CN114426908B - Efficient biological enzyme cleaning agent and preparation method thereof - Google Patents
Efficient biological enzyme cleaning agent and preparation method thereof Download PDFInfo
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- CN114426908B CN114426908B CN202110579529.3A CN202110579529A CN114426908B CN 114426908 B CN114426908 B CN 114426908B CN 202110579529 A CN202110579529 A CN 202110579529A CN 114426908 B CN114426908 B CN 114426908B
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- biological enzyme
- cleaning agent
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- surfactant
- agent
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- 102000004190 Enzymes Human genes 0.000 title claims abstract description 166
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 166
- 239000012459 cleaning agent Substances 0.000 title claims abstract description 98
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 239000004094 surface-active agent Substances 0.000 claims abstract description 46
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 45
- 239000003381 stabilizer Substances 0.000 claims abstract description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 39
- 239000008367 deionised water Substances 0.000 claims abstract description 28
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 28
- 239000002994 raw material Substances 0.000 claims abstract description 14
- 229940088598 enzyme Drugs 0.000 claims description 174
- 239000002736 nonionic surfactant Substances 0.000 claims description 41
- 102000004882 Lipase Human genes 0.000 claims description 39
- 108090001060 Lipase Proteins 0.000 claims description 39
- 239000004367 Lipase Substances 0.000 claims description 39
- 235000019421 lipase Nutrition 0.000 claims description 39
- 108010059892 Cellulase Proteins 0.000 claims description 38
- 108091005804 Peptidases Proteins 0.000 claims description 38
- 239000004365 Protease Substances 0.000 claims description 38
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 38
- 229940106157 cellulase Drugs 0.000 claims description 38
- 150000003138 primary alcohols Chemical class 0.000 claims description 34
- 239000003945 anionic surfactant Substances 0.000 claims description 30
- 239000000203 mixture Substances 0.000 claims description 26
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical group C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 24
- 239000011734 sodium Substances 0.000 claims description 24
- 229910052708 sodium Inorganic materials 0.000 claims description 24
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 23
- 239000000600 sorbitol Substances 0.000 claims description 23
- -1 alkylbenzene sulfonate Chemical class 0.000 claims description 22
- 229920001450 Alpha-Cyclodextrin Polymers 0.000 claims description 21
- HFHDHCJBZVLPGP-RWMJIURBSA-N alpha-cyclodextrin Chemical group OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO HFHDHCJBZVLPGP-RWMJIURBSA-N 0.000 claims description 21
- 229940043377 alpha-cyclodextrin Drugs 0.000 claims description 21
- 150000004996 alkyl benzenes Chemical group 0.000 claims description 14
- 229940077388 benzenesulfonate Drugs 0.000 claims description 14
- 125000000129 anionic group Chemical group 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 13
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 claims description 12
- 239000000463 material Substances 0.000 abstract description 36
- 230000000694 effects Effects 0.000 abstract description 18
- 238000005202 decontamination Methods 0.000 abstract description 11
- 230000003588 decontaminative effect Effects 0.000 abstract description 10
- 230000007613 environmental effect Effects 0.000 abstract description 7
- 239000000126 substance Substances 0.000 abstract description 5
- 230000007794 irritation Effects 0.000 abstract description 4
- 231100000252 nontoxic Toxicity 0.000 abstract description 4
- 230000003000 nontoxic effect Effects 0.000 abstract description 4
- 230000007935 neutral effect Effects 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 description 42
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 33
- 229910052799 carbon Inorganic materials 0.000 description 33
- 239000000047 product Substances 0.000 description 23
- 238000000034 method Methods 0.000 description 20
- 238000012360 testing method Methods 0.000 description 18
- 229910000831 Steel Inorganic materials 0.000 description 13
- 239000010959 steel Substances 0.000 description 13
- 229920000858 Cyclodextrin Polymers 0.000 description 12
- 239000000758 substrate Substances 0.000 description 10
- 240000006439 Aspergillus oryzae Species 0.000 description 9
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- 241000589540 Pseudomonas fluorescens Species 0.000 description 9
- 241000499912 Trichoderma reesei Species 0.000 description 9
- FYGDTMLNYKFZSV-CSHPIKHBSA-N beta-cellotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-CSHPIKHBSA-N 0.000 description 9
- 238000011056 performance test Methods 0.000 description 8
- 239000001116 FEMA 4028 Substances 0.000 description 6
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 description 6
- 235000011175 beta-cyclodextrine Nutrition 0.000 description 6
- 229960004853 betadex Drugs 0.000 description 6
- 235000014113 dietary fatty acids Nutrition 0.000 description 6
- 239000000194 fatty acid Substances 0.000 description 6
- 229930195729 fatty acid Natural products 0.000 description 6
- GDSRMADSINPKSL-HSEONFRVSA-N gamma-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO GDSRMADSINPKSL-HSEONFRVSA-N 0.000 description 6
- 229940080345 gamma-cyclodextrin Drugs 0.000 description 6
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 5
- 229910021645 metal ion Inorganic materials 0.000 description 5
- 150000004665 fatty acids Chemical class 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid group Chemical group C(CC(O)(C(=O)O)CC(=O)O)(=O)O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 239000000693 micelle Substances 0.000 description 3
- 239000003208 petroleum Substances 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000007711 solidification Methods 0.000 description 3
- 230000008023 solidification Effects 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- XMGQYMWWDOXHJM-JTQLQIEISA-N (+)-α-limonene Chemical compound CC(=C)[C@@H]1CCC(C)=CC1 XMGQYMWWDOXHJM-JTQLQIEISA-N 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- 229920001732 Lignosulfonate Polymers 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 108010029182 Pectin lyase Proteins 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 150000008052 alkyl sulfonates Chemical class 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 2
- 150000001735 carboxylic acids Chemical class 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 239000008233 hard water Substances 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 231100000956 nontoxicity Toxicity 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 235000021317 phosphate Nutrition 0.000 description 2
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 2
- 150000003628 tricarboxylic acids Chemical class 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000004711 α-olefin Substances 0.000 description 2
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 125000001487 glyoxylate group Chemical group O=C([O-])C(=O)[*] 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 150000004965 peroxy acids Chemical class 0.000 description 1
- 230000005501 phase interface Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/83—Mixtures of non-ionic with anionic compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/0005—Other compounding ingredients characterised by their effect
- C11D3/0036—Soil deposition preventing compositions; Antiredeposition agents
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2003—Alcohols; Phenols
- C11D3/2065—Polyhydric alcohols
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/22—Carbohydrates or derivatives thereof
- C11D3/222—Natural or synthetic polysaccharides, e.g. cellulose, starch, gum, alginic acid or cyclodextrin
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38618—Protease or amylase in liquid compositions only
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/02—Anionic compounds
- C11D1/12—Sulfonic acids or sulfuric acid esters; Salts thereof
- C11D1/22—Sulfonic acids or sulfuric acid esters; Salts thereof derived from aromatic compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/72—Ethers of polyoxyalkylene glycols
-
- C11D2111/16—
-
- C11D2111/18—
Abstract
The efficient biological enzyme cleaning agent at least comprises the following preparation raw materials in parts by weight: 1-20 parts of biological enzyme, 3-25 parts of surfactant, 0.1-3 parts of biological enzyme stabilizer, 0.5-3 parts of anti-deposition agent and 30-100 parts of deionized water. The cleaning agent has the advantages that the biological enzyme component has good decontamination capability and can degrade stains, the cleaning agent is environment-friendly, pollution-free and self-degradable, the acting force between a base material and the stains is weakened by the cleaning agent through adding a certain amount of surfactant, the peelability and the solubility of the stains are improved, the stability and the activity of the biological enzyme are improved through adding a certain amount of biological enzyme stabilizer, the cleaning agent has long-acting decontamination capability, and the decontamination thoroughness of the cleaning agent is improved through adding a certain anti-deposition agent. The substances used in the technical scheme are green, non-toxic and degradable, have excellent environmental protection performance, basically have no influence on the activity of the biological enzyme, and the cleaning agent is neutral, has no irritation to the base material and the skin, and has better use effect.
Description
Technical Field
The invention belongs to the technical field of cleaning agents, and particularly relates to a high-efficiency biological enzyme cleaning agent and a preparation method thereof.
Background
The cleaning agent is an indispensable product for production and life of people, and particularly for the field of mechanical processing, petroleum is blood for generating power by mechanical tools, so that the cleaning agent is indispensable for the mechanical tools. Along with the long-term use of machine tool, the condition such as oil leak or oil stain pollution can take place, but current cleaner can not be fine to greasy dirt and the fine washing of oil stain is got rid of to current cleaner still contains the material that phosphor etc. are great to the environmental hazard, discharges into aquatic and forms non-degradable pollutant, can continuously pollute the water source.
The invention patent with the patent application number of 201410695665.9 discloses a bio-enzyme antibacterial emulsified oil stain preparation, which mainly comprises a surfactant, a chelating agent, an antirust preservative, triethanolamine, an environment-friendly organic solvent, a bactericide, D-limonene, bio-enzyme and deionized water. A cleaning agent which is degradable, has a good cleaning effect and is free of pollution is urgently needed.
Disclosure of Invention
In order to solve the technical problems, the invention provides a high-efficiency biological enzyme cleaning agent, which is prepared from the following raw materials in parts by weight: 1-20 parts of biological enzyme, 3-25 parts of surfactant, 0.1-3 parts of biological enzyme stabilizer, 0.5-3 parts of anti-deposition agent and 30-100 parts of deionized water.
Preferably, the biological enzyme is selected from one or more of lipase, protease, amylase, cellulase, pectin lyase and oxidoreductase.
Preferably, the biological enzyme is a mixture of lipase, protease and cellulase, and the mass ratio of the lipase, the protease and the cellulase is (10-15): (3-8): (1-5).
Preferably, the surfactant is a nonionic surfactant and/or an anionic surfactant.
Preferably, the surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is (10-15): 1.
preferably, the anionic surface is a sulfonate.
Preferably, the sulfonate is selected from one or more of alkyl benzene sulfonate, alpha-olefin sulfonate, alkyl sulfonate, alpha-sulfo monocarboxylic acid ester, fatty acid sulfoalkyl ester, succinate sulfonate, alkyl naphthalene sulfonate, petroleum sulfonate, lignin sulfonate and alkyl glyceryl ether sulfonate.
Preferably, the biological enzyme stabilizer contains at least one hydroxyl group.
Preferably, the anti-deposition agent is selected from one or more of carbonates, phosphates, amino acids, carboxylic acids and alcohols.
The second aspect of the invention provides a preparation method of the high-efficiency biological enzyme cleaning agent, which at least comprises the following steps: mixing biological enzyme, surfactant, biological enzyme stabilizer, anti-deposition agent and deionized water to obtain the biological enzyme-containing water-based detergent.
Has the beneficial effects that:
(1) Through using biological enzyme composition not only has better soil-release ability among this technical scheme, can get rid of the spot on various substrates fast, but also can degrade the spot, can not produce any burden to the environment, each component in the cleaner is green, environmental protection, nuisanceless moreover, and self degradable can not cause any pollution and pressure to the environment, belongs to green product, accords with the green's that the country advocated theory.
(2) The biological enzyme cleaning agent in the technical scheme is neutral as a whole, can be used for cleaning the surface of any base material, does not corrode the base material, enables the base material to be kept intact when stains are removed, has no irritation to the skin of an operator, and has a good mild decontamination effect on the stains on any base material.
(3) According to the technical scheme, the cleaning agent weakens the acting force between the base material and the stains by adding a certain amount of the surfactant, improves the strippability and the solubility of the stains, has a good removing effect on various stains, improves the stability and the activity of the biological enzyme by adding a certain amount of the biological enzyme stabilizer, enables the cleaning agent to have long-acting decontamination capability, and improves the decontamination thoroughness of the cleaning agent by adding a certain anti-deposition agent.
