CN114560569A - Application of aerobic denitrification composite bacterial agent in enhanced denitrification in improved A/O process - Google Patents

Application of aerobic denitrification composite bacterial agent in enhanced denitrification in improved A/O process Download PDF

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CN114560569A
CN114560569A CN202210361200.4A CN202210361200A CN114560569A CN 114560569 A CN114560569 A CN 114560569A CN 202210361200 A CN202210361200 A CN 202210361200A CN 114560569 A CN114560569 A CN 114560569A
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denitrification
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pseudomonas
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胡智泉
李炳堂
景方圆
刘冬啟
肖波
程龙
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Huazhong University of Science and Technology
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Abstract

The invention discloses an application of an aerobic denitrification complex bacterial agent in the enhanced denitrification in an improved A/O process, and provides a pseudomonas AD-1 strain to NO3 ‑the-N has stronger degradation performance, and the acinetobacter AD-5 strain has NH resistance4 +N has stronger degradation performance, and the pseudomonas Z1 strain has NO2 ‑The degrading performance of the-N is stronger, three strains of bacteria with different nitrogen metabolism characteristics generate synergistic effect in the process of synchronous nitrification and denitrification, the complementation on the denitrification function is formed, and the composite performance is improvedThe metabolic performance of the flora on the mixed nitrogen source. The invention adds the composite bacteria biofilm filler into an improved A/O reactor with the reflux ratio of the mixed solution of 50 percent, and after 50 days of biological enhancement, COD, TN and PO are carried out4 3‑The removal rate of P is respectively improved by 3.94%, 20.93% and 7.41% compared with that before the P is added; the denitrification efficiency of the synchronous nitrification and denitrification is improved by 28.34 percent compared with that before the addition, the denitrification efficiency of the synchronous nitrification and denitrification is improved by 35.69 percent compared with that before the reflux ratio of the mixed liquid is reduced, and the electric energy consumed by the reflux pump can be reduced by about 60 percent by reducing the reflux ratio of the mixed liquid.

Description

Application of aerobic denitrification composite bacterial agent in enhanced denitrification in improved A/O process
Technical Field
The invention relates to the technical field of wastewater treatment, in particular to application of aerobic denitrification composite bacteria in the enhanced denitrification of an improved A/O (anaerobic/anoxic/oxic) process.
Background
An anoxic/aerobic (A/O) process for treating sewage by activated sludge is composed of front anoxic pool (A pool) and back aerobic pool (O pool), and features that the dissolved oxygen concentration in O pool is maintained at 2-4mg/L and the DO in A pool is controlled below 0.5mg/L, and the water is NH fed in4 +Conversion of-N to NO by O-pool nitrifying bacteria3 -N, refluxing the mixed liquor to remove a large amount of NO in the O pool3 -N is carried to the A pool and is converted into N under the action of denitrifying bacteria in the A pool2And removing, and simultaneously consuming a large amount of organic matters through denitrification, wherein the incompletely degraded organic matters are fully absorbed by heterotrophic bacteria in the O pond.
The A/O denitrification process has the advantages of simple flow and capability of effectively utilizing a carbon source in raw water, but is limited by a traditional biological denitrification mechanism, so that the A/O process has the following problems, and the high efficiency and the economy of denitrification of the A/O process are greatly influenced.
(1) The nitrifying bacteria have low self-productivity and long generation time, and the autotrophic nitrifying bacteria are less dominant to the absorption and utilization of organic matters in the sewage as heterotrophic bacteria, so that higher biological concentration is difficult to maintain and the nitrification effect is poor;
(2) the nitration reaction needs to be carried out under the condition of high-concentration dissolved oxygen (2-4mg/L), while the denitrification reaction needs a low-concentration dissolved oxygen (0-0.5mg/L) environment, so that the nitration and denitrification processes need to be carried out in different reaction tanks, and the floor area and the operation cost are increased;
(3) the A/O system must obtain good denitrification efficiency through the backflow of nitrifying liquid, and power consumption and operating cost are increased; in addition, a large amount of dissolved oxygen is brought into the pool A in the backflow process, so that the fluctuation of the dissolved oxygen in the pool A is large, the activity of denitrifying bacteria is inhibited, and the denitrifying efficiency is influenced;
(4) acidity generated in the nitration process and alkalinity generated in the denitrification process need to be neutralized by adding medicaments respectively, so that the operation cost is increased, and secondary pollution of effluent water can be caused due to the problem of excessive medicament addition.
Therefore, aiming at the problems of low denitrification efficiency and high energy consumption of the traditional A/O process, a new biological denitrification technology needs to be sought so as to fundamentally solve the problems.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide the application of the aerobic denitrification composite bacteria agent in the improved A/O process for enhancing denitrification, solve the technical problem of low denitrification efficiency of the traditional A/O process and achieve the aims of reducing the sewage reflux ratio and saving energy consumption.
In order to achieve the purpose, the invention adopts the following technical scheme:
the application of the aerobic denitrification composite bacterial agent in the improved A/O process for enhanced denitrification is characterized by comprising the following specific steps:
(1) adding the nitrohair bulbs and the composite microbial inoculum into the enrichment culture solution for culturing and biofilm formation, and when the surface of the filler is in a light yellow color and obvious floccules appear, indicating that the filler biofilm formation is finished;
(2) the filler after film formation is placed in an aerobic zone 2 of the improved A/O process, the reflux ratio of the mixed solution is gradually reduced to 50%, and after the reactor stably operates, the application of the aerobic denitrification composite microbial inoculum in the improved A/O process is realized;
the compound microbial inoculum is prepared by compounding three bacterium solutions of pseudomonas AD-1, acinetobacter AD-5 and pseudomonas Z1;
the improved A/O process comprises an anoxic zone, an aerobic zone 1 and an aerobic zone 2, and mixed liquor flows back to the anoxic zone from the aerobic zone 2.
Preferably, in the step (1), the volume ratio of the nitrification hair bulb, the complex microbial inoculum and the enrichment culture solution is 1-2L: 5-10 mL: 2-4L.
Preferably, the volume ratio of the bacteria liquid of the pseudomonas AD-1, the acinetobacter AD-5 and the pseudomonas Z1 is 1-2:1-3:1-2, and the bacterial density OD of each bacteria liquid600The values were all 1.0.
Preferably, the preparation method of the complex microbial inoculum comprises the following steps: respectively inoculating a pseudomonas AD-1 strain, an acinetobacter AD-5 strain and a pseudomonas Z1 strain into a sterilized LB culture medium by using inoculating loops, then putting the inoculated bacteria into a constant-temperature shaking bed for culture, centrifugally collecting bacteria after the culture is finished, diluting with sterile water to a constant volume to respectively obtain 3 bacteria solutions, and then mixing the 3 bacteria solutions to obtain the compound microbial inoculum.
Preferably, activated sludge is added into the aerobic zone 1, and biofilm culturing filler is added into the aerobic zone 2.
Preferably, the concentration of the dissolved oxygen in the aerobic zone 1 is set to 2-4mg/L, and the concentration of the dissolved oxygen in the aerobic zone 2 is set to 1-2 mg/L.
Preferably, the volume ratio of the anoxic zone, the aerobic zone 1 and the aerobic zone 2 is 2:1: 1.
Preferably, in step (2), the adjustment of the reflux ratio of the mixed liquid is divided into the following four stages:
stage I: adjusting the reflux ratio of the mixed solution from 200% to 150%, keeping other operation conditions unchanged, and detecting NH in effluent of the anoxic zone and the aerobic zone every day4 +-N、NO3 --N、NO2 --N、PO4 3-P, COD concentration until the reactor is operating steadily and detecting MLSS, SVI in aerobic zone 1 after stabilization;
stage II: under the condition of stable operation of the reactor, the reflux ratio of the mixed solution is adjusted from 200 percent to 150 percent, and other operations are performed in the same phase I;
and stage III: under the condition of stable operation of the reactor, the reflux ratio of the mixed solution is adjusted from 150 percent to 100 percent, and other operations are performed in the same phase I;
stage IV: under the condition of stable operation of the reactor, the reflux ratio of the mixed liquid is adjusted from 100% to 50%, and the other operations are the same as the stage I.
Compared with the prior art, the invention has the following beneficial effects:
the pseudomonas AD-1 strain provided by the invention is NO3 -the-N has stronger degradation performance, and the acinetobacter AD-5 strain has NH resistance4 +N has stronger degradation performance, and the pseudomonas Z1 strain has NO2 -The three bacteria have the advantages that the three bacteria utilize ecological niche difference, exert respective denitrification advantages and improve the metabolic performance of the composite flora on a mixed nitrogen source. In the invention, the mixed liquid flows back from the aerobic zone 2, the concentration of dissolved oxygen in the aerobic zone 2 is lower, and the concentration of dissolved oxygen in the anoxic tank is not increased after the mixed liquid flows back, thereby being beneficial to the control of the dissolved oxygen in the section A.
The invention adds the composite bacteria biofilm culturing filler into an improved A/O reactor with the reflux ratio of mixed liquor of 50 percent, and after 50 days of biological enhancement, COD, TN and PO are added4 3-The removal rate of P is respectively improved by 3.94%, 20.93% and 7.41% compared with that before the P is added; the denitrification efficiency of the synchronous nitrification and denitrification is improved by 28.34 percent compared with that before the addition, the denitrification efficiency of the synchronous nitrification and denitrification is improved by 35.69 percent compared with that before the reflux ratio of the mixed liquid is reduced, and the electric energy consumed by the reflux pump can be reduced by about 60 percent by reducing the reflux ratio of the mixed liquid.
Drawings
FIG. 1 is a scanning electron microscope image of Pseudomonas AD-1 strain provided by the invention;
FIG. 2 is a scanning electron micrograph of Acinetobacter AD-5 strain provided by the present invention;
FIG. 3 is a diagram of the synchronous nitrification and denitrification characteristics of the composite microbial inoculum provided by the invention;
FIG. 4 is a diagram of the synchronous nitrification and denitrification characteristics of Pseudomonas AD-1 provided by the invention;
FIG. 5 is a graph showing the simultaneous nitrification and denitrification characteristics of Acinetobacter AD-5 provided by the present invention;
FIG. 6 is a diagram of the synchronous nitrification and denitrification characteristics of Pseudomonas Z1 provided by the invention;
FIG. 7 is a flow chart of denitrification pathway of the complex microbial inoculum provided by the invention;
FIG. 8 is a process flow chart of the application of the aerobic denitrification complex bacterial agent in the improved A/O process for enhanced denitrification;
FIG. 9 shows NH after reducing reflux ratio of mixed solution and adding biofilm formation filler4 +-N、NO2 --N、NO3 -N Water in and out concentration variations.
Detailed Description
The present invention will be described in more detail with reference to specific preferred embodiments, but the present invention is not limited to the following embodiments.
It should be noted that, unless otherwise specified, the chemical reagents involved in the present invention are commercially available.
The pseudomonas AD-1 strain (Genbank accession number: MW426198), the acinetobacter AD-5 strain (Genbank accession number: MW426203) and the pseudomonas Z1 strain (Genbank accession number: MT898541) in the invention are all derived from activated sludge, and are obtained by domesticating, screening and separating the sludge, and the strain screening and purifying specifically comprises the following steps:
(1) enrichment: uniformly mixing 10mL of activated sludge and 90mL of sterile water, putting 1mL of sludge suspension into an enrichment medium, and carrying out shaking culture in a constant-temperature oscillator at 30 ℃ for 3d at 120 r/min;
(2) domestication: taking 1ml of the enriched bacterial liquid, transferring the enriched bacterial liquid to a liquid acclimation culture medium for continuous culture, and respectively diluting bacterial suspensions to 10 after 24 hours4、105、106、107Taking 0.1mL of the culture medium, coating the culture medium on a solid domestication culture medium, and carrying out inverted culture in a constant temperature incubator at 30 ℃ for 3d until single colonies with clear forms grow on the surface of a flat plate; selecting single colonies of different forms, inoculating in liquid acclimation culture medium, culturing overnight in constant temperature oscillator at 30 deg.C and 120r/min, and culturing to logarithmic growthGrowth period (OD)6001.0), and repeating the experimental operation for 3 times;
(3) screening: picking single colonies with different forms from the domesticated culture medium diluted and coated for 3 times, respectively inoculating into liquid domesticated culture medium, and performing constant temperature shaking culture at 30 deg.C and 120r/min for 24 h; the bacterial solution was diluted in a gradient (10)-4-10-7) Coating on a BTB culture medium, culturing in a constant temperature incubator at 30 ℃ for 3d, selecting a single colony which is changed from yellow to blue in the culture medium, continuously streaking and purifying on the BTB culture medium, and repeating the steps for 4 times;
(4) after multiple times of enrichment, separation and purification, strains with heterotrophic nitrification or aerobic denitrification performance are screened out, the screened strains are respectively inoculated into a heterotrophic nitrification identification culture medium and an aerobic denitrification identification culture medium according to the inoculum concentration of 2 percent, after shaking cultivation is carried out for 24 hours at the temperature of 30 ℃ and at the speed of 120r/min, strains with the heterotrophic nitrification or aerobic denitrification TN removal rate higher than 80 percent are reserved according to the water quality detection index result, and 3 high-efficiency aerobic denitrification strains are screened out and named as pseudomonas AD-1, acinetobacter AD-5 and pseudomonas Z1;
wherein the formula of the enrichment medium is as follows: 5g of peptone, 5g of yeast extract, 10g of NaCl and 1L of distilled water;
the formula of the domestication culture medium is as follows: CH (CH)3COONa 2.93g，KNO3 0.722g，KH2PO4 0.088g，MgSO4·7H20.2g of O, 2mL of trace elements and 1L of distilled water; the formula of the trace elements is as follows: EDTA 15g, ZnSO4 0.2g，MnCl2·4H2O 1.5g，FeSO4·7H2O 0.5g，CuSO4·5H2O 0.5g，CoCl2·6H2O 0.3g，Na2MoO4·2H2O 0.2g，CaCl20.1g, 1L of distilled water;
the BTB culture medium formula is as follows: CH (CH)3COONa 2.93g，KNO3 0.722g，KH2PO4 0.088g，MgSO4·7H20.2g of O, 2mL of trace elements, 2% of agar, 1% of BTB and 1L of distilled water; the formula of the trace elements is as follows: EDTA 15g, ZnSO4 0.2g，MnCl2·4H2O 1.5g，FeSO4·7H2O 0.5g，CuSO4·5H2O 0.5g，CoCl2·6H2O 0.3g，Na2MoO4·2H2O 0.2g，CaCl20.1g, 1L of distilled water;
the aerobic nitrification identification culture medium comprises the following components: CH (CH)3COONa 0.937g，KNO3 0.288g，KH2PO40.0351g，MgSO47H2O 0.2.2 g, trace elements 2mL, 1L of distilled water; the formula of the trace elements is as follows: EDTA 15g, ZnSO4 0.2g，MnCl2·4H2O 1.5g，FeSO4·7H2O 0.5g，CuSO4·5H2O0.5g，CoCl2·6H2O 0.3g，Na2MoO4·2H2O 0.2g，CaCl20.1g, 1L of distilled water;
the formula of the heterotrophic nitrification identification culture medium is as follows: CH (CH)3COONa 0.937g，NH4Cl 0.153g，KH2PO40.0351g，MgSO4·7H20.2g of O, 2mL of trace elements and 1L of distilled water; the formula of the trace elements is as follows: EDTA 15g, ZnSO4 0.2g，MnCl2·4H2O 1.5g，FeSO4·7H2O 0.5g，CuSO4·5H2O0.5g，CoCl2·6H2O 0.3g，Na2MoO4·2H2O 0.2g，CaCl20.1g, 1L of distilled water;
the Pseudomonas (Pseudomonas sp.) AD-1 and the Pseudomonas (Pseudomonas sp.) Z1 respectively have 100 percent of homology with Bacterium strain GZ16111700525(Genbank accession number: KY963532.1) and Pseudomonas entomophila L48(Genbank accession number: PRJNA16800) in a gene bank, belong to discovered strains, and are not preserved;
acinetobacter Acinetobacter sp.AD-5, which is a new species, is preserved in China center for type culture Collection (CCTCC for short, address No. 299 of eight branches in Wuchang district, Wuhan city, Hubei province) at 12 months and 21 days in 2021, and the preservation number is: CCTCC M20211654.
Example 1
Will be falseRespectively enriching the monad AD-1 strain, the acinetobacter AD-5 strain and the pseudomonas Z1 strain by using LB culture medium and then diluting to OD600The value is 1, then the aerobic denitrifying composite bacteria are obtained by mixing the aerobic denitrifying composite bacteria according to the volume ratio of 1:1:1, then the composite bacteria are inoculated into 100mL of synchronous nitrification and denitrification culture medium according to the inoculation amount (v/v) of 2 percent, and NH is added4 +-N and NO3 -Culturing N-N as mixed nitrogen source on a shaker at 30 deg.C and 120r/min for 24h, and detecting OD in water600、NH4 +-N、NO3 --N、NO2 -Concentration changes of water quality indexes such as N and TN, wherein the LB culture medium formula is as follows: 10g of peptone, 5g of yeast extract, 5g of NaCl and 1L of distilled water.
Comparative example 1
Enriching pseudomonas AD-1 strain by LB culture medium and diluting to OD600The value is 1, then the culture medium is inoculated in 100mL of synchronous nitrification and denitrification culture medium in an inoculation amount (v/v) of 2 percent, and NH is used4 +-N and NO3 -Culturing N-N as mixed nitrogen source on a shaker at 30 deg.C and 120r/min for 24h, and detecting OD in water600、NH4 +-N、NO3 --N、NO2 -Concentration changes of water quality indexes such as-N and TN, wherein the LB culture medium formula is as follows: 10g of peptone, 5g of yeast extract, 5g of NaCl and 1L of distilled water.
Comparative example 2
Enriching acinetobacter AD-5 strain with LB culture medium and diluting to OD600The value is 1, then the culture medium is inoculated in 100mL of synchronous nitrification and denitrification culture medium in an inoculation amount (v/v) of 2 percent, and NH is used4 +-N and NO3 -Culturing N-N as mixed nitrogen source on a shaker at 30 deg.C and 120r/min for 24h, and detecting OD in water600、NH4 +-N、NO3 --N、NO2 -Concentration changes of water quality indexes such as-N and TN; wherein the LB culture medium formula is as follows: 10g of peptone, 5g of yeast extract, 5g of NaCl and 1L of distilled water.
Comparative example 3
The pseudomonas Z1 strain is enriched by LB culture medium and diluted toOD600The value is 1, then the culture medium is inoculated in 100mL of synchronous nitrification and denitrification culture medium in an inoculation amount (v/v) of 2 percent, and NH is used4 +-N and NO3 -Culturing N-N as mixed nitrogen source on a shaker at 30 deg.C and 120r/min for 24h, and detecting OD in water600、NH4 +-N、NO3 --N、NO2 -Concentration changes of water quality indexes such as-N and TN; wherein the LB culture medium formula is as follows: 10g of peptone, 5g of yeast extract, 5g of NaCl and 1L of distilled water.
The experimental results are shown in figures 3-6, and it can be seen from the figures that each single bacterium and compound bacterium agent show different degradation characteristics to different nitrogen sources in the process of synchronous nitrification and denitrification, and AD-1 shows different degradation characteristics to NO3 -the-N has stronger degradation performance, and NO is generated after 24 hours of culture3 -N is reduced to 0, but NH4 +-N and NO2 -The residual quantity of N is higher and is respectively 4.12mg/L and 2.62 mg/L; AD-5 showed strong heterotrophic nitrification capacity, NH after 24h of culture4 +N degraded to 0.56mg/L but with higher NO2 --N and NO3 --N accumulation, 3.10mg/L and 2.70mg/L, respectively; z1 for NO2 -Strong degradation property of-N, NO NO after 24h of culture2 -Accumulation of-N, NH4 +-N and NO3 --N accumulation amounts of 4.22mg/L and 1.18mg/L, respectively; and the combination AD-1+ AD-5+ Z1 vs NH4 +-N、NO2 --N and NO3 -N has strong degradation capability, the accumulation amount of various nitrogen elements is less than 1mg/L after 24 hours of culture, simultaneously, the growth and denitrification efficiency of the composite microbial inoculum in the mixed nitrogen source are higher than that of single bacteria, which is probably because three strains of bacteria with different nitrogen metabolism characteristics generate synergistic effect in the synchronous nitrification and denitrification process, complementation is formed on denitrification function, a high-efficiency and complete nitrogen metabolism path is formed through interaction among the strains, as shown in figure 7, a bent arrow shows the main degradation path of the flora to the mixed nitrogen source, a dotted arrow shows the possible degradation path of part of the nitrogen source, and AD-1, AD-5 and Z1 utilize ecology to realize the degradation of the nitrogen sourceThe position difference gives play to respective denitrification advantages and improves the metabolic performance of the composite flora on the mixed nitrogen source.
An application of an aerobic denitrification complex microbial inoculum in an improved A/O process for enhancing denitrification is shown in a process flow chart of figure 8, and comprises the following specific steps:
the experimental wastewater is used for simulating urban domestic sewage, and the specific pollutant components and contents are as follows: COD 300mg/L, TN 40mg/L, NH4 +-N is 40mg/L, TP is 8mg/L, MgSO4·7H2O is 0.06mg/L, anhydrous CaCl2The content of the nitrified wool balls is 0.02mg/L, the nitrified wool balls are used as biological fillers in experiments, the biological fillers are purchased from environmental protection technology limited company of Longxin in Henan, the fillers are divided into an inner layer sphere and an outer layer sphere, the inner part of the filler is a wool ball with the diameter of 30mm, the materials are polyester hollow fibers, the outer shell of the filler is a hollowed polypropylene reticular sphere, and the biological fillers have the advantages of light weight, large specific surface area, high porosity, strong biological adhesion and the like.
Adding 2.5L of enrichment culture solution and 1.5L of nitrated hair bulb into beaker, and then adding 3 bacterial liquid (OD)6001) inoculating 2mL of each seed into a beaker for culture, wherein the components and the content of an enrichment culture solution are (g/L): CH (CH)3COONa 5g，NH4Cl 0.306g，KNO3 0.576g，KH2PO4 0.02g，MgSO4·7H20.2g of O and 2mL of trace elements, wherein the two beakers are subjected to intermittent aeration during the culture period, DO in the beakers is maintained at 1mg/L, the water temperature is 25 ℃, one period is 3d, the culture solution is replaced after each period is finished, the surface color of the filler is regularly observed, and when the surface of the carrier is in a light yellow color and obvious floccules appear, the film hanging is basically finished;
taking simulated domestic sewage as inlet water, inoculating activated sludge into an improved A/O reactor, controlling the MLSS concentration in the reactor to be 3g/L, discharging sludge periodically, wherein the sludge reflux ratio is 100%, the reflux ratio of mixed liquor is gradually reduced from 200% to 50%, continuously operating the reactor, and detecting NH in outlet water of a secondary sedimentation tank every day4 +-N、NO3 --N、NO2 --N、PO4 3-P, COD concentration until the reactor is operating steadily;
improved stable operation of A/O processThen, the sludge in the aerobic zone 2 is discharged, 25 percent of film-forming filler in volume ratio is added, the concentration of dissolved oxygen is reduced from 4mg/L to 2mg/L, other operation conditions are kept unchanged, and NH in the effluent water of each biological pond is detected every day4 +、NO3 --N、NO2 --N、PO4 3--P, TN, COD concentration until the reactor is stably operated, and detecting MLSS, SVI in the aerobic zone 1 after stabilization.
As shown in FIG. 9, it can be seen that NO in effluent decreased as the reflux ratio of the mixed solution decreased3 -The increasing concentration of-N and TN is mainly due to the higher amount of NO3 --N was not subjected to reflux denitrification. After the film forming filler is added, discharging NO3 -The initial increase in N concentration may be due to the filler microorganisms not yet adapting to the complex community environment in aerobic zone 2 and competing with indigenous microorganisms for the substrate, the enhanced microorganisms gradually exhibiting excellent aerobic denitrification with increasing enhancement time, and the NO concentration in the effluent on day 8 of the biofilm-formation filler input3 -The concentration of-N is obviously reduced, the removal efficiency of TN is greatly improved, and then NO is added3 -The rising trend of the N removal efficiency is gradually slowed down and even reduced, because part of the enhanced microorganisms on the filler are gradually replaced by the dominant indigenous flora in the system, the content of exogenous enhanced microorganisms in the system is gradually reduced, so that the aerobic denitrification efficiency is reduced, the TN removal rate gradually tends to be stable about 30 days of enhanced denitrification, so that a relatively stable flora structure is formed in the system, in addition, in the influence of the exogenous enhanced flora and the long-term operation process of the system, autotrophic nitrifying bacteria, heterotrophic nitrifying bacteria, anoxic denitrification and aerobic denitrification bacteria in different proportions in the reactor can form a new synchronous nitrification denitrification system, the stable and rich functional flora structure ensures the efficient denitrification performance of the system, and the effluent quality is obviously improved after 50 days of enhanced denitrification, this shows that the microorganisms in the biofilm culturing filler improve the denitrification efficiency of the system through the aerobic denitrification.
Finally, it is to be noted that: the above examples do not limit the invention in any way. It will be apparent to those skilled in the art that various modifications and improvements can be made to the present invention. Accordingly, any modification or improvement made without departing from the spirit of the present invention is within the scope of the claimed invention.

Claims (7)

1. The application of the aerobic denitrification composite bacterial agent in the improved A/O process for enhanced denitrification is characterized by comprising the following specific steps:
(1) adding the nitrohair bulbs and the composite microbial inoculum into the enrichment culture solution for culturing and biofilm formation, and when the surface of the filler is in a light yellow color and obvious floccules appear, indicating that the filler biofilm formation is finished;
(2) the filler after film formation is placed in an aerobic zone 2 of the improved A/O process, the reflux ratio of the mixed solution is gradually reduced to 50%, and after the reactor stably operates, the application of the aerobic denitrification composite microbial inoculum in the improved A/O process is realized;
the compound microbial inoculum is compounded by three bacterial liquids of pseudomonas AD-1, acinetobacter AD-5 and pseudomonas Z1;
the improved A/O process comprises an anoxic zone, an aerobic zone 1 and an aerobic zone 2, and mixed liquor flows back to the anoxic zone from the aerobic zone 2.
2. The application of the aerobic denitrification complex bacterial agent in the improved A/O process for enhancing denitrification as claimed in claim 1, wherein in the step (1), the volume ratio of the nitrification hair bulb, the complex bacterial agent and the enrichment culture solution is 1-2L: 5-10 mL: 2-4L.
3. The application of the aerobic denitrification complex bacterial agent in the improved A/O process for enhanced denitrification according to claim 1, wherein the volume ratio of the bacterial liquids of the pseudomonas AD-1, the acinetobacter AD-5 and the pseudomonas Z1 is 1-2:1-3:1-2, and the bacterial density OD of each bacterial liquid600The values were all 1.0.
4. The application of the aerobic denitrification composite bacterial agent in the improved A/O process for enhanced denitrification according to claim 1, wherein the preparation method of the composite bacterial agent comprises the following steps: respectively inoculating a pseudomonas AD-1 strain, an acinetobacter AD-5 strain and a pseudomonas Z1 strain into a sterilized LB culture medium by using inoculating loops, then putting the inoculated bacteria into a constant-temperature shaking bed for culture, centrifugally collecting bacteria after the culture is finished, diluting with sterile water to a constant volume to respectively obtain 3 bacteria solutions, and then mixing the 3 bacteria solutions to obtain the compound microbial inoculum.
5. The application of the aerobic denitrification complex bacterial agent in the improved A/O process for enhanced denitrification according to claim 1, wherein activated sludge is added into the aerobic zone 1, and biofilm culturing filler is added into the aerobic zone 2.
6. The use of the aerobic denitrification complex bacterial agent in the improved A/O process for enhancing denitrification as claimed in claim 6, wherein the concentration of dissolved oxygen in the aerobic zone 1 is set to 2-4mg/L, and the concentration of dissolved oxygen in the aerobic zone 2 is set to 1-2 mg/L.
7. The application of the aerobic denitrification complex microbial inoculum in the improved A/O process for enhanced denitrification according to claim 1, wherein the volume ratio of the anoxic zone, the aerobic zone 1 and the aerobic zone 2 is 2:1: 1.
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