CN114410503B - Manganese oxidizing bacteria and screening method and application thereof - Google Patents
Manganese oxidizing bacteria and screening method and application thereof Download PDFInfo
- Publication number
- CN114410503B CN114410503B CN202111489885.2A CN202111489885A CN114410503B CN 114410503 B CN114410503 B CN 114410503B CN 202111489885 A CN202111489885 A CN 202111489885A CN 114410503 B CN114410503 B CN 114410503B
- Authority
- CN
- China
- Prior art keywords
- manganese
- oxidizing bacteria
- oxidizing
- manganese oxidizing
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/20—Recycling
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a manganese oxidizing bacterium, a screening method and application thereof, wherein the manganese oxidizing bacterium belongs to Morganella (Morganella Morganii), and is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 22815 in the year 7 and the day 2 of 2021. The manganese oxidizing bacteria screened by the invention is a strain of bacteria which is discovered by Morganella for the first time and has manganese oxidizing capability, and the manganese oxidizing capability of the strain is stronger, the environmental adaptability is good, and the strain has extremely high research value; the method for preparing the biological manganese oxide by oxidizing the manganese oxidizing bacteria has the advantages of simplicity in operation, lower cost and the like, and is beneficial to the application of the method in the field of arsenic detoxification.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a manganese oxidizing bacterium, a screening method and application thereof.
Background
Arsenic (As) is a global pollutant, and after excessive arsenic enters a human body, the oxidation-reduction capability of cells of the human body can be destroyed, normal metabolism of the cells is affected, tissue damage and organism obstruction are caused, and serious people can directly cause toxic death.
Arsenic in the environment As 3+ And As 5+ Both forms exist and As 3+ Is far more toxic than As 5+ Wherein: as As 3+ Toxicity is about As 5+ Toxicity is 60 times. Furthermore, the related studies found that, with As 5+ As compared with 3+ Has higher mobility and lower adsorption rate; the reason for this is that As, when the pH is between 0 and 9.0 3+ The main existing form of (C) is H 3 AsO 3 The surface is uncharged and is not easily adsorbed by charged substances, the mobility is higher, and As 5+ In the presence of H in the form of its predominant form at a pH > 2.0 2 AsO 4- 、HAsO 4 2- And AsO 4 3- Is easy to combine with positively charged substances on the surface, thereby reducing the mobility of the positively charged substances.
As a result, highly toxic and easily migrating As 3+ Oxidation to low toxicity and low mobility As 5+ Is a better choice for reducing the toxicity of the arsenic in the water body.
In the prior art, as 3+ The oxidation technique of (2) includes an oxidizing agent oxidation method, a photocatalytic oxidation method and a biological method.
Among them, biological methods are receiving attention more and more due to the advantages of low cost and no secondary pollution. Manganese oxidizing microorganisms are the wars in microbial oxidation, and biological manganese oxides generated by the oxidation of manganese oxidizing microorganisms have low charge zero point, large surface area, high negative charge, very high activity and very strong oxidation adsorption capacity, are generally coated on the surfaces of other minerals, and have very strong reactivity. As important natural adsorption carriers, redox hosts and chemistryThe catalyst for the reaction, biological manganese oxide plays a decisive role in the aspects of biological effectiveness, physiological toxicity, migration and conversion in the environment, degradation of organic pollutants and the like of inorganic pollutants, and plays an important role in the bio-geochemical circulation of various substances. As can be effectively prepared by high-valence biological manganese oxide 3+ Oxidation to As 5+ Achieves the function of arsenic detoxification, and compared with the function of chemical manganese oxide on arsenic detoxification, the biological manganese oxide has higher oxidation speed and higher efficiency, and manganese oxide reduces Mn formed by trivalent arsenic 2+ Can be continuously reacted by manganese oxidizing bacteria to form biological manganese oxide, thereby achieving the continuous detoxification effect on arsenic.
However, the common manganese oxidizing bacteria have low efficiency on manganese oxidation, and have weak tolerance to arsenic, so that good arsenic detoxification effect cannot be achieved.
Therefore, screening a strain of manganese oxidizing bacteria with high oxidation efficiency on manganese and high tolerance on arsenic is a necessary measure for making up the current deficiency and improving the application of biological manganese oxide in the field of arsenic detoxification.
In view of this, the present invention has been made.
