CN114525203A - Bacterial colony density measuring equipment for beneficial bacteria in feed additive - Google Patents
Bacterial colony density measuring equipment for beneficial bacteria in feed additive Download PDFInfo
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- CN114525203A CN114525203A CN202210155945.5A CN202210155945A CN114525203A CN 114525203 A CN114525203 A CN 114525203A CN 202210155945 A CN202210155945 A CN 202210155945A CN 114525203 A CN114525203 A CN 114525203A
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Abstract
The invention discloses a colony density measuring device for beneficial bacteria in a feed additive, relates to the technical field of colony detection, and solves the problem that the existing device cannot detect the colony density of lactic acid bacteria in the feed additive. A bacterial colony density measuring device for beneficial bacteria in a feed additive comprises a vibrating table, wherein the vibrating table is fixedly connected to the bottom of the inner side of a shading detection bin, the right end of the vibrating table is fixedly connected with a horizontal reciprocating screw rod, the rear end of the shading detection bin is fixedly connected with a spectrophotometric color meter, and the front end of the spectrophotometric color meter is fixedly connected with a light testing part; the ultrasonic transducer is controlled to run by the control part, the reaction vessel at the top of the ultrasonic transducer vibrates, the solution containing lactic acid and litmus reagent are fully mixed and discolored, the color of the discolored reaction vessel is detected by the spectrophotometry color measuring instrument, the color can be converted into the rated lactic acid content through the chromaticity value, the lactic acid bacteria density is converted into the lactic acid bacteria density through the rated lactic acid content, and the bacterial colony density of the lactic acid bacteria in the feed additive can be obtained.
Description
Technical Field
The invention relates to the technical field of colony detection, in particular to a colony density measuring device for beneficial bacteria in a feed additive.
Background
In the feed production process, beneficial bacteria such as lactobacillus need to be added into the feed, the absorption of protein, monosaccharide, calcium, magnesium and other nutrient substances by livestock can be promoted, a large amount of beneficial substances such as vitamin B group and the like are generated, the breeding rate, the milk yield and the reproduction rate of female livestock can be improved, and equipment capable of detecting the colony density of the lactobacillus in the feed additive is needed.
Based on the above-mentioned, the bacterial colony densitometry of traditional beneficial bacterium is comparatively loaded down with trivial details, it can turn into rated lactic acid content through chromaticity numerical value not to set up, the lactic acid bacteria density is turned into to the rated lactic acid content of rethread, the structure of the bacterial colony density of lactic acid bacteria among the feed additive comes to be reachd, it is convenient for carry out the structure that temperature compensation kept to culture solution not to set up, do not set up the structure that can form anaerobic environment fast, be not convenient for cultivate in order to detect the interior lactic acid bacteria bacterial colony of bucket, do not set up the structure that can sieve out impurity in the feed additive, be not convenient for further detect.
Therefore, the existing requirements are not met, and an apparatus for determining the colony density of beneficial bacteria in the feed additive is provided.
Disclosure of Invention
Problem (A)
The invention aims to provide a device for measuring colony density of beneficial bacteria in a feed additive, and the device is used for solving the problems that the traditional colony density of beneficial bacteria proposed in the background technology is complicated to measure, the colony density can be converted into the rated lactic acid content through a chromaticity numerical value and then converted into the lactic acid bacteria density through the rated lactic acid content to obtain the colony density of lactic acid bacteria in the feed additive, a structure which is convenient for temperature compensation and maintenance of a culture solution is not arranged, a structure which can quickly form an anaerobic environment is not arranged, the colony of lactic acid bacteria in a barrel is inconvenient to culture for detection, a structure which can screen out impurities in the feed additive is not arranged, and the detection is inconvenient.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: a colony density measuring device for beneficial bacteria in feed additives comprises a workbench; the workbench comprises an insulation can and front support columns, the top of the workbench is fixedly connected with the insulation can and fixedly connected to the left side of the top of the workbench, the number of the front support columns is two, and the front support columns are fixedly connected to the right side of the top of the workbench; the right side of the top of the workbench is fixedly connected with a control part; the feed additive processing structure comprises a top frame, the top frame is fixedly connected to the top end of the workbench, a crushed aggregate reciprocating screw is fixedly connected to the top of the top frame, a servo motor is fixedly connected to the front end of a ball nut seat of the crushed aggregate reciprocating screw, a grinding disc is arranged at the bottom of a rotating shaft of the servo motor through coaxial connection, and spiral grains are fixedly connected to the bottom surface of the grinding disc; a bacterial colony culture structure is arranged in the heat preservation box; the front end of the heat insulation box is provided with a conversion inspection structure, the conversion inspection structure comprises a shading detection bin, and the shading detection bin is fixedly connected to the top of the front end of the heat insulation box.
Preferably, the feed additive processing structure further comprises: the fixed hoop is fixedly connected to the vertical surface of the front end of the top frame; the mixing funnel pipe is connected and arranged on the inner side of the fixed hoop through a hoop; and the vertical pipe is fixedly connected to the bottom of the mixing funnel pipe.
Preferably, the feed additive processing structure further comprises: the multilayer filter screens are connected and arranged on the inner side of the vertical pipe through threads; the electromagnetic valve A is arranged at the bottom of the vertical pipe in a flange connection mode; the inlet pipe, the inlet pipe passes through flange joint and sets up in solenoid valve A bottom.
Preferably, the colony culture structure further comprises: the diluting barrel is fixedly connected to the inner side of the heat insulation box; the electric heating wire is fixedly connected to the outer curved side surface of the dilution barrel; the thread jackscrew is arranged at the top of the dilution barrel through thread connection; the immersion fluid cage, immersion fluid cage fixed connection is in screw thread jackscrew bottom.
Preferably, the transformation assay structure further comprises: the dilution pump is fixedly connected to the top surface of the insulation can; dilution pipe, dilution pipe fixed connection are in dilution pump left end, and the dilution pipe left end is perpendicular to be worn to locate inside the dilution bucket downwards.
Preferably, the transformation assay structure further comprises: the mixing pipe is fixedly connected to the top of the dilution pipe; the electromagnetic valve B is arranged in the mixing pipe through flange connection; the liquid inlet pipe is fixedly connected to the rear end of the dilution pump.
Preferably, the transformation assay structure further comprises: the guide pump is fixedly connected to the top surface of the insulation can; the pipette is fixedly connected to the left end of the diversion pump, and the left end of the pipette vertically penetrates into the dilution barrel downwards; the liquid guide pipe is fixedly connected to the front end of the guide pump, and the electromagnetic valve C is arranged in the liquid guide pipe through flange connection.
Preferably, the conversion inspection structure is still including the shaking table, shaking table fixed connection is in shading detection storehouse inboard bottom, shaking table front end fixedly connected with ultrasonic transducer, shaking table right-hand member fixedly connected with horizontal reciprocating screw, horizontal reciprocating screw ball nut seat top left end fixedly connected with push rod, push rod left end fixedly connected with reaction vessel, shading detection storehouse rear end fixedly connected with spectrophotometry color meter, spectrophotometry color meter front end fixedly connected with portion of testing, the cyclic annular fluorescent tube of portion of testing outside fixedly connected with.
Preferably, shading detects storehouse top fixedly connected with perpendicular reciprocal lead screw, and perpendicular reciprocal lead screw front side is provided with adds the pipe, and perpendicular reciprocal lead screw ball nut seat front end fixedly connected with piston rod, piston rod in adding intraductal sliding connection, add the socle portion and be provided with out the liquid check valve through flange joint, shading detects storehouse top fixedly connected with litmus reagent jar, adds the pipe right side and is connected with litmus reagent jar through the pipeline, and the inside flange joint of continuous pipeline is provided with the feed liquor check valve.
Preferably, the control part is still including temperature sensor and PH sensor, and temperature sensor and PH sensor fixed connection are in the control part left end, and temperature sensor and PH sensor are located the dilution bucket inboard.
(III) advantageous effects
1. The invention can lead litmus reagent in a litmus reagent tank to be led into the reaction vessel below through the adding pipe by atmospheric pressure through the control part by arranging the conversion inspection structure and controlling the operation of the vertical reciprocating screw rod by the control part, controls the operation of the guide pump by the control part, controls the opening of the electromagnetic valve C, can lead the pipette to suck diluted upper solution in the diluting bucket through the operation of the guide pump, leads bacteria-containing solution to be downwards guided into the reaction vessel through the liquid guide pipe, controls the operation of the ultrasonic transducer by the control part, can lead the ultrasonic transducer to do work to generate high-frequency vibration on the vibration table, can lead the reaction vessel at the top of the ultrasonic transducer to generate vibration, can lead the solution containing lactic acid to be fully mixed with the litmus reagent to generate color change reaction, and controls the operation of the horizontal reciprocating screw rod by the control part, can make it drive reaction ware translation to optometry portion below, send the light of rated strength through control division control ring-shaped fluorescent tube, detect the reaction ware colour after discolouring through the spectrophotometry color measuring instrument, can turn into rated lactic acid content through the chromaticity numerical value, turn into lactic acid bacteria density through the lactic acid content that produces in the rated time again, can obtain the bacterial colony density of lactic acid bacteria in the feed additive.
2. The temperature compensation circuit is arranged, the temperature of the feed additive solution in the dilution barrel is detected through the temperature sensor, and when the temperature is lower than forty ℃, the electric heating wire is controlled to operate through the temperature compensation circuit to intermittently heat the dilution barrel, so that the temperature of the solution in the dilution barrel can be kept at forty ℃, and the lactic acid bacteria colony in the barrel can be conveniently cultured.
3. According to the invention, by arranging the bacterial colony culture structure, after the thread jackscrew is vertically placed into the threaded hole in the top of the dilution barrel from top to bottom, after the thread jackscrew is screwed down, the electromagnetic valve A is controlled to be closed by the control part, so that oxygen can be isolated in the dilution barrel, and the immersion liquid cage at the bottom end of the thread jackscrew is immersed in the culture solution, so that the oxidation-reduction reaction can be carried out on active iron particles in the immersion liquid cage and the oxygen, the oxygen can be removed, an anaerobic environment can be formed in the dilution barrel, and the lactic acid bacteria can be conveniently and rapidly propagated to generate lactic acid.
4. The feed additive processing structure is arranged, after the feed additive with rated mass is weighed and taken, the feed additive is placed in the mixing funnel pipe, the operation of the reciprocating screw rod of the crushed aggregates is controlled by the control part, the ball nut seat of the crushed aggregates can drive the servo motor at the front end of the ball nut seat to vertically reciprocate, the servo motor is controlled by the control part to synchronously operate, the feed additive in the mixing funnel pipe can be ground and extruded in the rotating process of the grinding disc, the operation of the dilution pump is synchronously controlled by the control part, after the culture solution is sucked by the liquid inlet pipe of the mixing funnel pipe, the solution can be transmitted into the mixing funnel pipe through the mixing pipe, the ground additive and the culture solution can be quickly mixed, the additive solution is filtered through the multilayer filter screen below the mixing funnel pipe, impurities can be screened, and further detection is facilitated.
Drawings
FIG. 1 is a schematic diagram of an overall right-side top perspective view of an embodiment of the present invention;
FIG. 2 is an enlarged view of a portion of A in FIG. 1 according to a first embodiment of the present invention;
FIG. 3 is a schematic overall disassembled three-dimensional structure of the embodiment of the present invention;
FIG. 4 is an enlarged view of a portion B of FIG. 3 according to one embodiment of the present invention;
FIG. 5 is an enlarged view of a portion of C in FIG. 3 according to one embodiment of the present invention;
FIG. 6 is a schematic side sectional view of a dilution barrel according to an embodiment of the present invention;
FIG. 7 is a schematic front side top perspective view of an embodiment of the present invention;
FIG. 8 is a schematic bottom perspective view of an embodiment of the present invention;
FIG. 9 is a schematic perspective view of a horizontal reciprocating screw according to a second embodiment of the present invention;
FIG. 10 is a schematic perspective exploded view of a temperature sensor according to a third embodiment of the present invention;
in fig. 1 to 10, the correspondence between the part names or lines and the reference numbers is:
1. a work table; 101. a heat preservation box; 102. a front pillar; 2. a control unit; 201. a temperature sensor; 202. a pH sensor; 3. a top frame; 301. a crushed material reciprocating screw rod; 302. a servo motor; 303. a grinding disk; 3031. helical thread; 304. a fixing hoop; 305. a mixing funnel; 306. a vertical tube; 3061. a plurality of layers of filter screens; 307. an electromagnetic valve A; 3071. a feed pipe; 4. a dilution barrel; 401. an electric heating wire; 402. screwing the thread; 4021. an immersion cage; 5. a dilution pump; 501. a dilution tube; 502. a mixing tube; 5021. a solenoid valve B; 503. a liquid inlet pipe; 6. a diversion pump; 601. a pipette; 602. a catheter; 6021. a solenoid valve C; 7. a shading detection bin; 701. a vibration table; 7011. an ultrasonic transducer; 702. a horizontally reciprocating lead screw; 7021. a push rod; 7022. a reaction vessel; 703. a spectrophotometric color meter; 7031. a light inspection part; 7032. an annular lamp tube; 704. a vertically reciprocating lead screw; 705. an addition pipe; 7051. a liquid outlet one-way valve; 7052. a liquid inlet check valve; 706. litmus reagent jar.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1 to 8, according to a first embodiment of the present invention: a colony density measuring device of beneficial bacteria in feed additives comprises a workbench 1; the workbench 1 comprises an insulation box 101 and front pillars 102, the top of the workbench is fixedly connected with the insulation box 101 and is fixedly connected to the left side of the top of the workbench 1, the number of the front pillars 102 is two, the front pillars 102 are fixedly connected to the right side of the top of the workbench 1, the top of the front pillars 102 is fixed with the bottom surface of the shading detection bin 7, and the shading detection bin 7 can be supported; the right side of the top of the workbench 1 is fixedly connected with a control part 2; the top of the workbench 1 is provided with a feed additive processing structure, the feed additive processing structure comprises a top frame 3, the top frame 3 is fixedly connected to the top of the workbench 1, the top of the top frame 3 is fixedly connected with a crushed aggregate reciprocating screw 301, the front end of a ball nut seat of the crushed aggregate reciprocating screw 301 is fixedly connected with a servo motor 302, the servo motor 302 is electrically connected with the control part 2, the bottom of a rotating shaft of the servo motor 302 is provided with a grinding disc 303 through coaxial connection, and the bottom surface of the grinding disc 303 is fixedly connected with spiral grains 3031; a bacterial colony culture structure is arranged in the heat preservation box 101; the front end of the heat preservation box 101 is provided with a conversion inspection structure, the conversion inspection structure comprises a shading detection bin 7, and the shading detection bin 7 is fixedly connected to the top of the front end of the heat preservation box 101.
As shown in fig. 6, the feed additive processing structure further comprises: the fixed hoop 304, the fixed hoop 304 is fixedly connected to the front end vertical surface of the top frame 3; the mixing funnel pipe 305 is arranged on the inner side of the fixed hoop 304 through a hoop connection; a vertical pipe 306, the vertical pipe 306 being fixedly connected to the bottom of the mixing funnel 305; a multi-layer filter net 3061, the multi-layer filter net 3061 being disposed inside the vertical pipe 306 by a threaded coupling; the electromagnetic valve A307 is arranged at the bottom of the vertical pipe 306 through flange connection, and the electromagnetic valve A307 is electrically connected with the control part 2; a feeding pipe 3071, the feeding pipe 3071 is arranged at the bottom end of the electromagnetic valve A307 through flange connection, after weighing and taking the feed additive with rated mass, the feed additive is placed in the mixing funnel pipe 305, the operation of the reciprocating lead screw 301 of the crushed aggregates is controlled by the control part 2, the servo motor 302 at the front end of the reciprocating lead screw can be driven by the ball nut seat to vertically reciprocate, the feed additive in the mixing funnel pipe 305 can be ground and extruded in the rotating process of the grinding disc 303 through the synchronous operation of the servo motor 302 controlled by the control part 2, the operation of the dilution pump 5 is synchronously controlled by the control part 2, after the culture solution is sucked by the liquid inlet pipe 503, the solution can be transmitted into the mixing funnel pipe 305 through the mixing pipe 502, the ground additive and the culture solution can be rapidly mixed, the additive solution is filtered through the multilayer filter screen 3061 below the mixing funnel pipe 305, and the impurities can be screened out, the mixed liquid can be made to flow into the inside of the dilution barrel 4 below.
As shown in FIG. 6, the colony culture structure further comprises: the diluting barrel 4 is fixedly connected to the inner side of the heat insulation box 101; the heating wire 401 is fixedly connected to the outer curved side surface of the dilution barrel 4, and the electric property of the heating wire 401 is connected with the control part 2; the thread top thread 402 is arranged at the top of the dilution barrel 4 through thread connection; immersion fluid cage 4021, immersion fluid cage 4021 fixed connection is in screw thread jackscrew 402 bottom, the inside active iron granule that is provided with of immersion fluid cage 4021, through with screw thread jackscrew 402 from the top down place into the screw hole at 4 tops of dilution bucket after, through screwing up screw thread jackscrew 402 after, close through control division 2 control solenoid valve A307, can make the inside isolated oxygen of dilution bucket 4, the immersion fluid cage 4021 through screw thread jackscrew 402 bottom soaks in the culture solution, can make the inside active iron granule of immersion fluid cage 4021 and oxygen take place redox reaction, can get rid of oxygen, can make the inside anaerobic environment that forms of dilution bucket 4, the lactic acid bacteria rapid propagation of being convenient for produces lactic acid.
As shown in fig. 2, the transformation test structure further includes: the dilution pump 5 is fixedly connected to the top surface of the insulation can 101, and the electrical property of the dilution pump 5 is connected with the control part 2; the dilution pipe 501, the dilution pipe 501 is fixedly connected to the left end of the dilution pump 5, the left end of the dilution pipe 501 vertically penetrates through the interior of the dilution barrel 4 downwards; the mixing pipe 502 is fixedly connected to the top of the dilution pipe 501, and the left end of the mixing pipe 502 is fixedly connected to the inner side of the mixing funnel pipe 305; the electromagnetic valve B5021 and the electromagnetic valve B5021 are arranged inside the mixing pipe 502 through flange connection, and the electrical property of the mixing pipe is connected with the control part 2; the liquid inlet pipe 503 is fixedly connected to the rear end of the dilution pump 5; the guide pump 6 is fixedly connected to the top surface of the heat preservation box 101, and the electric property of the guide pump 6 is connected with the control part 2; the pipette 601, the pipette 601 is fixedly connected to the left end of the guide pump 6, and the left end of the pipette 601 vertically penetrates into the diluting barrel 4 downwards; the catheter 602 is fixedly connected to the front end of the guide pump 6, the electromagnetic valve C6021 is arranged in the catheter 602 through flange connection, the guide pump 6 is controlled to operate through the control part 2, the cultured solution can be guided, and detection is facilitated.
Example two: as shown in fig. 9, the conversion and inspection structure further includes a vibration table 701, the vibration table 701 is fixedly connected to the bottom of the inner side of the light-shielding detection bin 7, the front end of the vibration table 701 is fixedly connected with an ultrasonic transducer 7011, the electrical property of the ultrasonic transducer is connected with the control part 2, the right end of the vibration table 701 is fixedly connected with a horizontal reciprocating lead screw 702, the electrical property of the horizontal reciprocating lead screw is connected with the control part 2, the left end of the top of a ball nut seat of the horizontal reciprocating lead screw 702 is fixedly connected with a push rod 7021, the left end of the push rod 7021 is fixedly connected with a reaction vessel 7022, the rear end of the light-shielding detection bin 7 is fixedly connected with a spectrophotometric color measuring instrument 703, the front end of the spectrophotometric color measuring instrument 703 is fixedly connected with an optometry part 7031, and the outer side of the optometry part 7031 is fixedly connected with an annular lamp tube 7032; the top of the shading detection bin 7 is fixedly connected with a vertical reciprocating screw 704, the electrical property of the vertical reciprocating screw 704 is connected with the control part 2, the front side of the vertical reciprocating screw 704 is provided with an adding pipe 705, the front end of a ball nut seat of the vertical reciprocating screw 704 is fixedly connected with a piston rod, the piston rod is connected with the inner side of the adding pipe 705 in a sliding way, the bottom of the adding pipe 705 is provided with a liquid outlet one-way valve 7051 through a flange connection, the top of the shading detection bin 7 is fixedly connected with a litmus reagent tank 706, the right side of the adding pipe 705 is connected with the litmus reagent tank 706 through a pipeline, the inner side of the pipeline is provided with a liquid inlet one-way valve 7052 through a flange connection, the ball nut seat can drive a piston cylinder to carry out piston motion