CN114383915B - Portable corn pollen on-site collecting and dyeing device - Google Patents

Portable corn pollen on-site collecting and dyeing device Download PDF

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Publication number
CN114383915B
CN114383915B CN202111411261.9A CN202111411261A CN114383915B CN 114383915 B CN114383915 B CN 114383915B CN 202111411261 A CN202111411261 A CN 202111411261A CN 114383915 B CN114383915 B CN 114383915B
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China
Prior art keywords
glass
dyeing
glass slide
cavity
cover glass
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Active
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CN202111411261.9A
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Chinese (zh)
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CN114383915A (en
Inventor
王娜
刘琪
尚文鑫
徐婷
赵雪峰
王旭清
孟维伟
徐杰
南镇武
代红翠
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Xuzhou Xiyi Kangcheng Food Inspection and Testing Research Institute Co Ltd
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Xuzhou Xiyi Kangcheng Food Inspection and Testing Research Institute Co Ltd
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Priority to CN202111411261.9A priority Critical patent/CN114383915B/en
Publication of CN114383915A publication Critical patent/CN114383915A/en
Application granted granted Critical
Publication of CN114383915B publication Critical patent/CN114383915B/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

A portable corn pollen on-site collecting and dyeing device is used for conveniently collecting and dyeing collected corn pollen. The inner cavity of the shell comprises a dyeing cavity, a mounting cavity and a storage cavity, and the glass slide box and the cover glass slide box are positioned in the dyeing cavity and arranged in front of and behind the dyeing cavity; the adsorption transfer units are arranged in the mounting cavity, correspond to the glass slide boxes and the cover glass boxes one by one, and are used for taking out the glass slides in the glass slide boxes and the cover glass slides in the cover glass boxes and placing the glass slides and the cover glass boxes between the glass slide boxes and the cover glass boxes; the bearing unit comprises a supporting plate which is arranged in a vertical moving way, and the distance of each downward movement of the supporting plate is the thickness of a glass slide or a cover glass; the dyeing unit comprises a dripper positioned on the upper part of the dyeing cavity and above the supporting plate, a water pump arranged in the mounting cavity and a dropper arranged between the water pump and the dripper. The invention can conveniently collect and dye pollen.

Description

Portable corn pollen on-site collecting and dyeing device
Technical Field
The invention relates to the technical field of pollen activity detection, in particular to a portable corn pollen on-site collecting and dyeing device.
Background
The pollen with vitality has stronger respiration effect, and NADH2 or NADPH2 generated by the pollen can reduce colorless TTC (2, 3, 5-triphenyltetrazolium chloride) into red TTF (triphenylmethyl) to color the pollen, the non-vitality pollen has weaker respiration effect, and the color change of the TTC is not obvious, so that the vitality of the pollen can be judged according to the color change of the pollen after the TTC is absorbed. In the prior art, when corn pollen is detected for activity, the corn pollen is firstly obtained in a field, then brought back to a laboratory for dyeing, and then observed on a microscope. However, pollen is easy to be inactivated in the transportation process, so that the detection accuracy is affected. In addition, pollen is easy to be agglomerated in the process of transporting pollen, and experimental research is inconvenient. In addition, it takes much time to formulate the stain before pollen activity is measured; when the pollen is dyed, ensuring the uniformity of the pollen on the glass slide is also a technical activity.
Disclosure of Invention
The invention aims to provide a portable corn pollen on-site collecting and dyeing device which is used for conveniently collecting and dyeing collected corn pollen.
