CN114469844A - Antibacterial microneedle and preparation method thereof - Google Patents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0021—Intradermal administration, e.g. through microneedle arrays, needleless injectors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/38—Silver; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
- A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Abstract
The invention discloses an antibacterial microneedle and a preparation method thereof. According to the technical scheme, the poly-dopamine coating with adhesiveness and reducibility formed by dopamine self-assembly is utilized to reduce the antibacterial metal ions in situ and attach the antibacterial metal ions to the surface of the microneedle so as to realize the antibacterial function of the microneedle.
Description
Technical Field
The application relates to the field of medical cosmetology, in particular to an antibacterial microneedle and a preparation method thereof.
Background
The microneedle is a needle-shaped protrusion array with the size of micron, and is mainly used for transdermal delivery of drugs; it is as effective as a hypodermic needle and as convenient as a conventional transdermal patch, and has been one of the research hotspots in the field of formulation.
While microneedles are painless, minimally invasive techniques, some microneedles for sustained drug delivery require a longer period of wear by the user, a process that can cause infection of the wound. Most of the existing microneedles do not have antibacterial ability, and the existing microneedles need to be sterilized by alcohol or steam before use and also need to be used together with antibiotics, so that wound infection of users is avoided, and the existing microneedles are troublesome.
Disclosure of Invention
In view of the above problems, the present application provides an antibacterial microneedle having an antibacterial function and a method for preparing the same.
In order to achieve the above object, the inventors provide a method for preparing an antibacterial microneedle, in which a polydopamine coating is formed on the surface of a base microneedle, and antibacterial metal ions are reduced in situ and attached to the surface of the polydopamine coating to obtain the antibacterial microneedle.
Different from the prior art, the technical scheme utilizes the poly-dopamine coating which is formed by dopamine self-assembly and has adhesiveness and reducibility to reduce the antibacterial metal ions in situ and attach the antibacterial metal ions to the surface of the microneedle so as to realize the antibacterial function of the microneedle.
In some embodiments, the method for preparing the antibacterial microneedle comprises the following steps:
and (3) forming a coating: placing a basic microneedle in a dopamine solution, and forming a polydopamine coating by self-assembling dopamine on the surface of the basic microneedle;
reduction and adhesion: washing the microneedle with the formed polydopamine coating by deionized water, soaking the washed microneedle in a solution containing antibacterial metal ions, and reducing the antibacterial metal ions in situ and attaching the antibacterial metal ions to the surface of the polydopamine coating under the condition of keeping away from light at 20-30 ℃ to obtain a semi-finished antibacterial microneedle;
drying: and cleaning the semi-finished antibacterial microneedle by using deionized water, and drying at constant temperature to obtain the antibacterial microneedle.
In some embodiments, in the coating formation step, the dopamine solution has a pH of 5.0 to 9.0 and a concentration of 0.1 to 10 mg/mL.
In some embodiments, the coating forming step, the ambient temperature of the coating forming step is 4 to 50 ℃, and the coating forming time is 1 to 48 hours.
In some embodiments, in the reducing and adhering step, the antibacterial metal ion is present in an amount of 0.01 to 100 mM.
In some embodiments, the antimicrobial metal ions include silver ions and copper ions.
In some embodiments, in the reducing and adhering step, the microneedle is soaked in the solution for 2-48 h.
In some embodiments, the drying temperature is 30-90 ℃.
In some embodiments, the base microneedle comprises a sponge microneedle.
The inventor also provides an antibacterial microneedle which is prepared by adopting any one of the methods; the surface of the antibacterial microneedle is coated with a polydopamine coating, and antibacterial metal elements are attached to the surface of the polydopamine coating.
Different from the prior art, the technical scheme utilizes the poly-dopamine coating which is formed by dopamine self-assembly and has adhesiveness and reducibility to reduce the antibacterial metal ions in situ and attach the antibacterial metal ions to the surface of the microneedle so as to realize the antibacterial function of the microneedle.
The above description of the present invention is only an overview of the technical solutions of the present application, and in order to make the technical solutions of the present application more clearly understood by those skilled in the art, the present invention may be further implemented according to the content described in the text and drawings of the present application, and in order to make the above objects, other objects, features, and advantages of the present application more easily understood, the following description is made in conjunction with the detailed description of the present application and the drawings.
Drawings
The drawings are only for purposes of illustrating the principles, implementations, applications, features, and effects of particular embodiments of the present application, as well as others related thereto, and are not to be construed as limiting the application.
In the drawings of the specification:
FIG. 1 is a schematic view of the process for preparing the antibacterial sponge microneedles of examples 1 to 5;
fig. 2 is a graph showing the results of the antibiotic test of the antibiotic sponge microneedle and the base microneedle prepared in examples 1 to 5.
Detailed Description
In order to explain in detail possible application scenarios, technical principles, practical embodiments, and the like of the present application, the following detailed description is given with reference to the accompanying drawings in conjunction with the listed embodiments. The embodiments described herein are merely for more clearly illustrating the technical solutions of the present application, and therefore, the embodiments are only used as examples, and the scope of the present application is not limited thereby.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase "an embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or related to other embodiments specifically defined. In principle, in the present application, the technical features mentioned in the embodiments can be combined in any manner to form a corresponding implementable technical solution as long as there is no technical contradiction or conflict.
Unless defined otherwise, technical terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the use of relational terms herein is intended only to describe particular embodiments and is not intended to limit the present application.
In the description of the present application, the term "and/or" is a expression for describing a logical relationship between objects, indicating that three relationships may exist, for example, a and/or B, indicating that: there are three cases of A, B, and both A and B. In addition, the character "/" herein generally indicates that the former and latter associated objects are in a logical relationship of "or".
In this application, terms such as "first" and "second" are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions.
Without further limitation, in this application, the use of "including," "comprising," "having," or other similar expressions in phrases and expressions of "including," "comprising," or "having," is intended to cover a non-exclusive inclusion, and such expressions do not exclude the presence of additional elements in a process, method, or article that includes the recited elements, such that a process, method, or article that includes a list of elements may include not only those elements but also other elements not expressly listed or inherent to such process, method, or article.
As is understood in the examination of the guidelines, the terms "greater than", "less than", "more than" and the like in this application are to be understood as excluding the number; the expressions "above", "below", "within" and the like are understood to include the present numbers. In addition, in the description of the embodiments of the present application, "a plurality" means two or more (including two), and expressions related to "a plurality" similar thereto are also understood, for example, "a plurality of groups", "a plurality of times", and the like, unless specifically defined otherwise.
In the description of the embodiments of the present application, spatially relative expressions such as "central," "longitudinal," "lateral," "length," "width," "thickness," "up," "down," "front," "back," "left," "right," "vertical," "horizontal," "vertical," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used, and the indicated orientations or positional relationships are based on the orientations or positional relationships shown in the specific embodiments or drawings and are only for convenience of describing the specific embodiments of the present application or for the convenience of the reader, and do not indicate or imply that the device or component in question must have a specific position, a specific orientation, or be constructed or operated in a specific orientation and therefore should not be construed as limiting the embodiments of the present application.
Unless specifically stated or limited otherwise, the terms "mounted," "connected," "secured," and "disposed" used in the description of the embodiments of the present application are to be construed broadly. For example, the connection can be a fixed connection, a detachable connection, or an integrated arrangement; it can be a mechanical connection, an electrical connection, or a communication connection; they may be directly connected or indirectly connected through an intermediate; which may be communication within two elements or an interaction of two elements. Specific meanings of the above terms in the embodiments of the present application can be understood by those skilled in the art to which the present application pertains in accordance with specific situations.
Firstly, the inventor provides a preparation method of an antibacterial microneedle, and the antibacterial microneedle is obtained by forming a polydopamine coating on the surface of a basic microneedle, carrying out in-situ reduction on antibacterial metal ions and attaching the antibacterial metal ions to the surface of the polydopamine coating.
Different from the prior art, the technical scheme utilizes the poly-dopamine coating which is formed by dopamine self-assembly and has adhesiveness and reducibility to reduce the antibacterial metal ions in situ and attach the antibacterial metal ions to the surface of the microneedle so as to realize the antibacterial function of the microneedle.
The dopamine has good stability and biocompatibility, does not damage skin and has no skin irritation, so the dopamine serving as a biological material is suitable for preparing the micro-needle. The dopamine will undergo automatic assembly to form a polydopamine film. The surface of the polydopamine film coating contains reductive phenolic hydroxyl and amino active groups which can be combined with antibacterial metal ions to reduce the antibacterial metal ions. In addition, the polydopamine coating also has adhesiveness, so that the combination of the antibacterial metal elements (including the metal particles in an ionic state and after reduction) and the surface of the polydopamine coating is firmer. The technical scheme can also ensure that the antibacterial metal elements are uniformly distributed on the surface of the micro-needle.
In some embodiments, the method for preparing the antibacterial microneedle comprises the following steps:
and (3) forming a coating: placing a basic microneedle in a dopamine solution, and forming a polydopamine coating by self-assembling dopamine on the surface of the basic microneedle;
reduction and adhesion: washing the microneedle with the formed polydopamine coating by deionized water, soaking the washed microneedle in a solution containing antibacterial metal ions, and reducing the antibacterial metal ions in situ and adhering the antibacterial metal ions to the surface of the polydopamine coating under the condition of keeping away from light at the temperature of 20-30 ℃ to obtain a semi-finished antibacterial microneedle;
drying: and cleaning the semi-finished antibacterial microneedle by using deionized water, and drying at constant temperature to obtain the antibacterial microneedle.
The base microneedles described in the present embodiment are commercially available various finished microneedles. In the technical scheme, the antibacterial microneedle can be directly reprocessed on the basis of various commercially available microneedles with different structures or functions, the production and processing line of the original microneedle does not need to be modified, the process is simple, and the cost is saved.
In some embodiments, in the coating formation step, the dopamine solution has a pH of 5.0 to 9.0 and a concentration of 0.1 to 10 mg/mL. In some preferred embodiments, the dopamine solution has a pH of 8.5 and a dopamine solution concentration of 2 mg/mL.
The pH of the dopamine solution has an effect on the degree and speed of dopamine self-assembly, and alkaline conditions favor its self-assembly, preferably pH 8.5. The concentration of the dopamine solution has an effect on the thickness of the polydopamine coating, too thin a coating is prone to rupture during handling, and too thick a coating is detrimental to skin injection. A preferred embodiment is 2 mg/mL.
In some embodiments, the polydopamine coating has a thickness of 20-50 nm.
In some embodiments, the coating forming step, the ambient temperature of the coating forming step is 4 to 50 ℃, and the coating forming time is 1 to 48 hours. In a preferred embodiment, the ambient temperature for the coating formation is 37 ℃ and the coating formation time is 6 hours. The self-assembly time of the dopamine solution also affects the thickness of the polydopamine coating, too thin a coating is prone to break during handling, and too thick a coating is detrimental to skin injections.
In some embodiments, the coating formation step, the formation of a polydopamine coating, still results in the presence of some amount of monomeric dopamine and/or intermediate dopamine quinone. It can be combined with antibacterial metal ions in the subsequent reduction and adhesion steps to be further oxidized.
In some embodiments, the antimicrobial metal ions include silver ions and copper ions. In other embodiments, the antimicrobial metal ions further include, but are not limited to, zinc ions and Ce ions. Antibacterial effect against bacteria: ag, Cu, Zn and Ce.
In some embodiments, in the reducing and adhering step, the antibacterial metal ion is present in an amount of 0.01 to 100 mM. In some preferred embodiments, in the reducing and adhering step, the antibacterial metal ion is contained in an amount of 0.01 to 10 mM. In a further preferred embodiment, in the reducing and adhering step, the content of the antibacterial metal ions is 2 mM.
The action mechanism of the antibacterial metal is a 'surface layer action', that is, the surface of the microneedle can realize the expected antibacterial effect as long as the surface of the microneedle meets a certain content of the antibacterial metal element, and the antibacterial effect cannot be increased by the antibacterial metal element with an excessively high content, but the raw material cost is wasted, and the quality of the antibacterial microneedle is increased, which is not expected.
In some embodiments, in the reducing and adhering step, the microneedle is soaked in the solution for 2-48 h. In a preferred embodiment, the microneedle is soaked in the solution for 12 hours. The immersion time of the microneedles in the solution can affect the amount of antimicrobial metal ion attached. If the amount of the antibacterial metal ions is too low, the expected antibacterial effect cannot be achieved; the amount of the antibacterial metal ions is too much, and the antibacterial effect cannot be increased by the high content of the antibacterial metal elements, but the raw material cost is wasted, and the quality of the antibacterial microneedle is increased, which is not expected.
In some embodiments, the constant temperature drying temperature is 30-90 ℃. The drying temperature is generally determined according to factors such as different components of the microneedle, whether the microneedle contains the drug or not, and the like. The drying temperature needs to be controlled, and the constant temperature state is kept in the drying process, so that the physical or chemical change of the micro-needle is avoided.
In some embodiments, the base microneedle is a polyethylene microneedle.
In some embodiments, the base microneedle is a polyvinyl alcohol sponge microneedle.
The inventor also provides an antibacterial microneedle which is prepared by adopting any one of the methods; the surface of the antibacterial microneedle is coated with a polydopamine coating, and antibacterial metal elements are attached to the surface of the polydopamine coating.
Different from the prior art, the antibacterial metal ions are reduced in situ and attached to the surface of the microneedle by utilizing the polydopamine coating formed by dopamine self-assembly in the technical scheme so as to realize the antibacterial function of the microneedle.
Examples
In order to further clarify the explanation and explanation of the technical solution of the present application, the following non-limiting examples and fig. 1 are also provided for reference. The embodiments of the present application have been made in an effort to ensure the accuracy of the numerical values, but some errors and deviations should be accounted for.
The basic microneedles used in this embodiment are all polyvinyl alcohol sponge microneedles produced in the same production batch, and are prepared according to the technical scheme disclosed in the granted chinese patent with application number 201610494137.6 and named as a preparation method of polyvinyl alcohol sponge microneedles.
Example 1 an antibacterial sponge microneedle
And (3) forming a coating: placing the basic sponge microneedle into a dopamine solution with the pH value of 5 and the concentration of 1mg/mL, and reacting for 12 hours at 4 ℃ to allow dopamine to perform self-assembly on the surface of the sponge microneedle to form a polydopamine coating;
reduction and adhesion: taking out the sponge microneedle coated with polydopamine, washing with deionized water for 2-5 times, soaking in 1mM silver nitrate solution, and reacting at 25 deg.C in dark place for 8 hr to obtain semi-finished antibacterial sponge microneedle;
drying: and taking out the semi-finished antibacterial sponge microneedle, washing the semi-finished antibacterial sponge microneedle for 2-5 times by using deionized water, and drying the semi-finished antibacterial sponge microneedle in a constant-temperature drying box at 40 ℃ to obtain the antibacterial sponge microneedle 1 with the surface containing nano silver particles.
Example 2 an antibacterial sponge microneedle
And (3) forming a coating: placing the basic sponge microneedle into a dopamine solution with the pH value of 7 and the concentration of 0.1mg/mL, and reacting for 24 hours at 25 ℃ to allow dopamine to perform self-assembly on the surface of the sponge microneedle to form a polydopamine coating;
reduction and adhesion: taking out the sponge microneedle coated with polydopamine, washing with deionized water for 2-5 times, respectively soaking in 10mM silver nitrate solution, and reacting at 25 deg.C in dark for 18 hr to obtain semi-finished antibacterial sponge microneedle;
drying: and taking out the semi-finished antibacterial sponge microneedle, washing the semi-finished antibacterial sponge microneedle for 2-5 times by using deionized water, and drying the semi-finished antibacterial sponge microneedle in a constant-temperature drying box at 50 ℃ to obtain the antibacterial sponge microneedle 2 with the surface containing nano silver particles.
Example 3 an antibacterial sponge microneedle
And (3) forming a coating: placing the basic sponge microneedle into a dopamine solution with the pH value of 8.5 and the concentration of 2mg/mL, and reacting for 6 hours at 37 ℃ to allow dopamine to perform self-assembly on the surface of the sponge microneedle to form a polydopamine coating;
reduction and adhesion: taking out the sponge microneedle coated with polydopamine, washing with deionized water for 2-5 times, respectively soaking in silver nitrate solution with concentration of 2mM, and reacting at 25 deg.C in dark for 12 hr to obtain semi-finished antibacterial sponge microneedle;
drying: and taking out the semi-finished antibacterial sponge microneedle, washing the semi-finished antibacterial sponge microneedle for 2-5 times by using deionized water, and drying the semi-finished antibacterial sponge microneedle in a constant-temperature drying box at 60 ℃ to obtain the antibacterial sponge microneedle 3 with the surface containing nano silver particles.
Example 4 an antibacterial sponge microneedle
And (3) forming a coating: placing the basic sponge microneedle into a dopamine solution with the pH value of 9 and the concentration of 10mg/mL, and reacting for 48 hours at 50 ℃ to allow dopamine to perform self-assembly on the surface of the sponge microneedle to form a polydopamine coating;
reduction and adhesion: taking out the sponge microneedle coated with polydopamine, washing with deionized water for 2-5 times, respectively soaking in 50mM silver nitrate solution, and reacting at 25 deg.C in dark for 48 hr to obtain semi-finished antibacterial sponge microneedle;
drying: and taking out the semi-finished antibacterial sponge microneedle, washing the semi-finished antibacterial sponge microneedle for 2-5 times by using deionized water, and drying the semi-finished antibacterial sponge microneedle in a constant-temperature drying box at 70 ℃ to obtain the antibacterial sponge microneedle 4 with the surface containing nano silver particles.
Example 5 an antibacterial sponge microneedle
And (3) forming a coating: placing the basic sponge microneedle into a dopamine solution with the pH value of 8.5 and the concentration of 10mg/mL, and reacting for 24 hours at 25 ℃ to allow dopamine to perform self-assembly on the surface of the sponge microneedle to form a polydopamine coating;
reduction and adhesion: taking out the sponge microneedle coated with polydopamine, washing with deionized water for 2-5 times, respectively soaking in 100mM silver nitrate solution, and reacting at 25 deg.C in dark place for 24 hr to obtain semi-finished antibacterial sponge microneedle;
drying: and taking out the semi-finished antibacterial sponge microneedle, washing the semi-finished antibacterial sponge microneedle for 2-5 times by using deionized water, and drying the semi-finished antibacterial sponge microneedle in a constant-temperature drying box at 90 ℃ to obtain the antibacterial sponge microneedle 5 with the surface containing nano silver particles.
The antibacterial sponge microneedles prepared in examples 1 to 5 and the basic ordinary sponge microneedles were subjected to antibacterial tests:
the test method is as follows: placing the sterilized groups of samples (common sponge microneedle and antibacterial sponge microneedle 1-5) in the center of 24-well plate, and dripping 1mL of Staphylococcus aureus with density of 10 into each well5Incubating CFU/mL bacterial solution with sample at 37 deg.C for 24 hr, and collecting 150 μ L bacterial solution per well at 660nm (OD)660) The optical density value was measured. The turbidity of each solution is closely related to the number of bacteria in the solution, and the larger the OD value, the lower the clarity of the solution system, and the larger the number of bacteria. Each set was made in triplicate (n-3).
By measuring the optical density value (OD) at 660nm660) To reflect the proliferation of bacteria in different solution systems, OD660Larger values indicate more bacteria present.
The results are shown in FIG. 2. It can be found that 5 kinds of antibacterial sponge microneedles have antibacterial effects, and have significant differences compared with common sponge microneedles (basic microneedles) in the control. Among them, the antibacterial sponge microneedle 3 has the lowest absorbance and shows the best antibacterial performance.
Finally, it should be noted that, although the above embodiments have been described in the text and drawings of the present application, the scope of the patent protection of the present application is not limited thereby. All technical solutions which are generated by replacing or modifying the equivalent structure or the equivalent flow according to the contents described in the text and the drawings of the present application, and which are directly or indirectly implemented in other related technical fields, are included in the scope of protection of the present application.
Claims (10)
1. The preparation method of the antibacterial microneedle is characterized in that a polydopamine coating is formed on the surface of a basic microneedle, antibacterial metal ions are subjected to in-situ reduction and attached to the surface of the polydopamine coating, and the antibacterial microneedle is obtained.
2. The method for preparing an antibacterial microneedle according to claim 1, comprising the steps of:
and (3) forming a coating: placing a basic microneedle in a dopamine solution, and forming a polydopamine coating by self-assembling dopamine on the surface of the basic microneedle;
reduction and adhesion: washing the microneedle with the formed polydopamine coating by using deionized water, soaking the washed microneedle in a solution containing antibacterial metal ions, and reducing the antibacterial metal ions in situ and adhering the antibacterial metal ions to the surface of the polydopamine coating under the condition of keeping away from light at 20-30 ℃ to obtain a semi-finished antibacterial microneedle;
drying: and cleaning the semi-finished antibacterial microneedle by using deionized water, and drying at constant temperature to obtain the antibacterial microneedle.
3. The method of manufacturing antibacterial microneedles in claim 2, wherein in the coating layer forming step, the pH of the dopamine solution is 5.0-9.0 and the concentration is 0.1-10 mg/mL.
4. A method of preparing an antibacterial microneedle according to claim 2, wherein said coating layer is formed at an ambient temperature of 4-50 ℃ for 1-48 hours.
5. The method of manufacturing an antibacterial microneedle according to claim 2, wherein in the reducing and adhering steps, the content of the antibacterial metal ion is 0.01 to 100 mM.
6. A method of manufacturing antibacterial microneedles as claimed in claim 2, wherein the antibacterial metal ions comprise silver ions and copper ions.
7. The method for preparing an antibacterial microneedle according to claim 2, wherein in the reducing and adhering steps, the microneedle is soaked in the solution for 2-48 hours.
8. The method of manufacturing antimicrobial microneedles in claim 2, wherein the drying step is performed at a temperature of 30-90 ℃.
9. The method of manufacturing antibacterial microneedles in any one of claims 1 to 8, wherein the base microneedles are polyvinyl alcohol sponge microneedles.
10. An antimicrobial microneedle, characterized in that it is produced by the method according to any one of claims 1 to 9; the surface of the antibacterial microneedle is coated with a polydopamine coating, and antibacterial metal elements are attached to the surface of the polydopamine coating.
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| CN115399756A (en) * | 2022-09-14 | 2022-11-29 | 华东理工大学 | Multi-parameter SERS active microneedle for in-situ detection of inflammatory environment |
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