CN114568515B - Preparation method of protein solution containing beta-hydroxy-beta-calcium methylbutyrate - Google Patents
Preparation method of protein solution containing beta-hydroxy-beta-calcium methylbutyrate Download PDFInfo
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- CN114568515B CN114568515B CN202011376224.4A CN202011376224A CN114568515B CN 114568515 B CN114568515 B CN 114568515B CN 202011376224 A CN202011376224 A CN 202011376224A CN 114568515 B CN114568515 B CN 114568515B
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- methylbutyrate
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- 239000011575 calcium Substances 0.000 title claims abstract description 14
- 229910052791 calcium Inorganic materials 0.000 title claims abstract description 14
- JGFBQFKZKSSODQ-UHFFFAOYSA-N Isothiocyanatocyclopropane Chemical compound S=C=NC1CC1 JGFBQFKZKSSODQ-UHFFFAOYSA-N 0.000 title claims abstract description 12
- PWLNAUNEAKQYLH-UHFFFAOYSA-N butyric acid octyl ester Natural products CCCCCCCCOC(=O)CCC PWLNAUNEAKQYLH-UHFFFAOYSA-N 0.000 title claims abstract description 12
- UUIQMZJEGPQKFD-UHFFFAOYSA-N n-butyric acid methyl ester Natural products CCCC(=O)OC UUIQMZJEGPQKFD-UHFFFAOYSA-N 0.000 title claims abstract description 12
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 239000012460 protein solution Substances 0.000 title abstract description 11
- WLJUMPWVUPNXMF-UHFFFAOYSA-L calcium;3-hydroxy-3-methylbutanoate Chemical compound [Ca+2].CC(C)(O)CC([O-])=O.CC(C)(O)CC([O-])=O WLJUMPWVUPNXMF-UHFFFAOYSA-L 0.000 claims abstract description 67
- 239000000243 solution Substances 0.000 claims abstract description 65
- 239000007864 aqueous solution Substances 0.000 claims abstract description 13
- 150000007524 organic acids Chemical class 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims description 47
- 235000013336 milk Nutrition 0.000 claims description 39
- 239000008267 milk Substances 0.000 claims description 39
- 210000004080 milk Anatomy 0.000 claims description 39
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 27
- 239000002994 raw material Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 235000015165 citric acid Nutrition 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 4
- 102000014171 Milk Proteins Human genes 0.000 claims description 4
- 108010011756 Milk Proteins Proteins 0.000 claims description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 4
- 108010046377 Whey Proteins Proteins 0.000 claims description 4
- 102000007544 Whey Proteins Human genes 0.000 claims description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
- 235000021239 milk protein Nutrition 0.000 claims description 4
- 235000020183 skimmed milk Nutrition 0.000 claims description 4
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 2
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 2
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 2
- 239000005862 Whey Substances 0.000 claims description 2
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 2
- 235000010323 ascorbic acid Nutrition 0.000 claims description 2
- 239000011668 ascorbic acid Substances 0.000 claims description 2
- 229960005070 ascorbic acid Drugs 0.000 claims description 2
- 235000013373 food additive Nutrition 0.000 claims description 2
- 239000002778 food additive Substances 0.000 claims description 2
- 239000004310 lactic acid Substances 0.000 claims description 2
- 235000014655 lactic acid Nutrition 0.000 claims description 2
- 239000001630 malic acid Substances 0.000 claims description 2
- 235000011090 malic acid Nutrition 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 2
- 235000002906 tartaric acid Nutrition 0.000 claims description 2
- 239000011975 tartaric acid Substances 0.000 claims description 2
- 235000021119 whey protein Nutrition 0.000 claims description 2
- 235000008939 whole milk Nutrition 0.000 claims description 2
- 239000003623 enhancer Substances 0.000 claims 1
- 235000016709 nutrition Nutrition 0.000 claims 1
- 230000035764 nutrition Effects 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 abstract description 16
- 108090000623 proteins and genes Proteins 0.000 abstract description 16
- 235000008476 powdered milk Nutrition 0.000 description 16
- 235000018102 proteins Nutrition 0.000 description 13
- 241000519995 Stachys sylvatica Species 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 7
- 238000004925 denaturation Methods 0.000 description 6
- 230000036425 denaturation Effects 0.000 description 6
- 230000006920 protein precipitation Effects 0.000 description 5
- 210000003205 muscle Anatomy 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- AXFYFNCPONWUHW-UHFFFAOYSA-N 3-hydroxyisovaleric acid Chemical compound CC(C)(O)CC(O)=O AXFYFNCPONWUHW-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000000463 material Substances 0.000 description 2
