CN114516851A - Method for extracting soybean isoflavone - Google Patents
Method for extracting soybean isoflavone Download PDFInfo
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- CN114516851A CN114516851A CN202210198778.2A CN202210198778A CN114516851A CN 114516851 A CN114516851 A CN 114516851A CN 202210198778 A CN202210198778 A CN 202210198778A CN 114516851 A CN114516851 A CN 114516851A
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- soybean
- isoflavone
- soybean isoflavone
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/34—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 3 only
- C07D311/36—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 3 only not hydrogenated in the hetero ring, e.g. isoflavones
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention belongs to the technical field of isoflavone preparation, and discloses a method for extracting soybean isoflavone, which comprises the steps of sequentially carrying out impurity screening, color sorting, drying, petal breaking, enzyme deactivation, rolling, organic solvent extraction and hot air drying treatment on soybeans to obtain defatted soybean meal; soaking defatted soybean meal, drying, pulverizing, adding ethanol, reflux extracting, mixing filtrates, and concentrating under reduced pressure to obtain a first crude extract of soybean isoflavone; adding ethyl acetate into the first crude extract of soybean isoflavone for extraction, dissolving with ethanol, mixing with silica gel, eluting, analyzing, and concentrating under reduced pressure to obtain second crude extract of soybean isoflavone; dissolving the second crude extract with acetone or ethanol, recrystallizing, mixing with the metal organic framework compound, evaporating under reduced pressure, and grinding to obtain soybean isoflavone. The method for extracting the soybean isoflavone has the advantages of simple process, low energy consumption, high separation efficiency, high yield of the soybean isoflavone, good quality and low cost, and can effectively avoid the variation of the soybean isoflavone.
Description
Technical Field
The invention belongs to the technical field of isoflavone preparation, and particularly relates to a method for extracting soybean isoflavone.
Background
At present, Soybean Isoflavone (SIF) is an active substance in soybeans, is an indispensable component in the physiological and biochemical processes of human bodies, and has multiple biological functions of preventing osteoporosis, reducing blood fat, resisting oxidation, relieving climacteric syndrome of women and the like. With the intensive research on the functionality of the soybean isoflavone, the demand of the soybean isoflavone in China also rapidly rises, and the soybean isoflavone is more and more widely applied to the industries of medicine, health care products, food, beverage and the like.
The separation and extraction method of isoflavone includes column chromatography (silica gel column chromatography, polyamide column chromatography, macroporous adsorbent resin column chromatography, etc.), membrane separation method and high speed countercurrent chromatography. The column chromatography can separate the soybean isoflavone from the sample liquid by the action of a molecular sieve and intermolecular force (hydrogen bond or Van der Waals force), and the high-purity soybean isoflavone product can be obtained by the method; the membrane separation method is based on molecular weight cut-off principle to separate soybean isoflavone from other impurities. However, the existing soybean isoflavone extraction method has the problems of complex process, high energy consumption, low separation efficiency, low yield, overhigh cost and the like. Therefore, there is a need to design a new method for extracting soy isoflavones.
Through the above analysis, the problems and defects of the prior art are as follows: the existing method for extracting the soybean isoflavone has the problems of complex process, high energy consumption, low separation efficiency, low yield, overhigh cost and the like.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for extracting soybean isoflavone.
The present invention is achieved by a method for extracting soybean isoflavone, comprising the steps of:
step one, preparing degreased bean pulp: sequentially carrying out impurity screening, color sorting, drying, petal breaking, enzyme deactivation, rolling, organic solvent extraction and hot air drying on soybeans to obtain defatted soybean meal;
step two, preparing a first crude extract of the soybean isoflavone: soaking defatted soybean meal, drying, pulverizing, adding ethanol, reflux extracting, mixing filtrates, and concentrating under reduced pressure to obtain a first crude extract of soybean isoflavone;
step three, extracting, loading and eluting the soybean isoflavone crude extract: adding ethyl acetate into the first crude extract of soybean isoflavone for extraction, dissolving with ethanol, mixing with silica gel, eluting, analyzing, and concentrating under reduced pressure to obtain a second crude extract of soybean isoflavone;
Step four, carrying out recrystallization treatment on the second crude extract of the soybean isoflavone: dissolving the obtained second crude extract of soybean isoflavone with acetone or ethanol, recrystallizing, mixing with the metal organic framework compound, evaporating under reduced pressure, and grinding to obtain soybean isoflavone.
Further, the preparation method of the defatted soybean meal in the first step comprises the following steps:
(1) respectively removing impurities from the soybean raw material, screening and carrying out color selection treatment;
(2) respectively drying, breaking and inactivating enzyme on the bean grains subjected to color sorting treatment to obtain bean paste;
(3) and (3) sequentially carrying out rolling, organic solvent extraction and hot air drying treatment on the broad beans to obtain the defatted soybean meal.
Further, after the bean grains are dried, the moisture of the bean grains is adjusted to 5-12%.
Further, the enzyme deactivation treatment method comprises the following steps: and (3) putting the broad beans in a steam enzyme deactivation treatment at 85-95 ℃ for 10-20 min.
Further, the preparation method of the soybean isoflavone crude extract in the second step comprises the following steps:
(1) selecting prepared defatted soybean meal as a raw material, adding deionized water which is 5-10 times of the weight of the defatted soybean meal into the selected defatted soybean meal, soaking for 3-5 hours, drying for 2-4 hours at 75-80 ℃, and crushing;
(2) Adding 6-10 times of ethanol with the concentration of 70-80% into the defatted soybean meal, reflux-extracting for 3 times, filtering, and combining the 3 times of extraction filtrates to obtain a soybean isoflavone filtrate;
(3) vacuum concentrating the soybean isoflavone filtrate under reduced pressure, and recovering ethanol until the concentrated solution has no ethanol smell to obtain a first crude extract of soybean isoflavone.
Further, the temperature of the reduced pressure concentration is 45-55 ℃, and the pressure is 0.1-0.5 Pa.
Further, the extraction, sample loading and elution treatment of the first crude extract of soybean isoflavone in the third step comprises the following steps:
(1) adding ethyl acetate into the first crude extract of the soybean isoflavone for extraction to obtain an ethyl acetate phase; vacuum concentrating ethyl acetate phase under reduced pressure to obtain soybean isoflavone extract;
(2) dissolving the obtained soybean isoflavone extract with ethanol, mixing with silica gel, stirring for dispersing, drying, and adding into chromatographic column filled with silica gel;
(3) eluting the chromatographic column with eluent, detecting and analyzing under ultraviolet lamp, mixing and collecting the parts with the same ultraviolet absorption at 254nm, and concentrating under reduced pressure to obtain a second crude extract of soybean isoflavone.
Further, the volume ratio of the first crude extract of the soybean isoflavone to the ethyl acetate is 1: 2-3: 5.
Further, the recrystallization treatment of the second crude extract of soybean isoflavones in the fourth step comprises:
(1) dissolving the obtained second crude extract of soybean isoflavone with acetone or ethanol, and recrystallizing to obtain soybean isoflavone crystal;
(2) mixing the soy isoflavone crystals with the metal organic framework compound to obtain a metal organic framework compound adsorbed with soy isoflavones;
(3) evaporating the obtained metal organic framework compound with adsorbed soybean isoflavone under reduced pressure, evaporating to remove water, and grinding to obtain soybean isoflavone.
Further, the temperature of the reduced pressure evaporation is less than or equal to 80 ℃.
By combining all the technical schemes, the invention has the advantages and positive effects that: the method for extracting the soybean isoflavone has the advantages of simple process, low energy consumption, high separation efficiency, high yield of the soybean isoflavone, good quality and low cost, and can effectively avoid the variation of the soybean isoflavone.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of a method for extracting soy isoflavones according to embodiments of the present invention.
Fig. 2 is a flowchart of a method for preparing defatted soybean meal according to an embodiment of the present invention.
FIG. 3 is a flow chart of the preparation method of crude extract of soybean isoflavone according to the embodiment of the present invention.
FIG. 4 is a flow chart of the process of the first crude extract of soy isoflavones for extraction, loading and elution according to the present invention.
FIG. 5 is a flow chart of the method for recrystallization of the second crude extract of soy isoflavones according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In view of the problems of the prior art, the present invention provides a method for extracting soy isoflavones, which is described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the method for extracting soy isoflavone according to the embodiment of the present invention comprises the following steps:
s101, preparing defatted soybean meal: sequentially carrying out impurity screening, color sorting, drying, petal breaking, enzyme deactivation, rolling, organic solvent extraction and hot air drying on soybeans to obtain defatted soybean meal;
S102, preparing a first crude extract of the soybean isoflavone: soaking defatted soybean meal, drying, pulverizing, adding ethanol, reflux extracting, mixing filtrates, and concentrating under reduced pressure to obtain a first crude extract of soybean isoflavone;
s103, extracting, loading and eluting the soybean isoflavone crude extract: adding ethyl acetate into the first crude extract of soybean isoflavone for extraction, dissolving with ethanol, mixing with silica gel, eluting, analyzing, and concentrating under reduced pressure to obtain a second crude extract of soybean isoflavone;
s104, carrying out recrystallization treatment on the second crude extract of the soybean isoflavone: dissolving the obtained second crude extract of soybean isoflavone with acetone or ethanol, recrystallizing, mixing with the metal organic framework compound, evaporating under reduced pressure, and grinding to obtain soybean isoflavone.
As shown in fig. 2, the method for preparing the defatted soybean meal in step S101 according to the embodiment of the present invention includes:
s201, respectively removing impurities from the soybean raw material, screening and carrying out color sorting treatment;
s202, drying, breaking and inactivating enzyme of the bean grains subjected to color selection treatment to obtain bean paste;
s203, sequentially rolling, extracting with an organic solvent and drying with hot air to obtain the defatted soybean meal.
After the bean grains are dried, the moisture of the bean grains is adjusted to 5-12%.
The enzyme deactivation treatment method provided by the embodiment of the invention comprises the following steps: and (3) putting the broad beans in a steam enzyme deactivation treatment at 85-95 ℃ for 10-20 min.
As shown in fig. 3, the preparation method of the crude extract of soy isoflavone in step S102 provided by the embodiment of the present invention comprises:
s301, selecting the prepared defatted soybean meal as a raw material, adding deionized water which is 5-10 times of the weight of the defatted soybean meal into the selected defatted soybean meal, soaking for 3-5 hours, drying for 2-4 hours at 75-80 ℃, and crushing;
s302, adding 6-10 times of ethanol with the concentration of 70-80% by weight into the defatted soybean meal, performing reflux extraction for 3 times, filtering, and combining the 3 times of extraction filtrate to obtain a soybean isoflavone filtrate;
s303, concentrating the soybean isoflavone filtrate under vacuum and reduced pressure, and recovering ethanol until the concentrated solution has no ethanol smell to obtain a first crude extract of soybean isoflavone.
The reduced pressure concentration temperature provided by the embodiment of the invention is 45-55 ℃, and the pressure is 0.1-0.5 Pa.
As shown in fig. 4, the extraction, loading and elution processes of the first crude extract of soy isoflavone in step S103 provided by the embodiment of the present invention include:
S401, adding ethyl acetate into the first crude extract of the soybean isoflavone for extraction to obtain an ethyl acetate phase; vacuum concentrating ethyl acetate phase under reduced pressure to obtain soybean isoflavone extract;
s402, dissolving the obtained soybean isoflavone extract with ethanol, mixing with silica gel, stirring for uniform dispersion, drying, and adding into a chromatographic column filled with silica gel;
and S403, eluting the chromatographic column with the eluent, detecting and analyzing under an ultraviolet lamp, combining and collecting the parts with the same ultraviolet absorption of 254nm, and concentrating under reduced pressure to obtain a second crude extract of the soybean isoflavone.
The volume ratio of the first crude extract of the soybean isoflavone to the ethyl acetate provided by the embodiment of the invention is 1: 2-3: 5.
as shown in fig. 5, the recrystallization treatment of the second crude extract of soy isoflavones in step S104 provided by the embodiments of the present invention includes:
s501, dissolving the obtained second crude extract of the soybean isoflavone with acetone or ethanol, and recrystallizing to obtain a soybean isoflavone crystal;
s502, mixing the soybean isoflavone crystals with the metal-organic framework compound to obtain the metal-organic framework compound adsorbed with the soybean isoflavone;
s503, evaporating the obtained metal organic framework compound adsorbed with the soybean isoflavone under reduced pressure, evaporating to remove water in the metal organic framework compound, and grinding to obtain the soybean isoflavone.
The temperature of the reduced pressure evaporation provided by the embodiment of the invention is less than or equal to 80 ℃.
The above description is only for the purpose of illustrating the embodiments of the present invention, and the scope of the present invention should not be limited thereto, and any modifications, equivalents and improvements made by those skilled in the art within the technical scope of the present invention as disclosed in the present invention should be covered by the scope of the present invention.
Claims (10)
1. A method for extracting soybean isoflavone is characterized by comprising the following steps:
step one, preparing degreased bean pulp: sequentially carrying out impurity screening, color sorting, drying, petal breaking, enzyme deactivation, rolling, organic solvent extraction and hot air drying treatment on soybeans to obtain defatted soybean meal;
step two, preparing a first crude extract of the soybean isoflavone: soaking defatted soybean meal, drying, pulverizing, adding ethanol, reflux extracting, mixing filtrates, and concentrating under reduced pressure to obtain a first crude extract of soybean isoflavone;
step three, extracting, loading and eluting the soybean isoflavone crude extract: adding ethyl acetate into the first crude extract of soybean isoflavone for extraction, dissolving with ethanol, mixing with silica gel, eluting, analyzing, and concentrating under reduced pressure to obtain a second crude extract of soybean isoflavone;
Step four, carrying out recrystallization treatment on the second crude extract of the soybean isoflavone: dissolving the second crude extract with acetone or ethanol, recrystallizing, mixing with the metal organic framework compound, evaporating under reduced pressure, and grinding to obtain soybean isoflavone.
2. The method for extracting soybean isoflavones as claimed in claim 1, wherein the method for preparing the defatted soybean meal in the first step comprises:
(1) respectively removing impurities from the soybean raw material, screening and carrying out color selection treatment;
(2) respectively drying, breaking and inactivating enzymes of the bean grains subjected to color sorting treatment to obtain bean paste;
(3) and (3) sequentially carrying out rolling, organic solvent extraction and hot air drying treatment on the broad beans to obtain defatted soybean meal.
3. The method for extracting soybean isoflavone according to claim 2, wherein the moisture content of the soybean grains is adjusted to 5 to 12% after the soybean grains are dried.
4. The method for extracting soybean isoflavone as claimed in claim 2, wherein the enzyme deactivation treatment comprises: and (3) putting the broad beans in a steam enzyme deactivation treatment at 85-95 ℃ for 10-20 min.
5. The method for extracting soy isoflavones as claimed in claim 1, wherein the crude soy isoflavone extract of step two is prepared by a process comprising:
(1) Selecting the prepared defatted soybean meal as a raw material, adding deionized water which is 5-10 times of the weight of the defatted soybean meal into the selected defatted soybean meal, soaking for 3-5 hours, drying for 2-4 hours at 75-80 ℃, and crushing;
(2) adding 6-10 times of ethanol with the concentration of 70-80% into the defatted soybean meal, reflux-extracting for 3 times, filtering, and combining the 3 times of extraction filtrates to obtain a soybean isoflavone filtrate;
(3) vacuum concentrating the soybean isoflavone filtrate under reduced pressure, and recovering ethanol until the concentrated solution has no ethanol smell to obtain first crude extract of soybean isoflavone.
6. The method for extracting soybean isoflavones as claimed in claim 5, wherein the concentration under reduced pressure is carried out at a temperature of 45 to 55 ℃ under a pressure of 0.1 to 0.5 Pa.
7. The method for extracting soybean isoflavone according to claim 1, wherein the extraction, sample loading and elution treatment of the first crude extract of soybean isoflavone in the third step comprises:
(1) adding ethyl acetate into the first crude extract of the soybean isoflavone for extraction to obtain an ethyl acetate phase; vacuum concentrating ethyl acetate phase under reduced pressure to obtain soybean isoflavone extract;
(2) dissolving the obtained soybean isoflavone extract with ethanol, mixing with silica gel, stirring, dispersing, drying, and adding into chromatography column filled with silica gel;
(3) Eluting the chromatographic column with eluent, detecting and analyzing under an ultraviolet lamp, mixing and collecting the parts with the same ultraviolet absorption at 254nm, and concentrating under reduced pressure to obtain a second crude extract of the soybean isoflavone.
8. The method for extracting soy isoflavones of claim 7 wherein the volume ratio of the first crude soy isoflavone extract to ethyl acetate is from 1: 2-3: 5.
9. the method for extracting soy isoflavones as claimed in claim 1 wherein the recrystallization of the second crude soy isoflavone extract of step four comprises:
(1) dissolving the obtained second crude extract of soybean isoflavone with acetone or ethanol, and recrystallizing to obtain soybean isoflavone crystal;
(2) mixing the soy isoflavone crystals with the metal-organic framework compound to obtain a metal-organic framework compound adsorbed with soy isoflavone;
(3) evaporating the obtained metal organic framework compound with adsorbed soybean isoflavone under reduced pressure, evaporating to remove water, and grinding to obtain soybean isoflavone.
10. The method for extracting soy isoflavones of claim 9 wherein the reduced pressure evaporation is at a temperature of 80℃ or less.
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| CN202210198778.2A CN114516851A (en) | 2022-03-01 | 2022-03-01 | Method for extracting soybean isoflavone |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116114790A (en) * | 2022-11-21 | 2023-05-16 | 翁源县新南都饲料科技有限公司 | Fermented feed for enzymolysis of tea and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109160910A (en) * | 2018-08-23 | 2019-01-08 | 天津纳尔生物科技有限公司 | A kind of daizeol extraction extraction process |
| CN111875571A (en) * | 2020-07-23 | 2020-11-03 | 长沙理工大学 | Method for extracting soybean isoflavone from soy sauce residues |
| CN112869020A (en) * | 2021-03-05 | 2021-06-01 | 克东禹王大豆蛋白食品有限公司 | Production device of low-temperature defatted soybean meal with low beany flavor and preparation method thereof |
-
2022
- 2022-03-01 CN CN202210198778.2A patent/CN114516851A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109160910A (en) * | 2018-08-23 | 2019-01-08 | 天津纳尔生物科技有限公司 | A kind of daizeol extraction extraction process |
| CN111875571A (en) * | 2020-07-23 | 2020-11-03 | 长沙理工大学 | Method for extracting soybean isoflavone from soy sauce residues |
| CN112869020A (en) * | 2021-03-05 | 2021-06-01 | 克东禹王大豆蛋白食品有限公司 | Production device of low-temperature defatted soybean meal with low beany flavor and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 高荣海, 等: "大豆异黄酮制备工艺的研究", 《农业科技与装备》, no. 02, pages 69 - 71 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116114790A (en) * | 2022-11-21 | 2023-05-16 | 翁源县新南都饲料科技有限公司 | Fermented feed for enzymolysis of tea and preparation method thereof |
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