CN113984725A

CN113984725A - Detection apparatus for tetracycline residue in meat and fresh milk based on fluorescence probe

Info

Publication number: CN113984725A
Application number: CN202111191613.4A
Authority: CN
Inventors: 胡琼
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-10-13
Filing date: 2021-10-13
Publication date: 2022-01-28

Abstract

The invention discloses a device for detecting tetracycline residues in meat and fresh milk based on a fluorescent probe, relates to the technical field of residue detection, and solves the problem that the existing device cannot fully mix a sample with the fluorescent probe. A detection device for tetracycline residues in meat and fresh milk based on a fluorescent probe comprises a bearing table; a PCR fluorescence quantitative instrument is arranged on the right side of the top surface of the bearing table; the bottom surface of the bearing table is fixedly connected with a pressure pump; a fluorescence detection structure is arranged at the top of the bearing table; a raw material processing mechanism is arranged in the fluorescence detection structure; after the fluorescent probe is injected into the injection pipe, the sealing sleeve cover is controlled to vertically press down through the reciprocating screw rod to seal the decomposition barrel, the top shaft rod can drive the three groups of decomposition wheels to rotate by controlling the operation of the variable speed motor, the sample is convenient to break, and the sample and the fluorescent probe can be fully mixed.

Description

Detection apparatus for tetracycline residue in meat and fresh milk based on fluorescence probe

Technical Field

The invention relates to the technical field of residue detection, in particular to a device for detecting tetracycline residues in meat and fresh milk based on a fluorescent probe.

Background

Tetracycline can be widely distributed in various tissues after being absorbed by livestock and can be deposited in bone and tooth tissues, the binding rate of the tetracycline and plasma protein is about 20% -30%, so that the tetracycline can easily permeate into chest cavity, abdominal cavity, fetal circulation and milk, and women can eat the meat-egg milk containing tetracycline residues, so that the enamel of newborn children at birth is not fully developed and yellow deposits appear, which can cause deformity or growth inhibition, and a large amount of oral administration for a long time can cause serious liver damage and can also aggravate the original renal insufficiency, which can influence amino acid metabolism to increase azotemia, so that a device capable of conveniently detecting tetracycline residues in meat and fresh milk is needed.

Through retrieval, for example, patent No. CN212207115U discloses a device for detecting tetracycline residues in meat and fresh milk based on a fluorescent probe, which comprises a linear slide rail, a rack, a gear, a servo motor, a U-shaped bracket, a main cylinder assembly, an auxiliary cylinder assembly, a side plate and a flow channel, wherein the side walls of two sides of the flow channel are fixedly connected with the linear slide rail through screws, the upper end surface of the linear slide rail is provided with the rack, two ends of the U-shaped bracket are slidably connected with the linear slide rail, the inner wall of the U-shaped bracket is provided with the gear, the gear is engaged with the rack, the gear is connected with the servo motor, one side of the middle part of the U-shaped bracket is rotatably connected with the side plate through a rotating shaft, the middle part of the U-shaped bracket is provided with the main cylinder assembly, and the middle part of the side plate is provided with the auxiliary cylinder assembly. The main air cylinder component and the auxiliary air cylinder component simultaneously drive the fluorescent probe to be inserted into meat or milk along different directions, so that bidirectional detection is realized, the detection time is saved, the detection efficiency is improved, and the production cost is saved.

For example, patent No. CN212159702U discloses a device for detecting tetracycline residue in meat and fresh milk, which comprises a sealing upper cover, a processing box, a sealing plug, a scattering mechanism, a sealing washer, an extraction mechanism, a driving motor, a protective sleeve and a clamping head, wherein meat or fresh milk products are placed in the processing box, the sealing plug and the sealing upper cover are sequentially installed, the clamping head at the bottom of a motor shaft of the driving motor on the sealing upper cover is clamped and embedded in an extension rod and an embedded head, the driving motor is started to drive the motor shaft and a scattering shaft at the lower end to rotate, the scattering blade is used for scattering and uniformly mixing the meat or fresh milk products, the driving motor is closed and the sealing plug is opened, the extraction mechanism is inserted into the processing box, the detection extraction needle is contacted with the products by operating an adjusting handle, and after the sample is extracted, the detection extraction needle is detached for sample detection of liquid chromatography, wherein, the meat or the fresh milk products are mixed evenly, and the data obtained by detecting the extracted sample has higher accuracy.

