CN114402066A - 使用离子浓度提高重组腺相关病毒的包装效率 - Google Patents

使用离子浓度提高重组腺相关病毒的包装效率 Download PDF

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Publication number
CN114402066A
CN114402066A CN202080064369.2A CN202080064369A CN114402066A CN 114402066 A CN114402066 A CN 114402066A CN 202080064369 A CN202080064369 A CN 202080064369A CN 114402066 A CN114402066 A CN 114402066A
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raav
cells
aav
medium
production
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王启钊
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Charles River Laboratories Inc
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Charles River Laboratories Inc
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
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    • C12N5/16—Animal cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
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    • C12N2500/16—Magnesium; Mg chelators
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    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
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    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
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    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14311—Parvovirus, e.g. minute virus of mice
    • C12N2750/14351—Methods of production or purification of viral material

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  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN202080064369.2A 2019-07-15 2020-08-12 使用离子浓度提高重组腺相关病毒的包装效率 Pending CN114402066A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16/511,612 US10801042B1 (en) 2019-07-15 2019-07-15 Use of ion concentrations to increase the packaging efficiency of recombinant adeno-associated virus
PCT/US2020/045906 WO2021011941A1 (en) 2019-07-15 2020-08-12 Use of ion concentrations to increase the packaging efficiency of recombinant adeno-associated virus

Publications (1)

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CN114402066A true CN114402066A (zh) 2022-04-26

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US (2) US10801042B1 (https=)
EP (1) EP3999633A4 (https=)
JP (1) JP2022541238A (https=)
KR (1) KR20230039592A (https=)
CN (1) CN114402066A (https=)
AU (1) AU2020315477A1 (https=)
WO (1) WO2021011941A1 (https=)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN117683797A (zh) * 2023-12-04 2024-03-12 广州派真生物技术有限公司 一种用于重组腺相关病毒包装的质粒系统及其应用

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US10801042B1 (en) * 2019-07-15 2020-10-13 Vigene Biosciences, Inc. Use of ion concentrations to increase the packaging efficiency of recombinant adeno-associated virus
WO2022109241A1 (en) * 2020-11-20 2022-05-27 University Of Florida Research Foundation, Incorporated Improved aav production using potassium chloride
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117683797A (zh) * 2023-12-04 2024-03-12 广州派真生物技术有限公司 一种用于重组腺相关病毒包装的质粒系统及其应用

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KR20230039592A (ko) 2023-03-21
JP2022541238A (ja) 2022-09-22
EP3999633A4 (en) 2023-08-16
WO2021011941A1 (en) 2021-01-21
US20220282277A1 (en) 2022-09-08
EP3999633A1 (en) 2022-05-25
AU2020315477A1 (en) 2022-02-17
US10801042B1 (en) 2020-10-13

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