CN114410714A - Method for preparing chitosan by two-step fermentation of shrimp shells - Google Patents
Method for preparing chitosan by two-step fermentation of shrimp shells Download PDFInfo
- Publication number
- CN114410714A CN114410714A CN202210082738.1A CN202210082738A CN114410714A CN 114410714 A CN114410714 A CN 114410714A CN 202210082738 A CN202210082738 A CN 202210082738A CN 114410714 A CN114410714 A CN 114410714A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- primary
- preparing
- primary fermentation
- chitosan
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
- C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01132—Chitosanase (3.2.1.132)
Abstract
The invention discloses a method for preparing chitosan by two-step shrimp shell fermentation, which comprises the following steps: (1) pretreatment of crayfish shells: drying and crushing the crayfish shell to obtain crayfish shell powder; (2) primary fermentation: preparing a primary fermentation medium by using shrimp shell powder, inoculating lactic acid bacteria and acetic acid bacteria for fermentation, and sterilizing after the fermentation is finished to obtain primary fermentation liquid; (3) and (3) secondary fermentation: performing solid-liquid separation on the primary fermentation liquid, preparing a secondary fermentation culture medium by using the solid isolate, inoculating bacillus subtilis, bacillus licheniformis and rhodobacter to perform fermentation, and sterilizing after the fermentation is finished to obtain secondary fermentation liquid; (4) and (3) secondary fermentation liquor treatment: and (4) performing solid-liquid separation on the secondary fermentation liquor, washing and drying the precipitate to obtain a product. The method has simple operation and high chitosan yield, and is suitable for popularization and application.
Description
Technical Field
The invention belongs to the technical field of recycling of aquatic product processing byproducts, and particularly relates to a method for preparing chitosan by two-step fermentation of shrimp shells.
Background
In the traditional crayfish processing production process, the crayfish shells accounting for about 80 percent of the total weight become wastes, and if the waste crayfish shells are not recycled, resources are wasted and the environment is polluted.
The crayfish shells are rich in chitin, chitosan can be obtained through deacetylation of the chitin, a large amount of free ammonia exists in the molecular structure of the chitosan, the solubility is greatly improved, and the crayfish shells have various physiological functions and can be applied to the aspects of agriculture, medicines, foods, cosmetics, environmental protection and the like, so that the preparation of the chitosan by using the crayfish shells has a wide prospect.
However, when the chitosan is prepared by using the crayfish shells in the traditional method, a large amount of acid and alkali is usually used, the production cost is high, the waste liquid is difficult to treat, and the subsequent treatment process is complex; when the chitosan is prepared by using a microbial fermentation method, the impurity removal efficiency is low, and the deacetylation degree is low.
Therefore, how to provide a shrimp shell treatment method with simple operation and high chitosan yield is an urgent problem to be solved in the field.
Disclosure of Invention
The invention discloses a method for preparing chitosan by two-step shrimp shell fermentation, which can effectively improve the production efficiency of chitosan.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for preparing chitosan by two-step fermentation of shrimp shells comprises the following steps:
(1) pretreatment of crayfish shells:
drying and crushing the crayfish shell to obtain crayfish shell powder;
(2) primary fermentation:
preparing a primary fermentation medium by using shrimp shell powder, inoculating lactic acid bacteria and acetic acid bacteria for fermentation, and sterilizing after the fermentation is finished to obtain primary fermentation liquid;
(3) and (3) secondary fermentation:
performing solid-liquid separation on the primary fermentation liquid, preparing a secondary fermentation culture medium by using the solid isolate, inoculating bacillus subtilis, bacillus licheniformis and rhodobacter to perform fermentation, and sterilizing after the fermentation is finished to obtain secondary fermentation liquid;
(4) and (3) secondary fermentation liquor treatment:
and (4) performing solid-liquid separation on the secondary fermentation liquor, washing and drying the precipitate to obtain a product.
According to the invention, the lactobacillus and the acetic acid bacteria are compounded firstly, and the shrimp shell powder is subjected to primary fermentation, so that the metabolites of the lactobacillus and the acetic acid bacteria can fully dissolve calcium in the shrimp shell, and the shrimp shell powder forms a loose structure, so that the larger particle size is maintained, and the impurity removal and chitosan conversion in the secondary fermentation process are facilitated. The solid isolate after the primary fermentation is subjected to secondary fermentation by compounding bacillus subtilis, bacillus licheniformis and rhodobacter, the conversion of chitosan is realized while impurities are subjected to enzymolysis, and the production efficiency of chitosan can be remarkably improved.