Based on the above, the conventional detection device for tetracycline residues in meat and fresh milk based on the fluorescent probe is generally of a box structure, and a structure capable of fully mixing the sample and the fluorescent probe is not provided, so that the decomposed sample residues cannot be washed and sterilized after the detection is finished, the tetracycline content in the visualized sample cannot be quickly obtained, and the detection efficiency is low.

Therefore, the existing requirements are not met, and a device for detecting tetracycline residues in meat and fresh milk based on a fluorescent probe is provided.

Disclosure of Invention

Problem (A)

The invention aims to provide a device for detecting tetracycline residues in meat and fresh milk based on a fluorescent probe, which aims to solve the problems that the traditional device for detecting tetracycline residues in meat and fresh milk based on a fluorescent probe, which is proposed in the background art, is generally of a box structure, and a structure capable of fully mixing a sample and the fluorescent probe is not arranged, a decomposed sample residue cannot be washed and disinfected after detection is finished, the tetracycline content in an imaged sample cannot be obtained quickly, and the detection efficiency is low.

(II) technical scheme

In order to achieve the purpose, the invention provides the following technical scheme: a detection device for tetracycline residues in meat and fresh milk based on a fluorescent probe comprises a bearing table;

the receiving table comprises four groups of supporting columns, middle leakage holes and an insulation box, the supporting columns are fixedly connected to the bottom surface of the receiving table, the middle leakage holes vertically penetrate through the middle of the receiving table, and the insulation box is fixedly connected to the left side of the top surface of the receiving table;

a PCR fluorescence quantitative instrument is arranged on the right side of the top surface of the bearing table;

the bottom surface of the bearing table is fixedly connected with a pressure pump;

a fluorescence detection structure is arranged at the top of the bearing table;

and a raw material processing mechanism is arranged in the fluorescence detection structure.

Preferably, the fluorescence detection structure comprises:

the bottom cone barrel is fixedly connected to the top of the bearing table and is positioned above the middle leakage hole;

and the horizontal frame is fixedly connected to the top surface of the bearing table, and the inner curved side surface of the top of the horizontal frame is fixed with the outer curved side surface of the bottom cone barrel.

Preferably, the raw material processing mechanism includes:

the top fixing frame is fixedly connected to the top surface of the bottom cone barrel;

the decomposition barrel is fixedly connected to the top of the top fixing frame.

Preferably, the raw material processing mechanism further includes:

the washing pipe penetrates through the right side of the decomposition barrel;

and the bottom leakage pipe is fixedly connected between the bottom of the decomposition barrel and the top of the bottom cone barrel.

Preferably, the raw material processing mechanism further includes:

the electromagnetic valve A is arranged inside the bottom leakage pipe in a flange connection mode;

the three-way pipe is fixedly connected to the right side of the bottom cone barrel, and the right end of the three-way pipe is connected with the pressure pump;

and the electromagnetic valve B is fixedly connected to the top of the three-way pipe.

Preferably, the raw material processing mechanism further includes:

the sealing sleeve cover is arranged at the top of the decomposition barrel through sliding connection;

and the top shaft rod vertically penetrates through the middle part of the sealing sleeve cover.

Preferably, the raw material processing mechanism further includes:

the number of the decomposition wheels is three, and the decomposition wheels are coaxially connected and arranged in the top shaft rod;

and the injection pipe is fixedly connected to the top of the sealing cover.

Preferably, the fluorescence detection structure further comprises:

the sample spray head is positioned in the bottom cone barrel, and the top of the sample spray head is connected with the bottom of the bottom leakage pipe;

the inclined pressurizing pipe is fixedly connected to the right side of the sample spray head;

and the electromagnetic valve C is fixedly connected inside the right end of the inclined pressurizing pipe.

Preferably, the fluorescence detection structure further comprises:

the laser generator is fixedly connected to the inner curved side surface of the bottom cone barrel;

the inspection module is fixedly connected to the inner curved side surface of the bottom cone barrel and is positioned on the same horizontal line with the laser generator;

the protective mirror is of a convex structure and is fixedly connected to the front end inside the inspection module;

the lens is arranged on the rear side of the protective mirror;

the optical filter is arranged on the rear side of the lens;

and the fluorescence detector is arranged on the rear side of the optical filter.

Preferably, the raw material processing mechanism further includes:

the reciprocating screw rod is fixedly connected to the top surface of the bearing table and is positioned on the rear side of the bottom cone barrel;

and the variable-speed motor is fixedly connected to the front end of the reciprocating screw ball nut seat, and a rotating shaft at the bottom of the variable-speed motor is coaxial with the top shaft rod.

