CN114468299B - Nervonic acid-rich dietary supplement for promoting brain functions and preparation method and application thereof - Google Patents
Nervonic acid-rich dietary supplement for promoting brain functions and preparation method and application thereof Download PDFInfo
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- CN114468299B CN114468299B CN202210042112.8A CN202210042112A CN114468299B CN 114468299 B CN114468299 B CN 114468299B CN 202210042112 A CN202210042112 A CN 202210042112A CN 114468299 B CN114468299 B CN 114468299B
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- nervonic acid
- solution
- acer truncatum
- oil
- dietary supplement
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- GWHCXVQVJPWHRF-KTKRTIGZSA-N (15Z)-tetracosenoic acid Chemical compound CCCCCCCC\C=C/CCCCCCCCCCCCCC(O)=O GWHCXVQVJPWHRF-KTKRTIGZSA-N 0.000 title claims abstract description 70
- XJXROGWVRIJYMO-SJDLZYGOSA-N Nervonic acid Natural products O=C(O)[C@@H](/C=C/CCCCCCCC)CCCCCCCCCCCC XJXROGWVRIJYMO-SJDLZYGOSA-N 0.000 title claims abstract description 70
- GWHCXVQVJPWHRF-UHFFFAOYSA-N cis-tetracosenoic acid Natural products CCCCCCCCC=CCCCCCCCCCCCCCC(O)=O GWHCXVQVJPWHRF-UHFFFAOYSA-N 0.000 title claims abstract description 70
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- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 9
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- 238000000034 method Methods 0.000 claims description 8
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- 229960002675 xylitol Drugs 0.000 claims description 5
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 4
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 claims description 4
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- 229940083466 soybean lecithin Drugs 0.000 claims description 4
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- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 claims description 4
- 235000019202 steviosides Nutrition 0.000 claims description 4
- JBYXPOFIGCOSSB-GOJKSUSPSA-N 9-cis,11-trans-octadecadienoic acid Chemical compound CCCCCC\C=C\C=C/CCCCCCCC(O)=O JBYXPOFIGCOSSB-GOJKSUSPSA-N 0.000 claims description 3
- ZMJBYMUCKBYSCP-UHFFFAOYSA-N Hydroxycitric acid Chemical compound OC(=O)C(O)C(O)(C(O)=O)CC(O)=O ZMJBYMUCKBYSCP-UHFFFAOYSA-N 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 229940108924 conjugated linoleic acid Drugs 0.000 claims description 3
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- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 claims description 3
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
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- 150000001720 carbohydrates Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
- A23L33/12—Fatty acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/364—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/40—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds characterised by the fats used
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/48—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/022—Refining
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Health & Medical Sciences (AREA)
- Inorganic Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Mycology (AREA)
- Botany (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The application relates to a dietary supplement rich in nervonic acid for promoting brain function and a preparation method and application thereof, and belongs to the technical field of dietary supplements. The dietary supplement for promoting cerebral functions to be rich in nervonic acid, which is easy to oxidize and destroy in nutritive value, avoids the oxidation of oil containing nervonic acid through liposome inclusion, does not cause immune reaction after entering a body, protects the carried nervonic acid, and prevents body fluid from diluting nervonic acid and being destroyed by in-vivo enzyme; and then the liposome is modified by the abundant polar groups on the surface of the chitosan, so that the overall stability of the nervonic acid is improved.
Description
Technical Field
The application belongs to the technical field of dietary supplements, and particularly relates to a dietary supplement for promoting cerebral functions and enriching nervonic acid, and a preparation method and application thereof.
Background
Nervonic acid is divided into animal nervonic acid and autonomic nervonic acid, and human brain nervonic acid is animal nervonic acid, also called compound nervonic acid, is a core natural component of brain nerve cells and nerve tissues, and is a special substance for promoting repair and regeneration of damaged nerve cells and tissues. Foreign researches show that the nervonic acid is a core natural component of brain nerve cells and nerve tissues, is a special-effect substance which is discovered in the world so far and can promote the repair and regeneration of damaged nerve tissues, is a necessary 'high-grade nutrient' for the growth, regeneration and maintenance of nerve cells, especially brain cells, optic nerve cells and peripheral nerve cells, and has great effects of improving the activity degree of brain nerves and preventing the aging of the brain nerves. Domestic related researches show that the maximum content of nervonic acid in the garlic fruits is 62.6%, and the maximum content of nervonic acid in the garlic fruits is limited in industrial production due to narrow distribution zone and small resource amount of the garlic fruits. The content of nervonic acid in acer truncatum is 5% -6%, which is lower than that in garlic, but the result is large, the yield of nervonic acid is considerable, and acer truncatum has become one of the sustainable nervonic acid new resources.
The random control study of the influence of the addition of the nervonic acid food on the cognitive dysfunction of the Alzheimer disease shows that the addition of the nervonic acid food in the normal diet of people in daily life can increase the content of the nervonic acid in the human body. The prior art has few foods containing nervonic acid, so a dietary supplement rich in nervonic acid for promoting brain functions and a preparation method and application thereof are provided.
Disclosure of Invention
The application aims to solve the problems and provide a dietary supplement rich in nervonic acid for promoting brain functions, and a preparation method and application thereof.
The application realizes the above purpose through the following technical scheme:
the application provides a dietary supplement for promoting brain function and enriching nervonic acid, which is a mixture obtained by taking acer truncatum oil which is extracted from acer truncatum and contains 65-80% of nervonic acid as a main raw material, taking hawthorn kernel essential oil as an auxiliary material, emulsifying by an emulsifying agent, then clathrating by a chitosan modified liposome, and adding sugar blocking solution for mixing.
The application also provides a preparation method of the dietary supplement for promoting cerebral functions and enriching nervonic acid, which comprises the following steps:
(1) Acer truncatum seeds are taken as raw materials, and Acer truncatum grease containing 65-80% of nervonic acid is obtained through extraction;
(2) Adding chitosan modified liposome into absolute ethyl alcohol to obtain dispersion liquid, respectively adding emulsifying agent into the acer truncatum oil containing 65% -80% of nervonic acid and the hawthorn kernel essential oil, heating, stirring to obtain milk shake-shaped mixture, slowly dripping the two mixtures into the dispersion liquid, mixing, evaporating to remove the ethanol, filtering by a filter membrane, and drying at low temperature to obtain a product A;
(3) Dispersing the product A in the sugar blocking solution, fully mixing, filtering by a filter membrane, and drying at low temperature to obtain the finished product.
As a further improvement of the present application, the specific steps of the step (1) are as follows:
a. acer truncatum seeds are taken as raw materials to obtain Acer truncatum oil through supercritical extraction;
b. absolute ethyl alcohol and acer truncatum oil are mixed according to the volume ratio of 1: (1-10) adding 2-3wt% of catalyst into Acer truncatum Bunge oil, reacting at 50-80deg.C for 1-5 hr, removing unreacted ethanol, separating glycerol, and washing with water to obtain Acer truncatum Bunge oil.
As a further improvement of the present application, the catalyst is specifically sodium D-erythorbate.
As a further improvement of the application, the mass ratio of the acer truncatum oil containing 65-80% of nervonic acid to the hawthorn kernel essential oil in the step (2) is (2-5): 1.
as a further improvement of the present application, the emulsifier in the step (2) is one of food grade tween, span, monoglyceride or sucrose ester.
As a further improvement of the present application, the preparation steps of the chitosan-modified liposome in the step (2) are as follows:
a. dissolving soybean lecithin and cholesterol in absolute ethanol, and ultrasonically dissolving at below 0deg.C to obtain lipid solution;
b. dissolving chitosan in a mixed solution of acetic acid and phosphate buffer solution to obtain chitosan solution;
c. heating the lipid solution and the chitosan solution to 45-52 ℃, mixing, ultrasonic stirring, centrifuging and drying to obtain the chitosan modified liposome.
As a further improvement of the present application, the sugar blocking solution in the step (3) is prepared by: dissolving conjugated linoleic acid to prepare a solution, heating to 50-60 ℃, and then respectively mixing hydroxycitric acid, a starch blocking agent and an o Luo Li sugar blocking agent according to the mass ratio of 1 (2-3): (1-2) adding the mixture to the solution after sufficient mixing to obtain a sugar-blocking solution.
The application also provides application of the dietary supplement for promoting cerebral functions and enriching nervonic acid in health care products.
As a further improvement of the application, the health care product is gel candy, and the preparation method of the gel candy comprises the following steps:
(1) Adding 13-15% of glycerol, 30-35% of purified water, 14-16% of xylitol and 0.01-0.1% of stevioside into a gelatin dissolving tank according to mass percentage, uniformly stirring, heating to 60 ℃, adding 36-41% of gelatin, capping and sealing, stirring and heating to 75 ℃, stirring to form uniform gelatin solution, vacuumizing to remove bubbles in the gelatin solution, pressurizing to obtain gelatin, and sieving with a 100-mesh sieve to form capsule skin feed liquid;
(2) Adding the dietary supplement into DHA fish oil according to 1-2wt%, then pouring into the capsule shell feed liquid, drying, selecting pills, and removing unqualified pills such as abnormal pills, oil leakage pills and the like to obtain the gel candy.
The application has the beneficial effects that:
the dietary supplement for promoting cerebral functions to be rich in nervonic acid, which is easy to oxidize and has damaged nutritive value, avoids the oxidation of oil containing nervonic acid through liposome inclusion, does not cause immune reaction after entering a human body, protects the carried nervonic acid, prevents body fluid from diluting nervonic acid and being damaged by in-vivo enzyme, and simultaneously generates an emulsification-like effect in the liposome decomposition process after entering a human body, thereby solving the problems that nervonic acid in acer truncatum is difficult to dissolve in water and is difficult to be absorbed by the human body; the liposome is modified by the abundant polar groups on the surface of chitosan, so that the overall stability of the nervonic acid is improved, and the dietary supplement is not easy to be contaminated by bacteria during production, transportation and use by virtue of the sterilization and bacteriostasis effects, and can be rapidly identified by intestinal villus cells after entering a human body, so that the dietary supplement can be rapidly absorbed by the stomach and intestine; the nervonic acid and the hawthorn seed essential oil are utilized to cooperate with each other, so that the nervonic acid is easier to be absorbed; through mixing with sugar blocking solution, postprandial blood sugar response and fat absorption of eaters are reduced, and absorption of nervonic acid nutrient components is ensured.
The gel candy prepared by the application not only has good health care effect, but also has long preservation time and is easy to store.
Detailed Description
The following detailed description of the application is provided to illustrate the application and should not be construed as limiting the scope of the application since it is intended that the following detailed description is given for the purpose of illustration only, and that certain non-essential modifications and adaptations of the application may occur to those skilled in the art in light of the foregoing disclosure.
Example 1
The preparation method of the dietary supplement for promoting cerebral functions and enriching nervonic acid in the embodiment comprises the following steps:
(1) Preparation of Acer Truncatum Bunge grease
a. Acer truncatum seeds are taken as raw materials to obtain Acer truncatum oil through supercritical extraction;
b. absolute ethyl alcohol and acer truncatum oil are mixed according to the volume ratio of 1:4, adding 2-3wt% of D-sodium erythorbate into the Acer truncatum oil after mixing, reacting for 2.3 hours at 50 ℃, removing unreacted ethanol from the product, separating glycerol, washing with water to obtain Acer truncatum oil, and measuring the content of nervonic acid in the Acer truncatum oil by high performance liquid chromatography to 78.9%.
(2) Preparation of Chitosan-modified liposomes
a. Dissolving 10g of soybean lecithin with purity of 98% and 4g of cholesterol in 100mL of absolute ethyl alcohol, and performing ultrasonic dissolution at a temperature below 0 ℃ to prepare a lipid solution;
b. 2g-2.5g of chitosan is dissolved in a mixed solution of 100mL of acetic acid with volume concentration of 1%d and 100mL of phosphate buffer solution with pH of 7.0, so as to obtain chitosan solution;
c. heating the lipid solution and the chitosan solution to 45-52 ℃, mixing, ultrasonic stirring, centrifuging and drying to obtain the chitosan modified liposome.
(3) Preparation of sugar blocking solution
Dissolving 5g of conjugated linoleic acid to prepare a solution, heating to 50-60 ℃, and then respectively mixing the hydroxycitric acid, the starch blocker and the o Luo Li sugar blocker according to the mass ratio of 1:2: and 2, fully and uniformly mixing and adding the mixture into the solution to obtain the sugar blocking solution.
(4) Preparation of dietary supplements
Adding chitosan modified liposome into absolute ethyl alcohol to obtain dispersion liquid, respectively adding emulsifying agent into the acer truncatum oil containing 65% -80% of nervonic acid and the hawthorn kernel essential oil, and heating, wherein the mass ratio of the acer truncatum oil containing 65% -80% of nervonic acid to the hawthorn kernel essential oil is 2:1, stirring to obtain a milkshake-shaped mixture, slowly dripping the two mixtures into a dispersion liquid, mixing, evaporating to remove ethanol, filtering with a filter membrane, drying at low temperature to obtain a product A, dispersing the product A into a sugar blocking solution, fully mixing, filtering with the filter membrane, and drying at low temperature to obtain a finished product, wherein the emulsifier is one of food-grade tween, span, monoglyceride or sucrose ester, and the emulsifier in the embodiment selects food-grade toast.
Example 2
The preparation method of the dietary supplement for promoting cerebral functions and enriching nervonic acid in the embodiment comprises the following steps:
the procedure of example 1 is followed except (4) preparing a dietary supplement;
adding chitosan modified liposome into absolute ethyl alcohol to obtain dispersion liquid, respectively adding emulsifying agent into the acer truncatum oil containing 65% -80% of nervonic acid and the hawthorn kernel essential oil, and heating, wherein the mass ratio of the acer truncatum oil containing 65% -80% of nervonic acid to the hawthorn kernel essential oil is 4:1, stirring to obtain a milkshake-shaped mixture, slowly dripping the two mixtures into a dispersion liquid, mixing, evaporating to remove ethanol, filtering with a filter membrane, drying at low temperature to obtain a product A, dispersing the product A into a sugar blocking solution, fully mixing, filtering with the filter membrane, and drying at low temperature to obtain a finished product, wherein the emulsifier is one of food-grade tween, span, monoglyceride or sucrose ester, and the emulsifier in the embodiment selects food-grade toast.
Example 3
The preparation method of the dietary supplement for promoting cerebral functions and enriching nervonic acid in the embodiment comprises the following steps:
the procedure of example 1 is followed except (4) preparing a dietary supplement;
adding chitosan modified liposome into absolute ethyl alcohol to obtain dispersion liquid, respectively adding emulsifying agent into the acer truncatum oil containing 65% -80% of nervonic acid and the hawthorn kernel essential oil, and heating, wherein the mass ratio of the acer truncatum oil containing 65% -80% of nervonic acid to the hawthorn kernel essential oil is 5:1, stirring to obtain a milkshake-shaped mixture, slowly dripping the two mixtures into a dispersion liquid, mixing, evaporating to remove ethanol, filtering with a filter membrane, drying at low temperature to obtain a product A, dispersing the product A into a sugar blocking solution, fully mixing, filtering with the filter membrane, and drying at low temperature to obtain a finished product, wherein the emulsifier is one of food-grade tween, span, monoglyceride or sucrose ester, and the emulsifier in the embodiment selects food-grade toast.
Comparative example 1
The preparation method of the dietary supplement in the comparative example comprises the following steps:
(1) Preparation of Acer Truncatum Bunge grease
a. Acer truncatum seeds are taken as raw materials to obtain Acer truncatum oil through supercritical extraction;
b. absolute ethyl alcohol and acer truncatum oil are mixed according to the volume ratio of 1:4, adding sodium ethoxide with 2-3wt% of Acer truncatum Bunge oil after mixing, reacting for 5 hours at 50 ℃, removing unreacted ethanol from the product, separating glycerin, washing with water to obtain Acer truncatum Bunge oil, and measuring the nervonic acid content in the Acer truncatum Bunge oil by high performance liquid chromatography to obtain the Acer truncatum Bunge oil with 72.5%.
The procedure is as in example 2.
Comparative example 2
The preparation method of the dietary supplement in the comparative example comprises the following steps:
the procedure of example 2 was followed except that Acer truncatum oil was used as the starting material (1).
a. Acer truncatum seeds are taken as raw materials to obtain Acer truncatum oil through supercritical extraction;
b. absolute ethyl alcohol and acer truncatum oil are mixed according to the volume ratio of 1:4, after mixing, reacting for 16 hours at 50 ℃, removing unreacted ethanol from the product, separating glycerin, washing with water to obtain Acer truncatum oil, and measuring the nervonic acid content in the Acer truncatum oil by adopting a high performance liquid chromatography method to obtain 34.4 percent.
Comparative example 3
The preparation method of the dietary supplement in the comparative example comprises the following steps:
preparing a dietary supplement with the exception of (2), (4) and the same procedure as in example 2;
(2) Preparing liposome: a. dissolving 10g of soybean lecithin with purity of 98% and 4g of cholesterol in 100mL of absolute ethyl alcohol, and performing ultrasonic dissolution at a temperature below 0 ℃ to prepare a lipid solution;
(4) Preparing a dietary supplement: adding an emulsifying agent into the acer truncatum oil containing 65% -80% of nervonic acid and the hawthorn kernel essential oil respectively, and heating, wherein the mass ratio of the acer truncatum oil containing 65% -80% of nervonic acid to the hawthorn kernel essential oil is 4:1, stirring to obtain a milkshake-shaped mixture, slowly dripping the two mixtures into a fat solution, mixing, evaporating to remove ethanol, filtering with a filter membrane, drying at low temperature to obtain a product A, dispersing the product A into a sugar blocking solution, fully mixing, filtering with the filter membrane, and drying at low temperature to obtain a finished product, wherein the emulsifier is one of food-grade tween, span, monoglyceride or sucrose ester, and the emulsifier in the embodiment selects food-grade toast.
Comparative example 4
The preparation method of the dietary supplement in the comparative example comprises the following steps:
product a was taken as final product in step (4) with the exception of no step (3) as in example 2.
Comparative example 5
The preparation method of the dietary supplement in the comparative example comprises the following steps:
example 2 was repeated except for the step (4).
Adding chitosan modified liposome into absolute ethyl alcohol to obtain dispersion liquid, adding an emulsifying agent into the acer truncatum oil containing 65% -80% of nervonic acid, heating, stirring to form a milk shake-shaped mixture, slowly dripping into the dispersion liquid, mixing, evaporating to remove the ethanol, filtering by a filter membrane, drying at low temperature to obtain a product A, dispersing the product A into a sugar blocking solution, fully mixing, filtering by the filter membrane, and drying at low temperature to obtain a finished product, wherein the emulsifying agent is one of food-grade tween, span, monoglyceride or sucrose ester, and the emulsifying agent of the embodiment selects food-grade toast.
To verify the inclusion effect of the liposomes of the present application, the following experiments were performed:
50mg of the products A of examples 1-3 and comparative examples 1-5 were weighed respectively to prepare suspensions, and after at least 2 extractions, the concentration of the nervonic acid was measured by high performance liquid chromatography to calculate the inclusion rate.
The percentage inclusion rate can be calculated using the following formula:
inclusion% f /C t )×100％。
Wherein C is f In the amount of free nervonic acid, C t Is the total amount of nervonic acid in the liposome suspension.
TABLE 1 inclusion rate of product A
As can be seen from the table, the inclusion rate of the product A in the examples 1-3 is far greater than that in the comparative examples 1-5, the data in the examples and the changes in the comparative example 5 show that the hawthorn seed essential oil plays a certain role in the inclusion rate, and the coating effect of the liposome modified by chitosan is better than that of the liposome alone from the data in the comparative example 3.
To verify the lipid-lowering and blood glucose-lowering effects of the dietary supplements of the present application, the following tests were performed:
blood glucose lowering test
The dietary supplements prepared in example 2 and comparative examples 3-4 were intragastric fed to CD mice at 10% addition to common sweet potato starch, and postprandial glycemic changes in mice were measured against an equivalent amount of common sweet potato starch intragastric control mice, and mice taking the dietary supplements of the application were found to have significantly reduced postprandial glycemic responses. Further values of blood glucose concentration over time (blood obtained using the tail vein of the blood glucose meter) are shown in the following table:
TABLE 2 blood glucose reduction test results
Experiments prove that compared with the single use of common sweet potato starch, the dietary supplement provided by the application can well inhibit blood sugar after ingestion of carbohydrate and inhibit the abrupt rise of blood sugar concentration.
Lipid lowering test
Healthy Wistar rats of eight weeks of age were selected and fed continuously with high fat feed (composed of 60% fat, 40% normal feed formulation). 10 animals in each group are divided into 4 groups, wherein 3 groups are respectively fed with mixed feed of 5% of the dietary supplements prepared in the example 2 and the comparative examples 3 and 4 added in the high-fat feed, and the feeding amount is 5g/100g of the weight of the mice per day; the rest 1 group is continuously fed with high-fat feed, the feeding standard is the same as that of the rest 1 group, and the rest 1 group is continuously fed for 30 days. Average body weight gain for each group of rats is shown in the following table:
TABLE 3 lipid lowering test results
| Project | Weight/g before feeding | Weight/g after feeding | Weight gain/% |
| Example 2 | 102.3 | 122.4 | 19.6 |
| Comparative example 3 | 101.9 | 142.5 | 39.8 |
| Comparative example 4 | 102.2 | 143.2 | 40.1 |
| Blank space | 102.0 | 164.9 | 61.7 |
The results show that after 30 days, the body weight increase rate of rats taking the dietary supplement of the application is significantly lower than that of the blank group and comparative examples 3 and 4, and the difference is significant, which proves that the dietary supplement has an effect of effectively inhibiting fat absorption in diet, and the rats in the feeding group of example 2 are found to be more agile than those in the blank group when the physical ability test is performed on the rats in each group.
Example 5
The embodiment applies the dietary supplement rich in nervonic acid for promoting brain functions in the gel candy of the health care product, and the preparation method of the gel candy comprises the following steps:
(1) Adding 14.4% of glycerol, 34.2% of purified water, 15.2% of xylitol and 0.06% of stevioside into a gel dissolving tank according to mass percentage, uniformly stirring, heating to 60 ℃, adding 38.6% of gelatin, capping and sealing, stirring and heating to 75 ℃, stirring to form uniform glue solution, vacuumizing to remove bubbles in the glue solution, pressurizing to obtain glue, and sieving with a 100-mesh sieve to form capsule shell feed liquid;
(2) Adding the dietary supplement into DHA fish oil according to 1-2wt%, pouring into capsule shell liquid, drying, selecting pill, and removing unqualified pill such as abnormal pill and oil leakage pill to obtain gel candy.
Example 6
The embodiment applies the dietary supplement rich in nervonic acid for promoting brain functions in the gel candy of the health care product, and the preparation method of the gel candy comprises the following steps:
(1) Adding 14.8% of glycerol, 34.0% of purified water, 15.4% of xylitol and 0.03% of stevioside into a gel dissolving tank according to mass percentage, uniformly stirring, heating to 60 ℃, adding 36.2% of gelatin, capping and sealing, stirring and heating to 75 ℃, stirring to form uniform glue solution, vacuumizing to remove bubbles in the glue solution, pressurizing to obtain glue, and sieving with a 100-mesh sieve to form capsule shell feed liquid;
(2) Adding the dietary supplement into DHA fish oil according to 1-2wt%, pouring into capsule shell liquid, drying, selecting pill, and removing unqualified pill such as abnormal pill and oil leakage pill to obtain gel candy.
Comparative example 4
The comparative example applies the dietary supplement rich in nervonic acid for promoting brain function to the gel candy of the health care product, and the preparation method of the gel candy comprises the following steps:
(1) Adding 14.8% of glycerol, 34.3% of purified water and 15.7% of xylitol into a gelatin dissolving tank according to mass percentage, stirring uniformly, heating to 60 ℃, adding 38.6% of gelatin, capping and sealing, stirring and heating to 75 ℃, stirring to form uniform gelatin solution, vacuumizing to remove bubbles in the gelatin solution, pressurizing to obtain gelatin, and sieving with a 100-mesh sieve to form capsule shell feed liquid;
(2) Adding the dietary supplement into DHA fish oil according to 1-2wt%, pouring into capsule shell liquid, drying, selecting pill, and removing unqualified pill such as abnormal pill and oil leakage pill to obtain gel candy.
Melting temperatures of the gel candies of example 5, example 6 and comparative example 4 were measured and the results are shown in table 4 below:
| project | Example 5 | Example 6 | Comparative example 4 |
| Melting temperature/. Degree.C | 35.3 | 37.3 | 39.5 |
As can be seen from the table, the melting temperature of the gel candy in the embodiment 6 is more consistent with the human body temperature, the gel candy in the embodiment 5 is too low in temperature, is not easy to store, can be adhered for a long time, and is not beneficial to human body absorption because of too high melting temperature in the comparative embodiment 4.
The foregoing examples illustrate only a few embodiments of the application and are described in detail herein without thereby limiting the scope of the application. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the application, which are all within the scope of the application.
Claims (4)
1. A dietary supplement rich in nervonic acid for promoting cerebral functions is characterized in that acer truncatum oil containing 65% -80% of nervonic acid extracted from acer truncatum is taken as a main raw material, hawthorn kernel essential oil is taken as an auxiliary material, liposome inclusion modified by chitosan is carried out after emulsification by an emulsifying agent, and a mixture obtained by mixing sugar blocking solution is added;
the preparation method of the dietary supplement comprises the following steps:
(1) Acer truncatum seeds are taken as raw materials, and Acer truncatum grease containing 65-80% of nervonic acid is obtained through extraction, and the specific steps are as follows:
a. acer truncatum seeds are taken as raw materials to obtain Acer truncatum oil through supercritical extraction;
b. absolute ethyl alcohol and acer truncatum oil are mixed according to the volume ratio of 1: (1-10) adding 2-3wt% of D-sodium erythorbate into Acer truncatum oil after mixing, reacting for 1-5h at 50-80deg.C, removing unreacted ethanol from the product, separating glycerol, and washing with water to obtain Acer truncatum oil;
(2) Adding chitosan modified liposome into absolute ethyl alcohol to obtain dispersion liquid, respectively adding emulsifying agent into the acer truncatum oil containing 65% -80% of nervonic acid and the hawthorn kernel essential oil, heating, stirring to obtain milk shake-shaped mixture, slowly dripping the two mixtures into the dispersion liquid, mixing, evaporating to remove the ethyl alcohol, filtering by a filter membrane, and drying at low temperature to obtain a product A, wherein the mass ratio of the acer truncatum oil containing 65% -80% of nervonic acid to the hawthorn kernel essential oil is (2-5): 1, a step of;
the preparation method of the chitosan modified liposome comprises the following steps:
dissolving soybean lecithin and cholesterol in absolute ethyl alcohol, and performing ultrasonic dissolution at a temperature below 0 ℃ to prepare a lipid solution; dissolving chitosan in a mixed solution of acetic acid and phosphate buffer solution to obtain chitosan solution; finally, heating the lipid solution and the chitosan solution to 45-52 ℃, mixing, ultrasonically stirring, centrifuging and drying to obtain chitosan modified liposome;
(3) Dispersing the product A in a sugar blocking solution, fully mixing, filtering with a filter membrane, and drying at low temperature to obtain a finished product, wherein the preparation process of the sugar blocking solution comprises the following steps: dissolving conjugated linoleic acid to prepare a solution, heating to 50-60 ℃, and then respectively mixing hydroxycitric acid, a starch blocking agent and an o Luo Li sugar blocking agent according to the mass ratio of 1 (2-3): (1-2) adding the mixture to the solution after sufficient mixing to obtain a sugar-blocking solution.
2. The dietary supplement of claim 1, wherein the emulsifier in step (2) is one of food grade tween, span, monoglyceride or sucrose ester.
3. Use of a dietary supplement according to any one of claims 1-2 for promoting cerebral functioning enriched in nervonic acid in the preparation of a health product.
4. The use according to claim 3, wherein the health product is a gel candy, and the method for preparing the gel candy comprises the following steps:
(1) Adding 13-15% of glycerol, 30-35% of purified water, 14-16% of xylitol and 0.01-0.1% of stevioside into a gelatin dissolving tank according to mass percentage, uniformly stirring, heating to 60 ℃, adding 36-41% of gelatin, capping and sealing, stirring and heating to 75 ℃, stirring to form uniform gelatin solution, vacuumizing to remove bubbles in the gelatin solution, pressurizing to obtain gelatin, and sieving with a 100-mesh sieve to form capsule skin feed liquid;
(2) Adding the dietary supplement into DHA fish oil according to 1-2wt%, then pouring into the capsule shell feed liquid, drying, selecting pills, and removing unqualified pills such as abnormal pills, oil leakage pills and the like to obtain the gel candy.
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