CN114431224B - Connecting part suitable for connecting glass pasteur suction tube and liquid transfer device - Google Patents
Connecting part suitable for connecting glass pasteur suction tube and liquid transfer device Download PDFInfo
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- CN114431224B CN114431224B CN202210227008.6A CN202210227008A CN114431224B CN 114431224 B CN114431224 B CN 114431224B CN 202210227008 A CN202210227008 A CN 202210227008A CN 114431224 B CN114431224 B CN 114431224B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
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Abstract
The invention provides a liquid transfer device with a connecting component for connecting a glass pasteur pipette, which comprises a liquid transfer control component and a liquid transfer tube, wherein the liquid transfer control component comprises a sample loading display interface, a washing display interface, a charging interface, an oil filling port and a rotary knob, the rotary knob pushes oil to move through the rotary knob, and the front end of the glass pasteur pipette is controlled by oil pressure to quantitatively suck or discharge oocytes or embryos containing cryoprotectants; the connecting member includes: the glass Pasteur pipette comprises a suction head, a glass Pasteur pipette, a plurality of spiral blades, a ring-shaped sealing gasket, a liquid transfer pipe and a connecting component, wherein the spiral blades are used for being inserted into an opening of the glass Pasteur pipette, the spiral blades are arranged in an array mode, when the glass Pasteur pipette is screwed into the opening, the end, far away from the suction head, of the glass Pasteur pipette is sealed with the liquid transfer pipe of a liquid transfer machine, and the spiral blades press the outer surface of the glass Pasteur pipette to enable the connecting component to be tightly combined with the outer surface of the glass Pasteur pipette.
Description
Technical Field
The invention relates to the technical field of pipettors, in particular to a connecting part suitable for connecting a glass Pasteur suction pipe and a pipettor.
Background
The cryopreservation of the oocytes and the embryos is a complex process, the bioactivity of the oocytes and the embryos is reduced at low temperature, the bioactivity is almost completely stopped when the temperature reaches-196 ℃, the purpose of long-time preservation is achieved, and the cells are required to keep complete structure and normal functions after being frozen and thawed and can normally develop after being placed back in a normal physiological environment. The freezing methods are classified according to freezing speed: 1 slow freeze/fast thaw: 0.5-1.5M of cryoprotectant is adopted, and the freezing speed is 0.3-2.0 ℃/min; 2 quick freezing/quick thawing: 5-8M of freezing protective agent is adopted, and the freezing speed is 500 ℃/min; 3 ultrafast freezing (vitrification freezing): the freezing and thawing speed must be greater than 2000 deg.c/min.
The concentration of the cryoprotectant in the freezing process has great influence on the subsequent development potential of the oocyte or embryo, particularly the high-concentration cryoprotectant has great influence on the subsequent development, and in practice, the cryoprotectant with high concentration is abandoned, and slow freezing and ultra-fast freezing, namely the conventional programmed freezing method and vitrification freezing method are used instead of fast freezing.
The damage to cells in the freezing process mainly has two aspects, one is damage of a cryoprotectant, the cryoprotectant can cause toxic damage to the cells, and the rapid inlet and outlet of the cryoprotectant can form osmotic pressure impact on cell membranes to influence the functions of the cell membranes. The second is damage by ice crystals, which can disrupt the membrane structure of the cell, causing functional damage or directly leading to cell death. Programmed freezing is to replace water in cells by using a cryoprotectant, then slowly cool the cells, form small ice crystals in the cells, and artificially plant ice at-7 ℃ to avoid forming large ice crystals to damage the cells. In 1972 Mazur proposed two-factor hypothesis of freezing damage, one was the formation of ice crystals by over-rapid freezing, the more damage at the faster freezing rate, and the other was solution damage, the more damage the longer the cells were exposed to high concentration of liquid (the slower the freezing rate, the greater the damage).
Vitrification refers to the formation of vitrification when a substance is at a temperature less than or equal to the "glass transition temperature" of the substance, for example, water forms vitrification at a sufficient rate of temperature reduction and the temperature is reduced to-130 ℃. During freezing, the temperature is rapidly reduced, and the liquid in the cell does not form ice crystals but is in a glassy state between solid and liquid. The freeze recovery rate of embryo programmed freezing in the cleavage stage of the third day is continuously improved and is maintained to be more than 70 percent at present. The recovery rate of vitrification embryo freezing can reach more than 90%, so vitrification freezing is the freezing method mainly used clinically at present.
The vitrification freezing technology is a method combining quick freezing and high-concentration cryoprotectant. The rapid freezing forms transparent glassy solid at low temperature, the viscosity is very high in the cooling process, but no ice crystal is formed, the damage of the ice crystal form to the cell lipid membrane and the cell skeleton structure is avoided, and the normal molecular and ion distribution of liquid inside and outside the cell is reserved. The main operations of vitrification freezing include replacing the solution inside and outside cell with high concentration cryoprotectant, fast cooling to raise the viscosity of the solution, solidification and preservation in liquid nitrogen.
Two factors of vitrification are solution concentration and freezing rate, vitrification is more likely to occur as the solution concentration is higher, and vitrification is more likely to occur as the temperature decrease rate is higher, but the freezing rate needs to be increased as much as possible because the cryoprotectant concentration is high and has a toxic effect on embryos. There are two methods for increasing the freezing rate, one is to reduce the volume of the liquid to be frozen, the smaller the volume, the faster the freezing rate; another is to increase the temperature difference between the room temperature and the freezing medium, the greater the temperature difference the higher the freezing rate.
Currently, the method commonly used for freezing oocytes or embryos is the vitrification freezing method. The vitrification freezing operation steps are simple, and include replacing the solution inside and outside the cell with high concentration cryoprotectant, fast cooling to raise the viscosity of the solution and solidify, and storing in liquid nitrogen.
An important operational step in achieving rapid cooling is the need to load as little volume as possible of the cryoprotectant containing the oocyte or embryo onto the cryo-loading rod. This operation cannot be standardized between operators, and between different operations of the same operator, i.e. the freezing operation to load the embryos onto the loading rods is not quality control standard.
The existing method for aspiration of oocytes or embryos is to use a thinned glass pasteur pipette and manually press a plastic tip at the tip of the pipette, thereby controlling the amount of liquid aspirated into the glass pasteur pipette. The oocyte or embryo containing the cryoprotectant can be manually controlled to be sucked into a glass Pasteur pipette and then be discharged onto a freezing carrying rod, but the existing method cannot quantify, the blowing and the sucking are laborious, a large amount of training is needed, and the cost is consumed.
Currently, in the freezing process of oocytes or embryos, the following problems exist:
1. when the oocytes or embryos are subjected to cryopreservation, the size of the oocytes or embryos loaded with cryoprotectants is required to be as small as possible when the oocytes or embryos are loaded on the freezing loading rod, and the thin glass pasteur pipette is manually controlled to complete the loading process, so that no specific standard exists, human errors exist when different operators perform freezing operation, and the freezing effect cannot be subjected to quality control in the step.
2. When the same operator carries out a plurality of times of cryopreservation operations and loads oocytes or embryos onto the freezing carrying rod, the standardization of the dosage of the cryoprotectant cannot be controlled.
3. In the prior art, a manual or electric pipette is a tool used in molecular biology, and a plastic sucker is used. But plastic tips are not adequate for freezing operations. The reason is that: first, untreated plastic pipettes are toxic to oocytes or embryos and cannot be used; secondly, the tube wall of the plastic suction head is not smooth, so that the oocyte or embryo can be adsorbed on the tube wall of the suction head and cannot be washed off; thirdly, the plastic suction head is not transparent, so that the position of the sucked oocyte or embryo cannot be accurately grasped, and therefore, whether the embryo is completely loaded or not or is adhered to the plastic suction head cannot be controlled when the embryo is loaded on the freezing loading rod.
