CN114384020B - Large-field microscopic imaging method - Google Patents
Large-field microscopic imaging method Download PDFInfo
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- CN114384020B CN114384020B CN202210066313.1A CN202210066313A CN114384020B CN 114384020 B CN114384020 B CN 114384020B CN 202210066313 A CN202210066313 A CN 202210066313A CN 114384020 B CN114384020 B CN 114384020B
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- 238000003384 imaging method Methods 0.000 title claims abstract description 96
- 230000010287 polarization Effects 0.000 claims abstract description 34
- 238000000034 method Methods 0.000 claims description 8
- 230000003287 optical effect Effects 0.000 claims description 8
- 238000000386 microscopy Methods 0.000 claims 2
- 230000000007 visual effect Effects 0.000 abstract 1
- 238000005286 illumination Methods 0.000 description 11
- 238000010586 diagram Methods 0.000 description 6
- 230000005540 biological transmission Effects 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
Abstract
The invention relates to a large-field microscopic imaging method, which uses a microscopic imaging system, wherein the system comprises a light source component, a light path component and an imaging device; the light path component comprises a first polaroid, an object carrying platform, an objective lens, a polarization beam splitter, a second polaroid and a cylindrical lens; one side of the first polaroid is an S-polarized part capable of transmitting S-polarized light, and the other side of the first polaroid is a P-polarized part capable of transmitting P-polarized light, and the first polaroid is arranged between the light source assembly and the object carrying platform and is positioned on the light path of the light emitted by the light source assembly; the objective lens is arranged on the light path of the light transmitted by the first polaroid; the imaging device comprises a first imaging device and a second imaging device, and a cylindrical lens and a second polaroid which are positioned on corresponding light paths are arranged among the first imaging device, the second imaging device and the polarization beam splitter. The invention reduces the hardware cost of the equipment and can realize microscopic imaging with large visual field by using a low-pixel detector.
Description
Technical Field
The invention relates to the field of microscopic imaging, in particular to a large-field microscopic imaging method.
Background
As shown in figure 8, the light source generates uniform illumination light through the collimation device, the uniform illumination light irradiates the sample on the platform, the light transmitted through the sample is collected by the lens, and the generated parallel light is focused on the detector, namely the imaging device, by the cylindrical lens, so as to obtain the sample image.
For some applications, it is desirable to image a large sample and to do so in as short a time as possible, typically by elevating the imaging field of view of the imaging system, with the exposure time and platform movement speed unchanged. The larger imaging field of view requires the use of a larger objective lens and a higher pixel detector with the resolution being maintained, and with the increasing imaging field of view, the pixel value of the detector is required to be increased, but the higher pixel detector is expensive, and the detector manufacturing difficulty and cost index increase beyond a certain value.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a large-field microscopic imaging method and a light splitting method.
The technical scheme adopted for solving the technical problems is as follows: a microscopic imaging method of large field uses a microscopic imaging system to carry out microscopic imaging, wherein the microscopic imaging system comprises a light source assembly, a light path assembly and an imaging device, and the light source assembly is used for emitting light; the optical path component comprises a first polaroid, an object carrying platform for placing a sample, an objective lens, a polarization beam splitter, a second polaroid and a cylindrical lens; one side of the first polaroid is an S-polarized part capable of transmitting S-polarized light, and the other side of the first polaroid is a P-polarized part capable of transmitting P-polarized light, and the first polaroid is arranged between the light source assembly and the object carrying platform and is positioned on the light path of the light emitted by the light source assembly; the objective lens is arranged on the light path of the light transmitted by the first polaroid; the imaging device comprises a first imaging device and a second imaging device, wherein the first imaging device is arranged on a light path of light transmitted by the polarization beam-splitting device, and the second imaging device is arranged on a light path of light reflected by the polarization beam-splitting device; a cylindrical lens and a second polaroid which are positioned on the corresponding light path are arranged between the first imaging device and the polarization beam splitter and between the second imaging device and the polarization beam splitter, and the second polaroid is arranged adjacent to the polarization beam splitter; s1, performing microscopic imaging by using a first imaging device and a second imaging device in a microscopic imaging system; s2, splicing and combining the images formed by the first imaging device and the second imaging device.
Preferably, the first imaging device is disposed corresponding to the P-polarized portion, and the second imaging device is disposed corresponding to the S-polarized portion.
Preferably, the objective lens is installed on a light path between the first polaroid and the carrying platform, a light splitting sheet is arranged between the objective lens and the first polaroid, the light splitting sheet reflects light transmitted by the first polaroid and transmits parallel light collected and generated by the objective lens, and the carrying platform is an opaque platform.
Preferably, the light-splitting sheet is disposed on an optical path of light transmitted by the first polarizing sheet at an angle of 45 degrees.
Preferably, the S-polarization portion and the P-polarization portion equally divide the first polarizing plate.
Preferably, the polarization beam splitter is a polarizing plate beam splitter.
Preferably, the first polarizer, the light source assembly, the object carrying platform and the objective lens are arranged in parallel, and the object carrying platform is a light-permeable platform.
The invention has the beneficial effects that: the invention reduces the hardware cost of the equipment, and can use two imaging devices with half pixels under the same imaging field of view requirement, thereby reducing the price of the imaging devices. Under the condition of unchanged resolution, the low-pixel detector can be used for realizing microscopic imaging of a large field, the problem that the high-pixel detector is needed to be used for the large field is solved, the technical bottleneck is broken through to a certain extent, and the dependence on foreign chips is reduced.
Drawings
FIG. 1 is a schematic view of a first optical path principle structure according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a second optical path principle structure according to an embodiment of the present invention;
FIG. 3 is a schematic view showing the transmission of a first polarizer according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a polarization beam splitter according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of an image of a first imaging device according to an embodiment of the invention;
FIG. 6 is a schematic diagram of an image of a second imaging device according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of a complete image of a sample according to an embodiment of the present invention;
FIG. 8 is a schematic diagram of the principle structure of a prior microscopic imaging light path;
part names and serial numbers in the figure: 1-a light source component 2-a first polaroid 20-a S polaroid 21-a P polaroid 3-an object carrying platform 30-a sample 4-an objective lens 40-a light splitting sheet 5-a polarized light splitting device 6-a second polaroid 7-a barrel lens 8-a first imaging device 9-a second imaging device.
Detailed Description
For the purpose of illustrating more clearly the objects, technical solutions and advantages of embodiments of the present invention, the present invention will be further described with reference to the following examples, and it is apparent that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be made by a person skilled in the art without any inventive effort, are intended to be within the scope of the present invention, based on the embodiments of the present invention.
In the present invention, as shown in fig. 1 to 7, a large-field microscopic imaging method, microscopic imaging is performed using a microscopic imaging system including a light source assembly 1, a light path assembly, and an imaging device, the light source assembly 1 being a light source assembly for emitting light, including a light source and a collimation system, generating uniform illumination light; the optical path component comprises a first polaroid 2, an object carrying platform 3 for placing a sample 30, an objective lens 4, a polarization beam splitter 5, a second polaroid 6 and a barrel lens 7; one side of the first polarizer 2 is an S-polarization part 20 which can transmit S-polarized light, and the other side of the first polarizer 2 is a P-polarization part 21 which can transmit P-polarized light, the first polarizer 2 is arranged between the light source assembly 1 and the carrying platform 3 and is positioned on the light path of the light emitted by the light source assembly 1, the first polarizer 2 selectively passes the light emitted by the light source assembly 1, and the S-polarization part 20 and the P-polarization part 21 are preferably used for equally dividing the first polarizer 2, for example, the left half of the first polarizer 2 is the S-polarization part 20, and the right half is the P-polarization part 21; the objective 4 is installed on the light path of the light transmitted by the first polarizer 2, at this time, the objective 4 is installed on the light path in parallel with the first polarizer 2 or in an included angle manner, that is, the first polarizer 2, the light source component 1, the carrying platform 3 and the objective 4 are arranged in parallel, the carrying platform 3 is a light-transmitting platform, so that the uniform illumination light irradiates the sample 30 placed on the carrying platform 3 after passing through the first polarizer 2, the light transmitted through the sample 30 is collected by the objective 4 to generate parallel light to be transmitted to the polarization beam splitter 5 to be split, and the polarization beam splitter 5 selects a polarization flat beam splitter; the objective lens 4 is installed on the optical paths of the first polarizer 2 and the carrying platform 3, so that the light source component 1 generates uniform illumination light, the uniform illumination light passes through the first polarizer 2, the first polarizer 2 is divided into a left part and a right part, the left part transmits the S polarized light to form an S polarized part 20, the right part transmits the P polarized light to form a P polarized part 21, as shown in fig. 3, the uniform illumination light of the first polarizer 2 irradiates the sample 30 on the carrying platform 3, the S polarized light irradiates the left half of the sample 30, the P polarized light irradiates the right half of the sample 30, the light transmitted through the sample 30 is collected by the objective lens 4, the generated parallel light is transmitted through the polarization light splitting device 5, the P polarized light is reflected, the polarization light splitting principle of the polarization light splitting device 5 is as shown in fig. 4, the transmitted light is filtered for the second time by the second polarizer 6 on the transmission path, and then focused on the first imaging device 8, namely the detector through the barrel lens 7, and the image one image of the right half of the sample 30 is obtained as shown in fig. 5; the reflected light is filtered twice by the second polaroid 6 on the reflected light path and then focused on the second imaging device 9 through the cylindrical lens 7 to obtain a left half sample 30 image II, as shown in fig. 6, and finally the two images are spliced into one image to obtain a required image, as shown in fig. 7, the imaging device comprises a first imaging device 8 and a second imaging device 9, the first imaging device 8 is arranged on the light path of the light transmitted by the polarization beam splitter 5, the second imaging device 9 is arranged on the light path of the light reflected by the polarization beam splitter 5, the first imaging device 8 is preferably arranged corresponding to the P polarization part 21, the second imaging device 9 is arranged corresponding to the S polarization part 20, so that the P polarization light is transmitted and the S polarization light is reflected; a cylindrical lens 7 and a second polaroid 6 which are positioned on corresponding light paths are arranged between the first imaging device 8 and the polarization beam splitter 5 and between the second imaging device 9 and the polarization beam splitter 5, and the second polaroid 6 is arranged adjacent to the polarization beam splitter 5. When the objective lens 4 and the first polarizer 2 are not arranged in parallel, a light-splitting sheet 40 is arranged between the objective lens 4 and the first polarizer 2, the light-splitting sheet 40 reflects the light transmitted by the first polarizer 2 and transmits the parallel light collected by the objective lens 4, the carrier platform 3 is an opaque platform, the light source component 1 generates uniform illumination light, the uniform illumination light passes through the first polarizer 2, the first polarizer 2 is divided into a left part and a right part, the left part transmits S-polarized light, the right part transmits P-polarized light, the uniform illumination light is reflected by the light-splitting sheet (50% reflection and 50% transmission), the uniform illumination light is reflected to the rear end face of the objective lens 4, the light-splitting sheet 40 is arranged on the light path of the light transmitted by the first polarizer 2 at an angle of 45 degrees, the focused light irradiates the left half of the sample, the P-polarized light irradiates the right half of the sample, the reflected light of the sample is collected by the objective lens 4, the generated parallel light passes through the light-splitting sheet 40 to reach the polarization-splitting device 5, the P-polarized light is transmitted, the S-polarized light is reflected, the transmitted light is reflected by the second polarization light is located on the transmission light path 6, and passes through the second polarization tube 7 to the first imaging device 8, and the second imaging device 8 is obtained; the reflected light is filtered twice by the second polaroid 6 on the reflected light path and focused on the second imaging device 9 through the cylindrical lens 7, so that a second left half sample image is obtained, and the two images are spliced into one image.
Further improvements, the imaging procedure is as follows,
s1, microscopic imaging is carried out by using a first imaging device 8 and a second imaging device 9 in a microscopic imaging system, corresponding S polarized light and P polarized light are generated by a first polaroid 2, then light generated by different parts of a sample irradiated by different polarized light is imaged in a corresponding imaging device, and images of different parts of the sample are formed;
s2, splicing and combining the images formed by the first imaging device 8 and the second imaging device 9, and combining the images of different parts of the sample to form a complete image of the sample.
It will be understood that modifications and variations will be apparent to those skilled in the art from the foregoing description, and it is intended that all such modifications and variations be included within the scope of the following claims.
Claims (7)
1. A large-field microscopic imaging method is characterized in that a microscopic imaging system is used for microscopic imaging, the microscopic imaging system comprises a light source assembly, a light path assembly and an imaging device, and the light source assembly is used for emitting light; the optical path component comprises a first polaroid, an object carrying platform for placing a sample, an objective lens, a polarization beam splitter, a second polaroid and a cylindrical lens; one side of the first polaroid is an S-polarized part capable of transmitting S-polarized light, and the other side of the first polaroid is a P-polarized part capable of transmitting P-polarized light, and the first polaroid is arranged between the light source assembly and the object carrying platform and is positioned on the light path of the light emitted by the light source assembly; the objective lens is arranged on the light path of the light transmitted by the first polaroid; the imaging device comprises a first imaging device and a second imaging device, wherein the first imaging device is arranged on a light path of light transmitted by the polarization beam-splitting device, and the second imaging device is arranged on a light path of light reflected by the polarization beam-splitting device; a cylindrical lens and a second polaroid which are positioned on the corresponding light path are arranged between the first imaging device and the polarization beam splitter and between the second imaging device and the polarization beam splitter, and the second polaroid is arranged adjacent to the polarization beam splitter; s1, performing microscopic imaging by using a first imaging device and a second imaging device in a microscopic imaging system; s2, splicing and combining the images formed by the first imaging device and the second imaging device.
2. The method of large field microscopy imaging of claim 1, wherein the first imaging means is configured to correspond to a P-polarized portion and the second imaging means is configured to correspond to an S-polarized portion.
3. The method of claim 1, wherein the objective lens is mounted on an optical path between the first polarizer and the object carrying platform, a light splitting sheet is disposed between the objective lens and the first polarizer, the light splitting sheet reflects light transmitted by the first polarizer and transmits parallel light collected by the objective lens, and the object carrying platform is an opaque platform.
4. The method of claim 3, wherein the light-splitting sheet is disposed at an angle of 45 degrees on the light path of the light transmitted by the first polarizing sheet.
5. The method of large field microscopy imaging of claim 1, wherein the S polarizing portion and the P polarizing portion bisect the first polarizer.
6. The method of claim 1, wherein the polarizing beamsplitter is a polarizing plate beamsplitter.
7. The method of claim 1, wherein the first polarizer, the light source assembly, the object carrying platform and the objective lens are arranged in parallel, and the object carrying platform is a light-permeable platform.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210066313.1A CN114384020B (en) | 2022-01-20 | 2022-01-20 | Large-field microscopic imaging method |
| PCT/CN2022/086209 WO2023137882A1 (en) | 2022-01-20 | 2022-04-12 | Large field-of-view microscopic imaging method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210066313.1A CN114384020B (en) | 2022-01-20 | 2022-01-20 | Large-field microscopic imaging method |
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| Publication Number | Publication Date |
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| CN114384020A CN114384020A (en) | 2022-04-22 |
| CN114384020B true CN114384020B (en) | 2024-01-30 |
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| CN202210066313.1A Active CN114384020B (en) | 2022-01-20 | 2022-01-20 | Large-field microscopic imaging method |
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| WO (1) | WO2023137882A1 (en) |
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| JP5068422B2 (en) * | 2004-10-05 | 2012-11-07 | 株式会社日立ハイテクノロジーズ | Microstructure observation method and defect inspection apparatus |
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2022
- 2022-01-20 CN CN202210066313.1A patent/CN114384020B/en active Active
- 2022-04-12 WO PCT/CN2022/086209 patent/WO2023137882A1/en unknown
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101871815A (en) * | 2009-04-24 | 2010-10-27 | 中国科学院西安光学精密机械研究所 | Programmable polarization hyperspectral imager based on aperture segmentation and acoustic-optic tunable filter |
| CN101907776A (en) * | 2009-06-04 | 2010-12-08 | 索尼公司 | The frame unit of video display devices and video display devices |
| CN103403528A (en) * | 2011-02-28 | 2013-11-20 | 国立大学法人香川大学 | Optical characteristics measuring apparatus, and optical characteristics measuring method |
| CN102944937A (en) * | 2012-11-27 | 2013-02-27 | 北京理工大学 | Sub-aperture polarization imaging system |
| CN103134756A (en) * | 2013-02-28 | 2013-06-05 | 华南师范大学 | Polarization state tomography micro-imaging device and method thereof |
| CN104792415A (en) * | 2015-04-10 | 2015-07-22 | 中国科学院光电研究院 | Complete-polarization high-spectral imaging unit |
| CN108414088A (en) * | 2017-02-10 | 2018-08-17 | 南京理工大学 | A kind of micro- polarization imaging device of EO-1 hyperion and method |
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| CN113218635A (en) * | 2021-04-30 | 2021-08-06 | 重庆大学 | Non-contact vector polarization light field test system |
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| WO2023137882A1 (en) | 2023-07-27 |
| CN114384020A (en) | 2022-04-22 |
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