Detailed Description
For purposes of the following detailed description, it is to be understood that the invention may assume various alternative variations and step sequences, except where expressly specified to the contrary. Moreover, other than in any operating examples, or where otherwise indicated, all numbers expressing, for example, quantities of ingredients used in the specification and claims are to be understood as being modified in all instances by the term "about". Accordingly, unless indicated to the contrary, the numerical parameters set forth in the following specification and attached claims are approximations that may vary depending upon the desired properties to be obtained by the present invention. At the very least, and not as an attempt to limit the application of the doctrine of equivalents to the scope of the claims, each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.
Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as possible. Any numerical value, however, inherently contains certain errors necessarily resulting from the standard deviation found in their respective testing measurements.
When a range of values is disclosed herein, the range is considered to be continuous and includes both the minimum and maximum values of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values of the range is included. Further, when multiple range-describing features or characteristics are provided, the ranges may be combined. In other words, unless otherwise indicated, all ranges disclosed herein are to be understood to encompass any and all subranges subsumed therein. For example, a stated range from "1 to 10" should be considered to include any and all subranges between the minimum value of 1 and the maximum value of 10. Exemplary subranges of the range 1 to 10 include, but are not limited to, 1 to 6.1, 3.5 to 7.8, 5.5 to 10, and the like.
In order to solve the technical problems, the invention provides a high-efficiency biological enzyme cleaning agent, which is prepared from the following raw materials in parts by weight: 1-20 parts of biological enzyme, 3-25 parts of surfactant, 0.1-3 parts of biological enzyme stabilizer, 0.5-3 parts of anti-deposition agent and 30-100 parts of deionized water.
Biological enzymes
As a preferred technical solution, the biological enzyme is selected from one or more of lipase, protease, amylase, cellulase, pectin lyase and oxidoreductase.
As a preferable technical scheme, the biological enzyme is a mixture of lipase, protease and cellulase, and the mass ratio of the lipase, the protease and the cellulase is (10-15): (3-8): (1-5).
The biological enzyme is a nontoxic, environment-friendly and degradable biological catalyst, and the chemical essence of the biological enzyme is protein. The biological enzyme has specificityI.e., an enzyme can catalyze only one type of substance. The lipase can hydrolyze fat into glycerol and fatty acid, and the fatty acid is further subjected to B oxidation, one C is removed each time 2 The product generates acetyl COA (N-cyclohexyl octyl amine), enters a TCA (tricarboxylic acid) ring for complete oxidation or enters a glyoxylate ring for synthesizing saccharides. The lipase can effectively and rapidly decompose the grease, and has better cleaning property and environmental protection property when being used in a cleaning agent. The protease can decompose protein into polypeptide, so that protein stains are quickly decomposed, and the protease is green, safe and pollution-free. The cellulase can act on cellulose and cellulose derivatives thereof, so that insoluble cellulose is converted into soluble micromolecules such as glucose and the like, the fast cleaning of the cleaning agent to stains is facilitated to be improved, and the cellulase has better degradability and environmental protection performance. Because the biological enzyme has stronger specificity on catalyzing and cleaning different stains, in order to ensure that the cleaning agent has better removal capability on the stains formed in different places, the inventor discovers through screening and compounding that the lipase, the protease and the cellulase have better synergistic decontamination effect under a certain proportion. And the biological enzyme can be completely degraded without causing any harm to the environment. The technical scheme is mainly used for removing oil stains on various base materials, so that lipase is a biological enzyme main body, and ester bonds of triglyceride, diglyceride and monoglyceride are naturally catalyzed by the lipase and are hydrolyzed into fatty acid and glycerol, and the fatty acid and the glycerol also have activity on a wide range of base materials.
Surface active agent
As a preferred technical solution, the surfactant is a nonionic surfactant and/or an anionic surfactant.
As a preferable technical scheme, the surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is (10-15): 1.
as a preferred technical solution, the anionic area is a sulfonate.
As a preferable technical solution, the sulfonate is selected from one or more of alkyl benzene sulfonate, α -olefin sulfonate, alkyl sulfonate, α -sulfo monocarboxylic ester, fatty acid sulfoalkyl ester, succinate sulfonate, alkyl naphthalene sulfonate, petroleum sulfonate, lignin sulfonate and alkyl glyceryl ether sulfonate.
As a preferable technical scheme, the alkylbenzene sulfonate is sodium heavy alkylbenzene sulfonate.
As a preferred technical scheme, the nonionic surfactant is carbon 9-11 primary alcohol 8 ethoxylate.
The inventor finds that when the nonionic surfactant is selected, particularly the carbon 9-11 primary alcohol 8 ethoxylate is selected, the cleaning agent has a good cleaning effect on stains, and the physical properties of the carbon 9-11 primary alcohol 8 ethoxylate are mild, non-toxic, harmless, green and environment-friendly, so that the activity of the biological enzyme is not influenced. The molecular structure of the carbon 9-11 primary alcohol 8 ethoxylate has amphipathy, one end of the carbon 9-11 primary alcohol 8 ethoxylate is a hydrophilic group, the other end of the carbon 9-11 primary alcohol 8 ethoxylate is an oleophilic group, and the surface tension of the cleaning agent and oil stains can be well reduced by the carbon 9-11 primary alcohol 8 ethoxylate. The alkyl chain segment in the carbon 9-11 primary alcohol 8 ethoxylate is used as lipophilic group and can be dissolved in oil but can not be dissolved in water, and the polar group hydroxyl connected with the lipophilic chain segment is used as hydrophilic group and can be dissolved in water but can not be dissolved in oil. Due to the two-phase characteristics of the carbon 9-11 primary alcohol 8 ethoxylate, the carbon 9-11 primary alcohol 8 ethoxylate generates oriented arrangement on an oil-water phase interface to form an adsorption film, so that the surface tension or the interfacial tension is reduced. In aqueous solution, the molecules of the carbon 9-11 primary alcohol 8 ethoxylate are aggregated and regularly and directionally arranged to form micelles, wherein the hydrophilic groups are arranged outside and the lipophilic groups are arranged inside. When the cleaning agent is used for washing, hydrophilic groups of micelles formed by the carbon 9-11 primary alcohol 8 ethoxylate adsorb and permeate into the surface of a cleaned substrate, so that the attraction between oil stains and the cleaned substrate is reduced and eliminated, and the interaction force between oil stain molecules is also destroyed. Because the interaction force between the oleophilic group and the oil stain is larger, the oleophilic group can attract and dissolve the oil stain in the micelle, thereby achieving the effect of stripping and removing the oil stain on the surface of the base material. The inventor surprisingly finds that when a certain amount of anionic surfactant is added, particularly a certain amount of sodium heavy alkylbenzene sulfonate is added, the dissolubility of oil stains can be improved well, the decontamination efficiency of the cleaning agent is improved, and the phenomenon of rusting after the metal base material is cleaned can be prevented. The carbon 9-11 primary alcohol 8 ethoxylate and the heavy alkyl benzene sulfonate are green, non-toxic and degradable substances, and are added into the cleaning agent, so that the cleaning agent has high environmental protection performance. However, the inventors found that the addition of sodium heavy alkylbenzene sulfonate has a certain effect on the activity of the biological enzyme.
Biological enzyme stabilizer
As a preferred technical scheme, the biological enzyme stabilizer at least contains one hydroxyl group.
As a preferred technical scheme, the biological enzyme stabilizer is alpha-cyclodextrin.
The inventor finds that the activity of the biological enzyme can be effectively maintained by adding a certain amount of alpha-cyclodextrin into the cleaning agent, so that the biological enzyme has long-term decontamination capability and stability. Alpha-cyclodextrin is a hollow cylinder three-dimensional ring structure with a slightly conical shape, the upper end and the lower end of the hollow cylinder three-dimensional ring structure are provided with hydroxyl groups, the alpha-cyclodextrin is hydrophilic, when the alpha-cyclodextrin is added into a cleaning agent, the hydroxyl groups and amino acid chain segments in biological enzyme can form hydrogen bonds to be adsorbed on the biological enzyme, so that three-dimensional space barrier is formed, the biological enzyme is protected, the influence of an anionic surfactant on the biological enzyme is shielded, the activity of the biological enzyme is prolonged, the long-term stable decontamination capability of the cleaning agent is improved, and the irritation of the cleaning agent to a base material can be reduced. However, the inventors found that the cleaning efficiency of the cleaning agent is reduced when beta-cyclodextrin and gamma-cyclodextrin are selected, and the inventors believe that the possible reason is that the molecular weight of the beta-cyclodextrin and the gamma-cyclodextrin is large, and when the beta-cyclodextrin and the gamma-cyclodextrin are adsorbed on the surface of the biological enzyme, the beta-cyclodextrin and the gamma-cyclodextrin bring large resistance to the catalytic activity of the biological enzyme, and the catalytic effect of the biological enzyme is influenced. And the alpha-cyclodextrin has better water solubility, environmental protection, no toxicity and high degradability. The inventors have found that during cleaning with a cleaning agent, a small amount of deposits may form on the surface of the substrate being cleaned. The inventor believes that the probable reason is that the substrate is washed by partial geological water in the decontamination process, metal ions such as calcium, magnesium, zinc and the like are contained in addresses, and insoluble impurities are generated by the metal ions in the geological water and substances in the cleaning agent and are deposited on the surface of the substrate.
Anti-deposition agent
As a preferable technical scheme, the anti-deposition agent is selected from one or more of carbonates, phosphates, amino acids, carboxylic acids and alcohols.
As a preferred embodiment, the alcohol is sorbitol.
Although the acidic or alkaline anti-deposition agent can well complex metal ions in hard water, the peracid or over-alkaline environment of the cleaning agent can affect the activity of the biological enzyme. The inventor finds that the sorbitol is used as the anti-deposition agent, not only has better anti-deposition effect, but also can improve the high-temperature resistance stability of the cleaning agent. The sorbitol has good water solubility, non-toxicity and biodegradability, can form a stable chelate with metal ions, and prevents the metal ions in hard water from reacting with the remainder to generate deposits which are deposited on the surface of a cleaned substrate. And as sorbitol is a polyhydroxy compound, the cleaning agent can be adsorbed on the surface of the biological enzyme when not used, so that the spatial structure of the biological enzyme is stabilized, aggregation of the biological enzyme caused by change of external conditions is reduced, the stability and heat resistance of the biological enzyme are improved, and the biological enzyme in the cleaning agent cannot be coagulated into blocks and precipitated in the cleaning agent at a slightly high temperature.
Preparation method of biological enzyme cleaning agent
The second aspect of the invention provides a preparation method of the high-efficiency biological enzyme cleaning agent, which at least comprises the following steps: mixing biological enzyme, surfactant, biological enzyme stabilizer, anti-deposition agent and deionized water to obtain the product.
The third aspect of the invention provides a use method of the biological enzyme cleaning agent, which comprises the following steps: the biological enzyme cleaning agent is sprayed on the surface of a base material to be cleaned within the range of 20-60 ℃, and the surface of the base material is cleaned by using a cleaning tool after standing for 3-5 min.
The cleaning agent prepared by the invention is neutral, has no corrosion to the cleaned base material, and has no irritation to the skin of an operator.
In addition, the starting materials used are all commercially available, unless otherwise specified.
Example 1
In order to solve the above technical problems, a first aspect of this embodiment provides a high-efficiency biological enzyme cleaning agent, which comprises the following raw materials in parts by weight: 14 parts of biological enzyme, 8.8 parts of surfactant, 2 parts of biological enzyme stabilizer, 0.5 part of anti-deposition agent and 74.7 parts of deionized water. The biological enzyme is a mixture of lipase, protease and cellulase, and the mass ratio of the lipase, the protease and the cellulase is 10:3:1. the surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is 10:1, the anionic area is sodium heavy alkyl benzene sulfonate, the nonionic surfactant is a carbon 9-11 primary alcohol 8 ethoxylate, the biological enzyme stabilizer is alpha-cyclodextrin, and the anti-deposition agent is sorbitol. The lipase is derived from pseudomonas fluorescens and purchased from alatin, CAS No.: 9001-62-1. The protease is derived from aspergillus oryzae and purchased from alatin, CAS No.: 9014-01-1. The cellulase is derived from trichoderma reesei and purchased from alatin, CAS No.: 9012-54-8. The carbon 9-11 primary alcohol 8 ethoxylate is available from north Hu Wan chemical Co., ltd, CAS number: 68439-45-2. The sodium heavy alkylbenzene sulfonate is purchased from Nantong Runfeng petrochemical company Limited, and has a product number of: 27177-79-3. The alpha-cyclodextrin was purchased from Shandong Riche Biotech limited, brand: and (4) rich standing grain. The sorbitol was purchased from north Heibei Runshough Biotech limited, CAS number: 50-60-4.
The second aspect of this embodiment provides a preparation method of the high-efficiency bio-enzyme cleaning agent, which comprises: mixing biological enzyme, surfactant, biological enzyme stabilizer, anti-deposition agent and deionized water to obtain the product.
The third aspect of the embodiment provides a use method of the biological enzyme cleaning agent, which comprises the following steps: the biological enzyme cleaning agent is sprayed on the surface of a base material to be cleaned within the range of 20-60 ℃, and the surface of the base material is cleaned by using a cleaning tool after standing for 3-5 min.
Example 2
In order to solve the above technical problems, a first aspect of this embodiment provides a high-efficiency biological enzyme cleaning agent, which comprises the following raw materials in parts by weight: 14 parts of biological enzyme, 8.8 parts of surfactant, 2 parts of biological enzyme stabilizer, 0.5 part of anti-deposition agent and 74.7 parts of deionized water. The biological enzyme is a mixture of lipase, protease and cellulase, and the mass ratio of the lipase, the protease and the cellulase is 15:8:5. the surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is 10:1, the anionic area is sodium heavy alkyl benzene sulfonate, the nonionic surfactant is a carbon 9-11 primary alcohol 8 ethoxylate, the biological enzyme stabilizer is alpha-cyclodextrin, and the anti-deposition agent is sorbitol. The lipase is derived from pseudomonas fluorescens and purchased from alatin, CAS No.: 9001-62-1. The protease is derived from aspergillus oryzae and purchased from alatin, CAS No.: 9014-01-1. The cellulase is derived from trichoderma reesei and purchased from alatin, CAS No.: 9012-54-8. The carbon 9-11 primary alcohol 8 ethoxylate is available from north Hu Wan chemical Co., ltd, CAS number: 68439-45-2. The sodium heavy alkylbenzene sulfonate is purchased from Nantong Runfeng petrochemical company Limited, and has a product number of: 27177-79-3. The alpha-cyclodextrin was purchased from Shandong Riche Biotech limited, brand: and (4) rich standing grain. The sorbitol is purchased from north Heibei Runshou Biotech, inc., CAS number: 50-60-4.
The second aspect of this embodiment provides a preparation method of the high-efficiency bio-enzyme cleaning agent, which comprises: mixing biological enzyme, surfactant, biological enzyme stabilizer, anti-deposition agent and deionized water to obtain the product.
The third aspect of the embodiment provides a use method of the biological enzyme cleaning agent, which comprises the following steps: the biological enzyme cleaning agent is sprayed on the surface of a base material to be cleaned within the range of 20-60 ℃, and the surface of the base material is cleaned by using a cleaning tool after standing for 3-5 min.
Example 3
In order to solve the above technical problems, a first aspect of this embodiment provides a high-efficiency biological enzyme cleaning agent, which comprises the following raw materials in parts by weight: 14 parts of biological enzyme, 8.8 parts of surfactant, 2 parts of biological enzyme stabilizer, 0.5 part of anti-deposition agent and 74.7 parts of deionized water. The biological enzyme is a mixture of protease and cellulase, and the mass ratio of the protease to the cellulase is 3:1. the surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is 10:1, the anionic area is sodium heavy alkyl benzene sulfonate, the nonionic surfactant is a carbon 9-11 primary alcohol 8 ethoxylate, the biological enzyme stabilizer is alpha-cyclodextrin, and the anti-deposition agent is sorbitol. The protease is derived from aspergillus oryzae and purchased from alatin, CAS No.: 9014-01-1. The cellulase is derived from trichoderma reesei and purchased from alatin, CAS No.: 9012-54-8. The carbon 9-11 primary alcohol 8 ethoxylate is available from north Hu Wan chemical Co., ltd, CAS number: 68439-45-2. The sodium heavy alkylbenzene sulfonate is purchased from Nantong Runfeng petrochemical company Limited, and has a product number of: 27177-79-3. The alpha-cyclodextrin was purchased from Shandong Riche Biotech limited, brand: and (4) grain enrichment. The sorbitol was purchased from north Heibei Runshough Biotech limited, CAS number: 50-60-4.
The second aspect of this embodiment provides a preparation method of the high-efficiency bio-enzyme cleaning agent, which comprises: mixing biological enzyme, surfactant, biological enzyme stabilizer, anti-deposition agent and deionized water to obtain the product.
The third aspect of the embodiment provides a use method of the biological enzyme cleaning agent, which comprises the following steps: the biological enzyme cleaning agent is sprayed on the surface of a base material to be cleaned within the range of 20-60 ℃, and a cleaning tool is used for cleaning the surface of the base material after the biological enzyme cleaning agent is stood for 3-5 min.
Example 4
In order to solve the above technical problems, a first aspect of this embodiment provides a high-efficiency biological enzyme cleaning agent, which comprises the following raw materials in parts by weight: 14 parts of biological enzyme, 8.8 parts of surfactant, 2 parts of biological enzyme stabilizer, 0.5 part of anti-deposition agent and 74.7 parts of deionized water. The biological enzyme is lipase. The surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is 10:1, the anionic area is sodium heavy alkyl benzene sulfonate, the nonionic surfactant is carbon 9-11 primary alcohol 8 ethoxylate, the biological enzyme stabilizer is alpha-cyclodextrin, and the anti-deposition agent is sorbitol. The lipase is derived from pseudomonas fluorescens and purchased from alatin, CAS No.: 9001-62-1. The carbon 9-11 primary alcohol 8 ethoxylate is available from north Hu Wan chemical Co., ltd, CAS number: 68439-45-2. The sodium heavy alkylbenzene sulfonate is purchased from Nantong Runfeng petrochemical company Limited, and has a product number of: 27177-79-3. The alpha-cyclodextrin was purchased from Shandong Riche Biotech limited, brand: and (4) rich standing grain. The sorbitol was purchased from north Heibei Runshough Biotech limited, CAS number: 50-60-4.
