CN114405564B - Microfluidic biochip for detecting nucleic acid and production process - Google Patents
Microfluidic biochip for detecting nucleic acid and production process Download PDFInfo
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- CN114405564B CN114405564B CN202210065415.1A CN202210065415A CN114405564B CN 114405564 B CN114405564 B CN 114405564B CN 202210065415 A CN202210065415 A CN 202210065415A CN 114405564 B CN114405564 B CN 114405564B
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- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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Abstract
The invention belongs to the field of microfluidic biochips, in particular to a microfluidic biochip for detecting nucleic acid and a production process thereof, wherein a cutter, a blanking pipe, a pressing plate, a round buckling rod, a fixing buckle, a push rod and a blanking slide plate are arranged; the arm drives the clamp plate and is close to the chip board of base top surface, the bottom of circle knot pole inserts the fixed orifices on the chip board and fixes the chip board, circle knot pole slides to the mounting groove, the cooperation is fixed detain, it is fixed with the circle knot pole, the cutter cuts the chip board, biochip falls into unloading intraductal packing, the arm drives the chip board that the cutting was accomplished and removes unloading slide department, the lateral wall of unloading slide is collided to the push rod, release circle knot pole, circle knot pole slides downwards under the elasticity effect of spring No. one for circle knot pole shakes, shake the chip board and fall the unloading slide with the chip board, the work of cutting and the unloading of waste material of snatching of chip board has been accomplished, biochip's dispersion packing work, the staff of being convenient for selects the biochip that has the flaw.
Description
Technical Field
The invention belongs to the field of microfluidic biochips, and in particular relates to a microfluidic biochip for detecting nucleic acid and a production process thereof.
Background
The microfluidic biochip is a whole process of integrating basic operation elements of biological, chemical and medical analysis on the chip and automatically completing the whole process; the microfluidic chip has the characteristics of controllable liquid flow, extremely small consumption of samples and reagents, ten times or hundreds times of analysis speed improvement and the like, can analyze hundreds of samples simultaneously in extremely short time, can realize the whole pretreatment and analysis processes of the samples on line, and plays an irreplaceable role in the large-scale nucleic acid detection process.
One chinese patent with publication number CN106190829a discloses a microfluidic biochip for high-precision arrangement and detection of cells, comprising a cover sheet and a slide, wherein a liquid feeding region, a cell arrangement microchannel, an incident optical fiber, a guiding-out optical fiber or a micro-photodetector, a fluid expansion region, a cell detection region and a liquid drainage region are provided on the cover sheet; the cells after fluorescent staining enter the micro-channel from the liquid adding area, pass through the cell arrangement micro-channel, are arranged on a straight line with high precision, are decelerated by the fluid expansion area, and sequentially pass through the cell detection area; the laser is led into a cell detection area through an incident optical fiber, cells to be detected are sequentially irradiated, fluorescence is excited, the excited fluorescence is led out through a leading-out optical fiber or is subjected to photoelectric conversion and led out through a micro photoelectric detector, signal analysis is carried out, accurate detection of the cells is realized, and the detected cell sample liquid flows out through a liquid discharge area for inactivation and other treatments; the invention can realize high-precision arrangement and accurate and portable detection of cells, and has important application potential in the fields of biomedicine, food science and the like.
When the existing biochip is produced, a plurality of chips are mixed and packed into the same packaging bag, and when a numbered biochip is defective in production, the numbered biochip in all the packaging bags needs to be picked out, so that the working difficulty and the workload are greatly increased, and even the chips of the whole packaging bag are scrapped.
Therefore, the invention provides a microfluidic biochip for nucleic acid detection and a production process.
Disclosure of Invention
In order to overcome the deficiencies of the prior art, at least one technical problem presented in the background art is solved.
The technical scheme adopted for solving the technical problems is as follows: the invention relates to a microfluidic biochip for detecting nucleic acid, which comprises a chip body and a sealing cover; the chip comprises a chip body, and is characterized in that a hole channel is formed in the chip body, a sample adding port is formed in one end of the chip body, the sample adding port is communicated with the hole channel, a nano fluorescent probe is arranged at one end, close to the hole channel, of the sample adding port, a sealing cover is fixedly connected to the side surface of one end, close to the sample adding port, of the chip body, and the outer wall of the sealing cover is in threaded fit with the inner wall of the sample adding port; through with sealed lid and the application of sample mouth screw-thread fit of chip body, improved the sealed effect to the chip body, reduced the chip body of the liquid department of splashing of adding, improved the security that detects.