In current clinical practice, oocyte or embryo transfer is usually performed by means of a sterilized glass pasteur pipette tip. However, the prior art pipette is not directly connected to the glass pasteur pipette.
4. Oocyte or embryo freezing procedures require significant training by the operator, take significant training time and expense, and are cost prohibitive.
Therefore, in order to solve the problem that the glass pasteur pipette cannot be directly connected with a pipette in the prior art to load the oocyte or embryo on the freezing carrying rod, a connecting component suitable for connecting the glass pasteur pipette and the pipette are needed.
Disclosure of Invention
The invention provides a connecting part suitable for connecting a glass pasteur pipette and a pipette, and aims to solve the technical problem that the standardization of the sample adding amount of a cryoprotectant cannot be controlled when the glass pasteur pipette is used for loading oocytes or embryos onto a freezing carrying rod in the prior art.
One aspect of the present invention is to provide a connecting member suitable for connecting glass pasteur pipettes, comprising:
an opening for inserting a glass pasteur pipette,
in the opening, a plurality of helical blades having elasticity are arrayed,
an annular sealing gasket, when the glass Pasteur pipette is screwed into the opening, the end part of the glass Pasteur pipette far away from the suction head is sealed with a pipette of the pipettor through the annular sealing gasket,
and the helical blade presses the outer surface of the glass pasteur pipette to tightly combine the connecting component with the outer surface of the glass pasteur pipette.
In a preferred embodiment, the connecting part has a conical structure and further comprises a socket for inserting a pipette of a pipette.
In a preferred embodiment, the connecting part is a hollow cylindrical structure, comprising a first section structure and a second section structure,
the first stage structure having an opening for insertion of a glass pasteur pipette,
the second section structure is provided with a socket for inserting a pipette of a pipettor.
In a preferred embodiment, the inner surface of the opening of the connecting member has an arc-shaped configuration.
In a preferred embodiment, the helical blade is made of hard plastic and the annular sealing gasket is made of soft plastic.
Another aspect of the present invention is to provide a pipette having a connection part to which a glass pasteur pipette is connected, the pipette including a pipette control part, a pipette,
the connecting member includes:
an opening for inserting a glass pasteur pipette,
in the opening, a plurality of helical blades with elasticity are arrayed,
an annular sealing gasket, when the glass Pasteur pipette is screwed into the opening, the end part of the glass Pasteur pipette far away from the suction head is sealed with a pipette of the pipettor through the annular sealing gasket,
and the helical blade presses the outer surface of the glass pasteur pipette to tightly combine the connecting component with the outer surface of the glass pasteur pipette.
In a preferred embodiment, the connection part is of a conical structure and further comprises a socket for inserting a pipette of a pipette.
In a preferred embodiment, the connecting part is a hollow cylindrical structure, comprising a first section structure and a second section structure,
the first stage structure, having an opening for insertion of a glass pasteur pipette,
the second section structure is provided with a socket for inserting a pipette of a pipettor.
In a preferred embodiment, the inner surface of the opening of the connecting member has an arc-shaped configuration.
In a preferred embodiment, the helical blade is made of a hard plastic and the sealing gasket is made of a soft plastic.
In a preferred embodiment, the pipette further comprises a holder.
The connecting component and the liquid transfer device suitable for connecting the glass Pasteur pipette perfectly combine the liquid transfer device and the glass Pasteur pipette, and avoid using the plastic suction head of the existing liquid transfer device, which is toxic to oocytes or embryos.
The connecting component and the liquid transfer device suitable for connecting the glass Pasteur pipette perfectly combine the liquid transfer device and the glass Pasteur pipette, and the advantage that the quantitative control can be realized by the liquid transfer device and the advantage that the glass Pasteur pipette loads the oocyte or the embryo containing the cryoprotectant are utilized, so that the oocyte or the embryo can be controllably and quantitatively transferred onto the freezing carrying rod.
The invention provides a connecting part and a liquid transfer device suitable for connecting a glass Pasteur pipette, wherein a cryoprotectant loaded on a carrier rod has small volume and can be quantitatively (for example, fixed at 0.3 mu l); reduce the human error of the embryo freezing operation of the operator.
The connecting component suitable for connecting the glass pasteur pipette, the quantitative advantage of the pipette and the advantage that the glass pasteur pipette can smoothly transfer the oocyte or the embryo, which are provided by the invention, improve the defect that the glass pasteur pipette can not stably quantify, and effectively realize the purposes of quantitative standardization and innocuity of the cryoprotectant attached to the periphery of the oocyte or the embryo.
The connecting part and the liquid transfer device suitable for connecting the glass Pasteur suction pipe can effectively improve human errors existing in the freezing operation of different operators and different operations of the same operator, and realize the quality control of the freezing operation.
The connecting component and the liquid transfer device suitable for connecting the glass Pasteur suction pipe, provided by the invention, adopt a fixing frame structure, can assist a novice operation, realize the stability of operation and greatly reduce the training time and the training cost.
The connecting component and the liquid transfer device are suitable for connecting the pasteur pipette, the liquid transfer device is connected with the glass pasteur pipette, the defects of toxicity, non-smoothness and non-transparency of a plastic suction head are avoided, and the purposes of stably and effectively transferring and loading embryos are achieved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
Fig. 1 is a schematic view of a pipette in example 1 of the present invention.
FIG. 2 is a schematic view of a coupling member suitable for use in coupling glass Pasteur pipettes in example 1 of the present invention.
Fig. 3 isbase:Sub>A sectional viewbase:Sub>A-base:Sub>A in fig. 2.
Fig. 4 is a schematic view of a pipette in example 2 of the present invention.
FIG. 5 is a schematic view of a coupling member suitable for use in coupling glass Pasteur pipettes in example 2 of the present invention.
Fig. 6 is a view B-B in fig. 5.
FIG. 7 is a schematic view of a coupling member suitable for use in coupling glass Pasteur pipettes in example 3 of the present invention.
Fig. 8 is a cross-sectional view taken along line C-C of fig. 7.
Detailed Description
In order to make the aforementioned and other features and advantages of the invention more apparent, the invention is further described below with reference to the accompanying drawings. It is understood that the specific embodiments described herein are for purposes of illustration only and are not intended to be limiting, as those of ordinary skill in the art will recognize. In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Moreover, various embodiments or examples and features of various embodiments or examples described in this specification can be combined and combined by one skilled in the art without being mutually inconsistent.
In order to solve the defect that a glass pasteur pipette cannot stably quantify when oocytes or embryos are frozen and loaded on a freezing carrying rod in the prior art, the invention provides a connecting component suitable for connecting the glass pasteur pipette and a pipettor. Hereinafter, the present invention will be described in detail by way of specific examples.
Example 1.
Fig. 1 isbase:Sub>A schematic view ofbase:Sub>A pipette according to example 1 of the present invention, fig. 2 isbase:Sub>A schematic view ofbase:Sub>A connection member suitable for connectingbase:Sub>A glass pasteur pipette according to example 1 of the present invention, and fig. 3 isbase:Sub>A sectional view taken along linebase:Sub>A-base:Sub>A in fig. 2. Referring to fig. 1 to 3, the present invention provides a pipette having a connection member 3 adapted to connect a glass pasteur pipette, and a pipette body 1.
The pipette body 1 has a pipette control component, and the pipette control component in a specific embodiment includes a sample loading display interface 106 and a washing display interface 107, and sample loading or washing operation is set through adjusting buttons (not shown in the figure) on the sample loading interface 106 and the washing interface 107. Specifically, in the examples, the amount of the sample to be loaded may be adjusted to 0 to 2.5. Mu.l, and the amount of the wash may be adjusted to 2 to 20. Mu.l. As used herein, loading refers to aspiration of a fixed amount (e.g., 0.3. Mu.l) of the cryoprotectant-containing oocyte or embryo onto a cryorod. Washing refers to aspiration and expulsion of the cryoprotectant-containing oocyte or embryo (e.g., 20 μ l) to substantially displace the cryoprotectant.
The pipette control component further comprises a charging interface 105, a fuel port 108 and a rotation knob 109. The charging interface 105 is used for charging the pipettor, and the oil filling port 108 is used for adding oil to the pipettor. The rotary knob 109 is rotated to push the oil to move, and the oil pressure is used to control the front end of the attenuated Pasteur pipette to quantitatively suck and discharge the oocyte or embryo containing cryoprotectant, and the oocyte or embryo is loaded on the freezing carrying rod. The pipette of the present invention is a pipette capable of quantitatively controlling the suction and discharge of a trace amount of liquid in a pasteur pipette.
The connecting member 3 according to an embodiment of the present invention includes: an opening for insertion of a glass pasteur straw and an annular gasket 302. A plurality of helical blades 301 having elasticity are arrayed for insertion into the opening of the glass pasteur pipette.
And the annular sealing gasket 302, when the opening of the glass pasteur straw is inserted into the end 202, far away from the sucker, of the glass pasteur straw 2, the end 202, far away from the sucker, of the glass pasteur straw 2 is sealed with the pipette 102 of the pipettor through the annular sealing gasket 302.
In this embodiment, the connection component 3 is a conical structure, and further includes a socket for inserting a pipette of a pipette, and the pipette 102 is inserted into the socket and sealed by an annular sealing gasket 302.
The helical blade 301 presses the outer surface of the glass pasteur pipette 2 away from the tip 202 of the pipette tip, tightly joining the connecting member 3 to the outer surface of the glass pasteur pipette 2 away from the tip 202 of the pipette tip.
In some preferred embodiments, in order to achieve a tight coupling of the connecting member 3 with the glass pasteur pipette 2 and a higher tightness with the pipette 102, the helical blade 301 is made of hard plastic and the annular sealing gasket 302 is made of soft plastic. In other embodiments, the housing of the connecting part 3 may be made of metal.
According to an embodiment of the present invention, by rotating the knob 109 to allow the oil to enter the pipette 102, a pressure difference is created within the pipette, thereby allowing the tip 201 of the glass pasteur pipette 2 to aspirate or eject the oocyte or embryo.
According to the embodiment of the present invention, the pipette body 1 is further provided with a filter 101 for preventing oil from entering into the suction head 201 of the glass pasteur pipette 2.
According to the embodiment of the invention, the pipette body is further provided with the support 104, and the pipette is fixed through the support 104, so that the stability of operation by a novice is facilitated. In other embodiments, the support 104 can be rotated to achieve rotational adjustment of the support orientation in a 360 orientation.
The following describes a connection member suitable for connecting a glass pasteur pipette and a procedure for using the pipette in the embodiment of the present invention.
The specific suction amount of the sample loading or washing is set by the pipette control part including a sample loading display interface 106 and a washing display interface 107, and the display interface is provided with scales to display the specific sample loading amount and washing amount. In the examples, the amount of the sample to be applied may be adjusted to 0 to 2.5. Mu.l, and the amount of the wash may be adjusted to 2 to 20. Mu.l.
And (3) washing process:
the oocyte or embryo is washed (liquid displacement) before freezing the oocyte or embryo. The tip 201 of the glass Pasteur pipette 2 is brought into contact with the oocyte or embryo, the amount of washing is set to 2-20. Mu.l, for example, to 20. Mu.l, via the washing display interface 107, and the washing button is pressed. The knob 109 is rotated in the forward direction to cause the oil to flow back from the pipette 102, thereby creating a pressure difference at the tip 201 of the glass pasteur pipette 2, and aspirating the oocyte or embryo (20 μ l) containing the cryoprotectant into the tip 201 of the glass pasteur pipette 2. And rotating the knob 109 reversely to make the oil enter the pipette 102, forming a pressure difference in the glass Pasteur pipette 2, spitting the oocyte or embryo in the suction head 201 of the glass Pasteur pipette 2 into the No. 1 refrigerant, and standing for 5 minutes. The above process is repeated to suck the oocyte or embryo from the No. 1 freezing solution, and then the oocyte or embryo is insufflated into the No. 2 freezing preservative, and the oocyte or embryo is washed (liquid replacement) repeatedly for many times.
After multiple washes, the oocytes or embryos are frozen and loaded.
And (3) loading:
the sample loading display interface 106 sets a sample loading amount of 0 to 2.5. Mu.l, for example, a fixed amount of 0.3. Mu.l, and presses the sample loading button. The knob 109 is rotated in the forward direction to cause the oil to flow back from the pipette 102, thereby creating a pressure difference in the tip 201 of the glass pasteur pipette 2 and sucking the cryoprotectant (0.3. Mu.l) containing the oocyte or embryo into the tip 201 of the glass pasteur pipette 2. And then the knob 109 is rotated reversely to make the oil liquid enter the pipette 102, a pressure difference is formed in the glass pasteur pipette 2, the oocyte or embryo containing the cryoprotectant in the suction head 201 of the glass pasteur pipette 2 is discharged to the blade or the groove at the top of the freezing carrying rod or is discharged to a clean position of the flat dish 5, 0.3 microliter of cryoprotectant containing the oocyte or embryo is sucked into the straw by the siphoning action of the hollow straw 4 (similar to a disposable practical sterile distal blood collection tube, and the straw can be manually or mechanically drawn), and the cryoprotectant is rapidly put into liquid nitrogen at the temperature of 196 ℃ below zero for preservation. When in unfreezing, if the frozen rod is used, the frozen rod can be directly put into the unfreezing liquid 1 (1M sucrose solution); in the case of hollow straws, the top of the hollow straw 4 is blocked, the portion containing the cryoprotectant is inserted into the thawing solution 1 (1M sucrose solution), the top is released, and the oocyte or embryo is released into the thawing solution 1.
Example 2.
This embodiment is different from embodiment 1 in that the connection member of this embodiment is directly embedded in the pipette body. Fig. 4 is a schematic view of a pipette according to example 2 of the present invention, fig. 5 is a schematic view of a connection member suitable for connecting a glass pasteur pipette according to example 2 of the present invention, and fig. 6 is a view B-B of fig. 5.
According to an embodiment of the invention, the connection part 3a is directly embedded in the pipette body 1a, the connection part 3a comprising an opening for inserting a glass pasteur pipette and an annular sealing gasket 302a. A plurality of helical blades 301a having elasticity are arrayed in an opening for inserting a glass pasteur pipette. In this embodiment, the inner surface of the opening of the connecting member 3a for inserting the pasteur pipette is formed in an arc-shaped configuration.
The annular sealing gasket 302a seals with the pipette of the pipette by the annular sealing gasket 302a when the glass pasteur pipette is screwed into the opening for insertion of the pasteur pipette, away from the end of the tip. The screw blade 301a presses the outer surface of the glass pasteur pipette so that the coupling member is tightly coupled to the outer surface of the glass pasteur pipette.
In this embodiment, the filter 101 is provided in a glass pasteur pipette to prevent the oil in the pipette from entering the tip of the pasteur pipette.
Example 3.
The difference between this example and example 1 is that: the connecting member has a hollow cylindrical structure, and is shown in fig. 7, which is a schematic view of the connecting member suitable for connecting glass pasteur pipettes in example 3 of the present invention, and fig. 8, which is a cross-sectional view taken along line C-C in fig. 7.
In the present embodiment, the connecting member 3b is a hollow cylindrical structure including a first stage structure and a second stage structure. A first stage structure having an opening 305b for insertion of a glass pasteur pipette, and a second stage structure having a socket 304b for insertion of a pipette of a pipettor.
According to an embodiment of the invention, the connection part 3b comprises an opening for inserting a glass pasteur straw and an annular sealing gasket 302b. The opening for inserting the glass pasteur pipette is located in the first stage structure, internally arrayed with a plurality of helical blades 301b having elasticity.
