CN114504328B

CN114504328B - Apparatus and method of operation for studying brain activity in an animal during waking to anaesthesia

Info

Publication number: CN114504328B
Application number: CN202111653609.5A
Authority: CN
Inventors: 刘勇; 卢呈祥
Original assignee: Guangzhou First Peoples Hospital
Current assignee: Guangzhou First Peoples Hospital
Priority date: 2021-12-30
Filing date: 2021-12-30
Publication date: 2023-03-07
Anticipated expiration: 2041-12-30
Also published as: CN114504328A

Abstract

The invention discloses equipment and an operation method for researching brain activity of an animal in the processes from waking to anaesthesia, and the equipment comprises an anaesthesia mechanism, a recording mechanism, a moving mechanism and a fixing mechanism, wherein a box cover of the anaesthesia mechanism is connected with a base to form a sealed cavity, the base is provided with an air inlet nozzle, an air outlet nozzle and a data interface which are used for providing anaesthesia gas, a heating component is arranged in the sealed cavity, the recording mechanism comprises an LDE lamp group, an infrared camera, an LED curing lamp and a brain imager, the moving mechanism comprises a lifting seat and a running disc which is rotatably arranged, the fixing mechanism comprises a lifting column and a head ring, the lifting end of the lifting column is detachably connected with the head ring, and the head ring is arranged on the top of the animal cranium through photosensitive adhesive and the LED curing lamp. The equipment and the operation method for researching the brain activity of the animal from waking to anaesthetizing can improve the air pressure control precision and the stability of micro-droplet generation.

Description

Apparatus and method of operation for studying brain activity in an animal during waking to anaesthesia

Technical Field

The invention relates to the technical field of life science research, in particular to a device and an operation method for researching brain activities of animals from waking to anaesthesia.

Background

In the study of living animals, the dynamic observation of the brain activity of living animals by a two-photon microscope is a common technical means. At present, the two-photon microscope is used as a research basis for the fixed and dynamic behavior observation of living animals, and the number of the devices is very small.

In the traditional research technical means, the general anesthesia research is mainly used, the small animal is locally fixed by utilizing the existing simple fixing device after the general anesthesia operation, and the research range is limited. In addition, when the existing equipment is used for living body imaging, the observed part of an animal is generally fixed by dental cement, and the body of the animal cannot move freely on a platform. The research mode has the advantages that the damage to animals is large, the expansion of a research range is limited, the animals easily struggle in a waking state, and the vibration of an observed fixed part is caused, so that an imaging focal plane is unstable. Therefore, two-photon in vivo imaging experiments in a conscious state or semi-conscious state of a living animal cannot be done using existing devices.

Disclosure of Invention

The purpose of the invention is: the equipment and the operation method for researching the brain activity of the animal from waking to anaesthesia are provided, the imaging effect can be improved, the brain activity research of the living animal under different states can be realized, the harm to the animal is reduced, and the application range of the research can be expanded.

In order to achieve the above object, according to an embodiment of the first aspect of the present invention, there is provided an apparatus for studying brain activities of an animal from waking to anaesthesia, including an anaesthesia mechanism, a recording mechanism, a moving mechanism and a fixing mechanism, wherein the anaesthesia mechanism includes a box cover, a base and a heating assembly; the box cover and the base are connected to form a sealed cavity, and the base is provided with an air inlet nozzle and an air outlet nozzle which are communicated with the sealed cavity; the heating assembly is arranged in the sealing cavity; the base is provided with a data interface for communicating the sealed cavity with the outside; the recording mechanism comprises an LDE lamp group, an infrared camera, an LED curing lamp and a brain imager which are arranged in the sealed cavity; the LED lamp set, the infrared camera and the LED curing lamp are electrically connected with the data interface; the LED lamp bank and the infrared camera are arranged in the sealed cavity; the brain imager is arranged on the box cover; the movable mechanism is arranged in the sealed cavity and comprises a lifting seat and a running disc, the bottom end of the lifting seat is fixedly connected with the base, and the lifting end of the lifting seat is rotatably connected with the running disc through a bearing; the fixing mechanism is arranged in the sealing cavity and comprises a lifting column and a head ring, the bottom end of the lifting column is fixedly connected with the base, and the lifting end of the lifting column is detachably connected with the head ring; the head ring is arranged in a camera area of the brain imager.

According to some embodiments of the invention, the cover is made of an acrylic material.

According to some embodiments of the invention, the anesthesia mechanism further comprises a temperature sensor for adjusting the temperature of the heating assembly.

According to some embodiments of the invention, the temperature sensor is configured to detect a temperature of the capsule.

According to some embodiments of the invention, the temperature sensor is for detecting an animal body temperature.