on the inner side of the adding pipe 705 through the control part 2, the litmus reagent in the litmus reagent tank 706 can be led into the reaction vessel 7022 below through the adding pipe 705 through atmospheric pressure, the control part 2 controls the flow guide pump 6 to operate, meanwhile, the electromagnetic valve C6021 is controlled to be opened, the liquid suction pipe 601 can suck the diluted upper solution in the dilution barrel 4 by operating the guide pump 6, the bacteria-containing solution can be downwards guided into the reaction vessel 7022 by the guide pipe 602, the ultrasonic transducer 7011 is controlled to operate by the control part 2, the vibration table 701 can do work to generate high-frequency vibration, the reaction vessel 7022 at the top of the reaction vessel can generate vibration, the solution containing lactic acid and litmus reagent can be fully mixed, the horizontal reciprocating lead screw 702 is controlled to operate by the control part 2, the reaction vessel 7022 can be driven to move to the position below the light testing part 7031, the annular lamp tube 7032 is controlled by the control part 2 to emit light with rated intensity, the color of the reaction vessel 7022 after color change is detected by the spectrophotometer 703, the content of the color can be converted into the content of the rated lactic acid, and then the content of the rated lactic acid is converted into the density of lactic acid bacteria, the colony density of the lactic acid bacteria in the feed additive can be obtained.
Example three: as shown in fig. 10, the control part 2 further includes a temperature sensor 201 and a PH sensor 202, the temperature sensor 201 and the PH sensor 202 are fixedly connected to the left end of the control part 2, and the temperature sensor 201 and the PH sensor 202 are located inside the dilution barrel 4; the inside temperature compensation circuit that is provided with of control part 2, temperature compensation circuit electrical property is connected with heating wire 401 and temperature sensor 201, detect the back through temperature sensor 201 to the inside feed additive solution temperature of dilution bucket 4, when the temperature is less than forty degrees, through the operation of temperature compensation circuit control heating wire 401, make it carry out intermittent type formula heating to dilution bucket 4, can make the solution temperature of dilution bucket 4 inside keep forty degrees, be convenient for cultivate the lactic acid bacteria colony in the bucket.
The working principle is as follows: when the device is used, after weighing and taking feed additives with rated mass, the feed additives are placed in the mixing funnel pipe 305, the operation of the reciprocating screw 301 of crushed aggregates is controlled by the control part 2, the ball nut seat of the crushed aggregates can drive the servo motor 302 at the front end of the ball nut seat to vertically reciprocate, the synchronous operation of the servo motor 302 is controlled by the control part 2, the feed additives in the mixing funnel pipe 305 can be ground and extruded in the rotating process of the grinding disc 303, the operation of the dilution pump 5 is synchronously controlled by the control part 2, after the culture solution is sucked by the liquid inlet pipe 503, the solution can be transmitted into the mixing funnel pipe 305 through the mixing funnel pipe 502, the additives in grinding can be rapidly mixed with the culture solution, the additive solution is filtered by the multilayer filter screen 3061 below the mixing funnel pipe 305, impurities can be screened, and the mixed solution can flow into the dilution barrel 4 below, by starting the operation of the dilution pump 5, the culture solution can be poured into the dilution barrel 4 through the dilution pipe 501, after the temperature of the feed additive solution in the dilution barrel 4 is detected through the temperature sensor 201, when the temperature is lower than forty ℃, the operation of the electric heating wire 401 is controlled through the temperature compensation circuit, the intermittent heating is carried out on the dilution barrel 4, the temperature of the solution in the dilution barrel 4 can be kept at forty ℃, after the threaded jackscrew 402 is vertically placed into the threaded hole at the top of the dilution barrel 4 from top to bottom, after the threaded jackscrew 402 is screwed down, the electromagnetic valve A307 is controlled to be closed through the control part 2, the oxygen can be isolated in the dilution barrel 4, the immersion cage 4021 at the bottom end of the threaded jackscrew 402 is immersed in the culture solution, the oxidation-reduction reaction can be carried out between the active iron particles in the immersion cage 4021 and the oxygen, the oxygen can be removed, and the anaerobic environment can be formed in the dilution barrel 4, the rapid propagation of lactic acid bacteria is facilitated to produce lactic acid, the electric heating wire 401 is controlled at regular time by the control part 2 to do work to preserve heat of the