CN114486831A - Cat progesterone fluorescence quantitative determination reagent - Google Patents
Cat progesterone fluorescence quantitative determination reagent Download PDFInfo
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- CN114486831A CN114486831A CN202210083827.8A CN202210083827A CN114486831A CN 114486831 A CN114486831 A CN 114486831A CN 202210083827 A CN202210083827 A CN 202210083827A CN 114486831 A CN114486831 A CN 114486831A
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- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 title claims abstract description 68
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 62
- 229960003387 progesterone Drugs 0.000 title claims abstract description 34
- 239000000186 progesterone Substances 0.000 title claims abstract description 34
- 239000000523 sample Substances 0.000 claims abstract description 70
- 241000282326 Felis catus Species 0.000 claims abstract description 32
- 239000012528 membrane Substances 0.000 claims abstract description 32
- 238000001514 detection method Methods 0.000 claims abstract description 30
- 239000003085 diluting agent Substances 0.000 claims abstract description 28
- 239000004005 microsphere Substances 0.000 claims abstract description 22
- 241000282324 Felis Species 0.000 claims abstract description 21
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 20
- 230000010100 anticoagulation Effects 0.000 claims abstract description 17
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000000427 antigen Substances 0.000 claims abstract description 7
- 102000036639 antigens Human genes 0.000 claims abstract description 7
- 108091007433 antigens Proteins 0.000 claims abstract description 7
- 239000012488 sample solution Substances 0.000 claims abstract description 7
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims abstract description 5
- 102000004506 Blood Proteins Human genes 0.000 claims abstract description 5
- 108010017384 Blood Proteins Proteins 0.000 claims abstract description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 5
- 229920002884 Laureth 4 Polymers 0.000 claims abstract description 5
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims abstract description 5
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000008103 glucose Substances 0.000 claims abstract description 5
- 229960000789 guanidine hydrochloride Drugs 0.000 claims abstract description 5
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims abstract description 5
- 235000011090 malic acid Nutrition 0.000 claims abstract description 5
- 239000001630 malic acid Substances 0.000 claims abstract description 5
- 239000008213 purified water Substances 0.000 claims abstract description 5
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 235000019333 sodium laurylsulphate Nutrition 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 150000001875 compounds Chemical class 0.000 claims description 21
- 239000011259 mixed solution Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 8
- 239000012470 diluted sample Substances 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 238000004587 chromatography analysis Methods 0.000 claims description 6
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 4
- 229910052782 aluminium Inorganic materials 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 claims description 4
- 239000008280 blood Substances 0.000 claims description 4
- 239000011888 foil Substances 0.000 claims description 4
- 238000005259 measurement Methods 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- 229960001484 edetic acid Drugs 0.000 abstract description 16
- 230000013011 mating Effects 0.000 abstract description 6
- 230000012173 estrus Effects 0.000 abstract description 3
- 239000007850 fluorescent dye Substances 0.000 abstract description 3
- 238000001215 fluorescent labelling Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004246 corpus luteum Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000004696 endometrium Anatomy 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000002611 ovarian Effects 0.000 description 1
- 239000000583 progesterone congener Substances 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention provides a fluorescence quantitative detection reagent for feline progesterone, and relates to the technical field of feline progesterone detection. The fluorescence quantitative detection reagent for the feline progesterone comprises a reagent card, sample diluent, an EDTA (ethylene diamine tetraacetic acid) anticoagulation tube and a pipette tip; the reagent card comprises a reagent card body, and a PVC shell, a combination pad and an NC membrane which are arranged on the reagent card body, wherein fluorescent microspheres for fluorescent labeling are arranged in the combination pad, and T lines and C lines are arranged on the surface of the NC membrane; the sample diluent consists of the following raw materials in parts by mass: 10 to 25 percent of glucose, 3 to 7 percent of fatty acid-free serum protein, 0.2 to 3 percent of sodium lauryl sulfate, 0.1 to 1 percent of sodium azide, 0.1 to 0.5 percent of lauromacrogol, 0.15 to 0.18 percent of malic acid, 0.1 to 1 percent of guanidine hydrochloride and the balance of purified water. The concentration of the antigen in the sample solution can be obtained according to the intensity of fluorescence, the concentration of progesterone in cat plasma can be accurately detected, the living physical state of the cat can be rapidly and accurately known, and the kit has guiding significance for subsequent mating and oestrus mating of the cat.