According to some embodiments of the invention, the lid and the base are connected by a snap-fit fastening.

The present invention also provides a method of operating an apparatus for studying brain activity during waking to anaesthesia in an animal as defined in the first aspect, comprising the steps of:

s1, mounting the head ring on the animal skull top through photosensitive adhesive and an LED curing lamp;

s2, mounting a head ring connected with the animal skull on a lifting column, and placing the animal body on a running disc;

s3, connecting the base and the box cover; the air inlet pipe is connected with the air inlet nozzle, and the air outlet pipe is connected with the air outlet nozzle; the data interface is accessed to an external controller;

s4, turning on the brain imager, the infrared camera and the LED lamp set;

s5, recording data of the brain imager and data of the infrared camera when the animal is in a waking state;

and S6, introducing anesthetic gas into the gas inlet pipe, starting the heating assembly to enable the animal to enter an anesthetic state from a waking state, and recording data of the brain imager and data of the infrared camera in the process. .

According to some embodiments of the invention, further comprising the steps of: in step S6, the heating temperature of the heating assembly is adjusted so that the animal is at different temperatures, and the data of the brain imager and the data of the infrared camera are recorded in the above process, so as to study the brain activities of the animal at different temperature states.

According to some embodiments of the invention, further comprising the steps of: in step S2, the height of the lifting column is adjusted according to the body size of the animal.

According to some embodiments of the invention, further comprising the steps of: in step S2, the distance between the running plate and the box cover is adjusted through the lifting seat according to the body type of the animal.

Compared with the prior art, the equipment and the operation method for researching the brain activity of the animal from waking to anaesthesia have the beneficial effects that:

according to the device and the operation method for researching the brain activity of the animal in the processes from waking to anaesthesia, the head ring, the photosensitive adhesive and the LED curing lamp are matched to fix the head of the animal, so that the damage to the animal can be reduced, the application range of research can be expanded, the anxiety of the animal can be reduced, the struggling strength of the animal can be reduced, and the imaging effect can be improved; the running disc can be freely and rotatably arranged through the bearing, four limbs of an animal are placed on the running disc, and the running disc can rotate along with the animal when the animal moves, so that the anxiety of the animal is powerfully reduced, the struggling strength of the animal is reduced, the imaging effect is favorably improved, the imaging operation of the animal in a waking state is realized, a driven animal body fixing mode is replaced, and the brain activity research of the freely moving animal is realized; the anesthesia device and the recording device are arranged, and the animal is anesthetized according to the requirement, so that the study on the brain activities of the animal in a waking state, a state from waking to anesthesia and a general anesthesia state is realized. Therefore, the equipment and the operation method for researching the brain activity of the animal from waking to anaesthesia provided by the embodiment of the invention can improve the imaging effect, realize the brain activity research of the living animal in different states, reduce the damage to the animal and be beneficial to expanding the application range of the research.

Drawings

FIG. 1 is a schematic diagram of the switch mechanism of the apparatus for studying brain activity during waking to anaesthesia in an animal according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of a first configuration of an anesthesia apparatus for use with an apparatus for studying brain activity during waking to anesthesia in an animal in accordance with an embodiment of the present invention;

FIG. 3 is a schematic diagram of a second configuration of an anesthesia apparatus of an apparatus for studying brain activity during waking to anesthesia in an animal in accordance with an embodiment of the present invention;

fig. 4 is a flow chart of steps of a method of operation of an apparatus for studying brain activity during waking to anaesthesia in an animal in accordance with an embodiment of the present invention.

Reference numerals are as follows:

a box cover 11; a base 12; an air intake nozzle 121; an air outlet nozzle 122; a data interface 123; a heating assembly 13;

an LDE lamp group 21; an infrared camera 22; a brain imager 23;

a lifting base 31; a running plate 32;

a lifting column 41; a head ring 42.

Detailed Description

The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is the orientation or positional relationship described based on the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, a plurality of means is one or more, a plurality of means is two or more, and greater than, less than, more than, etc. are understood as excluding the essential numbers, and greater than, less than, etc. are understood as including the essential numbers. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.

An apparatus and method of operation for studying brain activity during waking to anaesthesia in an animal according to an embodiment of the invention will now be described with reference to figures 1 to 4.

As shown in fig. 1 to 3, the apparatus for studying brain activity of an animal from waking to anaesthesia according to the first aspect of the present invention comprises an anaesthesia mechanism, a recording mechanism, a moving mechanism and a fixed mechanism.

As shown in fig. 1 to 3, the anesthesia apparatus comprises a lid 11, a base 12 and a heating unit 13. The box cover 11 is of a box-like structure, one plane of the box is an opening, the base 12 is a square bottom plate, the shape of the base 12 is matched with that of the box opening, the box cover 11 covers the base 12 to realize sealing connection with the base 12, and a sealing cavity is formed between the base 12 and the box cover 11. It will be appreciated that the lid 11 and base 12 are connected by snap fastening, thereby preventing the lid 11 from being removed from the base 12 during the study. It should be noted that the box cover 11 can be made of transparent materials such as acrylic and glass, so as to facilitate observation of the state of the animal in the sealed cavity.

The base 12 is provided with an air inlet nozzle 121 and an air outlet nozzle 122 which are communicated with the sealing cavity, the air inlet nozzle 121 and the air outlet nozzle 122 are connected with the box cover 11 in a sealing manner and are arranged at the outer end of the sealing cavity, the air inlet nozzle 121 is connected with an air inlet pipe, and the air outlet nozzle 122 is connected with an air outlet pipe.

The heating assembly 13 is fixedly mounted on the chassis and arranged in the sealed cavity for keeping the temperature of the sealed cavity, thereby being beneficial to keeping the temperature of the animal in an anesthesia state. It should be noted that, the heating assembly 13 may be electrically connected to a plurality of temperature sensors, and the temperature sensors may be used to detect the temperature of the sealed cavity or the body temperature of the animal, so as to adjust the parameters of the heating assembly 13 according to the detected data of the temperature sensors and the body temperature value requirement of the animal.

The base 12 is provided with a data interface 123 for communicating the sealed cavity with the outside, the data interface 123 is connected with the box cover 11 in a sealing manner and leaks outside the box cover 11, so that the connection between the electric element in the sealed cavity and the outside element is realized through the data interface 123.

The recording mechanism comprises an LDE lamp group 21, an infrared camera 22, an LED curing lamp and a brain imager 23 which are arranged in the sealed cavity. The LED lamp set, the infrared camera 22 and the LED curing lamp are electrically connected with the data interface 123, so that connection with an external controller is realized. The LED lamp bank and the infrared camera 22 are arranged in the sealed cavity, the movement behavior of the animal is recorded through the infrared camera 22, and the illumination brightness in the sealed cavity is adjusted through the LED lamp bank. It can be understood that the LED light set can be composed of a plurality of light bars, for example, four light bars, which are respectively disposed at four corners of the base 12.

The brain imager 23 is arranged on the box cover 11, the brain imager 23 penetrates through the box cover 11, one end of the brain imager 23 protrudes out of the upper surface of the box cover 11 and is electrically connected with an external controller, and the camera end of the brain imager 23 is arranged below the box cover 11 and is arranged in the sealed cavity. The brain imager 23 may be configured as a two-photon microscope, a two-photon fluorescence microscope, a single-photon fluorescence microscope, or a far-infrared molding device.

Moving mechanism sets up in sealed intracavity, including lift seat 31 and race dish 32, the bottom of lift seat 31 and the center fixed connection of base 12, the lift end of lift seat 31 passes through the bearing and runs the coaxial rotation of dish 32 and be connected, thereby can be according to the size of animal, through the height of adjusting lift seat 31 lift end, distance between adjustable race dish 32 and the lid 11, improve the comfort level of animal in the research process, reduce the anxiety of animal, reduce the vibrations that the animal struggled to arouse, improve the formation of image effect.

The fixing mechanism is disposed in the sealing chamber and includes a lifting column 41 and a head ring 42, a bottom end of the lifting column 41 is fixedly connected to a side end of the base 12, and the lifting column 41 is disposed outside the running plate 32. The lift end of lift post 41 is connected with the one end of horizontal plate, and the other end and the head ring 42 of horizontal plate can be dismantled and be connected to can be according to the size of animal, through the height of adjusting lift post 41, adjustable head ring 42 and the distance of running between the dish 32 improve the comfort level of animal in the research process, reduce the anxiety of animal, reduce the vibrations that the animal struggled and arouse, improve the formation of image effect. It should be noted that the head ring 42 can be made of metal material, and further, the head ring 42 can be detachably connected to the lifting column 41 by screws.

The headband 42 is positioned in the imaging area of the brain imager 23 such that when the animal's cranium is mounted on the headband 42, the animal's cranium is within the imaging area of the brain imager 23, thereby recording the animal's brain activity via the brain imager 23.

As shown in fig. 4, the operation method of the apparatus for studying brain activity during waking to anaesthesia in an animal according to the second aspect of the invention comprises the following steps:

the head loop 42 is mounted on the animal vertex by photosensitive adhesive and LED curing lamps, and it should be noted that in the mounting process, attention needs to be paid to parallelism between the head loop 42 and the vertex, so that the vertex is uniformly connected with the head loop 42. The head ring 42 connected with the animal skull is arranged on the lifting column 41, the animal body is placed on the running disc 32, the base 12 is connected with the box cover 11, the inlet pipe is connected into the air inlet nozzle 121, the outlet pipe is connected into the air outlet nozzle 122, and the data interface 123 is connected into the external controller.

And (3) turning on the brain imager 23, the infrared camera 22, the LED lamp set and the heating assembly 13 to enable the animal to be in a waking state, and recording relevant data of the brain imager 23 and the infrared camera 22 for researching the brain activity of the animal in the waking state.

And introducing anesthetic gas into the air inlet pipe to enable the animal to enter an anesthetic state from a waking state, and recording relevant data of the brain imager 23 and the infrared camera 22 in the process so as to be used for researching brain activity of the animal in the process of entering the anesthetic state from the waking state and researching brain activity of the animal in the anesthetic state.

It can be understood that the temperature of the sealed cavity is adjusted by the heating assembly 13 during and after the anesthesia, which is beneficial to maintaining the normal body temperature of the animal under the anesthesia state, so that the experimental data is more prepared. Furthermore, the temperature of the sealed cavity is controlled by the heating component 13, so that the animal is at different temperatures, and the related data of the brain imager 23 and the infrared camera 22 are recorded under different temperature states of the movable animal, thereby realizing the research of the animal under various states.

According to the device and the operation method for researching the brain activity of the animal from waking to anaesthesia, the head ring 42, the photosensitive adhesive and the LED curing lamp are matched to fix the head of the animal, so that the damage to the animal can be reduced, the application range of research can be expanded, the anxiety of the animal can be reduced, the struggling strength of the animal can be reduced, and the imaging effect can be improved; the running disc 32 can be freely and rotatably arranged through a bearing, the limbs of an animal are placed on the running disc 32, and the running disc 32 can rotate along with the animal when the animal moves, so that the anxiety of the animal is powerfully reduced, the struggling strength of the animal is reduced, the imaging effect is favorably improved, the imaging operation of the animal in an awake state is realized, a driven animal body fixing mode is replaced, and the brain activity research of the freely moving animal is realized; the anesthesia device and the recording device are arranged, and the animal is anesthetized according to the requirement, so that the brain activity research of the animal in a waking state, a waking to anesthesia state and a general anesthesia state is realized. Therefore, the equipment and the operation method for researching the brain activity of the animal from waking to anaesthesia provided by the embodiment of the invention can improve the imaging effect, realize the brain activity research of the living animal in different states, reduce the damage to the animal and be beneficial to expanding the application range of the research.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples" or the like mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

While embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that: numerous changes, modifications, substitutions and variations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. Apparatus for studying brain activity during waking to anaesthesia in an animal, comprising:

the anesthesia mechanism comprises a box cover, a base and a heating component; the box cover and the base are connected to form a sealed cavity, and the base is provided with an air inlet nozzle and an air outlet nozzle which are communicated with the sealed cavity; the heating assembly is arranged in the sealing cavity; the base is provided with a data interface for communicating the sealed cavity with the outside;

the recording mechanism comprises an LED lamp group, an infrared camera, an LED curing lamp and a brain imager which are arranged in the sealed cavity; the LED lamp group, the infrared camera and the LED curing lamp are electrically connected with the data interface; the LED lamp bank and the infrared camera are arranged in the sealed cavity; the brain imager is arranged on the box cover;

the movable mechanism is arranged in the sealed cavity and comprises a lifting seat and a running disc, the bottom end of the lifting seat is fixedly connected with the base, and the lifting end of the lifting seat is rotatably connected with the running disc through a bearing;

the fixing mechanism is arranged in the sealing cavity and comprises a lifting column and a head ring, the bottom end of the lifting column is fixedly connected with the base, and the lifting end of the lifting column is detachably connected with the head ring; the head ring is arranged in a camera shooting area of the brain imager and is arranged on the top of an animal skull through photosensitive adhesive.

2. The apparatus of claim 1, wherein the cover is made of acrylic material.

3. The apparatus for studying brain activity during wakefulness to anesthesia of an animal according to claim 1, wherein said anesthesia mechanism further comprises a temperature sensor for regulating the temperature of said heating assembly.

4. The apparatus for studying brain activity during wakefulness to anesthesia of an animal according to claim 3, wherein said temperature sensor is adapted to sense the temperature of said sealed chamber.

5. The apparatus for studying brain activity during wakefulness to anesthesia of an animal according to claim 3, wherein said temperature sensor is adapted to sense a body temperature of the animal.

6. The apparatus for studying brain activity during wakefulness to anaesthesia in an animal according to claim 1, wherein the lid and the base are fastened together by a snap fit.