CN114588185A

CN114588185A - Pet gel with functions of regulating intestines and stomach and protecting oral cavity and preparation method thereof

Info

Publication number: CN114588185A
Application number: CN202210152681.8A
Authority: CN
Inventors: 王军军
Original assignee: Shenzhen Hongrui Biotechnology Co ltd
Current assignee: Shenzhen Hongrui Biotechnology Co ltd
Priority date: 2022-02-18
Filing date: 2022-02-18
Publication date: 2022-06-07
Also published as: CN116173081A

Abstract

The invention provides a pet gel with functions of regulating intestines and stomach and protecting oral health and a preparation method thereof, wherein the pet gel comprises the following raw materials: inulin, xylo-oligosaccharide, erythritol, dipotassium glycyrrhizinate, herbal extracts, glycerol, sodium alginate, vitamin C, anhydrous citric acid, sodium benzoate and purified water. The pet gel with the functions of intestine and stomach conditioning and oral cavity health care can simultaneously improve two ozostomia problems of oral source type ozostomia and intestine and stomach type ozostomia, has stronger oral cavity health care effect and stronger intestine and stomach conditioning effect, uses the efficacy of herbal extracts, and does not add chemical antibacterial agents and antibiotics.

Description

Pet gel with functions of regulating intestines and stomach and protecting oral health and preparation method thereof

Technical Field

The invention relates to the technical field of pet intestine and stomach conditioning, in particular to pet gel with intestine and stomach conditioning and oral health care functions and a preparation method thereof.

Background

In recent years, cats are increasingly popular with modern people as pets, and are quiet, independent, clean and the like, so that the cats are very suitable for being raised by modern people. In life, cats are not only emotional partners but also important members of the family, and their physical and mental health conditions also draw the mood of the pet owner. With the improvement of living standard of people, pet raising modes and methods are gradually standardized, including purchasing pet food, snacks and toys; inoculating a vaccine; treatment of disease; but subtle problems missing in life often become the cause of illness, such as bad breath in cats.

Bad breath in cats is divided into two cases: oral and gastrointestinal. Oral malodor is generally caused by oral problems, and oral malodor is typically caused by gingivitis or calculus. Calculus is generally caused by accumulation of calculus due to long-term non-cleaning of teeth, food with excessive water intake easily causes food residue to adhere to teeth, plaque and calculus are formed along with development of oral bacteria, and odor is generated. While the true etiology of these diseases is currently unknown in relation to gingivitis, it has been reported that multiple etiologies exist alone or in combination to cause inflammation, with possible pathogens including: inflammatory reactions of bacterial, viral, bartonella infection, or alterations in the immune status (feline leukemia virus/feline immunodeficiency virus). Gastrointestinal type oral malodor is generally caused by halitosis due to gastrointestinal diseases, and unhealthy diet can cause gastrointestinal diseases. Two of them are most typical: the indigestion is caused by overeating greasy food; secondly, the stomach and intestine discomfort caused by eating uncooked and cold food is caused. At present, pet mouth wash products for improving the common halitosis problem in the market can only solve the halitosis of oral origin, and have no way to solve the halitosis of intestines and stomach, and the effect is single. At present, the conventional treatment mode for the gastrointestinal halitosis problem of cats generally comprises western medicine treatment and auxiliary conditioning of prebiotics such as oligosaccharide and the like. But western medicines bring side effects to cats, and cats can be affected by certain medicines; meanwhile, western medicines can only play a temporary role in treatment and cannot achieve the effects of long-term regulation and health care. While the auxiliary conditioning of prebiotics only aims at the disturbance of intestinal flora, and has slow effect and long regulation period.

Disclosure of Invention

In order to solve the problems, the invention provides a pet gel with functions of gastrointestinal conditioning and oral health care and a preparation method thereof.

The invention is realized by the following technical scheme:

the invention provides a pet gel with functions of regulating intestines and stomach and protecting oral health, which consists of the following raw materials in percentage by weight: inulin 0.5-3%, xylo-oligosaccharide 0.1-4%, erythritol 0.5-4%, dipotassium glycyrrhizinate 0.01-2%, herbal extract 0.11-12%, glycerol 1-5%, sodium alginate 0.05-0.5%, vitamin C0.02-0.05%, anhydrous citric acid 0.1-0.5%, sodium benzoate 0.01-0.1%, and the balance of purified water.

Preferably, the herbal extract is one or more of an extract of podocarpus macrophyllus, an extract of coreopsis tinctoria, an extract of dandelion, an extract of honeysuckle flower petals and an extract of houttuynia cordata.

Preferably, the herbal extract comprises 0.1-7% of podocarpus macrophyllus extract and 0.01-5% of coreopsis tinctoria petals in percentage by weight.

The invention also provides a preparation method of the pet gel with the functions of regulating intestines and stomach and protecting oral health, which comprises the following steps:

s1, sequentially adding xylo-oligosaccharide, inulin, erythritol and dipotassium glycyrrhizinate into purified water, and carrying out homogenizing stirring for 10min until the materials are completely dissolved, wherein the homogenizing stirring speed is 450r/min, so as to obtain a solution A;

s2, adding anhydrous citric acid, vitamin C and sodium benzoate into the solution A, homogenizing and stirring for 10min to obtain a solution AB, wherein the homogenizing and stirring speed is 400 r/min;

s3, adding the podocarpus macrophyllus extract, sodium alginate and glycerol into the solution AB, homogenizing and stirring for 15min to obtain a solution ABC, wherein the homogenizing and stirring speed is 500 r/min;

s4, adding coreopsis tinctoria petals into the solution ABC, and carrying out homogeneous stirring for 15min to obtain pet dew, wherein the homogeneous stirring speed is 500 r/min.

The invention has the beneficial effects that:

1. the pet gel with the functions of regulating intestines and stomach and protecting oral health can simultaneously improve two ozostomia problems of mouth source type ozostomia and stomach type ozostomia, the product contains effective components such as dipotassium glycyrrhizinate, Kunlun snow chrysanthemum, Japanese cypress extract and the like, has antibacterial and anti-inflammatory effects, volatile oil rich in Kunlun snow chrysanthemum has inhibition effects on cryptococcus biomass and germ tube germination, and the Japanese cypress extract has certain functions of deodorizing and cleaning oral cavity and has stronger treatment effect on the mouth source type ozostomia of cats.

2. The pet gel with the functions of regulating the intestines and stomach and oral health can promote the gastrointestinal peristalsis and regulate the gastrointestinal function, can solve the problem of gastrointestinal halitosis while regulating the gastrointestinal function, has rich dietary fibers to exert better digestion promoting effect, promotes the increase of water drinking amount of cats, further promotes the increase of water in the gastrointestinal tract, and is more beneficial to the discharge of excrement; after the pet gel is used, the abundance of the gastrointestinal flora of the cat is increased, the strain distribution is more uniform, and meanwhile, the dominant strain stabilization pet gel has a positive effect on the abundance of the intestinal microflora of the cat and can maintain the stability of the healthy gastrointestinal flora of the cat.

Drawings

FIG. 1 is a DR image display of 13 cats tested at day 0, such as squirrel, charpy, tippy, dudu, tiger, girl, Blue, chirpy, curry, dahua, fox, scarlet, and dugil;

FIG. 2 is a DR image display of 13 cats tested at day 60, such as squirrel, charpy, tippy, dudu, tiger, girl, Blue, chirpy, curry, dahua, fox, scarlet, and dugil;

FIG. 3 is a graph showing a comparison of longest diameter of abdominal cavity (DR length) of 13 cats selected from squirrel, queen, Dudu, little tiger, girl, Blue, chirpy, curry, dahua, fox, scarlet, and beggar;

figure 4 is a graph of the average water intake of the first three weeks of 30 cats.

Detailed Description

In order to more clearly and completely describe the technical scheme of the invention, the invention is further described with reference to the accompanying drawings.

Firstly, preparation

Example 1

The formula comprises the following components: inulin 0.5%, xylo-oligosaccharide 0.5%, erythritol 1%, dipotassium glycyrrhizinate 0.02%, podocarpus macrophyllus extract 1.2%, coreopsis tinctoria flower petal 0.05%, glycerin 2%, sodium alginate 0.05%, vitamin C0.02%, anhydrous citric acid 0.1%, sodium benzoate 0.01%, and purified water 94.55%.

The preparation method comprises the following steps:

s3, adding the podocarpus macrophyllus extract, sodium alginate and glycerol into the solution AB, and homogenizing and stirring for 15min to obtain a solution ABC, wherein the homogenizing and stirring speed is 500 r/min;

s4, adding Kunlun snow chrysanthemum petals into the solution ABC, and carrying out homogeneous stirring for 15min to obtain pet dew, wherein the homogeneous stirring speed is 500 r/min.

Example 2

The formula comprises the following components: inulin 1.0%, xylo-oligosaccharide 2.0%, erythritol 1.5%, dipotassium glycyrrhizinate 0.05%, podocarpus macrophyllus extract 2.5%, Kunlun snow chrysanthemum flower petal 0.15%, glycerin 3%, sodium alginate 0.06%, vitamin C0.03%, anhydrous citric acid 0.2%, sodium benzoate 0.01% and purified water 89.5%.

The preparation method comprises the following steps:

s1, sequentially adding xylo-oligosaccharide, inulin, erythritol and dipotassium glycyrrhizinate into purified water, and homogenizing and stirring for 10min until the xylo-oligosaccharide, inulin, erythritol and dipotassium glycyrrhizinate are completely dissolved, wherein the homogenizing and stirring speed is 450r/min, so as to obtain a solution A;

Example 3

The formula comprises the following components: inulin 1.5%, xylo-oligosaccharide 3.0%, erythritol 2.0%, dipotassium glycyrrhizinate 0.1%, podocarpus macrophyllus extract 3.5%, coreopsis tinctoria flower petal 1%, glycerol 3%, sodium alginate 0.05%, vitamin C0.05%, anhydrous citric acid 0.3%, sodium benzoate 0.01%, and purified water 85.49%.

The preparation method comprises the following steps:

Second, evaluation test of curative effect

1. Test animal

Before the official test, 30 test animals were selected according to the following criteria: the insect expelling and immunity are well done, the health condition is good, no obvious brain, heart, liver, lung, kidney and blood diseases exist, no long-term medicine taking history exists, the matched animals are not in special stages such as a vaccination or insect expelling period, a pregnancy period, a lactation period, disease prevention and treatment or recovery period and the like, and the animals are raised in the same environment for more than one month. Each cat is fed in a single cage, an independent activity space is provided, and daily disinfection and cleaning of the environment are well done, so that the cat can eat the feed freely.

2. Grouping

TABLE 1 test animals

3. Test method

(1) And (3) measuring normal indexes: continuously measuring the water intake for 3 days, and taking an average value as the normal water intake of the cat; collecting blood, measuring blood routine and biochemical indexes; and performing DR shooting to evaluate gastrointestinal tract images.

(2) Pre-experiment: the pet gel was diluted in daily drinking water (4ml pet gel, 200ml clear water) at a ratio of 1:50, daily drinking water was recorded and clinical performance was observed.

(3) And (3) formal test: after the suitability test was performed, a formal test was started. Diluting the pet gel in drinking water every day (4ml of pet gel and 200ml of clear water) according to a ratio of 1:50, observing and recording the reaction of the cat when the drinking water is changed, and recording the water intake of the cat every day (24 hours) (3 weeks before the formal experiment); clinical manifestations were observed daily during the 2 months of the trial period; collecting feces and detecting intestinal flora on 30 th and 60 th days after the test; and (4) performing weight measurement, blood sampling and blood routine measurement, biochemical index and DR shooting on days 0 and 60, and performing gastrointestinal tract image examination.

(4) And recording abnormal water intake conditions in the test process, such as overturning of a basin, mixing of feed or cat litter and the like.

(5) The test period amounted to 2 months (possibly extended depending on the particular situation), with 3 days for normal water intake, 1 week for pilot or adaptive tests and 8 weeks for formal tests. The pet gel prepared in example 1 was used in the test.

4. Test results

4.1 changes in oral hygiene conditions during the test

The test was conducted by observing the oral cavity of the test cats, which were examined and recorded according to established evaluation criteria, mainly for halitosis and gingivitis at

day

10, 17, 24, 31, 38, 45, 52, and 59 of the test. Evaluation criteria for halitosis: the halitosis evaluation standard adopts a sensory analysis method, the nasal smell method evaluation standard is a 0-5-point system evaluation standard, and when the halitosis is evaluated by the nasal smell method, a full-mouth smell method is adopted. Evaluation criteria for gingivitis: (1) none: and scoring 0. (2) Mild: the gum is slightly reddish and scored 1. (3) Medium: it was difficult to grasp food, the pain of chewing food and the red swelling around the gum were obvious, and 2 points were recorded. (4) And (3) severe degree: anorexia, pain, marked saliva increase, red swelling of the gums and tongue and palate, even ulceration and hyperplasia, progressing to stomatitis, score 3. Table 2 below is a table of bad breath scores for 30 cats and table 3 is a table of gingivitis scores for 30 cats.

TABLE 2 bad breath scoring table

TABLE 3 gingivitis scoring Table

4.2 changes in digestive function during the test

On day 0 of the start of the official experiment, 18 random abdominal DR examinations were performed on test cat populations, 13 (72.22%) of which had significant intestinal content. Scoring for digestive dysfunction by 3 points (3 points: obvious gastrointestinal tract digestive function disorder, abnormal increase of abdominal circumference, and fecal discharge disorder; 2 points: weak gastrointestinal tract digestive function, slightly increased abdominal circumference, and mild constipation; 1 point: weak gastrointestinal tract digestive function, no obvious physical sign disorder; and 0 point: normal gastrointestinal tract digestive function). DR images of experiment day 0 show that the mouse, chart, tip and beep are 3 points; little tiger, quality girl, Blue, wom 2 minutes; curry, Dahua, fox, Dahong and Bidens suis are rated 1, and the rest 5 are rated 0. FIG. 1 is a DR image of 13 cats tested at day 0, such as squirrel, chartlet, Dudu, little tiger, girl, Blue, chirpy, curry, dahua, fox, scarlet, and dugill.

Through 60 days pet dew feed back, to little squirrel, picture, the tip, the blet, little tiger, quality girl, Blue, chirp, curry, big flower, fox, mahong, this 13 cats of little ghost carry out belly DR inspection again, there are 7 intestinal content contents obvious reduction in 13 cats, this 7 cats that obviously change are little ghost of courage, picture, chirp, the tip, the blet, little tiger, quality girl. FIG. 2 is a DR image display of 13 cats tested at day 60, such as squirrel, charming, tippy, Dudu, little tiger, girl, Blue, chirpy, curry, dahua, fox, scarlet and nautilus.

Finally, the DR images of the 13 cats with improved gastrointestinal tract digestion function at day 0 and day 60 show that the longest diameter of the abdominal cavity is compared, and the abdominal circumference is obviously reduced. As shown in fig. 3, a graphical representation of the longest diameter of the abdominal cavity (DR length) is compared. As shown in Table 4, a comparison table of the longest diameter of the abdominal cavity (DR length) was used.

TABLE 4 comparison table

4.3 Change in composition of enteric bacteria during the test

Randomly selecting 10 test cats, collecting and storing feces on 30 th and 60 th days, and then detecting and sequencing, wherein the first sampling number of the 10 cats is A1-10, the second sampling number is B1-10, and the numbers of the first sampling number and the second sampling number correspond to the feces of the same cat. The sequencing results after the examination are shown in Table 5. Clear Reads is a high-quality Reads number obtained after the quality control of the original sequence; effective Reads is the Effective sequence number of the Clean Reads after splicing (double ends), filtering length and chimera; avglen (bp) is the average sequence length of the sample; GC (%) is the GC content of the sample, i.e.the percentage of bases of the G and C type in the total; q20 (%) is the percentage of bases having a mass value of 20 or more based on the total number of bases; q30 (%) is the percentage of bases having a mass value of 30 or more based on the total number of bases; effect (%) is the percentage of Effect Reads to Raw Reads.

TABLE 5 results of sample sequencing treatment

The characteristic sequences are subjected to taxonomic annotation by using a naive Bayes classifier by taking SILVA as a reference database to obtain species classification information corresponding to each characteristic, so that the community composition of each sample is counted at each level (phylum, class, order, family, genus, speces), and a species abundance table (Table 6) at different classification levels is generated by using QIIME software. In this sample, 12 phyla, 18 classes, 38 orders, 68 families, 166 genera and 185 species were involved.

TABLE 6 statistical table of species of each grade of sample

4.4 Water intake variation during the test

The water intake test adopts the change of the water intake of the cats three weeks before the formal test for analysis and comparison.

During the test, the water intake of 30 cats in the formal test is recorded to obtain the daily average feed intake change curve, as shown in fig. 4, it can be found that: the data of part of days have larger difference and are possibly related to the temperature change and the overturn of a basin in the same day, the daily water intake of the cats after the addition of the pet condensate is generally in an ascending trend and is more stable, and the difference among the weeks is not obvious.

4.5 summary

(1) As can be seen from Table 2, in the halitosis assessment, the number of halitosis cats gradually decreased three weeks before the test, and the number of more severe halitosis cats decreased significantly in the fifth week, and most cats had no halitosis on day 59 with continued feeding of the pet's gel. In the evaluation table 3 of gingivitis, the trend of change is similar to that of halitosis, the treatment effect is better in the early stage of the experiment, and most of gingivitis conditions of cats are effectively controlled after 2 months of the experiment period. The generation of cat halitosis, dental calculus and gingivitis is inseparable from daily dietary habits and physiological conditions, from the whole test process, the conditions of oral diseases of the tested cat are obviously improved, which indicates that the condensation of the pet has cleaning and preventing effects on the oral hygiene of the cat, but in the middle period (the fourth period) of the test, part of cat halitosis and gingivitis recur, the conditions are improved after the test is finished, and the conditions are possibly related to untimely cleaning of test tools, part of cats can unintentionally bring daily ration and hair into basins in daily activities, so that the quality of drinking water in part of basins is disordered, the cleaning frequency of the basins every day is strengthened in the later period, the conditions of cat halitosis and gingivitis are obviously improved and treated, and besides the influence of individual difference, the condensation of the pet has a certain effect on the prevention and treatment of oral problems such as cat halitosis, dental calculus and gingivitis. The product contains effective components such as dipotassium glycyrrhizinate, Kunlun snow chrysanthemum, and a podocarpus macrophyllus extract, wherein the dipotassium glycyrrhizinate is commonly used in the field of medicine including toothpaste and the like, has antibacterial and anti-inflammatory effects, volatile oil rich in the Kunlun snow chrysanthemum has inhibition effects on the biomass of cryptococcus and germ tube germination, the podocarpus macrophyllus extract has certain functions of deodorization and oral cavity cleaning, and the test result shows that the effective components of the product have stronger treatment effects on the oral halitosis of cats.

(2) During the experiment, the DR image of taking on day 0, 13 cats intestinal content is general more, and when taking on day 60, among 13 cats, 7 cats intestinal content is showing and is reducing, explains that use this product to produce and promote the gastrointestinal peristalsis, the effect of recuperating the gastrointestinal function, the herbaceous extract has abundant dietary fiber and has played an important role, add pet condensation simultaneously and promote experimental cat water intake to increase, and then also promoted the moisture increase in the gastrointestinal tract, more do benefit to the discharge of excrement and urine. Can promote digestion of intestines and stomach and effectively improve the gastrointestinal halitosis problem.

(3) Tables 5 and 6 show that the dominant flora is consistent with the results of a number of studies: similar to other mammals, the phyla predominant in the feline gut includes the phyla firmicutes, proteobacteria, bacteroidetes, and actinomycetes. On the level of the species of the compendium, the diversity of each flora is kept good, and the flora imbalance phenomenon does not occur, which indicates that feeding the pet of the cat with water has good protection for maintaining the diversity of the intestinal flora.

(4) The addition of the pet gel had better palatability than ordinary drinking water, based on observations of daily water intake and palatability during the test. From daily water intake, the change range of the whole water intake is stable and tends to increase. From palatability analysis, the pet gel has a pleasant faint scent taste, is suitable in proportion, does not have a contradictory emotion to the pet gel by cats, and can increase the water drinking amount of the cats.

Of course, the present invention may have other embodiments, and based on the embodiments, those skilled in the art can obtain other embodiments without any creative effort, and all of them are within the protection scope of the present invention.

Claims

1. The pet gel with the functions of regulating intestines and stomach and protecting oral health is characterized by comprising the following raw materials in percentage by weight: inulin 0.5-3%, xylo-oligosaccharide 0.1-4%, erythritol 0.5-4%, dipotassium glycyrrhizinate 0.01-2%, herbal extract 0.11-12%, glycerol 1-5%, sodium alginate 0.05-0.5%, vitamin C0.02-0.05%, anhydrous citric acid 0.1-0.5%, sodium benzoate 0.01-0.1%, and the balance of purified water.

2. The pet gel with gastrointestinal conditioning and oral health care functions as claimed in claim 1, wherein the herbal extract is one or more of an extract of podocarpus macrophyllus, petals of Kunlun snow chrysanthemum, an extract of dandelion, petals of honeysuckle, and an extract of houttuynia cordata.

3. The pet gel with gastrointestinal conditioning and oral health care functions as claimed in claim 2, wherein the herbal extract comprises 0.1-7% by weight of podocarpus macrophyllus extract and 0.01-5% by weight of coreopsis tinctoria petals.

4. A method of making a pet gel with gastrointestinal conditioning and oral health benefits in accordance with any one of claims 1 to 3 comprising the steps of: