CN114525220B - Kitchen waste biological decrement strain and application thereof - Google Patents
Kitchen waste biological decrement strain and application thereof Download PDFInfo
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
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- Virology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Environmental & Geological Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention relates to a kitchen waste biological decrement strain and application thereof, wherein the kitchen waste biological decrement strain is obtained by primary screening of a separation culture medium and secondary screening of an enzyme production culture medium, and is identified as bacillus xylolyticus (Geobacillus caldoxylosilyticus) which is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of NO: m20211626, said bacillus xylolyticus cctccc NO: m20211626 is high-temperature resistant and high-salt resistant, has the capability of rapidly and efficiently degrading organic matters in kitchen waste, can be applied to the biological reduction treatment process of the kitchen waste, and realizes rapid biological reduction and stabilization of the kitchen waste.
Description
Technical Field
The invention belongs to the technical field of environmental microorganisms, and particularly relates to a kitchen waste biological decrement strain and application thereof.
Background
Kitchen waste is waste of people after meals (such as peeled vegetable leaves and vegetables) and is mainly sourced from restaurant industry or families such as dining halls of restaurants, enterprises and institutions. With the improvement of the living standard of people in China and the continuous promotion of the urban process, the yield of kitchen wastes is increased year by year. Especially after the garbage classification is implemented in 2019 in China, the yield of kitchen garbage reaches 1.21 hundred million tons. The main components of the kitchen waste are rice, cooked wheaten food, meat and bones, vegetables, grease and the like. The chemical composition mainly comprises proteins, fat, carbohydrate, volatile fatty acid, inorganic salt, trace elements, moisture and the like. Because the kitchen waste has the characteristics of high oil, high salt and the like, the traditional kitchen waste treatment technology such as landfill, incineration, animal feed and crushing and direct discharge is used, and the kitchen waste is harmful to the ecological system and human health. And along with the gradual increase of the yield of the kitchen waste, the influence of the kitchen waste on the environment, economy and society is more remarkable, so that the aim of realizing harmless and reduction treatment of the kitchen waste is urgent.
At present, the common kitchen waste treatment technology at home and abroad comprises drying incineration, sanitary landfill, anaerobic digestion, aerobic composting, ecological feed and the like. The incineration and landfill are low in treatment cost and simple in technology, and are still kitchen waste treatment modes in most cities in China. However, the problems of toxic gases such as dioxin and the like generated by incineration and the problem of groundwater pollution caused by percolate brought by landfill lead the incineration and the landfill to gradually exit from the history stage. Meanwhile, anaerobic digestion is a mainstream treatment technology of kitchen waste in recent years due to the advantages of energy product generation, easy control of malodorous gas emission and the like. However, the anaerobic digestion process has the problems of high requirement of microorganisms on pH value, relatively complex treatment technology, long hydraulic retention time, biogas slurry and slag generation and other secondary pollution. In general, the existing kitchen waste treatment still has the problems of low treatment efficiency, resource waste, serious secondary pollution and the like.
In recent years, a biological reduction technology of kitchen waste has been attracting attention. The kitchen waste biological reduction technology is to decompose organic matters in kitchen waste by utilizing microorganisms so as to achieve the aim of reducing the kitchen waste. Kitchen waste reduction is usually carried out under high-temperature aerobic conditions, and unlike composting and biological drying, the kitchen waste biological reduction is more focused on the reduction of dry matters of the kitchen waste, and aims to maximize the reduction of the kitchen waste. However, the problems of slow starting, poor reduction effect and the like still exist in the biological reduction of the kitchen waste at present, and the main reasons for the problems are that the indigenous microorganisms in the raw materials have low high temperature, high-efficiency functional microorganisms, low concentration and poor activity. Therefore, screening functional microorganism strains with high temperature resistance and strong degradation capacity, and realizing the rapid and efficient biological reduction treatment of kitchen wastes becomes a problem to be solved urgently.
Disclosure of Invention
After a great deal of intensive research, the inventor provides a kitchen waste biological reduction strain, a liquid microbial agent, a preparation method of the liquid microbial agent, a solid microbial agent and a preparation method of the solid microbial agent, and application of the strain in kitchen waste biological reduction.
First aspect:
the invention is verified by separation medium preliminary screening, enzyme production medium rescreening and kitchen waste biological decrement experiments, a high-efficiency kitchen waste biological decrement strain is obtained, which is named Geobacillus caldoxylosilyticus FWGC1, and the strain is gram positive bacteria and rod-shaped through comprehensive analysis of colony and cell morphology, physiological and biochemical characteristics, 16S rDNA gene sequence determination and other data, the colony morphology is irregular, faint yellow, irregular in edge and unsmooth in surface, and the classification status of the strain is identified as follows: bacillus xylolyticus (Geobacillus caldoxylosilyticus) is preserved in China center for type culture collection, wherein the preservation date is 2021, 12 months and 15 days, and the preservation number is CCTCC NO: m20211626, the preservation address is the university of Wuhan in Wuhan, china;
preferably, the bacillus xylolyticus cctccc NO: the M20211626 strain contains a gene sequence shown as SEQ ID NO. 1;
preferably, the bacillus xylolyticus cctccc NO: the M20211626 strain has a sodium chloride tolerance concentration of 100g/L or less, and includes any specific values attributed thereto, for example, 0.1g/L, 0.5g/L, 1g/L, 5g/L, 10g/L, 15g/L, 20g/L, 25g/L, 30g/L, 35g/L, 40g/L, 45g/L, 50g/L, 55g/L, 60g/L, 65g/L, 70g/L, 75g/L, 80g/L, 85g/L, 90g/L, 95g/L, 100g/L, and ranges of any two of these specific values, for example, 1 to 100g/L, 10 to 100g/L, 20 to 100g/L, 30 to 100g/L, 50 to 60g/L, 50 to 70g/L, 50 to 80g/L, 90g/L, 95g to 100g/L, and/or 60 to 100g/L;
preferably, the bacillus xylolyticus cctccc NO: the M20211626 strain has a temperature tolerance of 80℃or less, and includes any specific value attributed thereto, for example, 0 ℃,5 ℃, 10 ℃, 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃, 40 ℃, 45 ℃,50 ℃, 55 ℃, 60 ℃, 65 ℃, 70 ℃, 75 ℃, 80 ℃, and any two of these specific values, for example, 20 to 80 ℃, 30 to 80 ℃, 40 to 80 ℃,50 to 70 ℃,50 to 80 ℃, 60 to 70 ℃, 60 to 80 ℃, 70 to 80 ℃ or 75 to 80 ℃;
preferably, the bacillus xylolyticus cctccc NO: the degradation rate of the M20211626 strain on organic matters of the kitchen waste is more than 76% by weight.
Second aspect:
a liquid microbial inoculant comprising bacillus xylolyticus cctccc NO: m20211626, bacillus xylolyticus cctccc NO: m20211626 has a concentration of at least 1×10 8 cfu/mL, which includes any specific point value attributed thereto, e.g., 1X 10 8 cfu/mL、2×10 8 cfu/mL、3×10 8 cfu/mL、5×10 8 cfu/mL, also includes a range of any two of these specific point values, e.g., 1X 10 8 ～5×10 8 cfu/mL、3×10 8 ～5×10 8 cfu/mL；
Third aspect:
the preparation method of the liquid microbial inoculum comprises the following steps: subjecting the bacillus xylolyticus cctccc NO: the M20211626 strain is inoculated in 5-15 mL of liquid culture medium, preferably 10mL of liquid culture medium, cultured for 16-24 h, more preferably 20h at 48-52 ℃ preferably 50 ℃ and 150-200 r/min preferably 180r/min, then inoculated in the same liquid culture medium for expansion culture at the inoculation amount of 0.5-2% preferably 1% by weight, and cultured for 16-24 h, more preferably 20h at 48-52 ℃ preferably 50 ℃ and 150-200 r/min preferably 180r/min, so that the liquid microbial inoculum of the strain can be obtained;
preferably, the composition of the liquid medium is: peptone 3g/L, yeast extract 2g/L, naCl g/L, mgCl 2 0.125g/L、CaCl 2 0.5g/L、FeSO 4 ·7H 2 O0.01 g/L, microelement solution (Na 2 MoO 4 12g/L、MnCl 2 5g/L、ZnSO 4 ·7H 2 O 0.6g/L、CuSO 4 ·5H 2 O 0.15g/L、CoCl 2 ·6H 2 O 8g/L、NiCl 2 ·6H 2 O0.2g/L)100μLand/L, and the pH value is adjusted to 7-7.5.
Fourth aspect:
a solid microbial inoculum is prepared from the bacillus xylolyticus CCTCC NO: the liquid microbial inoculum of M20211626 consists of a carrier;
preferably, the carrier is selected from at least one of wood chips, rice hulls, wheat bran, and the like.
Fifth aspect:
the preparation method of the solid microbial inoculum comprises the following steps: mixing the carrier with the liquid microbial inoculum according to the weight ratio of 1:0.1-1:5, and drying;
preferably, the range 1:0.1 to 1:5 includes any specific point value attributed thereto, for example, 1:0.1, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.7, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, and also includes a range of any two of these specific point values, for example, 1:0.1 to 1:3, 1:0.1 to 1:4; 1:0.2-1:5, 1:0.5-1:5 or 1:1-1:5.
Sixth aspect:
the above-mentioned Bacillus xylolyticus CCTCC NO: application of M20211626 in biological reduction of kitchen waste;
preferably, the kitchen waste comprises food waste, kitchen waste, fruit and vegetable waste and the like;
preferably, the bacillus xylolyticus cctccc NO: the application of M20211626 in the biological reduction of kitchen waste comprises the following steps: uniformly mixing the solid microbial inoculum with the kitchen waste, and then carrying out aerobic fermentation, wherein the process is regularly ventilated and stirred so as to ensure that the temperature of the materials is uniform and the materials are carried out under an aerobic condition;
preferably, the weight ratio of the solid microbial inoculum to the kitchen waste is 1:1-1:5, and the range includes any specific point value belonging to the range, such as 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5 and 1:5, and also includes the range formed by any two point values of the specific point values, such as 1:1-1:2, 1:1-1:3, 1:1-1:4, 1:2-1:5, 1:3-1:5 or 1:4-1:5;
preferably, the temperature of the aerobic fermentation is 50-80 ℃, which includes any specific point value belonging thereto, such as 50 ℃, 55 ℃, 60 ℃, 65 ℃, 70 ℃, 75 ℃, 80 ℃, and also includes a range consisting of any two of these specific point values, such as 50-70 ℃, 50-80 ℃, 60-70 ℃, 60-80 ℃, 70-80 ℃ or 75-80 ℃;
preferably, the water content of the aerobically fermented material is 40-60% by weight, this range includes any specific point value attributed thereto, for example 40%, 45%, 50%, 55%, 60%, and also includes a range constituted by any two of these specific point values, for example 40-50%, 43-60%, 45-55%, 45-60%, 50-60%, 52-60% or 55-60%.
Compared with the prior art, the invention has the following beneficial effects:
(1) The bacillus xylolyticus CCTCC NO provided by the invention is characterized in that: the M20211626 strain has high salt resistance and can degrade food waste with high salt content;
(2) The bacillus xylolyticus CCTCC NO provided by the invention is characterized in that: m20211626 strain has high temperature resistance and can rapidly degrade organic matters in kitchen waste at high temperature (50-80 ℃).
(3) The bacillus xylolyticus CCTCC NO provided by the invention is characterized in that: the M20211626 strain also has the capability of high-yield protease, amylase and cellulase under the high-temperature condition, and can efficiently degrade the organic matters such as protein, starch, cellulose and the like in the organic solid waste.
Therefore, the bacillus xylolyticus CCTCC NO provided by the invention: the M20211626 strain can be applied to the biological reduction treatment of the kitchen waste in an intensified manner, solves the problems of low biological reduction rate, long treatment time and the like of the kitchen waste, and realizes the reduction and stabilization of the kitchen waste.
Drawings
FIG. 1 shows B.xylolyticus CCTCC NO: phylogenetic tree of the M20211626 strain;
FIG. 2 shows B.xylolyticus CCTCC NO: the M20211626 strain changes the degradation rate of organic matters of the food waste;
FIG. 3 shows B.xylolyticus CCTCC NO: the M20211626 strain changes the degradation rate of organic matters in kitchen waste;
FIG. 4 shows B.xylolyticus CCTCC NO: the M20211626 strain changes the degradation rate of organic matters in the fruit and vegetable garbage;
FIG. 5 shows B.xylolyticus CCTCC NO: the M20211626 strain changes the degradation rate of organic matters in the mixed kitchen waste.
Detailed Description
The invention is further described below by means of specific examples. The present invention is not limited to the following embodiments, and those skilled in the art can easily understand the variations within the scope of the present invention.
The following are specific examples of the present invention, in which the following media were used:
separating and purifying the culture medium: peptone 3g/L, yeast extract 2g/L, naCl g/L, mgCl 2 0.125g/L、CaCl 2 0.5g/L、FeSO 4 ·7H 2 O0.01 g/L, microelement solution (Na 2 MoO 4 12g/L、MnCl 2 5g/L、ZnSO 4 ·7H 2 O 0.6g/L、CuSO 4 ·5H 2 O 0.15g/L、CoCl 2 ·6H 2 O 8g/L、NiCl 2 ·6H 2 O0.2 g/L) 100. Mu.L/L, agar 20g/L, and pH adjusted to 7-7.5.
Seed culture medium: peptone 3g/L, yeast extract 2g/L, naCl g/L, mgCl 2 0.125g/L、CaCl 2 0.5g/L、FeSO 4 ·7H 2 O0.01 g/L, microelement solution (Na 2 MoO 4 12g/L、MnCl 2 5g/L、ZnSO 4 ·7H 2 O0.6g/L、CuSO 4 ·5H 2 O 0.15g/L、CoCl 2 ·6H 2 O 8g/L、NiCl 2 ·6H 2 O0.2 g/L) 100. Mu.L/L, and the pH value is adjusted to 7-7.5.
Salt-tolerant screening media: peptone 3g/L, yeast extract 2g/L, naCl g/L, mgCl 2 0.125g/L、CaCl 2 0.5g/L、FeSO 4 ·7H 2 O0.01 g/L and pH value is regulated to 7-7.5.
Protease screening medium: 10g/L casein, 3g/L peptone, 2g/L, naCl g/L, mgCl yeast extract 2 0.125g/L、CaCl 2 0.5g/L、FeSO 4 ·7H 2 0.01g/L of O, 20g/L of agar and pH value of 7-7.5.
Amylase screening medium: soluble starch 10g/L, peptone 3g/L, yeast extract 2g/L, naCl g/L, mgCl 2 0.125g/L、CaCl 2 0.5g/L、FeSO 4 ·7H 2 0.01g/L of O, 20g/L of agar and pH value of 7-7.5.
Cellulase screening medium: 10g/L sodium carboxymethylcellulose, 3g/L peptone, 2g/L, naCl g/L, mgCl of yeast extract 2 0.125g/L、CaCl 2 0.5g/L、FeSO 4 ·7H 2 0.01g/L of O, 20g/L of agar and pH value of 7-7.5.
Lipase screening medium: 15ml of emulsion (olive oil: polyvinyl alcohol=1:3), 3g/L of peptone, 2g/L, naCl g/L, mgCl of yeast extract 2 0.125g/L、CaCl 2 0.5g/L、FeSO 4 ·7H 2 0.01g/L of O, 20g/L of agar and pH value of 7-7.5.
After the culture medium is prepared, the culture medium is sterilized by high-pressure steam at 121 ℃ for 15min for standby.
Example 1 separation screening and Performance measurement of biological reduction bacteria of kitchen waste
The invention pyrolyzes the bacillus xylolyticus CCTCC NO: isolation and purification process of M20211626 strain: collecting test samples from a high-temperature aerobic fermentation tank of a restaurant garbage treatment plant, adding sterile water, uniformly mixing, carrying out gradient dilution, taking 200 mu L of diluent with different gradients, coating on a separation and purification culture medium flat plate, culturing for 16-24 hours in a constant-temperature incubator at 50 ℃, selecting single bacterial colonies with different forms and sizes, marking, purifying, numbering and preserving, and carrying out primary screening to obtain 41 bacterial strains;
inoculating 41 strains obtained by primary screening onto a protease screening culture medium, an amylase screening culture medium, a cellulase screening culture medium and a lipase screening culture medium respectively by a spot grafting method, measuring the enzyme production capacity of the strains, carrying out secondary screening, culturing at 65 ℃ for 72 hours, measuring the colony diameter (D) and the transparent circle diameter (D), calculating D/D, and selecting the strains with strong protein, starch, cellulose and fat degradation capacity;
respectively inoculating 41 strains obtained by primary screening on salt-tolerant screening culture mediums with different salt concentrations (NaCl 50, 100, 150 and 200 g/L), carrying out re-screening on the salt-tolerant capacity of the strain, culturing at 65 ℃ for 72 hours, and then enabling the colony size to be more than or equal to 1mm, and normally growing and breeding the strain, namely, regarding the strain as the sodium chloride with the concentration tolerance, and selecting the strain with higher salt-tolerant capacity;
inoculating 41 strains obtained by primary screening on a separation and purification culture medium respectively, placing the 41 strains in constant temperature incubators at 50, 55, 60, 65, 70, 75, 80 and 85 ℃ respectively, recording the growth conditions of the 41 strains, carrying out secondary screening on the highest growth temperature of the strains, and selecting the strains with high temperature resistance;
combining the three re-screening results to obtain 4 strains of salt-resistant and high-temperature-resistant strains with strong enzyme production capacity;
inoculating 4 strains obtained by re-screening to a seed culture medium, culturing for 24 hours at 50 ℃ at 180r/min, taking 50mL of bacterial liquid, centrifuging, washing with sterile normal saline, re-suspending to 20mL, attaching to 60g of wood dust, inoculating to 120g of food waste, and performing preliminary verification at 60 ℃, wherein the result is that bacillus xylolyticus CCTCC NO: the degradation rate of the organic matters of the M20211626 strain is maximum and reaches 76% in 48 hours;
bacillus xylolyticus CCTCC NO: the highest salt tolerance concentration of the M20211626 strain is 100g/L sodium chloride; bacillus xylolyticus CCTCC NO: the maximum growth temperature of the M20211626 strain is 80 ℃; bacillus xylolyticus CCTCC NO: the M20211626 strain has strong protein, starch and cellulose degradation capability;
bacillus xylolyticus CCTCC NO: the main biological characteristics of the M20211626 strain are gram-positive bacteria and rod-shaped bacteria, colony forms are irregular, light yellow and irregular in edges and unsmooth in surface, a 16SrDNA sequence of the strain is shown as SEQ ID NO.1, a 16S rDNA sequence is submitted at NCBI, homology sequence comparison analysis is carried out between software and GenBank, MEGA X software is used for constructing a phylogenetic tree of the strain, as shown in figure 1, and CCTCC NO is identified by combining the above information: the M20211626 strain is Bacillus xylolyticus (Geobacillus caldoxylosilyticus).
Example 2 kitchen waste biological reduction and pyrolysis of bacillus xylophilus cctccc NO: preparation of microbial inoculum of M20211626 strain
Preparing a liquid microbial inoculum: cctccc NO: inoculating M20211626 pure strain into 10mL seed culture medium, culturing for 16-24 h in a constant temperature shaking table at 50 ℃ and 180r/min, then inoculating into the same liquid culture medium according to the inoculum size of 1% by weight for expansion culture, and culturing for 16-24 h at 50 ℃ and 180r/min to obtain the liquid microbial inoculum of the strain;
preparation of a solid microbial inoculum: the liquid microbial inoculum of the strain is mixed with carriers such as wood chips, rice hulls, wheat bran and the like, and the liquid microbial inoculum is dried to prepare the strain, wherein the mass ratio of the carriers to the liquid microbial inoculum is 1:0.1-1:5.
Example 3 bacillus xylolyticus cctccc NO: biological reduction treatment effect of M20211626 bacterial strain microbial inoculum on single kitchen waste of different sources
Adding bacillus xylolyticus CCTCC NO: the mass ratio of the solid microbial inoculum of the M20211626 strain to the kitchen waste is 1:2, the high-temperature aerobic fermentation is carried out at 60 ℃ after uniform stirring, ventilation, stirring and proper water supplementing are needed in the process, the water content of the material is ensured to be 40-60%, the heating is ensured to be uniform and the process is carried out under the aerobic condition, and the sample of the treatment system is weighed and calculated every 12 hours, so that the organic matter degradation rate of the kitchen waste is obtained, and the decrement effect is shown as figure 2, figure 3 and figure 4;
food waste is taken from a unit canteen, the organic matter content of which is about 86.3% by dry weight, and fig. 2 shows bacillus xylolyticus cctccc NO: the organic matter degradation rate of M20211626 strain to food waste can be seen from the figure, and bacillus xylolyticus CCTCC NO: the organic matter degradation rate of the M20211626 strain on the food waste almost reaches the highest dry weight ratio 77.79% in 48 hours, the maximum organic matter degradation rate of the control group is 45.68% in dry weight ratio, and the experimental group is improved by 70.29% compared with the control group, so that the bacillus xylolyticus CCTCC NO: m20211626 can obviously improve the reduction effect of the food waste;
kitchen waste is taken from a unit canteen, the organic matter content of the kitchen waste is about 82.84% by dry weight, and fig. 3 shows bacillus xylolyticus CCTCC NO: the organic matter degradation rate of M20211626 strain to kitchen waste can be seen from the figure, bacillus xylolyticus CCTCC NO: the organic matter degradation rate of the M20211626 strain to the food waste reaches the highest dry weight ratio 68.45% in 36h, the maximum organic matter degradation rate of the control group is 45.27%, and the experimental group is improved by 51.2% compared with the control group, so that the bacillus xylolyticus CCTCC NO: the M20211626 strain can obviously improve the reduction effect of kitchen waste;
fruit and vegetable waste is obtained from a farmer market in Shanghai city, and has an organic matter content of about 85.26% by dry weight, and FIG. 4 shows Bacillus xylolyticus CCTCC NO: the organic matter degradation rate of M20211626 strain to fruit and vegetable garbage can be seen from the figure, and bacillus xylolyticus CCTCC NO: m20211626 has the organic matter degradation rate of 46.38% of the dry weight of the highest value in 48h, and the maximum organic matter degradation rate of 38.98% of the dry weight of the control group, and the experimental group is improved by 18.98% compared with the control group, thus, the bacillus xylolyticus CCTCC NO: m20211626 can obviously improve the reduction effect of the fruit and vegetable garbage;
in summary, the bacillus xylolyticus cctccc NO: m20211626 can realize the quick reduction of single kitchen garbage to reach kitchen garbage reduction, effect of stabilization.
Example 4 bacillus xylolyticus cctccc NO: biological reduction treatment effect of M20211626 microbial inoculum on mixed kitchen waste
Mixing three raw materials of food waste to be treated, kitchen waste and fruit and vegetable waste according to a mass ratio of 1:1:1, and adding bacillus xylolyticus CCTCC NO: m20211626 solid microbial inoculum, the mass ratio of the solid microbial inoculum to the mixed raw materials is 1:3, and after uniform stirring, aerobic fermentation is carried out at 60 ℃, ventilation, stirring and proper water supplementing are needed in the process, the water content of the materials is ensured to be 40-60%, so that uniform heating is ensured, and the aerobic fermentation is carried out under an aerobic condition. Weighing and calculating samples of the treatment system every 12 hours to obtain the organic matter degradation rate of the mixed raw materials, wherein the reduction effect is shown in figure 5, and the bacillus xylolyticus CCTCC NO is accessed at 36 hours: the organic matter degradation rate of the experimental group of M20211626 is up to 78.32% by dry weight, and reaches the maximum value in 36h, while the organic matter degradation rate of the control group without inoculating strain is up to 68.32% by dry weight, compared with the control group, bacillus xylolyticus CCTCC NO: m20211626 has improved the organic matter degradation rate of the mixed raw material by 14.64%, thus it can be seen that the bacillus xylolyticus CCTCC NO: m20211626 can obviously improve the decrement effect of the mixed kitchen waste raw materials, and has high application value and wide application prospect.
Sequence listing
<110> Shanghai Kaiki science and technology Co., ltd
<120> a kitchen waste biological decrement strain and application thereof
<141> 2022-01-28
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1435
<212> DNA
<213> Bacillus xylosus pyrolysis (Geobacillus caldoxylosilyticus)
<400> 1
gcctaataca tgcagtcgag cggaccgagc aggagcttgc tcttgtttgg ttagcggcgg 60
acgggtgagt aacacgtggg taacctaccc gtaagaccgg gataactccg ggaaaccgga 120
gctaataccg gataacacca aagaccgcat ggtctttggt tgaaaggcgg cttcggctgt 180
cacttacgga tgggcccgcg gcgcattagc tagttggtga ggtaacggct caccaaggcg 240
acgatgcgta gccggcctga gagggtgacc ggccacactg ggactgagac acggcccaga 300
ctcctacggg aggcagcagt agggaatctt ccgcaatgga cgaaagtctg acggagcgac 360
gccgcgtgag cgaagaaggt cttcggatcg taaagctctg ttgttaggga agaagaagta 420
ccgttcgaat agggcggtac ggtgacggta cctaacgaga aagccccggc taactacgtg 480
ccagcagccg cggtaatacg tagggggcga gcgttgtccg gaattattgg gcgtaaagcg 540
cgcgcaggcg gtcccttaag tctgatgtga aagcccacgg ctcaaccgtg gagggtcatt 600
ggaaactggg ggacttgagt gcagaagagg agagcggaat tccacgtgta gcggtgaaat 660
gcgtagagat gtggaggaac accagtggcg aaggcggctc tctggtctgt aactgacgct 720
gaggcgcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac 780
gatgagtgct aagtgttaga ggggtcaaac cctttagtgc tgtagctaac gcgttaagca 840
ctccgcctgg ggagtacggc cgcaaggctg aaactcaaag gaattgacgg gggcccgcac 900
aagcggtgga gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca 960
tcccctgaca accctggaga cagggcgttc ccccttcggg gggacagggt gacaggtggt 1020
gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1080
cctcgcccct agttgccagc attcagttgg gcactctagg gggactgccg gtgacaaacc 1140
ggaggaaggt ggggatgacg tcaaatcatc atgcccctta tgacctgggc tacacacgtg 1200
ctacaatggg cggtacaaag ggctgcgaac ccgcgagggg gagccaatcc caaaaagccg 1260
ctctcagttc ggattgcagg ctgcaactcg cctgcatgaa gccggaatcg ctagtaatcg 1320
cggatcagca tgccgcggtg aatacgttcc cgggccttgt acacaccgcc cgtcacacca 1380
cgagagcttg caacacccga agtcggtgag gtaacccgca agggagccag ccgcc 1435
Claims (6)
1. Bacillus xylolyticus (Geobacillus caldoxylosilyticus) is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of NO: m20211626.
2. A liquid microbial agent comprising the Bacillus xylolyticus of claim 1, wherein the concentration of the Bacillus xylolyticus is at least 1X 10 8 cfu/mL。
3. The method for preparing the liquid microbial inoculum of claim 2, which is characterized by comprising the following steps: inoculating the bacillus xylolyticus according to claim 1 into a liquid culture medium for culturing for 16-24 hours to obtain the liquid microbial inoculum.
4. A solid microbial inoculant comprising the liquid microbial inoculant of claim 2 and a carrier.
5. The method for preparing the solid microbial inoculum according to claim 4, which is characterized by comprising the following steps:
step one, mixing the carrier and the liquid microbial inoculum according to the weight ratio of 1:0.1-1:5;
and step two, drying the mixture obtained in the step one.
6. The use of bacillus xylolyticus according to claim 1 in the biological reduction of kitchen waste.
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| CN111269860A (en) * | 2020-03-09 | 2020-06-12 | 森诺技术有限公司 | Microbial strain for degrading kitchen waste and application thereof |
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