(III) advantageous effects

1. According to the invention, the decomposition barrel is arranged, after the fluorescent probe is injected into the injection pipe by using the injector, the sealing sleeve cover is controlled to be vertically pressed downwards by the reciprocating lead screw to seal the decomposition barrel, and the top shaft rod can drive the three groups of decomposition wheels to rotate by controlling the variable speed motor to operate, so that the sample can be conveniently crushed, and the sample and the fluorescent probe can be fully mixed.

2. The invention also arranges the flushing pipe, and the flushing pipe is matched with a plurality of groups of electromagnetic valves, after the detection is finished, the electromagnetic valve B can be opened by closing the electromagnetic valve A, and after the disinfectant liquid is added into the barrel at the bottom of the pressure pump, the operation of the pressure pump is controlled, so that the disinfectant liquid flushes and disinfects the interior of the decomposition barrel through the flushing pipe, and the decayed sample residues can be flushed and disinfected.

3. The invention also arranges a detection module, sample solution doped with the fluorescent probe is pressurized by a pressurizing pump, is sprayed out from the bottom of the spray head, emits laser beams through a laser generator to irradiate with the sample, captures images through a lens at the front end of the detection module, irradiates light images through the lens to an optical filter, filters the images through the optical filter, detects the images through a fluorescent detector, processes fluorescent data through a circuit by using a PCR fluorescent quantitative instrument, can quickly obtain the tetracycline content in the imaged sample, and can improve the detection efficiency.

Drawings

FIG. 1 is a schematic front side perspective view of an embodiment of the present invention;

FIG. 2 is an enlarged view of a portion of FIG. 1 in accordance with an embodiment of the present invention;

FIG. 3 is a schematic bottom perspective view of the embodiment of the present invention;

FIG. 4 is a schematic perspective exploded view of the disassembled barrel according to the embodiment of the present invention;

FIG. 5 is a schematic view of a disassembled structure of the bottom cone barrel according to an embodiment of the present invention;

FIG. 6 is a schematic perspective view of an exploded barrel side section in an embodiment of the present invention;

FIG. 7 is a schematic side-sectional perspective view of a detection module according to an embodiment of the present invention;

in fig. 1 to 7, the correspondence between the part names or lines and the reference numbers is:

1. a receiving table;

101. a support pillar; 102. a middle leak hole; 103. a heat preservation box;

2. a PCR fluorescence quantitative analyzer;

3. a bottom cone barrel;

301. a horizontal frame; 302. a top fixing frame; 303. a decomposition barrel; 3031. a flush tube; 3032. a bottom drain pipe; 3033. an electromagnetic valve A; 304. a three-way pipe; 3041. a solenoid valve B; 305. sealing the sleeve cover; 3051. a top shaft lever; 3052. a decomposition wheel; 3053. an injection pipe; 306. a sample spray head; 3061. an inclined pressurizing pipe; 3062. a solenoid valve C; 307. a laser generator; 308. a checking module; 3081. protective glasses; 3082. a lens; 3083. an optical filter; 3084. a fluorescence detector;

4. a pressure pump;

5. a reciprocating screw;

501. a variable speed motor.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1 to 7, an embodiment of the present invention includes: a detection device for tetracycline residues in meat and fresh milk based on a fluorescent probe comprises a bearing table 1;

the receiving table 1 comprises four groups of supporting columns 101, middle leak holes 102 and heat preservation boxes 103, the supporting columns 101 are fixedly connected to the bottom surface of the receiving table 1, the middle leak holes 102 vertically penetrate through the middle of the receiving table 1, the heat preservation boxes 103 are fixedly connected to the left side of the top surface of the receiving table 1, and fluorescent probes are stored in the heat preservation boxes 103;

a PCR fluorescence quantitative instrument 2 is arranged on the right side of the top surface of the receiving platform 1;

the bottom surface of the bearing table 1 is fixedly connected with a pressure pump 4;

a fluorescence detection structure is arranged at the top of the receiving platform 1;

the fluorescence detection structure is internally provided with a raw material processing mechanism.

