CN114404516A - Fresh polygonatum freeze-dried decoction pieces and preparation method thereof - Google Patents
Fresh polygonatum freeze-dried decoction pieces and preparation method thereof Download PDFInfo
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- 241000756042 Polygonatum Species 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 239000012043 crude product Substances 0.000 claims abstract description 51
- 238000000855 fermentation Methods 0.000 claims abstract description 51
- 230000004151 fermentation Effects 0.000 claims abstract description 51
- 108010068005 Oxalate decarboxylase Proteins 0.000 claims abstract description 24
- 238000004108 freeze drying Methods 0.000 claims abstract description 24
- 235000015112 vegetable and seed oil Nutrition 0.000 claims abstract description 14
- 239000008158 vegetable oil Substances 0.000 claims abstract description 14
- 239000012535 impurity Substances 0.000 claims abstract description 11
- 230000002378 acidificating effect Effects 0.000 claims abstract description 10
- 238000007654 immersion Methods 0.000 claims abstract description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 75
- 238000007710 freezing Methods 0.000 claims description 30
- 230000008014 freezing Effects 0.000 claims description 30
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 18
- 238000001035 drying Methods 0.000 claims description 15
- 238000002791 soaking Methods 0.000 claims description 13
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 12
- 241001633680 Polygonatum odoratum Species 0.000 claims description 10
- 238000004140 cleaning Methods 0.000 claims description 10
- 238000005520 cutting process Methods 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims description 9
- POLIXZIAIMAECK-UHFFFAOYSA-N 4-[2-(2,6-dioxomorpholin-4-yl)ethyl]morpholine-2,6-dione Chemical compound C1C(=O)OC(=O)CN1CCN1CC(=O)OC(=O)C1 POLIXZIAIMAECK-UHFFFAOYSA-N 0.000 claims description 5
- GTDPSWPPOUPBNX-UHFFFAOYSA-N ac1mqpva Chemical compound CC12C(=O)OC(=O)C1(C)C1(C)C2(C)C(=O)OC1=O GTDPSWPPOUPBNX-UHFFFAOYSA-N 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- QXDMQSPYEZFLGF-UHFFFAOYSA-L calcium oxalate Chemical compound [Ca+2].[O-]C(=O)C([O-])=O QXDMQSPYEZFLGF-UHFFFAOYSA-L 0.000 abstract description 14
- 229940126680 traditional chinese medicines Drugs 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 34
- 230000000052 comparative effect Effects 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 239000013078 crystal Substances 0.000 description 6
- 239000008159 sesame oil Substances 0.000 description 5
- 235000011803 sesame oil Nutrition 0.000 description 5
- 238000000859 sublimation Methods 0.000 description 4
- 230000008022 sublimation Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 3
- 235000019483 Peanut oil Nutrition 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 229910001424 calcium ion Inorganic materials 0.000 description 3
- 239000002285 corn oil Substances 0.000 description 3
- 235000005687 corn oil Nutrition 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 235000008216 herbs Nutrition 0.000 description 3
- 235000006408 oxalic acid Nutrition 0.000 description 3
- 239000000312 peanut oil Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 3
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 3
- 241000183294 Scleropages formosus Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000019605 sweet taste sensations Nutrition 0.000 description 2
- 230000035922 thirst Effects 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 206010013789 Dry throat Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000001055 chewing effect Effects 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000005550 inflammation mediator Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 230000001050 lubricating effect Effects 0.000 description 1
- 238000005461 lubrication Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000006011 modification reaction Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8969—Polygonatum (Solomon's seal)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
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- Epidemiology (AREA)
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- General Health & Medical Sciences (AREA)
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- Veterinary Medicine (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses fresh polygonatum freeze-dried decoction pieces and a preparation method thereof, and relates to the field of traditional Chinese medicines, the fresh polygonatum is cleaned and subjected to impurity removal, then the fresh polygonatum is cut into thick pieces, the thick pieces are placed into an acidic soak solution containing vegetable oil for ultrasonic immersion, and the thick pieces are taken out and dried to obtain a first crude product; putting the first crude product into fermentation liquor containing oxalate decarboxylase for fermentation, and obtaining a second crude product after the fermentation is finished; and (4) carrying out freeze drying on the second crude product under the vacuum condition to obtain a third crude product. According to the fresh polygonatum freeze-dried decoction pieces, the content of calcium oxalate in the fresh polygonatum freeze-dried decoction pieces is reduced, and meanwhile, the dried decoction pieces are not easy to crack and are easy to store.
Description
Technical Field
The invention relates to the field of traditional Chinese medicines, in particular to a fresh polygonatum freeze-dried decoction piece and a preparation method thereof.
Background
The polygonatum is a dried rhizome of the polygonatum which is a liliaceous plant and is in a long cylindrical shape, is slightly flat, has few branches, is 4-18cm long, has the diameter of 0.3-1.6cm, has yellowish white or yellowish brown surface, is semitransparent, has links of longitudinal wrinkles and micro bulges, has white round fibrous root marks and disc-shaped stem marks, is hard, crisp or slightly soft, is easy to break, has a cutin-shaped cross section or is granular, has slight smell, sweet taste, sticky chewing, sweet taste and slight cold; entering lung and stomach meridians; nourishing yin, moistening dryness, promoting fluid production, and quenching thirst; can be used for treating yin injury of lung and stomach, cough due to dryness-heat, dry throat, thirst, and diabetes due to internal heat.
The polygonatum contains abundant polygonatum polysaccharides and other substances, wherein the polygonatum polysaccharides have various effects of reducing blood sugar, resisting aging, resisting lipid peroxidation and the like, but the polygonatum also contains a large amount of fixed calcium oxalate needle crystals which can directly stimulate mucous membrane cells to cause cell damage, thereby generating a large amount of inflammation mediators, causing irritation and even pain, so that the calcium oxalate needle crystals in the polygonatum need to be removed as much as possible in the processing process of the polygonatum.
Disclosure of Invention
The invention aims to at least solve one of the technical problems in the prior art and provides a fresh polygonatum freeze-dried decoction piece and a preparation method thereof.
The technical solution of the invention is as follows:
a fresh polygonatum freeze-dried decoction piece comprises the following raw materials: fresh polygonatum odoratum, an acidic soak solution containing vegetable oil and a fermentation solution containing oxalate decarboxylase.
Preferably, the acidic soaking solution further comprises an acetic acid solution.
Preferably, the mass ratio of the vegetable oil to the acetic acid solution in the acidic soaking solution is 1: 1-3.
The invention also discloses a preparation method of the fresh polygonatum freeze-dried decoction pieces, which comprises the following steps:
the method comprises the following steps: cleaning fresh radix polygonati officinalis, removing impurities, cutting into thick slices, putting the thick slices into an acidic soaking solution containing vegetable oil, performing ultrasonic immersion, taking out, and drying to obtain a first crude product;
step two: putting the first crude product into fermentation liquor containing oxalate decarboxylase for fermentation, and obtaining a second crude product after the fermentation is finished;
step three: freeze-drying the second crude product under vacuum condition to obtain a third crude product,
preferably, the preparation method of the fermentation liquor comprises the following steps: dissolving oxalate decarboxylase in an ethylenediamine tetraacetic dianhydride solution, adjusting the pH value to 5-6, and modifying at 25-35 ℃ for 4-10h to obtain the oxalate dianhydride.
Preferably, the conditions of freeze-drying are: freezing at-40 to-30 ℃ for 1 to 2 hours, freezing at-30 to-20 ℃ for 1 to 2 hours, freezing at-20 to 0 ℃ for 1 to 2 hours, heating to 20 to 30 ℃, and preserving heat for 0.5 to 2 hours.
Preferably, the thickness of the slab is 0.6-1 mm.
Preferably, in the first step, the drying is carried out in a nitrogen-filled oven, and the drying air speed is 8-9 m/s.
The invention has the beneficial effects that:
(1) according to the fresh polygonatum freeze-dried decoction pieces, the content of calcium oxalate in the fresh polygonatum freeze-dried decoction pieces is reduced, and meanwhile, the dried decoction pieces are not easy to crack and are easy to store.
(2) According to the preparation method of the fresh polygonatum odoratum freeze-dried decoction pieces, thick pieces with certain thickness are cut, vegetable oil is mixed in the acidic soak solution, calcium oxalate needle crystals contained in the polygonatum odoratum are dissolved into oxalic acid and calcium ions, and then fermentation is carried out in the fermentation liquor of oxalate decarboxylase to form a multienzyme acid solution, so that the calcium oxalate is degraded, the calcium oxalate needle crystals are reduced to remain in the polygonatum odoratum decoction pieces, and the harm to the practicality of a human body is avoided; on the other hand simultaneously with vegetable oil molecule supersound attached to the space and the surface of polygonatum, at freeze-drying's in-process, carry out dehydration along with the sublimation of hydrone, the sliced medicinal herbs can appear certain deformation curling, the existence of vegetable oil can slow down the speed that moisture sublimes to a certain extent, on the other hand has certain softening and lubrication action to the sliced medicinal herbs surface after the drying, is difficult for embrittling, provides certain stability and convenience to sliced medicinal herbs storage or transportation.
Detailed Description
This section will describe in detail specific embodiments of the invention, which should not be construed as limiting the scope of the invention.
In the following examples:
sesame oil: brand name: taitera, unsaturated fatty acid 83 wt%;
peanut oil: brand name: the content of unsaturated fatty acid in the golden dragon fish is 84 wt%;
corn oil: brand name: 85 wt% of golden dragon fish and unsaturated fatty acid.
Example 1
A preparation method of fresh polygonatum freeze-dried decoction pieces comprises the following steps:
the method comprises the following steps: cleaning fresh rhizoma Polygonati Odorati, removing impurities, cutting into 0.6mm thick slices, ultrasonic soaking in acetic acid solution containing oleum Sesami, taking out, and drying at 8m/s in nitrogen-filled oven; obtaining a first crude product; the mass ratio of the sesame oil to the acetic acid solution is 1:1, and the concentration of the acetic acid solution is 10 wt%;
step two: putting the first crude product into fermentation liquor containing oxalate decarboxylase for fermentation for 10 hours, and filtering to obtain a second crude product after the fermentation is finished;
step three: freeze-drying the second crude product under vacuum condition to obtain a third crude product;
the preparation method of the fermentation liquor comprises the following steps: oxalate decarboxylase is dissolved in water to prepare 0.05 wt% fermentation liquor.
The conditions of freeze drying are as follows: freezing at-35 deg.C for 1h, freezing at-25 deg.C for 2h, freezing at 0 deg.C for 1.5h, heating to 25 deg.C, and maintaining for 1 h.
Example 2
A preparation method of fresh polygonatum freeze-dried decoction pieces comprises the following steps:
the method comprises the following steps: cleaning fresh rhizoma Polygonati Odorati, removing impurities, cutting into 0.6mm thick slices, ultrasonic soaking in acetic acid solution containing oleum Sesami, taking out, and drying at 9m/s in nitrogen-filled oven; obtaining a first crude product; the mass ratio of the sesame oil to the acetic acid solution is 1:2, and the concentration of the acetic acid solution is 10 wt%;
step two: putting the first crude product into fermentation liquor containing oxalate decarboxylase for fermentation, and obtaining a second crude product after the fermentation is finished;
step three: freeze-drying the second crude product under vacuum condition to obtain a third crude product;
the preparation method of the fermentation liquor comprises the following steps: oxalate decarboxylase is dissolved in water to prepare 0.08 wt% fermentation liquor.
The conditions of freeze drying are as follows: freezing at-35 deg.C for 1h, freezing at-25 deg.C for 2h, freezing at 0 deg.C for 1.5h, heating to 25 deg.C, and maintaining for 1 h.
Example 3
A preparation method of fresh polygonatum freeze-dried decoction pieces comprises the following steps:
the method comprises the following steps: cleaning fresh rhizoma Polygonati Odorati, removing impurities, cutting into 0.8mm thick slices, ultrasonic soaking in peanut oil-containing acetic acid solution, taking out, and drying at 9m/s in nitrogen-filled oven; obtaining a first crude product; the mass ratio of the peanut oil to the acetic acid solution is 1:3, and the concentration of the acetic acid solution is 12 wt%;
step two: putting the first crude product into fermentation liquor containing oxalate decarboxylase for fermentation, and obtaining a second crude product after the fermentation is finished;
step three: freeze-drying the second crude product under vacuum condition to obtain a third crude product;
the preparation method of the fermentation liquor comprises the following steps: oxalate decarboxylase is dissolved in water to prepare 0.08 wt% fermentation liquor.
The conditions of freeze drying are as follows: freezing at-35 deg.C for 1h, freezing at-25 deg.C for 2h, freezing at 0 deg.C for 1.5h, heating to 25 deg.C, and maintaining for 1 h.
Example 4
A preparation method of fresh polygonatum freeze-dried decoction pieces comprises the following steps:
the method comprises the following steps: cleaning fresh rhizoma Polygonati Odorati, removing impurities, cutting into 1mm thick slices, ultrasonic soaking in acetic acid solution containing corn oil, taking out, and drying at 8m/s in nitrogen-filled oven; obtaining a first crude product; the mass ratio of the corn oil to the acetic acid solution is 1:3, and the concentration of the acetic acid solution is 12 wt%;
step two: putting the first crude product into fermentation liquor containing oxalate decarboxylase for fermentation, and obtaining a second crude product after the fermentation is finished;
step three: freeze-drying the second crude product under vacuum condition to obtain a third crude product;
the preparation method of the fermentation liquor comprises the following steps: oxalate decarboxylase is dissolved in water to prepare 0.08 wt% fermentation liquor.
The conditions of freeze drying are as follows: freezing at-35 deg.C for 1h, freezing at-25 deg.C for 2h, freezing at 0 deg.C for 1.5h, heating to 25 deg.C, and maintaining for 1 h.
Example 5
A preparation method of fresh polygonatum freeze-dried decoction pieces comprises the following steps:
the method comprises the following steps: cleaning fresh rhizoma Polygonati Odorati, removing impurities, cutting into 0.6mm thick slices, ultrasonic soaking in acetic acid solution containing oleum Sesami, taking out, and drying at 8m/s in nitrogen-filled oven; obtaining a first crude product; the mass ratio of the sesame oil to the acetic acid solution is 1:3, and the concentration of the acetic acid solution is 10 wt%;
step two: putting the first crude product into fermentation liquor containing oxalate decarboxylase for fermentation, and obtaining a second crude product after the fermentation is finished;
step three: freeze-drying the second crude product under vacuum condition to obtain a third crude product;
the preparation method of the fermentation liquor comprises the following steps: dissolving oxalate decarboxylase in an ethylenediamine tetraacetic dianhydride solution, adjusting the pH value to 5.5, and performing modification reaction at 25 ℃ for 8 hours to obtain the oxalate dianhydride and ethylenediamine tetraacetic dianhydride mixed solution, wherein the quantity ratio of the added substances of the oxalate decarboxylase to the ethylenediamine tetraacetic dianhydride is 1: 62.
The conditions of freeze drying are as follows: freezing at-35 deg.C for 1h, freezing at-25 deg.C for 2h, freezing at 0 deg.C for 1.5h, heating to 25 deg.C, and maintaining for 1 h.
Comparative example 1 (one-off temperature-rising freeze drying)
A preparation method of fresh polygonatum freeze-dried decoction pieces comprises the following steps:
the method comprises the following steps: cleaning fresh rhizoma Polygonati Odorati, removing impurities, cutting into 0.6mm thick slices, ultrasonic soaking in acetic acid solution containing oleum Sesami, taking out, and drying at 8m/s in nitrogen-filled oven; obtaining a first crude product; the mass ratio of the sesame oil to the acetic acid solution is 1:1, and the concentration of the acetic acid solution is 10 wt%;
step two: putting the first crude product into fermentation liquor containing oxalate decarboxylase for fermentation for 10 hours, and filtering to obtain a second crude product after the fermentation is finished;
step three: freeze-drying the second crude product under vacuum condition to obtain a third crude product;
the preparation method of the fermentation liquor comprises the following steps: oxalate decarboxylase is dissolved in water to prepare 0.05 wt% fermentation liquor.
The conditions of freeze drying are as follows: freezing at-35 deg.C for 3.5h, heating to 25 deg.C, and maintaining for 1 h.
Comparative example 2 (No vegetable oil)
A preparation method of fresh polygonatum freeze-dried decoction pieces comprises the following steps:
the method comprises the following steps: cleaning fresh rhizoma Polygonati Odorati, removing impurities, cutting into 0.6mm thick slices, ultrasonic soaking in acetic acid solution, taking out, and drying at 8m/s in nitrogen-filled oven; obtaining a first crude product; the concentration of the acetic acid solution is 10 wt%;
step two: putting the first crude product into fermentation liquor containing oxalate decarboxylase for fermentation for 10 hours, and filtering to obtain a second crude product after the fermentation is finished;
step three: freeze-drying the second crude product under vacuum condition to obtain a third crude product;
the preparation method of the fermentation liquor comprises the following steps: oxalate decarboxylase is dissolved in water to prepare 0.05 wt% fermentation liquor.
The conditions of freeze drying are as follows: freezing at-35 deg.C for 1h, freezing at-25 deg.C for 2h, freezing at 0 deg.C for 1.5h, heating to 25 deg.C, and maintaining for 1 h.
Comparative example 3 (acetic acid free and fermentation broth)
A preparation method of fresh polygonatum freeze-dried decoction pieces comprises the following steps:
cleaning fresh rhizoma Polygonati Odorati, removing impurities, cutting into 0.6mm thick slices, and freeze drying under vacuum condition to obtain third crude product;
the conditions of freeze drying are as follows: freezing at-35 deg.C for 1h, freezing at-25 deg.C for 2h, freezing at 0 deg.C for 1.5h, heating to 25 deg.C, and maintaining for 1 h.
The above examples and comparative examples were subjected to the performance test, and the test values are shown in Table 1.
The calcium oxalate content was determined by high performance liquid chromatography (HPLC method).
c, brittle fracture rate test: and (3) vibrating the sample on a vibrating screen for 30min at a vibration frequency of 50HZ, and testing the friability rate, namely the mass of the fragments which are vibrated off/the mass of the original sample.
| Test specimen | Calcium oxalate content (%). before processing | Calcium oxalate content after processing (%) | Friability (%) |
| Example 1 | 0.0084±0.0003 | 0.0025±0.0002 | 0.5 |
| Example 2 | 0.0086±0.0005 | 0.0024±0.0003 | 0.6 |
| Example 3 | 0.0082±0.0004 | 0.0023±0.0005 | 0.4 |
| Example 4 | 0.0085±0.0003 | 0.0022±0.0001 | 0.5 |
| Example 5 | 0.0086±0.0004 | 0.0019±0.0002 | 0.4 |
| Comparative example 1 | 0.0085±0.0002 | 0.0028±0.0003 | 1.2 |
| Comparative example 2 | 0.0084±0.0003 | 0.0029±0.0004 | 1.5 |
| Comparative example 3 | 0.0082±0.0003 | 0.0036±0.0001 | 1.6 |
From the above table, the performance of the examples is superior to that of the comparative examples, and the main reason may be as follows, as can be seen from the analysis of the comparative example 1, the multi-stage freezing sublimation is performed in the examples, so that the dehydration rate is reduced in the freezing and drying process, the contraction rate of the polygonatum odoratum is reduced, the deformation amount is reduced, the loose porous structure of the decoction pieces is more uniform, and the distribution of the vegetable oil is more uniform; the analysis of the comparative example 2 shows that the vegetable oil molecules are ultrasonically attached to the gaps and the surfaces of the polygonatum odoratum, the decoction pieces are subjected to certain deformation and curling along with the sublimation of the water molecules during the freeze drying process, the sublimation rate of water can be slowed down to a certain extent by the presence of the vegetable oil, and the dried decoction pieces have certain softening and lubricating effects on the surfaces, are not easy to embrittle and provide certain stability and convenience for the storage or transportation of the decoction pieces; the analysis of comparative example 3 shows that the calcium oxalate needle crystals contained in the polygonatum odoratum are dissolved into oxalic acid and calcium ions, and then the oxalic acid and the calcium ions are fermented in the fermentation liquid to form a multienzyme acid solution, so that the calcium oxalate is degraded, the calcium oxalate needle crystals are reduced to remain in the polygonatum odoratum decoction pieces, the harm to the practicality of a human body is avoided, meanwhile, the performance of the embodiment 5 is optimal, and mainly due to the fact that the oxalate decarboxylase is modified, the binding sites of the modified enzyme to the calcium oxalate are increased, and the degradation rate is increased.
The above additional technical features can be freely combined and used in superposition by those skilled in the art without conflict.
The above description is only a preferred embodiment of the present invention, and the technical solutions that achieve the objects of the present invention by substantially the same means are within the protection scope of the present invention.
Claims (8)
1. The fresh polygonatum freeze-dried decoction pieces are characterized by comprising the following raw materials: fresh polygonatum odoratum, an acidic soak solution containing vegetable oil and a fermentation solution containing oxalate decarboxylase.
2. The fresh radix polygonati officinalis freeze-dried decoction pieces according to claim 1, wherein the acidic soaking solution further comprises an acetic acid solution.
3. The fresh polygonatum odoratum freeze-dried decoction pieces as claimed in claim 2, wherein the mass ratio of the vegetable oil to the acetic acid solution in the acidic soaking solution is 1: 1-3.
4. The preparation method of the fresh polygonatum freeze-dried decoction pieces is characterized by comprising the following steps:
the method comprises the following steps: cleaning fresh radix polygonati officinalis, removing impurities, cutting into thick slices, putting the thick slices into an acidic soaking solution containing vegetable oil, performing ultrasonic immersion, taking out, and drying to obtain a first crude product;
step two: putting the first crude product into fermentation liquor containing oxalate decarboxylase for fermentation, and obtaining a second crude product after the fermentation is finished;
step three: and (4) carrying out freeze drying on the second crude product under the vacuum condition to obtain a third crude product.
5. The method for preparing fresh radix polygonati officinalis freeze-dried decoction pieces according to claim 4, wherein the method for preparing the fermentation liquid comprises the following steps: dissolving oxalate decarboxylase in an ethylenediamine tetraacetic dianhydride solution, adjusting the pH value to 5-6, and modifying at 25-35 ℃ for 4-10h to obtain the oxalate dianhydride.
6. The method for preparing fresh radix polygonati officinalis freeze-dried decoction pieces according to claim 4, wherein the freeze-drying conditions are as follows: freezing for 1-2h at-40 to-30 ℃, freezing for 1-2h at-30 to-20 ℃, freezing for 1-2h at-20 to 0 ℃, heating to 20-30 ℃, and preserving heat for 0.5-2 h.
7. The method for preparing fresh radix polygonati officinalis freeze-dried decoction pieces according to claim 4, wherein the thickness of the thick pieces is 0.6-1 mm.
8. The method for preparing fresh radix polygonati officinalis freeze-dried decoction pieces according to claim 4, wherein in the first step, the drying is carried out in a nitrogen-filled oven at a drying speed of 8-9 m/s.
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| CN116138448A (en) * | 2022-09-07 | 2023-05-23 | 徐玉刚 | Preparation method of instant freeze-dried radix polygonati officinalis slices |
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| CN109157606A (en) * | 2018-07-09 | 2019-01-08 | 湖南神舟中药饮片有限公司 | A kind of preparation method of Chinese medicine radix polygonati officinalis medicine materical crude slice |
| CN111388597A (en) * | 2020-03-25 | 2020-07-10 | 安徽益健堂中药饮片科技有限公司 | Preparation process of traditional Chinese medicine radix polygonati officinalis decoction pieces |
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| CN116138448A (en) * | 2022-09-07 | 2023-05-23 | 徐玉刚 | Preparation method of instant freeze-dried radix polygonati officinalis slices |
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