CN114570057A - Method for extracting inhibitor from multi-element substance - Google Patents
Method for extracting inhibitor from multi-element substance Download PDFInfo
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- CN114570057A CN114570057A CN202210214916.1A CN202210214916A CN114570057A CN 114570057 A CN114570057 A CN 114570057A CN 202210214916 A CN202210214916 A CN 202210214916A CN 114570057 A CN114570057 A CN 114570057A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D36/00—Filter circuits or combinations of filters with other separating devices
- B01D36/04—Combinations of filters with settling tanks
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H5/00—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium
- C07H5/04—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium to nitrogen
- C07H5/06—Aminosugars
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
Abstract
The invention discloses a method for extracting an inhibitor from a multi-element substance, which comprises the following working steps: (1) preparing herbaceous plants to be extracted in advance; (2) extracting the cleaned herbs to obtain extracts and mixtures; (3) precipitating the extract and the mixture to obtain a clarified liquid; (4) cleaning the clarified liquid to remove impurities to obtain filtrate; (5) concentrating the filtrate under high pressure to obtain concentrated solution; (6) extracting the concentrated solution to obtain an extract; (7) concentrating and drying the extract to obtain the inhibitor. According to the invention, the inhibitor is extracted from herbaceous plants such as mulberries, liquorice, white paeony roots, purple perilla, kudzuvine roots, Chinese yams and dandelions, active probiotics, vitamins A, B1, B2, B6, B12, folic acid, pantothenic acid, nicotinic acid, C, D, E and nutrients such as calcium, magnesium, zinc, selenium, iron, copper and manganese, so that the extraction purity of the inhibitor is good.
Description
Technical Field
The invention relates to the technical field of element extraction, in particular to a method for extracting an inhibitor from a multi-element substance.
Background
The inhibitor is 5-amino-5-deoxy-D-glucose produced by some streptomyces, is an antibiotic, and can be used as an inhibitor of intestinal alpha glucosidase, pancreatic alpha amylase, alpha glucosidase in N-sugar chain processing, and the like, after forming a pyranoid ring, similarly to glucopyranose.
The method for extracting the inhibitor from the multielement substance in the prior art has the defects that:
1. patent document CN107311912B discloses a method for extracting 1-deoxynojirimycin from silkworm excrement, which comprises the following steps: (1) carrying out continuous countercurrent extraction on clean silkworm excrement to obtain a silkworm excrement extracting solution and a pasty mixture; (2) performing flocculation precipitation on the extracting solution to obtain a clarified solution; (3) carrying out ultrafiltration and impurity removal on the clarified solution to obtain an ultrafiltration permeate; (4) carrying out nanofiltration concentration on the ultrafiltration permeating liquid to obtain nanofiltration concentrated liquid; (5) extracting and separating the nanofiltration concentrated solution to obtain an extract liquid; (6) concentrating and drying the extract liquor to obtain a finished product containing 1-deoxynojirimycin, wherein the mass fraction of the 1-deoxynojirimycin in the finished product is 20-30%. The method is simple and convenient to operate, low in cost, energy-saving and environment-friendly, and can be used for industrial production. When the device is used, the purity of the inhibitor needs to be improved when the existing extraction method is used, but most of the extraction methods have poor effect, so that the extraction method is inconvenient to use.
2. In the prior art, when the method for extracting the inhibitor from the multi-element substance is used, workers need to precipitate the extract, but the existing extraction method has a relatively common effect during precipitation and relatively complex use steps, so that the precipitation effect is relatively difficult.
3. In the prior art, when the method for extracting the inhibitor from the multi-element substance is used, workers need to reduce impurities in the inhibitor, but the existing extraction method causes more impurities in the inhibitor, so that the inhibitor is inconvenient to use, and the extraction method is limited.
Disclosure of Invention
The present invention has been made to solve the above-mentioned problems occurring in the prior art, and an object of the present invention is to provide a method for extracting an inhibitor from a multi-element substance.
In order to achieve the above object, the present invention provides a method for extracting an inhibitor from a multi-element substance, comprising the following steps:
(1) preparing herbaceous plants to be extracted in advance;
(2) extracting the cleaned herbs to obtain extracts and mixtures;
(3) precipitating the extract and the mixture to obtain a clarified liquid;
(4) cleaning the clarified liquid to remove impurities to obtain filtrate;
(5) concentrating the filtrate under high pressure to obtain concentrated solution;
(6) extracting the concentrated solution to obtain an extract;
(7) concentrating and drying the extract to obtain the inhibitor.
Preferably, in the step (1), the worker prepares and cleans herbs such as mulberry, licorice, white peony root, perilla, kudzu vine root, yam and dandelion, prepares active probiotics, vitamins A, B1, B2, B6, B12, folic acid, pantothenic acid, nicotinic acid, C, D, E and calcium, magnesium, zinc, selenium, iron, copper and manganese in advance, and prepares the herbs in advance.
Preferably, in the step (2), the worker uniformly adds water into the herbs such as mulberry, liquorice, white peony root, perilla, kudzu vine root, Chinese yam and dandelion, extracts the herbs for one hour by extrusion to obtain an extract, and mixes the elements at a high temperature to obtain a mixture.
Preferably, in the step (3), the worker mixes the extract of the herb and the mixture of the elements together, and precipitates the mixture of the various extracts and the mixture of the elements for three hours, so that it is convenient to obtain a clear liquid and a solid mixture.
Preferably, in the step (4), the clear liquid is filtered and concentrated by a worker, so that salt, ash, water and small molecular impurities are removed from the clear liquid, and a filtrate is obtained.
Preferably, in the step (5), the operator performs a scraper film decompression on the filtrate, and then compresses the filtrate at high temperature and high pressure to obtain a concentrated solution.
Preferably, in the step (6), the worker adds an alkali solution to the concentrated solution to adjust the pH value to nine to eleven so that the concentrated solution is in a free state, adds the same lipophilic solvent into the concentrated solution, stirs the mixture for one hour at normal temperature, and stands the mixture for layering to obtain the extract.
Preferably, in the step (7), the worker concentrates the extract liquid into paste, the paste extract liquid is spray-dried to obtain the inhibitor, and the paste extract liquid in pure water is five to seven times.
Compared with the prior art, the invention has the following beneficial effects:
1. the inhibitor is extracted from herbaceous plants such as mulberry, liquorice, white paeony root, purple perilla, kudzuvine root, Chinese yam and dandelion, active probiotics, vitamin A, B1, B2, B6, B12, folic acid, pantothenic acid, nicotinic acid, C, D, E and nutrients such as calcium, magnesium, zinc, selenium, iron, copper and manganese, so that the extraction purity of the inhibitor is better.
2. According to the invention, the extract of the herbaceous plant and the element mixture are subjected to standing precipitation for three hours to obtain a pure clear liquid and solid mixture, so that the simple and easy precipitation is facilitated for workers and is simple.
3. According to the invention, the clarified liquid is filtered and concentrated, so that salt, ash, water and small molecular impurities are removed from the clarified liquid, and the impurities in the extracting solution are conveniently removed, thereby facilitating the extraction of the inhibitor by workers.
Drawings
FIG. 1 is a flow chart of the structure of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1, according to an embodiment of the present invention, a method for extracting an inhibitor from a multi-element substance includes the following steps: (1) preparing herbaceous plants to be extracted in advance; (2) extracting the cleaned herbs to obtain extracts and mixtures; (3) precipitating the extract and the mixture to obtain a clarified liquid; (4) cleaning the clarified liquid to remove impurities to obtain filtrate; (5) concentrating the filtrate under high pressure to obtain concentrated solution; (6) extracting the concentrated solution to obtain an extract liquid; (7) concentrating and drying the extract to obtain an inhibitor; in the step (1), workers prepare and clean herbaceous plants such as mulberries, liquorice, white paeony roots, purple perilla, radix puerariae, Chinese yams and dandelions and prepare active probiotics, vitamins A, B1, B2, B6, B12, folic acid, pantothenic acid, nicotinic acid, C, D, E and calcium, magnesium, zinc, selenium, iron, copper and manganese in advance, the herbaceous plants such as mulberries, liquorice, white paeony roots, purple perilla, radix puerariae, Chinese yams and dandelions are uniformly added with water and are extruded for one hour to obtain extract liquor, various elements are mixed at high temperature to obtain a mixture, in the step (3), the extracts of the herbaceous plants and the element mixture are mixed together by the workers, and the mixture of the various extracts and the element mixture is settled for three hours, so that liquid and solid mixtures can be conveniently obtained, in the step (4), a worker filters and concentrates the clarified liquid to remove salt, ash, water and small molecular impurities in the clarified liquid to obtain a filtrate, in the step (5), the worker decompresses the filtrate through a scraper film, compresses the filtrate at high temperature and high pressure to obtain a concentrated liquid, in the step (6), the worker adds an alkali solution into the concentrated liquid to adjust the pH value to nine to eleven to enable the concentrated liquid to be in a free state, adds an equivalent lipophilic solvent into the concentrated liquid, stirs the mixture at normal temperature for one hour, stands the mixture for layering to obtain an extract, in the step (7), the worker concentrates the extract into a paste, and performs spray drying on the paste extract to obtain an inhibitor, wherein the paste extract in pure water is five to seven times.
In the invention, the working steps of the device are as follows:
the first embodiment is as follows:
when the paste extract in the pure water is four times;
in the step (1), workers prepare and clean herbaceous plants such as mulberries, liquorice, white paeony roots, purple perilla, radix puerariae, Chinese yams and dandelions and prepare active probiotics, vitamins A, B1, B2, B6, B12, folic acid, pantothenic acid, nicotinic acid, C, D, E and calcium, magnesium, zinc, selenium, iron, copper and manganese in advance, the herbaceous plants such as mulberries, liquorice, white paeony roots, purple perilla, radix puerariae, Chinese yams and dandelions are uniformly added with water and are extruded for one hour to obtain extract liquor, various elements are mixed at high temperature to obtain a mixture, in the step (3), the extracts of the herbaceous plants and the element mixture are mixed together by the workers, and the mixture of the various extracts and the element mixture is settled for three hours, so that liquid and solid mixtures can be conveniently obtained, in the step (4), a worker filters and concentrates the clarified liquid to remove salt, ash, water and small molecular impurities in the clarified liquid to obtain a filtrate, in the step (5), the worker decompresses the filtrate by a scraper film, compresses the filtrate at high temperature and high pressure to obtain a concentrated liquid, in the step (6), the worker adds an alkali solution into the concentrated liquid to adjust the pH value to nine to eleven to enable the concentrated liquid to be in a free state, adds an equivalent lipophilic solvent into the concentrated liquid, stirs the mixture at normal temperature for one hour, and stands the mixture for layering to obtain an extract, in the step (7), the effect of the worker in concentrating the extract is hardened, and the inhibitor is difficult to obtain by spray drying the pasty extract.
Example two:
when the pasty extract liquid in the pure water is six times;
in the step (1), workers prepare and clean herbaceous plants such as mulberries, liquorice, white paeony roots, purple perilla, radix puerariae, Chinese yams and dandelions and prepare active probiotics, vitamins A, B1, B2, B6, B12, folic acid, pantothenic acid, nicotinic acid, C, D, E and calcium, magnesium, zinc, selenium, iron, copper and manganese in advance, the herbaceous plants such as mulberries, liquorice, white paeony roots, purple perilla, radix puerariae, Chinese yams and dandelions are uniformly added with water and are extruded for one hour to obtain extract liquor, various elements are mixed at high temperature to obtain a mixture, in the step (3), the extracts of the herbaceous plants and the element mixture are mixed together by the workers, and the mixture of the various extracts and the element mixture is settled for three hours, so that liquid and solid mixtures can be conveniently obtained, in the step (4), a worker filters and concentrates the clarified liquid to remove salt, ash, water and small molecular impurities in the clarified liquid to obtain a filtrate, in the step (5), the worker decompresses the filtrate by a scraper film, compresses the filtrate at high temperature and high pressure to obtain a concentrated liquid, in the step (6), the worker adds an alkali solution into the concentrated liquid to adjust the pH value to nine to eleven to enable the concentrated liquid to be in a free state, adds an equivalent lipophilic solvent into the concentrated liquid, stirs the mixture at normal temperature for one hour, stands the mixture for layering to obtain an extract, in the step (7), the worker concentrates the extract into a paste, and performs spray drying on the paste extract to obtain the inhibitor.