As shown in fig. 7, the fluorescence detection structure further includes:

the laser generator 307 is fixedly connected with the inner curved side surface of the bottom cone barrel 3;

the inspection module 308 is fixedly connected to the inner curved side surface of the bottom cone barrel 3, and the inspection module 308 and the laser generator 307 are positioned on the same horizontal line;

a protective glass 3081 having a convex structure and fixedly connected to the front end of the interior of the inspection module 308;

a lens 3082, the lens 3082 is arranged at the rear side of the protective glass 3081;

the filter 3083, the filter 3083 is set up in the rear side of lens 3082;

the fluorescence detector 3084 and the fluorescence detector 3084 are arranged at the rear side of the optical filter 3083, and the real-time fluorescence quantitative pcr instrument consists of a fluorescence quantitative system and a computer and is used for monitoring the fluorescence in the circulating process. A computer connected to the real-time device collects the fluorescence data, the data is displayed in the form of a graph by developed real-time analysis software, the raw data is plotted as fluorescence intensity versus cycle number, and analysis can begin after the raw data is collected. The software of the real-time equipment can normalize the collected data to make up the difference of background fluorescence, a threshold level can be set after normalization, namely the level of the analyzed fluorescence data, the sample solution doped with the fluorescent probe is pressurized by the pressurizing pump 4, is sprayed out from the bottom of the spray head, emits laser beams through the laser generator 307 to irradiate with the sample, captures images through the lens 3082 at the front end of the inspection module 308, irradiates the light shadow through the lens 3082 to the optical filter 3083, performs filtering processing on the light shadow through the optical filter 3083, and performs inspection through the fluorescence detector 3084, and the PCR fluorescence quantitative instrument 2 processes the fluorescence data through a circuit, so that the tetracycline content in the imaged sample can be quickly obtained.

The fluorescence detection structure comprises:

the bottom cone barrel 3 is fixedly connected to the top of the bearing table 1 and is positioned above the middle leakage hole 102;

horizontal bracket 301, horizontal bracket 301 fixed connection are in the 1 top surface of accepting the platform, and the inside curve side in its top is fixed mutually with the outside curve side of end cone bucket 3.

The raw material processing mechanism comprises:

the top fixing frame 302, the top fixing frame 302 is fixedly connected to the 3 surface of the bottom cone barrel;

and the decomposition barrel 303 is fixedly connected to the top of the top fixing frame 302.

The raw materials processing mechanism still includes:

a flushing pipe 3031, wherein the flushing pipe 3031 is arranged on the right side of the decomposition barrel 303 in a penetrating manner;

and a bottom leakage pipe 3032 fixedly connected between the bottom of the decomposition barrel 303 and the top of the bottom cone barrel 3.

Wherein, as shown in fig. 2, the raw material processing mechanism further comprises:

the electromagnetic valve A3033 is arranged inside the bottom drain pipe 3032 through flange connection;

the three-way pipe 304 is fixedly connected to the right side of the bottom cone barrel 3, and the right end of the three-way pipe is connected with the pressure pump 4;

the electromagnetic valve B3041 and the electromagnetic valve B3041 are fixedly connected to the top of the three-way pipe 304, when the decomposition barrel 303 is cleaned, the electromagnetic valve B3041 can be opened while the electromagnetic valve A3033 is closed, and after disinfectant is added to the barrel at the bottom of the pressure pump 4, the operation of the pressure pump 4 is controlled, so that the disinfectant can flush and sterilize the inside of the decomposition barrel 303 through the flushing pipe 3031, and the decomposed sample residues can be flushed and sterilized.

The raw materials processing mechanism still includes:

a sealing cover 305, the sealing cover 305 is arranged on the top of the decomposition barrel 303 through sliding connection;

the top shaft rod 3051 is vertically arranged in the middle of the sealing sleeve cover 305 in a penetrating way, wherein the top shaft rod 3051 is arranged in the middle of the sealing sleeve cover 305 in a penetrating way.

As shown in fig. 7, the fluorescence detection structure further includes:

the sample spray head 306 is positioned inside the bottom cone barrel 3, and the top of the sample spray head is connected with the bottom of the bottom drain pipe 3032;

an inclined pressurizing pipe 3061 fixedly connected to the right side of the sample spray head 306, the left side of the inclined pressurizing pipe 3061 being inclined sixty degrees downward from the connection portion of the sample spray head 306;

the electromagnetic valve C3062 and the electromagnetic valve C3062 are fixedly connected to the inside of the right end of the inclined pressurizing pipe 3061, after a sample mixed with the fluorescent probe enters the inside of the sample nozzle 306, the sample can be rapidly atomized to facilitate fluorescent quantitative detection after the inclined direction of the inside of the sample is pressurized through the inclined pressurizing pipe 3061 by controlling the operation of the pressurizing pump 4.

Wherein, as shown in fig. 1, the raw material processing mechanism further comprises:

the reciprocating screw rod 5 is fixedly connected to the top surface of the bearing table 1 and is positioned on the rear side of the bottom cone barrel 3;

the variable speed motor 501 is fixedly connected to the front end of the ball nut seat of the reciprocating screw 5, a rotating shaft at the bottom of the variable speed motor is coaxial with the top shaft rod 3051, the sealing sleeve cover 305 can be controlled to vertically move by controlling the reciprocating screw 5 to operate, the opening and closing of the decomposition barrel 303 can be conveniently controlled, and the sealing can be conveniently and quickly carried out.

Wherein, as shown in fig. 4, the raw material processing mechanism further comprises:

the decomposition wheels 3052 are arranged in three groups, and the decomposition wheels 3052 are coaxially connected and arranged inside the top shaft 3051;

injection pipe 3053, injection pipe 3053 fixed connection is at sealed housing cap 305 top, injection pipe 3053 top fixedly connected with rubber cap, through using the syringe to inject fluorescence probe into injection pipe 3053 inside back, through reciprocal lead screw 5 control sealed housing cap 305 perpendicular pushing down carry out the sealed back to decomposition bucket 303, through control gear motor 501 operation, can make apical axis pole 3051 drive three groups decomposition wheel 3052 and rotate, be convenient for carry out the breakage to the sample, can make sample and fluorescence probe intensive mixing.

In another embodiment of the present invention, a filter screen is sleeved on the inner side of the sample nozzle 306, so that the flowing large particle meat sample can be intercepted by the filter screen, and the blocking of fluorescence detection in the fluorescence detection process can be prevented.

The working principle is as follows:

when the device is used, firstly, the device is placed on a horizontal plane, the sealing sleeve cover 305 is controlled to vertically move upwards by controlling the operation of the reciprocating screw 5, the top of the decomposition barrel 303 is opened, a sample is placed in the decomposition barrel 303, the fluorescent probe is taken out from the heat insulation box 103, the fluorescent probe is injected into the injection pipe 3053 by using the injector, the sealing sleeve cover 305 is controlled to vertically press downwards by the reciprocating screw 5 to seal the decomposition barrel 303, the top shaft 3051 can drive the three groups of decomposition wheels 3052 to rotate by controlling the operation of the variable speed motor 501, the sample and the fluorescent probe are fully mixed after the sample is crushed, the electromagnetic valve A3033 simultaneously controls the operation of the pressure pump 4, the sample is rapidly atomized after the internal oblique direction of the sample is pressurized by the oblique pressure pipe 3061, the sample solution doped with the fluorescent probe is pressurized by the pressure pump 4, after the sample is sprayed from the bottom of the spray head, laser beams are emitted by a laser generator 307 to irradiate the sample, images are captured by a lens 3082 at the front end of an inspection module 308, light and shadow are irradiated to an optical filter 3083 by the lens 3082, the light and shadow are filtered by the optical filter 3083, after the detection is performed by a fluorescence detector 3084, fluorescence data are processed by a PCR fluorescence quantitative instrument 2 through a circuit, the tetracycline content in the imaged sample can be quickly obtained, after the detection is finished, an electromagnetic valve A3033 can be closed, an electromagnetic valve B3041 can be opened at the same time, disinfectant water is added into a barrel at the bottom of a pressure pump 4, the operation of the pressure pump 4 is controlled, the disinfectant water is used for washing and disinfecting the interior of a decomposition barrel 303 through a washing pipe 3031, and the decomposed sample residues can be washed and disinfected.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a detection apparatus for tetracycline residue in meat and fresh milk based on fluorescence probe which characterized in that: comprises a bearing table (1);

the bearing table (1) comprises support columns (101), middle leak holes (102) and heat preservation boxes (103), the number of the support columns (101) is four, the support columns (101) are fixedly connected to the bottom surface of the bearing table (1), the middle leak holes (102) vertically penetrate through the middle of the bearing table (1), and the heat preservation boxes (103) are fixedly connected to the left side of the top surface of the bearing table (1);

a PCR fluorescent quantitative instrument (2) is arranged on the right side of the top surface of the bearing table (1);

the bottom surface of the bearing table (1) is fixedly connected with a pressure pump (4);

the top of the bearing table (1) is provided with a fluorescence detection structure;

2. The device for detecting tetracycline residues in meat and fresh milk based on fluorescent probe as claimed in claim 1, wherein said fluorescent detection structure comprises:

the bottom cone barrel (3), the bottom cone barrel (3) is fixedly connected to the top of the bearing platform (1) and is positioned above the middle leakage hole (102);

the horizontal frame (301), horizontal frame (301) fixed connection is in the top surface of accepting platform (1), and its top incurvate side is fixed mutually with end awl bucket (3) incurvate side.

3. The apparatus for detecting tetracycline residues in meat and fresh milk based on fluorescent probe as claimed in claim 1, wherein said material processing mechanism comprises:

the top fixing frame (302), the top fixing frame (302) is fixedly connected to the top surface of the bottom cone barrel (3);

the decomposition barrel (303), the decomposition barrel (303) is fixedly connected with the top of the top fixing frame (302).

4. The apparatus for detecting tetracycline residues in meat and fresh milk based on fluorescent probe as claimed in claim 3, wherein said material processing mechanism further comprises:

the flushing pipe (3031), the flushing pipe (3031) is arranged on the right side of the decomposition barrel (303) in a penetrating way;

and the bottom leakage pipe (3032) is fixedly connected between the bottom of the decomposition barrel (303) and the top of the bottom cone barrel (3).

5. The apparatus for detecting tetracycline residues in meat and fresh milk based on fluorescent probe as claimed in claim 4, wherein said material processing mechanism further comprises:

the electromagnetic valve A (3033) is arranged inside the bottom leakage pipe (3032) through flange connection;

the three-way pipe (304) is fixedly connected to the right side of the bottom cone barrel (3), and the right end of the three-way pipe is connected with the pressure pump (4);

and the electromagnetic valve B (3041), the electromagnetic valve B (3041) is fixedly connected with the top of the three-way pipe (304).

6. The apparatus for detecting tetracycline residues in meat and fresh milk based on fluorescent probe as claimed in claim 5, wherein said material processing mechanism further comprises:

the sealing sleeve cover (305), the sealing sleeve cover (305) is arranged at the top of the decomposition barrel (303) through sliding connection;

the top shaft lever (3051) vertically penetrates through the middle part of the sealing sleeve cover (305) and the top shaft lever (3051).

7. The apparatus for detecting tetracycline residues in meat and fresh milk based on fluorescent probe as claimed in claim 6, wherein said material processing mechanism further comprises:

the number of the decomposition wheels (3052) is three, and the decomposition wheels (3052) are coaxially connected and arranged inside the top shaft lever (3051);

and the injection pipe (3053), wherein the injection pipe (3053) is fixedly connected to the top of the sealing sleeve cover (305).

8. The device for detecting tetracycline residues in meat and fresh milk based on fluorescent probe as claimed in claim 2, wherein said fluorescent detection structure further comprises:

the sample spray head (306) is positioned inside the bottom cone barrel (3), and the top of the sample spray head is connected with the bottom of the bottom drain pipe (3032);

an inclined pressurizing pipe (3061) fixedly connected to the right side of the sample spray head (306);

and the electromagnetic valve C (3062) is fixedly connected to the inside of the right end of the inclined pressurizing pipe (3061).

9. The apparatus for detecting tetracycline residues in meat and fresh milk based on fluorescent probe as claimed in claim 8, wherein said fluorescent detection structure further comprises:

the laser generator (307), the laser generator (307) is fixedly connected with the inner curved side surface of the bottom cone barrel (3);

the inspection module (308) is fixedly connected to the inner curved side surface of the bottom cone barrel (3), and the inspection module (308) and the laser generator (307) are positioned on the same horizontal line;

a protective glass (3081) which is a convex structure and is fixedly connected with the front end inside the inspection module (308);

a lens (3082), the lens (3082) is arranged on the rear side of the protective mirror (3081);

a filter (3083), the filter (3083) is arranged at the rear side of the lens (3082);

and a fluorescence detector (3084), wherein the fluorescence detector (3084) is disposed on the rear side of the filter (3083).

10. The apparatus for detecting tetracycline residues in meat and fresh milk based on fluorescent probe as claimed in claim 7, wherein said material processing mechanism further comprises:

the reciprocating screw rod (5), the reciprocating screw rod (5) is fixedly connected to the top surface of the bearing platform (1) and is positioned on the rear side of the bottom cone barrel (3);

and the variable speed motor (501) is fixedly connected to the front end of the ball nut seat of the reciprocating screw rod (5), and the rotating shaft at the bottom of the variable speed motor is coaxial with the top shaft rod (3051).