The technical scheme adopted for solving the technical problems is as follows: the utility model provides a dyeing apparatus is collected on spot to portable maize pollen, which characterized in that it includes:
the dyeing device comprises a shell, wherein the inner cavity of the shell comprises a dyeing cavity, a mounting cavity and a storage cavity which are mutually independent, the dyeing cavity is formed in a left-right mode, and the mounting cavity and the storage cavity are arranged up and down and are positioned between the dyeing cavities;
the device comprises a glass slide box and a cover glass box, wherein the glass slide box and the cover glass box are positioned in a dyeing cavity and are arranged in front of and behind each other, glass slides which are placed in an up-down stacking manner are placed in the glass slide box, and cover glass slides which are placed in an up-down stacking manner are placed in the cover glass box;
the adsorption transfer units are arranged in the mounting cavity, correspond to the glass slide boxes and the cover glass boxes one by one, and are used for taking out the glass slides in the glass slide boxes and the cover glass slides in the cover glass boxes and placing the glass slides and the cover glass boxes between the glass slide boxes and the cover glass boxes;
the bearing unit is arranged between the glass slide box and the cover glass box and comprises a supporting plate which is arranged in a vertically moving way, and the distance of each downward movement of the supporting plate is the thickness of the glass slide or the cover glass;
the dyeing unit comprises a dripper positioned on the upper part of the dyeing cavity and above the supporting plate, a water pump arranged in the installation cavity, and a dropper arranged between the water pump and the dripper, wherein the water pump pumps the dyeing agent and adds the dyeing agent into the dripper to dye.
Further, a pair of vertically arranged first partition plates and a second partition plate arranged between the first partition plates are arranged in the inner cavity of the shell, a dyeing cavity is formed between the first partition plates and the shell, a mounting cavity is formed between the second partition plates and the top of the shell, and a storage cavity is formed between the second partition plates and the bottom of the shell.
Further, a bottom plate is arranged in the inner cavity of the glass slide box, a spring is arranged between the bottom plate and the bottom of the glass slide box, a glass slide is placed above the bottom plate, a notch is arranged at the top of the glass slide box, an outlet communicated with the notch is arranged on the side wall of the glass slide box, and the glass slide in the glass slide box is moved out through the outlet; the structure of the cover glass box is the same as that of the glass box.
Further, the adsorption transfer unit comprises a sucker arm arranged on the side wall of the shell in a sliding manner, a sucker fixed at the bottom of the sucker arm and a first driving mechanism for driving the sucker arm to move back and forth, wherein in the process of moving the sucker arm back and forth, the moving track of the sucker arm comprises two sections of straight lines which are arranged in parallel up and down and an inclined line which is arranged between the two straight lines.
Further, a pair of protrusions are arranged at the mounting end of the sucker arm, a sliding block is fixed on the protrusions, two guide grooves are formed in the side wall of the shell, each guide groove comprises a first horizontal groove, a second horizontal groove and an inclined groove arranged between the first horizontal groove and the second horizontal groove, and the sliding block is arranged in each guide groove in a sliding mode.
Further, the first driving mechanism comprises a swing motor fixed in the mounting cavity, a turntable fixed at the output end of the swing motor and a swing arm fixed on the turntable, the swing arm is of a L-shaped structure, the lower end of the swing arm is fixedly connected with the turntable, the upper end of the swing arm is in a horizontal state, an elongated slot is arranged at the end part of the upper end of the swing arm, a push rod is fixed at the top of the sucker frame, and the upper end of the push rod extends upwards after passing through the elongated slot.
Further, the bearing unit comprises a supporting plate, a screw nut fixedly connected with the supporting plate, a screw matched with the screw nut and a lifting motor for driving the screw to rotate.
Further, a liquid inlet hole is arranged at the top of the dripper, symmetrically arranged dye flow passages are arranged in the dripper, the dye flow passages are communicated with the liquid inlet hole, and the dye flow passages and the liquid inlet hole form a herringbone shape.
The beneficial effects of the invention are as follows: the portable corn pollen on-site collecting and dyeing device provided by the invention can store the glass slide and the cover glass, can acquire the glass slide and the cover glass one by one, and can finish collecting and dyeing the corn pollen after the glass slide is acquired, pollen is placed, a dyeing agent is dripped and the cover glass is covered. Compared with the prior art, the corn pollen collecting and dyeing device can realize rapid collection and dyeing of corn pollen.
