CN114544606A - Composition and kit for detecting content of lactic acid - Google Patents

Composition and kit for detecting content of lactic acid Download PDF

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CN114544606A
CN114544606A CN202111678921.XA CN202111678921A CN114544606A CN 114544606 A CN114544606 A CN 114544606A CN 202111678921 A CN202111678921 A CN 202111678921A CN 114544606 A CN114544606 A CN 114544606A
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lactic acid
kit
composition
reagent
sample
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蒋析文
齐文闯
吴润锋
简俊兴
雷苏炜
卢华丹
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Guangzhou Da'an Gene Co ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry

Abstract

The invention discloses a composition and a kit for detecting the content of lactic acid. The composition contains buffer solution, phenol chromogen, 4-aminoantipyrine, peroxidase, lactate oxidase, stabilizer and preservative; the kit is a liquid single-reagent type kit. The invention uses lactic acid oxidase to catalyze lactic acid to generate hydrogen peroxide, and the phenol chromogen and the 4-aminoantipyrine generate the quinone colored substance under the action of the hydrogen peroxide and the peroxidase, and the quinone colored substance has high molar absorption coefficient and high detection sensitivity. The single-reagent lactic acid detection kit provided by the invention can be used for rapidly and quantitatively determining the content of lactic acid in human serum or blood plasma, has good accuracy, precision and stability, can be stored for at least 14 days at 2-8 ℃ by opening a bottle, and meets the requirements of clinical examination.

Description

Composition and kit for detecting content of lactic acid
Technical Field
The invention relates to the technical field of medical examination, in particular to a composition and a kit for detecting the content of lactic acid.
Background
Lactic acid is an intermediate product of sugar metabolism in the body, and is mainly produced by erythrocytes, striated muscle and brain tissue, and the concentration of lactic acid in blood is mainly determined by the synthesis rate and metabolic rate of the liver and kidney. In certain pathological conditions (e.g. respiratory or circulatory failure), an increase in the blood lactate concentration can be caused by hypoxia. In addition, physiological activities such as strenuous exercise and dehydration cause an increase in the glycolysis rate during glucose metabolism in the body, which also leads to an increase in the blood lactate concentration. Lactic acid rises to a certain extent and causes lactic acidosis, which is life threatening. The severity of the underlying disease can be assessed by examining blood lactate levels.
In clinical tests, the concentration of lactic acid in blood is generally measured by a colorimetric method. The colorimetric method is to utilize lactate dehydrogenase to catalyze L-lactic acid and coenzyme to generate pyruvic acid and reduced coenzyme, the rate of the generated reduced coenzyme is in direct proportion to the concentration of lactic acid, and the content of lactic acid is calculated by measuring the generation rate of the reduced coenzyme. The reduced coenzyme has low molar absorption coefficient, so the sensitivity of the lactic acid determination by using the principle is low, and the accuracy of the determination is influenced. The other method is a lactic acid oxidase method, wherein the lactic acid is catalyzed and oxidized by using lactic acid oxidase to generate hydrogen peroxide, the chromogen and the 4-aminoantipyrine generate a quinone colored substance under the action of the hydrogen peroxide and the peroxidase, and the content of the lactic acid is calculated by measuring the absorbance of the quinone colored substance. Although the sensitivity of the lactic acid oxidase method is higher, in order to protect the stability of the chromogen, the dosage form of the reagent used by the lactic acid oxidase method in the prior art is usually double reagents, so that more instrument channels are occupied, and the detection method is more complex.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention adopts a specific composition, keeps the advantage of high sensitivity of a lactic acid oxidase method, simultaneously improves the stability and the simplicity of a reagent in the using process, reduces the occupation of an instrument channel, and provides the composition for detecting the content of the lactic acid and a kit.
An object of the present invention is to provide a composition for detecting the content of lactic acid.
Another purpose of the invention is to provide a kit for detecting the content of lactic acid.
In order to achieve the purpose, the invention is realized by the following scheme:
a composition for detecting the content of lactic acid, which comprises a buffer solution, a chromogen, 4-aminoantipyrine, peroxidase, lactate oxidase and a stabilizer; the buffer solution is a buffer solution with the pH value of 6.0-7.0; the chromogen is a phenol chromogen.
Preferably, the chromogen is one or more of 1-naphthol-3, 6-disulfonic acid sodium salt, p-chlorophenol and/or phenol.
The reason for selecting the above phenol chromogens in the present invention is that aniline chromogens (such as toss, MASO, DAOS, etc.) commonly used in the prior art are easily oxidized, and when the aniline chromogens coexist with 4-aminoantipyrine, spontaneous color development can be performed without the participation of a sample to be tested, and the color development rate is high, resulting in the color change of a reagent, so that the dosage forms of reagents used in the lactic acid oxidase method in the prior art are generally double reagents, i.e., aniline chromogens and 4-aminoantipyrine are present in different systems, respectively.
The phenol chromogens adopted by the invention, namely 1-naphthol-3, 6-disulfonic acid sodium salt, phenol and parachlorophenol, are not easy to be oxidized compared with aniline chromogens, can coexist with 4-aminoantipyrine, and can spontaneously develop color when coexisting, but the spontaneous color development rate is much slower compared with aniline chromogens and 4-aminoantipyrine, so that the two chromogens can stably coexist.
More preferably, the chromogen is 1-naphthol-3, 6-disulfonic acid sodium salt.
Preferably, the concentration of the chromogen is 0.1-10 mM.
More preferably, the concentration of the sodium salt of 1-naphthol-3, 6-disulfonate is 3 mM.
Preferably, the buffer solution is one or more of phosphate buffer solution, PIPES buffer solution and/or MES buffer solution.
More preferably, the buffer is a phosphate buffer.
More preferably, the phosphate buffer has a pH of 6.5 and a concentration of 100 mM.
Preferably, the concentration of the stabilizer is 0.5-10 g/L.
Preferably, the stabilizer is one or more of mannitol, bovine serum albumin and/or trehalose.
Preferably, the stabilizer is mannitol.
More preferably, the concentration of mannitol is 10 g/L.
Preferably, the concentration of the 4-aminoantipyrine is 2-3 mM.
Preferably, the concentration of the peroxidase is 2X 104～3×104U/L。
Preferably, the lactate oxidase is present at a concentration of 1.5X 104～2×104U/L。
Preferably, the composition further comprises a preservative, and the preservative is one or more of sodium azide, Proclin series preservatives or methylisothiazolinone.
More preferably, the preservative is sodium azide.
Preferably, the concentration of the preservative is 0.05-5 g/L.
More preferably, the concentration of the sodium azide is 1 g/L.
The components of the composition are in the same system.
The preparation method of the composition is a conventional method, namely according to the proportion of the composition, the components are respectively added into distilled water and then stirred evenly, and then are mixed, and the pH value is adjusted.
A kit for detecting the content of lactic acid is a liquid single-reagent kit and contains the composition.
The invention provides a single reagent lactic acid detection kit based on the principle of an enzyme coupling color development method, lactic acid in a sample is oxidized by lactic acid oxidase to generate hydrogen peroxide, the hydrogen peroxide generates a quinone colored substance under the action of peroxidase, 4-aminoantipyrine and phenol chromogen, and the concentration of the lactic acid in the sample is in direct proportion to the depth of the generated quinone colored substance. The content of lactic acid can be calculated by detecting the absorbance of the generated quinone colored substance, the sensitivity is high, and instrument channels are saved.
The conditions for detecting the lactic acid of the kit are as follows: the temperature is 30-37 ℃, the optical path of the cuvette is 1.0cm, the detection main wavelength is 500-546 nm, and the detection auxiliary wavelength is 700 nm.
The method for detecting the lactic acid in the sample by using the kit comprises a manual measurement method and an automatic measurement method.
The manual measuring method comprises the following steps:
s1, uniformly mixing a 2 mu L lactic acid calibrator with a 200 mu L reagent kit to obtain a standard sample; uniformly mixing 2 mu L of pure water with 200 mu L of reagent of the kit to obtain a blank sample; uniformly mixing 2 mu L of sample to be detected with 200 mu L of reagent of the kit to be used as the sample to be detected;
s2, incubating the standard sample, the blank sample and the sample to be detected for 10min at 30-37 ℃;
s3, after the incubation is finished, transferring each sample to a cuvette with an optical path of 1.0 cm. Placing the sample into a spectrophotometer, setting the detection main wavelength to be 500-546 nm and the auxiliary wavelength to be 700nm, and reading the absorbance A of a standard sampleStandard of meritAbsorbance A of blank sampleBlank spaceAnd the absorbance A of the sampleTo be measured；
S4, according to a formula:
Figure BDA0003453360090000031
and calculating to obtain the concentration of the lactic acid of the sample to be detected.
The automatic measuring method comprises the following steps:
setting parameters such as reaction time, measurement wavelength, sample amount, reagent amount and the like in a full-automatic biochemical analyzer according to the same manual measurement method, and carrying out calibration and sample measurement.
Compared with the prior art, the invention has the following beneficial effects:
the invention uses lactic acid oxidase to catalyze lactic acid to generate hydrogen peroxide, and the phenol chromogen and the 4-aminoantipyrine generate the quinone colored substance under the action of the hydrogen peroxide and the peroxidase, and the quinone colored substance has high molar absorption coefficient and high detection sensitivity. The single-reagent lactic acid determination kit provided by the invention can be used for rapidly determining the content of lactic acid in human serum or plasma, has good accuracy, precision and stability, can be stored for at least 14 days after being opened at 2-8 ℃, and meets the requirements of clinical examination.
Drawings
FIG. 1 is a graph showing the trend of the absorbance values of physiological saline measured with the kits of examples 1 to 5 and the kits of comparative examples 1 to 5 for 14 days.
FIG. 2 is a statistical chart of the linear analysis of the kit of example 1.
FIG. 3 is a statistical chart of the linear analysis of the kit of example 2.
FIG. 4 is a statistical chart of the linear analysis of the kit of example 3.
FIG. 5 is a statistical chart of the linear analysis of the kit of example 4.
FIG. 6 is a statistical chart of the linear analysis of the kit of example 5.
FIG. 7 is a statistical chart of the linear analysis of the kit of comparative example 1.
FIG. 8 is a statistical chart of the linear analysis of the kit of comparative example 2.
FIG. 9 is a statistical chart of the linear analysis of the kit of comparative example 3.
FIG. 10 is a statistical chart of the linear analysis of the kit of comparative example 4.
FIG. 11 is a statistical chart of the linear analysis of the kit of comparative example 5.
FIG. 12 is a statistical chart of the detection results of the hemoglobin interference test using the kits of examples 1 to 5.
FIG. 13 is a statistical chart of the hemoglobin interference test results of the kits of comparative examples 1 to 5.
FIG. 14 is a statistical chart of the detection results of the bilirubin interference test using the kits of examples 1-5.
FIG. 15 is a statistical chart of the detection results of the bilirubin interference test using the kits of comparative examples 1-5.
Detailed Description
The present invention will be described in further detail with reference to the drawings and specific examples, which are provided for illustration only and are not intended to limit the scope of the present invention. The test methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
Example 1 Single reagent lactic acid assay kit
1. Composition of matter
The components of the kit are shown in table 1.
TABLE 1 Single reagent lactic acid detection kit
Figure BDA0003453360090000051
2. Application method
(1) The sample can be directly used for detection without pretreatment.
(2) Manual measurement:
and uniformly mixing 2 mu L of lactic acid calibrator and 200 mu L of reagent of the kit to obtain a standard sample. 2 μ L of purified water was mixed with 200 μ L of the reagent of the kit to prepare a blank sample. And uniformly mixing 2 mu L of sample to be detected with 200 mu L of reagent of the kit to be used as the sample to be detected.
And incubating and reacting the standard sample, the blank sample and the sample to be detected at 30-37 ℃ for 10 min.
After the incubation, each sample was transferred to a cuvette with an optical path of 1.0 cm. Placing the sample into a spectrophotometer, setting the detection main wavelength to be 500-546 nm and the auxiliary wavelength to be 700nm, and reading the absorbance A of a standard sampleStandard of meritAbsorbance A of blank sampleBlank spaceAnd the absorbance A of the sampleTo be measured。
According to the formula:
Figure BDA0003453360090000052
and calculating to obtain the concentration of the lactic acid of the sample to be detected.
(3) The automatic measuring method comprises the following steps:
the calibration and sample measurement are carried out according to the same reaction time and parameters of measurement wavelength, sample amount, reagent amount and the like as those of the manual measurement method set in Hitachi 7180 of the full-automatic biochemical analyzer.
Example 2 Single reagent lactic acid assay kit
1. Composition of matter
The components of the kit are shown in table 2.
TABLE 2 Single-reagent lactic acid detection kit
Figure BDA0003453360090000061
2. Application method
The specific procedure was the same as in example 1.
Example 3 Single reagent lactic acid assay kit
1. Composition of matter
The components of the kit are shown in table 3.
TABLE 3 Single-reagent lactic acid assay kit
Figure BDA0003453360090000062
2. Application method
The specific procedure was the same as in example 1.
Example 4 Single reagent lactic acid assay kit
1. Composition of matter
The components of the kit are shown in table 4.
TABLE 4 Single-reagent lactic acid detection kit
Figure BDA0003453360090000063
Figure BDA0003453360090000071
2. Application method
The specific procedure was the same as in example 1.
Example 5 Single reagent lactic acid assay kit
1. Composition of matter
The components of the kit are shown in table 5.
TABLE 5 Single-reagent lactic acid assay kit
Figure BDA0003453360090000072
2. Application method
The specific procedure was the same as in example 1.
Comparative example 1 double-reagent lactic acid detection kit
The double-reagent lactic acid detection kit (oxidase method) is purchased from Hippocampus science and technology Co., Ltd, and the using method refers to the instruction of the kit.
Comparative example 2 single-reagent lactic acid detection kit
1. Composition of matter
The components of the kit are shown in table 6.
TABLE 6 Single-reagent lactic acid detection kit
Figure BDA0003453360090000073
2. Application method
The specific procedure was the same as in example 1.
Comparative example 3 single-reagent lactic acid detection kit
1. Composition of matter
The components of the kit are shown in table 7.
TABLE 7 Single-reagent lactic acid detection kit
Figure BDA0003453360090000081
2. Application method
The specific procedure was the same as in example 1.
Comparative example 4 single-reagent lactic acid detection kit
1. Composition of matter
The components of the kit are shown in table 8.
TABLE 8 Single-reagent lactic acid assay kit
Figure BDA0003453360090000082
2. Method of use
The specific procedure was the same as in example 1.
Comparative example 5 Single-reagent lactic acid detection kit
1. Composition of matter
The components of the kit are shown in table 9.
TABLE 9 Single-reagent lactic acid detection kit
Figure BDA0003453360090000083
Figure BDA0003453360090000091
2. Method of use
The specific procedure was the same as in example 1.
Application example 1 accuracy of Single reagent lactic acid detection kit
1. Experimental methods
(1) 20 serum samples from the hospital were collected.
(2) The kit of examples 1-5 and the kit of comparative example 1 were used to detect the lactic acid concentration of 20 serum samples, respectively, and the calibration and detection were performed on a fully automatic biochemical analyzer Hitachi 7180, and the detection results were recorded.
2. Results of the experiment
The results of measuring the lactic acid concentration in 20 samples are shown in Table 10.
TABLE 1020 lactic acid concentrations of clinical specimens
Figure BDA0003453360090000092
Based on the data shown in Table 10, regression equations were fitted to the data shown in Table 11, with the values measured with the kits of examples 1 to 5 as the X-axis and the values measured with the kit of comparative example 1 as the Y-axis.
TABLE 11 statistical results of regression analysis
Figure BDA0003453360090000101
The results in tables 10 and 11 show that the correlation between the measurement results of the kits of examples 1 to 5 and the kit of comparative example 1 is good, which indicates that the single-reagent lactic acid detection kit of the present invention has the same accuracy as the marketed double-reagent lactic acid detection kit, and meets the clinical requirements.
Application example 2 stability of Single reagent lactic acid assay kit
1. Experimental methods
(1) 20 serum samples from the hospital were collected.
(2) The kit of examples 1 to 5 and comparative examples 1 to 5 was used to perform calibration and detection on a full-automatic biochemical analyzer Hitachi 7180, the absorbance value A of physiological saline was measured every day, the detection was continued for 14 days from the day of unsealing the kit, the absorbance value A of physiological saline within 14 days was recorded, and the difference Delta A between the white absorbance on day 14 and day 1 was calculated14-1。
And storing the opened kit at 2-8 ℃.
2. Results of the experiment
The results of measuring the absorbance value A of physiological saline on day 14 are shown in Table 12.
Table 1214 blank absorbance value test results
Figure BDA0003453360090000102
According to the data in Table 12, graphs of the increase widths of the absorbance values of physiological saline measured within 14 days of unsealing of the kits of examples 1 to 5 and the kits of comparative examples 1 to 5 were constructed as shown in FIG. 1.
The results shown in table 12 and fig. 1 show that, within 14 days of unsealing, the blank absorbance values of physiological saline measured using the kits of examples 1 to 5 were small in variation width and gentle in variation trend, compared to the kits of comparative examples 2 to 5, and were not significantly different from the kit of comparative example 1 (the two-reagent lactic acid measurement kit already on the market). The reagent boxes of the examples 1 to 5 have good stability within 14 days of unsealing, wherein the reagent box of the example 1 has the smallest change width and the most gradual change trend.
Application example 3 Linear assay analysis of Single reagent lactic acid detection kit
1. Experimental methods
(1) An L-lithium lactate aqueous solution having a concentration of 16mmol/L was used as a high-value sample, and an L-lithium lactate aqueous solution was mixed with physiological saline at a certain dilution ratio with physiological saline as a zero-point sample, and diluted to 11 concentrations (0/10, 1/10, 2/10, 3/10, 4/10, 5/10, 6/10, 7/10, 8/10, 9/10, 10/10).
(2) The kit of examples 1 to 5 and the kit of comparative examples 1 to 5 were used to perform calibration and detection on a fully automatic biochemical analyzer Hitachi 7180, each concentration was measured 3 times, the test results were recorded, the average value was calculated, and a regression equation was fitted to the theoretical concentration.
2. Results of the experiment
The results of the linearity measurement are shown in Table 13.
TABLE 13 results of linear measurement of the kits of examples 1 to 5 and the kits of comparative examples 1 to 5
Figure BDA0003453360090000111
Figure BDA0003453360090000121
Linear analysis statistical plots were constructed from the data in Table 13, as shown in FIGS. 2-11.
The results in Table 13 and FIGS. 2 to 11 show that the detection of the lactic acid concentration in an aqueous solution of lithium L-lactate having a concentration of 16mmol/L using the kits of examples 1 to 5 has a better linear relationship in all of the 11 dilution gradients than the kits of comparative examples 1 to 5. The kit provided by the embodiment 1-5 of the invention has good detection linearity and meets clinical requirements.
Application example 4 anti-interference of single reagent lactic acid detection kit
1. Experimental methods
(1) Hemoglobin interference test
Dividing 6mL of serum sample into two parts (3 mL/part), adding 28 μ L500mg/dL hemoglobin water solution into one part of serum, and shaking to obtain interference serum; another part of the serum was added with 28. mu.L of purified water and shaken well to obtain a blank serum.
Mixing the interference serum and the blank serum according to a proportion to prepare a sample to be tested with the hemoglobin contents of 0mg/dL, 100mg/dL, 200mg/dL, 300mg/dL, 400mg/dL and 500 mg/dL.
The kit of examples 1 to 5 and the kit of comparative examples 1 to 5 were used to perform calibration and detection on a full-automatic biochemical analyzer Hitachi 7180, the lactic acid concentration of each sample to be measured with different hemoglobin contents was measured 2 times for each sample to be measured, and the average value and the coincidence rate of the 2 measurement results were calculated.
(2) Bilirubin interference test
Dividing 6mL of serum sample into two parts (3 mL/part) on average, adding 28 μ L of 30mg/dL bilirubin water solution into one part of serum, and shaking up to obtain interference serum; another part of the serum was added with 28. mu.L of purified water and shaken well to obtain a blank serum.
And mixing the interference serum and the blank serum according to a proportion to prepare a sample to be tested with bilirubin contents of 0mg/dL, 6mg/dL, 12mg/dL, 18mg/dL, 24mg/dL and 30 mg/dL.
The kit of examples 1 to 5 and the kit of comparative examples 1 to 5 were used to perform calibration detection on a full-automatic biochemical analyzer Hitachi 7180, the lactic acid concentration of each sample to be tested with different bilirubin contents was tested 2 times, and the average value and the concordance rate of the test results were calculated.
(3) Method for calculating consistency ratio
The mean value of the measurement of the sample at an interferent concentration of 0mg/dL was used as a blank (X)Blank space) The mean value of the measurement of the sample containing the interfering serum is used as the test control value (X)Test of) Respectively calculating the consistency rate under the condition of each concentration interferent, wherein the calculation formula is as follows:
Figure BDA0003453360090000131
2. results of the experiment
The results of the hemoglobin interference test are shown in table 14.
TABLE 14 hemoglobin interference test results
Figure BDA0003453360090000132
Figure BDA0003453360090000141
A statistical chart is constructed from the data of Table 14, as shown in FIGS. 12-13.
The results of the bilirubin interference test are shown in Table 15.
TABLE 15 bilirubin interference test results
Figure BDA0003453360090000151
A statistical chart was constructed from the data in Table 15, as shown in FIGS. 14-15.
The results in tables 14 to 15 and fig. 12 to 15 show that, compared with the kits in comparative examples 1 to 5, the consistency rates of the hemoglobin interference test and the bilirubin interference test of the kits in examples 1 to 5 of the present invention are both closer to 100%, wherein the deviation between the consistency rate of the kit in example 1 and 100% in two anti-interference tests is the smallest and the most stable. The anti-interference capability of the kit of the embodiment 1-5 on hemoglobin and bilirubin is better than that of the comparative example 1 (the kit is already on the market), and the clinical detection requirement is met.
It should be finally noted that the above examples are only intended to illustrate the technical solutions of the present invention and not to limit the scope of the present invention, and that those skilled in the art can make other variations or modifications on the basis of the above description and idea, and that all embodiments are neither necessary nor exhaustive. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims (10)

1. The composition for detecting the content of lactic acid is characterized by comprising a buffer solution, chromogen, 4-aminoantipyrine, peroxidase, lactate oxidase and a stabilizer; the buffer solution is a buffer solution with the pH value of 6.0-7.0; the chromogen is a phenol chromogen.
2. The composition of claim 1, wherein the chromogen is one or more of 1-naphthol-3, 6-disulfonic acid sodium salt, p-chlorophenol and/or phenol.
3. The composition of claim 2, wherein the chromogen is 1-naphthol-3, 6-disulfonic acid sodium salt.
4. The composition according to claim 1, wherein the buffer is one or more of phosphate buffer, PIPES buffer and/or MES buffer.
5. The composition of claim 1, wherein the buffer is a phosphate buffer.
6. The composition of claim 1, wherein the stabilizer is one or more of mannitol, bovine serum albumin and/or trehalose.
7. The composition according to claim 1, wherein the concentration of 4-aminoantipyrine is 2 to 3 mM.
8. The composition of claim 1, wherein the peroxidase is at a concentration of 2 x 104～3×104U/L。
9. The composition of claim 1, wherein the lactate oxidase is present at a concentration of 1.5 x 104～2×104U/L。
10. A kit for detecting the content of lactic acid, which is characterized in that the kit is a liquid single-reagent kit and contains the composition of any one of claims 1 to 9.
CN202111678921.XA 2021-12-31 2021-12-31 Composition and kit for detecting content of lactic acid Pending CN114544606A (en)

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