Disclosure of Invention
In view of the above, the invention provides a manganese oxidizing bacterium and a screening method and application thereof.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
the invention provides a manganese oxidizing bacterium which belongs to Morganella genus (Morganella Morganii), is named Morganella sp MnOx-1 and is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 22815 in the 7 th month 2 of 2021.
Specifically, the manganese oxidizing bacteria are obtained by screening and separating sludge collected from the ocean lake wetland in the long-time sandy city of Hunan province by the applicant, and are preserved in the China general microbiological culture Collection center, address: beijing, the Korean yang district, north Chen Xi Lu No. 1, 3, china academy of sciences microbiological study, postal code 100101; the 16S rRNA gene sequence of the recombinant DNA has 99 percent of similarity with the Morganella Morganii S rRNA gene sequence.
The invention also provides a screening method of the manganese oxidizing bacteria, which comprises the following steps:
s1, taking supernatant obtained after standing a sludge sample, and adding the supernatant into a sludge containing MnSO 4 ·H 2 LB liquid medium of O and HEPES buffer;
s2, after shaking culture at constant temperature for 5-9d, checking and preliminary screening the culture medium through the LBB indicator.
Further, in the above technical solution, the screening method of the manganese oxidizing bacteria further includes:
s3, diluting, coating and separating the culture solution which is blue to the LBB indicator in the step S2 and contains MnSO 4 ·H 2 And (3) culturing at constant temperature in a solid culture medium of O and HEPES buffer solution, further checking and re-screening through an LBB indicator, separating and purifying the LBB indicator to be blue, uniformly mixing the obtained strain with glycerol according to the ratio of 1:1, and preserving at-80 ℃.
Specifically, in the above technical solution, in step S1, the LB liquid medium includes the following components:
10g of tryptone, 5g of yeast extract powder, 5g of sodium chloride, 1000ml of distilled water, pH of 7 and MnSO 4 ·H 2 O1 mmol/L, HEPES buffer 20mmol/L.
Specifically, in the above technical solution, in step S2, the preparation method of the LBB indicator is as follows:
0.04g of LBB powder was weighed, dissolved in 0.25ml of 45mmol/L glacial acetic acid aqueous solution, added with deionized water, and kept at a constant volume of 100ml at 4℃in the absence of light.
In one embodiment of the present invention, in step S3, mnSO is contained 4 ·H 2 The preparation method of the solid medium of the O and HEPES buffer solution is as follows:
dissolving tryptone 10g, yeast extract 5g, sodium chloride 5g and agar 15g in distilled water 1000ml, adjusting pH to 7, sterilizing at 121deg.C for 20min, filtering with 0.22 μm filter head, adding 1mol/L MnSO 4 ·H 2 O and 1mol/L HEPES buffer, such that Mn 2+ And HEPES buffer at final concentrations of 1mmol/L and 20mmol/L, respectively,after cooling to 50 ℃, the plates are poured on a sterile operating table, and the solid plates are condensed for later use.
The invention also provides a microbial inoculum containing the manganese oxidizing bacteria.
The invention also provides application of the manganese oxidizing bacteria or the microbial inoculum in preparing biological manganese oxide for oxidizing trivalent arsenic.
The invention also provides application of the manganese oxidizing bacteria or the microbial inoculum in oxidizing trivalent arsenic.
In particular, in the above application of trivalent arsenic oxide, the manganese oxidizing bacteria oxidize Mn by oxidizing Mn 2+ The biological manganese oxide is prepared, and trivalent arsenic is oxidized into pentavalent arsenic through the biological manganese oxide.
Compared with the prior art, the invention has the following advantages:
(1) The manganese oxidizing bacteria screened by the invention is a strain of bacteria which is discovered by Morganella for the first time and has manganese oxidizing capability, and the manganese oxidizing capability of the strain is stronger, the environmental adaptability is good, and the strain has extremely high research value;
(2) The method for preparing the biological manganese oxide by oxidizing the manganese oxidizing bacteria has the advantages of simplicity in operation, lower cost and the like, and is beneficial to the application of the method in the field of arsenic detoxification.
Drawings
FIG. 1 is a colony morphology of manganese oxidizing bacteria in an embodiment of the present invention;
FIG. 2 is a phylogenetic analysis tree of manganese oxidizing bacteria in an embodiment of the present invention;
FIG. 3 is a graph showing manganese oxidation rate of 1mmol/L by manganese oxidizing bacteria in an embodiment of the present invention;
FIG. 4 is a graph showing manganese oxidation rates of manganese oxidizing bacteria at different pH values in an embodiment of the present invention;
FIG. 5 is a graph showing the activity of the manganese oxidizing bacteria at different pH values in the examples of the present invention;
FIG. 6 is a graph showing manganese oxidation rates of manganese oxidizing bacteria at different divalent manganese contents in an embodiment of the present invention;
FIG. 7 is a graph showing the activity of the manganese oxidizing bacteria at different divalent manganese contents in the examples of the present invention;
FIG. 8 is an SEM photograph of biological manganese oxide produced by manganese oxidizing bacteria in the examples of the present invention;
FIG. 9 shows a manganese oxide pair of 100mg/LAs in an embodiment of the invention 3+ Oxidation rate plot.
Detailed Description
The present invention will be described in further detail with reference to the following examples in order to make the objects, technical solutions and advantages of the present invention more apparent.
It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
In the examples, all means used are conventional in the art unless otherwise specified.
The terms "comprising," "including," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, step, method, article, or apparatus.
In addition, the technical features of the embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
Unless otherwise specified, the raw materials used in the examples of the present invention are all commercially available products.
Manganese oxidizing bacteria were deposited at the China general microbiological culture Collection center, with a deposit address: the Beijing city, the Korean district, the North Chen Xiyu No. 1, 3, the institute of microbiology, the academy of China, the postal code 100101, the CGMCC No.22815.
In order to facilitate a further understanding of the present invention, the technical solution of the present invention will now be described in detail with reference to preferred embodiments.
EXAMPLE 1 screening, separation and purification of manganese-oxidizing bacteria
The method comprises the following specific steps:
(1) Preparing a liquid culture medium:
weighing 10g of tryptone, 5g of yeast extract powder and 5g of sodium chloride, adding into 1000ml of distilled water, adjusting pH to 7, subpackaging 100ml into 250ml conical flask, sterilizing at 121deg.C for 20min, filtering with 0.22 μm filter head, adding 1mol/L MnSO4.H 2 O aqueous solution and 1mol/L HEPES buffer, and allowing Mn to react 2+ And HEPES buffer at a final concentration of 1mmol/L and 20mmol/L, respectively.
(2) Preparing a solid culture medium:
weighing 10g of tryptone, 5g of yeast extract powder, 5g of sodium chloride and 15g of agar, adding into 1000ml of distilled water, adjusting pH to 7, sterilizing at 121deg.C for 20min, filtering with 0.22 μm filter head, adding 1mol/L MnSO4.H 2 O aqueous solution and 1mol/L HEPES buffer, such that Mn 2+ And HEPES buffer solution with final concentration of 1mmol/L and 20mmol/L respectively, pouring the culture medium to a flat plate on a sterile operating table when the culture medium is cooled to 50 ℃, and condensing the solid flat plate for later use.
(3) Screening and separating manganese oxidizing bacteria:
collecting sludge from the ocean lake wetland in the Yangsha city of Hunan province, standing, taking supernatant, adding the supernatant into the liquid culture medium in the step (1) according to the inoculation amount of 1%, carrying out shake culture on the mixture for 7 days on a constant-temperature shaking table at 30 ℃ and 150rpm, qualitatively detecting whether manganese oxide is generated by using an LBB indicator, and carrying out primary screening on the culture solution with the LBB indicator changing from colorless to blue.
The preparation method of the LBB indicator comprises the following steps: 0.04g of LBB powder was weighed, dissolved in 0.25ml of glacial acetic acid aqueous solution (45 mmol/L), deionized water was added and the volume was set to 100ml, and stored at 4℃in the dark.
(4) Purification of manganese oxidizing bacteria
Diluting, coating and separating the culture solution which shows blue color to the LBB indicator in the step (3) in the solid culture medium in the step (2), placing in a baking oven at the constant temperature of 30 ℃ for culturing, observing the colony morphology, carrying out a color development experiment by adopting the LBB indicator, further streaking, separating and purifying the blue colony, uniformly mixing the purified strain with glycerol according to the ratio of 1:1, and preserving in a refrigerator at the temperature of minus 80 ℃.
EXAMPLE 2 morphological characterization and molecular characterization of manganese oxidizing bacteria
Morphological characteristics:
as shown in FIG. 1, the strain is in a shape of a rod as a whole, the colony is in a shape of a circle, the center is in an opaque milky color, the surface is convex and moist, and the edge is in a regular brown color in combination with the scanning electron microscope result.
Molecular identification and phylogenetic tree alignment:
DNA extraction is carried out on the separated and purified manganese oxide bacteria, related extraction operation is completed by adopting a bacterial DNA extraction kit produced by the Magen company, and then PCR amplification is carried out, wherein the primers are bacterial 16S rDNA universal primers (1492R- 'GGTTACCTTGTTACGA CTT',27F- 'AGAGTTTGATCMTGGCTCAG'). Sequencing of the PCR products was performed by Shanghai Bioengineering Co. The 16S rDNA results from the sequencing (shown as Seq ID No: 1) were compared for similarity to existing 16S rDNA nucleic acid sequences by the Blast program of NCBI (national center for Biotechnology information). Analysis and drawing of phylogenetic tree were performed by mega4.0 software, and the phylogenetic tree is shown in fig. 2.
Sequence result analysis shows that the manganese oxidizing bacteria have the highest similarity with Morganella Morganii, are identified as belonging to Morganella, and are named as MnOx-1.
EXAMPLE 3 determination of manganese oxidizing Capacity of manganese oxidizing bacteria MnOx-1
(1) A 250ml conical flask was selected and 100m1 of manganese-containing liquid medium was prepared in the manner described in step (1) of example 1, namely: weighing 10g of tryptone, 5g of yeast extract powder and 5g of sodium chloride, adding into 1000ml of distilled water, adjusting pH to 7, subpackaging 100ml into 250ml conical flask, sterilizing at 121deg.C for 20min, filtering with 0.22 μm filter head, adding 1moL/L MnSO4.H 2 O aqueous solution and 1moL/L HEPES buffer, and allowing Mn to act 2+ And HEPES buffer at a final concentration of 1mmol/L and 20mmol/L, respectively.
(2) Adding manganese oxidizing bacteria into the manganese-containing liquid culture medium in the step (1) according to the inoculation amount of 1%, placing each conical flask into a constant-temperature shaking table at 30 ℃ and 180rpm for shaking culture, and carrying out 3 parallel and 1 blank control on each group; samples were taken every 24 hours for a time less than 10ml centrifuge tube, using centrifuge 8000Centrifuging at rpm for 3min, collecting supernatant, and measuring residual Mn of the culture solution by inductively coupled plasma emission spectrometry (ICP-OES) 2+ Concentration, sampling was continued for 7 days until the end of the experiment.
(3) The method for calculating the manganese oxidation rate comprises the following steps:
manganese oxidation rate = (initial manganese content-remaining manganese content)/initial manganese content 100%.
As shown in FIG. 3, it is apparent from the analysis of the results of FIG. 3 that the manganese-oxidizing bacteria pair Mn 2+ The oxidation speed is high, mn is high in 1-2 days 2+ Is rapidly oxidized, mn at 2-7 days 2+ The oxidation gradually tends to be stable, the maximum manganese oxidation rate reaches 88.17 percent, and the manganese oxidation capability is extremely strong.
Example 4 determination of manganese Oxidation Capacity at different pH and divalent manganese content
(1) Determination of manganese Oxidation Capacity under different pH conditions
Preparing a manganese-containing liquid culture medium in the manner of step (1) in example 3, adjusting the pH in the manganese-containing liquid culture medium to ensure that the pH gradients are 5, 6, 7, 8 and 9 respectively, adding 100ml of the manganese-containing liquid culture medium into a 250ml conical flask, inoculating bacterial solutions into manganese-containing liquid culture media with different pH values according to the inoculum size of 1 percent, and performing shaking culture at 180rpm and 30 ℃ at constant temperature, wherein each group is 3 in parallel and 1 blank control.
Sampling from different culture mediums according to a certain time, sampling for 7 days, measuring Mn residual quantity in a centrifuge tube after filtering by an atomic absorption spectrometry, measuring an OD600 value by an ultraviolet spectrophotometer, and respectively determining manganese oxidation rate and manganese oxidizing bacteria activity under different pH values.
As shown in FIG. 4, the manganese oxidizing bacteria MnOx-1 has better manganese oxidation rate at pH 5-9, and the manganese oxidation rate reaches about 80% at about 72 h; wherein, the manganese oxidation rate is slightly lower when the pH value is=5, the manganese oxidation rate removal effect is similar when the pH values are=6, 7, 8 and 9, and the manganese oxide bacteria manganese oxide on the surface has better oxidation efficiency in neutral partial alkali environment.
As shown in FIG. 5, the activity of the manganese oxidizing bacteria is similar at the pH of 5-9, which shows that the bacteria has wider adaptability and can grow better in acidic to alkaline environments.
(2) Determination of manganese Oxidation Capacity at different divalent manganese content
Preparing a manganese-containing liquid culture medium in the manner of step (1) in example 3, and adjusting Mn in the manganese-containing liquid culture medium 2 The +content is 1, 5, 10, 15, 20mmol/L respectively, add 100ml manganese-containing liquid culture medium into 250ml conical flask, inoculate the bacterial liquid into liquid culture medium with different bivalent manganese content according to 1% inoculum size, shake culture at 180rpm and 30 ℃,3 parallel, 1 blank control each.
Sampling from different culture mediums according to a certain time, sampling for 7 days, measuring Mn residual quantity in a centrifuge tube after filtering by an atomic absorption spectrometry, measuring an OD600 value by an ultraviolet spectrophotometer, and respectively determining manganese oxidation rate and manganese oxidizing bacteria activity under different divalent manganese contents.
As shown in FIG. 6, the manganese oxidizing bacteria are present in Mn 2+ Manganese oxidation rates at levels of 1, 5, 10, 15, 20mmo/L vary greatly with Mn 2+ The content is increased, when the manganese oxidation rate reaches the maximum at 5mmol/L, mn is continuously added 2+ The manganese oxidation rate gradually decreases, and at 20mmol/L, the manganese oxidation rate is the lowest, which is only about 20%.
As shown in FIG. 7, the change in the content of divalent Mn has a large effect on the activity of the manganese oxidizing bacteria, and a certain amount of Mn is added 2+ Has a certain promoting effect on the growth of manganese oxidizing bacteria, but when Mn 2+ When the content is too high, the growth of manganese oxidizing bacteria is obviously inhibited, such as Mn 2+ At a content of 20mmol/L, the growth of the manganese oxide bacteria is obviously slower.
Example 5 biological manganese oxide formation and production of As 3+ Oxidation experiments of (2)
(1) Production of biological manganese oxide
100ml of manganese-containing liquid medium was prepared in the manner described in step (1) of example 1, namely: weighing 10g of tryptone, 5g of yeast extract powder and 5g of sodium chloride, adding into 1000ml of distilled water, adjusting pH to 7, sterilizing at 121deg.C for 20min, filtering with 0.22 μm filter head, adding 1mol/L MnSO4.H 2 O aqueous solution and 1mol/L HEPES buffer, and allowing Mn to react 2+ And HEPES buffer at final concentrations of 1mmol/L and 20mmol/L, respectively; according to 1%Adding manganese oxidizing bacteria into manganese-containing liquid culture medium, shaking and culturing at 30deg.C and 180rpm in a constant temperature shaking table for 7 days, centrifuging at 8000rpm in a centrifuge for 3min, discarding supernatant, washing precipitate with deionized water for 3 times, and obtaining precipitate as biological manganese oxide.
(2) Scanning Electron Microscope (SEM) analysis of biological manganese oxide
The biological oxide is formed by adding manganese oxidizing bacteria into a liquid culture medium containing divalent manganese, a Scanning Electron Microscope (SEM) image is shown in figure 8, and under the magnification of 10000 times, the scanning electron microscope shows that the surface of the biological manganese oxide is irregular, takes a strip shape or smooth spar shape, and a large number of strip-shaped manganese oxidizing bacteria are filled around, so that the biological manganese oxide is a state of being mixed with the manganese oxidizing bacteria.
(3) Biological manganese oxide pair As 3+ Oxidation experiments of (2)
100ml of manganese-containing liquid medium was prepared in the manner described in step (1) of example 1, namely: weighing 10g of tryptone, 5g of yeast extract powder and 5g of sodium chloride, adding into 1000m1 of distilled water, regulating pH to 7, sterilizing at 121 ℃ for 20min, wherein 1mol/L MnSO4.H is added by using a 0.22 μm filter head for filtration 2 O aqueous solution, 1mol/L HEPES buffer and As 3+ Solution and make Mn 2+ HEPES buffer and As 3+ The final concentration of (2) is 1mmol/L, 20mmol/L and 100mg/L, respectively; adding manganese oxidizing bacteria into the above Mn-containing strain at 1% inoculation amount 2+ HEPES buffer and As 3+ Is cultured by shaking in a shaking table at a constant temperature of 30℃and 180 rpm.
Sampling is carried out every 24 hours, 1 microliter of culture solution is sucked and diluted to a 10ml centrifuge tube, then 1ml in the 10ml centrifuge tube after dilution is sucked and further diluted to a new 10ml centrifuge tube, the total dilution is 1000 times, and the sampling lasts for 7 days.
As in the reaction process by adopting an inductively coupled plasma mass spectrometer (ICP-MS) 3+ /As 5+ Is measured.
As can be seen in FIG. 9, the biological manganese oxide pair As produced by the strain of the present invention 3+ The oxidation is faster, and 50.98 percent of As can be oxidized after 3 hours of initial reaction 3+ And over time，As 3+ Gradually oxidized further and after 7 days, for As 3+ Finally, the oxidation effect of about 74% is achieved. The manganese oxidizing bacteria produced by the strain of the present invention showed a protective effect against As 3+ Good oxidation effect, can be used for preparing As 3+ Oxidation to less toxic As 5+ Is beneficial to being applied in the field of arsenic detoxification.
In conclusion, the novel manganese oxidizing bacteria are screened out, are bacteria with manganese oxidizing ability which are first discovered in Morganella, have strong manganese oxidizing ability, are good in environmental adaptability and have extremely high research value; as can be obtained from the biological manganese oxide produced by the bacterium 3+ Oxidation to As 5+ The method is beneficial to being applied to the arsenic detoxification field, and simultaneously provides a theoretical basis for the application potential of the biological manganese oxide possibly existing.
It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (5)
1. A manganese oxidizing bacterium is characterized in that,
belongs to Morganella (Morganella Morganii), is named MnOx-1 and is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 22815 in the 7 th month of 2021.
2. A microbial inoculum comprising the manganese oxidizing bacteria according to claim 1.
3. Use of the manganese oxidizing bacteria of claim 1 or the microbial inoculum of claim 2 for the preparation of a biological manganese oxide for oxidizing trivalent arsenic.
4. Use of the manganese oxidizing bacteria of claim 1 or the microbial inoculum of claim 2 for oxidizing trivalent arsenic.
5. The use according to claim 4, wherein,
the manganese oxidizing bacteria oxidize Mn by oxidizing Mn 2+ The biological manganese oxide is prepared, and trivalent arsenic is oxidized into pentavalent arsenic through the biological manganese oxide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111489885.2A CN114410503B (en) | 2021-12-08 | 2021-12-08 | Manganese oxidizing bacteria and screening method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111489885.2A CN114410503B (en) | 2021-12-08 | 2021-12-08 | Manganese oxidizing bacteria and screening method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114410503A CN114410503A (en) | 2022-04-29 |
| CN114410503B true CN114410503B (en) | 2023-10-03 |
Family
ID=81264699
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111489885.2A Active CN114410503B (en) | 2021-12-08 | 2021-12-08 | Manganese oxidizing bacteria and screening method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114410503B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993844A (en) * | 2010-09-08 | 2011-03-30 | 湖南中烟工业有限责任公司 | Microorganism for degrading nicotine and application thereof |
| CN102382785A (en) * | 2011-10-19 | 2012-03-21 | 浙江工业大学 | Morganella morganii and application thereof in preparation of (S)-2-carboxyethyl-3-cyano-5-methylhexanoic acid |
| CN104619652A (en) * | 2012-06-15 | 2015-05-13 | 微视生物技术有限公司 | Novel biocatalyst compositions and processes for use |
| CN105238716A (en) * | 2015-10-17 | 2016-01-13 | 厦门大学 | Morganella sp. and application thereof to microbial fuel cells |
| CN108384731A (en) * | 2018-02-05 | 2018-08-10 | 华中农业大学 | A kind of manganese oxidizing bacteria and its screening technique and application |
| CN111378597A (en) * | 2020-01-17 | 2020-07-07 | 合肥工业大学 | Manganese oxidizing bacterium capable of being used for efficient demanganization and application thereof |
| CN112094766A (en) * | 2020-06-09 | 2020-12-18 | 浙江万里学院 | Manganese oxidizing bacteria and application thereof |
| CN113727722A (en) * | 2019-02-22 | 2021-11-30 | 伊夫罗生物科学公司 | Bacterial membrane preparation |
-
2021
- 2021-12-08 CN CN202111489885.2A patent/CN114410503B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993844A (en) * | 2010-09-08 | 2011-03-30 | 湖南中烟工业有限责任公司 | Microorganism for degrading nicotine and application thereof |
| CN102382785A (en) * | 2011-10-19 | 2012-03-21 | 浙江工业大学 | Morganella morganii and application thereof in preparation of (S)-2-carboxyethyl-3-cyano-5-methylhexanoic acid |
| CN104619652A (en) * | 2012-06-15 | 2015-05-13 | 微视生物技术有限公司 | Novel biocatalyst compositions and processes for use |
| CN105238716A (en) * | 2015-10-17 | 2016-01-13 | 厦门大学 | Morganella sp. and application thereof to microbial fuel cells |
| CN108384731A (en) * | 2018-02-05 | 2018-08-10 | 华中农业大学 | A kind of manganese oxidizing bacteria and its screening technique and application |
| CN113727722A (en) * | 2019-02-22 | 2021-11-30 | 伊夫罗生物科学公司 | Bacterial membrane preparation |
| CN111378597A (en) * | 2020-01-17 | 2020-07-07 | 合肥工业大学 | Manganese oxidizing bacterium capable of being used for efficient demanganization and application thereof |
| CN112094766A (en) * | 2020-06-09 | 2020-12-18 | 浙江万里学院 | Manganese oxidizing bacteria and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| An implication of biotransformation in detoxification of mercury contamination by Morganella sp. strain IITISM23;Singh, Shalini等;ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH;第第28卷卷(第第27期期);35661-35677 * |
| 摩氏摩根菌研究进展;廖中华 等;中国动物传染病学报;1-8 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114410503A (en) | 2022-04-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108865908B (en) | High-antimony-resistance rhodotorula mucilaginosa DJHN070401, and separation and screening method and application thereof | |
| CN110938569B (en) | Microbial agent for hexavalent chromium pollution treatment and hexavalent chromium pollution treatment method | |
| WO2021114784A1 (en) | Strain capable of tolerating high concentration of chromium, and microbial agent used for hexavalent chromium pollution control | |
| CN112725230B (en) | Selenium-resistant strain Enterobacter ludwigii GX-C3 and application thereof | |
| CN112538449B (en) | Alcaligenes faecalis DY-8 and application thereof in removal of heavy metal cadmium | |
| CN116790439A (en) | Bacillus bailii and application thereof in degradation of COD (chemical oxygen demand) of wastewater | |
| CN110846254A (en) | Compound microbial agent for denitrification and preparation method and application thereof | |
| CN114410503B (en) | Manganese oxidizing bacteria and screening method and application thereof | |
| CN110734886B (en) | Bacterial strain capable of tolerating high-concentration chromium and application thereof | |
| CN114854630B (en) | Selenium-resistant bacillus and breeding method and application thereof | |
| CN112574927B (en) | Selenium-resistant strain GX-B4 and application thereof | |
| CN112553130B (en) | Selenium-resistant strain GX-D6 and application thereof | |
| CN114703104A (en) | Bacterial strain with iron reduction capacity and electrochemical activity and application thereof | |
| CN111378597B (en) | Manganese oxidizing bacterium capable of being used for demanganization and application thereof | |
| CN111378596B (en) | Acid-resistant and facultative anaerobic manganese oxidizing bacterium and application thereof | |
| CN114107101A (en) | Cadmium and chromium-fixed cupronickel bacterium and application thereof in co-remediation of cadmium and chromium composite polluted soil | |
| CN112831431A (en) | Selenium-resistant strain Comamonas testosteroni GX-A1 and application thereof | |
| CN114634888B (en) | Thallium oxidizing bacteria, microbial agent and application thereof | |
| CN114149940B (en) | Achromobacter, microbial inoculum containing same and application thereof | |
| CN111662847A (en) | Enterobacter antimonoroxide and application thereof | |
| CN114410552B (en) | Pseudomonas gainsteri for oxidizing sulfur and application of pseudomonas gainsteri in sulfur reduction in black and odorous water body air | |
| CN114686404B (en) | Strain with iron reduction capability and power generation capability and application thereof | |
| CN114058517B (en) | Fungus and algae mixture, fungus and algae symbiotic system, construction method and application thereof | |
| CN111154681B (en) | Efficient anaerobic antimony reduction strain and application thereof | |
| CN117535162A (en) | Zygosaccharomyces bailii strain Y19 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |