CN114480073B - Automatic reverse quick DNA extraction element of mixing - Google Patents

Automatic reverse quick DNA extraction element of mixing Download PDF

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Publication number
CN114480073B
CN114480073B CN202210223047.9A CN202210223047A CN114480073B CN 114480073 B CN114480073 B CN 114480073B CN 202210223047 A CN202210223047 A CN 202210223047A CN 114480073 B CN114480073 B CN 114480073B
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block
turnover
shaking
test tube
shell
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CN114480073A (en
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孙雪娜
孙选侠
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Yiming Suzhou Cell Biotechnology Co ltd
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Yiming Suzhou Cell Biotechnology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65GTRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
    • B65G47/00Article or material-handling devices associated with conveyors; Methods employing such devices
    • B65G47/74Feeding, transfer, or discharging devices of particular kinds or types
    • B65G47/90Devices for picking-up and depositing articles or materials
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65GTRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
    • B65G47/00Article or material-handling devices associated with conveyors; Methods employing such devices
    • B65G47/74Feeding, transfer, or discharging devices of particular kinds or types
    • B65G47/90Devices for picking-up and depositing articles or materials
    • B65G47/905Control arrangements

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Abstract

The invention discloses a rapid DNA extraction device capable of automatically reversing and uniformly mixing, which relates to the technical field of molecular biology and comprises a shell, wherein a turnover mechanism is arranged in the shell, a shaking mechanism is arranged on the turnover mechanism, a transportation mechanism is arranged on the shell and used for clamping a test tube and conveying the test tube to the shaking mechanism, the shaking mechanism clamps the test tube, the test tube is fully shaken uniformly by the cooperation of the shaking mechanism and the turnover mechanism, the test tube is put back to the original position by the transportation mechanism after the work is finished, the problem that the experimental result is unstable due to different operation force of manual operation is avoided, and the operation time of workers and a large amount of labor force are greatly saved.

Description

Automatic reverse quick DNA extraction element of mixing
Technical Field
The invention relates to the technical field of molecular biology, in particular to a rapid DNA extraction device capable of automatically reversing and uniformly mixing.
Background
Extracting DNA is an important step for carrying out gene structure and function research, the length of an obtained fragment is generally required to be not less than 100-200kb, various factors for breaking and degrading the DNA are avoided as much as possible in the DNA extraction process so as to ensure the integrity of the DNA, the laboratory generally adopts manual mixing at present, a test tube is clamped manually, each step of reaction needs to be reversed and mixed for at least 10 minutes, time and labor are wasted for a large number of experiments, the operation strength of different experimenters is different, the experimental result is unstable, the DNA extraction separation amount is not enough or the DNA is broken easily caused, the subsequent experimental result is influenced, and along with the continuous improvement of the quality requirement, the rapid DNA extraction device capable of automatically reversing and mixing becomes the industrial requirement.
Chinese utility model patent with publication number CN 2082975U discloses a DNA extraction element, comprising a base plate, the spout has been seted up at the top of bottom plate, swing joint has first gyro wheel and second gyro wheel on the spout, the outer edge of first gyro wheel is connected along the transmission with the outer edge of second gyro wheel through the belt, belt activity suit is on the outer edge of first gyro wheel and second gyro wheel, the middle part fixed mounting on belt top has the base block, and the top fixed mounting of base block has a fixed box, and movable sleeve is equipped with closing device in the fixed box, and the quantity of first gyro wheel and second gyro wheel is two, this DNA extraction element, drives the transfer line through driving motor and rotates for the drive wheel drives the connecting strip and uses the transfer line to do circular motion as the centre of a circle, and the connecting rod of being convenient for drive first gyro wheel and second gyro wheel are reciprocating motion, are favorable to evenly oscillating to the DNA centrifuging tube, and labour saving and time saving avoids different experimenters operating dynamics different, makes the experimental result unstable. But the device can't realize automatic realization automatically clamped test tube, automatic upset mixing, transportation automatically clamped, the test tube after shaking the even dismantlement.
Disclosure of Invention
The invention discloses a rapid DNA extraction device capable of automatically reversing and uniformly mixing, which comprises a shell, wherein a turnover mechanism is arranged in the shell, a conveying mechanism is arranged on the shell, the turnover mechanism comprises a turnover block and a stop block, protrusions are arranged at two ends of the turnover block, the stop block is rotatably connected with a shaking mechanism, a Y-shaped notch is formed in the stop block, the stop block notch and a turnover table form a Y-shaped track, the lower surface of the turnover table is lower than the lower surface of the stop block, the protrusions of the turnover block move along the track, the shaking mechanism is arranged on the turnover block and drives the shaking mechanism to overturn, and the turnover block is fixedly connected with the shaking mechanism.
Further, the transportation mechanism comprises a plurality of clamping assemblies, and the clamping assemblies are realized through a parallelogram connecting rod structure.
Furthermore, the clamping assembly comprises a clamping part, a conveying assembly is arranged in the clamping part, and the conveying assembly is matched with the clamping assembly to convey the test tube into the shaking-up mechanism.
Furthermore, the conveying assembly comprises a third motor, the third motor is fixedly installed on the clamping component, a plurality of small transmission shafts are installed in the clamping component in a rotating mode, one of the small transmission shafts is fixedly connected with a rotating shaft of the third motor, and a conveying belt is sleeved on the plurality of small transmission shafts.
Further, shake even mechanism and include rotating assembly and fixed subassembly, fixed subassembly includes support housing, is equipped with a plurality of slider-crank mechanisms on the support housing, slider-crank mechanism is used for fixing the test tube in support housing axle center.
Furthermore, the slider-crank mechanism comprises an eccentric shaft, the eccentric shaft is rotatably mounted on the support housing, the eccentric shaft is hinged to the cylinder wall of the cylinder, the cylinder is rotatably mounted on the support housing, the eccentric shaft is hinged to a push rod, the push rod is hinged to a chuck, a chute block is fixedly mounted on the support housing, and the chuck is slidably connected with the chute block.
Furthermore, the even shaking mechanism further comprises a connecting rod, wherein the first end of the connecting rod is hinged with the eccentric shaft, the second end of the connecting rod is hinged with the eccentric shaft in the adjacent crank block mechanism, and the crank block mechanism is driven by the connecting rod.
Furthermore, the supporting shell is divided into two layers, and each layer is provided with a plurality of groups of crank sliding block mechanisms.
Compared with the prior art, the invention has the beneficial effects that: (1) The conveying mechanism can realize the integration of clamping, conveying and disassembling the test tube, thereby reducing the operation time of workers and a large amount of labor force; (2) The shaking mechanism and the turnover mechanism are linked to realize the full shaking of the test tube, so that the problem of DNA damage caused by different forces during manual shaking is solved; (3) The fixing component can keep the test tube stable when the test tube is shaken uniformly, and reduces the damage of the test tube in the working process.
Drawings
FIG. 1 is an overall schematic view of the structure of the present invention.
Fig. 2 is a schematic view of the overall position of the present invention.
FIG. 3 is a schematic diagram of the turnover mechanism of the present invention.
Fig. 4 is a schematic view of the transport mechanism of the present invention.
Fig. 5 is a partial schematic view of the transport mechanism of the present invention.
FIG. 6 is a schematic view of the shaking mechanism of the present invention.
FIG. 7 is a partial schematic view of the shake-up mechanism of the present invention.
Reference numbers: 1-a housing; 2-a transport mechanism; 3-shaking up the mechanism; 4-turning over mechanism; 201, a first motor; 202-a support table; 203-a screw rod; 204-a drive platform; 205-a guide bar; 206-motor two; 207-driving gear; 208-a working gear; 209-driving gear; 210-a motor housing; 211-a conveyor belt; 212-motor three; 213-a driven rod; 214-rotating levers; 215-an active lever; 216-small drive shaft; 217-rotating shaft; 218-bevel gear one; 219 — bevel gear two; 220-a belt; 221-driving shaft;
222-a driven shaft; 223-a conveyor belt housing; 301-a cylinder; 302-a connecting rod; 303-an eccentric shaft; 304-a runner block; 305-a chuck;
306-a push rod; 307-connecting rod; 308-a support housing; 309-rotating disc; 310-motor four; 311-large bevel gear; 312-large driven bevel gear; 313-a rotating shaft; 401-turning over the block; 402-a flipping table; 403-a stopper; 404-support plate.
Detailed Description
The technical scheme of the invention is further explained by the specific implementation mode in combination with the attached drawings.
Wherein the showings are for the purpose of illustration only and are shown by way of illustration only and not in actual form, and are not to be construed as limiting the present patent; for a better explanation of the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
Example (b): as shown in figures 1 and 3, the automatic reverse and uniform mixing rapid DNA extraction device comprises a shell 1, a turnover mechanism 4 is arranged in the shell 1, a shaking mechanism 3 is arranged on the turnover mechanism 4, a transportation mechanism 2 is arranged on the shell 1, the turnover mechanism 4 comprises a turnover block 401 and a stop block 403, protrusions are arranged at two ends of the turnover block 401, the turnover block 401 is fixedly connected with the shaking mechanism 3, the stop block 403 is rotatably connected with the shaking mechanism 3, a Y-shaped notch is arranged on the stop block 403, a support plate 404 is fixedly arranged on the stop block 403, a turnover table 402 is fixedly arranged on the support plate 404, the turnover table 402 and the stop block 403 form a Y-shaped track, the lower surface of the turnover table 402 is lower than that of the stop block, the protrusions of the turnover block 401 move along the track and drive the shaking mechanism 3 to turn over.
As shown in fig. 1, the housing 1 has an outer shape of a circular housing, and three supports are installed at a lower portion thereof for stable support.
As shown in fig. 4 and 5, the transportation mechanism 2 includes a first motor 201, a support table 202 is fixedly installed on the first motor 201, the support table 202 is fixedly connected with the housing 1, a spiral rod 203 is fixedly installed at an output end of the first motor 201, a driving platform 204 is installed on the spiral rod 203 in a matching manner, two guide rods 205 are fixedly installed on the support table 202, the driving platform 204 is slidably connected with the guide rods 205, a motor housing 210 is fixedly installed at the other end of the turnover table 402, a second motor 206 is fixedly installed on the motor housing 210, a driving gear 207 is fixedly installed at an output end of the second motor 206, the driving gear 207 is engaged with a driving gear 209, and three clamping assemblies are installed on the driving gear 209; the first motor 201 starts to drive the screw rod 203 to rotate, the screw rod 203 rotates to drive the driving platform 204 to move up and down along the guide rod 205, the second motor 206 starts to drive the driving gear 207 to rotate, the driving gear 207 rotates to drive the driving gear 209 to rotate, and the driving gear 209 rotates to drive the three clamping assemblies to approach the center of the circle of the driving gear 209.
As shown in fig. 4 and 5, the clamping assembly includes a working gear 208, the working gear 208 is engaged with a driving gear 209, a rotating shaft 217 is fixedly installed on the inner surface of the driving gear 207, a first bevel gear 218 is fixedly installed on the outer surface of the rotating shaft 217, the first bevel gear 218 is engaged with a second bevel gear 219, a driving shaft 221 is fixedly installed on the inner surface of the second bevel gear 219, a belt 220 is sleeved on the driving shaft 221, driven rods 213 are rotatably installed on both sides of the driving shaft 221, a conveyor belt 211 is rotatably installed on a driving platform 204, rotating rods 214 are rotatably installed on both sides of the conveyor belt 211, the other ends of each two rotating rods 214 are rotatably connected with the driven shaft 222, a belt 220 is sleeved on the driven shaft 222, driven rods 213 are rotatably installed on both sides of the driven shaft 222, and a clamping component is installed on the other side of each two driven rods 213 on the same side; the driving gear 209 rotates to drive the working gear 208, the working gear 208 rotates to drive the rotating shaft 217 to rotate, the rotating shaft 217 rotates to drive the first bevel gear 218 to rotate, the first bevel gear 218 rotates to drive the second bevel gear 219 to rotate, the second bevel gear 219 rotates to drive the driving shaft 221 to rotate, the driving shaft 221 rotates to drive the belt 220 to rotate, the belt 220 rotates to drive the driven shaft 222 to rotate, the driven shaft 222 rotates to drive the rotating rods 214 and the driven rods 213 on two sides to rotate, the belt 220 rotates to drive the rotating rods 214 and the driven rods 213 on two sides to rotate, synchronous rotation increases efficiency, and the rotating rods 214 and the driven rods 213 rotate to drive the clamping part to move.
As shown in fig. 4 and 5, the holding member includes a belt housing 223, the belt housing 223 is triangular, the belt housing 223 is rotatably mounted with the other side of the driven rod 213 on the same side, and a conveying assembly is rotatably mounted between the two belt housings 223.
As shown in fig. 4 and 5, the conveying assembly includes a motor iii 212, the motor iii 212 is fixedly mounted on the clamping member, a plurality of small transmission shafts 216 are rotatably mounted in the clamping member, one of the small transmission shafts 216 is fixedly connected with a rotating shaft of the motor iii 212, and the plurality of small transmission shafts 216 are sleeved with the conveyor belt 211; the motor III 212 rotates to drive the small transmission shaft 216 to rotate. As shown in fig. 6 and 7, the rotating assembly includes a fourth motor 310, the fourth motor 310 is fixedly mounted on the housing 1, a large bevel gear 311 is fixedly mounted at an output end of the fourth motor 310, the large bevel gear 311 is engaged with a large driven bevel gear 312, a rotating shaft 313 is fixedly mounted on an inner surface of the large driven bevel gear 312, a rotating disc 309 is fixedly mounted on the rotating shaft 313, the rotating shaft 313 is rotatably mounted with a stopper 403, a connecting rod 302 is fixedly mounted on the rotating disc 309, one end of the connecting rod 302 is fixedly connected with the supporting housing 308, and the other end of the connecting rod 302 is fixedly connected with the turning block 401; the motor four 310 rotates to drive the large bevel gear 311 to rotate, the large bevel gear 311 rotates to drive the large driven bevel gear 312 to rotate, the large driven bevel gear 312 rotates to drive the rotating shaft 313 to rotate, the large driven bevel gear 312 rotates to drive the rotating disc 309 to rotate, the rotating disc 309 rotates to drive the connecting rod 302 to move, and the connecting rod 302 moves to drive the overturning block 401 and the supporting shell 308 to move.
As shown in fig. 6 and 7, the slider-crank mechanism includes a support housing 308, an air cylinder 301 rotatably mounted on the support housing 308, an eccentric shaft 303 hinged on a cylinder wall of the air cylinder 301, the eccentric shaft 303 rotatably mounted on the support housing 308, a push rod 306 hinged on the eccentric shaft 303, a chuck 305 hinged on the push rod 306, a chute block 304 fixedly mounted on the support housing 308, and the chuck 305 slidably connected with the chute block 304; the air cylinder 301 is started to push the eccentric shaft 303 to rotate, the eccentric shaft 303 rotates to drive the push rod 306 to move, and the push rod 306 moves to drive the chuck 305 to move.
As shown in fig. 6 and 7, the shake-up mechanism 3 further comprises a connecting rod 307, a first end of the connecting rod 307 is hinged to the eccentric shaft 303, a second end of the connecting rod 307 is hinged to the eccentric shaft 303 in the adjacent crank block mechanism, and the crank block mechanism is driven by the connecting rod 307; the rotation of the eccentric shafts 303 causes the movement of the connecting rods 307, and the movement of the connecting rods 307 causes the rotation of the adjacent eccentric shafts 303.
As shown in fig. 6 and 7, the support housing 308 is divided into two layers, and each layer is provided with three sets of slider-crank mechanisms.
The working principle is as follows: placing the test tube which is not shaken evenly into the center of three clamping parts, starting a second motor 206 to drive a driving gear 207 to rotate, driving the driving gear 207 to rotate to drive a driving gear 209 to rotate, driving the three clamping components to clamp the test tube by driving a first motor 201 to rotate, driving a spiral rod 203 to rotate, driving a platform 204 to move along a guide rod 205 by driving the spiral rod 203 to rotate, starting a third motor 212 after the test tube moves to a proper position, driving a small transmission shaft 216 to rotate by driving the third motor 212 to transport the test tube to the center of a support shell 308, starting a first rear motor 301 to drive an eccentric shaft 303 to rotate, driving a connecting rod 307 to rotate by driving the eccentric shaft 303 to rotate, driving an adjacent eccentric shaft 303 to rotate by driving a connecting rod 307 to rotate, driving a push rod 306 to move, driving a whole chuck 305 to clamp the test tube by driving a fourth motor 310 to rotate, driving a large bevel gear 311 to rotate by driving a large driven bevel gear 311 to rotate, driving a large driven bevel gear 312 to rotate, driving a turntable 309 to drive a connecting rod 302 to move, driving a connecting rod 401 and a support shell 308 to move, driving a spiral rod 203 to rotate a rotary rod 203 to rotate, driving a rotary rod 303 to rotate along a guide rod 203, driving a spiral rod 303 to rotate, driving a rotary rod 203 to rotate, driving a rotary rod 303 after the eccentric shaft to rotate, driving a rotary rod 305 to rotate, the second rear motor 206 is started to drive the driving gear 207 to rotate, the driving gear 207 rotates to drive the driving gear 209 to rotate, and the driving gear 209 rotates to drive the three clamping assemblies to clamp the test tube, so that the test tube is placed back to the original position.

Claims (4)

1. The utility model provides an automatic reverse quick DNA extraction element of mixing which characterized in that: the turnover mechanism comprises a shell (1), a turnover mechanism (4) is arranged in the shell (1), a conveying mechanism (2) is arranged on the shell (1), the turnover mechanism (4) comprises a turnover block (401) and a stop block (403), protrusions are arranged at two ends of the turnover block (401), the stop block (403) is rotatably connected with a shaking mechanism (3), a Y-shaped notch is formed in the stop block (403), the notch of the stop block (403) and a turnover table (402) form a Y-shaped track, the lower surface of the turnover table (402) is lower than the lower surface of the stop block (403), the protrusions of the turnover block (401) move along the track, the shaking mechanism (3) is arranged on the turnover block (401) and drives the shaking mechanism (3) to turn over, and the turnover block (401) is fixedly connected with the shaking mechanism (3); the conveying mechanism (2) comprises a plurality of clamping assemblies, and the clamping assemblies are clamped through a parallelogram connecting rod structure; the clamping component comprises a clamping component, a conveying component is arranged in the clamping component, and the conveying component is matched with the clamping component to convey the test tube into the shaking mechanism (3); the conveying assembly comprises a motor III (212), the motor III (212) is fixedly arranged on the clamping component, a plurality of small transmission shafts (216) are arranged in the clamping component in a rotating mode, one of the small transmission shafts (216) is fixedly connected with a rotating shaft of the motor III (212), and a conveying belt (211) is sleeved on the plurality of small transmission shafts (216); the shaking up mechanism (3) comprises a rotating assembly and a fixing assembly, the fixing assembly comprises a supporting shell (308), a plurality of slider-crank mechanisms are arranged on the supporting shell (308), and the slider-crank mechanisms are used for fixing the test tube on the axis of the supporting shell (308).
2. The automatic reverse rapid DNA extraction device for uniform mixing of claim 1, wherein the slider-crank mechanism comprises an eccentric shaft (303), the eccentric shaft (303) is rotatably mounted on the support housing (308), the eccentric shaft (303) is hinged with the wall of a cylinder (301), the cylinder (301) is rotatably mounted on the support housing (308), the eccentric shaft (303) is hinged with a push rod (306), the push rod (306) is hinged with a chuck (305), the support housing (308) is fixedly mounted with a chute block (304), and the chuck (305) is slidably connected with the chute block (304).
3. The apparatus for rapid DNA extraction with automatic reverse mixing as claimed in claim 2, wherein the shaking mechanism (3) further comprises a connecting rod (307), a first end of the connecting rod (307) is hinged to the eccentric shaft (303), a second end of the connecting rod (307) is hinged to the eccentric shaft (303) of the adjacent crank block mechanism, and the crank block mechanism is driven by the connecting rod (307).
4. The rapid DNA extraction device with automatic reverse blending according to claim 2, wherein the support housing (308) is divided into two layers, and each layer is provided with a plurality of groups of crank slider mechanisms.
CN202210223047.9A 2022-03-07 2022-03-07 Automatic reverse quick DNA extraction element of mixing Active CN114480073B (en)

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CN201746111U (en) * 2010-07-23 2011-02-16 中国重型机械研究院有限公司 Automatic material inlet and outlet mechanism
JP2012217954A (en) * 2011-04-11 2012-11-12 Iguchi Kiko Seisakusho:Kk Shaking device
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CN215540326U (en) * 2021-09-06 2022-01-18 南阳医学高等专科学校第一附属医院 Test tube shakes even device for endocrinology department
CN114100448A (en) * 2021-10-15 2022-03-01 肖耀东 Blood sampling shaking device for hematology department
CN114193474A (en) * 2021-12-21 2022-03-18 中国科学院自动化研究所 XiLin bottle joins in marriage liquid mechanism, automatic liquid system of joining in marriage

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