Drawings
FIG. 1 is a front view of the present invention;
FIG. 2 is a schematic view (front view) of the internal structure of the present invention;
FIG. 3 is a schematic view of the internal structure of the present invention (top view);
FIG. 4 is a cross-sectional view of a slide cassette;
FIG. 5 is a rear view of the slide cassette;
FIG. 6 is a schematic diagram of the structure of the adsorption transfer unit;
FIG. 7 is a schematic view of the assembly of the suction cup arm with the housing;
FIG. 8 is a top view of the present invention;
FIG. 9 is a schematic view of the suction cup of the suction transfer unit moving over the pallet;
FIG. 10 is a schematic diagram of a process for placing pollen, stain and coverslip on a slide;
FIG. 11 is a schematic view of a slide cassette, a cover slip on the cover slip cassette, and a cover slip removal process;
FIG. 12 is a schematic view of the assembly of a dripper and a dropper;
FIG. 13 is a schematic view of a structure of a carrying unit;
FIG. 14 is a schematic view of a carrier unit carrying a slide;
FIG. 15 is a schematic view of the assembly of the slider and swing arm (front view);
in the figure: 1 casing, 11 landing leg, 12 side door, 13 handle, 14 first baffle, 141 through hole, 15 second baffle, 16 guide slot, 161 first horizontal slot, 162 inclined slot, 163 third horizontal slot, 17 pollen inlet, 18 travel switch, 2-chip box, 21 bottom plate, 22 spring, 23 notch, 24 outlet, 2' cover glass box, 3 slide, 31 pollen, 32 colorant, 33 cover glass, 4 sucking disc arm, 41 protrusion, 42 slide block, 43 ejector pin, 44 sucking disc, 5 swing arm, 51 turntable, 52 swing motor, 53 elongated slot, 6 supporting plate, 61 supporting plate handle, 62 screw nut, 63 screw, 64 bracket, 65 lifting motor, 7 dropper, 71 dropper, 711 liquid inlet, 712 partition plate, 713 colorant runner, 72 water pump.
Detailed Description
As shown in fig. 1 to 15, the present invention mainly includes a housing 1, a slide cassette 2, a cover glass cassette 2', an adsorption transfer unit, a carrying unit, and a staining unit, and is described in detail below with reference to the accompanying drawings.
As shown in fig. 1 to 3, the housing 1 is a rectangular parallelepiped plastic member, and the housing is hollow. Three supporting legs 11 are arranged at the bottom of the shell to support the shell. The front wall and the rear wall of the shell are all open, and side doors 12 are arranged on the front wall and the rear wall of the shell so as to block the inner cavity of the shell. A handle 13 is arranged at the top of the shell so as to grasp the shell, a pair of first partition plates 14 which are arranged left and right are arranged in the inner cavity of the shell, and a second partition plate 15 which is horizontally arranged is arranged between the upper parts of the two first partition plates. A through hole 141 is provided in the middle of the first partition plate, and as shown in fig. 8, a pair of pollen inlets 17 are provided at the top of the housing, and when in use, corn pollen is added into the housing through the pollen inlets. The first and second partition boards divide the inner cavity of the shell into four parts, wherein the part between the first partition board and the left and right side walls of the shell is a dyeing cavity, the part between the second partition board and the top of the shell is an installation cavity, and the part between the second partition board and the bottom of the shell is a storage cavity. Unused slide cassettes and cover cassettes are stored in the storage chamber.
The slide glass box 2 and the cover glass box 2' which are arranged front and back are arranged in the dyeing cavity, as shown in fig. 4 and 5, the slide glass box 2 is a cuboid-shaped plastic piece, the inside of the slide glass box is hollow, a rectangular bottom plate 21 is arranged in the inner cavity of the slide glass box, a spring 22 is arranged between the bottom plate and the bottom of the slide glass box, the bottom plate is at the highest position under the action of the spring, and a slide glass 3 is placed between the bottom plate and the top of the slide glass box. The top of the slide box is provided with a notch 23, the upper part of the rear side wall of the slide box is provided with an outlet 24 communicated with the notch, the outlet is long-strip-shaped, and the slide glass passes through the outlet to move out of the slide box. The structure and the shape of the cover glass box 2' are the same as those of the cover glass box 2, and cover glass which is stacked is placed in the cover glass box. The left end and the right end of the shell are respectively provided with a glass slide box and a cover glass box, and the area between the glass slide box and the cover glass box is an area for collecting pollen and dyeing the pollen. The slides stacked on the bottom plate are closely arranged under the action of the springs, and the uppermost slide is in contact with the upper wall of the slide box and is flush with the outlet.