An annular sealing gasket 302b seals with the pipette of the pipette with the annular sealing gasket 302b when the glass pasteur pipette is screwed into the opening for insertion of the pasteur pipette, away from the tip end 302b. The screw blade 301b presses the outer surface of the glass pasteur pipette so that the coupling member 3b is tightly coupled to the outer surface of the glass pasteur pipette.
In this embodiment, the connecting part 3b further has a chuck 303b, and the pipette is inserted into the socket 304b by being snapped into the pipette by the chuck 303 b. Specifically, the manner in which the collet 303b is engaged with the pipette may be selected by a person skilled in the art, and will not be described herein.
The connecting component and the liquid transfer device suitable for connecting the glass Pasteur pipette perfectly combine the liquid transfer device and the glass Pasteur pipette, and avoid using the plastic suction head of the existing liquid transfer device, which is toxic to oocytes or embryos.
The connecting component and the liquid transfer device are suitable for connecting the glass Pasteur pipette, the liquid transfer device and the glass Pasteur pipette are perfectly combined, the advantage that the quantitative control can be realized by the liquid transfer device and the advantage that the glass Pasteur pipette can blow and suck the oocyte or embryo containing the cryopreservative agent are utilized, and the quantitative transfer of the oocyte or embryo containing the cryopreservative agent onto the freezing carrying rod can be controlled.
The connecting part and the liquid transfer device suitable for connecting the glass Pasteur pipette have small volume of the cryoprotectant loaded on the carrier rod and can quantify (for example, the cryoprotectant is fixed to be 0.3 ul); reduce the human error of the embryo freezing operation of the operator.
The connecting component suitable for connecting the glass pasteur pipette, the liquid transfer device, the quantitative advantage of the liquid transfer device and the advantage that the glass pasteur pipette can smoothly transfer the oocyte or the embryo, which are provided by the invention, overcomes the defect that the glass pasteur pipette can not stably quantify, and effectively realizes the purposes of quantitative standardization and innocuity of the cryoprotectant attached to the periphery of the oocyte or the embryo.
The connecting part and the liquid transfer device suitable for connecting the pasteur pipette can effectively improve human errors existing between different operators and different operations of the same operator when the different operators perform freezing operations, and realize quality control of the freezing operation.
The connecting component and the liquid transfer device suitable for connecting the glass Pasteur suction pipe, which are provided by the invention, adopt a fixing frame structure, can assist a novice operation, realize the stability of the operation and greatly reduce the training time and the training cost.
The connecting component and the liquid transfer device are suitable for connecting the glass Pasteur pipette, the liquid transfer device is connected with the glass Pasteur pipette, the defects of toxicity, non-smoothness and non-transparency of a plastic suction head are avoided, and the purposes of stably and effectively transferring and loading embryos are achieved.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (5)
1. A pipettor with a connection part for connecting a glass Pasteur pipette, characterized in that the pipettor comprises a pipettor body, the pipettor body comprises a pipetting control part, a pipettor,
the pipettor control part comprises a sample loading display interface, a washing display interface, a charging interface, an oil filling interface and a rotary knob,
the knob is rotated to push the oil to move, and the oil pressure is adopted to control the quantitative suction or discharge of the oocyte or embryo containing the cryoprotectant at the front end of the glass pasteur pipette;
the connecting member includes:
an opening for inserting a glass pasteur pipette,
in the opening, a plurality of helical blades with elasticity are arrayed,
an annular sealing gasket, when the glass Pasteur pipette is screwed into the opening, the end part of the glass Pasteur pipette far away from the suction head is sealed with a pipette of the pipettor through the annular sealing gasket,
the spiral blade presses the outer surface of the glass pasteur pipette to make the connecting component and the outer surface of the glass pasteur pipette combine tightly;
the pipettor body is also provided with a filter disc to prevent oil from entering a suction head of the glass Pasteur suction pipe.