The second aspect of this embodiment provides a preparation method of the high-efficiency bio-enzyme cleaning agent, which comprises: mixing biological enzyme, surfactant, biological enzyme stabilizer, anti-deposition agent and deionized water to obtain the product.
The third aspect of the embodiment provides a use method of the biological enzyme cleaning agent, which comprises the following steps: the biological enzyme cleaning agent is sprayed on the surface of a base material to be cleaned within the range of 20-60 ℃, and the surface of the base material is cleaned by using a cleaning tool after standing for 3-5 min.
Example 5
In order to solve the above technical problems, a first aspect of this embodiment provides a high-efficiency biological enzyme cleaning agent, which comprises the following raw materials in parts by weight: 14 parts of biological enzyme, 8.8 parts of surfactant, 2 parts of biological enzyme stabilizer, 0.5 part of anti-deposition agent and 74.7 parts of deionized water. The biological enzyme is a mixture of lipase, protease and cellulase, and the mass ratio of the lipase, the protease and the cellulase is 10:3:1. the surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is 7:1, the anionic area is sodium heavy alkyl benzene sulfonate, the nonionic surfactant is a carbon 9-11 primary alcohol 8 ethoxylate, the biological enzyme stabilizer is alpha-cyclodextrin, and the anti-deposition agent is sorbitol. The lipase is derived from pseudomonas fluorescens and purchased from alatin, CAS No.: 9001-62-1. The protease is derived from aspergillus oryzae and purchased from alatin, CAS No.: 9014-01-1. The cellulase is derived from trichoderma reesei and purchased from alatin, CAS No.: 9012-54-8. The carbon 9-11 primary alcohol 8 ethoxylate is available from north Hu Wan chemical Co., ltd, CAS number: 68439-45-2. The sodium heavy alkylbenzene sulfonate is purchased from Nantong Runfeng petrochemical company Limited, and has a product number of: 27177-79-3. The alpha-cyclodextrin was purchased from Shandong Riche Biotech limited, brand: and (4) rich standing grain. The sorbitol was purchased from north Heibei Runshough Biotech limited, CAS number: 50-60-4.
The second aspect of this embodiment provides a preparation method of the high-efficiency bio-enzyme cleaning agent, which comprises: mixing biological enzyme, surfactant, biological enzyme stabilizer, anti-deposition agent and deionized water to obtain the product.
The third aspect of the present embodiment provides a method for using the bio-enzyme cleaning agent: the biological enzyme cleaning agent is sprayed on the surface of a base material to be cleaned within the range of 20-60 ℃, and the surface of the base material is cleaned by using a cleaning tool after standing for 3-5 min.
Example 6
In order to solve the technical problems, a first aspect of the present embodiment provides a high-efficiency biological enzyme cleaning agent, which comprises the following raw materials by weight: 14 parts of biological enzyme, 8.8 parts of surfactant, 2 parts of biological enzyme stabilizer, 0.5 part of anti-deposition agent and 74.7 parts of deionized water. The biological enzyme is a mixture of lipase, protease and cellulase, and the mass ratio of the lipase, the protease and the cellulase is 10:3:1. the surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is 8.36:0.44, the anionic area is sodium heavy alkyl benzene sulfonate, the nonionic surfactant is carbon 9-11 primary alcohol 8 ethoxylate, the biological enzyme stabilizer is alpha-cyclodextrin, and the anti-deposition agent is sorbitol. The lipase is derived from pseudomonas fluorescens and purchased from alatin, CAS No.: 9001-62-1. The protease is derived from aspergillus oryzae and purchased from alatin, CAS No.: 9014-01-1. The cellulase is derived from trichoderma reesei and purchased from alatin, CAS No.: 9012-54-8. The carbon 9-11 primary alcohol 8 ethoxylate is available from north Hu Wan chemical Co., ltd, CAS number: 68439-45-2. The sodium heavy alkylbenzene sulfonate is purchased from Nantong Runfeng petrochemical company Limited, and has a product number of: 27177-79-3. The alpha-cyclodextrin was purchased from Shandong Fuhe Biotech, inc., brand: and (4) rich standing grain. The sorbitol was purchased from north Heibei Runshough Biotech limited, CAS number: 50-60-4.
The second aspect of this embodiment provides a preparation method of the high-efficiency bio-enzyme cleaning agent, which comprises: mixing biological enzyme, surfactant, biological enzyme stabilizer, anti-deposition agent and deionized water to obtain the product.
The third aspect of the embodiment provides a use method of the biological enzyme cleaning agent, which comprises the following steps: the biological enzyme cleaning agent is sprayed on the surface of a base material to be cleaned within the range of 20-60 ℃, and a cleaning tool is used for cleaning the surface of the base material after the biological enzyme cleaning agent is stood for 3-5 min.
Example 7
In order to solve the above technical problems, a first aspect of this embodiment provides a high-efficiency biological enzyme cleaning agent, which comprises the following raw materials in parts by weight: 14 parts of biological enzyme, 8.8 parts of surfactant, 0.5 part of anti-deposition agent and 74.7 parts of deionized water. The biological enzyme is a mixture of lipase, protease and cellulase, and the mass ratio of the lipase, the protease and the cellulase is 10:3:1. the surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is 10:1, the anionic area is sodium heavy alkyl benzene sulfonate, the nonionic surfactant is carbon 9-11 primary alcohol 8 ethoxylate, and the anti-deposition agent is sorbitol. The lipase is derived from pseudomonas fluorescens and purchased from alatin, CAS No.: 9001-62-1. The protease is derived from aspergillus oryzae and is available from alatin, CAS No.: 9014-01-1. The cellulase is derived from trichoderma reesei and purchased from alatin, CAS No.: 9012-54-8. The carbon 9-11 primary alcohol 8 ethoxylate is available from north Hu Wan chemical Co., ltd, CAS number: 68439-45-2. The sodium heavy alkylbenzene sulfonate is purchased from Nantong Runfeng petrochemical company Limited, and has a product number of: 27177-79-3. The sorbitol was purchased from north Heibei Runshough Biotech limited, CAS number: 50-60-4.
The second aspect of this embodiment provides a preparation method of the high-efficiency bio-enzyme cleaning agent, which comprises: mixing the biological enzyme, the surfactant, the anti-deposition agent and the deionized water to obtain the biological enzyme-containing water.
The third aspect of the present embodiment provides a method for using the bio-enzyme cleaning agent: the biological enzyme cleaning agent is sprayed on the surface of a base material to be cleaned within the range of 20-60 ℃, and the surface of the base material is cleaned by using a cleaning tool after standing for 3-5 min.
Example 8
In order to solve the above technical problems, a first aspect of this embodiment provides a high-efficiency biological enzyme cleaning agent, which comprises the following raw materials in parts by weight: 14 parts of biological enzyme, 8.8 parts of surfactant, 2 parts of biological enzyme stabilizer, 0.5 part of anti-deposition agent and 74.7 parts of deionized water. The biological enzyme is a mixture of lipase, protease and cellulase, and the mass ratio of the lipase, the protease and the cellulase is 10:3:1. the surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is 10:1, the anionic area is sodium heavy alkyl benzene sulfonate, the nonionic surfactant is a carbon 9-11 primary alcohol 8 ethoxylate, the biological enzyme stabilizer is beta-cyclodextrin, and the anti-deposition agent is sorbitol. The lipase is derived from pseudomonas fluorescens and purchased from alatin, CAS No.: 9001-62-1. The protease is derived from aspergillus oryzae and purchased from alatin, CAS No.: 9014-01-1. The cellulase is derived from trichoderma reesei and purchased from alatin, CAS No.: 9012-54-8. The carbon 9-11 primary alcohol 8 ethoxylate is available from north Hu Wan chemical Co., ltd, CAS number: 68439-45-2. The sodium heavy alkylbenzene sulfonate is purchased from Nantong Runfeng petrochemical company Limited, and has a product number of: 27177-79-3. The beta-cyclodextrin was purchased from Zhengzhou Kang Yuanhua products of Industrial, inc. The sorbitol was purchased from north Heibei Runshough Biotech limited, CAS number: 50-60-4.
The second aspect of this embodiment provides a preparation method of the high-efficiency bio-enzyme cleaning agent, which comprises: mixing biological enzyme, surfactant, biological enzyme stabilizer, anti-deposition agent and deionized water to obtain the product.
The third aspect of the present embodiment provides a method for using the bio-enzyme cleaning agent: the biological enzyme cleaning agent is sprayed on the surface of a base material to be cleaned within the range of 20-60 ℃, and the surface of the base material is cleaned by using a cleaning tool after standing for 3-5 min.
Example 9
In order to solve the above technical problems, a first aspect of this embodiment provides a high-efficiency biological enzyme cleaning agent, which comprises the following raw materials in parts by weight: 14 parts of biological enzyme, 8.8 parts of surfactant, 2 parts of biological enzyme stabilizer, 0.5 part of anti-deposition agent and 74.7 parts of deionized water. The biological enzyme is a mixture of lipase, protease and cellulase, and the mass ratio of the lipase, the protease and the cellulase is 10:3:1. the surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is 10:1, the anionic area is sodium heavy alkyl benzene sulfonate, the nonionic surfactant is a carbon 9-11 primary alcohol 8 ethoxylate, the biological enzyme stabilizer is gamma-cyclodextrin, and the anti-deposition agent is sorbitol. The lipase is derived from pseudomonas fluorescens and purchased from alatin, CAS No.: 9001-62-1. The protease is derived from aspergillus oryzae and is available from alatin, CAS No.: 9014-01-1. The cellulase is derived from trichoderma reesei and purchased from alatin, CAS No.: 9012-54-8. The carbon 9-11 primary alcohol 8 ethoxylate is available from north Hu Wan chemical Co., ltd, CAS number: 68439-45-2. The sodium heavy alkylbenzene sulfonate is purchased from Nantong Runfeng petrochemical company Limited, and has a product number of: 27177-79-3. The gamma-cyclodextrin was purchased from Shandong Riche Biotech limited, brand: and (4) rich standing grain. The sorbitol was purchased from north Heibei Runshough Biotech limited, CAS number: 50-60-4.
The second aspect of this embodiment provides a preparation method of the high-efficiency biological enzyme cleaning agent, which comprises: mixing biological enzyme, surfactant, biological enzyme stabilizer, anti-deposition agent and deionized water to obtain the product.
The third aspect of the embodiment provides a use method of the biological enzyme cleaning agent, which comprises the following steps: the biological enzyme cleaning agent is sprayed on the surface of a base material to be cleaned within the range of 20-60 ℃, and the surface of the base material is cleaned by using a cleaning tool after standing for 3-5 min.
Example 10
In order to solve the above technical problems, a first aspect of this embodiment provides a high-efficiency biological enzyme cleaning agent, which comprises the following raw materials in parts by weight: 14 parts of biological enzyme, 8.8 parts of surfactant, 2 parts of biological enzyme stabilizer, 0.5 part of anti-deposition agent and 74.7 parts of deionized water. The biological enzyme is a mixture of lipase, protease and cellulase, and the mass ratio of the lipase, the protease and the cellulase is 10:3:1. the surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is 10:1, the anionic area is sodium heavy alkyl benzene sulfonate, the nonionic surfactant is carbon 9-11 primary alcohol 8 ethoxylate, the biological enzyme stabilizer is alpha-cyclodextrin, and the anti-deposition agent is citric acid. Lipases were derived from pseudomonas fluorescens, purchased from alatin, CAS No.: 9001-62-1. The protease is derived from aspergillus oryzae and purchased from alatin, CAS No.: 9014-01-1. The cellulase is derived from trichoderma reesei and purchased from alatin, CAS No.: 9012-54-8. The carbon 9-11 primary alcohol 8 ethoxylate is available from north Hu Wan chemical Co., ltd, CAS number: 68439-45-2. The sodium heavy alkylbenzene sulfonate is purchased from Nantong Runfeng petrochemical company Limited, and has a product number of: 27177-79-3. The alpha-cyclodextrin was purchased from Shandong Riche Biotech limited, brand: and (4) rich standing grain.
The second aspect of this embodiment provides a preparation method of the high-efficiency biological enzyme cleaning agent, which comprises: mixing biological enzyme, surfactant, biological enzyme stabilizer, anti-deposition agent and deionized water to obtain the biological enzyme-containing water-based detergent.
The third aspect of the embodiment provides a use method of the biological enzyme cleaning agent, which comprises the following steps: the biological enzyme cleaning agent is sprayed on the surface of a base material to be cleaned within the range of 20-60 ℃, and the surface of the base material is cleaned by using a cleaning tool after standing for 3-5 min.
Performance test
Performance test one
Stability: the bio-enzyme cleaning agent prepared in example 1 to example 10 was placed in an environment of 65 ℃ for 24 hours, and the presence or absence of precipitation or solidification of the bio-enzyme cleaning agent was observed, and the presence or absence of precipitation or solidification was recorded as unstable, and the absence or absence of precipitation or solidification was recorded as stable, and the test results are shown in table 1.
Performance test 2
Cleaning efficiency at 20 ℃: (1) Preparing artificial oil stain according to a test standard JBT 4323.2-1999;
(2) 100 steel sheets with the same size are prepared and divided into 10 groups, and the cleaning efficiency test is respectively carried out by using the biological enzyme cleaning agent prepared in the embodiment 1-the embodiment 10, and the specific operation steps are as follows: placing each group of steel sheets in an oven at 20 ℃ for 8h, taking out, placing at room temperature for 3h, weighing the respective weight and recording as m1, then placing the steel sheets in artificial oil stain for soaking for 5min, lifting the steel sheets to be tested out by using a lifter, weighing the mass of the oil-soaked steel sheets when oil does not drip, weighing the respective weight and recording as m2 for later use; the bio-enzyme cleaning agents prepared in examples 1 to 10 were respectively prepared in the same amount in a constant temperature swing bath, the temperature was heated to 20 ℃, then the oil-soaked and weighed steel sheets were placed in a stable swing bath with a swing frequency of 30 times/min for 3min, then the steel sheets were placed in an oven at 20 ℃ for 8h, after being taken out, the steel sheets were placed at room temperature for 3h, the respective weights were weighed and recorded as m3, and the cleaning efficiency = [ (m 2-m 3)/(m 2-m 1) ] 100%, wherein the average cleaning efficiency of each group of steel sheets was 98% or more and recorded as excellent cleaning efficiency, wherein the average cleaning efficiency of each group of steel sheets was 90 to 98% and recorded as excellent cleaning efficiency, wherein the average cleaning efficiency of each group of steel sheets was less than 90% and recorded as poor cleaning efficiency, and the test results are shown in table 1.
Performance test three
Cleaning efficiency at 60 ℃: the method for testing the cleaning efficiency at 60 ℃ is the same as the method for testing the cleaning efficiency at 20 ℃, the temperature of the biological enzyme cleaning agent is only required to be heated to 60 ℃ for use, and the test results are shown in table 1.
Performance test four
Cleaning efficiency at 10 ℃: the method for testing the cleaning efficiency at 10 ℃ is the same as the method for testing the cleaning efficiency at 20 ℃, the temperature of the biological enzyme cleaning agent is only required to be regulated to 10 ℃ for use, and the test results are shown in table 1.
Performance test five
Cleaning efficiency at 70 ℃: the method for cleaning efficiency at 70 ℃ is the same as the method for testing the cleaning efficiency at 20 ℃, the temperature of the biological enzyme cleaning agent is only required to be heated to 70 ℃ for use, and the test results are shown in table 1.
Performance test six
Glass substrate 20 ℃ cleaning efficiency: the method for testing the cleaning efficiency of the glass substrate at 20 ℃ is the same as the method for testing the cleaning efficiency of the steel sheet substrate at 20 ℃, all steel sheets are only required to be replaced by glass, and the test results are shown in table 1.
Performance test seven
And (3) testing the degradation rate: reference ISO9408:2005, test method for evaluating the maximum aerobic biodegradability of organic compounds in aqueous media by determination of oxygen demand in a closed air permeability tester, wherein the degradation rate > 75% is reported as degradability pass, the degradation rate < 75% is reported as degradability fail, and the test results are given in table 1.
TABLE 1
From the above data, it is understood that the present invention is only a preferred embodiment of the present invention, and not limited to other forms, and any person skilled in the art may change or modify the technical content disclosed in the above to equivalent embodiments with equivalent changes, but all simple modifications, equivalent changes and modifications made to the above embodiments according to the technical essence of the present invention do not depart from the technical content of the present invention, and still belong to the protection scope of the present invention.
Claims (2)
1. The efficient biological enzyme cleaning agent is characterized by at least comprising the following raw materials in parts by weight: 1-20 parts of biological enzyme, 3-25 parts of surfactant, 0.1-3 parts of biological enzyme stabilizer, 0.5-3 parts of anti-deposition agent and 30-100 parts of deionized water; the biological enzyme is a mixture of lipase, protease and cellulase, and the mass ratio of the lipase, the protease and the cellulase is (10-15): (3-8): (1-5); the surfactant is a mixture of a nonionic surfactant and an anionic surfactant, wherein the mass ratio of the nonionic surfactant to the anionic surfactant is (10-15): 1; the anionic area is sulfonate; the sulfonate is alkyl benzene sulfonate; the alkylbenzene sulfonate is sodium heavy alkylbenzene sulfonate; the nonionic surfactant is a C9-11 primary alcohol 8 ethoxylate; the biological enzyme stabilizer is alpha-cyclodextrin; the anti-deposit agent is sorbitol.
2. The preparation method of the high-efficiency biological enzyme cleaning agent as claimed in claim 1, characterized by at least comprising the steps of: mixing biological enzyme, surfactant, biological enzyme stabilizer, anti-deposition agent and deionized water to obtain the product.
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