In order to realize the taking out of the glass slide in the glass slide box, an adsorption transfer unit is arranged between the mounting cavity and the dyeing cavity. As shown in fig. 3 and 6, the suction transfer unit includes a suction cup arm 4, a suction cup 44, and a first driving mechanism for driving the suction cup arm to slide forward and backward, the suction cup arm 4 is a substantially rectangular rod, protrusions 41 are fixed to the top and bottom of the mounting end of the suction cup arm, and a slider 42 is fixed to the protrusions. As shown in fig. 7, guide grooves 16 are formed in the left side wall and the right side wall of the housing, and the number of guide grooves in the left side wall of the housing is two, and the number of guide grooves in the right side wall of the housing is also two. The guide groove includes a first horizontal groove 161 and a second horizontal groove 163 which are disposed up and down, and an inclined groove 162 which is disposed between the first horizontal groove and the second horizontal groove, and the first horizontal groove, the inclined groove and the second horizontal groove are disposed in sequence and connected end to end. The sliding blocks are in one-to-one correspondence with the guide grooves, and the sliding blocks are in sliding connection with the corresponding guide grooves. As shown in fig. 15, a push rod 43 is fixed on the top of the free end of the slide block, and a sucker 44 is arranged at the bottom of the free end of the slide block, and the sucker adsorbs a glass slide or a cover glass by the principle of negative pressure adsorption. The first driving mechanism comprises a swing motor 52 fixed in the mounting cavity, a rotary table 51 fixed at the output end of the swing motor, and a swing arm 5 fixed on the rotary table, wherein the swing arm is of a L-shaped structure, the lower end of the swing arm is fixedly connected with the rotary table, the upper end of the swing arm is in a horizontal state, the end part of the upper end of the swing arm is provided with a long groove 53, and the upper end of the ejector rod extends upwards after passing through the long groove. As shown in fig. 9, when the swing motor works, the swing arm can be driven to swing, and when the swing arm swings, the sucker arm is driven to move forwards and backwards. When the sucker arm moves forwards and backwards, the sucker arm is driven to move forwards and backwards. And when the slide block is arranged in the first horizontal groove, the sucker adsorbs the glass slide. When the slide block passes through the inclined groove and enters the second horizontal groove, the slide glass moves out of the slide glass box along with the sucker and then moves downwards for one end distance. The first driving mechanism is provided with four glass slide boxes and two glass cover slide boxes respectively and is used for transferring glass slides in the glass slide boxes or glass covers in the glass cover slide boxes into the dyeing area.
The dyeing area is provided with a bearing unit, and the bearing unit is used for bearing the glass slide, as shown in fig. 13 and 14, and comprises a rectangular bracket 64 fixed in the storage cavity, a screw 63 rotatably arranged on the inner side of the bracket, a lifting motor 65 for driving the screw to rotate, a screw nut 62 matched with the screw, a supporting plate handle 61 fixedly connected with the screw, and a supporting plate 6 fixed on the supporting plate handle, wherein the supporting plate is a rectangular plastic plate. The lifting motor is fixed at the bottom of the support, and when the lifting motor works, the screw rod is driven to rotate, so that the screw rod nut is driven to move up and down, and the supporting plate is lifted. The lifting motor, the screw rod and the screw rod nut form a second driving mechanism. For each action of the lifting motor, the downward moving distance of the supporting plate is the thickness of one glass slide or cover glass. Before use, the supporting plate is arranged at the highest point, and the upper surface of the supporting plate is flush with the lower edge of the second horizontal groove. When the slide block moves into the second horizontal groove, the lower surface of the glass slide or the cover glass on the sucker is flush with the supporting plate, namely, the adsorption transfer unit acts once, the glass slide is taken out of the glass slide box or the cover glass is taken out of the cover glass box, the glass slide or the cover glass is placed on the supporting plate, and then the supporting plate is driven to move downwards by the thickness of one glass slide or the cover glass under the action of the second driving mechanism, wherein the thickness of the glass slide is equal to that of the cover glass. As shown in fig. 11, after a slide 3 in the slide cassette 2 is removed and placed on the tray, a cover glass 33 is then removed from the cover glass cassette 2' for placement on the slide.
When in use, as shown in fig. 10, a glass slide is taken out of the glass slide box and placed on the supporting plate, then collected corn pollen is added into the pollen inlet, and the pollen is naturally separated and scattered on the glass slide under the action of air in the falling process of the corn pollen. The staining agent 32 may then be added dropwise to the slide, and finally, the cover slip is removed from the cover slip case by the adsorption transfer unit and covered on the slide.
In order to realize the dropwise addition of the coloring agent, a dyeing unit is arranged above the supporting plate, as shown in fig. 3 and 12, the dyeing unit comprises a dropper 7, a dropper 71 connected with the first end of the dropper, and a water pump 72 connected with the second end of the dropper, the water pump pumps the coloring agent and adds the coloring agent into the dropper through the dropper, as shown in fig. 12, a liquid inlet 711 is arranged at the top of the dropper, a partition plate 712 is arranged at the lower part of the liquid inlet, two coloring agent flow channels 713 which are symmetrically arranged with the partition plate as a symmetry center are arranged on the dropper, the communication between the liquid inlet and the coloring agent flow channels is realized, and the partition plate equally divides the coloring agent entering the coloring agent flow channels. The dyeing agent flow passage and the liquid inlet hole form a herringbone shape.
A travel switch 18 is arranged in the mounting cavity, and the swing motor stops moving after the swing arm is contacted with the travel switch. And the travel switch is arranged to limit the swing range of the swing arm, and after the swing arm is contacted with the travel switch, the sucker is transferred to the upper part of the glass-carrying box or the glass-covering box.
The working process of the invention is as follows: the adsorption transfer unit acquires a glass slide from the glass slide box and places the glass slide on a supporting plate; the operator places the collected pollen on the glass slide through the pollen inlet, drops the coloring agent on the glass slide through the coloring unit, then obtains the cover glass from the cover glass box through the adsorption transfer unit, and places the cover glass on the glass slide. Every time a slide or cover glass is placed over the tray, the carrier unit is actuated once so that the tray moves down one slide or cover glass thickness.
The invention can realize the storage of the glass slide and the cover glass, can realize the acquisition of the glass slide and the cover glass one by one, and can finish the acquisition and dyeing of the corn pollen after the pollen is placed, the coloring agent is dripped and the cover glass is covered after the glass slide is acquired. Compared with the prior art, the corn pollen collecting and dyeing device can realize rapid collection and dyeing of corn pollen.

Claims (5)

1. The utility model provides a dyeing apparatus is collected on spot to portable maize pollen, which characterized in that it includes:
the dyeing device comprises a shell, wherein the inner cavity of the shell comprises a dyeing cavity, a mounting cavity and a storage cavity which are mutually independent, the dyeing cavity is formed in a left-right mode, and the mounting cavity and the storage cavity are arranged up and down and are positioned between the dyeing cavities;
the device comprises a glass slide box and a cover glass box, wherein the glass slide box and the cover glass box are positioned in a dyeing cavity and are arranged in front of and behind each other, glass slides which are placed in an up-down stacking manner are placed in the glass slide box, and cover glass slides which are placed in an up-down stacking manner are placed in the cover glass box;
the adsorption transfer units are arranged in the mounting cavity, correspond to the glass slide boxes and the cover glass boxes one by one, and are used for taking out the glass slides in the glass slide boxes and the cover glass slides in the cover glass boxes and placing the glass slides and the cover glass boxes between the glass slide boxes and the cover glass boxes; the adsorption transfer unit comprises a sucker arm, a sucker and a first driving mechanism, wherein the sucker arm is arranged on the side wall of the shell in a sliding manner, the sucker is fixed at the bottom of the sucker arm, the first driving mechanism drives the sucker arm to move forwards and backwards, and in the process of moving the sucker arm forwards and backwards, the moving track of the sucker arm comprises two straight lines which are arranged in parallel up and down, and an inclined line which is arranged between the two straight lines; the mounting end of the sucker arm is provided with a pair of protrusions which are arranged up and down, a sliding block is fixed on the protrusions, the side wall of the shell is provided with two guide grooves, each guide groove comprises a first horizontal groove, a second horizontal groove and an inclined groove arranged between the first horizontal groove and the second horizontal groove, and the sliding block is arranged in each guide groove in a sliding manner; the first driving mechanism comprises a swing motor fixed in the mounting cavity, a rotary table fixed at the output end of the swing motor, and a swing arm fixed on the rotary table, wherein the swing arm is of a L-shaped structure, the lower end of the swing arm is fixedly connected with the rotary table, the upper end of the swing arm is in a horizontal state, the end part of the upper end of the swing arm is provided with a long groove, the top of the free end of the sucker arm is fixedly provided with a push rod, and the upper end of the push rod extends upwards after passing through the long groove;
the bearing unit is arranged between the glass slide box and the cover glass box and comprises a supporting plate which is arranged in a vertically moving way, and the distance of each downward movement of the supporting plate is the thickness of the glass slide or the cover glass; the top of the shell is provided with a pair of pollen inlets which are arranged left and right, and pollen is scattered on the glass slide on the supporting plate through the pollen inlets; the adsorption transfer unit takes out the glass slide or cover glass and places the glass slide or cover glass on the supporting plate;
the dyeing unit comprises a dripper positioned on the upper part of the dyeing cavity and above the supporting plate, a water pump arranged in the installation cavity, and a dropper arranged between the water pump and the dripper, wherein the water pump pumps the dyeing agent and adds the dyeing agent into the dripper to dye.
2. The portable corn pollen on-site collection and dyeing device according to claim 1, wherein a pair of first partition plates arranged vertically and a second partition plate arranged between the first partition plates are arranged in the inner cavity of the shell, a dyeing cavity is formed between the first partition plates and the shell, a mounting cavity is formed between the second partition plates and the top of the shell, and a storage cavity is formed between the second partition plates and the bottom of the shell.
3. The portable corn pollen on-site collecting and dyeing device according to claim 1, wherein a bottom plate is arranged in the inner cavity of the glass slide box, a spring is arranged between the bottom plate and the bottom of the glass slide box, a glass slide is placed above the bottom plate, a notch is arranged at the top of the glass slide box, an outlet communicated with the notch is arranged on the side wall of the glass slide box, and the glass slide in the glass slide box is moved out through the outlet; the structure of the cover glass box is the same as that of the glass box.
4. The portable corn pollen on-site collecting and dyeing device according to claim 1, wherein the bearing unit comprises a supporting plate, a screw nut fixedly connected with the supporting plate, a screw matched with the screw nut and a lifting motor for driving the screw to rotate.
5. The portable corn pollen on-site collecting and dyeing device according to claim 1, wherein the top of the dripper is provided with a liquid inlet, the dripper is internally provided with symmetrically arranged dye flow passages, the dye flow passages are communicated with the liquid inlet, and the dye flow passages and the liquid inlet form a herringbone shape.
CN202111411261.9A 2021-11-25 2021-11-25 Portable corn pollen on-site collecting and dyeing device Active CN114383915B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111411261.9A CN114383915B (en) 2021-11-25 2021-11-25 Portable corn pollen on-site collecting and dyeing device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111411261.9A CN114383915B (en) 2021-11-25 2021-11-25 Portable corn pollen on-site collecting and dyeing device

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CN114383915A CN114383915A (en) 2022-04-22
CN114383915B true CN114383915B (en) 2023-11-21

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