CN114525310B - Method for synthesizing basic nickel carbonate based on biomineralization reaction - Google Patents
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- 229910000008 nickel(II) carbonate Inorganic materials 0.000 title claims abstract description 41
- ZULUUIKRFGGGTL-UHFFFAOYSA-L nickel(ii) carbonate Chemical compound [Ni+2].[O-]C([O-])=O ZULUUIKRFGGGTL-UHFFFAOYSA-L 0.000 title claims abstract description 41
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 25
- 230000033558 biomineral tissue development Effects 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000002194 synthesizing effect Effects 0.000 title claims abstract description 15
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 39
- 239000004202 carbamide Substances 0.000 claims abstract description 39
- 230000001580 bacterial effect Effects 0.000 claims abstract description 23
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 claims abstract description 19
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 claims abstract description 19
- 239000011259 mixed solution Substances 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000005406 washing Methods 0.000 claims abstract description 7
- 238000012258 culturing Methods 0.000 claims abstract description 5
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 30
- 241000894006 Bacteria Species 0.000 claims description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 12
- 239000002244 precipitate Substances 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 11
- 238000011534 incubation Methods 0.000 claims description 10
- 239000008223 sterile water Substances 0.000 claims description 10
- 239000000725 suspension Substances 0.000 claims description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 241001052560 Thallis Species 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 239000012137 tryptone Substances 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 7
- 230000006698 induction Effects 0.000 claims description 6
- 241000192023 Sarcina Species 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 239000002504 physiological saline solution Substances 0.000 claims description 2
- DDBREPKUVSBGFI-UHFFFAOYSA-N phenobarbital Chemical compound C=1C=CC=CC=1C1(CC)C(=O)NC(=O)NC1=O DDBREPKUVSBGFI-UHFFFAOYSA-N 0.000 claims 1
- 238000004321 preservation Methods 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 5
- 229910052799 carbon Inorganic materials 0.000 abstract description 5
- 150000002815 nickel Chemical class 0.000 abstract description 4
- 239000006194 liquid suspension Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 description 22
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 13
- 239000002609 medium Substances 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 229910052500 inorganic mineral Inorganic materials 0.000 description 6
- 239000011707 mineral Substances 0.000 description 6
- 229910052759 nickel Inorganic materials 0.000 description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 239000013078 crystal Substances 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 238000007747 plating Methods 0.000 description 4
- 238000002411 thermogravimetry Methods 0.000 description 4
- 241001057978 Balanococcus Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229910000480 nickel oxide Inorganic materials 0.000 description 3
- GNRSAWUEBMWBQH-UHFFFAOYSA-N oxonickel Chemical compound [Ni]=O GNRSAWUEBMWBQH-UHFFFAOYSA-N 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 2
- 239000012880 LB liquid culture medium Substances 0.000 description 2
- VEQPNABPJHWNSG-UHFFFAOYSA-N Nickel(2+) Chemical compound [Ni+2] VEQPNABPJHWNSG-UHFFFAOYSA-N 0.000 description 2
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 2
- 108010046334 Urease Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000005842 biochemical reaction Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- 239000000919 ceramic Substances 0.000 description 2
- 238000002149 energy-dispersive X-ray emission spectroscopy Methods 0.000 description 2
- 239000010419 fine particle Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229910001453 nickel ion Inorganic materials 0.000 description 2
- -1 nickel orthocarbonate Chemical compound 0.000 description 2
- 229910017604 nitric acid Inorganic materials 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 229910000531 Co alloy Inorganic materials 0.000 description 1
- 238000002441 X-ray diffraction Methods 0.000 description 1
- QXZUUHYBWMWJHK-UHFFFAOYSA-N [Co].[Ni] Chemical compound [Co].[Ni] QXZUUHYBWMWJHK-UHFFFAOYSA-N 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 238000001595 flow curve Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000008176 lyophilized powder Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 150000002816 nickel compounds Chemical class 0.000 description 1
- LGQLOGILCSXPEA-UHFFFAOYSA-L nickel sulfate Chemical compound [Ni+2].[O-]S([O-])(=O)=O LGQLOGILCSXPEA-UHFFFAOYSA-L 0.000 description 1
- 229910000363 nickel(II) sulfate Inorganic materials 0.000 description 1
- KBJMLQFLOWQJNF-UHFFFAOYSA-N nickel(ii) nitrate Chemical compound [Ni+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O KBJMLQFLOWQJNF-UHFFFAOYSA-N 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
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Abstract
A method for synthesizing basic nickel carbonate based on biomineralization reaction includes such steps as culturing Bazidosporus, collecting bacterial cells, resuspending with aseptic water to obtain the liquid suspension, adding it to the mixed solution of urea and nickel chloride, biomineralization reaction at room temp, collecting deposit, washing and drying. The design has the advantages of good reaction stability, mild condition, simple steps, no harmful pollution, higher carbon-containing ratio and suitability for the intermediate of the preparation of the organic nickel salt.
Description
Technical Field
The invention belongs to the field of inorganic material synthesis, and particularly relates to a method for synthesizing basic nickel carbonate based on a biomineralization reaction.
Background
Nickel carbonate is an important nickel compound, and the currently known forms are nickel orthocarbonate, basic nickel carbonate and acid nickel carbonate. As an important inorganic fine chemical, basic nickel carbonate is widely used in the fields of industrial catalysts, precision plating, printed circuit board plating, general alloy plating, nickel-cobalt alloy plating, ceramic industry and the like. Basic nickel carbonate is a raw material for preparing a plurality of nickel salts and is an emerging chemical product which gradually replaces the traditional petrochemical catalyst.
There are various industrial methods for synthesizing basic nickel carbonate, and the molecular formula is xNiCO 3·yNi(OH)2·zH2 O because of the different preparation methods. The most common method is a sodium carbonate method, which is to add nitric acid into sulfuric acid to prepare mixed acid, then add metallic nickel to react with nitric acid to generate nickel nitrate, then react with sulfuric acid to generate nickel sulfate solution, then add sodium carbonate to react to generate basic nickel carbonate, and then filter, concentrate, cool and crystallize, centrifugally separate to prepare the basic nickel carbonate finished product. The existing synthesis method has the problems of high temperature and time requirements, complex post-treatment steps, high cost and difficult removal of harmful impurities.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provide a method for synthesizing basic nickel carbonate based on biomineralization reaction, which has mild synthesis conditions, simple steps and no harmful pollution.
In order to achieve the above object, the present invention provides the following technical solutions:
a method for synthesizing basic nickel carbonate based on biomineralization reaction sequentially comprises the following steps:
firstly, culturing sarcina bardana, collecting thalli and re-suspending the thalli by using sterile water to obtain re-suspension bacteria liquid;
and secondly, adding the resuspension bacterial solution into a mixed solution of urea and nickel chloride, performing biological induction mineralization reaction at room temperature, collecting precipitate, and sequentially washing and drying to obtain basic nickel carbonate.
In the second step, the concentration of urea and nickel chloride in the mixed solution of urea and nickel chloride is 0.4-0.6mol/L and 0.008-0.012mol/L respectively, and the volume ratio of the resuspension bacterial liquid to the mixed solution of urea and nickel chloride is 1:0.8-1.2.
In the first step, the OD value of the re-suspension bacteria liquid is 2.5-4.0.
In the second step, the pH value of the system is regulated to 7.5-8.5 after the resuspension bacterial liquid is added into the mixed solution of urea and nickel chloride, so that the biological induction mineralization reaction is carried out in an alkaline environment.
In the first step, the Balanococcus barbiturae has a deposit number of ATCC11859, and the culture method comprises the following steps:
under the aseptic environment, the bacillus octazier is inoculated into LB solid medium added with urea for culture, and then single colony is picked and inoculated into LB liquid medium added with urea for incubation.
The preparation method of the re-suspension bacterial liquid comprises the following steps:
and centrifuging the bacterial liquid after incubation, taking a precipitate, washing the precipitate with sterile physiological saline and sterile water in sequence, and finally adding sterile water for resuspension to obtain a resuspension bacterial liquid with the same bacterial liquid volume as that after incubation.
The formula of the LB solid medium added with urea comprises the following components: 9-11g/L of sodium chloride, 9-11g/L of tryptone, 4.5-5.5g/L of yeast powder, 9-11g/L of urea and 18-22g/L of agar;
The formula of the LB liquid medium added with urea comprises the following components: 9-11g/L of sodium chloride, 9-11g/L of tryptone, 4.5-5.5g/L of yeast powder and 9-11g/L of urea.
In the second step, the time of the biological induction mineralization reaction is 10-30min.
Compared with the prior art, the invention has the beneficial effects that:
According to the method for synthesizing basic nickel carbonate based on biomineralization reaction, disclosed by the invention, the bacillus octastack is cultured firstly, then thalli are collected and resuspended by using sterile water to obtain a resuspension bacterial solution, then the resuspension bacterial solution is added into a mixed solution of urea and nickel chloride, the biomineralization reaction is carried out at room temperature, finally the collected precipitate is sequentially washed and dried, so that the basic nickel carbonate is obtained. Therefore, the method has the advantages of good reaction stability, mild conditions, simple steps, no harmful pollution, higher carbon-containing ratio and suitability for the intermediate for preparing the organic nickel salt.
Drawings
Fig. 1 is an SEM test chart of the product obtained in example 1.
Fig. 2 is an SEM test chart of the product obtained in example 3.
Fig. 3 is an SEM test chart of AR-grade chemically synthesized basic nickel carbonate.
Fig. 4 is an SEM test chart of the product obtained in the comparative example.
FIG. 5 shows microscopic observations of the products obtained in example 1 and example 3 and AR grade chemically synthesized basic nickel carbonate.
FIG. 6 is a thermogravimetric TGA analysis of the product obtained in example 1.
FIG. 7 is a thermogravimetric TGA analysis of the product obtained in example 3.
In fig. 6 and 7, a curve 1 is a mass change curve, a curve 2 is a heat flow curve, the curve is convex to represent heat release, a curve 3 is a temperature change curve, and data in a box are data of curves 1, 2 and 3 at time 0, namely initial data.
Detailed Description
The invention is further described below with reference to the detailed description and the accompanying drawings.
The invention provides a method for synthesizing basic nickel carbonate based on biomineralization reaction, which utilizes Balanococcus baryophyllus (purchased from Beijing biological collection center, no. ATCC 11859) to secrete urease to the outside, can decompose the Balanococcus baryophyllus in urea-containing environment and perform a series of biochemical reactions to obtain carbonate ions and raise the pH of the outside, meanwhile, the surface of the bacteria contains a plurality of protein, sugar, lipid and other components, a plurality of groups exist in the components, divalent nickel ions can be adsorbed on the surface of bacteria by means of electrostatic adsorption of the groups, and the divalent nickel ions and the extracellular carbonate ions and hydroxyl ions are combined to generate basic nickel carbonate, and biological components on the surface of the bacteria and the extracellular urease can regulate the generation amount and speed of mineralizes, so that nickel ores with special sizes and shapes are formed on the surface.
Compared with a chemical method, the mineral synthesized by the biomineralization reaction has large specific surface area and better stability. Meanwhile, the Bazidosporium barbitum is an environment-friendly bacterium, and a plurality of polypeptides are attached to the surface of minerals in the whole biochemical reaction process, so that the hydrophilicity of the Bazidosporium barbitum is improved, and the Bazidosporium barbitum has the conformational advantage of natural products.
Example 1:
A method for synthesizing basic nickel carbonate based on biomineralization reaction is carried out sequentially according to the following steps:
1. Under a sterile environment, the bacillus thuringiensis sarcina is inoculated into an LB solid culture medium added with urea through a plate streaking mode, a plate colony is obtained by culturing for 36 hours at 37 ℃, then a single colony is selected and inoculated into 3ml of LB liquid culture medium added with urea, the culture medium is subjected to shaking table incubation for 24 hours at 30 ℃, then bacterial liquid is inoculated into the LB liquid culture medium according to the volume ratio of 1:100, and the culture medium is subjected to shaking table incubation for 24 hours again at 30 ℃, wherein the formula of the LB solid culture medium added with urea is as follows: 10g/L of sodium chloride, 10g/L of tryptone, 5g/L of yeast powder, 10g/L of urea and 20g/L of agar, wherein the formula of the LB liquid medium added with urea is as follows: 10g/L of sodium chloride, 10g/L of tryptone, 5g/L of yeast powder and 10g/L of urea;
2. Centrifuging the bacterial liquid after incubation at 4 ℃ and taking a precipitate, washing the precipitate with sterile normal saline once and sterile water twice in sequence, and then adding sterile water to resuspend to obtain a resuspension bacterial liquid with the same bacterial liquid volume and OD value of 3.5 as the bacterial liquid after incubation;
3. Mixing the resuspension bacterial liquid with a mixed solution of urea and nickel chloride in equal volume at room temperature, quickly turning the solution into a light green suspension, standing for 10min, settling the suspension, centrifuging at the temperature of 4 ℃ at the time, washing the sediment twice with sterile water, and vacuum freeze-drying the washed sediment at the temperature of-60 ℃ to obtain green basic nickel carbonate freeze-dried powder, wherein the concentration of urea and nickel chloride in the mixed solution of urea and nickel chloride is 0.5mol/L and 0.01mol/L respectively.
If removal of the bacterial cells from the precipitate is desired, it may be separated by passing it through a 0.45 μm filter.
Example 2:
The difference from example 1 is that:
In the step 1, the formula of the LB solid medium added with urea is as follows: 9g/L of sodium chloride, 9g/L of tryptone, 4.5g/L of yeast powder, 9g/L of urea and 18g/L of agar, wherein the formula of the LB liquid medium added with urea is as follows: 9g/L of sodium chloride, 9g/L of tryptone, 4.5g/L of yeast powder and 9g/L of urea;
in the step 2, the OD value of the re-suspension bacteria liquid is 3.2;
in the step 3, the volume ratio of the re-suspension bacteria liquid to the mixed solution of urea and nickel chloride is 1:1.2, and the standing time is 20min.
Example 3:
The difference from example 1 is that:
In the step 3, the resuspended bacteria liquid is mixed with the mixed solution of urea and nickel chloride in equal volume at room temperature, and then sodium hydroxide solution is added dropwise, the pH value of the system is regulated to 8.0, the biological induction mineralization reaction is carried out in alkaline environment, the obtained precipitate after centrifugation is washed and then is dried in a hot blast vacuum drying oven at 80 ℃, and the obtained precipitate is ground into fine particles by a mortar, so that the product is green.
Comparative example:
The procedure was as in example 1, except that:
in the step 3, the resuspension bacteria liquid and the nickel chloride solution are mixed in equal volume at room temperature and then are kept stand for 30min, and the concentration of nickel chloride in the nickel chloride solution is 0.01mol/L.
To investigate the chemical nature of the product according to the invention, the following tests were carried out:
1. SEM test
SEM tests were performed on the products obtained in example 1, example 3 and comparative example and AR-grade chemically synthesized basic nickel carbonate at the same time, and the results are shown in fig. 1 to 4.
As can be seen from FIGS. 1 to 4, in the basic nickel carbonate lyophilized powder obtained in example 1, spherical nickel minerals are formed on the surface and outside of bacteria, the diameter is 50-100nm, the bacteria are complete in shape, the surface minerals are few, and no caking exists. In the fine particles obtained in example 3, nickel minerals and thalli were mixed together, and had irregular morphology, unequal size, relatively large volume, broken thalli morphology and small amount of caking. In the freeze-dried powder obtained in the comparative example, no nickel mineral is generated on the surface of the bacteria, and the shape of the freeze-dried powder is the same as that of bacterial thalli, and the freeze-dried powder is in a rod shape. The crystal diameter of AR-grade chemically synthesized basic nickel carbonate is 200-600nm, and the basic nickel carbonate is nearly spherical and relatively aggregated.
2. Microscopic observation
The results of observing the particulate state of the products obtained in example 1 and example 3 and the AR-grade chemically synthesized basic nickel carbonate under an optical microscope (1000 times) are shown in fig. 5.
As can be seen from fig. 5, the product obtained in example 1 and AR-grade chemically synthesized basic nickel carbonate particles were finer and more dispersed than example 3.
3. Crystal investigation
The products obtained in example 1, example 3 and comparative example and AR grade chemically synthesized basic nickel carbonate were subjected to EDX and XRD analyses simultaneously, and the results are shown in Table 1:
TABLE 1 EDX and XRD analysis results for four materials
As is clear from Table 1, the products obtained in example 1 and example 3 all belong to basic nickel carbonate, but the crystal forms are different from each other, and the product obtained in example 3 has the same crystal form as the chemically synthesized basic nickel carbonate. The products obtained in examples 1 and 3 have a higher carbon content than the chemically synthesized basic nickel carbonate, as seen in the relative mass fractions of carbon and nickel elements in the respective materials, whereas the products obtained in comparative examples have no nickel element and only the carbon element of the bacteria themselves.
4. Thermogravimetric TGA analysis
Thermal gravimetric TGA analysis was performed on the products obtained in example 1 and example 3, respectively, and the results are shown in fig. 6 and 7.
As can be seen from FIG. 6, the product obtained in example 1, with increasing temperature, has a weight which increases and decreases, and finally tends to stabilize, and the overall weight changes little, and the temperature increases about 300 ℃ and decreases rapidly, thus giving off a large amount of heat to be converted into nickel oxide, which accords with the characteristics of basic nickel carbonate, and can be used as a ceramic colorant.
As can be seen from FIG. 7, the product obtained in example 3 has a weight that increases with temperature and decreases after increasing, and finally tends to be stable, the overall weight is not greatly changed, the temperature increases about 300 ℃ and decreases rapidly, a large amount of heat is released, and the product is converted into nickel oxide, accords with the characteristics of basic nickel carbonate, and can be used for preparing nickel oxide or organic nickel salt.
Claims (5)
1. A method for synthesizing basic nickel carbonate based on biomineralization reaction is characterized in that:
The method sequentially comprises the following steps:
firstly, culturing the spore sarcina bardana, collecting thalli and re-suspending the thalli by using sterile water to obtain a re-suspension bacterial liquid, wherein the preservation number of the spore sarcina bardana is ATCC11859;
adding the resuspension bacteria solution into a mixed solution of urea and nickel chloride, regulating the pH of the system to 7.5-8.5, performing biological induction mineralization reaction at room temperature in an alkaline environment, collecting precipitate, sequentially washing and drying to obtain basic nickel carbonate, wherein the concentration of the urea and the nickel chloride in the mixed solution of urea and nickel chloride is 0.4-0.6mol/L and 0.008-0.012mol/L respectively, the volume ratio of the resuspension bacteria solution to the mixed solution of urea and nickel chloride is 1:0.8-1.2, and the time of the biological induction mineralization reaction is 10-30min.
2. The method for synthesizing basic nickel carbonate based on biomineralization reaction according to claim 1, wherein: in the first step, the OD value of the re-suspension bacteria liquid is 2.5-4.0.
3. The method for synthesizing basic nickel carbonate based on biomineralization reaction according to claim 1, wherein:
in the first step, the culturing method of the sarcina barbita comprises the following steps:
under the aseptic environment, the bacillus octazier is inoculated into LB solid medium added with urea for culture, and then single colony is picked and inoculated into LB liquid medium added with urea for incubation.
4. A method for synthesizing basic nickel carbonate based on biomineralization reaction according to claim 3, wherein:
The preparation method of the re-suspension bacterial liquid comprises the following steps:
and centrifuging the bacterial liquid after incubation, taking a precipitate, washing the precipitate with sterile physiological saline and sterile water in sequence, and finally adding sterile water for resuspension to obtain a resuspension bacterial liquid with the same bacterial liquid volume as that after incubation.
5. A method for synthesizing basic nickel carbonate based on biomineralization reaction according to claim 3, wherein:
the formula of the LB solid medium added with urea comprises the following components: 9-11g/L of sodium chloride, 9-11g/L of tryptone, 4.5-5.5g/L of yeast powder, 9-11g/L of urea and 18-22g/L of agar;
The formula of the LB liquid medium added with urea comprises the following components: 9-11g/L of sodium chloride, 9-11g/L of tryptone, 4.5-5.5g/L of yeast powder and 9-11g/L of urea.
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