2. The pipette of claim 1, wherein the connection member is of a tapered configuration and further comprises a socket for inserting a pipette of a pipette.
3. The pipette of claim 1, wherein the connecting member is a hollow cylindrical structure including a first segment structure and a second segment structure,
the first stage structure having an opening for insertion of a glass pasteur pipette,
the second segment structure has a socket for inserting a pipette of a pipette.
4. The pipette of claim 1, wherein an inner surface of the opening of the connection member has an arc-shaped configuration.
5. The pipette according to any one of claims 1 to 4, wherein the screw blade is made of a hard plastic and the sealing gasket is made of a soft plastic.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2824871Y (en) * | 2005-01-18 | 2006-10-11 | 华中农业大学 | An embryo manipulation device |
| CN206659888U (en) * | 2016-12-26 | 2017-11-24 | 金华市人民医院 | Look for ovum, embryo transfer and wash smart device |
| CN208362346U (en) * | 2018-04-16 | 2019-01-11 | 湖南人文科技学院 | A kind of hand held embryo transfering device |
| CN208500961U (en) * | 2018-05-28 | 2019-02-15 | 湖南中医药大学 | A kind of novel pasteur pipet |
| CN210545208U (en) * | 2019-09-09 | 2020-05-19 | 马才启 | Liquid transfer device |
| CN210560436U (en) * | 2019-06-27 | 2020-05-19 | 湖南人文科技学院 | Pen type suction embryo tube |
| CN211546525U (en) * | 2019-11-18 | 2020-09-22 | 广州市妇女儿童医疗中心 | Suction head structure and embryo operating device |
| CN113226554A (en) * | 2018-12-17 | 2021-08-06 | 希尔根伯格有限公司 | Suction device for pipettor |
| CN213913854U (en) * | 2020-10-15 | 2021-08-10 | 华夏源细胞工程集团股份有限公司 | Novel pasteur straw |
| CN216796305U (en) * | 2022-03-08 | 2022-06-24 | 中国人民武装警察部队特色医学中心 | Connecting part suitable for connecting glass pasteur suction tube and liquid transfer device |
-
2022
- 2022-03-08 CN CN202210227008.6A patent/CN114431224B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2824871Y (en) * | 2005-01-18 | 2006-10-11 | 华中农业大学 | An embryo manipulation device |
| CN206659888U (en) * | 2016-12-26 | 2017-11-24 | 金华市人民医院 | Look for ovum, embryo transfer and wash smart device |
| CN208362346U (en) * | 2018-04-16 | 2019-01-11 | 湖南人文科技学院 | A kind of hand held embryo transfering device |
| CN208500961U (en) * | 2018-05-28 | 2019-02-15 | 湖南中医药大学 | A kind of novel pasteur pipet |
| CN113226554A (en) * | 2018-12-17 | 2021-08-06 | 希尔根伯格有限公司 | Suction device for pipettor |
| CN210560436U (en) * | 2019-06-27 | 2020-05-19 | 湖南人文科技学院 | Pen type suction embryo tube |
| CN210545208U (en) * | 2019-09-09 | 2020-05-19 | 马才启 | Liquid transfer device |
| CN211546525U (en) * | 2019-11-18 | 2020-09-22 | 广州市妇女儿童医疗中心 | Suction head structure and embryo operating device |
| CN213913854U (en) * | 2020-10-15 | 2021-08-10 | 华夏源细胞工程集团股份有限公司 | Novel pasteur straw |
| CN216796305U (en) * | 2022-03-08 | 2022-06-24 | 中国人民武装警察部队特色医学中心 | Connecting part suitable for connecting glass pasteur suction tube and liquid transfer device |
Non-Patent Citations (1)
| Title |
|---|
| 可调大容量移液器-可配巴斯德管适配器;生物器材网;《https://www.bio-equip.com/show1equip.asp?equipid=4002499》;20160922;第1-5页 * |
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| CN114431224A (en) | 2022-05-06 |
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