Description
Technical Field
The invention relates to the technical field of feline progesterone detection, and particularly relates to a feline progesterone fluorescence quantitative detection reagent.
Background
Progesterone is a natural progestogen secreted by the ovarian corpus luteum, and has a significant morphological effect on estrogen-stimulated endometrium in vivo, necessary for maintenance. The rising or the reduction of progesterone in the cat body can directly know whether the cat is oestrous or not, and interference of different stages can be timely performed on the cat.
The traditional cat progesterone detection process is complicated, and the cat progesterone detection cannot be flexibly realized, so that the life state of a cat cannot be mastered in time.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a fluorescence quantitative detection reagent for feline progesterone, which solves the problem that the traditional feline progesterone detection is not flexible enough.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a fluorescence quantitative detection reagent for feline progesterone comprises a reagent card, sample diluent, an EDTA anticoagulation tube and a pipette tip;
the reagent card comprises a reagent card body and a PVC shell, a combination pad and an NC membrane which are arranged on the reagent card body, wherein fluorescent microspheres for fluorescent marks are arranged in the combination pad, a T line and a C line are arranged on the surface of the NC membrane, one end of the combination pad is fixedly connected with one end of the T line of the NC membrane, a sample adding hole is formed in the PVC shell, and the sample adding hole is formed in one side of the combination pad;
the fluorescence-labeled fluorescent microspheres are used for reacting with the progesterone level in the plasma of the cat;
the sample diluent consists of the following raw materials in parts by mass: 10 to 25 percent of glucose, 3 to 7 percent of fatty acid-free serum protein, 0.2 to 3 percent of sodium lauryl sulfate, 0.1 to 1 percent of sodium azide, 0.1 to 0.5 percent of lauromacrogol, 0.15 to 0.18 percent of malic acid, 0.1 to 1 percent of guanidine hydrochloride and the balance of purified water.
Preferably, the pipette tip is used for sucking and dropping a mixture of a cat plasma sample and a sample diluent on the bonding pad in the sample adding hole, and the EDTA anticoagulation tube is used for collecting cat plasma.
Preferably, the detection method of the feline progesterone fluorescence quantitative detection reagent comprises the following steps:
s1, taking a plasma sample of a cat to be detected, adding cat plasma into a sample diluent, uniformly mixing, sucking and dripping a mixed solution after uniform mixing into a sample adding hole by using a pipette tip, and after the mixed solution is dripped onto a combination pad, diffusing fp4 in the sample on the combination pad and combining with fluorescent microspheres to form a compound;
s2, the compound diffuses from the binding pad to one end of a T line of the NC membrane along with the sample diluent, the compound and fP4 coated on the T line compete to bind the labeled antibody, the more fP4 in the sample is, the less labeled antibody captured on the T line is, the weaker the fluorescence signal of the T line is, the non-captured fluorescent microsphere compound and the labeled antibody continue to diffuse to the C line along the NC membrane and are captured by the antibody coated on the C line, and the C line is formed;
s3, taking the bonding pad and the NC membrane out of the PVC shell, putting the bonding pad and the NC membrane into an XQ-1000 fluorescence detector, detecting by using the XQ-1000 fluorescence detector, detecting the reagent strip after chromatography is finished, and obtaining the concentration of the antigen in the sample solution according to the intensity of fluorescence.