- 235000020122 reconstituted milk Nutrition 0.000 description 2
- 208000025978 Athletic injury Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000008934 Muscle Proteins Human genes 0.000 description 1
- 108010074084 Muscle Proteins Proteins 0.000 description 1
- 206010041738 Sports injury Diseases 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 125000001909 leucine group Chemical class [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000014268 sports nutrition Nutrition 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/1522—Inorganic additives, e.g. minerals, trace elements; Chlorination or fluoridation of milk; Organic salts or complexes of metals other than natrium or kalium; Calcium enrichment of milk
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/08—Whey; Whey preparations containing other organic additives, e.g. vegetable or animal products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
- A23J3/08—Dairy proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Inorganic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a preparation method of a protein solution containing beta-hydroxy-beta-calcium methylbutyrate. An aqueous solution of calcium beta-hydroxy-beta-methylbutyrate is prepared, the pH value of the aqueous solution of calcium beta-hydroxy-beta-methylbutyrate is adjusted to 6.5-7.0 by using an organic acid, and then the aqueous solution of calcium beta-hydroxy-beta-methylbutyrate is mixed with a protein-containing solution. The invention lays an excellent foundation for effectively applying beta-hydroxy-beta-methyl calcium butyrate (CaHMB) in the field of protein products, and has simple operation and strong practicability.
Description
Technical Field
The invention relates to the field of protein products, in particular to the field of protein solutions containing beta-hydroxy-beta-calcium methylbutyrate.
Background
The functional raw material of the calcium beta-hydroxy-beta-methylbutyrate is formally obtained as new resource food in China in 2011 (bulletin No. 1 in 2011 of Ministry of health), and the application range of the calcium beta-hydroxy-beta-methylbutyrate comprises sports nutrition food and special medical purpose formula food. Beta-hydroxy-beta-methylbutyric acid (HMB) is a metabolite of leucine. Numerous studies have shown that calcium beta-hydroxy-beta-methylbutyrate (CaHMB) has the effect of preventing muscle decline and increasing muscle protein synthesis. Meanwhile, can regulate muscle synthesis, prevent protein degradation, recover sports injury and the like. The function can be applied to muscle recovery of athletes after exercise and is also very effective for relieving muscle attenuation of middle-aged and elderly people.
However, the problem of protein precipitation occurs when the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) raw material is combined with a protein-containing product, and the application of the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) can be greatly improved by effectively solving the problem.
Disclosure of Invention
Technical problem to be solved
The object of the present invention is to provide a process that is effective in avoiding the precipitation of proteins from the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) starting material when combined with a protein-containing product.
Technical proposal for solving the problems
The present inventors considered that calcium beta-hydroxy-beta-methylbutyrate (CaHMB) exists in an aqueous solution in an ionic form, and calcium bridge is easily formed between protein molecules to cause protein precipitation, and thus considered that an organic acid such as citric acid is added to the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution to be bound to free calcium first, whereby the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) can be prevented from being bound to protein at a subsequent stage to cause protein precipitation.
Specifically, the invention provides a preparation method of a protein solution containing beta-hydroxy-beta-calcium methylbutyrate, which is characterized in that firstly, a beta-hydroxy-beta-calcium methylbutyrate aqueous solution is prepared, the pH value of the beta-hydroxy-beta-calcium methylbutyrate aqueous solution is adjusted to be 6.5-7.0 by using an organic acid, and then the beta-hydroxy-beta-calcium methylbutyrate aqueous solution is mixed with the protein-containing solution.
Preferably, the organic acid is selected from one or more of citric acid, malic acid, lactic acid, tartaric acid and ascorbic acid.
Preferably, the protein-containing solution is a milk-containing protein solution.
Preferably, the milk-containing protein solution is selected from fresh milk and reconstituted milk.
Preferably, the reconstituted milk is formulated with a milk powder which is one or more selected from whole milk powder, skim milk powder, whey protein powder, whey powder, with or without a nutritional supplement or food additive.
In the above production method, the content of the calcium beta-hydroxy-beta-methylbutyrate is preferably 0.1 to 10wt%, preferably 1.0 to 6.0wt%, particularly preferably 2.0 to 3.0wt% relative to the total amount of the milk powder and the calcium beta-hydroxy-beta-methylbutyrate (CaHMB).
In the above production method, preferably, after mixing the aqueous solution of calcium beta-hydroxy-beta-methylbutyrate with the protein solution, the pH of the protein solution containing calcium beta-hydroxy-beta-methylbutyrate is adjusted with an acidity regulator, which is one or more selected from KOH, naOH, sodium bicarbonate.
In the above preparation method, preferably, the pH is adjusted to 6.7 to 6.9.
The invention also provides a preparation method of the milk powder, which is used for preparing the milk protein-containing solution containing the beta-hydroxy-beta-methyl calcium butyrate by using the preparation method, so as to prepare the milk powder.
Preferably, the method comprises the following steps:
step 1: adding the milk powder into warm water at 50 ℃ to prepare milk powder solution;
step 2: adding a raw material of beta-hydroxy-beta-calcium methylbutyrate (CaHMB) into normal-temperature water to prepare a solution of beta-hydroxy-beta-calcium methylbutyrate (CaHMB);
step 3: adding an organic acid or an organic acid and an acidity regulator into the beta-hydroxy-beta-methyl calcium butyrate (CaHMB) solution prepared in the step 2, and regulating the pH value of the beta-hydroxy-beta-methyl calcium butyrate (CaHMB) solution to be 6.5-7.0;
step 4: the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution prepared in step 3 was added to the milk powder solution prepared in step 1.
Advantageous effects
According to the preparation method provided by the invention, protein denaturation does not occur, and denaturation does not occur after boiling.
Drawings
FIG. 1 is a photograph of a milk powder solution containing calcium beta-hydroxy-beta-methylbutyrate (CaHMB) of example 1 of the present invention.
FIG. 2 is a photograph of a milk powder solution containing calcium beta-hydroxy-beta-methylbutyrate (CaHMB) of comparative example 1 of the present invention.
FIG. 3 is a photograph of a milk powder solution containing calcium beta-hydroxy-beta-methylbutyrate (CaHMB) of comparative example 4 of the present invention.
Detailed Description
In the following, specific embodiments of the present invention will be described in more detail for further describing technical features, objects and advantageous effects of the present invention, but the scope of the present invention is not limited thereto.
The raw materials and equipment used in the present invention are common raw materials and equipment in the art unless otherwise specified, and the methods used in the present invention are conventional methods in the art unless otherwise specified.
In the present invention,% means wt% unless otherwise specified.
The raw material of the beta-hydroxy-beta-methylbutyrate calcium (CaHMB) used in the examples and comparative examples of the present invention is provided by Jiangyin technology source pharmaceutical industry Co. The milk powder used was skim milk powder of westpro, new Zealand.
Example 1
Step 1: a milk powder solution was prepared by adding 98g of milk powder to 622g of warm water at 50 ℃.
Step 2: 2g of a raw material of calcium beta-hydroxy-beta-methylbutyrate (CaHMB) was added to 98g of warm water to prepare a 2wt% solution of calcium beta-hydroxy-beta-methylbutyrate (CaHMB), which was stirred well until completely dissolved.
Step 3: to the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution prepared in step 2, a 1wt% citric acid solution was added to adjust the pH of the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution to 6.8.
Step 4: the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution prepared in step 3 was added to the milk powder solution prepared in step 1.
The content of calcium beta-hydroxy-beta-methylbutyrate (CaHMB) in the resulting powdered milk solution was 2% by weight relative to the total amount of powdered milk and calcium beta-hydroxy-beta-methylbutyrate (CaHMB).
The powdered milk solution of step 4 was left at 50℃and observed, and as a result, no denatured white spots were seen over 10 minutes.
The above-mentioned powdered milk solution was further boiled for 3 minutes, left at 50℃and observed, and as a result, no denatured white spots were observed after 10 minutes. The experimental results are shown in fig. 1.
Example 2
Step 1: a milk powder solution was prepared by adding 98g of milk powder to 622g of warm water at 50 ℃.
Step 2: 2g of a raw material of calcium beta-hydroxy-beta-methylbutyrate (CaHMB) was added to 98g of warm water to prepare a 2wt% solution of calcium beta-hydroxy-beta-methylbutyrate (CaHMB), which was stirred well until completely dissolved.
Step 3: to the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution prepared in step 2, a 1wt% citric acid solution was added to adjust the pH of the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution to 6.5.
Step 4: the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution prepared in step 3 was added to the powdered milk solution prepared in step 1, and the pH of the powdered milk solution containing calcium beta-hydroxy-beta-methylbutyrate (CaHMB) was adjusted to 6.7 with 10wt% KOH solution.
The content of calcium beta-hydroxy-beta-methylbutyrate (CaHMB) in the resulting powdered milk solution was 2% by weight relative to the total amount of powdered milk and calcium beta-hydroxy-beta-methylbutyrate (CaHMB).
The powdered milk solution of step 4 was left at 50℃and observed, and as a result, no denatured white spots were seen over 10 minutes.
The above-mentioned powdered milk solution was further boiled for 3 minutes, left at 50℃and observed, and as a result, no denatured white spots were observed after 10 minutes.
Example 3
Step 1: 90g of the powdered milk was added to 630g of warm water at 50℃to prepare a powdered milk solution.
Step 2: 10g of a raw material of calcium beta-hydroxy-beta-methylbutyrate (CaHMB) was added to 90g of warm water to prepare a 10wt% solution of calcium beta-hydroxy-beta-methylbutyrate (CaHMB), which was stirred well until completely dissolved.
Step 3: to the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution prepared in step 2, a 1wt% citric acid solution was added to adjust the pH of the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution to 6.8.
Step 4: the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution prepared in step 3 was added to the milk powder solution prepared in step 1.
The content of calcium beta-hydroxy-beta-methylbutyrate (CaHMB) in the resulting powdered milk solution was 10% by weight relative to the total amount of powdered milk and calcium beta-hydroxy-beta-methylbutyrate (CaHMB).
The powdered milk solution of step 4 was left at 50℃and observed, and as a result, no denatured white spots were seen over 10 minutes.
The above-mentioned powdered milk solution was further boiled for 3 minutes, left at 50℃and observed, and as a result, no denatured white spots were observed after 10 minutes.
Comparative example 1
In example 1, step 3 was omitted, i.e. the pH of the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution was not adjusted with citric acid solution. The calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution prepared in step 2 was directly added to the milk powder solution prepared in step 1.
After being left at 50℃and observed, denatured white spots were observed for 1 minute. As shown in fig. 2.
Comparative example 2
In step 3 of example 1, a 1wt% citric acid solution was added to the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution prepared in step 2, and the pH of the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution was adjusted to 7.1. The remaining steps are the same.
After being left at 50℃and observed, denatured white spots were observed for 1 minute.
Comparative example 3
In step 3 of example 1, a 1wt% citric acid solution was added to the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution prepared in step 2, and the pH of the calcium beta-hydroxy-beta-methylbutyrate (CaHMB) solution was adjusted to 6.3. The remaining steps are the same.
After being left at 50℃and observed, denatured white spots were observed for 1 minute.
Comparative example 4
1.0g, 2.0g, 4.0g and 6.0g of beta-hydroxy-beta-methyl calcium butyrate (CaHMB) are respectively and uniformly mixed with 99g, 98g, 96g and 94g of skim milk powder to prepare 100g dry-mixed materials, and then the dry-mixed materials are respectively added into 720g warm water with the temperature of 50 ℃ to prepare 4 groups of milk powder solutions.
The solution was left at 50℃for 1 minute and the state of the solution was observed. As a result, as the amount of the raw material of calcium β -hydroxy- β -methylbutyrate (CaHMB) added increases, the more serious the occurrence of protein denaturation, particularly, the case where the content of calcium β -hydroxy- β -methylbutyrate (CaHMB) is 6wt% relative to the total amount of the milk powder and calcium β -hydroxy- β -methylbutyrate (CaHMB), the more the denatured white spots are, as shown in fig. 3.
From the above examples, the problem of protein precipitation caused by the combination of beta-hydroxy-beta-methylbutyrate calcium (CaHMB) and protein can be effectively solved by the method of the invention, because the protein denaturation condition does not occur when the beta-hydroxy-beta-methylbutyrate calcium (CaHMB) raw material is added into a protein solution after the pH is adjusted to be within the preferred range of the invention and the protein denaturation condition does not occur after the raw material is boiled. In particular, even if the content of the beta-hydroxy-beta-methylbutyrate calcium (CaHMB) is increased to 10wt% relative to the total amount of the milk powder and the beta-hydroxy-beta-methylbutyrate calcium (CaHMB), the precipitation problem can be well controlled.
Possibility of industrial application
According to the method of the present invention, a protein solution containing calcium beta-hydroxy-beta-methylbutyrate (CaHMB) with stable properties can be obtained. The invention lays an excellent foundation for effectively applying beta-hydroxy-beta-methyl calcium butyrate (CaHMB) in the field of protein products, and has simple operation and strong practicability.
In the field of wet processing of milk powder, the method can be used for adding the raw material of the beta-hydroxy-beta-methyl calcium butyrate (CaHMB) by a wet method, and meanwhile, the protein is ensured not to undergo a denaturation reaction after the feed liquid is subjected to high-temperature powder spraying, and the brewing property is improved to a certain extent.
Claims (5)
1. A process for producing a milk powder, characterized in that a milk protein-containing solution containing calcium beta-hydroxy-beta-methylbutyrate is produced by the following process to produce a milk powder,
the preparation method of the milk protein-containing solution containing the beta-hydroxy-beta-methyl calcium butyrate comprises the following steps in sequence:
step 1: adding milk powder into warm water at 50 ℃ to prepare milk powder solution, wherein the milk powder is one or more selected from whole milk powder, skimmed milk powder, whey protein powder and whey powder, and nutrition enhancer or food additive is added or not added;
step 2: adding a raw material of beta-hydroxy-beta-methyl calcium butyrate into normal-temperature water to prepare a beta-hydroxy-beta-methyl calcium butyrate aqueous solution;
step 3: adding an organic acid into the beta-hydroxy-beta-calcium methylbutyrate aqueous solution prepared in the step 2, and adjusting the pH value of the beta-hydroxy-beta-calcium methylbutyrate aqueous solution to 6.7-6.9, wherein the organic acid is one or more selected from citric acid, malic acid, lactic acid, tartaric acid and ascorbic acid;
step 4: the aqueous solution of the beta-hydroxy-beta-methylbutyrate calcium prepared in the step 3 is added into the milk powder solution prepared in the step 1.
2. The method for producing a milk powder according to claim 1, wherein the content of the calcium beta-hydroxy-beta-methylbutyrate is 0.1 to 10wt% relative to the total amount of the milk powder and the calcium beta-hydroxy-beta-methylbutyrate.
3. The method for producing a milk powder according to claim 2, wherein the content of the calcium beta-hydroxy-beta-methylbutyrate is 1.0 to 6.0wt% relative to the total amount of the milk powder and the calcium beta-hydroxy-beta-methylbutyrate.
4. The method for producing a milk powder according to claim 2, wherein the content of the calcium beta-hydroxy-beta-methylbutyrate is 2.0 to 3.0wt% relative to the total amount of the milk powder and the calcium beta-hydroxy-beta-methylbutyrate.
5. The method for producing a milk powder according to claim 1, wherein after mixing the aqueous solution of calcium beta-hydroxy-beta-methylbutyrate with the milk powder solution, the pH of the milk protein-containing solution containing calcium beta-hydroxy-beta-methylbutyrate is adjusted to 6.7 to 6.9 with an acidity regulator, which is one or more selected from KOH, naOH, sodium bicarbonate.
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| CN109393493A (en) * | 2018-12-17 | 2019-03-01 | 广东君悦营养医学有限公司 | A kind of predigestion type pancebrin of the calcium containing HMB and preparation method thereof |
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