dilution barrel 4, the solution in the barrel is preserved for forty-eight hours at regular time, the guide pump 6 is controlled at regular time by the control part 2 to operate, the electromagnetic valve C6021 is controlled to be opened at the same time, the guide pump 6 operates to enable the pipette 601 to suck the diluted upper solution in the dilution barrel 4, the bacteria-containing solution can be guided downwards into the reaction vessel 7022 through the guide pipe 602, the ultrasonic transducer 7011 is controlled by the control part 2 to operate to enable the ultrasonic transducer to do work to the vibration table 701 to generate high-frequency vibration, the reaction vessel 7022 at the top of the pipette can generate vibration, the solution containing lactic acid and litmus reagent can be fully mixed, the horizontal reciprocating lead screw 702 is controlled by the control part 2 to operate, the reaction vessel 7022 can be driven to translate to the part below the light inspection part 7031, the annular lamp 7032 is controlled by the control part 2 to emit light with rated intensity, the color of the reaction vessel 7022 after the color change is detected by a spectrophotometric color measuring instrument 703, and the color can be converted into the rated lactic acid content through the chromaticity value, and then converted into the lactic acid bacteria density through the rated lactic acid content, so that the colony density of the lactic acid bacteria in the feed additive can be obtained.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (10)
1. A bacterial colony density measuring equipment of beneficial bacteria in feed additive is characterized in that: comprises a workbench (1); the workbench (1) comprises an insulation can (101) and front pillars (102), the top of the workbench is fixedly connected with the insulation can (101) and is fixedly connected to the left side of the top of the workbench (1), the number of the front pillars (102) is two, and the front pillars (102) are fixedly connected to the right side of the top of the workbench (1); the right side of the top of the workbench (1) is fixedly connected with a control part (2); the feed additive processing structure is arranged at the top of the workbench (1), and comprises a top frame (3), wherein the top frame (3) is fixedly connected to the top end of the workbench (1), the top of the top frame (3) is fixedly connected with a crushed aggregate reciprocating screw rod (301), the front end of a ball nut seat of the crushed aggregate reciprocating screw rod (301) is fixedly connected with a servo motor (302), the bottom of a rotating shaft of the servo motor (302) is coaxially connected with a grinding disc (303), and the bottom surface of the grinding disc (303) is fixedly connected with spiral threads (3031); a bacterial colony culture structure is arranged in the heat preservation box (101); the front end of the heat preservation box (101) is provided with a conversion inspection structure, the conversion inspection structure comprises a shading detection bin (7), and the shading detection bin (7) is fixedly connected to the top of the front end of the heat preservation box (101).
2. The apparatus for determining colony density of beneficial bacteria in feed additive according to claim 1, wherein the feed additive processing structure further comprises: the fixed hoop (304), the fixed hoop (304) is fixedly connected to the front end vertical surface of the top frame (3); the mixing funnel pipe (305), the mixing funnel pipe (305) is arranged on the inner side of the fixed hoop (304) through a clamping hoop connection; a vertical pipe (306), the vertical pipe (306) is fixedly connected to the bottom of the mixing funnel pipe (305).
3. The apparatus for determining colony density of beneficial bacteria in feed additive according to claim 2, wherein the feed additive processing structure further comprises: the multi-layer filter screen (3061), the multi-layer filter screen (3061) is arranged at the inner side of the vertical pipe (306) through threaded connection; the electromagnetic valve A (307) is connected and arranged at the bottom of the vertical pipe (306) through a flange; the feeding pipe (3071) is connected with the bottom end of the electromagnetic valve A (307) through a flange, and the feeding pipe (3071) is arranged at the bottom end of the electromagnetic valve A (307).
4. The apparatus for determining colony density of beneficial bacteria in feed additive according to claim 1, wherein the colony culture structure further comprises: the dilution barrel (4), the dilution barrel (4) is fixedly connected to the inner side of the heat preservation box (101); the electric heating wire (401), the electric heating wire (401) is fixedly connected to the outer curved side of the dilution barrel (4); the threaded top thread (402), the threaded top thread (402) is arranged at the top of the dilution barrel (4) in a threaded connection manner; the immersion liquid cage (4021), the immersion liquid cage (4021) is fixedly connected to the bottom end of the screw thread jackscrew (402).
5. The apparatus for determining colony density of beneficial bacteria in feed additive according to claim 1, wherein said transformation test structure further comprises: the dilution pump (5), the dilution pump (5) is fixedly connected to the top surface of the insulation can (101); dilution pipe (501), dilution pipe (501) fixed connection is in dilution pump (5) left end, and dilution pipe (501) left end is inside penetrating through setting up dilution bucket (4) perpendicularly downwards.
6. The apparatus for determining colony density of beneficial bacteria in feed additive according to claim 5, wherein said transformation test structure further comprises: the mixing pipe (502), the mixing pipe (502) is fixedly connected to the top of the dilution pipe (501); the electromagnetic valve B (5021) is arranged inside the mixing pipe (502) in a flange connection mode (5021); the liquid inlet pipe (503) is fixedly connected to the rear end of the dilution pump (5).
7. The apparatus for determining colony density of beneficial bacteria in feed additive according to claim 6, wherein the transformation test structure further comprises: the guide pump (6), the guide pump (6) is fixedly connected with the top surface of the insulation can (101); the liquid suction pipe (601), the liquid suction pipe (601) is fixedly connected to the left end of the diversion pump (6), and the left end of the liquid suction pipe (601) vertically penetrates through the interior of the dilution barrel (4) downwards; the liquid guide pipe (602), the liquid guide pipe (602) is fixedly connected with the front end of the guide pump (6), and the interior of the liquid guide pipe (602) is provided with an electromagnetic valve C (6021) through flange connection.
8. The apparatus for measuring the colony density of beneficial bacteria in a feed additive according to claim 7, wherein the conversion test structure further comprises a vibration table (701), the vibration table (701) is fixedly connected to the bottom of the inner side of the shading test bin (7), an ultrasonic transducer (7011) is fixedly connected to the front end of the vibration table (701), a horizontal reciprocating lead screw (702) is fixedly connected to the right end of the vibration table (701), a push rod (7021) is fixedly connected to the left end of the top of a ball nut seat of the horizontal reciprocating lead screw (702), a reaction dish (7022) is fixedly connected to the left end of the push rod (7021), a spectrophotometric color measuring instrument (703) is fixedly connected to the rear end of the shading test bin (7), a light testing part (7031) is fixedly connected to the front end of the spectrophotometric color measuring instrument (703), and an annular lamp tube (7032) is fixedly connected to the outer side of the light testing part (7031).
9. The apparatus for measuring the colony density of beneficial bacteria in a feed additive according to claim 1, wherein a vertical reciprocating lead screw (704) is fixedly connected to the top of the shading detection bin (7), an adding pipe (705) is arranged on the front side of the vertical reciprocating lead screw (704), a piston rod is fixedly connected to the front end of a ball nut seat of the vertical reciprocating lead screw (704), the piston rod is slidably connected to the inner side of the adding pipe (705), a liquid outlet one-way valve (7051) is arranged at the bottom of the adding pipe (705) through a flange connection, a litmus reagent tank (706) is fixedly connected to the top of the shading detection bin (7), the right side of the adding pipe (705) is connected with the litmus reagent tank (706) through a pipeline, and a liquid inlet one-way valve (7052) is arranged inside the connecting pipeline in a flange connection mode.
10. The apparatus for determining the colony density of beneficial bacteria in a feed additive according to claim 1, wherein the control part (2) further comprises a temperature sensor (201) and a pH sensor (202), the temperature sensor (201) and the pH sensor (202) are fixedly connected to the left end of the control part (2), and the temperature sensor (201) and the pH sensor (202) are positioned inside the dilution barrel (4).
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| CN202210155945.5A CN114525203A (en) | 2022-02-21 | 2022-02-21 | Bacterial colony density measuring equipment for beneficial bacteria in feed additive |
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| CN202210155945.5A CN114525203A (en) | 2022-02-21 | 2022-02-21 | Bacterial colony density measuring equipment for beneficial bacteria in feed additive |
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