A production process of a microfluidic biochip for nucleic acid detection, the production process being applicable to the microfluidic biochip, and the production process comprising the steps of:
s1: adding the adhesive A and the adhesive B into a vacuum defoaming stirrer according to the proportion of 10:1, and uniformly stirring and mixing in vacuum;
s2: adding the stirred and mixed glue into a forming die, performing injection molding, and cooling and solidifying the chip board;
s3: moving the chip board onto a conveyor belt, punching the chip, and then cleaning, sterilizing and drying the chip;
s4: the chip board is carried to the unloader by the conveyer belt, and the arm snatchs the chip board that will convey to the base top, and the chip board is sent the top of cutting board, and the biochip on the chip board is cut off to the cutter, and biochip is separately wheredown by a plurality of unloading pipes that correspond, carries out the dispersion packing with the chip.
Preferably, the blanking device in S4 includes a base, a mechanical arm, a grabbing structure, a cutting plate, a cutter and a blanking pipe; the chip board is placed on the top surface conveyor belt of the base, a mechanical arm is arranged on one side of the base, a grabbing structure is arranged at one end, far away from the base, of the mechanical arm, a cutting board is fixedly connected to one side, far away from the mechanical arm, of the base, a plurality of blanking pipes are fixedly connected to the bottom surface of the cutting board, the blanking pipes penetrate through the cutting board, packaging bags are sleeved on the outer ring of the blanking pipes, a cutter is fixedly connected to the top surface of the cutting board, and an inner ring of the cutter is sleeved on the inner ring of the blanking pipes; during operation, the mechanical arm drives the grabbing structure to move, the chip plate conveyed to the top of the base is grabbed, the chip plate is conveyed to the top of the cutting plate by the mechanical arm, the biochip on the chip plate is cut off by the cooperation of the cutter, and the biochip is separated and dropped by the corresponding plurality of blanking pipes, so that the independent packaging of the biochip is completed, and the biochip with flaws is conveniently selected by workers.
Preferably, the grabbing structure comprises a pressing plate and a round buckling rod, the bottom end of the mechanical arm is fixedly connected with the pressing plate, a plurality of mounting grooves are formed in the bottom surface of the pressing plate, the round buckling rod is slidably mounted in the mounting grooves, a conical frustum is arranged at the top of the round buckling rod, a fixing hole matched with the round buckling rod is formed in the middle of the chip board, a spring is mounted on the top surface of the round buckling rod, a reset groove is formed in one side of the mounting groove, a fixing buckle is slidably arranged in the reset groove, a second spring is mounted on the fixing buckle, a push rod is slidably mounted in the pressing plate, a sliding groove matched with the push rod is formed in the pressing plate, the push rod is fixedly connected with the fixing buckle, a blanking sliding plate is fixedly connected on one side of the cutting board, and the push rod is slidably matched with the blanking sliding plate; when the mechanical arm works, the mechanical arm drives the pressing plate to move downwards, so that the pressing plate is close to the chip plate on the top surface of the base, the bottom of the round buckling rod is inserted into the fixing hole on the chip plate to fix the chip plate, after the bottom end of the round buckling rod contacts the top surface of the base, the round buckling rod slides into the mounting groove, the first spring is compressed, the top cone frustum of the round buckling rod extrudes the fixing buckle, the fixing buckle slides into the reset groove, the second spring is compressed, after the top cone frustum of the round buckling rod slides over the fixing buckle, the second spring resets to push the fixing buckle to clamp the round buckling rod, the round buckling rod is fixed, the mechanical arm drives the chip plate to move to the cutting plate, promote clamp plate downwardly moving, the cooperation cutter cuts the chip board, after the cutting is accomplished, the arm drives the chip board and removes unloading slide department for the push rod bumps the lateral wall of unloading slide, make the push rod slide along the spout, drive fixed knot and slide to the inslot that resets, release the circle knot pole, the circle knot pole slides down under the elasticity effect of spring No. one, the diapire of circle knot pole striking mounting groove, make the circle knot pole shake, shake the chip board and fall the unloading slide, thereby accomplished the clamp to the chip board get with the unloading work of waste material, reduced the clamp to the chip board get damage degree.
Preferably, a straight notch is formed in one surface of the pressing plate, which is close to the reset groove, the straight notch is communicated with the reset groove, a sleeve seat is slidably arranged in the reset groove, a fixing buckle is slidably arranged in the sleeve seat, a second spring is fixedly connected between the fixing buckle and the inner wall of the sleeve seat, a long screw is arranged on the sleeve seat, which is close to the straight notch, and the long screw penetrates through the straight notch in a sliding manner; when the circular buckle rod is in operation, the long screw rod is loosened, the sleeve seat is released, the long screw rod is pushed to move along the straight slot, the sleeve seat is driven to move in the reset slot, the position of the circular buckle rod in the reset slot is adjusted and fixed, the sliding distance of the circular buckle rod is controlled, and then the depth of the circular buckle rod inserted into the fixed hole is adjusted by workers conveniently.
Preferably, the outer ring of the bottom of the round buckling rod is provided with a plurality of rubber strips, one side of the rubber strips, which is far away from the round buckling rod, is provided with saw teeth, and the outer walls of the rubber strips are in sliding fit with the inner walls of the fixing holes; during operation, through the rubber strip piece with the sawtooth of seting up, improved the friction force of circle knot pole and fixed orifices, improved the circle knot pole and snatched the firm degree of chip board.
Preferably, the middle part of the top surface of the mounting groove is fixedly connected with a guide rod, a round hole matched with the guide rod is formed in the middle part of the round buckling rod, the outer ring of the guide rod is sleeved on the inner ring of a first spring, a plurality of expansion grooves are formed in the outer ring of the bottom of the round buckling rod, rubber strip blocks are slidably mounted in the expansion grooves, a sleeve is fixedly connected to the bottom of the round hole, a compression rod is slidably mounted at the top of the sleeve, an elliptical marble is fixedly connected between the bottom surface of the compression rod and the bottom surface of the sleeve, an arc push plate is slidably mounted on the outer ring of the sleeve, a plurality of connecting rods are fixedly connected to one side, close to the inner wall of the sleeve, of the arc push plate, one end, far away from the sleeve, of each connecting rod is fixedly connected with the corresponding rubber strip block, a third spring is sleeved on the outer ring of each connecting rod, and two ends of the third spring are fixedly connected with the rubber strip blocks and the outer wall of the sleeve respectively; when the device works, after the bottom end of the round buckling rod contacts the top surface of the base, the round buckling rod slides into the mounting groove, the guide rod slides into the round hole, the bottom end of the guide rod pushes the compression rod to slide into the sleeve, the oval marble is extruded, the height of the oval marble is reduced, the diameter of the oval marble expands, the arc-shaped push plate is pushed to slide into the inner wall of the sleeve, the connecting rod is pushed to push the rubber strip to slide out of the expansion groove, and a plurality of rubber strips are unfolded in the fixing hole, so that the stability of the round buckling rod grabbing chip board is further improved.
Preferably, a rubber plate is slidably arranged in the groove on the top surface of the cutting plate, the circular groove of the rubber plate is sleeved on the outer ring of the cutter, the bottom surface of the rubber plate is fixedly connected with a plurality of fourth springs, and the bottom ends of the fourth springs are fixedly connected with the top surface of the cutting plate; during operation, the arm drives clamp plate and chip board and removes in the top surface recess of cutting board, and the clamp plate promotes the chip board and moves down, cooperates the rubber slab to hold the chip board fixedly, has improved the stability when the cutter cuts the chip board.
Preferably, a plurality of air cavities are formed in the rubber plate, one end of each air cavity is communicated with a round groove of the rubber plate, and a plurality of vertical grooves are formed in the cutter; when the device works, the pressing plate extrudes the rubber plate, so that the rubber plate slides downwards, the rubber plate is compressed, gas in the air cavity is pressed into the circular groove of the rubber plate, the cut biochip is blown, the smoothness of the biochip falling is improved, and the probability of the biochip blocking the blanking pipe is reduced.
The beneficial effects of the invention are as follows:
1. the invention relates to a microfluidic biochip for detecting nucleic acid and a production process thereof, wherein a cutter, a blanking pipe, a pressing plate, a round buckling rod, a fixed buckle, a push rod and a blanking slide plate are arranged; the arm drives the clamp plate and is close to the chip board of base top surface, the bottom of circle knot pole inserts the fixed orifices on the chip board and fixes the chip board, circle knot pole slides to the mounting groove in, the cooperation is fixed detains the pole is fixed with the circle, the cutter cuts the chip board, biochip falls into unloading intraductal packing, the arm drives the chip board that the cutting was accomplished and removes unloading slide department, the lateral wall of unloading slide is collided to the push rod, release circle knot pole, circle knot pole slides downwards under the elasticity effect of spring No. one for circle knot pole shakes, shake the chip board and fall the unloading slide with the chip board, accomplished the work of cutting and the unloading of waste material of snatching of chip board, the staff of being convenient for selects the biochip that has the flaw.
2. The invention relates to a microfluidic biochip for detecting nucleic acid and a production process thereof, wherein a guide rod, a rubber strip block, a compression bar, an arc push plate, a connecting rod and an elliptical marble are arranged; the guide arm slides into in the round hole, and the bottom of guide arm promotes the depression bar and slides to telescopic inside, extrudees oval marble for oval marble height reduces, and the diameter expands, promotes the arc push pedal and slides to telescopic inner wall, promotes the connecting rod and promotes rubber strip piece roll-off expansion tank, makes a plurality of rubber strip pieces expand in the fixed orifices, has further improved the circle knot pole and snatches the firm degree of chip board.
Drawings
The invention is further described below with reference to the accompanying drawings.
FIG. 1 is a schematic diagram of the structure of a biochip of the invention;
FIG. 2 is a perspective view of a first embodiment of the present invention;
FIG. 3 is a schematic diagram of a blanking apparatus according to a first embodiment of the present invention;
FIG. 4 is a partial cross-sectional view taken at A-A of FIG. 3;
FIG. 5 is an enlarged view of a portion of FIG. 3 at B;
FIG. 6 is a partial cross-sectional view of a rubber sheet according to the first embodiment of the present invention;
FIG. 7 is a partial cross-sectional view of a rubber sheet according to a second embodiment of the present invention;
FIG. 8 is a production flow diagram of the present invention;
in the figure: 1. a base; 2. a mechanical arm; 3. cutting the plate; 4. a cutter; 5. discharging pipes; 6. a chip board; 7. a pressing plate; 8. a mounting groove; 9. a round buckling rod; 10. a fixing hole; 11. a first spring; 12. a reset groove; 13. a fixing buckle; 14. a second spring; 15. a chute; 16. a push rod; 17. a blanking slide plate; 18. a straight slot; 19. a sleeve seat; 20. a long screw; 21. rubber strips; 22. a round hole; 23. a guide rod; 24. an expansion slot; 25. a sleeve; 26. a compression bar; 27. an elliptical pachinko ball; 28. an arc push plate; 29. a connecting rod; 30. a third spring; 31. a rubber plate; 32. a spring IV; 33. an air cavity; 34. a knife stone; 35. a chip body; 36. sealing cover.
Detailed Description
The invention is further described in connection with the following detailed description in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
Example 1
As shown in fig. 1, a microfluidic biochip for nucleic acid detection according to an embodiment of the present invention includes a chip body 35 and a sealing cap 36; the inside of the chip body 35 is provided with a hole channel, one end of the chip body 35 is provided with a sample adding port, the sample adding port is communicated with the hole channel, one end of the sample adding port, which is close to the hole channel, is provided with a nano fluorescent probe, one side surface of one end of the chip body 35, which is close to the sample adding port, is fixedly connected with a sealing cover 36, and the outer wall of the sealing cover 36 is in threaded fit with the inner wall of the sample adding port; through the screw thread fit of the sealing cover 36 and the sample adding port of the chip body 35, the sealing effect on the chip body 35 is improved, the chip body 35 at the splashing position of the added liquid is reduced, and the detection safety is improved.
As shown in fig. 8, a production process of a microfluidic biochip for nucleic acid detection, which is applicable to the above microfluidic biochip, comprises the steps of:
s1: adding the adhesive A and the adhesive B into a vacuum defoaming stirrer according to the proportion of 10:1, and uniformly stirring and mixing in vacuum;
s2: adding the stirred and mixed glue into a forming die, performing injection molding, and cooling and solidifying the chip board 6;
s3: the chip board 6 is moved to a conveyor belt, the chip is perforated, and then the chip is cleaned, disinfected and dried;
s4: the chip board 6 is carried to the blanking device by the conveyor belt, the chip board 6 conveyed to the top of the base 1 is grabbed by the mechanical arm 2, the chip board 6 is conveyed to the top of the cutting board 3, the biological chips on the chip board 6 are cut off by the cutter 4, and the biological chips are separated and dropped by the corresponding blanking pipes 5 to carry out dispersed packaging on the chips.
As shown in fig. 2, the blanking device in S4 includes a base 1, a mechanical arm 2, a grabbing structure, a cutting board 3, a cutter 4 and a blanking pipe 5; a chip plate 6 is placed on a top surface conveyor belt of the base 1, a mechanical arm 2 is arranged on one side of the base 1, a grabbing structure is arranged at one end, away from the base 1, of the mechanical arm 2, a cutting plate 3 is fixedly connected to one side, away from the mechanical arm 2, of the base 1, a plurality of blanking pipes 5 are fixedly connected to the bottom surface of the cutting plate 3, the blanking pipes 5 penetrate through the cutting plate 3, packaging bags are sleeved on the outer ring of the blanking pipes 5, a cutter 4 is fixedly connected to the top surface of the cutting plate 3, and the inner ring of the cutter 4 is sleeved on the inner ring of the blanking pipes 5; during operation, the mechanical arm 2 drives the grabbing structure to move, the chip plate 6 conveyed to the top of the base 1 is grabbed, the chip plate 6 is conveyed to the top of the cutting plate 3 by the mechanical arm 2, the biochips on the chip plate 6 are cut off by the cooperation of the cutter 4, and the biochips are separated and dropped by the corresponding blanking pipes 5, so that the individual packaging of the biochips is completed, and workers can conveniently select the biochips with flaws.
As shown in fig. 2 to 4, the grabbing structure comprises a pressing plate 7 and a round buckling rod 9, the bottom end of the mechanical arm 2 is fixedly connected with the pressing plate 7, a plurality of installation grooves 8 are formed in the bottom surface of the pressing plate 7, the round buckling rod 9 is slidably installed in the installation grooves 8, a conical frustum is arranged at the top of the round buckling rod 9, a fixing hole 10 matched with the round buckling rod 9 is formed in the middle of the chip board 6, a first spring 11 is installed on the top surface of the round buckling rod 9, a reset groove 12 is formed in one side of the installation groove 8, a fixing buckle 13 is slidably installed in the reset groove 12, a second spring 14 is installed on the fixing buckle 13, a push rod 16 is slidably installed in the pressing plate 7, a sliding groove 15 matched with the push rod 16 is formed in the interior of the pressing plate 7, the push rod 16 is fixedly connected with the fixing buckle 13, a blanking slide plate 17 is fixedly connected on one side of the cutting board 3, and the push rod 16 is slidably matched with the blanking slide plate 17; when in operation, the mechanical arm 2 drives the pressing plate 7 to move downwards, so that the pressing plate 7 is close to the chip plate 6 on the top surface of the base 1, the bottom of the round buckling rod 9 is inserted into the fixing hole 10 on the chip plate 6 to fix the chip plate 6, after the bottom end of the round buckling rod 9 contacts the top surface of the base 1, the round buckling rod 9 slides into the mounting groove 8, the first spring 11 is compressed, the top cone frustum of the round buckling rod 9 presses the fixing buckle 13, the fixing buckle 13 slides into the reset groove 12, the second spring 14 is compressed, after the top cone frustum of the round buckling rod 9 slides over the fixing buckle 13, the second spring 14 resets to push the fixing buckle 13 to clamp the round buckling rod 9, the round buckling rod 9 is fixed, the mechanical arm 2 drives the chip plate 6 to move to the cutting plate 3, the pushing pressure plate 7 moves downwards, the cooperation cutter 4 cuts the chip board 6, after the cutting is accomplished, the arm 2 drives the chip board 6 and moves to the unloading slide 17 department, make push rod 16 bump the lateral wall of unloading slide 17, make push rod 16 slide along spout 15, drive fixed knot 13 slide to reset inslot 12, release circle knot pole 9, circle knot pole 9 slides downwards under the elasticity effect of spring 11, circle knot pole 9 striking mounting groove 8's diapire, make circle knot pole 9 shake, shake down the chip board 6 to unloading slide 17, thereby the clamp of chip board 6 is got and the unloading work of waste material has been accomplished, the damage degree of clamping to chip board 6 has been reduced.
A straight notch 18 is formed in one surface of the pressing plate 7, which is close to the reset groove 12, the straight notch 18 is communicated with the reset groove 12, a sleeve seat 19 is slidably arranged in the reset groove 12, a fixing buckle 13 is slidably arranged in the sleeve seat 19, a second spring 14 is fixedly connected between the fixing buckle 13 and the inner wall of the sleeve seat 19, a long screw 20 is arranged on the sleeve seat 19, which is close to the straight notch 18, and the long screw 20 slidably penetrates through the straight notch 18; when the round buckle rod 9 works, the long screw rod 20 is loosened, the sleeve seat 19 is released, the long screw rod 20 is pushed to move along the straight slot 18, the sleeve seat 19 is driven to move in the reset slot 12, the position of the fixed buckle 13 in the reset slot 12 is adjusted, the sliding distance of the round buckle rod 9 is controlled, and then the operator can conveniently adjust the depth of the round buckle rod 9 inserted into the fixed hole 10.
As shown in fig. 5, a plurality of rubber strips 21 are arranged on the outer ring of the bottom of the round buckling rod 9, saw teeth are arranged on one side, away from the round buckling rod 9, of the rubber strips 21, and the outer walls of the plurality of rubber strips 21 are in sliding fit with the inner walls of the fixing holes 10; during operation, through the rubber strip 21 and the sawtooth of seting up, improved the friction force of circle knot pole 9 and fixed orifices 10, improved circle knot pole 9 and snatched the firm degree of chip board 6.
The middle part of the top surface of the mounting groove 8 is fixedly connected with a guide rod 23, a round hole 22 matched with the guide rod 23 is formed in the middle part of the round buckling rod 9, the outer ring of the guide rod 23 is sleeved on the inner ring of the first spring 11, a plurality of expansion grooves 24 are formed in the outer ring of the bottom of the round buckling rod 9, a rubber bar 21 is slidably mounted in the expansion grooves 24, a sleeve 25 is fixedly connected to the bottom of the round hole 22, a compression rod 26 is slidably mounted at the top of the sleeve 25, an elliptical marble 27 is fixedly connected between the bottom surface of the compression rod 26 and the bottom surface of the sleeve 25, an arc push plate 28 is slidably mounted on the outer ring of the sleeve 25, a plurality of connecting rods 29 are fixedly connected to one side, close to the inner wall of the sleeve 25, of the connecting rods 29 slidably penetrate through the outer wall of the sleeve 25, one ends, far away from the arc push plate 28, of the connecting rods 29 are fixedly connected with the rubber bar 21, a third spring 30 is sleeved on the outer ring of the connecting rods 29, and two ends of the third spring 30 are fixedly connected with the rubber bar 21 and the outer wall of the sleeve 25 respectively; when the device is in operation, after the bottom end of the round buckling rod 9 contacts the top surface of the base 1, the round buckling rod 9 slides into the mounting groove 8, the guide rod 23 slides into the round hole 22, the bottom end of the guide rod 23 pushes the compression rod 26 to slide into the sleeve 25, the oval marble 27 is extruded, the height of the oval marble 27 is reduced, the diameter is expanded, the arc-shaped push plate 28 is pushed to slide into the inner wall of the sleeve 25, the connecting rod 29 is pushed to push the rubber strip block 21 to slide out of the expansion groove 24, and the plurality of rubber strip blocks 21 are unfolded in the fixing hole 10, so that the stability of the round buckling rod 9 for grabbing the chip board 6 is further improved.
As shown in fig. 6, a rubber plate 31 is slidably mounted in a groove on the top surface of the cutting plate 3, a circular groove of the rubber plate 31 is sleeved on the outer ring of the cutter 4, a plurality of fourth springs 32 are fixedly connected to the bottom surface of the rubber plate 31, and the bottom ends of the fourth springs 32 are fixedly connected to the top surface of the cutting plate 3; during operation, the mechanical arm 2 drives the pressing plate 7 and the chip plate 6 to move into the groove on the top surface of the cutting plate 3, the pressing plate 7 pushes the chip plate 6 to move downwards, the chip plate 6 is clamped and fixed by matching with the rubber plate 31, and the stability of the cutting knife 4 when cutting the chip plate 6 is improved.
A plurality of air cavities 33 are formed in the rubber plate 31, one end of each air cavity 33 is communicated with a round groove of the rubber plate 31, and a plurality of vertical grooves are formed in the cutter 4; when the device works, when the pressing plate 7 presses the rubber plate 31, the rubber plate 31 slides downwards, the rubber plate 31 compresses, gas in the air cavity 33 is pressed into the circular groove of the rubber plate 31, the cut biochip is blown, the smoothness of the biochip falling is improved, and the probability of the biochip blocking the blanking pipe 5 is reduced.
Example two
As shown in fig. 7, in comparative example one, another embodiment of the present invention is: a knife stone 34 is fixedly connected to the inner wall of the circular groove of the rubber plate 31, and the knife stone 34 is in sliding fit with the cutter 4; when the cutter knife is in operation, the rubber plate 31 slides to drive the knife stone 34 to be attached to the cutter knife 4 to slide, and the cutter knife 4 is polished, so that the sharpness of the cutter knife 4 is improved, and the cutting efficiency of the cutter knife 4 to the chip plate 6 is improved.
When in operation, the device comprises: the mechanical arm 2 drives the pressing plate 7 to move downwards, so that the pressing plate 7 is close to the chip plate 6 on the top surface of the base 1, the bottom of the round buckling rod 9 is inserted into the fixing hole 10 on the chip plate 6, when the bottom end of the round buckling rod 9 contacts the top surface of the base 1, the round buckling rod 9 slides into the mounting groove 8, the first spring 11 is compressed, the top cone frustum of the round buckling rod 9 extrudes the fixing buckle 13, the fixing buckle 13 slides into the reset groove 12, the second spring 14 is compressed, and when the top cone frustum of the round buckling rod 9 slides over the fixing buckle 13, the second spring 14 resets to push the fixing buckle 13 to clamp the round buckling rod 9, and the round buckling rod 9 is fixed; the guide rod 23 slides into the round hole 22, the bottom end of the guide rod 23 pushes the compression bar 26 to slide into the sleeve 25, the oval marble 27 is extruded, the oval marble 27 is reduced in height and expanded in diameter, the arc push plate 28 is pushed to slide to the inner wall of the sleeve 25, the connecting rod 29 is pushed to push the rubber strip block 21 to slide out of the expansion groove 24, the plurality of rubber strip blocks 21 are unfolded in the fixing hole 10, and the chip board 6 is fixed to the bottom of the pressing plate 7;
the mechanical arm 2 drives the pressing plate 7 and the chip plate 6 to move to the cutting plate 3, the pressing plate 7 is pushed to move downwards, the chip plate 6 is pushed into a groove on the top surface of the cutting plate 3, the chip plate 6 contacts with the rubber plate 31, the biochip on the chip plate 6 is positioned in the middle of the cutter 4, the pressing plate 7 moves downwards, the rubber plate 31 slides downwards, the fourth spring 32 is compressed, the chip plate 6 is cut by matching with the cutter 4, the cut biochip falls into the blanking pipe 5, and is individually packaged by a packaging bag at the bottom of the blanking pipe 5; the mechanical arm 2 drives the cut chip board 6 to move to the blanking slide plate 17, the push rod 16 collides with the side wall of the blanking slide plate 17, so that the push rod 16 slides along the slide groove 15, the fixing buckle 13 is driven to slide into the reset groove 12, the round buckle rod 9 is released, the round buckle rod 9 slides downwards under the action of the elastic force of the first spring 11, the round buckle rod 9 collides with the bottom wall of the mounting groove 8, the round buckle rod 9 vibrates, meanwhile, the guide rod 23 slides into the round hole 22, so that the oval marble 27 is reset, the third spring 30 resets, the rubber bar 21 is driven to slide into the expansion groove 24, the chip board 6 is released, and the chip board 6 falls onto the blanking slide plate 17; thus, the individual packaging of the biochip is completed, and the operator can conveniently select the biochip with flaws.
The front, rear, left, right, up and down are all based on fig. 1 in the drawings of the specification, the face of the device facing the observer is defined as front, the left side of the observer is defined as left, and so on, according to the viewing angle of the person.
In the description of the present invention, it should be understood that the terms "center," "longitudinal," "lateral," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present invention and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the scope of the present invention.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A microfluidic biochip for nucleic acid detection, characterized in that: comprises a chip body (35) and a sealing cover (36); the chip comprises a chip body (35), wherein a hole channel is formed in the chip body (35), a sample adding port is formed in one end of the chip body (35), the sample adding port is communicated with the hole channel, a nano fluorescent probe is arranged at one end, close to the hole channel, of the sample adding port, a sealing cover (36) is fixedly connected to the side face of one end, close to the sample adding port, of the chip body (35), and the outer wall of the sealing cover (36) is in threaded fit with the inner wall of the sample adding port;
the production process of the microfluidic biochip comprises the following steps:
s1: adding the adhesive A and the adhesive B into a vacuum defoaming stirrer according to the proportion of 10:1, and uniformly stirring and mixing in vacuum;
s2: adding the stirred and mixed glue into a forming die, performing injection molding, and cooling and solidifying the chip board (6);
s3: moving the chip board (6) onto a conveyor belt, punching the chips, and then cleaning, sterilizing and drying the chips;
s4: the chip board (6) is conveyed to the blanking device by a conveyor belt, the chip board (6) conveyed to the top of the base (1) is grabbed by the mechanical arm (2), the chip board (6) is conveyed to the top of the cutting board (3), the biological chips on the chip board (6) are cut off by the cutter (4), and the biological chips are separated and dropped by the corresponding blanking pipes (5) to be scattered and packaged;
the blanking device in the S4 comprises a base (1), a mechanical arm (2), a grabbing structure, a cutting plate (3), a cutter (4) and a blanking pipe (5); the automatic feeding device is characterized in that a chip board (6) is placed on a top surface conveyor belt of the base (1), one side of the base (1) is provided with a mechanical arm (2), one end, away from the base (1), of the mechanical arm (2) is provided with a grabbing structure, one side, away from the mechanical arm (2), of the base (1) is fixedly connected with a cutting board (3), the bottom surface of the cutting board (3) is fixedly connected with a plurality of blanking pipes (5), the blanking pipes (5) penetrate through the cutting board (3), packaging bags are sleeved on the outer ring of the blanking pipes (5), the top surface of the cutting board (3) is fixedly connected with a cutter (4), and the inner ring of the cutter (4) is sleeved on the inner ring of the blanking pipe (5);
the grabbing structure comprises a pressing plate (7) and a round buckling rod (9), the bottom end of the mechanical arm (2) is fixedly connected with the pressing plate (7), a plurality of mounting grooves (8) are formed in the bottom surface of the pressing plate (7), the round buckling rod (9) is slidably mounted in the mounting grooves (8), a conical frustum is arranged at the top of the round buckling rod (9), a fixing hole (10) matched with the round buckling rod (9) is formed in the middle of the chip board (6), a spring (11) is mounted on the top surface of the round buckling rod (9), a reset groove (12) is formed in one side of the mounting groove (8), a fixing buckle (13) is slidably mounted in the reset groove (12), a second spring (14) is mounted on the fixing buckle (13), a push rod (16) is slidably mounted in the pressing plate (7), a slide groove (15) matched with the push rod (16) is formed in the pressing plate (7), a first side of the cutting board (3) is fixedly connected with the fixing buckle (13), and a blanking slide plate (17) is slidably mounted on the sliding plate (17).
The bottom outer ring of circle knot pole (9) is provided with a plurality of rubber strip piece (21), the sawtooth has been seted up to one side that circle knot pole (9) was kept away from to rubber strip piece (21), a plurality of the outer wall of rubber strip piece (21) and the inner wall sliding fit of fixed orifices (10).
2. The process for producing a microfluidic biochip for nucleic acid detection according to claim 1, wherein: the utility model discloses a clamp plate, including clamp plate (7), straight notch (18) have been seted up to the one side that clamp plate (7) is close to return groove (12), straight notch (18) intercommunication return groove (12), the inside slidable mounting in return groove (12) has cover seat (19), the inside slidable mounting of cover seat (19) has fixed knot (13), the rigid coupling has No. two springs (14) between the inner wall of fixed knot (13) and cover seat (19), the screw thread that cover seat (19) is close to straight notch (18) is installed long screw rod (20), long screw rod (20) slip runs through straight notch (18).
3. The process for producing a microfluidic biochip for nucleic acid detection according to claim 1, wherein: the utility model discloses a rubber strip piece, including installation groove (8), guide arm (23) have been seted up at the top surface middle part rigid coupling of mounting groove (8), round buckle the middle part of pole (9) offered with guide arm (23) assorted round hole (22), the outer lane cover of guide arm (23) is at the inner circle of spring (11) No. one, a plurality of expansion grooves (24) have been seted up to the bottom outer lane of round buckle pole (9), the inside slidable mounting of expansion groove (24) has rubber strip piece (21), the bottom rigid coupling of round hole (22) has sleeve (25), the top slidable mounting of sleeve (25) has depression bar (26), rigid coupling has oval marble (27) between the bottom surface of depression bar (26) and the bottom surface of sleeve (25), the outer lane slidable mounting of sleeve (25) has arc push pedal (28), one side rigid coupling that arc push pedal (28) is close to sleeve (25) inner wall has a plurality of connecting rods (29), the one end and rubber strip piece (21) rigid coupling that arc push pedal (28) were kept away from to connecting rod (29), the outer ring (30) of connecting rod (29) are equipped with spring No. three and sleeve (30) respectively.
4. The process for producing a microfluidic biochip for nucleic acid detection according to claim 1, wherein: the novel cutting device is characterized in that a rubber plate (31) is slidably mounted in a groove on the top surface of the cutting plate (3), the circular groove of the rubber plate (31) is sleeved with an outer ring of the cutter (4), the bottom surface of the rubber plate (31) is fixedly connected with a plurality of No. four springs (32), and the bottom end of the No. four springs (32) is fixedly connected with the top surface of the cutting plate (3).
5. The process for producing a microfluidic biochip for nucleic acid detection according to claim 4, wherein: a plurality of air cavities (33) are formed in the rubber plate (31), one end of each air cavity (33) is communicated with a round groove of the rubber plate (31), and a plurality of vertical grooves are formed in the cutter (4).
6. The process for producing a microfluidic biochip for nucleic acid detection according to claim 4, wherein: the inner wall of the circular groove of the rubber plate (31) is fixedly connected with a knife stone (34), and the knife stone (34) is in sliding fit with the cutter (4).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210065415.1A CN114405564B (en) | 2022-01-19 | 2022-01-19 | Microfluidic biochip for detecting nucleic acid and production process |
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| CN202210065415.1A CN114405564B (en) | 2022-01-19 | 2022-01-19 | Microfluidic biochip for detecting nucleic acid and production process |
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| CN114405564B true CN114405564B (en) | 2023-05-09 |
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| JP2001083208A (en) * | 1999-09-13 | 2001-03-30 | Nec Corp | Prober for analyzing semiconductor chip and semiconductor chip analyzer |
| JP2006181945A (en) * | 2004-12-28 | 2006-07-13 | Daiichi Seiko Kk | Insert shaping apparatus, terminal material for insert shaping, and insert shaping method |
| CN210294029U (en) * | 2019-03-11 | 2020-04-10 | 徐州医科大学 | Biochip detector |
| CN213121989U (en) * | 2020-07-30 | 2021-05-04 | 深圳中科云信息技术有限公司 | Chip detector with protection architecture |
| CN214818631U (en) * | 2021-08-02 | 2021-11-23 | 滁州市精美家电设备股份有限公司 | Manipulator of electronic product production line |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107159330A (en) * | 2017-06-09 | 2017-09-15 | 北京百康芯生物科技有限公司 | A kind of diaphragm type nucleic acid amplification micro-fluidic chip and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001083208A (en) * | 1999-09-13 | 2001-03-30 | Nec Corp | Prober for analyzing semiconductor chip and semiconductor chip analyzer |
| JP2006181945A (en) * | 2004-12-28 | 2006-07-13 | Daiichi Seiko Kk | Insert shaping apparatus, terminal material for insert shaping, and insert shaping method |
| CN210294029U (en) * | 2019-03-11 | 2020-04-10 | 徐州医科大学 | Biochip detector |
| CN213121989U (en) * | 2020-07-30 | 2021-05-04 | 深圳中科云信息技术有限公司 | Chip detector with protection architecture |
| CN214818631U (en) * | 2021-08-02 | 2021-11-23 | 滁州市精美家电设备股份有限公司 | Manipulator of electronic product production line |
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| CN114405564A (en) | 2022-04-29 |
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