CN114460071A - Test paper structure for detecting carcinogenic substances in food - Google Patents

Test paper structure for detecting carcinogenic substances in food Download PDF

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Publication number
CN114460071A
CN114460071A CN202210056396.6A CN202210056396A CN114460071A CN 114460071 A CN114460071 A CN 114460071A CN 202210056396 A CN202210056396 A CN 202210056396A CN 114460071 A CN114460071 A CN 114460071A
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Prior art keywords
test paper
detection
cover plate
chassis
detecting
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Withdrawn
Application number
CN202210056396.6A
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Chinese (zh)
Inventor
程庆
江超
付收硕
欧峥嵘
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Xiangnan University
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Xiangnan University
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Priority to CN202210056396.6A priority Critical patent/CN114460071A/en
Publication of CN114460071A publication Critical patent/CN114460071A/en
Withdrawn legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/15—Preventing contamination of the components of the optical system or obstruction of the light path
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/04—Batch operation; multisample devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/04—Batch operation; multisample devices
    • G01N2201/0446—Multicell plate, sequential

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plasma & Fusion (AREA)
  • Nanotechnology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The invention discloses a test paper structure for detecting carcinogenic substances in food, which comprises a base plate, wherein a plurality of cutting grooves are formed on the base plate along the circumferential direction; a cover plate is arranged above the chassis, a plurality of slices corresponding to the cutting grooves are formed on the cover plate in a protruding mode along the circumferential direction, and detection holes are formed between every two adjacent slices; an annular test paper body is arranged between the base plate and the cover plate; the test paper body is cut into a plurality of test paper blocks corresponding to the detection holes through a cutting groove and a slicing; the outer diameter of the base plate is larger than that of the cover plate; the edge of the chassis is stuck with a color comparison card. The invention can rapidly cut a test paper into a plurality of small pieces, thereby realizing the full utilization of the test paper, realizing the repeated detection of harmful substances and realizing the detection of various harmful substances.

Description

Test paper structure for detecting carcinogenic substances in food
Technical Field
The invention relates to the field of machinery, in particular to a test paper structure for detecting carcinogenic substances in food.
Background
The test paper is a detection article for detecting whether a species to be detected contains a target substance, and the compound contained in the long test paper can react with the target substance to generate a color reaction, and the concentration of the target substance can be approximately obtained according to the color type. When the food is prepared, lawless persons can add excessive food additives, and harmful substances such as mildew and the like can be generated when the food is stored. Therefore, when detecting food, different test paper is often used to detect whether harmful substances exist in the food and the content of the harmful substances. However, the existing test paper is usually strip-shaped, when in detection, people crush food, then add a certain amount of water to dissolve the food, hold one end of the test paper by hand, stretch the other end of the test paper into a solution, then take out the test paper, and carry out colorimetric detection. Some can adopt the detection card, be about to a test paper centre gripping between two boards, one of them board opening, the measuring chamber absorbs solution dropwise to the test paper, then the color comparison, but this kind of detection card can only use once, also can cause the waste of resource.
In addition, the existing detection card can only detect one harmful substance in turn, which causes inconvenience in use.
Disclosure of Invention
In order to solve the technical problem, the invention provides a test paper structure for detecting carcinogenic substances in food. The invention can rapidly cut a test paper into a plurality of small pieces, thereby realizing the full utilization of the test paper, realizing the repeated detection of harmful substances and realizing the detection of various harmful substances.
The purpose of the invention is realized by the following technical scheme:
a test paper structure for detecting carcinogenic substances in food comprises a base plate, wherein a plurality of cutting grooves are formed on the base plate along the circumferential direction; a cover plate is arranged above the chassis, a plurality of slices corresponding to the cutting grooves are formed on the cover plate in a protruding mode along the circumferential direction, and detection holes are formed between every two adjacent slices; an annular test paper body is arranged between the chassis and the cover plate; the test paper body is cut into a plurality of test paper blocks corresponding to the detection holes through a cutting groove and a slicing; the outer diameter of the base plate is larger than that of the cover plate; the edge of the chassis is stuck with a color comparison card.
The test paper body sequentially comprises an aflatoxin detection functional area, a formaldehyde detection functional area, a nitrite detection functional area and a benzopyrene detection functional area along the circumferential direction.
In a further improvement, a first notch is formed on the test paper body, and a second notch corresponding to the first notch is formed on the chassis; corresponding comparison color cards are respectively stuck on the edge of the chassis corresponding to the aflatoxin detection functional area, the formaldehyde detection functional area, the nitrite detection functional area and the benzopyrene detection functional area.
In a further improvement, the cover plate is provided with an initial detection port mark and an end detection port mark which respectively correspond to the aflatoxin detection functional area, the formaldehyde detection functional area, the nitrite detection functional area and the benzopyrene detection functional area.
In a further improvement, a rotating shaft penetrates through the middle parts of the base plate and the cover plate, a transparent dustproof plate is connected to the rotating shaft in a shaft mode, and a through hole or four through holes corresponding to the detection holes are formed in the transparent dustproof plate.
In a further improvement, a plurality of spherical clamping grooves are formed in the inner side of the cover plate along the circumferential direction, grooves corresponding to the spherical clamping grooves are formed in the rotating shaft, springs are installed in the grooves, and clamping balls are installed between the springs and the spherical clamping grooves.
In a further improvement, the rotating shaft is connected with the transparent dust guard in a shaft connection mode through a bearing.
In a further improvement, a plurality of radial rotary convex ribs are formed on the transparent dustproof plate in a protruding mode.
In a further improvement, a counter bore is formed at the bottom of the chassis, and a fixing bolt penetrates through the counter bore to be in threaded connection with the rotating shaft.
The invention has the beneficial effects that:
the invention can rapidly cut a test paper into a plurality of small pieces, thereby realizing the full utilization of the test paper, realizing the repeated detection of harmful substances and realizing the detection of various harmful substances.
Drawings
The invention is further illustrated by means of the attached drawings, the content of which is not in any way limitative of the invention.
FIG. 1 is a schematic cross-sectional view of the present invention;
FIG. 2 is a schematic top view of the present invention;
FIG. 3 is a schematic top view of the base pan;
FIG. 4 is a bottom view of the cover plate;
FIG. 5 is a schematic structural diagram of the test strip body.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings and examples.
Example 1
The test paper structure for detecting carcinogenic substances in food as shown in figure 1 comprises a chassis 1, wherein a plurality of cutting grooves 2 are formed on the chassis 1 along the circumferential direction; a cover plate 3 is arranged above the chassis 1, a plurality of slices 4 corresponding to the cutting grooves 2 are convexly formed on the cover plate 3 along the circumferential direction, and a detection hole 5 is formed between every two adjacent slices 4; an annular test paper body 6 is arranged between the chassis 1 and the cover plate 3; the test paper body 6 is cut into a plurality of test paper blocks corresponding to the detection holes 5 through the cutting grooves 2 and the cutting sheets 4; the outer diameter of the chassis 1 is larger than that of the cover plate 3; a color comparison card 7 is stuck on the edge of the chassis 1.
The cover plate 3 is formed with a start detection port mark 18 and a tail detection port mark 19
The middle parts of the chassis 1 and the cover plate 3 are penetrated by a rotating shaft 10, the rotating shaft 10 is connected with a transparent dustproof plate 11 in a shaft connection mode, and a through hole 12 corresponding to the detection hole 5 is formed in the transparent dustproof plate 11.
A plurality of spherical clamping grooves 13 are formed in the inner side of the cover plate 3 along the circumferential direction, grooves 14 corresponding to the spherical clamping grooves 13 are formed in the rotating shaft 10, springs 15 are installed in the grooves 14, and clamping balls 16 are installed between the springs 15 and the spherical clamping grooves 13.
The rotating shaft 10 is connected with the transparent dust-proof plate 11 through a bearing 17 in a shaft coupling mode.
The transparent dust-proof plate 11 is formed with a plurality of radial rotary convex ribs 20.
A counter bore 21 is formed at the bottom of the chassis 1, and a fixing bolt 22 penetrates through the counter bore 21 to be in threaded connection with the rotating shaft 10.
The using method of the test paper comprises the steps of firstly placing the test paper body 6 on the base plate 1, then covering the cover plate 3, aligning the section 4 of the cover plate with the cutting groove 2, then pressing downwards, and cutting the annular test paper body 6 into a plurality of unconnected segments by the section 4. Then, the transparent dust-proof plate 11 is covered, and the rotating shaft 10 coupled to the transparent dust-proof plate 11 is passed through the chassis 1 and the cover plate 3 and then the fixing bolt 22 is coupled, so that the chassis 1, the cover plate 3 and the transparent dust-proof plate 11 are coupled together. And make the through-hole 12 aim at the 18 departments of originated detection mouth sign, when carrying out the food detection, will wait to detect a material and instil into the inspection hole 5 that through-hole 12 corresponds, then rotatory transparent dust guard 11, during the rotation, transparent dust guard 11 compression card pearl 16, when through-hole 12 aligns with next inspection hole 5, the card pearl is popped out by the spring and just goes into spherical draw-in groove 13, instil into corresponding inspection hole 5 with the material of next part can. The sample invention can realize the detection of a plurality of samples. When all the detection holes 5 are used, namely the through holes 12 are positioned at the tail detection port mark 19, the fixing bolts 22 are unscrewed, all the used test paper is taken out, and the new test paper is replaced.
Example 2
As shown in fig. 2 to 5, in order to detect a plurality of different harmful substances simultaneously, the test strip body 6 sequentially includes an aflatoxin detection functional region 61, a formaldehyde detection functional region 62, a nitrite detection functional region 63, and a benzopyrene detection functional region 64 along the circumferential direction. Corresponding comparison color cards 7 are respectively stuck on the edge of the chassis 1 corresponding to the aflatoxin detection functional area 61, the formaldehyde detection functional area 62, the nitrite detection functional area 63 and the benzopyrene detection functional area 64. A first notch 8 is formed on the test paper body 6, and a second notch 9 corresponding to the first notch 8 is formed on the chassis 1.
The cover plate 3 is formed with a start detection port mark 18 and an end detection port mark 19 which respectively correspond to the aflatoxin detection functional zone 61, the formaldehyde detection functional zone 62, the nitrite detection functional zone 63 and the benzopyrene detection functional zone 64. The test paper body 6 can be four arc-shaped functional areas which are respectively arranged, and the four functional areas are bonded together to form an annular integral structure. The transparent dust-proof plate 11 is formed with a through hole 12 corresponding to each functional region.
The test paper is divided into four different functional areas, each functional area detects a different carcinogenic substance, and different detection principles are adopted according to the physicochemical properties and biochemical characteristics of the different carcinogenic substances:
the aflatoxin (double antibody sandwich method) is based on specific antigen-antibody reaction and immunochromatography, and an antibody and an enzyme-labeled antibody which are connected to a solid phase carrier are respectively combined with two antigenic determinants on a detected antigen molecule (aflatoxin B1) in a sample to form a solid phase antibody-antigen-enzyme-labeled antibody immune complex; otherwise, the detection color band does not develop color, and the result is negative.
Formaldehyde (formaldehyde residue test paper) is prepared by using a principle that fuchsin reacts with sulfurous acid to generate a colorless Schiff reagent, and reacts with formaldehyde to generate a mauve compound under room temperature and acidic conditions, soaking filter paper by using a mixed solution of the Schiff reagent, ethylenediamine, disodium tetraacetate and citric acid, and drying to obtain the rose-red formaldehyde residue test paper. And (3) preparing standard formaldehyde solutions with different contents, dripping the standard formaldehyde solutions on test paper, and observing the color development condition.
Under specific conditions, the nitrite (chemical color development method) performs color development reaction with a specific chemical reagent on the test paper, the color depth of the nitrite is in direct proportion to the content of nitrite ions, and the qualitative or semi-quantitative analysis of the nitrite is realized by comparing the color development result with a standard color comparison card.
Benzopyrene (immune colloidal gold layer technology) firstly synthesizes benzopyrene hapten, and the benzopyrene hapten is coupled with carrier protein to obtain immune antigen. Then, the monoclonal antibody is successfully obtained by utilizing an artificial immunization method, and the performance of the prepared monoclonal antibody is verified by establishing an enzyme-linked immunosorbent assay method. The monoclonal antibody is used for marking colloidal gold, and benzopyrene coated antigen and a second antibody (goat anti-mouse IgG) are respectively sprayed on a nitrocellulose membrane, and are sequentially adhered to a PVC lining plate together with a sample pad and a water absorption pad to prepare the colloidal gold chromatography test strip.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims (9)

1. A test paper structure for detecting carcinogenic substances in food is characterized by comprising a base plate (1), wherein a plurality of cutting grooves (2) are formed on the base plate (1) along the circumferential direction; a cover plate (3) is arranged above the chassis (1), a plurality of slices (4) corresponding to the cutting grooves (2) are formed in the cover plate (3) in a protruding mode along the circumferential direction, and a detection hole (5) is formed between every two adjacent slices (4); an annular test paper body (6) is arranged between the chassis (1) and the cover plate (3); the test paper body (6) is cut into a plurality of test paper blocks corresponding to the detection holes (5) through the cutting grooves (2) and the slices (4); the outer diameter of the chassis (1) is larger than that of the cover plate (3); a color comparison card (7) is stuck on the edge of the chassis (1).
2. The test paper structure for detecting carcinogenic substances in food according to claim 1, wherein the test paper body (6) sequentially comprises an aflatoxin detection functional zone (61), a formaldehyde detection functional zone (62), a nitrite detection functional zone (63) and a benzopyrene detection functional zone (64) along the circumferential direction.
3. The test paper structure for detecting carcinogenic substances in food according to claim 2, wherein a first notch (8) is formed on the test paper body (6), and a second notch (9) corresponding to the first notch (8) is formed on the chassis (1); the corresponding comparison color cards (7) are respectively stuck to the edge of the chassis (1) corresponding to the aflatoxin detection functional area (61), the formaldehyde detection functional area (62), the nitrite detection functional area (63) and the benzopyrene detection functional area (64).
4. The structure of the test paper for detecting carcinogenic substances in food according to claim 2, wherein the cover plate (3) is formed with a start detection port mark (18) and an end detection port mark (19) corresponding to the aflatoxin detection functional region (61), the formaldehyde detection functional region (62), the nitrite detection functional region (63), and the benzopyrene detection functional region (64), respectively.
5. The structure of the test paper for detecting the carcinogenic substances in the food according to claim 1 or 2, wherein a rotating shaft (10) penetrates through the middle of the base plate (1) and the cover plate (3), a transparent dust-proof plate (11) is coupled on the rotating shaft (10), and a through hole (12) or four through holes (12) corresponding to the detection hole (5) are formed in the transparent dust-proof plate (11).
6. The structure of the test paper for detecting the carcinogenic substances in the food according to claim 5, wherein a plurality of spherical clamping grooves (13) are formed on the inner side of the cover plate (3) along the circumferential direction, grooves (14) corresponding to the spherical clamping grooves (13) are formed on the rotating shaft (10), springs (15) are installed in the grooves (14), and clamping beads (16) are installed between the springs (15) and the spherical clamping grooves (13).
7. The structure of the test paper for detecting carcinogenic substances in food according to claim 5, wherein said rotating shaft (10) is connected with the transparent dust-proof plate (11) by a shaft bearing (17).
8. The structure of the test paper for detecting the carcinogenic substances in the food according to claim 5, wherein a plurality of radial rotating ribs (20) are convexly formed on the transparent dust-proof plate (11).
9. The structure of the test paper for detecting the carcinogenic substances in the food according to claim 5, wherein a counter bore (21) is formed at the bottom of the chassis (1), and a fixing bolt (22) passes through the counter bore (21) and is in threaded connection with the rotating shaft (10).
CN202210056396.6A 2022-01-18 2022-01-18 Test paper structure for detecting carcinogenic substances in food Withdrawn CN114460071A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210056396.6A CN114460071A (en) 2022-01-18 2022-01-18 Test paper structure for detecting carcinogenic substances in food

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210056396.6A CN114460071A (en) 2022-01-18 2022-01-18 Test paper structure for detecting carcinogenic substances in food

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CN114460071A true CN114460071A (en) 2022-05-10

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Application publication date: 20220510