CN114410650A - Rice salt sensitive mutant gene SS2, mutant SS2 and application - Google Patents
Rice salt sensitive mutant gene SS2, mutant SS2 and application Download PDFInfo
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Abstract
The invention belongs to the technical field of rice salt sensitive mutation, and particularly relates to a rice salt sensitive mutant gene SS2, a mutant SS2 and application. The nucleic acid sequence of the mutant gene SS2 is shown in a sequence table SEQ ID NO.1, and the amino acid sequence is shown in a sequence table SEQ ID NO. 2. The rice salt sensitive mutant gene SS2 is a mutant gene in which a single base substitution (C mutation is T) occurs in the No.2 exon (259 base of a gene coding region) of rice LOC _ Os10g41780, so that the 87 th glutamine (Gln) of a coding protein sequence is mutated into a termination code, the distribution of sugar from source leaves to sink roots under salt stress is inhibited, the SS2 salt sensitivity is caused, the plant growth is inhibited by reducing the supply of carbohydrates such as sucrose and the like, the SS2 salt stress adaptability is reduced, and the rice salt sensitive mutant gene can be applied to the research on the aspects of rice growth and salt stress response.
Description
Technical Field
The invention belongs to the technical field of rice salt sensitive mutation, and particularly relates to a rice salt sensitive mutant gene SS2, a mutant SS2 and application.
Background
Salinity severely limits crop yield, particularly for crops grown in irrigated conditions or in coastal lowlands that are susceptible to seawater erosion. Currently, over 20% of the arable land worldwide is affected by soil salinity. By 2050, almost half of the arable land is predicted to be in a salinized state due to rapid changes in global climate. In monocotyledonous crops, rice is sensitive to salt stress, and the effects of soil salt accumulation include osmotic stress, ion toxicity and nutrient deficiency, which ultimately leads to growth inhibition and yield reduction. Osmoregulation is an important mechanism for rice to adapt to salt stress by regulating and controlling inorganic ions (Na) in vivo+、K+、Cl-) And accumulation of organic solutes (soluble carbohydrates, amino acids, etc.), maintaining water uptake and cell turgor in high salt stress. At the molecular level, gene expression involved in different metabolic pathways is regulated to control salt uptake, maintain ion homeostasis, and the growth rate of cells.
Carbohydrates (mainly sucrose) are produced by photosynthesis in the source organs and transported in the plant body through vascular tissues. As a nutrient, synthesis, storage and long-distance transport of sugars to the sink organs (e.g., roots, flowers and seeds) are essential for the maintenance of plant growth and development. As an important osmoregulation substance, more sugar is accumulated in the vacuole of stressed plants to generate higher turgor pressure. Under salt stress, the content of glucose, fructose, sucrose and levan in the high-tolerance wheat is far higher than that of the sensitive wheat. Populus diversifolia responds to high salt stress by increasing soluble total sugars in young leaves and decreasing in mature leaves. In maize, plants inoculated with arbuscular mycorrhizal fungi are significantly more salt tolerant than non-planted plants due to their higher soluble sugar content in vivo.
Although researches have found that sugar metabolism changes are important physiological characteristics of plants in response to abiotic stress, the relation research of sugar transport and distribution and rice salt tolerance is rarely reported, and key functional genes and signal transduction pathways influencing rice response to high-salt stress by regulating sugar transport are not clear.
Disclosure of Invention
In view of the above problems, the present invention aims to provide a rice salt-sensitive mutant gene SS2, a mutant SS2 and applications thereof, wherein the SS2 mutation can inhibit long-distance transportation of sucrose from a source to a bank and plant growth, and further reduce the salt tolerance of rice.
The technical content of the invention is as follows:
the invention provides a rice salt sensitive mutant gene SS2, the nucleic acid sequence of which is shown in a sequence table SEQ ID NO.1, and the amino acid sequence of which is shown in a sequence table SEQ ID NO. 2;
the rice salt sensitive mutant gene SS2 is a mutant gene of a termination code, wherein single base substitution (C mutation is T) is carried out on the No.2 exon (259 base of a gene coding region) of rice LOC _ Os10g41780, so that the 87 th glutamine (Gln) of a coding protein sequence is mutated;
the rice salt sensitive mutant gene SS2 can regulate and control sugar running in vivo.