Preferably, in the step (1), the granularity of the shrimp shell powder is 40-80 meshes.
The shrimp shell powder is too fine, so that loss is easily caused in the treatment process; the shrimp shell powder is too coarse, the impurity removal rate is low, and the chitosan conversion efficiency is low; the invention screens the shrimp shell powder with the granularity of 40-80 meshes, facilitates solid-liquid separation, and can fully dissolve impurities.
Preferably, in step (2), the primary fermentation medium consists of:
40-50/L of shrimp shell powder, 5-15g/L of glucose, 5-15g/L of soluble starch, 6-10g/L of yeast extract, 0.3-0.6g/L of sodium acetate, 0.3-0.6g/L of magnesium sulfate, 0.8-1.2g/L of monopotassium phosphate, 0.04-0.06g/L of manganese sulfate, pH5.8-6.2, sterilizing at 115 ℃ for 30min, and then adding 10-15mL/L of absolute ethyl alcohol.
Preferably, in the step (2),
the inoculation amount of the lactobacillus is 1-3% of the volume of the primary fermentation culture medium,
the inoculation amount of the acetic acid bacteria is 1-3% of the volume of the primary fermentation medium.
Preferably, in the step (2),
the primary fermentation temperature is 32-35 ℃, the rotation speed is 120-.
Preferably, in the step (3),
the secondary fermentation medium comprises the following components:
40-50/L of solid isolate, 5-10g/L of glucose, 5-10g/L of soluble starch, 10-15g/L of yeast extract, 0.2-0.5g/L of magnesium sulfate, 0.8-1.2g/L of monopotassium phosphate, 0.04-0.06g/L of manganese sulfate and 30min of sterilization at 115 ℃.
The early-stage rapid growth and the later-stage metabolism of microorganisms are controlled by nutrient components of the culture medium, the synthesis of digestive enzymes such as protease and the like and chitin deacetylase can be promoted, and the enzymolysis of impurities in the shrimp shell powder and the conversion efficiency of chitosan are further improved.
Preferably, in the step (3),
the inoculation amount of the bacillus subtilis is 1-2% of the volume of the primary fermentation culture medium,
the inoculation amount of the bacillus licheniformis is 1-2 percent of the volume of the primary fermentation culture medium,
the inoculation amount of the rhodobacter is 2-4% of the volume of the primary fermentation medium.
Preferably, in the step (3),
the primary fermentation temperature is 34-37 ℃, the rotation speed is 150-.
In conclusion, the preparation method is simple and easy to control, the yield of the prepared chitosan is high, and the efficient recycling of the crayfish shells can be realized.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for preparing chitosan by two-step fermentation of shrimp shells comprises the following steps:
(1) pretreatment of crayfish shells:
drying the crayfish shell, crushing and sieving to obtain 40-80 mesh crayfish shell powder;
(2) primary fermentation:
21) preparing a lactobacillus seed solution:
inoculating Lactobacillus acidophilus ATCC4356 (commercially available) to seed culture medium (glucose 20g/L, yeast extract 5g/L, magnesium sulfate 0.5g/L, potassium dihydrogen phosphate 1g/L, pH6.0, and sterilizing at 115 deg.C for 30 min); culturing at 37 deg.C and 180rpm for 18h to obtain lactobacillus seed solution.
22) Preparing acetic acid bacteria seed liquid:
inoculating acetic acid bacteria ATCC 23768 (commercially available) to seed culture medium (10 g/L glucose, 10g/L yeast extract, 5g/L calcium carbonate, sterilizing at 115 deg.C for 30min, and adding 20mL/L absolute ethanol); culturing at 30 deg.C and 150rpm for 24 hr to obtain acetic acid bacteria seed solution.
23) Preparing a primary fermentation medium:
taking 40g/L shrimp shell powder, 15g/L glucose, 12g/L soluble starch, 8g/L yeast extract, 0.5g/L sodium acetate, 0.5g/L magnesium sulfate, 1g/L monopotassium phosphate, 0.05g/L manganese sulfate, pH5.8-6.2, sterilizing at 115 ℃ for 30min, and adding 10mL/L absolute ethyl alcohol.
24) Inoculating and fermenting:
inoculating 2% lactobacillus seed solution and 2% acetic acid bacteria seed solution according to the volume of the primary fermentation culture medium, fermenting at 34 deg.C and 140rpm for 32 h; sterilizing at 85 deg.C for 15min after fermentation to obtain primary fermentation liquid.
(3) And (3) secondary fermentation:
31) preparing a bacillus subtilis seed solution:
inoculating Bacillus subtilis ATCC 6633 (commercially available) to seed culture medium (glucose 20g/L, peptone 10g/L, beef extract 0.5g/L, sodium chloride 3g/L, sterilizing at 115 deg.C for 30 min); culturing at 36 deg.C and 160rpm for 36h to obtain Bacillus subtilis seed solution.
32) Preparing a bacillus licheniformis seed solution:
inoculating Bacillus licheniformis ATCC 14580 (commercially available) into seed culture medium (sucrose 10g/L, peptone 10g/L, sodium chloride 5g/L, pH7.0, and sterilizing at 115 deg.C for 30 min); culturing at 37 deg.C and 160rpm for 18h to obtain Bacillus licheniformis seed solution.
33) Preparing a rhodobacter seed solution:
inoculating Rhodobacter rhodobacter sp.KCTC 12047 (commercially available) to seed culture medium (beef extract 3g/L, peptone 5g/L, sodium chloride 3g/L, pH7.0, and sterilized at 115 deg.C for 30 min); culturing at 30 deg.C and 160rpm for 24 hr to obtain Rhodobacter xylinum seed solution.
34) Preparing a secondary fermentation culture medium:
performing solid-liquid separation on the primary fermentation liquid, taking 50g/L of solid isolate, 8g/L of glucose, 10g/L of soluble starch, 15g/L of yeast extract, 0.4g/L of magnesium sulfate, 1g/L of monopotassium phosphate and 0.05g/L of manganese sulfate, and sterilizing at 115 ℃ for 30 min.
35) Inoculating and fermenting:
inoculating 1.5% Bacillus subtilis seed solution, 1.5% Bacillus licheniformis seed solution, and 3% Rhodobacterium according to the volume of secondary fermentation medium, fermenting at 36 deg.C and 160rpm for 52 h; sterilizing at 85 deg.C for 15min after fermentation to obtain secondary fermentation liquid.
(4) And (3) secondary fermentation liquor treatment:
and (4) performing solid-liquid separation on the secondary fermentation liquor, washing and drying the precipitate to obtain a product.
Example 2
The particle size of the shrimp shell was adjusted on the basis of example 1, group 1: particle size less than 100 mesh, group 2: the grain diameter is larger than 20 meshes; and detecting the raw material shrimp shell powder, the intermediate product and the final product of each group, including:
measuring ash contents of solid separators obtained after solid-liquid separation of raw material shrimp shell powder and primary fermentation liquid, wherein the mineral removal rate is (shrimp shell powder ash content multiplied by dry weight of the shrimp shell powder-solid separator ash content multiplied by dry weight of the solid separator)/(shrimp shell powder ash content multiplied by dry weight of the shrimp shell powder);
measuring the protein contents of the raw material shrimp shell powder and the final product, wherein the protein weight loss rate (the protein content of the shrimp shell powder, the dry weight of the shrimp shell powder, the protein content of the final product, the dry weight of the final product)/(the protein content of the shrimp shell powder, and the dry weight of the shrimp shell powder);
the chitosan/chitin content in the final product was determined, the chitosan/chitin yield ═ [ (chitosan content of the final product + chitin content of the final product) × weight of the final product ]/weight of shrimp shell meal, the chitosan/chitin recovery ═ [ (chitosan content of the final product + chitin content of the final product) × weight of the final product ]/amount of chitin converted to chitosan in shrimp shell meal, the amount of chitosan was calculated as more than 55% N-acetyl deacetylation.
The deacetylation degree of the chitosan/chitin is determined by using 4000-500cm samples of the chitosan/chitin by Fourier infrared spectroscopy-1The degree of deacetylation was 1-1.15 (A1655/A3450), where A1655 and A3450 each refer to a sample at 1655cm-1And 3450cm-1The absorbance value of (a).
The results are shown in Table 1.
TABLE 1
Example 3
The fermentation process was adjusted on the basis of example 1:
group 3: only carrying out primary fermentation, carrying out solid-liquid separation on primary fermentation liquor, washing precipitate with water, and drying to obtain a product;
group 4: the secondary fermentation is not inoculated with the rhodobacter;
group 5: inoculating 1% of lactobacillus seed solution, 1% of acetic acid bacteria seed solution, 1% of bacillus subtilis seed solution, 1% of bacillus licheniformis seed solution and 2% of rhodobacter xylinum seed solution according to the volume of a primary fermentation culture medium during primary fermentation, and fermenting at 34 ℃ and 140rpm for 32 hours; sterilizing at 85 deg.C for 15min after fermentation to obtain primary fermentation liquid, separating solid and liquid, washing precipitate with water, and drying to obtain product.
Group 6: and (3) directly carrying out solid-liquid separation without sterilization after primary fermentation, and preparing a secondary fermentation culture medium.
Group 7: replacing the secondary fermentation medium with: 50g/L of solid isolate, 15g/L of glucose, 15g/L of soluble starch, 15g/L of yeast extract, 0.6g/L of magnesium sulfate, 1g/L of monopotassium phosphate and 0.05g/L of manganese sulfate, and sterilizing at 115 ℃ for 30 min.
The chitosan content of the shrimp shell powder and the chitosan content of the final product of each group of raw materials are detected, and the chitosan yield and recovery rate are calculated, and the results are shown in table 2.
TABLE 2
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to the above-described embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. A method for preparing chitosan by two-step fermentation of shrimp shells is characterized by comprising the following steps:
(1) pretreatment of crayfish shells:
drying and crushing the crayfish shell to obtain crayfish shell powder;
(2) primary fermentation:
preparing a primary fermentation medium by using shrimp shell powder, inoculating lactic acid bacteria and acetic acid bacteria for fermentation, and sterilizing after the fermentation is finished to obtain primary fermentation liquid;
(3) and (3) secondary fermentation:
performing solid-liquid separation on the primary fermentation liquid, preparing a secondary fermentation culture medium by using the solid isolate, inoculating bacillus subtilis, bacillus licheniformis and rhodobacter to perform fermentation, and sterilizing after the fermentation is finished to obtain secondary fermentation liquid;
(4) and (3) secondary fermentation liquor treatment:
and (4) performing solid-liquid separation on the secondary fermentation liquor, washing and drying the precipitate to obtain a product.
2. The method for preparing chitosan by two-step fermentation of shrimp shells according to claim 1,
in the step (1), the granularity of the shrimp shell powder is 20-120 meshes.
3. The method for preparing chitosan by two-step fermentation of shrimp shells according to claim 1,
in the step (2), the primary fermentation medium comprises the following components:
40-50/L of shrimp shell powder, 5-15g/L of glucose, 5-15g/L of soluble starch, 6-10g/L of yeast extract, 0.3-0.6g/L of sodium acetate, 0.3-0.6g/L of magnesium sulfate, 0.8-1.2g/L of monopotassium phosphate, 0.04-0.06g/L of manganese sulfate, pH5.8-6.2, sterilizing at 115 ℃ for 30min, and then adding 10-15mL/L of absolute ethyl alcohol.
4. The method for preparing chitosan by two-step fermentation of shrimp shells according to claim 1,
in the step (2),
the inoculation amount of the lactobacillus is 1-3% of the volume of the primary fermentation culture medium,
the inoculation amount of the acetic acid bacteria is 1-3% of the volume of the primary fermentation medium.
5. The method for preparing chitosan by two-step fermentation of shrimp shells according to claim 1,
in the step (2),
the primary fermentation temperature is 32-35 ℃, the rotation speed is 120-.
6. The method for preparing chitosan by two-step fermentation of shrimp shells according to claim 1,
in the step (3), the step (c),
the secondary fermentation medium comprises the following components:
40-50/L of solid isolate, 5-10g/L of glucose, 5-10g/L of soluble starch, 10-15g/L of yeast extract, 0.2-0.5g/L of magnesium sulfate, 0.8-1.2g/L of monopotassium phosphate, 0.04-0.06g/L of manganese sulfate and 30min of sterilization at 115 ℃.
7. The method for preparing chitosan by two-step fermentation of shrimp shells according to claim 1,
in the step (3), the step (c),
the inoculation amount of the bacillus subtilis is 1-2% of the volume of the primary fermentation culture medium,
the inoculation amount of the bacillus licheniformis is 1-2 percent of the volume of the primary fermentation culture medium,
the inoculation amount of the rhodobacter is 2-4% of the volume of the primary fermentation medium.
8. The method for preparing chitosan by two-step fermentation of shrimp shells according to claim 1,
in the step (3), the step (c),
the primary fermentation temperature is 34-37 ℃, the rotation speed is 150-.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210082738.1A CN114410714B (en) | 2022-01-15 | 2022-01-15 | Method for preparing chitosan by two-step fermentation of shrimp shells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210082738.1A CN114410714B (en) | 2022-01-15 | 2022-01-15 | Method for preparing chitosan by two-step fermentation of shrimp shells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114410714A true CN114410714A (en) | 2022-04-29 |
| CN114410714B CN114410714B (en) | 2023-10-13 |
Family
ID=81276598
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210082738.1A Active CN114410714B (en) | 2022-01-15 | 2022-01-15 | Method for preparing chitosan by two-step fermentation of shrimp shells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114410714B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101144097A (en) * | 2007-09-18 | 2008-03-19 | 重庆百奥帝克微生态科技有限公司 | Method for preparing chitin and its chitosan and chitosan oligosaccharide |
| CN110643668A (en) * | 2019-10-29 | 2020-01-03 | 海南大学 | Method for extracting chitin from shrimp shells by utilizing microbial fermentation |
| KR102150046B1 (en) * | 2019-12-06 | 2020-08-31 | 주식회사 건강을담은 | Manufacturing method of chitosan solution and chitosan solution manufactured by the method |
-
2022
- 2022-01-15 CN CN202210082738.1A patent/CN114410714B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101144097A (en) * | 2007-09-18 | 2008-03-19 | 重庆百奥帝克微生态科技有限公司 | Method for preparing chitin and its chitosan and chitosan oligosaccharide |
| CN110643668A (en) * | 2019-10-29 | 2020-01-03 | 海南大学 | Method for extracting chitin from shrimp shells by utilizing microbial fermentation |
| KR102150046B1 (en) * | 2019-12-06 | 2020-08-31 | 주식회사 건강을담은 | Manufacturing method of chitosan solution and chitosan solution manufactured by the method |
Non-Patent Citations (2)
| Title |
|---|
| 母运龙;张?;张龙翼;柯欢;郭添荣;李慧;: "小龙虾虾壳副产物制备甲壳素的研究进展", 四川农业科技, no. 02 * |
| 顾真荣, 马承铸, 韩长安: "产几丁质酶芽孢杆菌的筛选鉴定和酶活力测定", 上海农业学报, no. 03 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114410714B (en) | 2023-10-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105039193A (en) | Strain and method for producing glucosamine through microorganism fermentation | |
| JPH05199892A (en) | Production of chitosan | |
| CN101423855B (en) | Method for preparing polysaccharide by using lucidum strain fermented laminaria leftover | |
| CN102978134A (en) | Lactobacillus and method for producing D-lactic acid by fermenting using lactobacillus | |
| CN109628513B (en) | Amino acid fermentation medium and preparation method thereof | |
| CN106754410A (en) | A kind of method for producing the bacterial strain of pulullan polysaccharide and utilizing its fermenting and producing pulullan polysaccharide | |
| CN101676399B (en) | Technological method for producing xylitol by biotransformation of corncobs | |
| CN102766658B (en) | Production process of Jew's ear melanin by fermentation and products of Jew's ear melanin | |
| CN105219661B (en) | The special strain therefore of synthesis of oligonucleotides galactolipin and method with its synthesis of oligonucleotides galactolipin | |
| CN110938666B (en) | Preparation method of microbial chitosan | |
| CN101735331B (en) | Production process for extracting lily polysaccharides through fermentation method and product thereof | |
| CN102703558B (en) | Method for hydroxylating dehydroisoandrosterone by using colletotrichumlini | |
| CN114410714B (en) | Method for preparing chitosan by two-step fermentation of shrimp shells | |
| CN103361394A (en) | Method for preparing 9alpha-hydroxide-androstenedione by utilizing microbial conversion | |
| CN108925747B (en) | Functional protein prepared from aquatic protein and yeast and preparation method thereof | |
| CN102816751B (en) | High-activity chitosanase and preparation method thereof | |
| CN109136314A (en) | The method for synthesizing 2 '-deoxidation -2- amino adenosines using Michigan's Klebsiella | |
| CN114574403A (en) | Method for preparing thermophilic thermus strain fermentation product by utilizing rice bran enzyme hydrolysate | |
| CN114369627A (en) | Method for producing lactic acid by promoting co-fermentation of kitchen waste and mushroom residue by aspergillus niger | |
| CN101676397B (en) | Method for preparing alcohol by utilizing potato materials | |
| CN110946208A (en) | Clean production method for producing chitin and feeding composite microecological preparation by one-step fermentation of shrimp and crab shells | |
| CN114507697B (en) | Synthesis method of degradable biological material polyglutamic acid | |
| CN111925421B (en) | Method for extracting pneumocandin B0 from fermentation liquor and application | |
| CN107164286B (en) | Microbial strain and breeding method and application thereof | |
| JP2004254542A (en) | Method for producing lactic acid from waste food |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |