CN114574334B - Microbial cultivation equipment based on it is even to rotate shake - Google Patents
Microbial cultivation equipment based on it is even to rotate shake Download PDFInfo
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- CN114574334B CN114574334B CN202210361728.1A CN202210361728A CN114574334B CN 114574334 B CN114574334 B CN 114574334B CN 202210361728 A CN202210361728 A CN 202210361728A CN 114574334 B CN114574334 B CN 114574334B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
The invention discloses microbial cultivation equipment based on rotation and uniform shaking, which belongs to the technical field of microbial cultivation and comprises a box body, wherein a temperature control assembly is fixedly arranged in the box body, a box door is hinged to one side of the box body, supporting plates are fixedly connected to two sides in the box body, supporting plates are connected to the tops of the supporting plates in a sliding mode, a plurality of groups of clamping assemblies are movably connected to the tops of the supporting plates, a cavity is formed in each supporting plate, a rotating assembly is movably connected to the interior of the cavity and comprises a rotating disc, and a rotating shaft is fixedly connected to the bottom of the rotating disc. According to the culture dish, the rotating assembly is arranged, the motor drives the connecting rod to rotate through the matching of the first fluted disc and the first transmission chain, the connecting rod drives the first bevel gear to rotate through the sleeve, the first bevel gear drives the rotating disc to rotate through the second bevel gear, and the rotating disc drives the culture dish to rotate, so that the culture dish can be rotated and shaken uniformly, and the growth of microorganisms is facilitated.
Description
Technical Field
The invention belongs to the technical field of microbial culture, and particularly relates to microbial culture equipment based on rotary shaking.
Background
Along with the development and progress of science and technology, the research of human beings on natural science is more and more intensive, and in the meantime, the research on microorganisms is continuously broken through along with the progress of science and technology, microorganisms include bacteria, viruses, fungi, some small-sized protists, microscopic algae and other large groups of organisms, the individuals of which are tiny and closely related to human beings, and through the research on the microorganisms, malignant diseases can be cured, and the life quality of people can be greatly improved.
But current microbial cultivation equipment structure is simple relatively, and current microbial cultivation equipment generally does not possess the rotation and shakes even function, can not provide better culture environment for the microorganism, and current microbial cultivation equipment generally does not possess centre gripping limit function simultaneously, at the in-process of cultivateing the microorganism, if receive external force to influence and make rocking in the culture dish easily and open to the microorganism sample that makes in the culture dish dies.
Disclosure of Invention
Technical problem to be solved
In order to overcome the defects in the prior art, the invention provides microbial cultivation equipment based on rotary shaking, which solves the problems that rotary shaking cannot be carried out and a culture dish cannot be limited and stabilized.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme:
a microbial cultivation device based on rotation and uniform shaking comprises a box body, wherein a temperature control assembly is fixedly arranged in the box body, a box door is hinged to one side of the box body, supporting plates are fixedly connected to two sides of the interior of the box body, supporting plates are slidably connected to the tops of the supporting plates, a plurality of groups of clamping assemblies are movably connected to the tops of the supporting plates, a cavity is formed in each supporting plate, and a rotating assembly is movably connected to the interior of the cavity;
the rotating assembly comprises a rotating disc, a rotating shaft is fixedly connected to the bottom of the rotating disc, one end of the rotating shaft is rotatably connected with the top of the supporting plate through a bearing and extends into the cavity, a second bevel gear is fixedly connected to one end, away from the rotating disc, of the rotating shaft, the bottom of the second bevel gear is meshed with a first bevel gear, a sleeve is clamped inside the first bevel gear, the sleeve is rotatably connected with two sides of the wall of the cavity through the bearing, and one end of the sleeve penetrates through the cavity and extends to the outer side of the supporting plate;
the connecting rod is connected in the sleeve in a sliding mode, the cross sections of the connecting rod and the inner wall of the sleeve are regular hexagons, one end of the connecting rod is fixedly connected with a first fluted disc through a rotating shaft, the first fluted discs are in transmission connection through a first transmission chain, one end, far away from the connecting rod, of each first fluted disc is fixedly connected with a motor, and the motor is fixedly installed on one side of the box body through an installation plate;
the two sides of the bottom of the rotating disc are fixedly connected with first telescopic rods, the bottom of each first telescopic rod is fixedly connected with a connecting seat, a first spring is sleeved on the outer surface of each first telescopic rod, two ends of each first spring are fixedly connected with the bottom of the rotating disc and the top of the connecting seat respectively, the bottom of each connecting seat is rotatably connected with a guide rail wheel, the bottom of each guide rail wheel is connected with a slide rail in a sliding manner, and the bottom of each slide rail is fixedly connected with the top of the supporting plate;
the rotating disc bottom fixedly connected with counter weight iron plate, the magnet piece has been placed to counter weight iron plate bottom, magnet piece bottom and layer board top fixed connection, counter weight iron plate, magnet piece and slide rail cross sectional shape are the annular.
As a further scheme of the invention:
the clamping assembly comprises two connecting plates, sliding blocks are fixedly connected to the bottoms of the connecting plates, the sliding blocks are in sliding connection with sliding grooves formed in the tops of the supporting plates and extend into the cavity, the cavity is communicated with the sliding grooves, a sliding rod is connected to the inside of one sliding block in a sliding mode, two ends of each sliding rod are fixedly connected to two sides of the inner wall of the cavity, moving screws are connected to the insides of the other sliding blocks in a threaded mode, the moving screws are symmetrically distributed along the axial center position, the thread directions of two sides of each moving screw are opposite, two adjacent moving screws are fixedly connected through connecting shafts, two ends of each moving screw are rotatably connected with two sides of the inner wall of the cavity through rotating shafts and bearings and extend to the outer side of the supporting plate, transmission gears are fixedly connected to two ends of each moving screw, racks are meshed with the tops of the transmission gears, and are fixedly connected with the inner walls of the box body;
the outer wall of the connecting shaft is clamped with second toothed discs, and the second toothed discs are in transmission connection through second transmission chains;
connecting plate one side fixedly connected with connecting block, a plurality of second telescopic links of connecting block one side fixedly connected with, connecting block one side fixedly connected with grip block is kept away from to the second telescopic link, second telescopic link surface cover is equipped with the second spring, second spring both sides respectively with connecting block one side and grip block one side fixed connection, the grip block inner wall inlays and is equipped with a plurality of balls.
As a further scheme of the invention:
the temperature control assembly comprises a heating water tank, the heating water tank is fixedly connected with the bottom of the interior of the box body, two circulating pumps are fixedly connected to the top of the heating water tank, circulating pipes are fixedly connected between the two circulating pumps, the circulating pipes are fixedly mounted on the inner wall of the backboard of the box body, connecting pipes are fixedly connected to the two ends of the heating water tank, the connecting pipes are embedded in the two sides of the inner wall of the box body, a plurality of atomizing spray heads are fixedly connected to one side of each connecting pipe, and the atomizing spray heads penetrate through the inner wall of the box body and extend into the box body.
As a further scheme of the invention:
one side of the box body is fixedly provided with a control panel, the periphery of the bottom of the box body is fixedly connected with moving wheels, and one side of the box door is embedded with an observation window.
A use method of microorganism culture equipment based on rotary shaking comprises the following steps:
s1, manufacturing a microbial culture dish;
s2, placing a culture dish, pulling out a supporting plate after an operator opens a box door, wherein in the process, the supporting plate drives a sleeve to slide on the outer surface of a connecting rod, the supporting plate drives a transmission gear to move, the transmission gear rotates under the drive of a rack, the transmission gear drives a movable lead screw to rotate, meanwhile, a connecting shaft drives the rest movable lead screws to rotate through a second fluted disc and a second transmission chain, the movable lead screws drive two sliding blocks to slide on the outer surfaces of sliding rods, the two sliding blocks drive connecting plates to move, the two connecting plates are far away from each other, the two connecting blocks drive clamping blocks to be far away from each other, after the supporting plate slides out of a box body, the operator places the culture dish on the top of a rotating disk, the culture dish drives the rotating disk to move downwards under the action of the self weight, meanwhile, a counter weight iron block moves downwards under the drive of a magnet block, a second telescopic rod contracts and drives a second spring to contract, the rotating disk drives a rotating shaft to move downwards, and the rotating shaft drives a second bevel gear to be meshed with a first bevel gear;
s3, pushing the culture dish into the box body, pushing the supporting plate into the box body again by a worker, reversely rotating the transmission gear and driving the movable lead screws to reversely rotate, driving the rest movable lead screws to reversely rotate by the connecting shaft through the second fluted disc and the second transmission chain, driving the clamping blocks to mutually approach and clamp the culture dish by the movable lead screws, and closing the box door by the worker after the supporting plate slides into the box body;
s4, rotating the culture dish, turning on a motor by a worker, driving a first fluted disc to rotate by the motor, driving the other first fluted discs to rotate by the first fluted disc through a first transmission chain, driving a connecting rod to rotate by the first fluted disc through a rotating shaft, driving a sleeve to rotate by the connecting rod, driving a first bevel gear to rotate by the sleeve, driving a second bevel gear to drive a rotating shaft to rotate by the first bevel gear, driving the rotating disc to rotate by the rotating shaft, driving the culture dish to rotate by the rotating disc, and matching the culture dish with balls on the inner wall of the clamping block to rotate;
s5, controlling temperature through operation, culturing, adjusting the water temperature in the heating water tank through an operation control panel by a worker, circularly flowing hot water in the circulating pipe through the circulating pump, keeping the temperature in the box body, and intermittently spraying water mist into the box body through the connecting pipe and the atomizing nozzle by the heating water tank.
(III) advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
1. this a microbial cultivation equipment based on it is even to rotate to shake, through setting up rotating assembly, the culture dish drives the rolling disc downstream under the effect of gravity, the magnet piece attracts the decurrent gravity of increase rolling disc to the counter weight iron plate, make the rolling disc drive second bevel gear through the axis of rotation and mesh with first bevel gear, can realize that the rolling disc of placing the culture dish can just rotate, vacant rolling disc can not rotate, can reduce motor unnecessary power consumption, and simultaneously, the motor drives the connecting rod through first fluted disc and first drive chain's cooperation and rotates, the connecting rod passes through the sleeve and rotates to driving first bevel gear, first bevel gear drives the rolling disc through second bevel gear and rotates, the rolling disc drives the culture dish and rotates, can realize shaking the rotation of culture dish evenly, be favorable to the growth of microorganism.
2. This a microbial cultivation equipment based on it is even to rotate shake, through setting up the centre gripping subassembly, when taking out the layer board, drive gear rotates and drives the removal lead screw rotation under the drive of rack, it drives the connecting plate through two sliders to move to remove the removal lead screw, two connecting plates are kept away from each other, two connecting blocks drive the centre gripping and keep away from each other, remove the spacing firm to the culture dish, when the layer board slides in the box, drive gear antiport drives the removal lead screw antiport, it is close to each other and carries out the centre gripping spacing to the culture dish to remove the lead screw, can realize spacing and the separation of the automatic centre gripping of culture dish on the one hand, make things convenient for the access of staff to the culture dish, reduce the operation flow, and improve work efficiency, and simultaneously, can realize spacing to the culture dish, cooperation ball can reduce the frictional force when the culture dish rotates, make things convenient for the culture dish to rotate, avoid the culture dish to break away from the carousel in rotatory in-process.
3. According to the invention, through the arrangement of the temperature control assembly, a worker can adjust the water temperature in the heating water tank by operating the control panel, hot water circularly flows in the circulating pipe through the circulating pump, the temperature in the box body can be adjusted and kept constant, meanwhile, the heating water tank intermittently sprays water mist into the box body through the connecting pipe and the atomizing nozzle, the humidity in the box body can be controlled, an environment suitable for growth can be provided for microorganisms by controlling the temperature and the moisture in the box body, and the survival rate of the microorganisms is improved.
Drawings
FIG. 1 is a schematic perspective view of a microorganism culture apparatus based on rotary shaking according to the present invention;
FIG. 2 is a schematic structural diagram of a cross section in front view of a microorganism culture apparatus based on rotational shaking according to the present invention;
FIG. 3 is a schematic top view of a cross-sectional structure of a rotating shaking-based supporting plate of a microorganism culture apparatus according to the present invention;
FIG. 4 is a schematic diagram of a side view cross-sectional structure of a rotating shaking-based microorganism culture apparatus pallet according to the present invention;
FIG. 5 is an enlarged schematic structural diagram of part A of a microorganism culture apparatus based on rotary shaking according to the present invention;
FIG. 6 is an enlarged schematic structural diagram of part B of a microorganism culture apparatus based on rotary shaking according to the present invention;
FIG. 7 is a schematic diagram of a temperature control assembly of a microorganism culture apparatus based on rotation shaking according to the present invention.
Illustration of the drawings: 1. a box body; 2. a control panel; 3. an observation window; 4. a box door; 5. a moving wheel; 6. a rotating assembly; 601. a first fluted disc; 602. a connecting rod; 603. a first bevel gear; 604. a second bevel gear; 605. a sleeve; 606. a rotating shaft; 607. rotating the disc; 608. a first telescopic rod; 609. a first spring; 610. a connecting seat; 611. a guide rail wheel; 612. a slide rail; 613. a magnet block; 614. a counterweight iron block; 615. a first drive chain; 616. a motor; 7. a clamping assembly; 701. a slide bar; 702. a connecting plate; 703. connecting blocks; 704. a transmission gear; 705. moving the lead screw; 706. a connecting shaft; 707. a clamping block; 708. a ball bearing; 709. a second telescopic rod; 710. a second spring; 711. a second fluted disc; 712. a second drive chain; 713. a slider; 714. a chute; 715. a rack; 8. a temperature control assembly; 801. heating the water tank; 802. a circulation pump; 803. an atomizing spray head; 804. a connecting pipe; 805. a circulation pipe; 9. a support plate; 10. a support plate; 11. a cavity.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to 7, the present invention provides a technical solution: a microbial cultivation device based on rotary shaking comprises a box body 1, wherein a temperature control assembly 8 is fixedly arranged in the box body 1, a box door 4 is hinged to one side of the box body 1, supporting plates 10 are fixedly connected to two sides of the interior of the box body 1, supporting plates 9 are slidably connected to the tops of the supporting plates 10, a plurality of groups of clamping assemblies 7 are movably connected to the tops of the supporting plates 9, a cavity 11 is formed in each supporting plate 9, a rotating assembly 6 is movably connected to the interior of each cavity 11, a control panel 2 is fixedly arranged on one side of the box body 1, moving wheels 5 are fixedly connected to the periphery of the bottom of the box body 1, and an observation window 3 is embedded in one side of the box door 4;
Through setting up temperature control assembly 8, hot water passes through circulating pump 802 at circulating pipe 805 inner loop flow, heating water tank 801 intermittent type nature is through connecting pipe 804 and atomizer 803 spray water in to box 1, can control the humidity in the box 1, can adjust and make the temperature in the box 1 keep invariable, temperature and moisture humidity in through the control box 1, can provide an environment that is fit for growing for the microorganism, improve the microorganism survival rate, improve the cultivation quality of cultivation equipment, he is through setting up observation window 3, make things convenient for the staff to observe the culture dish in the box 1, make things convenient for the worker master personnel to adjust the cultivation environment in the cultivation equipment according to actual conditions.
Specifically, as shown in fig. 2-5, the clamping assembly 7 includes two connecting plates 702, the bottom of each connecting plate 702 is fixedly connected with a sliding block 713, each sliding block 713 is slidably connected with a sliding slot 714 formed at the top of the supporting plate 9 and extends into the cavity 11, the cavity 11 is communicated with the sliding slot 714, a sliding rod 701 is slidably connected with the inside of one sliding block 713, two ends of each sliding rod 701 are fixedly connected with two sides of the inner wall of the cavity 11, the inner threads of the other sliding block 713 are connected with movable screws 705, the movable screws 705 are symmetrically distributed along the axial center position, the thread directions of two sides of each movable screw 705 are opposite, two adjacent movable screws 705 are fixedly connected through a connecting shaft 706, two ends of each movable screw 705 are rotatably connected with two sides of the inner wall of the cavity 11 through a rotating shaft and a bearing and extend to the outer side of the supporting plate 9, the two ends of the movable screw 705 are fixedly connected with transmission gears 704, the tops of the transmission gears 704 are meshed with racks 715, the racks 715 are fixedly connected with the inner wall of the box body 1, second gear discs 711 are clamped on the outer wall of the connecting shaft 706, the second gear discs 711 are in transmission connection with one another through second transmission chains 712, connecting blocks 703 are fixedly connected to one side of the connecting plate 702, a plurality of second telescopic rods 709 are fixedly connected to one side of the connecting block 703, the second telescopic rods 709 are far away from clamping blocks 707 fixedly connected to one side of the connecting block 703, second springs 710 are sleeved on the outer surfaces of the second telescopic rods 709, two sides of the second springs 710 are fixedly connected with one side of the connecting block 703 and one side of the clamping blocks 707 respectively, and a plurality of balls 708 are embedded in the inner wall of the clamping blocks 707
Through setting up centre gripping subassembly 7, when taking out layer board 9, drive gear 704 rotates and drives two grip blocks 707 through a series of transmission under rack 715's drive and keeps away from each other, relieve the spacing firm to the culture dish, when layer board 9 slips into in the box 1, drive gear 704 antiport drives two grip blocks 707 and is close to each other and carries out the centre gripping spacing to the culture dish, can realize spacing and the separation of automatic centre gripping to the culture dish on the one hand, make things convenient for the access of staff to the culture dish, reduce the operation flow, improve work efficiency, and simultaneously, can realize spacing to the culture dish, cooperation ball 708 can reduce the frictional force when the culture dish rotates, make things convenient for the culture dish to rotate, avoid the culture dish to break away from the carousel at rotatory in-process.
Specifically, as shown in fig. 2, 4 and 6, the rotating assembly 6 includes a rotating disc 607, a rotating shaft 606 is fixedly connected to the bottom of the rotating disc 607, one end of the rotating shaft 606 is rotatably connected to the top of the supporting plate 9 through a bearing and extends into the cavity 11, a second bevel gear 604 is fixedly connected to one end of the rotating shaft 606 away from the rotating disc 607, a first bevel gear 603 is engaged with the bottom of the second bevel gear 604, a sleeve 605 is clamped inside the first bevel gear 603, the sleeve 605 is rotatably connected to two sides of the wall of the cavity 11 through a bearing, one end of the sleeve 605 penetrates through the cavity 11 and extends to the outside of the supporting plate 9, a connecting rod 602 is slidably connected in the sleeve 605, the cross-sectional shapes of the inner walls of the connecting rod 602 and the sleeve 605 are both regular hexagons, one end of the connecting rod 602 is fixedly connected to a first fluted disc 601 through a rotating shaft, and the first fluted discs 601 are drivingly connected through a first transmission chain 615, and wherein one end fixedly connected with motor 616 that connecting rod 602 was kept away from to first fluted disc 601, motor 616 passes through mounting panel and box 1 one side fixed mounting, the equal fixedly connected with first telescopic link 608 in rotating disc 607 bottom both sides, first telescopic link 608 bottom fixedly connected with connecting seat 610, first telescopic link 608 surface cover is equipped with first spring 609, first spring 609 both ends respectively with rotating disc 607 bottom and connecting seat 610 top fixed connection, connecting seat 610 bottom is rotated and is connected with guide rail wheel 611, guide rail wheel 611 bottom sliding connection has slide rail 612, slide rail 612 bottom and layer board 9 top fixed connection, rotating disc 607 bottom fixedly connected with counter weight iron 614, magnet piece 613 has been placed to counter weight iron 614 bottom, magnet piece 613 bottom and layer board 9 top fixed connection, counter weight iron 614, magnet piece 613 and slide rail 612 cross section shape are the annular.
Through setting up rotating assembly 6, the culture dish drives rolling disc 607 downstream under the effect of gravity, magnet piece 613 attracts the decurrent gravity of increase rolling disc 607 to counter weight iron plate 614, make rolling disc 607 drive second bevel gear through axis of rotation 606 and mesh with first bevel gear 603 mutually, can realize that the rolling disc 607 of placing the culture dish just can rotate, vacant rolling disc 607 can not rotate, can reduce the unnecessary power consumption of motor 616, and simultaneously, motor 616 drives connecting rod 602 through the cooperation of first fluted disc 601 and first drive chain 615 and rotates, connecting rod 602 passes through sleeve 605 and rotates to driving first bevel gear 603, first bevel gear 603 drives rolling disc 607 through second bevel gear 604 and rotates, rolling disc 607 drives the culture dish and rotates, can realize shaking up the rotation of culture dish, be favorable to the growth of microorganism.
The working principle of the invention is as follows:
s1, manufacturing a microbial culture dish;
s2, placing a culture dish, pulling out a supporting plate 9 after a worker opens a box door 4, in the process, the supporting plate 9 drives a sleeve 605 to slide on the outer surface of a connecting rod 602, the supporting plate 9 drives a transmission gear 704 to move, the transmission gear 704 is driven by a rack 715 to rotate, the transmission gear 704 drives a movable lead screw 705 to rotate, meanwhile, a connecting shaft 706 drives the rest movable lead screws 705 to rotate through a second fluted disc 711 and a second transmission chain 712, the movable lead screw 705 drives two sliding blocks 713 to slide on the outer surface of a sliding rod 701, the two sliding blocks 713 drive connecting plates 702 to move, the two connecting plates 702 are far away from each other, the two connecting blocks 703 drive clamping blocks 707 to be far away from each other, after the supporting plate 9 slides out of a box body 1, the worker places the culture dish on the top of the rotating disc 607, the culture dish drives the rotating disc 607 to move downwards under the action of the self weight of the culture dish, meanwhile, a counterweight iron block 614 moves downwards under the drive of a magnet block 613, a second telescopic rod 709 contracts and drives a second spring 710 to contract, the rotating disc 607 to move downwards, the rotating disc 607 drives a rotating shaft 606 to drive a second bevel gear to be meshed with a first bevel gear 603;
s3, pushing the culture dish into the box body 1, pushing the supporting plate 9 into the box body 1 again by a worker, reversely rotating the transmission gear 704 and driving the movable lead screw 705 to reversely rotate, driving the rest movable lead screws 705 to reversely rotate by the connecting shaft 706 through the second fluted disc 711 and the second transmission chain 712, driving the clamping blocks 707 by the movable lead screws 705 to mutually approach and clamp the culture dish, and closing the box door 4 by the worker after the supporting plate 9 slides into the box body 1;
s4, the culture dish rotates, a worker turns on a motor 616, the motor 616 drives a first fluted disc 601 to rotate, the first fluted disc 601 drives the rest of first fluted discs 601 to rotate through a first transmission chain 615, the first fluted disc 601 drives a connecting rod 602 to rotate through a rotating shaft, the connecting rod 602 drives a sleeve 605 to rotate, the sleeve 605 drives a first bevel gear 603 to rotate, the first bevel gear 603 drives a second bevel gear 604 to drive a rotating shaft 606 to rotate, the rotating shaft 606 drives a rotating disc 607 to rotate, the rotating disc 607 drives the culture dish to rotate, and balls 708 on the inner wall of a clamping block 707 are matched with the culture dish to rotate;
s5, controlling temperature and culturing, wherein a worker adjusts the water temperature in the heating water tank 801 by operating the control panel 2, hot water circularly flows in the circulating pipe 805 through the circulating pump 802 to keep the temperature in the box body 1, and meanwhile, the heating water tank 801 intermittently sprays water mist into the box body 1 through the connecting pipe 804 and the atomizing nozzle 803.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
Although the preferred embodiments of the present patent have been described in detail, the present patent is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present patent within the knowledge of those skilled in the art.
Claims (5)
1. The microbial cultivation equipment based on rotary shaking comprises a box body (1) and is characterized in that a temperature control assembly (8) is fixedly arranged inside the box body (1), a box door (4) is hinged to one side of the box body (1), supporting plates (10) are fixedly connected to two sides inside the box body (1), a supporting plate (9) is connected to the top of each supporting plate (10) in a sliding mode, a plurality of groups of clamping assemblies (7) are movably connected to the top of each supporting plate (9), a cavity (11) is formed inside each supporting plate (9), and a rotating assembly (6) is movably connected to the inside of each cavity (11);
the rotating assembly (6) comprises a rotating disc (607), a rotating shaft (606) is fixedly connected to the bottom of the rotating disc (607), one end of the rotating shaft (606) is rotatably connected with the top of the supporting plate (9) through a bearing and extends into the cavity (11), a second bevel gear (604) is fixedly connected to one end, away from the rotating disc (607), of the rotating shaft (606), a first bevel gear (603) is meshed with the bottom of the second bevel gear (604), a sleeve (605) is clamped inside the first bevel gear (603) in a clamping manner, the sleeve (605) is rotatably connected with two sides of the wall of the cavity (11) through bearings, and one end of the sleeve (605) penetrates through the cavity (11) and extends to the outer side of the supporting plate (9);
a connecting rod (602) is connected in the sleeve (605) in a sliding manner, the cross section shapes of the inner walls of the connecting rod (602) and the sleeve (605) are regular hexagons, one end of the connecting rod (602) is fixedly connected with a first fluted disc (601) through a rotating shaft, the first fluted discs (601) are in transmission connection through a first transmission chain (615), one end, far away from the connecting rod (602), of each first fluted disc (601) is fixedly connected with a motor (616), and the motor (616) is fixedly installed on one side of the box body (1) through a mounting plate;
both sides of the bottom of the rotating disc (607) are fixedly connected with first telescopic rods (608), the bottom of each first telescopic rod (608) is fixedly connected with a connecting seat (610), a first spring (609) is sleeved on the outer surface of each first telescopic rod (608), two ends of each first spring (609) are fixedly connected with the bottom of the rotating disc (607) and the top of the connecting seat (610), the bottom of the connecting seat (610) is rotatably connected with a guide rail wheel (611), the bottom of the guide rail wheel (611) is slidably connected with a slide rail (612), and the bottom of the slide rail (612) is fixedly connected with the top of the supporting plate (9);
rotating disc (607) bottom fixedly connected with counter weight iron plate (614), magnet piece (613) have been placed to counter weight iron plate (614) bottom, magnet piece (613) bottom and layer board (9) top fixed connection, counter weight iron plate (614), magnet piece (613) and slide rail (612) cross sectional shape are the annular.
2. The microorganism culture equipment based on rotation shaking up of claim 1, wherein the clamping assembly (7) comprises two connecting plates (702), sliding blocks (713) are fixedly connected to the bottoms of the connecting plates (702), the sliding blocks (713) are slidably connected with sliding grooves (714) formed in the tops of supporting plates (9) and extend into a cavity (11), the cavity (11) is communicated with the sliding grooves (714), a sliding rod (701) is slidably connected inside one sliding block (713), two ends of the sliding rod (701) are fixedly connected with two sides of the inner wall of the cavity (11), a movable lead screw (705) is connected with the inner thread of the other sliding block (713) in a threaded mode, the movable lead screws (705) are symmetrically distributed along the axial center position, the thread directions of two sides of the movable lead screws (715) are opposite, two adjacent movable lead screws (705) are fixedly connected through connecting shafts (706), two ends of each movable lead screw (705) are rotatably connected with two sides of the inner wall of the cavity (11) through rotating shafts and extend to the outer side of the supporting plate (9), two ends of each movable lead screw (704) are fixedly connected with a transmission gear (705), a rack (704) is meshed with a rack (704), and the top of the box body (1);
the outer wall of the connecting shaft (706) is clamped with a second toothed disc (711), and the second toothed discs (711) are in transmission connection through a second transmission chain (712);
connecting plate (702) one side fixedly connected with connecting block (703), a plurality of second telescopic links (709) of connecting block (703) one side fixedly connected with, connecting block (703) one side fixedly connected with grip block (707) are kept away from to second telescopic link (709), the surface cover of second telescopic link (709) is equipped with second spring (710), second spring (710) both sides respectively with connecting block (703) one side and grip block (707) one side fixed connection, grip block (707) inner wall inlays and is equipped with a plurality of balls (708).
3. The microbial cultivation equipment based on even rotation of shaking as claimed in claim 1, wherein the temperature control assembly (8) comprises a heating water tank (801), the heating water tank (801) is fixedly connected with the bottom inside the box body (1), two circulating pumps (802) are fixedly connected with the top of the heating water tank (801), a circulating pipe (805) is fixedly connected between the two circulating pumps (802), the circulating pipe (805) is fixedly installed on the inner wall of the back plate of the box body (1), connecting pipes (804) are fixedly connected with both ends of the heating water tank (801), the connecting pipes (804) are embedded on both sides of the inner wall of the box body (1), a plurality of atomization nozzles (803) are fixedly connected on one side of the connecting pipes (804), and the atomization nozzles (803) extend to the inside of the box body (1) through the inner wall of the box body (1).
4. The microbial cultivation equipment based on even rotation and shaking as claimed in claim 1, wherein a control panel (2) is fixedly arranged on one side of the box body (1), moving wheels (5) are fixedly connected to the periphery of the bottom of the box body (1), and an observation window (3) is embedded in one side of the box door (4).
5. The use method of the microorganism culture equipment based on the rotary shaking up according to any one of claims 1 to 4, characterized by comprising the following steps:
s1, manufacturing a microbial culture dish;
s2, placing a culture dish, pulling out the supporting plate (9) after a worker opens the box door (4), in the process, the supporting plate (9) drives the sleeve (605) to slide on the outer surface of the connecting rod (602), the supporting plate (9) drives the transmission gear (704) to move, the transmission gear (704) is driven by the rack (715) to rotate, the transmission gear (704) drives the movable screw rod (705) to rotate, meanwhile, the connecting shaft (706) drives the rest of the movable lead screws (705) to rotate through the second fluted disc (711) and the second transmission chain (712), the movable lead screws (705) drive the two sliding blocks (713) to slide on the outer surfaces of the sliding rods (701), the two sliding blocks (713) drive the connecting plates (702) to move, the two connecting plates (702) are far away from each other, the two connecting blocks (703) drive the clamping blocks (707) to be far away from each other, and after the supporting plate (9) slides out of the box body (1), the operator places the culture dish on the top of the rotating disc (607), the culture dish drives the rotating disc (607) to move downwards under the action of the self weight, meanwhile, the counterweight iron block (614) moves downwards under the drive of the magnet block (613), the second telescopic rod (709) contracts and drives the second spring (710) to contract, the rotating disc (607) drives the rotating shaft (606) to move downwards, and the rotating shaft (606) drives the second bevel gear to be meshed with the first bevel gear (603);
s3, pushing the culture dish into the box body (1), pushing the supporting plate (9) into the box body (1) again by a worker, reversely rotating the transmission gear (704) and driving the movable screw rod (705) to reversely rotate, driving the rest movable screw rods (705) to reversely rotate by the connecting shaft (706) through the second fluted disc (711) and the second transmission chain (712), driving the clamping blocks (707) to mutually approach and clamp the culture dish by the movable screw rod (705), and closing the box door (4) by the worker after the supporting plate (9) slides into the box body (1);
s4, the culture dish rotates, a worker turns on a motor (616), the motor (616) drives a first fluted disc (601) to rotate, the first fluted disc (601) drives other first fluted discs (601) to rotate through a first transmission chain (615), the first fluted disc (601) drives a connecting rod (602) to rotate through a rotating shaft, the connecting rod (602) drives a sleeve (605) to rotate, the sleeve (605) drives a first bevel gear (603) to rotate, the first bevel gear (603) drives a second bevel gear (604) to drive a rotating shaft (606) to rotate, the rotating shaft (606) drives a rotating disc (607) to rotate, the rotating disc (607) drives the culture dish to rotate, and balls (708) on the inner wall of a clamping block (707) are matched with the culture dish to rotate;
s5, controlling temperature and culturing, wherein a worker adjusts the water temperature in the heating water tank (801) by operating the control panel (2), hot water circularly flows in the circulating pipe (805) through the circulating pump (802) to keep the temperature in the box body (1), and meanwhile, the heating water tank (801) intermittently sprays water mist in the box body (1) through the connecting pipe (804) and the atomizing nozzle (803).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210361728.1A CN114574334B (en) | 2022-04-07 | 2022-04-07 | Microbial cultivation equipment based on it is even to rotate shake |
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| CN202210361728.1A CN114574334B (en) | 2022-04-07 | 2022-04-07 | Microbial cultivation equipment based on it is even to rotate shake |
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| CN114574334B true CN114574334B (en) | 2022-12-27 |
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| CN115574570B (en) * | 2022-10-09 | 2024-05-03 | 安徽茗驰电子科技有限公司 | Circuit board drying device |
| CN118222372A (en) * | 2024-03-28 | 2024-06-21 | 中安方硕(江苏)生物科技有限公司 | Microorganism culture device |
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| CN103597065B (en) * | 2011-06-14 | 2016-08-17 | 日商乐华股份有限公司 | There is the thermostat of rotating sample |
| CN208545434U (en) * | 2018-05-15 | 2019-02-26 | 李国英 | A kind of clinical laboratory's anti-pollution type germ culturing device |
| CN209368240U (en) * | 2018-12-10 | 2019-09-10 | 枫木年轮生物科技（广州）有限公司 | Biological medicine culture dish adjustable support |
| CN212833823U (en) * | 2020-06-08 | 2021-03-30 | 浙江海福生物科技有限责任公司 | Stem cell amplification device |
| CN113832009A (en) * | 2021-09-24 | 2021-12-24 | 曾峰 | A rotatable formula cell culture dish for cell culture |
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