CN114468194A - Preparation method of tricholoma matsutake active fungus sea cucumber health drink - Google Patents
Preparation method of tricholoma matsutake active fungus sea cucumber health drink Download PDFInfo
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
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- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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Abstract
A preparation method of a tricholoma matsutake active fungus sea cucumber health drink comprises the following steps: the method comprises the following steps: preparing a culture medium, wherein the components of the culture medium are as follows: adding 10-40g of corn flour and 10-25g of tricholoma matsutake powder into each liter of water; step two: preparing fermentation liquor, conventionally fermenting the culture medium, inoculating Tricholoma matsutake shake flask seeds, and performing fermentation culture, wherein the inoculation amount is 1-3% of the weight of the culture medium; step three: pulping the soaked sea cucumbers, filtering, and filtering with the filter mesh number of 400-600; step four: filtering and homogenizing, adding the prepared fermentation liquor and the sea cucumber slurry with the weight fraction of 2-5% of the fermentation liquor, pulping and filtering together, and homogenizing, wherein the filtering mesh number is 800 plus 1000 meshes. The preparation method of the tricholoma matsutake active fungus sea cucumber health drink adopts the tricholoma matsutake liquid fermentation technology, increases the nutrient components by adding the tricholoma matsutake powder and the epimedium into a submerged fermentation culture medium, improves the hypha content, and is rich in active hypha; the tricholoma matsutake fermentation liquid is added into the sea cucumber pulp, so that the nutrition of the beverage is further improved, and the fermentation medium has simple process and low cost.
Description
Technical Field
The invention relates to the technical field of beverage preparation.
Background
Matsutake, famous Tricholoma matsutake (lto et lmai) Singer, also known as matsutake mushroom, fungi, Taijun mushroom, belonging to Basidiomycotina, Tricholomataceae, is an ectotrophic mycorrhizal fungus of trees such as Quercus acutissima, and has unique and aromatic flavor. The tricholoma matsutake is a wild edible and medicinal fungus rich in various nutrient substances and functional active ingredients, is regarded as a treasure in the tricholoma matsutake, is really reputed by the "king of the tricholoma matsutake" and the "diamond in the mountain", but has a harsh growth environment, cannot realize complete artificial cultivation at present, has market demands mainly depending on wild resources, and has the advantages of reduced yield of the tricholoma matsutake, increasingly prominent contradiction between supply and demand in the market, increasing price of the tricholoma matsutake and great limitation on utilization and sustainable development of tricholoma matsutake resources due to changes of ecological environment, climatic conditions and unreasonable harvesting modes. For example, the matsutake can be used for soaking in wine to prepare health care wine, is a good health care drink, has good health care efficacy, medical efficacy and efficacy of improving human immunity, but the batch production is limited by resources. Therefore, the cost of the tricholoma matsutake is reduced, and the searching of the utilization mode of tricholoma matsutake resources is of great significance.
The tricholoma matsutake has bright color, brown pileus and white stipe, and all has fibrous hairy scales, white and tender mushroom flesh, thick texture and strong special fragrance. The tricholoma matsutake contains a large amount of active probiotics, and the active probiotics have the functions similar to SOD ferment in human body, can eliminate oxygen free radicals (free radicals), stop the source of diseases, delay cell aging, activate T cells, decompose nitroso compounds and other organic carcinogens, inhibit the growth of germs, viruses and cancer cells by the principle of benefiting bacteria and inhibiting bacteria, regulate the blood fat level of human body, reduce the fat deposition on the vessel wall, thereby recovering the natural self-healing power and enhancing and improving the immunologic function. However, people can not develop the best nutritional value by directly eating the middle-low-end tricholoma matsutake products when taking active probiotics.
Herba Epimedii is also called herba Epimedii leaf, herba Epimedii, and herba Epimedii. Epimedium herb, herba Epimedii, being pungent, sweet and warm in flavor, enters liver and kidney meridians. Has effects in resisting aging, lowering blood pressure, lowering blood sugar, and reducing blood lipid. Improving cerebral ischemia and anoxia, reducing blood viscosity, inhibiting in vitro thrombosis, stimulating synthesis of bone marrow DNA, increasing peripheral blood leukocyte and bone marrow hemopoietic stem cell number, enhancing immunity, promoting bone growth, preventing osteoporosis, relieving inflammation, resisting virus, resisting tumor, and resisting side effect of anticancer drugs. Meanwhile, has the effects of strengthening yang, dispelling wind and removing dampness and strengthening muscles and bones. Can be used for treating kidney essence deficiency, sexual impotence, spermatorrhea, premature ejaculation, cold sperm, infertility, pollakisuria, cough and asthma, soreness of waist and knees, spasm of muscles and bones, rheumatalgia, numbness, spasm, hemiplegia, numbness of limbs, climacteric hypertension, dribbling urination, cough and asthma.
Sea cucumber belongs to invertebrates, echinodermata and holothurians. Sea cucumber is distinguished as "eight delicacies" in the sea tastes of various mountain delicacies and also has the tonifying and health preserving functions of various traditional Chinese medicines. The study of more than 40 chemical components of sea cucumber by scholars at home and abroad for about half a century shows that the sea cucumber not only contains more than 50 nutrient components needed by human body such as amino acid, vitamin and chemical element, but also contains various bioactive substances such as acidic mucopolysaccharide, saponin and collagen, and the pharmacological activity of the active substances of the sea cucumber is very wide.
At present, no drink made of tricholoma matsutake is available in the market, the existing sea cucumber drink has single function and low nutrient content, and the technical problem to be solved by the invention is how to add the tricholoma matsutake into the sea cucumber drink and how to fully utilize the nutrition of the tricholoma matsutake.
Disclosure of Invention
The invention provides a preparation method of a tricholoma matsutake active fungus sea cucumber health drink, aiming at solving the problems that tricholoma matsutake ingredients are added into the sea cucumber drink, the full utilization of tricholoma matsutake nutrition can be realized, and the like.
The technical scheme adopted by the invention for realizing the purpose is as follows: a preparation method of a tricholoma matsutake active fungus sea cucumber health drink comprises the following steps:
the method comprises the following steps: preparing a culture medium, wherein the components of the culture medium are as follows: adding 10-40g of corn flour and 10-25g of tricholoma matsutake powder into each liter of water;
step two: preparing fermentation liquor, conventionally fermenting the culture medium, inoculating Tricholoma matsutake shake flask seeds, and performing fermentation culture, wherein the inoculation amount is 1-3% of the weight of the culture medium;
step three: pulping the soaked sea cucumbers, filtering, and filtering with the filter mesh number of 400-600;
step four: filtering and homogenizing, adding the prepared fermentation liquor and the sea cucumber slurry with the weight fraction of 2-5% of the fermentation liquor, pulping and filtering together, and homogenizing, wherein the filtering mesh number is 800 plus 1000 meshes.
The culture medium of the first step also comprises 5-20.75g of epimedium added into each liter of water.
In the second step, the preparation method of the tricholoma matsutake strain comprises the following steps:
(1) performing slant culture, taking the fruiting body of Tricholoma matsutake (lto et lmai) Singer whose mycoderm is not broken, sterilizing the surface of the fruiting body with 70% ethanol, taking the fruiting body off, inoculating into slant culture medium, sealing, placing into an incubator, culturing at constant temperature of 20-30 deg.C, and culturing in the next step after mycelia grow over the slant;
(2) and (2) liquid seed culture, namely inoculating the slant strain cultured in the step (1) into a shake flask corn powder liquid seed culture medium, and carrying out shake culture at the temperature of 20-30 ℃ and at the speed of 150-.
Further comprising the steps of:
step five: sterilizing and bottling, bottling the beverage at low temperature below 10 deg.C, and sealing the bottled beverage under aseptic condition;
step six: the taste and the effect are best when the beverage is refrigerated and preserved, and the beverage is refrigerated and preserved at the low temperature of 3-5 ℃ within 2-4 months.
In the first step, preferably, the culture medium contains 30g/L of corn flour, 15g/L of tricholoma matsutake powder and 15g/L of epimedium herb.
In the second step, preferably, the fermentation culture inoculation amount is 2%.
The step three, preferably, the filtration homogenization filtration mesh number is 1000 meshes.
The preparation method of the tricholoma matsutake active fungus sea cucumber health drink adopts the tricholoma matsutake liquid fermentation technology, increases the nutrient components by adding the tricholoma matsutake powder and the epimedium into a submerged fermentation culture medium, improves the hypha content, and is rich in active hypha; the tricholoma matsutake fermentation liquid is added into the sea cucumber pulp, so that the nutrition of the beverage is further improved, and the fermentation medium has simple process and low cost.
Detailed Description
Embodiment 1, a method for preparing a sea cucumber health drink by using tricholoma matsutake active bacteria comprises the following steps:
firstly, slant culture: taking the tricholoma matsutake fruiting body without rupture of the mycoderm, repeatedly carrying out surface sterilization by using 70% ethanol in parts by mass, taking off a small piece by using sterile forceps and a knife, inoculating the small piece into a slant culture medium, sealing, putting into an incubator, culturing at a constant temperature of 25 ℃, and transferring to the next step for culturing after hyphae grow over the slant;
secondly, liquid seed culture: inoculating the slant strain cultured in the step one into a shake flask corn flour liquid seed culture medium according to a conventional process, and carrying out shake culture at 25 ℃ and 190r/min for 8 days to obtain a liquid shake flask strain;
thirdly, fermentation culture:
1. sterilizing a fermentation tank at high pressure, and mixing 30g of corn flour, 15g of tricholoma matsutake powder, 15g of epimedium and 1 liter of water under aseptic condition for sterilization for later use;
2. inoculating shake flask strains, and performing fermentation culture, wherein the inoculation amount is 3% of the weight of the culture medium;
3. conventionally culturing to prepare fermentation liquor;
pulping the sea cucumber, namely cleaning, removing impurities, pulping and filtering the fresh sea cucumber or the soaked sea cucumber, and filtering the pulp with the mesh number of 600;
fifthly, filtering and homogenizing, adding the prepared fermentation liquor and the sea cucumber slurry with the mass fraction of 2-5%, pulping and filtering together, and homogenizing, wherein the filtering mesh number is 1000 meshes;
and sixthly, sterilizing and bottling, bottling the beverage at the low temperature of below 10 ℃, and sealing the bottled beverage in an aseptic state.
Sixthly, refrigerating and preserving, wherein the drinking taste and the effect are optimal within 3 months of refrigerating and preserving at the low temperature of 3-5 ℃.
The health-care beverage prepared by the steps has better taste and is rich in high-nutrition components.
Embodiment 2, a method for preparing a sea cucumber health drink by using tricholoma matsutake active bacteria, comprising the following steps:
firstly, slant culture: taking the tricholoma matsutake fruiting body without rupture of the mycoderm, repeatedly carrying out surface sterilization by using 70% ethanol in parts by mass, taking off a small piece by using sterile forceps and a knife, inoculating the small piece into a slant culture medium, sealing, putting into an incubator, culturing at a constant temperature of 25 ℃, and transferring to the next step for culturing after hyphae grow over the slant;
secondly, liquid seed culture: and (3) inoculating the slant strain cultured in the step one into a shake flask corn flour liquid seed culture medium according to a conventional process, and carrying out shake culture at 25 ℃ and 190r/min for 8 days to obtain a liquid shake flask strain.
Thirdly, fermentation culture:
1. sterilizing a fermentation tank at high pressure, adding 40g/L corn flour, 10-g/L pine mushroom powder and 20.g/L epimedium into each liter of water under aseptic condition, mixing and sterilizing for later use;
2. inoculating shake flask strains, and performing fermentation culture, wherein the inoculation amount is 1% of the weight of the culture medium;
3. conventionally culturing to prepare fermentation liquor;
pulping the sea cucumbers, namely cleaning, removing impurities, pulping and filtering the live and foamed sea cucumbers, and filtering the pulp to obtain 500 meshes;
fifthly, filtering and homogenizing, adding 2-5% of the sea cucumber slurry into the prepared fermentation liquor, pulping and filtering together, homogenizing, and filtering with 800 meshes;
sixthly, sterilizing and bottling; bottling the beverage at a low temperature below 15 deg.C, and sealing the bottled beverage under aseptic condition.
Sixthly, the method comprises the following steps: refrigerating and preserving; the mouthfeel and the effect are optimal when the beverage is refrigerated and preserved at the low temperature of 3-5 ℃ within 2 months.
Experiments prove that the mycelium content in the culture medium added with the epimedium is far higher than that in the culture medium not added with the epimedium. The health-care beverage prepared by the steps has better taste and is rich in high-nutrition components.
The mycelium dry substance produced by the production process for producing the tricholoma matsutake mycelium through fermentation has the highest fermentation yield of more than 25g/L, and the mycelium dry substance yield in the prior art is only about 18g/L, so the production process is remarkably improved compared with the production process of the mycelium dry substance in the prior art, the production process is suitable for the fermentation culture of tricholoma matsutake, and the mycelium dry substance yield is stable and the yield is high.
The present invention has been described in terms of embodiments, and it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the spirit and scope of the invention. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the invention without departing from the essential scope thereof. Therefore, it is intended that the invention not be limited to the particular embodiment disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.
Claims (7)
1. A preparation method of a tricholoma matsutake active fungus sea cucumber health care beverage is characterized by comprising the following steps: the method comprises the following steps:
the method comprises the following steps: preparing a culture medium, wherein the components of the culture medium are as follows: adding 10-40g of corn flour and 10-25g of tricholoma matsutake powder into each liter of water;
step two: preparing fermentation liquor, conventionally fermenting the culture medium, inoculating Tricholoma matsutake shake flask seeds, and performing fermentation culture, wherein the inoculation amount is 1-3% of the weight of the culture medium;
step three: pulping the soaked sea cucumbers, filtering, and filtering with the mesh number of 400-600;
step four: filtering and homogenizing, adding the prepared fermentation liquor and the sea cucumber slurry with the weight fraction of 2-5% of the fermentation liquor, pulping and filtering together, and homogenizing, wherein the filtering mesh number is 800 plus 1000 meshes.
2. The method for preparing the tricholoma matsutake active fungus and sea cucumber health drink according to claim 1 is characterized in that: the culture medium of the first step also comprises 5-20.75g of epimedium added into each liter of water.
3. The method for preparing the tricholoma matsutake active fungus and sea cucumber health drink according to claim 2 is characterized in that: in the second step, the preparation method of the tricholoma matsutake strain comprises the following steps:
(1) performing slant culture, taking the fruiting body of Tricholoma matsutake (lto et lmai) Singer whose mycoderm is not broken, sterilizing the surface of the fruiting body with 70% ethanol, taking the fruiting body off, inoculating into slant culture medium, sealing, placing into an incubator, culturing at constant temperature of 20-30 deg.C, and culturing in the next step after mycelia grow over the slant;
(2) and (2) liquid seed culture, namely inoculating the slant strain cultured in the step (1) into a shake flask corn powder liquid seed culture medium, and carrying out shake culture at the temperature of 20-30 ℃ and at the speed of 150-.
4. The method for preparing the tricholoma matsutake active fungus and sea cucumber health drink according to claim 1 is characterized in that: further comprising the steps of:
step five: sterilizing and bottling, bottling the beverage at low temperature below 10 deg.C, and sealing the bottled beverage under aseptic condition;
step six: the taste and the effect are best when the beverage is refrigerated and preserved, and the beverage is refrigerated and preserved at the low temperature of 3-5 ℃ within 2-4 months.
5. The method for preparing the tricholoma matsutake active fungus and sea cucumber health drink according to claim 2 is characterized in that: in the first step, preferably, the culture medium contains 30g/L of corn flour, 15g/L of tricholoma matsutake powder and 15g/L of epimedium herb.
6. The method for preparing the tricholoma matsutake active fungus and sea cucumber health drink according to claim 1 is characterized in that: in the second step, preferably, the fermentation culture inoculation amount is 2%.
7. The method for preparing the tricholoma matsutake active fungus sea cucumber health care beverage according to claim 1, which is characterized by comprising the following steps: the step three, preferably, the filtration homogeneous filtration mesh number is 1000 meshes.
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| CN102771857A (en) * | 2012-07-20 | 2012-11-14 | 黄晓青 | Truffle health-care drink prepared through liquid submerged fermentation and preparation method of truffle health-care drink prepared through liquid submerged fermentation |
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| CN110199778A (en) * | 2019-06-04 | 2019-09-06 | 临沂信邦生物科技有限公司 | A kind of production technology using fermentation of corn starch production tricholoma matsutake mycelium |
| CN111919986A (en) * | 2020-07-29 | 2020-11-13 | 杨玉波 | Method for preparing health beverage from active bacteria extracted from Tricholoma matsutake |
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| CN102771857A (en) * | 2012-07-20 | 2012-11-14 | 黄晓青 | Truffle health-care drink prepared through liquid submerged fermentation and preparation method of truffle health-care drink prepared through liquid submerged fermentation |
| CN104068418A (en) * | 2013-03-28 | 2014-10-01 | 威海健方医药研究所 | Production method for trepang beverage enhancing immunity |
| CN105077365A (en) * | 2015-07-21 | 2015-11-25 | 刘海涛 | A food formula of delicious precious fungus stewed sea cucumber |
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