7 different medicine groups are to the influence of test of mice lung cancer Lewis tumor inhibition effect and lung transfer thereof
1 experiment material
1.1 medicine
Bulbus Fritillariae Cirrhosae group: be broken into fine powder (crossing sieve No. 8);
Bulbus Fritillariae Thunbergii group: be broken into fine powder (crossing sieve No. 8);
The Radix Scutellariae group: add 6 times of water gagings and decoct 3 times, each 30min, collecting decoction filters, and filtrate decompression is concentrated into the thick paste of relative density 1.35 (60 ℃);
Rhizoma Anemarrhenae group: add 6 times of water gagings and decoct 3 times, each 30min, collecting decoction filters, and filtrate decompression is concentrated into the thick paste of relative density 1.35 (60 ℃);
Bulbus Fritillariae Cirrhosae+Rhizoma Anemarrhenae group: the method preparation of pressing embodiment 1
Bulbus Fritillariae Thunbergii+Rhizoma Anemarrhenae group (1: 1): Bulbus Fritillariae Thunbergii is ground into fine powder (crossing sieve No. 8), and Rhizoma Anemarrhenae decoction pieces adds 6 times of water gagings and decocts 3 times, each 30min, and collecting decoction filters, and filtrate decompression is concentrated into the thick paste of relative density 1.35 (60 ℃), adds the Bulbus Fritillariae Thunbergii fine powder;
Radix Scutellariae+Rhizoma Anemarrhenae group (1: 1): add 6 times of water gagings and decoct 3 times, each 30min, collecting decoction filters, and filtrate decompression is concentrated into the thick paste of relative density 1.35 (60 ℃);
Be made into the suspension of variable concentrations during use with normal saline.
Fluorouracil (5-FU) injection: Tianjin gold credit aminoacid company limited is produced lot number 0503271.
Physiological saline solution: Sichuan Kelun Large Pharmaceutical Factory Co. Ltd, lot number B030502-07.
1.2 laboratory animal
The C57BL/6 mice, body weight 18-22g, female, purchase Experimental Animal Center in Shanghai, meet healthy one-level animal.
1.3 tumor cell:
Mice Bearing Lewis Lung Cancer is provided by the Huaxi Medical Univ institute of oncology.With C57BL/6 mice interior generation, liquid nitrogen cryopreservation.
2 experimental techniques
Get lotus tumor Lewis lung cancer mice, the aseptic tumor mass of peeling off is cut into small pieces with little shears, grinds to form cell suspension with normal saline on the net at 100 order stainless steel sifts, and C57BL/6 right side of mice oxter subcutaneous injection 0.2ml/ only.Inoculate back second day; Animal is divided into 9 groups at random by body weight, i.e. normal saline group, 5 fluorouracil groups, Bulbus Fritillariae Cirrhosae group, Bulbus Fritillariae Thunbergii group, Radix Scutellariae group, Rhizoma Anemarrhenae group, Bulbus Fritillariae Cirrhosae+Rhizoma Anemarrhenae group, Bulbus Fritillariae Thunbergii+Rhizoma Anemarrhenae group, the Radix Astragali+Rhizoma Anemarrhenae group, every animals administer is 2.1g crude drug in whole/kg; The administration volume is a 10ml/kg primary crude drug gastric infusion; The normal saline group gives isopyknic normal saline, every day 1 time, continuous 14 days; 5 fluorouracil (5-FU) group is pressed 20mg/kg dosage lumbar injection, every other day once, and continuous 7 times.After the last administration second day, separate tumor mass, weigh, calculate tumour inhibiting rate; Separate lungs simultaneously, the formed tuberosity number that counting tumor lung shifts.
Adopt the SPSS10.0 statistical software that tumor is heavily carried out one factor analysis of variance (ONEWAY ANOVA), and select for use two tail Dunnett t checks comparing between each drug group and the matched group.
3 experimental results
See table 12, experimental result shows that successive administration is after 14 days, and two female granules are high, in, low dose group has the obvious suppression effect to Mice Bearing Lewis Lung Cancer, and tumour inhibiting rate is 31.8%34.9%, 45.7%, compared significant difference with the normal saline matched group.Simultaneously, it is heavy that two each dosage of female granule all can reduce lung, and obviously reduce the transfer number of tumor at lungs.With the normal saline matched group significant difference is arranged.
The combination of table 12 different pharmaceutical is to the influence of Mice Bearing Lewis Lung Cancer and lung transfer thereof
Compare * P＜0.05 with the normal saline group; * P＜0.01; * * P＜0.001
Compare with Bulbus Fritillariae Cirrhosae+Rhizoma Anemarrhenae group,
△P＜0.05;
△ △P＜0.01;
△ △ △P＜0.001
4 conclusions
The effect that Bulbus Fritillariae Cirrhosae+Rhizoma Anemarrhenae compatibility has obvious suppression tumor tumor bulk-growth and lung to shift to the Lewis lung cancer model, the two compatibility has synergism.And tumor growth and metastasis inhibition are run business into strong one and significantly are superior to Bulbus Fritillariae Thunbergii+Rhizoma Anemarrhenae and Radix Scutellariae+Rhizoma Anemarrhenae.
Description of drawings
Fig. 1: negative control (blank) Embryo Gallus domesticus gray value;
Fig. 2: positive control (thalidomide) Embryo Gallus domesticus gray value;
Fig. 3: I group Embryo Gallus domesticus gray value of the present invention;
Fig. 4: II group Embryo Gallus domesticus gray value of the present invention;
Fig. 5: III group Embryo Gallus domesticus gray value of the present invention;
Fig. 6: IV group Embryo Gallus domesticus gray value of the present invention;
Fig. 7: V group Embryo Gallus domesticus gray value of the present invention;
The specific embodiment
Following embodiment all can realize technique effect of the present invention.
Bulbus Fritillariae Cirrhosae 500g Rhizoma Anemarrhenae 500g
More than two flavors, Bulbus Fritillariae Cirrhosae powder is broken into fine powder (crossing sieve No. 8), Rhizoma Anemarrhenae decoction pieces adds 6 times of water gagings and decocts 3 times; Each 30min, collecting decoction filters; Filtrate decompression is concentrated into the thick paste of relative density 1.35 (60 ℃), adds Bulbus Fritillariae Cirrhosae fine powder and 292g dextrin, mixing; Process granule after adding 65% ethanol adjustment soft material humidity, drying promptly gets granule.
Embodiment 2 tablets
Bulbus Fritillariae Cirrhosae 500g Rhizoma Anemarrhenae 300g
More than two flavors, Bulbus Fritillariae Cirrhosae powder is broken into fine powder (crossing sieve No. 8), Rhizoma Anemarrhenae decoction pieces adds 7 times of water gagings and decocts 3 times; Each 40min, collecting decoction filters; Filtrate decompression is concentrated into the thick paste of relative density 1.34 (60 ℃), adds the Bulbus Fritillariae Cirrhosae fine powder, mixing; Add the conventional adjuvant of tablet, promptly get tablet according to the tablet routine fashion.
Embodiment 3 capsules
Bulbus Fritillariae Cirrhosae 400g Rhizoma Anemarrhenae 700g
More than two flavors, Bulbus Fritillariae Cirrhosae powder is broken into fine powder (crossing sieve No. 8), Rhizoma Anemarrhenae decoction pieces adds 6 times of water gagings and decocts 4 times; Each 30min, collecting decoction filters; Filtrate decompression is concentrated into the thick paste of relative density 1.31 (60 ℃), adds the Bulbus Fritillariae Cirrhosae fine powder, mixing; Add the conventional adjuvant of capsule,, promptly get capsule according to the capsule routine fashion.
Embodiment 4 pills
Bulbus Fritillariae Cirrhosae 500g Rhizoma Anemarrhenae 750g
More than two flavors, Bulbus Fritillariae Cirrhosae powder is broken into fine powder (crossing sieve No. 8), Rhizoma Anemarrhenae decoction pieces adds 8 times of water gagings and decocts 2 times; Each 60min, collecting decoction filters; Filtrate decompression is concentrated into the thick paste of relative density 1.28 (60 ℃), adds the Bulbus Fritillariae Cirrhosae fine powder, mixing; Add the conventional adjuvant of pill,, promptly get pill according to the pill routine fashion.
Embodiment 5 oral liquids
Bulbus Fritillariae Cirrhosae 200g Rhizoma Anemarrhenae 800g
More than two flavors, Bulbus Fritillariae Cirrhosae powder is broken into fine powder (crossing sieve No. 8), Rhizoma Anemarrhenae decoction pieces adds 6 times of water gagings and decocts 2 times; Each 50min, collecting decoction filters; Filtrate decompression is concentrated into the thick paste of relative density 1.32 (60 ℃), adds the Bulbus Fritillariae Cirrhosae fine powder, mixing; Add the conventional adjuvant of oral liquid,, promptly get oral liquid formulation according to the oral liquid routine fashion.
Embodiment 6 granules
Bulbus Fritillariae Cirrhosae 800g Rhizoma Anemarrhenae 400g
More than two flavors, Bulbus Fritillariae Cirrhosae powder is broken into fine powder (crossing sieve No. 8), Rhizoma Anemarrhenae decoction pieces adds 6 times of water gagings and decocts 3 times; Each 30min, collecting decoction filters; Filtrate decompression is concentrated into the thick paste of relative density 1.35 (60 ℃), adds Bulbus Fritillariae Cirrhosae fine powder and dextrin 360g, mixing; Process granule after adding 65% ethanol adjustment soft material humidity, drying promptly gets granule.
Embodiment 7 granules
Bulbus Fritillariae Cirrhosae 500g Rhizoma Anemarrhenae 200g
More than two flavors, Bulbus Fritillariae Cirrhosae powder is broken into fine powder (crossing sieve No. 8), Rhizoma Anemarrhenae decoction pieces adds 6 times of water gagings and decocts 3 times; Each 30min, collecting decoction filters; Filtrate decompression is concentrated into the thick paste of relative density 1.35 (60 ℃), adds Bulbus Fritillariae Cirrhosae fine powder and dextrin 210g, mixing; Process granule after adding 65% ethanol adjustment soft material humidity, drying promptly gets granule.
Embodiment 8 tablets
1-timosaponin A-1-III 1g, chimonin 1g, Peimine 4g, Peiminine 5.5g;
Adopt common process to process tablet.
Claims (10)
1. Chinese medicine composition of treating cancer is characterized in that crude drug mainly is made up of following Chinese medicine:
Rhizoma Anemarrhenae 1-10 weight portion Bulbus Fritillariae Cirrhosae 2-9 weight portion.
2. Chinese medicine composition as claimed in claim 1 is characterized in that crude drug mainly is made up of following Chinese medicine:
Rhizoma Anemarrhenae 3-8 weight portion Bulbus Fritillariae Cirrhosae 4-7 weight portion.
3. Chinese medicine composition as claimed in claim 1 is characterized in that crude drug mainly is made up of following Chinese medicine:
4. pharmaceutical composition of treating cancer is characterized in that crude drug mainly is made up of following medicine:
1-timosaponin A-1-III 0.5-2 weight portion chimonin 0.5-2 weight portion
Peimine 2-7 weight portion Peiminine 3-8 weight portion.
5. like the method for preparing of the arbitrary described Chinese medicine composition of claim 1-3, it is characterized in that this method for preparing comprises the steps:
Step a: get Bulbus Fritillariae Cirrhosae, be ground into powder;
Step b: get Rhizoma Anemarrhenae decocte with water, decocting liquid filters, and filtrating is concentrated into extractum;
Step c: get extractum, in extractum, add Bulbus Fritillariae Cirrhosae powder, mixing adds the pharmacy adjuvant and processes the dosage form of clinical acceptance.
6. the method for preparing of Chinese medicine composition as claimed in claim 5 is characterized in that this method for preparing comprises the steps:
Step a: Bulbus Fritillariae Cirrhosae powder is broken into fine powder;
Step b: get the Rhizoma Anemarrhenae and add 4-8 times of weight water gaging decoction 2-4 time, each 20-60 minute, collecting decoction filtered, and filtrate decompression is concentrated into 60 ℃ of extractum of surveying relative density 1.25-1.35;
Step c: get extractum, in extractum, add Bulbus Fritillariae Cirrhosae powder, mixing adds the pharmacy adjuvant and processes the dosage form of clinical acceptance.
7. like the application of the arbitrary described Chinese medicine composition of claim 13 in preparation treatment lung-cancer medicament.
8. like the application of the arbitrary described Chinese medicine composition of claim 1-3 in preparation treatment liver-cancer medicine.
9. the application of pharmaceutical composition as claimed in claim 4 in preparation treatment lung-cancer medicament.
10. the application of pharmaceutical composition as claimed in claim 4 in preparation treatment liver-cancer medicine.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103599122A (en) * | 2013-11-29 | 2014-02-26 | 沈阳药科大学 | Application of timosaponin AIII in anemarrhena to preparation of antitumor drugs |
| CN106110182A (en) * | 2016-06-30 | 2016-11-16 | 杨亚清 | A kind of Chinese medicine composition treating cancer |
| CN107684599A (en) * | 2016-08-05 | 2018-02-13 | 广州白云山潘高寿药业股份有限公司 | Application of the Chinese medicine composition in the medicine for preparing treatment adenocarcinoma of lung |
| CN107982295A (en) * | 2014-08-20 | 2018-05-04 | 漳州片仔癀药业股份有限公司 | The application of Pien Tze Huang and its preparation in medicine for anti transfer of tumor is prepared |
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| CN1086723A (en) * | 1993-06-09 | 1994-05-18 | 吴传福 | The manufacture method of compound pulmonary carcinoma tablet |
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| CN1086723A (en) * | 1993-06-09 | 1994-05-18 | 吴传福 | The manufacture method of compound pulmonary carcinoma tablet |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103599122A (en) * | 2013-11-29 | 2014-02-26 | 沈阳药科大学 | Application of timosaponin AIII in anemarrhena to preparation of antitumor drugs |
| CN107982295A (en) * | 2014-08-20 | 2018-05-04 | 漳州片仔癀药业股份有限公司 | The application of Pien Tze Huang and its preparation in medicine for anti transfer of tumor is prepared |
| CN106110182A (en) * | 2016-06-30 | 2016-11-16 | 杨亚清 | A kind of Chinese medicine composition treating cancer |
| CN107684599A (en) * | 2016-08-05 | 2018-02-13 | 广州白云山潘高寿药业股份有限公司 | Application of the Chinese medicine composition in the medicine for preparing treatment adenocarcinoma of lung |
| CN107684599B (en) * | 2016-08-05 | 2020-05-08 | 广州白云山潘高寿药业股份有限公司 | Application of traditional Chinese medicine composition in preparation of medicine for treating lung adenocarcinoma |
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Effective date of registration: 20190203 Address after: Fourth Floor, Women's and Children's Activity Center, Changdu West Road, Tibet Autonomous Region, 854000 Patentee after: Enway Pharmaceutical Co., Ltd. Address before: 610041 No. 18 Gaopeng East Road, Chengdu High-tech Zone, Sichuan Province Patentee before: Chengdu Enwei Investement (Group) Co., Ltd. |