CN114557953A - 一种可自发荧光的血清白蛋白微针的制备方法 - Google Patents
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Abstract
本发明公开了一种可自发荧光的血清白蛋白微针的制备方法,包括:(1)根据植入部位所需,将牛血清白蛋白粉末溶于中性液体中,配置成白蛋白溶液,然后将戊二醛溶液与白蛋白溶液混合,得混合溶液;(2)在微针模具表面均匀涂抹三甲基氯硅烷,晾干后立即将混合溶液倒入微针模具中,然后将其置于真空泵中,抽去气泡;(3)将模具取出,置于室温下放置,待胶凝固后,将微针从微针模具中撕下,即得可自发荧光的血清白蛋白微针。本发明通过加入戊二醛促进BSA溶液交联,从而形成了稳定的透明凝胶。
Description
技术领域
本发明涉及生物材料与医药技术领域,更具体的说是涉及一种可自发荧光的血清白蛋白微针的制备方法。
背景技术
随着科学和生物医疗技术的发展,一种新型治疗方式出现了——微针给药。
1976年,Gerstel和Place提出了微针阵列用于经皮给药的概念,使用长度在5μm-100μm且具有针形状的针阵列来刺穿皮肤角质层。该针阵列采用中空针,将药物置于中空针内,并递送至角质层。随后微针的应用场景从单一的透皮给药,演变成为血糖等体液检测,深部器官手术后局部化疗,小分子、生物制剂、疫苗、细胞、DNA/RNA等透皮递送,从而实现微创,简易的检测及治疗。
但目前常规制作的透明质酸、蚕丝蛋白、明胶、血清白蛋白等生物可降解材料制作的微针无法自发荧光,而荧光是生物学中最容易应用于监测材料降解或分布的方式。而常规的可发荧光的微针需要加入荧光剂,如FITC,罗丹明等。附加的荧光剂价格高,且有生物相容性的问题。
因此,如何提供一种成本低,可自发荧光的微针属于本领域技术人员亟需解决的技术问题。
发明内容
有鉴于此,本发明提供了一种可自发荧光的血清白蛋白微针的制备方法,本发明通过增加戊二醛于血清白蛋白的微针中,改变蛋白交联方式,实现了自发荧光的功能。
为了实现上述目的,本发明采用如下技术方案:
一种可自发荧光的血清白蛋白微针的制备方法,包括以下步骤:
(1)根据植入部位所需,将牛血清白蛋白粉末溶于中性液体中,配置成白蛋白溶液,然后将戊二醛溶液与白蛋白溶液混合,得混合溶液;
(2)在微针模具表面均匀涂抹三甲基氯硅烷,晾干后立即将混合溶液倒入所述微针模具中,然后将其置于真空泵中,抽去气泡;
(3)将模具取出,置于室温下放置,待胶凝固后,将微针从所述微针模具中撕下,即得所述可自发荧光的血清白蛋白微针。
优选的,步骤(1)中所述中性液体包括水或PBS。
优选的,步骤(1)中所述白蛋白溶液质量浓度为10-20%。
上述比例制得的微针软硬适中,可刺破皮肤,且容易降解。
优选的,步骤(1)中所述戊二醛溶液质量浓度为50%。
优选的,步骤(1)中所述戊二醛溶液占所述混合溶液体积的5-15%;
更优选的,步骤(1)中所述戊二醛溶液占所述混合溶液体积的5%。
上述优选的有益效果为:戊二醛浓度越高,凝胶速度越快,5%终浓度的戊二醛溶液,成胶速度慢,易于操作。
优选的,步骤(2)中所述抽去气泡时间为4-6min。
经由上述的技术方案可知,与现有技术相比,本发明具有如下有益效果:本发明通过戊二醛促进BSA溶液交联,从而形成稳定透明的凝胶,可发红色及绿色荧光。
附图说明
为了更清楚地说明本发明实施例或现有技术中的技术方案,下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍,显而易见地,下面描述中的附图仅仅是本发明的实施例,对于本领域普通技术人员来讲,在不付出创造性劳动的前提下,还可以根据提供的附图获得其他的附图。
图1为本发明制备方法原理图;
图2为本发明实施例3所制备的微针外观形态图;
图3为本发明实施例3所制备的微针显微镜下形态图;
图4为荧光显微镜下,不同交联方式微针自发荧光的效果图。
具体实施方式
下面将结合本发明实施例中的附图,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。
实施例1
一种可自发荧光的血清白蛋白微针的制备方法,包括以下步骤:
(1)根据植入部位所需,将牛血清白蛋白粉末溶于水中,配置成质量浓度为20%的白蛋白溶液,然后将质量浓度为50%的戊二醛溶液与白蛋白溶液混合,得混合溶液;
其中,戊二醛溶液占混合溶液体积的15%;
(2)在微针模具表面均匀涂抹三甲基氯硅烷,晾干后立即将混合溶液倒入微针模具中,然后将其置于真空泵中,抽去气泡,抽6min;
(3)将模具取出,置于室温下放置,待胶凝固后,将微针从微针模具中撕下,即得可自发荧光的血清白蛋白微针。
实施例2
一种可自发荧光的血清白蛋白微针的制备方法,包括以下步骤:
(1)根据植入部位所需,将牛血清白蛋白粉末溶于水中,配置成质量浓度为10%的白蛋白溶液,然后将质量浓度为50%的戊二醛溶液与白蛋白溶液混合,得混合溶液;
其中,戊二醛溶液占混合溶液体积的5%;
(2)在微针模具表面均匀涂抹三甲基氯硅烷,晾干后立即将混合溶液倒入微针模具中,然后将其置于真空泵中,抽去气泡,抽4min;
(3)将模具取出,置于室温下放置,待胶凝固后,将微针从微针模具中撕下,即得可自发荧光的血清白蛋白微针。
实施例3
一种可自发荧光的血清白蛋白微针的制备方法,包括以下步骤:
(1)根据植入部位所需,将牛血清白蛋白粉末溶于水中,配置成质量浓度为15%的白蛋白溶液,然后将质量浓度为50%的戊二醛溶液与白蛋白溶液混合,得混合溶液;
其中,戊二醛溶液占混合溶液体积的5%;
(2)在微针模具表面均匀涂抹三甲基氯硅烷,晾干后立即将混合溶液倒入微针模具中,然后将其置于真空泵中,抽去气泡,抽5min;
(3)将模具取出,置于室温下放置,待胶凝固后,将微针从微针模具中撕下,即得可自发荧光的血清白蛋白微针。
本说明书中各个实施例采用递进的方式描述,每个实施例重点说明的都是与其他实施例的不同之处,各个实施例之间相同相似部分互相参见即可。对于实施例公开的装置而言,由于其与实施例公开的方法相对应,所以描述的比较简单,相关之处参见方法部分说明即可。
对所公开的实施例的上述说明,使本领域专业技术人员能够实现或使用本发明。对这些实施例的多种修改对本领域的专业技术人员来说将是显而易见的,本文中所定义的一般原理可以在不脱离本发明的精神或范围的情况下,在其它实施例中实现。因此,本发明将不会被限制于本文所示的这些实施例,而是要符合与本文所公开的原理和新颖特点相一致的最宽的范围。
Claims (6)
1.一种可自发荧光的血清白蛋白微针的制备方法,其特征在于,包括以下步骤:
(1)根据植入部位所需,将牛血清白蛋白粉末溶于中性液体中,配置成白蛋白溶液,然后将戊二醛溶液与白蛋白溶液混合,得混合溶液;
(2)在微针模具表面均匀涂抹三甲基氯硅烷,晾干后立即将混合溶液倒入所述微针模具中,然后将其置于真空泵中,抽去气泡;
(3)将模具取出,置于室温下放置,待胶凝固后,将微针从所述微针模具中撕下,即得所述可自发荧光的血清白蛋白微针。
2.根据权利要求1所述的一种可自发荧光的血清白蛋白微针的制备方法,其特征在于,步骤(1)中所述中性液体包括水或PBS。
3.根据权利要求1所述的一种可自发荧光的血清白蛋白微针的制备方法,其特征在于,步骤(1)中所述白蛋白溶液质量浓度为10-20%。
4.根据权利要求1所述的一种可自发荧光的血清白蛋白微针的制备方法,其特征在于,步骤(1)中所述戊二醛溶液质量浓度为50%。
5.根据权利要求1所述的一种可自发荧光的血清白蛋白微针的制备方法,其特征在于,步骤(1)中所述戊二醛溶液占所述混合溶液体积的5-15%。
6.根据权利要求1所述的一种可自发荧光的血清白蛋白微针的制备方法,其特征在于,步骤(2)中所述抽去气泡时间为4-6min。
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