Preferably, the use method of the fluorescence quantitative detection reagent for the feline progesterone comprises the following steps:
step one, turning on an instrument switch, and logging in a test system;
step two, taking out 1 reagent card, 1 tube of diluent and 1 EDTA anticoagulation tube from the kit;
step three, centrifuging the collected EDTA anticoagulation blood at 3000-;
tearing the aluminum foil bag open, taking out the reagent card, taking 60-80 mu L of the diluted sample by using a pipette, and dropwise adding the diluted sample into a sample adding hole of the reagent card;
step five, inserting the reagent card into the card slot of the instrument after the sample adding is finished, and automatically detecting after the instrument is incubated for 10-12 minutes;
and step six, after the measurement is finished, the instrument can automatically display the measured concentration value and print the detection result.
(III) advantageous effects
The invention provides a fluorescence quantitative detection reagent for feline progesterone. The method has the following beneficial effects:
the invention uses the pipette suction head to suck and drop the mixed liquid into the sample adding hole, after the mixed liquid is dropped on the combination pad, fp4 in the sample is diffused on the combination pad and combined with the fluorescent microsphere to form a compound, meanwhile, the complex and fP4 coated on the T line compete for binding the labeled antibody, the more fP4 in the sample, the less labeled antibody captured on the T line, the weaker the fluorescence signal of the T line, the uncaptured fluorescent microsphere complex and the labeled antibody continue to diffuse to the C line along the NC membrane and are captured by the antibody coated on the C line to form the C line, simultaneously, an XQ-1000 fluorescence detector is used for detecting the reagent strip after the chromatography is finished, the concentration of the antigen in the sample solution can be obtained according to the intensity of the fluorescence, the concentration of the progesterone in the plasma of the cat can be accurately detected, simultaneously can be fast accurate know the cat living physical state, have guiding significance to cat follow-up mating and estrus mating.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
the embodiment of the invention provides a fluorescence quantitative detection reagent for feline progesterone, which comprises a reagent card, sample diluent, an EDTA (ethylene diamine tetraacetic acid) anticoagulation tube and a pipette tip; the reagent card comprises a reagent card body and a PVC shell, a combination pad and an NC membrane which are arranged on the reagent card body, wherein fluorescent microspheres for fluorescent labeling are arranged in the combination pad, a T line and a C line are arranged on the surface of the NC membrane, one end of the combination pad is fixedly connected with one end of the T line of the NC membrane, a sample adding hole is formed in the PVC shell, and the sample adding hole is formed in one side of the combination pad; the fluorescence-labeled fluorescent microspheres are used for reacting with the progesterone level in the cat plasma; the sample diluent consists of the following raw materials in parts by mass: 10% -25% of glucose, 3% of fatty acid-free serum protein, 0.2% of sodium lauryl sulfate, 0.1% of sodium azide, 0.1% of lauromacrogol, 0.15% of malic acid, 0.1% of guanidine hydrochloride and the balance of purified water, wherein the mixed solution is dripped into a sample hole by sucking with a pipette tip, after the mixed solution is dripped on a combination pad, fP4 in a sample diffuses on the combination pad and combines with fluorescent microspheres to form a compound, the compound and fP4 coated on a T line compete to combine with a labeled antibody, the more fP4 in the sample, the less labeled antibody captured on the T line, the weaker fluorescent signal of the T line, the fluorescent microsphere compound and the labeled antibody which are not captured continue to diffuse to the C line along an NC membrane, the fluorescent microsphere compound and the labeled antibody are captured by the antibody coated on the C line to form the C line, and the reagent strip after the completion of the chromatography is detected by an XQ-1000 fluorescence detector, the concentration of the antigen in the sample solution can be obtained according to the intensity of fluorescence, the concentration of progesterone in cat plasma can be accurately detected, the living physical state of the cat can be rapidly and accurately known, and the kit has guiding significance for subsequent mating and oestrus mating of the cat.
The pipette tip is used for sucking and dropping a mixture of a cat plasma sample and a sample diluent on the bonding pad in the sample adding hole, and the EDTA anticoagulation tube is used for collecting the cat plasma.
The detection method of the fluorescence quantitative detection reagent for the feline progesterone comprises the following steps:
s1, taking a plasma sample of a cat to be detected, adding cat plasma into a sample diluent, uniformly mixing, sucking and dripping a mixed solution after uniform mixing into a sample adding hole by using a pipette tip, and after the mixed solution is dripped onto a combination pad, diffusing fp4 in the sample on the combination pad and combining with fluorescent microspheres to form a compound;
s2, the compound diffuses from the binding pad to one end of a T line of the NC membrane along with the sample diluent, the compound and fP4 coated on the T line compete to bind the labeled antibody, the more fP4 in the sample is, the less labeled antibody captured on the T line is, the weaker the fluorescence signal of the T line is, the non-captured fluorescent microsphere compound and the labeled antibody continue to diffuse to the C line along the NC membrane and are captured by the antibody coated on the C line, and the C line is formed;
s3, taking the bonding pad and the NC membrane out of the PVC shell, putting the bonding pad and the NC membrane into an XQ-1000 fluorescence detector, detecting by using the XQ-1000 fluorescence detector, detecting the reagent strip after chromatography is finished, and obtaining the concentration of the antigen in the sample solution according to the intensity of fluorescence.
The application method of the fluorescence quantitative detection reagent for the feline progesterone comprises the following steps:
step one, turning on an instrument switch, and logging in a test system;
step two, taking out 1 reagent card, 1 tube of diluent and 1 EDTA anticoagulation tube from the kit;
step three, centrifuging the collected EDTA anticoagulation blood for 4min at 3000rpm at room temperature, taking 50 mu L of upper plasma by using a pipette, adding the upper plasma into the sample diluent, and repeatedly reversing for several times to uniformly mix;
tearing the aluminum foil bag open, taking out the reagent card, taking 60 mu L of the diluted sample by using a pipette,
dropwise adding the mixture into a sample adding hole of a reagent card;
step five, inserting the reagent card into the card slot of the instrument after the sample adding is finished, and automatically detecting after the instrument is incubated for 10 minutes;
and step six, after the measurement is finished, the instrument can automatically display the measured concentration value and print the detection result.
Example two:
the embodiment of the invention provides a fluorescence quantitative detection reagent for feline progesterone, which comprises a reagent card, sample diluent, an EDTA (ethylene diamine tetraacetic acid) anticoagulation tube and a pipette tip;
the reagent card comprises a reagent card body and a PVC shell, a combination pad and an NC membrane which are arranged on the reagent card body, wherein fluorescent microspheres for fluorescent labeling are arranged in the combination pad, a T line and a C line are arranged on the surface of the NC membrane, one end of the combination pad is fixedly connected with one end of the T line of the NC membrane, a sample adding hole is formed in the PVC shell, and the sample adding hole is formed in one side of the combination pad;
the fluorescence-labeled fluorescent microspheres are used for reacting with the progesterone level in the cat plasma;
the sample diluent consists of the following raw materials in parts by mass: 10 percent of glucose, 3 percent of fatty acid-free serum protein, 0.2 percent of sodium lauryl sulfate, 0.1 percent of sodium azide, 0.1 percent of lauromacrogol, 0.15 percent of malic acid, 0.1 percent of guanidine hydrochloride and the balance of purified water.
The pipette tip is used for sucking and dropping a mixture of a cat plasma sample and a sample diluent on the bonding pad in the sample adding hole, and the EDTA anticoagulation tube is used for collecting the cat plasma.
