CN103289919A - Urease-producing microorganisms and method for solidifying heavy metals in subgrade by using same - Google Patents
Urease-producing microorganisms and method for solidifying heavy metals in subgrade by using same Download PDFInfo
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Abstract
The provided urease-producing microorganisms comprise: Sporosarcina antarctica UR53, Sporosarcina koreensis UR47, Sporosarcina sp. UR31, and Bacillus lentus UR41, wherein all the above microorganisms have been deposited with the China General Microbiological Culture Collection Center (CGMCC) on March 16, 2012, and assigned respectively the accession numbers: CGMCC NO.5916, CGMCC NO.5915, CGMCC NO.5914, and CGMCC NO.5913. The provided method for solidifying heavy metals in subgrade using the urease-producing microorganisms comprises steps of: putting the above four microorganisms, Sporosarcina pasteurii and Terrabacter tumescens in a fermentation medium to obtain a bacterial solution by fermenting; adding a urea reaction solution into the bacterial solution; and adding the solution of the above step into a solution containing heavy metals to form a mixed solution, so that floccules of microorganism-heavy-metal are formed in the mixed solution and water-insoluble heavy-metal-carbonates are formed further. The method for solidifying heavy metals in subgrade using the microorganisms provided by the invention has the advantages of short solidifying time, good effect, low cost, and no secondary pollution to environment.
Description
Technical field
The invention belongs to microorganism and curing heavy metal technical field thereof, the method that is specifically related to the urease-producing microorganism and solidifies heavy metal in the ground.
Background technology
Heavy metal contamination refers to because Human's production and activity, causes that heavy metal content because heavy metal ion has long-term delay and nondegradable characteristic, has caused very big destruction to ecotope apparently higher than its background value in the environment.Along with large-scale urban sprawl and construction, many buildings construction are near discarded factory and refuse tip, and heavy metal contamination is serious in the ground.Heavy metal element can finally enter in the organism by food chain, destroys the organism normal metabolic, and the serious harm HUMAN HEALTH has become very important environmental problem.
Traditional heavy metal treatment process mainly comprises: chemical precipitation method, ion exchange method, evaporation concentration method, electrolytic process, gac and silica gel adsorption and membrane separation process etc., removal is thorough, expense is expensive, produce shortcomings such as poisonous mud or other waste materials but these methods exist.Therefore, heavy metal treatment technology and the technology of research and development efficient and environment-friendly type become one of focus of research.
The development of modern biotechnology comes into one's own microbial treatment heavy metal contamination gradually.Microorganism treatment is to utilize biomaterial and the removal of life Metabolic activity and/or accumulation heavy metals such as bacterium, fungi, algae, thereby reduces the concentration of heavy metal ion in the edatope.Compare with traditional treatment technology and to have clear superiority, low as its processing cost, treatment effect is good, and biochemical treatment after stain thing residual quantity can reach very low-level, thereby this technology becomes the recovery technique that development potentiality and market outlook are arranged most.
Summary of the invention
In order to solve the problem that above-mentioned prior art exists, the method that the object of the present invention is to provide the urease-producing microorganism and solidify heavy metal in the ground, adopt microorganism of the present invention to solidify heavy metal in the ground, have set time short, effective, cost is low, and can not cause secondary pollution to environment.
In order to achieve the above object, the technical solution adopted in the present invention is:
Urease-producing microorganism: circle spore gemma sarcina Sporosarcina antarctica UR53, Korea S gemma sarcina Sporosarcina koreensis UR47, gemma sarcina Sporosarcina sp.UR31 and bacillus lentus Bacillus lentus UR41 have been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on March 16th, 2012, deposit number is respectively CGMCC No.5916, CGMCC No.5915, CGMCC No.5913, CGMCC No.5914, CGMCC is called for short at this preservation center, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101.
It is oval shaft-like that described round spore gemma sarcina Sporosarcina antarctica UR53, Korea S gemma sarcina Sporosarcina koreensis UR47, gemma sarcina Sporosarcina sp.UR31 and bacillus lentus Bacillus lentus UR41 all are, gemma is arranged, no pod membrane, Gram-positive.At NH
4-YE is yeast extract 20g/L, and on the ammonium sulfate 10g/L flat board, bacterium colony is rounded, and surface wettability is smooth, neat in edge, and the bacterium colony size is 1-2mm, and it is faint yellow that bacterium colony is, and this bacterium all can grow under the scope of 4 ℃-37 ℃ substratum temperature and pH7-9.5.
The urease-producing microorganism is solidified the method for heavy metal in the ground, comprises the steps:
Step 1: microorganism Sporosarcina pasteurii, Terrabacter tumescens, circle spore gemma sarcina Sporosarcina antarctica UR53, Korea S gemma sarcina Sporosarcina koreensis UR47, gemma sarcina Sporosarcina sp.UR31 and the single bacterium colony of bacillus lentus Bacillus lentus UR41 were obtained six kinds of bacterium liquid respectively in fermentation culture 12-60 hour in fermention medium under 25 ℃ of-37 ℃ of conditions;
Step 2: add the reaction solution urea soln that concentration is 0.01-2mol/L respectively in six kinds of bacterium liquid that obtain to step 1, it is 1:1-1:20 that bacteria liquid amasss with the urea soln volume ratio, the bacterium liquid that will add the reaction solution urea soln again joins respectively to contain in the heavy metal solution that heavy metal concentration is 0.1g/L-5g/L and forms mixing solutions, heavy metal solution is long-pending than being 1:1-1:100 with the bacteria liquid that contains the reaction solution urea soln, make to form microorganism-heavy metal flocs unit in the mixing solutions, and then generate water-fast heavy metal carbonate.
The described microorganism Sporosarcina of step 1 pasteurii comes from US mode culture collection warehousing (American type culture collection), is numbered ATCC11859; Described microorganism Terrabacter tumescens comes from China Committee for Culture Collection of Microorganisms common micro-organisms center, is numbered AS.1.2690
The described fermention medium of step 1 comprises yeast extract 10-20g/L, and ammonium sulfate or ammonium chloride 10g/L, pH are 7-9.5.
The present invention compares with prior art, has following advantage:
1, screen the microorganism of energy curing heavy metal from existing urease-producing bacterial strain, soil sample and water sample, and unknown bacterium is carried out Molecular Identification, the microorganism strains that can effectively administer heavy metal contamination for acquisition provides the candidate resource;
2, urea is under the effect of the urase that Institute of Micro-biology produces, be decomposed into ammonium root and carbonic acid gas, make bacterium pH rising on every side simultaneously, impel and form microorganism-heavy metal flocs unit in the solution, further generate water-fast heavy metal carbonate, thereby heavy metal ion is cured; Have heavy metal contamination and solidify the time weak point of administering, effective, the heavy metal ion curing degree is reached more than 90%;
3, the cost of used nutritive medium is low;
4, among the present invention the microorganism that utilizes be microorganism itself that exist in the ground (soil) or the culture of certain microorganism wherein, nutritive substance also is crude substance, can not cause secondary pollution to environment.
Description of drawings
Fig. 1 is the urease-producing bacterial strain phylogenetic tree according to 16S rDNA sequence construct.
Fig. 2 (uses OD for the biomass of cultivating bacterial strain
600Value representation) and urease activity, X-coordinate is different types of urease-producing bacterial strain, and ordinate zou is biomass and urease activity.
Fig. 3 is for removing rate to the precipitation of different sorts heavy metal, and X-coordinate is the urease-producing bacterial strain of different kind, and the curing of ordinate zou heavy metal removes rate.
Fig. 4 is the heavy metal precipitation curve, and X-coordinate is the time, and hour to be unit, the curing of ordinate zou heavy metal removes rate.
Fig. 5 is the stereoscan photograph of the cured article of heavy metal; Wherein
Fig. 5 a is the stereoscan photograph of the cured article of heavy metal nickel;
Fig. 5 b is the stereoscan photograph of the cured article of heavy metal copper;
Fig. 5 c is the stereoscan photograph of the cured article of heavy metal lead;
The attach most importance to stereoscan photograph of cured article of cobalt metal of Fig. 5 d;
Fig. 5 e is the stereoscan photograph of the cured article of heavy metal zinc;
Fig. 5 f is the stereoscan photograph of the cured article of heavy metal cadmium.
Embodiment
Below in conjunction with the drawings and specific embodiments the present invention is described in further detail.
Embodiment:
Urease-producing has the separation screening of curing heavy metal ability bacterial strain
1, sample collecting
Sporosarcina pasteurii comes from US mode culture collection warehousing (American type culture collection), be numbered ATCC11859, Terrabacter tumescens comes from China Committee for Culture Collection of Microorganisms common micro-organisms center, is numbered AS.1.2690.
2, the separation of urease-producing bacterial strain and screening
The urease-producing strains separation is gathered pedotheque from the Tsing-Hua University flower nursery.
Bacterium has urea decomposing enzyme, can produce a large amount of ammonia by decomposing urea, makes substratum be alkalescence, shows red.This experiment utilizes this characteristic, test sample is first at 37 ℃, under the 5M high concentration urea condition behind the enrichment culture 24h, kill the various microbial nutrition somatocyte that can not tolerate and utilize high concentration urea, nutrient solution after will handling again carries out gradient dilution, coating urase screening and culturing flat board, cultivate down for 37 ℃, the bacterial strain that picking reddens the substratum color, line separates single bacterium colony, the microorganism that obtains is the urease-producing microorganism, and utilize 16S rDNA method to identify, difference called after circle spore gemma sarcina Sporosarcina antarctica UR53, Korea S gemma sarcina Sporosarcina koreensis UR47, gemma sarcina Sporosarcina sp.UR31, bacillus lentus Bacillus lentus UR41; Fig. 1 is the urease-producing bacterial strain phylogenetic tree according to 16S rDNA sequence construct.
The substratum heavy metals immobilization of urease-producing bacterial strain
1, strain culturing
Preparation fermention medium: yeast extract 10-20g/L, ammonium sulfate or ammonium chloride 10g/L, pH is 7-9.5, the 100ml fermention medium packed into sterilize in the 500ml culturing bottle, the single bacterium colony Sporosarcina of picking pasteurii from flat board respectively, Terrabacter tumescens, circle spore gemma sarcina Sporosarcina antarctica UR53, Korea S gemma sarcina Sporosarcina koreensis UR47, gemma sarcina Sporosarcina sp.UR31 and bacillus lentus Bacillus lentus UR41 are inoculated in the fermention medium respectively, cultivate under 30 ℃ of temperature, rotating speed is 150rpm-250rpm.Cultivate and collect bacterium liquid after 16 hours, detect the biomass of six kinds of bacterium liquid and (use OD
600Value representation) and urease activity, detected result as shown in Figure 2.
2, the curing of heavy metal
Get six kinds of bacterium liquid 1ml respectively, the urea soln that adds equal-volume 0.5mol/L is made mixing solutions, and every kind of bacterium liquid prepares six kinds of parallel samples, with volume be again the mixing solutions of 2ml to join volume respectively be 0.5ml, concentration is the heavy metal solution NiCl of 2g/L
2, CuCl
2, PbCl
2, CoCl
2, ZnCl
2And CdCl
2In, the result shows, all urease-producing bacterial strains to the curing clearance of above six heavy metal species all more than 88%, the curing clearance of the copper of UR47 and lead is the highest, the curing clearance of the cobalt of UR31 and zinc is the highest, the curing clearance of the nickel of Terrabacter tumescens and cadmium is the highest, and the result as shown in Figure 3.
In experiment, also get bacterium liquid respectively: Korea S gemma sarcina Sporosarcina koreensis UR47, gemma sarcina Sporosarcina sp.UR31, Terrabacter tumescens, the urea soln that adds different concns respectively, bacteria liquid is long-pending to be respectively 1:1 with the urea soln volume ratio, 1:10,1:20, making the urea final concentration is 0.25mol/L, get two samples of the Korea S gemma sarcina Sporosarcina koreensis UR47 solution that contains urea soln, add the copper solutions that concentration is 0.5g/L respectively, concentration is the lead solution of 5g/L, and two samples are 1:10 and 1:100 with the volume ratio of copper solutions and lead solution respectively; Get two samples of the gemma sarcina Sporosarcina sp.UR31 solution that contains urea soln, add cobalt and zinc solution respectively, get two samples of the Terrabacter tumescens solution that contains urea soln, add nickel and cadmium solution respectively, the concentration of metallic solution adds identical with volume ratio with Korea S gemma sarcina Sporosarcina koreensis UR47 and metallic solution.The result shows that under different bacterium liquid, urea, concentration of heavy metal ion condition, the deposition of heavy metal ion is all more than 88%.
3, heavy metals immobilization speed
Analyze the Korea S gemma sarcina Sporosarcina copper of koreensis UR47 and lead respectively, the cobalt of gemma sarcina Sporosarcina sp.UR31 and zinc, the curing clearance of the nickel of Terrabacter tumescens and cadmium over time, the result as shown in Figure 4, the solidification process of this six heavy metal species all mainly occurred in preceding 20 minutes that add bacterium liquid, reached maximum value at 48 hours.
Analysis to formation heavy metals immobilization thing
1, the analysis of heavy metals immobilization thing
Sedimentable matter is carried out XRD analysis, and formed heavy metals immobilization thing is heavy metal carbonate.Throw out is carried out electron microscopic observation, the result as shown in Figure 5, Fig. 5 a wherein, Fig. 5 d is heavy metal Ni and Co cured article, is the hexahedron shape, length of side 10-40 μ m; Fig. 5 b, Fig. 5 f is the cured article of heavy metal Cu and Cd, is spherical, diameter 5-10 μ m; Fig. 5 c and Fig. 5 e are the cured articles of heavy metal Pb and Zn, are needle-like length at 20-50 μ m.
2, the acidproof property analysis of heavy metals immobilization thing
The formed heavy metals immobilization thing of microorganism is exposed in the air, can be subjected to the threat of acid rain in the environment, therefore, needs to detect its acidproof character.With the acidproof character that the sulfuric acid of a series of pH values is tested the heavy metals immobilization thing that forms, the pH value is respectively: 0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0,4.5,5.0, and5.5.Whether begin test from the sulfuric acid of pH5.5, drip sulfuric acid to the sand post of bonding, examine two minutes with magnifying glass, observing has bubble to generate.If there is not bubble to generate, then can tolerate the acid of this pH, the sulfuric acid with next pH value continues experiment then, and up to there being bubble to generate, then acidproof ability is the pH value that produces the previous sulfuric acid of bubble.The tolerance acid pH value that the results are shown in Table 1, six heavy metal species cured article is 2.0, can be in acid rain environment stable existence.
The acidproof experimental result of table 1 heavy metals immobilization thing (+: no bubble generation-: have bubble to produce)
Claims (4)
1. urease-producing microorganism, it is characterized in that: described urease-producing microorganism is bacillus lentus Bacillus lentus UR41, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on March 16th, 2012, deposit number is CGMCC No.5914, CGMCC is called for short at this preservation center, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101.
2. urease-producing microorganism according to claim 1 is characterized in that: described bacillus lentus Bacillus lentus UR41 is oval shaft-like, gemma is arranged, no pod membrane, Gram-positive; At NH
4-YE is yeast extract 20g/L, and on the ammonium sulfate 10g/L flat board, bacterium colony is rounded, and surface wettability is smooth, neat in edge, and the bacterium colony size is 1-2mm, and it is faint yellow that bacterium colony is, and this bacterium all can grow under the scope that 4 ℃-37 ℃ substratum temperature and pH are 7-9.5.
3. the method for heavy metal in the described urease-producing microorganism curing of claim 1 ground is characterized in that: comprise the steps:
Step 1: the single bacterium colony of described bacillus lentus Bacillus lentus UR41 was obtained bacterium liquid in fermentation culture 12-60 hour in fermention medium under 25 ℃ of-37 ℃ of conditions;
Step 2: adding concentration in the bacterium liquid that obtains to step 1 is the reaction solution urea soln of 0.01-2mol/L, it is 1:1-1:20 that bacteria liquid amasss with the urea soln volume ratio, the bacterium liquid that will add the reaction solution urea soln again joins to contain in the heavy metal solution that heavy metal concentration is 0.1g/L-5g/L and forms mixing solutions, heavy metal solution is long-pending than being 1:1-1:100 with the bacteria liquid that contains the reaction solution urea soln, make to form microorganism-heavy metal flocs unit in the mixing solutions, and then generate water-fast heavy metal carbonate.
4. method according to claim 3, it is characterized in that: the described fermention medium of step 1 comprises yeast extract 10-20g/L, ammonium sulfate or ammonium chloride 10g/L, pH are 7-9.5.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN107828838A (en) * | 2017-12-06 | 2018-03-23 | 东莞理工学院 | South Korea gemma sarcine CGMCC No.5915 are applied and lipopeptid class Surfactin preparation method and composition |
| JP2018527181A (en) * | 2015-08-27 | 2018-09-20 | バイオセメント テクノロジーズ， インコーポレイテッド | Bioremediation of geological materials contaminated with heavy metals by resident microorganisms |
| CN108675585A (en) * | 2018-06-19 | 2018-10-19 | 浙江工业大学 | Method for solidifying and cementing heavy metal-containing tannery sludge by using bacillus pasteurii |
| CN109047269A (en) * | 2018-06-19 | 2018-12-21 | 浙江工业大学 | Method for solidifying and cementing heavy metal cobalt-containing industrial waste residue by using staphylococcus cohnii ureolytic subspecies |
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| CN114107090A (en) * | 2021-10-26 | 2022-03-01 | 广东以色列理工学院 | Biochemical composite material and preparation method and application thereof |
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| CN115975862A (en) * | 2022-10-26 | 2023-04-18 | 安徽农业大学 | Sporosarcina korea JZ-2 and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007069884A1 (en) * | 2005-12-15 | 2007-06-21 | Stichting Deltares | Immobilisation of bacteria to a geological material |
-
2012
- 2012-04-23 CN CN201310145479.3A patent/CN103289919B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007069884A1 (en) * | 2005-12-15 | 2007-06-21 | Stichting Deltares | Immobilisation of bacteria to a geological material |
Non-Patent Citations (3)
| Title |
|---|
| 周志峰 等: "1株芽抱杆菌吸附镉和铜的研究", 《西南农业大学学报（自然科学版）》, vol. 28, no. 3, 30 June 2006 (2006-06-30) * |
| 翁添富 等: "土壤重金属污染生物修复技术研究进展", 《科技导报》, vol. 27, no. 4, 31 December 2009 (2009-12-31) * |
| 陈勇生 等: "重金属的生物吸附技术研究", 《环境科学进展》, vol. 5, no. 6, 31 December 1997 (1997-12-31) * |
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| CN109047269A (en) * | 2018-06-19 | 2018-12-21 | 浙江工业大学 | Method for solidifying and cementing heavy metal cobalt-containing industrial waste residue by using staphylococcus cohnii ureolytic subspecies |
| CN108675585A (en) * | 2018-06-19 | 2018-10-19 | 浙江工业大学 | Method for solidifying and cementing heavy metal-containing tannery sludge by using bacillus pasteurii |
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| CN110204289B (en) * | 2019-06-19 | 2021-10-22 | 重庆大学 | Method for solidifying tailing sand based on active magnesium oxide microorganisms and product thereof |
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