CN114555618A - 发酵液中中性寡糖的分离 - Google Patents

发酵液中中性寡糖的分离 Download PDF

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Publication number
CN114555618A
CN114555618A CN202080068236.2A CN202080068236A CN114555618A CN 114555618 A CN114555618 A CN 114555618A CN 202080068236 A CN202080068236 A CN 202080068236A CN 114555618 A CN114555618 A CN 114555618A
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China
Prior art keywords
membrane
reaction environment
neutral
exchange resin
activated carbon
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Pending
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CN202080068236.2A
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English (en)
Chinese (zh)
Inventor
N·汉任
P·沙萨捏
F·马蒂森
J·托格森
M·马特维尤克
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Glycom AS
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Glycom AS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/02—Reverse osmosis; Hyperfiltration ; Nanofiltration
    • B01D61/027—Nanofiltration
    • B01D61/0271—Nanofiltration comprising multiple nanofiltration steps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/145—Ultrafiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/16—Feed pretreatment
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/58—Multistep processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/24—Rubbers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/04—Processes using organic exchangers
    • B01J39/05—Processes using organic exchangers in the strongly acidic form
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/04—Specific process operations in the feed stream; Feed pretreatment
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/08—Specific process operations in the concentrate stream
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/10—Temperature control
    • B01D2311/103—Heating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/18—Details relating to membrane separation process operations and control pH control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/26—Further operations combined with membrane separation processes
    • B01D2311/2623—Ion-Exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/26—Further operations combined with membrane separation processes
    • B01D2311/2626—Absorption or adsorption
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/26—Further operations combined with membrane separation processes
    • B01D2311/2649—Filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/26—Further operations combined with membrane separation processes
    • B01D2311/2676—Centrifugal separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/26—Further operations combined with membrane separation processes
    • B01D2311/2697—Chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2317/00—Membrane module arrangements within a plant or an apparatus
    • B01D2317/02—Elements in series
    • B01D2317/025—Permeate series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/02—Reverse osmosis; Hyperfiltration ; Nanofiltration
    • B01D61/027—Nanofiltration

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Water By Ion Exchange (AREA)
  • Water Treatment By Sorption (AREA)
CN202080068236.2A 2019-10-01 2020-10-01 发酵液中中性寡糖的分离 Pending CN114555618A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA201901154 2019-10-01
DKPA201901154 2019-10-01
PCT/IB2020/059201 WO2021064629A1 (en) 2019-10-01 2020-10-01 Separation of neutral oligosaccharides from fermentation broth

Publications (1)

Publication Number Publication Date
CN114555618A true CN114555618A (zh) 2022-05-27

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ID=75336395

Family Applications (1)

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CN202080068236.2A Pending CN114555618A (zh) 2019-10-01 2020-10-01 发酵液中中性寡糖的分离

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Country Link
US (1) US20220340942A1 (https=)
EP (1) EP4038079A4 (https=)
JP (1) JP2022550680A (https=)
KR (1) KR20220072866A (https=)
CN (1) CN114555618A (https=)
WO (1) WO2021064629A1 (https=)

Cited By (1)

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CN114994214A (zh) * 2022-06-30 2022-09-02 北京三元食品股份有限公司 一种定性检测母乳中中性低聚糖的方法

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EP4263566A4 (en) * 2020-12-18 2025-03-12 Glycom A/S Biomass removal by centrifugation
US20240349770A1 (en) 2021-04-30 2024-10-24 Dsm Ip Assets B.V. Infant formula with low level of arsenic
WO2022263425A1 (en) * 2021-06-15 2022-12-22 Dsm Ip Assets B.V. Separation of human milk oligosaccharides from a fermentation broth
CN117480000A (zh) * 2021-06-15 2024-01-30 帝斯曼知识产权资产管理有限公司 从发酵液中分离人乳寡糖
EP4355465A1 (en) * 2021-06-15 2024-04-24 DSM IP Assets B.V. Separation of human milk oligosaccharides from a fermentation broth
DK181566B1 (en) 2021-12-21 2024-05-24 Dsm Ip Assets Bv Crystallization of LNnT
CN114539433B (zh) * 2021-12-29 2022-11-01 北京三元食品股份有限公司 一种乳低聚糖的制备方法及制备的低聚糖粉、食品
DK181615B1 (en) * 2022-06-14 2024-07-02 Dsm Ip Assets Bv Separation of human milk oligosaccharides from a fermentation broth
WO2024153788A1 (en) * 2023-01-19 2024-07-25 Inbiose N.V. Purification of an oligosaccharide or oligosaccharide mixture
DK202330368A1 (en) * 2023-12-01 2025-06-24 Dsm Ip Assets Bv Separation of human milk oligosaccharides from a fermentation broth

Citations (5)

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CN105683387A (zh) * 2013-09-06 2016-06-15 格礼卡姆股份公司 寡糖的发酵生产
WO2019003133A1 (en) * 2017-06-30 2019-01-03 Glycom A/S PURIFICATION OF OLIGOSACCHARIDES
EP3456836A1 (en) * 2017-09-13 2019-03-20 Glycom A/S Separation of sialylated oligosaccharides from fermentation broth
US20190119314A1 (en) * 2016-04-19 2019-04-25 Glycom A/S Separation of oligosaccharides from fermentation broth
WO2020127140A1 (en) * 2018-12-19 2020-06-25 Basf Se Method for separating biomass from a solution comprising biomass and at least one oligosaccaride

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US6323008B1 (en) * 1997-08-14 2001-11-27 Neose Technologies, Inc. Methods for producing sialyloligosaccharides in a dairy source
US6068705A (en) * 1998-04-27 2000-05-30 Roquette Freres Process for producing low De starch hydrolysates by nanofiltration fractionation, products obtained thereby, and use of such products
DK2074221T3 (da) * 2006-10-10 2010-09-06 Wyeth Llc Forbedrede fremgangsmåder til separation af streptococcus pneumoniae type 3 polysakkarider
AU2011278236A1 (en) * 2010-07-16 2013-02-07 Glycom A/S Derivatization of oligosaccharides
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ES2991621T3 (es) * 2016-03-07 2024-12-04 Glycom As Separación de oligosacáridos de caldo de fermentación
EP3474861A4 (en) * 2016-06-24 2020-04-15 Glycom A/S COMPOUNDS COMPRISING HMOS FOR THE PREVENTION AND / OR TREATMENT OF VIRAL AND / OR BACTERIAL INFECTIONS.
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CN105683387A (zh) * 2013-09-06 2016-06-15 格礼卡姆股份公司 寡糖的发酵生产
US20190119314A1 (en) * 2016-04-19 2019-04-25 Glycom A/S Separation of oligosaccharides from fermentation broth
WO2019003133A1 (en) * 2017-06-30 2019-01-03 Glycom A/S PURIFICATION OF OLIGOSACCHARIDES
EP3456836A1 (en) * 2017-09-13 2019-03-20 Glycom A/S Separation of sialylated oligosaccharides from fermentation broth
WO2020127140A1 (en) * 2018-12-19 2020-06-25 Basf Se Method for separating biomass from a solution comprising biomass and at least one oligosaccaride

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114994214A (zh) * 2022-06-30 2022-09-02 北京三元食品股份有限公司 一种定性检测母乳中中性低聚糖的方法
CN114994214B (zh) * 2022-06-30 2023-02-24 北京三元食品股份有限公司 一种定性检测母乳中中性低聚糖的方法

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Publication number Publication date
EP4038079A1 (en) 2022-08-10
US20220340942A1 (en) 2022-10-27
WO2021064629A1 (en) 2021-04-08
JP2022550680A (ja) 2022-12-05
KR20220072866A (ko) 2022-06-02
EP4038079A4 (en) 2023-11-01

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