The invention also provides a rice salt sensitive mutant gene SS2, which is applied to the research of rice growth and salt stress response.
The invention also provides a rice salt sensitive mutant SS2, which is a plant mutant with a mutant gene SS2, wherein the rice salt sensitive mutant SS2 can be used as an indicator plant of salinized soil or environment and is planted in a place with higher soil salinization degree, the soil salinity degree can be visually seen, the salt accumulation content of the overground part in the mutant is high, and the subsequent harvesting is expected to achieve the effect of removing the salt in the soil;
the rice salt sensitive mutant ss2 is obtained by adopting Wuyujing No. 7, performing mutagenesis by Ethyl Methanesulfonate (EMS), salt stress treatment and screening.
The invention has the following beneficial effects:
the rice salt sensitive mutant gene SS2 is an influence gene of rice salt tolerance, leads to SS2 salt sensitivity by inhibiting the distribution of sugar from source leaves to stock roots under the salt stress, inhibits the growth of plants by reducing the supply of carbohydrates such as sucrose and the like, and reduces the salt stress adaptability of SS 2;
the invention screens and identifies the rice salt-sensitive mutant, clones the map to the gene SS2, and defines the mutual relation and action mechanism between the participated sugar metabolism and the rice salt tolerance through phenotype, physiology and heredity analysis, perfects the molecular regulation network of rice responding to high salt stress, further develops the molecular design breeding with resistance as the target, and creates new salt-tolerant stable-yield germplasm for the sustainable development of agricultural production.
Drawings
FIG. 1 shows the growth of rice under normal and salt stress conditions at the seedling stage of ss2 mutant according to the present invention;
FIG. 2 is a diagram of soil culture salt-perfusion growth of ss2 mutant at the tillering stage;
FIG. 3 shows the physiological response of ss2 mutant and WT at tillering stage to salt stress;
FIG. 4 shows the difference between the sucrose transport of ss2 mutant and WT under salt stress;
FIG. 5 shows the effect of SS2 mutation on the expression of sugar transport-related genes in WT and SS2 source leaves;
FIG. 6 is a schematic map cloning of SS 2;
FIG. 7 is a graph of analysis of growth differences between WT and complementary transgenic lines under salt stress.
Detailed Description
The present invention is described in further detail in the following description of specific embodiments and the accompanying drawings, it is to be understood that these embodiments are merely illustrative of the present invention and are not intended to limit the scope of the invention, which is defined by the appended claims, and modifications thereof by those skilled in the art after reading this disclosure that are equivalent to the above described embodiments.
All the raw materials and reagents of the invention are conventional market raw materials and reagents unless otherwise specified.
Examples
Acquisition of mutant ss2 plants:
1) and (3) mutagenesis treatment: selecting Wuyujing No. 7 rice seeds, and respectively adopting Ethyl Methanesulfonate (EMS) and sodium azide (NaN3) for mutagenesis, wherein the method specifically comprises the following steps:
EMS processing:
soaking Wuyujing No. 7 rice seed at 28 deg.C for 24 hr, drying, treating with EMS solutions of different concentrations at 28 deg.C for 20 hr, respectively, the EMS concentrations are 1.5% (v/v), 1.0%, 0.5% and 0%, repeating twice, washing with tap water for 5 hr, and performing germination in light incubator (14 hr illumination [30 deg.C ]]10h darkness [25 deg.C]Photoperiod, 100. mu. mol m-2s-1Light intensity), germination was counted after 4 days, and the results are shown below:
TABLE 1 seed germination (%)
NaN3 treatment:
first, a solution, 1mM KH, is prepared2PO4To 100mL was added about 12mL of H3PO4Adding NaN3 into a buffer solution with the final pH value of 3.0 as a reference, dividing the Wuyujing No. 7 rice seeds into two parts, soaking one part of the seeds at room temperature for 24 hours, not soaking the other part of the seeds in advance, respectively adding the soaked seeds and the seeds which are not soaked into NaN3 solutions with the concentrations of 0.5mM, 1mM and 2mM for soaking for 12 hours, then washing the seeds with natural water for 2 hours, accelerating germination in illumination culture, detecting the germination rate after 4 days, and finding that the germination rate is close to 0 under several concentrations.
As can be seen from the above, in view of the fact that the seeds treated by NaN3 do not sprout, the treatment method is abandoned, EMS is selected, Wuyujing No. 7 rice seeds with the concentration of 1.5 percent are selected, 2 jin of seeds are treated together, the seeds are stirred once during soaking to prevent the solution from being uneven, and the seeds are sowed in Fuyang test base of China Rice research institute, which is marked as M0Generation, collecting seeds by single plant after maturation, wherein each single plant is provided with a serial number for the research of Chinese riceSowing in the hilly water test base, marked as M1And thirdly, investigating the phenotype, checking the segregation ratio, and harvesting seeds by single plants until the subsequent phenotype is stable and has no segregation, wherein the EMS mutant screened under the salt stress is M12And (4) generation.
2) Salt stress treatment: the above-mentioned mutant plant M12Placing in a climatic chamber, lighting for 14 hr (30 deg.C), darkness for 10 hr (25 deg.C), maintaining relative humidity at 70%, and replacing nutrient solution with 1.25mM NH4NO3,0.3mM KH2PO4，0.35mM K2SO4，1mM CaCl2·2H2O,1mM MgSO4·7H2O,0.5mM Na2SiO3,20μM NaFeEDTA,20μM H3BO3,9μM MnCl2·4H2O,0.32μM CuSO4·5H2O,0.77μM ZnSO4·7H2O and 0.39. mu.M Na2MoO4·2H2O,pH 5.5；
The salt stress response mutant is screened and the salt tolerance of the genetic complementary transgenic material is identified, 10-day-old seedlings growing under the culture condition are transferred into a nutrient solution with the final concentration of 100mM NaCl to be treated for 4 days, the growth vigor of the seedlings, the withering degree of leaves and the etiolation areas of the leaf tips and the leaf margins in the treatment period are observed, the rice with high salt tolerance is high, the growth inhibition degree of the seedlings and the damage degree of the leaves are low, the high salt stress hypersensitive mutant material ss2(salinity sensitive 2) is finally screened and identified, the physiological parameter and the gene expression mode of the high salt stress hypersensitive mutant material are detected, and the test method and the result are as follows.
1.Na+And K+Determination of concentration
Ss2 and WT plants were placed in 0.1mM CaSO4Rinsing in solution for 5min, separating aerial part and root system, drying at 105 deg.C for 30min, drying at 70 deg.C to constant weight, grinding into powder, and weighing, reference paper (Chen G, Liu C, Gao Z, et al].Environmental&Experimental Botany,2018,147: 147-156)Digestion, cooling and diluting to 50mL with distilled water. Determination of Na in the digestate by Optima 2100DV ICP emission spectrometer (Perkinelmer, USA)+And K+And (4) concentration.
2. Measurement of chlorophyll content
A method of a reference paper (Chen G, Liu C, Gao Z, et al. variation in the absendance of OsHAK1 transcript undersides the differential diagnosis of leaves of an index and a cosmetic edge culture [ J ]. Frontiers in Plant Science,2017,8:2216.), harvesting of Plant leaves, weighing and extraction with 95% (v/v) ethanol solution, recording the absorbance (A) of the extract at 663 and 645nm using a spectrophotometer (Shimadzu UV2400, Japan), the calculation formula of the total chlorophyll content being 8.02A663+20.21A 645.
3. Determination of the Net photosynthetic Rate (Pn)
The method of the reference paper (Chen G, Liu C, Gao Z, et al. variation in the absendance of OsHAK1 transcript underscales of the differential diagnosis of the present invention of an index and a cosmetic edge tolerance [ J ]. Frontiers in Plant Science,2017,8:2216.) was used to determine 9 am using a Li-COR6400 portable photosynthesizer (Li-COR, USA): 00 to 11: 00 net photosynthetic rate of rice leaves.
4. Hydrogen peroxide (H)2O2) And Malondialdehyde (MDA) content determination
Harvesting aerial parts of the plants after stress treatment, grinding into powder with liquid nitrogen, and using H2O2And MDA kit (Nami Biotechnology Co., Ltd., Suzhou Kogyo) were extracted and referred to in a paper (Chen G, Liu C, Gao Z, et al. OsHAK1, a high-affinity site transporter, localization regulations to gravity site in rice [ J]Frontiers in Plant Science,2017,8:1885.) method.
5. Determination of sucrose content
Leaves and roots of plants were harvested after stress treatment, ground to powder in liquid nitrogen, and assayed using a sucrose kit (Nami Biotechnology Co., Ltd., Suzhou) extracted and referenced in the paper (Chen G, Hu J, Dong L, et al. the tolerance of saline in rice requirements of the paper of functional copy of FLN2[ J ]. biomodules, 2020,10(1): 17.).
6. Determination of Sucrose Efflux Rate (SER)
The EDTA method of the reference paper (Chen G, Zhang Y, Ruan B, et al, OsHAK1 controls the genetic improvement and the university of rice by fact effect on porous-medium metabolism [ J ] Plant Science,2018,274:261 and 270.) collects phloem exudate from source leaves, and the cut ends of the leaves are immediately immersed in 20mL of 30mM EDTA solution (pH 7.0) and in the dark for 15 min. To avoid the effect of xylem exudate, the first EDTA solution was discarded, and the leaves were washed and transferred to 10mL of 30mM EDTA solution, which was placed in a high relative humidity closed dark room throughout the collection process. After 4h, the sucrose concentration in the collection was determined using a sucrose kit.
7. Real-time fluorescent quantitative PCR (qRT-PCR)
The procedure of the reference paper (Chen G, Hu Q, Luo L, et al. Rice site promoter OsHAK1 is the addressing for main assessing the site-mediated growth and functions in salt tolerance over low and high site tolerance sequences [ J ]. Plant, Cell & Environment,2015,38(12):2747-2765.) extracts the RNA of Plant source leaves under normal and salt stress conditions, respectively. Rice UBQ5(LOC _ Os01G22490) is an internal reference gene, and the relative expression abundance is determined by an algorithm in a reference paper (Chen G, Wu C, He L, et al. knocking out the gene RLS1 indexes hypersensory to oxidative stress and expression leaf sensitivity in rice [ J ]. International Journal of Molecular Sciences,2018,19(10): 2853.).
The sequences of various quantitative primers used in the experiment are shown in Table 2, and the sequences are respectively SEQ ID NO. 3-SEQ ID NO.12 in the sequence table in sequence (from left to right and from top to bottom).
TABLE 2 primers for fluorescent quantitative PCR
8. Gene mapping and candidate gene determination
According to the method of the reference paper (Chen G, Wu C, He L, et al. bundling out the gene RLS1 expressed in terms of specificity to oxidative stress and expression leave sensitivity in rice [ J ]. International Journal of Molecular Sciences,2018,19(10):2853.), DNA of parent and population is extracted by CTAB method, SSR marker primers distributed on 12 chromosomes of rice existing in laboratory are used, linkage sites are preliminarily determined by pool mixing method, the sequences of Nippon rice and indica rice 9311 in the initial positioning interval are subjected to differential analysis, encrypted primers are designed by Primer Premier 5 software, further gene positioning is carried out, the sequences of related primers are shown in Table 3, and the sequences are SEQ ID NO. 13-SEQ ID NO.44 in the sequence table respectively in sequence (from left to right and from top to bottom).
According to a GRAMENE website (http:// ensembl. GRAMENE. org /), all coding frames (ORFs) in a positioning interval are analyzed, primers are designed to sequence wild Wuyujing No. 7 and ss2 respectively, DNAstar software sequence comparison is used for determining candidate genes and mutation sites, and a genetic map is constructed.
TABLE 3 marker primers for Gene mapping
9. Construction of complementary transgenic Material
A6174 bp fragment containing an SS2 coding region and upstream and downstream sequences is amplified by using wild type Wuyun japonica No. 7 genomic DNA as a template, the amplified fragment is connected to a pCAMBIA1300 vector by a GBclone seamless cloning kit (Suzhou Shenzhou Gene Co., Ltd.) after purification, a correctly sequenced plasmid is introduced into Agrobacterium EHA105 by an electric shock method, a mature embryo callus of a SS2 mutant is infected, and the method is referred to from a paper (Chen G, Hu J, Lian J, et al. functional characterization of OsHAK1 promoter in response to an embryonic/moisture deletion in transformation [ J ]. Plant Growth Regulation,2019,88(3): 241. 251.).
According to the above test method, the following results were obtained:
1. growth of plants under salt stress conditions
As shown in FIG. 1, FIG. 1A shows that under normal culture conditions, the growth vigor of ss2 and WT at seedling stage is substantially the same; under the condition of salt stress, the growth of rice seedlings is kept all the time, the inhibition degrees are obviously different, the ss2 plants are severely wilted, the leaves are chlorosis, and the WT only shows slight wilting and leaf tip wilting;
FIGS. 1B and 1C show that, under the salt stress treatment conditions, the overground growth of WT and ss2 was inhibited, and the plant height and fresh weight of the salt-stressed ss2 seedlings were significantly higher than that of WT; wherein, the fresh weight of the overground part of the ss2 mutant is only 50% of that of the WT under the treatment of 100mM NaCl;
FIGS. 1D and 1E show the aerial Na of ss2 in the Normal culture Environment+The concentration and the sodium-potassium concentration ratio have no obvious difference with WT, and are respectively 40 percent and 45 percent higher than wild type under salt stress treatment;
therefore, the screened mutant ss2 is hypersensitive to salt stress, can be used as an indicator plant of salinized soil or environment, is planted in a place with higher salinization degree of soil, can visually see the salt damage degree, has more salt accumulation in the mutant, is particularly on the ground, and is harvested subsequently, so that the effect of removing the salt in the soil is expected to be achieved.
In order to monitor the phenotype of mutant responding to salt stress more intuitively, ss2 is planted in a soil environment, a barrel culture experiment is adopted, 20kg of rice field soil is filled in each barrel, 3 seedlings are planted in each barrel, NaCl is applied to the barrels in batches when the rice grows to 5 weeks of seedling age, 1.5g of NaCl/kg of soil is finally reached, after 3 weeks of treatment, as shown in figure 2, the mature leaves of the ss2 mutant are withered and yellowed in a large area, and soil salinization is indicated visually.
SS2 mutation to reduce the salt stress tolerance of rice in tillering stage
To further reveal the role of SS2 in the rice salt stress response, the 6-week-old SS2 mutant and WT were incubated in normal and 150mM NaCl-containing nutrient solutions for 4 days, as shown in fig. 3A, the total chlorophyll content of the former was found to be lower than that of the latter, in NaCl-treated plants, the chlorophyll content of SS2 was significantly reduced, while WT remained relatively stable, compared to the leaves of SS2 and WT plants grown under normal conditions; figure 3B shows that the determination of non-stressed plant leaf Pn also revealed significant differences between the mutant and WT, with the differences between ss2 and wild type Pn being magnified in salt stressed plants; these results indicate that the SS2 mutation reduces the salt tolerance of rice plants at the tillering stage.
Effect of SS2 mutation on sugar transport in Rice
Since the total chlorophyll content and Pn are closely related to the production of photosynthetic assimilates in plants, SS2 was postulated to alter the sensitivity of rice to salt stress by regulating sugar metabolism. To test this hypothesis, the sucrose content in the leaves of the source organ was first determined, as shown in FIG. 4, FIG. 4A showing that under normal conditions the sucrose content of ss2 was found to be 15% higher than WT, whereas in the leaves of NaCl-treated plants this increased to 24%; in contrast, fig. 4B shows that the SS2 mutation resulted in a significant decrease in sucrose content in the coorgan roots under non-stressed conditions, and that NaCl treatment was relatively less effective on WT roots, resulting in a sucrose content in stressed SS2 roots that was about 16% lower than WT; figure 4C shows that, consistent with the trend of sucrose content in roots, under normal conditions, the sucrose export rate of the source leaves of SS2 plants was less than WT 23%, which increased to 44% under salt stress, indicating that the SS2 mutation significantly inhibited the loading of sucrose into phloem in the source organ.
The genes involved in sugar transport in the above WT and ss2 source leaves were analyzed for differential expression, and as shown in FIG. 5, 4 genes tested were all down-regulated under normal conditions. In addition, salt stress inhibits the expression of OsSUT4(Os02g0827200), OsSWEET11(Os08g0535200), OsSWEET14(Os11g0508600) and OsMT (Os04g0602400) in WT and SS2, but the decrease amplitude of SS2 is greater than that of WT, so that the difference is more remarkable, and the fact that the expression level of sugar transport related genes is remarkably reduced is shown, and is an important reason for inhibiting the sucrose transport from a source to a reservoir by SS2 mutation.
Fine localization and complementation verification of the SS2 Gene
As shown in FIG. 6A, F was prepared from indica rice variety Nanjing No. 6 and ss22Segregating the population, and initially mapping the target gene on chromosome 10Between markers SS11 and SS 32;
as shown in FIG. 6B, InDel marker was designed based on the sequence differences between Nipponbare and 9311, and finally the target gene was finely mapped to the 55kb range between markers SS23 and SS24 on BAC clone OSJNBa0057L 21;
as shown in FIG. 6C, 11 predicted genes were found in this interval, and sequencing revealed that single base substitution (C to T) occurred in exon 2 of LOC _ Os10g41780 (259 th base of gene coding region) in ss2, the coding nucleic acid sequence thereof is shown in SEQ ID No.1 of the sequence Listing, and glutamine (Gln) at position 87 of the coding protein sequence is mutated to a stop codon, resulting in premature termination of translation, and the amino acid sequence thereof is shown in SEQ ID No.2 of the sequence Listing.
As shown in FIG. 7, it was verified whether the generation of the salt-sensitive trait ss2 was caused by mutation of the candidate gene by constructing complementary transgenic material (COM), and the results showed that the phenotype, the above-ground and root dry weights, H, of the transgenic line under the salt stress condition2O2And the MDA content is not different from that of the wild type, the salt tolerance of the plant is recovered to be normal, and the generation of the ss2 salt sensitive character is caused by the mutation of the candidate gene.
Sequence listing
<110> research institute of agricultural quality standard and monitoring technology of Guangdong province academy of agricultural sciences
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tgtgctcaca gagcttgccc tcttcatctt ggctcagtta atgagggcag aatccaatgc 840
ccttaccatg gttgggagta ttcaactgat ggaaaatgtg agaaaatgcc atccacaaag 900
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<213> Artificial Sequence
<400> 5
<210> 6
<211> 19
<212> DNA
<213> Artificial Sequence
<400> 6
cgtgaggagc gagagctga 19
<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 7
<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 8
<210> 9
<211> 21
<212> DNA
<213> Artificial Sequence
<400> 9
ttcccaacgt gctgggcttc t 21
<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 10
<210> 11
<211> 18
<212> DNA
<213> Artificial Sequence
<400> 11
gctgccaggc aggaagct 18
<210> 12
<211> 21
<212> DNA
<213> Artificial Sequence
<400> 12
ggttccagtt tcaccacgac a 21
<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 13
<210> 14
<211> 20
<212> DNA
<213> artificial sequence
<400> 14
catgctaaca tgaggtgatc 20
<210> 15
<211> 20
<212> DNA
<213> artificial sequence
<400> 15
<210> 16
<211> 22
<212> DNA
<213> artificial sequence
<400> 16
ggtgggcctc gattgtaagt ag 22
<210> 17
<211> 20
<212> DNA
<213> artificial sequence
<400> 17
ttggctcttc tccttagtat 20
<210> 18
<211> 20
<212> DNA
<213> artificial sequence
<400> 18
catttgtatc ttgtgaacgt 20
<210> 19
<211> 18
<212> DNA
<213> artificial sequence
<400> 19
ccaatgtttg ctccagat 18
<210> 20
<211> 18
<212> DNA
<213> artificial sequence
<400> 20
ttcaatgacc cacgtccc 18
<210> 21
<211> 18
<212> DNA
<213> artificial sequence
<400> 21
tggttttcct tgttgctg 18
<210> 22
<211> 18
<212> DNA
<213> artificial sequence
<400> 22
gcttgcggct ctgcttac 18
<210> 23
<211> 20
<212> DNA
<213> artificial sequence
<400> 23
<210> 24
<211> 20
<212> DNA
<213> artificial sequence
<400> 24
<210> 25
<211> 20
<212> DNA
<213> artificial sequence
<400> 25
<210> 26
<211> 20
<212> DNA
<213> artificial sequence
<400> 26
<210> 27
<211> 20
<212> DNA
<213> artificial sequence
<400> 27
ggaggagttc atttgaggcg 20
<210> 28
<211> 20
<212> DNA
<213> artificial sequence
<400> 28
<210> 29
<211> 21
<212> DNA
<213> artificial sequence
<400> 29
atacctcctt gtattcgcac t 21
<210> 30
<211> 20
<212> DNA
<213> artificial sequence
<400> 30
<210> 31
<211> 20
<212> DNA
<213> artificial sequence
<400> 31
agcgtgaatc taatagcact 20
<210> 32
<211> 20
<212> DNA
<213> artificial sequence
<400> 32
<210> 33
<211> 21
<212> DNA
<213> artificial sequence
<400> 33
ttgtaaccgt cgatttcgtt c 21
<210> 34
<211> 21
<212> DNA
<213> artificial sequence
<400> 34
ccgctccgtc actctactac c 21
<210> 35
<211> 20
<212> DNA
<213> artificial sequence
<400> 35
<210> 36
<211> 20
<212> DNA
<213> artificial sequence
<400> 36
<210> 37
<211> 20
<212> DNA
<213> artificial sequence
<400> 37
<210> 38
<211> 20
<212> DNA
<213> artificial sequence
<400> 38
<210> 39
<211> 23
<212> DNA
<213> artificial sequence
<400> 39
ttggcgatta atgatccggg aac 23
<210> 40
<211> 20
<212> DNA
<213> artificial sequence
<400> 40
<210> 41
<211> 20
<212> DNA
<213> artificial sequence
<400> 41
<210> 42
<211> 24
<212> DNA
<213> artificial sequence
<400> 42
gtagtataaa ttgtaatagc tcaa 24
<210> 43
<211> 20
<212> DNA
<213> artificial sequence
<400> 43
<210> 44
<211> 20
<212> DNA
<213> artificial sequence
<400> 44
Claims (4)
1. A paddy rice salt sensitive mutant gene SS2 is characterized in that the mutant gene SS2 has a nucleic acid sequence shown in a sequence table SEQ ID NO.1 and an amino acid sequence shown in a sequence table SEQ ID NO. 2.
2. A rice salt sensitive mutant gene SS2 is applied to the research of rice growth and salt stress response.
3. A rice salt-sensitive mutant SS2, wherein mutant SS2 is plant mutant with mutant gene SS2 as claimed in claim 1.
4. A rice salt-sensitive mutant ss2 as claimed in claim 3 for use as an indicator plant in salinized soil or environment.
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| CN202210003113.1A CN114410650B (en) | 2022-01-04 | 2022-01-04 | Rice salt sensitive mutant gene SS2, mutant SS2 and application |
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| CN114410650A true CN114410650A (en) | 2022-04-29 |
| CN114410650B CN114410650B (en) | 2022-10-18 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115851760A (en) * | 2022-09-01 | 2023-03-28 | 广东省农业科学院农业质量标准与监测技术研究所 | Rice high-salt-sensitivity mutant gene SS3, mutant SS3 and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060003354A1 (en) * | 2004-05-03 | 2006-01-05 | Krantz Ian D | Methods and compositions for the diagnosis of Cornelia de Lange Syndrome |
| CN108034672A (en) * | 2017-12-19 | 2018-05-15 | 武汉生物工程学院 | Applications of the nitrate anion transporter gene OsNRT1.9b in rice selection and breeding |
| CN112522259A (en) * | 2020-09-21 | 2021-03-19 | 华南农业大学 | Method for cultivating plant type improved rice material with Oslg1 mutant phenotype through haploid mediation |
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2022
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060003354A1 (en) * | 2004-05-03 | 2006-01-05 | Krantz Ian D | Methods and compositions for the diagnosis of Cornelia de Lange Syndrome |
| CN108034672A (en) * | 2017-12-19 | 2018-05-15 | 武汉生物工程学院 | Applications of the nitrate anion transporter gene OsNRT1.9b in rice selection and breeding |
| CN112522259A (en) * | 2020-09-21 | 2021-03-19 | 华南农业大学 | Method for cultivating plant type improved rice material with Oslg1 mutant phenotype through haploid mediation |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115851760A (en) * | 2022-09-01 | 2023-03-28 | 广东省农业科学院农业质量标准与监测技术研究所 | Rice high-salt-sensitivity mutant gene SS3, mutant SS3 and application |
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| CN114410650B (en) | 2022-10-18 |
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