The detection method of the fluorescence quantitative detection reagent for the feline progesterone comprises the following steps:
s1, taking a plasma sample of a cat to be detected, adding cat plasma into a sample diluent, uniformly mixing, sucking and dripping a mixed solution after uniform mixing into a sample adding hole by using a pipette tip, and after the mixed solution is dripped onto a combination pad, diffusing fp4 in the sample on the combination pad and combining with fluorescent microspheres to form a compound;
s2, the compound diffuses from the binding pad to one end of a T line of the NC membrane along with the sample diluent, the compound and fP4 coated on the T line compete to bind the labeled antibody, the more fP4 in the sample is, the less labeled antibody captured on the T line is, the weaker the fluorescence signal of the T line is, the non-captured fluorescent microsphere compound and the labeled antibody continue to diffuse to the C line along the NC membrane and are captured by the antibody coated on the C line, and the C line is formed;
s3, taking the bonding pad and the NC membrane out of the PVC shell, putting the bonding pad and the NC membrane into an XQ-1000 fluorescence detector, detecting by using the XQ-1000 fluorescence detector, detecting the reagent strip after chromatography is finished, and obtaining the concentration of the antigen in the sample solution according to the intensity of fluorescence.
The application method of the fluorescence quantitative detection reagent for the feline progesterone comprises the following steps:
step one, turning on an instrument switch, and logging in a test system;
step two, taking out 1 reagent card, 1 tube of diluent and 1 EDTA anticoagulation tube from the kit;
step three, centrifuging the collected EDTA anticoagulation blood for 4min at 3000rpm at room temperature, taking 50 mu L of upper plasma by using a pipettor, adding the upper plasma into the sample diluent, and repeatedly reversing for several times to uniformly mix;
tearing the aluminum foil bag, taking out the reagent card, taking 60 mu L of the diluted sample by using a liquid transfer machine, and dropwise adding the diluted sample into a sample adding hole of the reagent card;
step five, inserting the reagent card into the card slot of the instrument after the sample adding is finished, and automatically detecting after the instrument is incubated for 10 minutes;
and step six, after the measurement is finished, the instrument can automatically display the measured concentration value and print the detection result.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (4)
1. A fluorescence quantitative detection reagent for feline progesterone, which is characterized in that: comprises a reagent card, sample diluent, an EDTA anticoagulation tube and a pipette tip;
the reagent card comprises a reagent card body and a PVC shell, a combination pad and an NC membrane which are arranged on the reagent card body, wherein fluorescent microspheres for fluorescent marks are arranged in the combination pad, a T line and a C line are arranged on the surface of the NC membrane, one end of the combination pad is fixedly connected with one end of the T line of the NC membrane, a sample adding hole is formed in the PVC shell, and the sample adding hole is formed in one side of the combination pad;
the fluorescence-labeled fluorescent microspheres are used for reacting with the progesterone level in the plasma of the cat;
the sample diluent consists of the following raw materials in parts by mass: 10 to 25 percent of glucose, 3 to 7 percent of fatty acid-free serum protein, 0.2 to 3 percent of sodium lauryl sulfate, 0.1 to 1 percent of sodium azide, 0.1 to 0.5 percent of lauromacrogol, 0.15 to 0.18 percent of malic acid, 0.1 to 1 percent of guanidine hydrochloride and the balance of purified water.
2. The fluorescence quantitative detection reagent for feline progesterone according to claim 1, wherein: the pipette tip is used for sucking and dropping a mixture of a cat plasma sample and a sample diluent on a bonding pad in a sample adding hole, and the EDTA anticoagulation tube is used for collecting cat plasma.
3. The method for detecting the feline progesterone fluorogenic quantitative detection reagent according to any one of claims 1-3, wherein the kit comprises: the method comprises the following steps:
s1, taking a plasma sample of a cat to be detected, adding cat plasma into a sample diluent, uniformly mixing, sucking and dripping a mixed solution after uniform mixing into a sample adding hole by using a pipette tip, and after the mixed solution is dripped onto a combination pad, diffusing fp4 in the sample on the combination pad and combining with fluorescent microspheres to form a compound;
s2, the compound diffuses from the binding pad to one end of a T line of the NC membrane along with the sample diluent, the compound and fP4 coated on the T line compete to bind the labeled antibody, the more fP4 in the sample is, the less labeled antibody captured on the T line is, the weaker the fluorescence signal of the T line is, the non-captured fluorescent microsphere compound and the labeled antibody continue to diffuse to the C line along the NC membrane and are captured by the antibody coated on the C line, and the C line is formed;
s3, taking the bonding pad and the NC membrane out of the PVC shell, putting the bonding pad and the NC membrane into an XQ-1000 fluorescence detector, detecting by using the XQ-1000 fluorescence detector, detecting the reagent strip after chromatography is finished, and obtaining the concentration of the antigen in the sample solution according to the intensity of fluorescence.
4. The use of the fluorescence quantitative detection reagent for feline progesterone according to any one of claims 1-4 wherein: the method comprises the following steps:
step one, turning on an instrument switch, and logging in a test system;
step two, taking out 1 reagent card, 1 tube of diluent and 1 EDTA anticoagulation tube from the kit;
step three, centrifuging the collected EDTA anticoagulation blood at 3000-;
tearing the aluminum foil bag open, taking out the reagent card, taking 60-80 mu L of the diluted sample by using a pipette, and dropwise adding the diluted sample into a sample adding hole of the reagent card;
step five, inserting the reagent card into the card slot of the instrument after the sample adding is finished, and automatically detecting after the instrument is incubated for 10-12 minutes;
and step six, after the measurement is finished, the instrument can automatically display the measured concentration value and print the detection result.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210083827.8A CN114486831A (en) | 2022-01-22 | 2022-01-22 | Cat progesterone fluorescence quantitative determination reagent |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210083827.8A CN114486831A (en) | 2022-01-22 | 2022-01-22 | Cat progesterone fluorescence quantitative determination reagent |
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| CN114486831A true CN114486831A (en) | 2022-05-13 |
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| CN202210083827.8A Pending CN114486831A (en) | 2022-01-22 | 2022-01-22 | Cat progesterone fluorescence quantitative determination reagent |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN207301082U (en) * | 2017-07-24 | 2018-05-01 | 吉林工程技术师范学院 | A kind of progesterone fluorescent micro-ball immune chromatography quantitative test paper bar |
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| CN113777300A (en) * | 2021-08-31 | 2021-12-10 | 浙江求致生物科技有限公司 | Detection kit for quantitatively detecting new coronavirus antibody in human blood and detection method thereof |
| CN113933522A (en) * | 2021-11-01 | 2022-01-14 | 山东鑫诺生物工程有限公司 | Cat thyroid stimulating hormone fluorescent quantitative detection reagent and preparation method thereof |
-
2022
- 2022-01-22 CN CN202210083827.8A patent/CN114486831A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207301082U (en) * | 2017-07-24 | 2018-05-01 | 吉林工程技术师范学院 | A kind of progesterone fluorescent micro-ball immune chromatography quantitative test paper bar |
| CN112485420A (en) * | 2020-11-19 | 2021-03-12 | 海卫特(广州)医疗科技有限公司 | Fluorescence immunochromatography kit for detecting feline pancreatitis and preparation method thereof |
| CN113777300A (en) * | 2021-08-31 | 2021-12-10 | 浙江求致生物科技有限公司 | Detection kit for quantitatively detecting new coronavirus antibody in human blood and detection method thereof |
| CN113933522A (en) * | 2021-11-01 | 2022-01-14 | 山东鑫诺生物工程有限公司 | Cat thyroid stimulating hormone fluorescent quantitative detection reagent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 方京冲, 姚连生: "血浆孕酮固相酶免疫荧光测定法", 检验医学, no. 03, 30 September 1991 (1991-09-30), pages 143 - 145 * |
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