Example three:
when the paste extract in the pure water is eight times;
in the step (1), workers prepare and clean herbaceous plants such as mulberries, liquorice, white paeony roots, purple perilla, radix puerariae, Chinese yams and dandelions and prepare active probiotics, vitamins A, B1, B2, B6, B12, folic acid, pantothenic acid, nicotinic acid, C, D, E and calcium, magnesium, zinc, selenium, iron, copper and manganese in advance, the herbaceous plants such as mulberries, liquorice, white paeony roots, purple perilla, radix puerariae, Chinese yams and dandelions are uniformly added with water and are extruded for one hour to obtain extract liquor, various elements are mixed at high temperature to obtain a mixture, in the step (3), the extracts of the herbaceous plants and the element mixture are mixed together by the workers, and the mixture of the various extracts and the element mixture is settled for three hours, so that liquid and solid mixtures can be conveniently obtained, in the step (4), a worker filters and concentrates the clarified liquid to remove salt, ash, water and small molecular impurities in the clarified liquid to obtain a filtrate, in the step (5), the worker decompresses the filtrate by a scraper film, compresses the filtrate at high temperature and high pressure to obtain a concentrated liquid, in the step (6), the worker adds an alkali solution into the concentrated liquid to adjust the pH value to nine to eleven to enable the concentrated liquid to be in a free state, adds an equivalent lipophilic solvent into the concentrated liquid, stirs the mixture at normal temperature for one hour, and stands the mixture for layering to obtain an extract, in the step (7), the effect of the worker in concentrating the extract is softened, and the inhibitor is difficult to obtain by spray drying the pasty extract.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (8)
1. A method for extracting inhibitor from multielement material, which is characterized by comprising the following steps: the method comprises the following working steps:
preparing herbaceous plants to be extracted in advance;
extracting the cleaned herbs to obtain extract and mixture;
precipitating the extract and the mixture to obtain a clarified liquid;
cleaning the clarified liquid to remove impurities to obtain filtrate;
concentrating the filtrate under high pressure to obtain concentrated solution;
extracting the concentrated solution to obtain an extract;
concentrating and drying the extract to obtain the inhibitor.
2. The method for extracting multi-element substance as inhibitor of claim 1, wherein: in the step (1), the workers prepare and clean herbs such as mulberry, liquorice, white peony root, purple perilla, radix puerariae, Chinese yam and dandelion, prepare active probiotics, vitamins A, B1, B2, B6, B12, folic acid, pantothenic acid, nicotinic acid, C, D, E and calcium, magnesium, zinc, selenium, iron, copper and manganese in advance and prepare the substances in advance.
3. The method of claim 1, wherein the inhibitor is extracted from the multielement material by: in the step (2), workers uniformly add water into the herbs such as the mulberries, the liquorice, the white paeony roots, the purple perilla, the kudzuvine roots, the Chinese yams and the dandelions, extract the herbs for one hour by extrusion to obtain an extract, and mix various elements at a high temperature to obtain a mixture.
4. The method for extracting multi-element substance as inhibitor of claim 1, wherein: in the step (3), the worker mixes the extract of the herb with the element mixture together, and precipitates the mixture of the various extracts and the element mixture for three hours, so that it is convenient to obtain a clarified liquid and a solid mixture.
5. The method of claim 1, wherein the inhibitor is extracted from the multielement material by: in the step (4), the clear liquid is filtered and concentrated by workers, so that salt, ash, water and small molecular impurities are removed from the clear liquid, and a filtrate is obtained.
6. The method of claim 1, wherein the inhibitor is extracted from the multielement material by: in the step (5), the staff carries out scraper film decompression on the filtrate, and then compresses the filtrate at high temperature and high pressure to obtain the concentrated solution.
7. The method for extracting multi-element substance as inhibitor of claim 1, wherein: in the step (6), a worker adds an alkali solution into the concentrated solution to adjust the pH value to nine to eleven so that the concentrated solution is in a free state, adds the same lipophilic solvent into the concentrated solution, stirs the mixture for one hour at normal temperature, and stands the mixture for layering to obtain an extract.
8. The method of claim 1, wherein the inhibitor is extracted from the multielement material by: in the step (7), the worker concentrates the extract liquid into paste, the paste extract liquid is subjected to spray drying to obtain the inhibitor, and the paste extract liquid in pure water is five to seven times.
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