CN101226152A - Automatic analysis method and liquid stabilising agent for blood serum total ferro combining ability - Google Patents
Automatic analysis method and liquid stabilising agent for blood serum total ferro combining ability Download PDFInfo
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- CN101226152A CN101226152A CNA2007100008234A CN200710000823A CN101226152A CN 101226152 A CN101226152 A CN 101226152A CN A2007100008234 A CNA2007100008234 A CN A2007100008234A CN 200710000823 A CN200710000823 A CN 200710000823A CN 101226152 A CN101226152 A CN 101226152A
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- binding capacity
- total iron
- iron binding
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Abstract
The invention relates to an automatic analysis method of serum total iron binding capacity and a relative liquid stabilizing agent, with wide application in medical and biochemistry technical field. The invention is characterized in that in alkali buffer solution, transferring is saturated by free iron ions, when the pH value of the system changes to acid, the iron ions combined with the transferring is released from the transferring to process sensitive color reaction with chromazurine B, and the system light adsorption increases while the increases is in direct proportion with the total iron binding capacity in the sample, and the invention uses an automatic biochemistry analyzer to test the total iron binding capacity content of sample via two-point end point method. The inventive liquid test agent has high stability, easy preparation, strong anti-interference ability and non pollution on the pipe of automatic biochemistry analyzer, which is suitable for automatically checking serum total iron binding capacity in batch.
Description
Technical field
The present invention relates to the autoanalyzer method and the liquid stabilising agent thereof of total iron binding capacity, can be widely used in medical science and technological field of biochemistry.
Background technology
The mensuration of total iron binding capacity (TIBC) is the routine inspection project.Numerous clinical datas show that diseases such as hypoferric anemia, oxyhepatitis can cause total iron binding capacity to reduce, and diseases such as cirrhosis, ephrosis, uremia and hemochromatosis disease can cause total iron binding capacity to increase.Measure the content of serum T IBC, the observation of the making a definite diagnosis of relevant disease, result of treatment is had important clinic value.
At present, clinical assays serum T IBC mainly adopts atomic absorption method and the various complexing photometry through light magnesium carbonate absorption.Atomic absorption method is the recommend method of mensuration TIBC, but this method not only needs special-purpose expensive instrument, and needs precipitation, centrifugal, detects the cost height, and operation steps is numerous, and clinical practice is difficult to popularize; Though easy relatively with complexing spectrphotometric method for measuring TIBC operation, this method need precipitate equally, centrifugal, can't be applied to automatic biochemical analyzer.
The objective of the invention is in order to solve the deficiency of above-mentioned technology, by transferrins at adsorbable ferric ion under the alkali condition, under acid condition, can discharge the utilization of ferric ion character, to clinically provide a kind of and need not to precipitate, centrifugal, the free of contamination automatic assay technology of pipeline and the corresponding liquid stable reagent thereof that can directly apply to automatic clinical chemistry analyzer and automatic biochemical is analyzed.This mensuration reagent is put 2-8 ℃ of preservation and can be stablized 6 months at least, and method that provides and reagent can be applied to present widely used clinical biochemical automatic analyzer, thereby reach the requirement of extensive mensuration sample.
Summary of the invention
The invention provides a kind of autoanalyzer method and liquid stabilising agent of total iron binding capacity, be used for the mensuration of total iron binding capacity.This mensuration system mainly is by in alkaline solution, transferrins is saturated by the free iron ion, when the pH value of the system of mensuration is converted to acidity, the ferric ion that combines with transferrins discharges from transferrins and with chromazurol B sensitive chromogenic reaction takes place, the absorbance of measuring system this moment rises, the rising value of its absorbance is directly proportional with total iron binding capacity content in the sample, and the measurement pattern with 2 end-point methods on automatic biochemistry analyzer realizes total iron binding capacity Determination on content in the sample.
In order to reach desirable detection effect and enough stability, 2 end-point methods provided by the invention change iron-binding capacity robotization detectable, can be divided into two components of A, B especially.The concrete composition of two components is formed and can be expressed as follows.
The A component:
PH8.4 damping fluid 50-150mmol/L
Chromazurol B 120-180 μ mol/L
Sodium bicarbonate 50-300mmol/L
Surfactant 5-30ml/L
Remove agent interfering 5-15mmol/L
Reductive agent 0.5-5g/L
Antiseptic 0.1-2g/L
The B component:
PH3.5 damping fluid 50-200mmol/L
Surfactant 5-30ml/L
The iron agent 10-50g/L that dissociates
For accuracy and the stability that improves said determination reagent, eliminate measurement result and be subjected to extraneous multiple interference, described two select end-point method measures that damping fluid can be GOOD ' S series damping fluid, glycine buffer, Tris-HCl damping fluid, citric acid-trisodium citrate damping fluid, Potassium Hydrogen Phthalate-hydrochloride buffer, HAc-NaAc damping fluid in the reagent; Surfactant can be wherein a kind of of non-ionics such as SDS, Tween20, Tween80, Brij35, Brij98, polyoxyethylene nonylphenol ether, TritonX-100; Removing agent interfering can be thiocarbamide, thiosemicarbazides; Reductive agent can be oxammonium hydrochloride, ascorbic acid; Antiseptic can be NaN
3, PC series antiseptic; The iron agent of dissociating can be urea, guanidine hydrochloride, isocyanide guanidine hydrochloride.
Embodiment
Embodiment: 2 end-point methods of serum transferrin are measured reagent, the preferred especially Tris-HCl damping fluid of damping fluid in the A component, the preferred especially acetic acid-sodium-acetate buffer of damping fluid in the B component; Particularly preferred surfactant is TritonX-100 in the A component, and particularly preferred surfactant is Tween80 in the B component; Go agent interfering to be preferably thiocarbamide; Reductive agent is preferably oxammonium hydrochloride; Antiseptic is preferably NaN
3The iron agent of dissociating is preferably guanidine hydrochloride.In mentioned reagent is formed, Tween80, TritonX-100 can make the mensuration system keep limpid in measuring process, avoid causing owing to the muddiness of protein the unusual rising of measurement system absorbance, thiocarbamide is used to eliminate the interference of serum copper, and oxammonium hydrochloride is used for Fe
3+Be reduced to Fe
2+, NaN
3Be used to suppress microbial growth, guanidine hydrochloride is used for ferric ion is come from the serum transferrin cracking.
The A component:
PH8.4 Tris-HCl damping fluid 80mmol/L
Chromazurol B 160 μ mol/L
Sodium bicarbonate 120mmol/L
TritonX-100 10ml/L
Thiocarbamide 10mmol/L
Oxammonium hydrochloride 2g/L
Antiseptic 0.6g/L
The B component:
PH3.5HAc-NaAc damping fluid 150mmol/L
Tween80 15ml/L
Guanidine hydrochloride 30g/L
When the reagent of embodiment is used to measure sample, the assay method that adopts is 2 end-point methods, temperature is 37 ℃, R1: sample: R2 is 200: 15: 50, mensuration master/commplementary wave length is 600/800nm, R1 adds after sample or the standard measuring and reads the 1st absorbance after temperature is hatched 300 seconds, adds R2 then and continues to hatch and read the 2nd absorbance after 300 seconds.
20 routine total iron binding capacity samples have been measured simultaneously with the magnesium carbonate absorption method that this law and " clinical examination working specification " (second edition) are recommended.Table 1 is the present embodiment measured value and the magnesium carbonate determination of adsorption method value table of comparisons, and the correlation coefficient r of present embodiment and atomic absorption method is 0.9973, and both have shown fabulous correlativity.
Table 1
| Ketoamine oxidase method measured value (μ mol/L) | Embodiment measured value (μ mol/L) |
| 65.9 | 65.7 |
| 54.5 | 54.8 |
| 43.7 | 42.9 |
| 56.1 | 55.2 |
| 65.4 | 65.4 |
| 78.8 | 78.5 |
| 65.3 | 66.9 |
| 78.9 | 77.6 |
| 63.8 | 63.4 |
| 57.8 | 57.0 |
| 56.4 | 56.8 |
| 77.5 | 76.9 |
| 48.1 | 49.7 |
| 56.3 | 57.1 |
| 54.5 | 54.9 |
| 44.2 | 45.6 |
| 78.6 | 77.3 |
| 67.9 | 67.5 |
| 71.6 | 70.8 |
| 73.7 | 74.5 |
The compound method of the foregoing description reagent only is used to illustrate principle of the present invention and its application, but the present invention never is confined to the above-mentioned range of application that exemplifies.
Claims (7)
1. the autoanalyzer method of a total iron binding capacity and reagent, its feature is in alkaline buffer, transferrins is saturated by the free iron ion, when the pH value of the system of mensuration is converted to acidity, the ferric ion that combines with transferrins discharges from transferrins and with chromazurol B sensitive chromogenic reaction takes place, the absorbance of measuring system this moment rises, the rising value of its absorbance is directly proportional with total iron binding capacity content in the sample, and the measurement pattern with 2 end-point methods on automatic biochemistry analyzer realizes total iron binding capacity Determination on content in the sample.
2. the autoanalyzer method of total iron binding capacity according to claim 1 and reagent is characterized in that: the mensuration system is damping fluid, chromazurol B, sodium bicarbonate, surfactant, go the dissociate combination of agent of agent interfering, reductive agent, antiseptic, iron.
3. the autoanalyzer method of total iron binding capacity according to claim 2 and reagent, it is characterized in that: described mensuration system reagent ratio is
The A component:
PH8.4 damping fluid 50-150mmol/L
Chromazurol B 120-180 μ mol/L
Sodium bicarbonate 50-300mmol/L
Surfactant 5-30ml/L
Remove agent interfering 5-15mmol/L
Reductive agent 0.5-5g/L
Antiseptic 0.1-2g/L
The B component:
PH3.5 damping fluid 50-200mmol/L
Surfactant 5-30ml/L
The iron agent 10-50g/L that dissociates
4. the autoanalyzer method of total iron binding capacity according to claim 3 and reagent, it is characterized in that: preferred surfactants is that preferred surfactants is Tween80 in TritonX-100, the B component in the A component.
5. the autoanalyzer method of total iron binding capacity according to claim 3 and reagent is characterized in that: go agent interfering to be preferably thiocarbamide.
6. the autoanalyzer method of total iron binding capacity according to claim 3 and reagent, it is characterized in that: reductive agent is preferably oxammonium hydrochloride.
7. the autoanalyzer method of total iron binding capacity according to claim 3 and reagent is characterized in that: the iron agent of dissociating is preferably guanidine hydrochloride.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100008234A CN101226152A (en) | 2007-01-16 | 2007-01-16 | Automatic analysis method and liquid stabilising agent for blood serum total ferro combining ability |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100008234A CN101226152A (en) | 2007-01-16 | 2007-01-16 | Automatic analysis method and liquid stabilising agent for blood serum total ferro combining ability |
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| CN101226152A true CN101226152A (en) | 2008-07-23 |
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|---|---|---|---|
| CNA2007100008234A Pending CN101226152A (en) | 2007-01-16 | 2007-01-16 | Automatic analysis method and liquid stabilising agent for blood serum total ferro combining ability |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323430A (en) * | 2011-08-15 | 2012-01-18 | 北京利德曼生化股份有限公司 | Stable unsaturated iron bonding force determination kit |
| CN104483494A (en) * | 2014-12-22 | 2015-04-01 | 宁波美康生物科技股份有限公司 | Serum UIBC (unsaturated iron bonding capacity) detection kit |
| CN110568206A (en) * | 2019-09-12 | 2019-12-13 | 苏州普瑞斯生物科技有限公司 | total iron binding force detection kit and preparation method thereof |
| CN111007023A (en) * | 2019-12-11 | 2020-04-14 | 天津中成佳益生物科技有限公司 | Serum total iron binding force detection kit and preparation method and detection method thereof |
| CN112714870A (en) * | 2018-11-08 | 2021-04-27 | 深圳迈瑞生物医疗电子股份有限公司 | Method and kit for detecting iron content in blood sample |
| CN113655035A (en) * | 2021-08-12 | 2021-11-16 | 深圳上泰生物工程有限公司 | Separation method, detection method and kit of sugar-deficient transferrin |
-
2007
- 2007-01-16 CN CNA2007100008234A patent/CN101226152A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323430A (en) * | 2011-08-15 | 2012-01-18 | 北京利德曼生化股份有限公司 | Stable unsaturated iron bonding force determination kit |
| CN104483494A (en) * | 2014-12-22 | 2015-04-01 | 宁波美康生物科技股份有限公司 | Serum UIBC (unsaturated iron bonding capacity) detection kit |
| CN104483494B (en) * | 2014-12-22 | 2016-09-28 | 宁波美康生物科技股份有限公司 | A kind of serum unsaturated iron-binding capacity detection kit |
| CN112714870A (en) * | 2018-11-08 | 2021-04-27 | 深圳迈瑞生物医疗电子股份有限公司 | Method and kit for detecting iron content in blood sample |
| CN110568206A (en) * | 2019-09-12 | 2019-12-13 | 苏州普瑞斯生物科技有限公司 | total iron binding force detection kit and preparation method thereof |
| CN111007023A (en) * | 2019-12-11 | 2020-04-14 | 天津中成佳益生物科技有限公司 | Serum total iron binding force detection kit and preparation method and detection method thereof |
| CN113655035A (en) * | 2021-08-12 | 2021-11-16 | 深圳上泰生物工程有限公司 | Separation method, detection method and kit of sugar-deficient transferrin |
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Open date: 20080723 |