CN114380474B - Excrement treating agent and method and application thereof for treating excrement containing chicken cecum core - Google Patents
Excrement treating agent and method and application thereof for treating excrement containing chicken cecum core Download PDFInfo
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- CN114380474B CN114380474B CN202111571410.8A CN202111571410A CN114380474B CN 114380474 B CN114380474 B CN 114380474B CN 202111571410 A CN202111571410 A CN 202111571410A CN 114380474 B CN114380474 B CN 114380474B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Abstract
The invention belongs to the field of animal waste treatment, and discloses a waste treatment agent, a method for treating waste containing chicken cecum cores by using the same and application of the waste treatment agent, wherein the waste treatment agent comprises the following components: sodium hypochlorite, subtilisin, cellulase and water-soluble auxiliary agent; the water-soluble auxiliary agent is one or two of polyethylene glycol or propylene glycol n-propyl ether. According to the method, excrement liquid obtained after excrement is mixed with water is treated by adopting an excrement treating agent, a caecum core in the excrement liquid mainly comprises an epithelial tissue peeled off from caecum and undigested cellulose in feed, the caecum core is hard and firm, the epithelial tissue and the undigested cellulose in the caecum core can be hydrolyzed by using subtilisin and cellulase in the excrement treating agent, the caecum core is softened, and then the ultrasonic cavitation treatment is carried out on the excrement liquid, so that the caecum core is fully crushed and the eimeria tenella oocysts in the caecum core are released.
Description
Technical Field
The invention belongs to the field of animal waste treatment, and particularly relates to a waste treatment agent, a method for treating waste containing chicken cecum cores by using the same and application of the waste treatment agent.
Background
Eimeria gallinarum is a parasitic protozoal disease caused by various species of Eimeria which parasitize in intestinal epithelial cells of chicken, and the parasitic parts and pathogenicity of different species of Eimeria gallinarum are different, while Eimeria tenella is the most pathogenic. The coccidiosis of the chicken is wide in distribution and large in harm, and is mainly characterized in that the affected chicken is emaciated, has reduced appetite, is anaemia and bloody dysentery, not only growth of the affected chicken is slowed down, weight gain and egg laying are affected, but also death can be caused seriously, and huge economic loss is brought to the chicken breeding industry.
With the popularization of food safety concept, more and more breeders use vaccines to prevent chicken coccidiosis. The preparation of the chicken coccidium vaccine requires that the coccidium oocysts are separated from the excrement of the infected chicken, and in the separation process of the chicken Eimeria tenella strain, the excrement of the chicken infected with Eimeria tenella contains cecal cores (see figure 1), and because the coccidium is mainly parasitized in chicken cecal epithelial cells, a large amount of Eimeria tenella oocysts are contained in the discharged cecal cores (see figure 2, a large amount of oocysts can be seen by smear microscopy after the cecal cores are ground to be homogeneous), and the cecal cores are firm, so that the oocysts are difficult to release and obtain. While the tissue grinding or pounding machine is used for processing the cecal cores to influence the synchronization of oocyst sporulation development and sporulation rate, the cecal cores of hard blocks are manually rubbed and crushed at present, water is added for filtration, and the process is repeated for 3 to 4 times, which is time-consuming and labor-consuming. Although the artificial rubbing does not cause adverse effect on the oocysts, the manual rubbing not only consumes manpower, reduces the separation efficiency and prolongs the separation time, but also reduces the recovery rate of the oocysts due to insufficient release.
Therefore, aiming at the problem of treatment of cecum core excrement in the separation process of chicken Eimeria tenella oocysts, the invention provides a solution, and the cecum core is made to be soft and fragile by adding a self-made excrement treatment agent, so that the separation efficiency and the oocyst recovery rate of the Eimeria tenella oocysts are improved.
Disclosure of Invention
Aiming at the problem of cecum core treatment in the separation and extraction process of chicken eimeria tenella oocysts, the invention provides a fecal treatment agent, a method for treating feces containing chicken cecum cores by using the fecal treatment agent and application of the fecal treatment agent, and the method remarkably improves the separation efficiency and the oocyst recovery rate of the eimeria tenella oocysts.
In order to achieve the purpose, the invention adopts the following technical scheme:
the excrement treating agent comprises the following components: sodium hypochlorite, subtilisin, cellulase and water-soluble auxiliary agent; the water-soluble auxiliary agent is one or two of polyethylene glycol or propylene glycol n-propyl ether.
Preferably, the fecal treatment agent for the chicken cecum core comprises the following components in parts by weight: 5-10 parts of sodium hypochlorite, 3-6 parts of subtilisin, 5-10 parts of cellulase and 10-15 parts of water-soluble auxiliary agent.
Preferably, the polyethylene glycol is at least one of polyethylene glycol 300, polyethylene glycol 1000, polyethylene glycol 2000, polyethylene glycol 4000 and polyethylene glycol 6000.
Further preferably, the polyethylene glycol is at least one of polyethylene glycol 1000 and polyethylene glycol 2000.
Sodium hypochlorite: sterilizing, inhibiting bacteria growth, maintaining feces properties, and preventing feces from producing large amount of acid due to bacteria, thereby changing feces properties.
Subtilisin: the protein between the caecum core blood hard tissue is hydrolyzed to loosen the hard tissue.
Cellulase: the cellulose in the caecum core is degraded, the attachment degree of the oocysts is reduced, and the oocysts are released from blood hard tissue.
Water-soluble auxiliary agent: the water solubility of the fecal treatment agent is enhanced, so that the sodium hypochlorite, the subtilisin and the cellulase can be contacted with the cecal core more, and the cecal core is treated.
Further preferably, the water-soluble assistant is polyethylene glycol.
Polyethylene glycol: good water solubility and good intermiscibility with other components, thereby enhancing the water solubility of the fecal treatment agent, leading the sodium hypochlorite, the subtilisin and the cellulase to be more contacted with the caecum core for treating the caecum core.
Propylene glycol n-propyl ether: the HLB value is about 12, the HLB value is suitable and extremely high in safety, and the HLB-modified liquid manure treatment agent can be used as a solvent to enhance the water solubility of the manure treatment agent.
Preferably, the faeces are faeces comprising a chicken cecal core.
The method for treating the excrement containing the chicken cecum core by using the excrement treating agent comprises the following steps of:
(1) Adding water into excrement containing the cecum core of the chicken for mixing to obtain excrement liquid, adding the excrement treating agent to treat the excrement liquid, and standing to obtain turbid liquid;
(2) And sieving the turbid solution, centrifuging and collecting precipitate to obtain the compound.
Preferably, the step (2) further comprises adding saturated saline solution into the precipitate, stirring, centrifuging, and collecting supernatant; adding water into the supernatant, centrifuging, and collecting precipitate to obtain coccidian oocysts.
Further preferably, the supernatant is an oocyst supernatant.
More preferably, the liquid-solid ratio of the saturated saline to the precipitate is (1-3): 1L/kg.
Further preferably, the volume ratio of the supernatant to the water is 1 (5-10).
Preferably, in the step (2), before the step of screening the turbid liquid, the step of subjecting the turbid liquid to ultrasonic treatment is further included; the power of ultrasonic treatment is 300-600W, the frequency of ultrasonic treatment is 20-40KHz, and the time is 10-30min.
Principle of sonication: when ultrasonic waves are spread along gaps of liquid in turbid liquid, molecules of the liquid are transmitted by ultrasonic energy and have energy, the molecules interact to generate a large number of bubbles, the bubbles form a precondition of cavitation, and when the energy is gathered to a certain degree, the bubbles are broken to decompose hard blood tissue in excrement into a homogeneous state, so that subsequent treatment is facilitated.
The invention utilizes cavitation of ultrasonic cleaning, namely countless bubbles in the turbid liquid are rapidly formed and rapidly implode, and the generated impact can fully remove blood hard mass tissues in the turbid liquid and release the coccidian oocysts in the turbid liquid.
Preferably, in the step (1), the solid-to-liquid ratio of the feces containing the chicken cecum core to water is 1: (1-1.2) kg/L.
Preferably, in the step (1), the mass ratio of the excrement treating agent to the excrement containing the chicken cecal core is 1 (100-120).
Preferably, in the step (1), the standing time is 10-30min.
Preferably, in the step (2), before the step of sieving the turbid liquid, the step of subjecting the turbid liquid to ultrasonic treatment is further included; the power of the ultrasonic wave is 300-600W, the frequency is 20-40KHz, and the time is 10-30min.
Preferably, in the step (2), the sieving is performed by using 200-400 meshes, and the rotating speed of the centrifugation is 2000-3000r/min.
The invention also provides application of the excrement treating agent in treating animal excrement.
Preferably, the animal manure is chicken manure.
Advantageous effects
(1) According to the method, the fecal treatment agent is added into the fecal solution, the caecum core in the fecal solution is mainly caecum bleeding, mucosal epithelial tissue shedding and undigested cellulose in the feed, so that the caecum core is hard and firm, the subtilisin and the cellulase in the fecal treatment agent can hydrolyze the epithelial tissue and the undigested cellulose in the caecum core to soften the caecum core, and then the fecal solution is subjected to ultrasonic smashing treatment to fully smash the caecum core and release the eimeria tenella oocysts in the caecum core, so that the oocyst recovery rate of the eimeria tenella oocysts is improved.
(2) Compared with the prior art which needs repeated crushing and filtering for three or four times, the method for adding the excrement treating agent only needs to add water for filtering for one to two times, so that the separation efficiency can be obviously improved by more than 20 percent, the link of manual crushing is reduced, the time required by separation is shortened, the recovery rate of oocysts is improved to more than 98 percent, and the preparation cost of the large-scale coccidian oocysts is reduced.
(3) The fecal treatment agent has the advantages of reasonable formula, low cost, convenient use, simple and convenient ultrasonic fragmentation operation, obvious effect and no adverse effect and damage to oocysts.
Drawings
FIG. 1 is a graph of excretions containing cecal cores from Eimeria tenella infected chickens of the present invention;
FIG. 2 is a smear of oocyst microscopy images of cecal cores of the present invention ground to homogeneity;
FIG. 3 is a cecum core diagram of a chicken;
FIG. 4 is a endoscopic view of a cecal core doctor blade according to example 1 of the present invention;
FIG. 5 is a microscopic view of the residue in example 1 of the present invention;
FIG. 6 is a residue microscopic view of example 2 of the present invention;
FIG. 7 is a residue microscopic view of example 3 of the present invention;
FIG. 8 is oocyst examination of coccidia of example 3 of the present invention;
FIG. 9 is the coccidia oocyst sporulation of example 3 of the present invention;
FIG. 10 is a microscopic image of the residue of comparative example 1 of the present invention.
FIG. 11 is a microscopic image of the residue of comparative example 2 of the present invention.
Detailed Description
In order to make the technical solutions of the present invention more clearly apparent to those skilled in the art, the following examples are given for illustration. It should be noted that the following examples are not intended to limit the scope of the claimed invention.
The starting materials, reagents or apparatuses used in the following examples are conventionally commercially available or can be obtained by conventionally known methods, unless otherwise specified.
Example 1
The fecal treatment agent of the embodiment comprises the following components in parts by weight: 5 parts of sodium hypochlorite, 3 parts of subtilisin, 5 parts of cellulase and 10 parts of polyethylene glycol 2000.
The fecal treatment agent for the cecum core of the chicken of the embodiment is prepared by mixing 5 parts of sodium hypochlorite, 3 parts of subtilisin, 5 parts of cellulase and 10 parts of polyethylene glycol.
The method for treating the excrement containing the chicken cecal cores comprises the following steps:
(1) Adding 120L of water into 100kg of feces containing chicken cecum core, mixing to obtain feces liquid, adding 1kg of feces treatment agent (sodium hypochlorite, subtilisin, cellulase and polyethylene glycol 2000), stirring, and standing for 30min to obtain turbid liquid;
(2) Putting the turbid liquid into an ultrasonic machine, carrying out ultrasonic cavitation treatment for 20 minutes under the ultrasonic power of 500W and the frequency of 40KHz, then sieving by a 300-mesh sieve, centrifuging the turbid liquid subjected to ultrasonic cavitation treatment for 1 minute at the rotating speed of 2000r/min, and collecting centrifugal precipitates;
(3) Adding saturated salt water into the precipitate according to a liquid-solid ratio of 2:1kg/L, stirring, centrifuging at a rotating speed of 3000r/min for 1 min, and collecting supernatant;
(4) Water and supernatant (oocyst floating liquid) were mixed at a volume ratio of 10:1, mixing, centrifuging for 2 minutes at the rotating speed of 2500r/min, and collecting the centrifugal precipitate to obtain the coccidian oocysts.
The coccidian oocysts obtained in example 1 were counted. FIG. 4 is a microscopic view of the cecal core scraper of example 1; FIG. 5 is a microscopic picture of the residue of example 1 of the present invention, with some amount of oocysts remaining in the fecal residue.
Example 2
The fecal treatment agent of the embodiment comprises the following components in parts by weight: 5 parts of sodium hypochlorite, 3 parts of subtilisin, 5 parts of cellulase and 10 parts of polyethylene glycol 2000.
The method for treating the excrement containing the chicken cecal cores comprises the following steps:
(1) Adding 120L of water into 100kg of feces containing cecum core of chicken, mixing to obtain feces liquid, adding 1kg of feces treatment agent (sodium hypochlorite, subtilisin, cellulase and polyethylene glycol 2000), stirring, and standing for 30min to obtain turbid liquid;
(2) Sieving the turbid solution with a 300-mesh sieve, then centrifuging the turbid solution for 1 minute at the rotating speed of 2000r/min, and collecting precipitates;
(3) Adding saturated salt solution into the precipitate according to the liquid-solid ratio of 2:1kg/L, stirring, centrifuging at the rotation speed of 3000r/min for 1 min, and collecting oocyst floating liquid;
(4) Water and an oocyst floating solution are mixed according to the volume ratio of 10:1, mixing, centrifuging for 2 minutes at the rotating speed of 2500r/min, and collecting centrifugal precipitate to obtain the coccidian oocysts.
The resulting coccidian oocysts were counted.
Example 3
The fecal treatment agent of the embodiment comprises the following components in parts by weight: 15 parts of sodium hypochlorite, 9 parts of subtilisin, 15 parts of cellulase and 20 parts of polyethylene glycol 2000.
The method for treating the excrement containing the chicken cecal cores comprises the following steps:
(1) Adding 120L of water into 100kg of feces containing chicken cecum core, mixing to obtain feces liquid, adding 1kg of feces treatment agent (sodium hypochlorite, subtilisin, cellulase and polyethylene glycol 2000), stirring, and standing for 30min to obtain turbid liquid;
(2) Putting the turbid solution into an ultrasonic machine, carrying out ultrasonic treatment for 10-30 minutes under the ultrasonic power of 500W and the frequency of 40KHz, then sieving by a 300-mesh sieve, centrifuging for 1 minute at the rotating speed of 2000r/min, and collecting precipitates;
(3) Adding saturated salt solution into the precipitate according to the liquid-solid ratio of 2:1kg/L, stirring, centrifuging at the rotation speed of 3000r/min for 1 min, and collecting oocyst floating liquid;
(4) Water and an oocyst floating solution are mixed according to the volume ratio of 9:1, mixing, centrifuging for 2 minutes at the rotating speed of 2500r/min, and collecting the precipitate to obtain the coccidian oocysts.
The coccidian oocysts obtained in example 3 were counted. FIG. 7 is a microscopic examination of the residues in example 3 of the present invention, in example 3, the feces treatment agent wetted with polyethylene glycol is used, and sodium hypochlorite in the feces treatment agent firstly sterilizes the feces to inhibit the growth of bacteria, maintain the feces properties, and prevent the feces from producing a large amount of acid due to the bacteria, thereby changing the feces properties; and then the subtilisin and the cellulase are combined to hydrolyze protein between caecum hard tissue of the caecum core, so that the hard tissue becomes loose, the cellulose in the caecum core is degraded, the attachment degree of the oocysts is reduced, and the oocysts are released from the hard tissue of the caecum. Finally, ultrasonic cavitation treatment is combined, so that blood hard tissue in the turbid liquid is fully removed, oocysts are fully released, and the residual refractive index of the oocysts in the residues can be counted; FIG. 8 is the coccidian oocysts obtained in example 3 of the present invention after being subjected to ultrasonography, wherein the blood hard tissue in the turbid solution is sufficiently removed and the coccidian oocysts are released and collected. FIG. 9 is the coccidian oocyst sporulation of example 3 of the present invention, and the ultrasonic cavitation of the present invention does not affect the coccidian oocyst morphology.
Example 4
The fecal treatment agent of the embodiment comprises the following components in parts by weight: 15 parts of sodium hypochlorite, 9 parts of subtilisin, 15 parts of cellulase and 20 parts of polyethylene glycol 2000.
The method for treating the excrement containing the chicken cecal cores comprises the following steps:
(1) Adding 120L of water into 100kg of feces containing chicken cecum core, mixing to obtain feces liquid, adding 1kg of feces treatment agent (sodium hypochlorite, subtilisin, cellulase and polyethylene glycol 2000), stirring, and standing for 30min to obtain turbid liquid;
(2) Sieving the turbid solution with a 300-mesh sieve, centrifuging at the rotation speed of 2000r/min for 1 minute, and collecting precipitates;
(3) Adding saturated salt solution into the precipitate according to the liquid-solid ratio of 2:1kg/L, stirring, centrifuging at the rotation speed of 3000r/min for 1 min, and collecting oocyst floating liquid;
(4) Water and an oocyst floating solution are mixed according to the volume ratio of 9:1, mixing, centrifuging for 2 minutes at the rotating speed of 2500r/min, and collecting the precipitate to obtain the coccidian oocysts.
The resulting coccidial oocysts were counted.
Example 5
The difference from example 1 is that: the polyethylene glycol in the fecal treatment agent is polyethylene glycol 4000.
Example 6
The difference from example 1 is that: the water-soluble auxiliary agent is propylene glycol n-propyl ether.
Example 7
The difference from example 1 is that in step (1) of the method for treating faeces containing chicken cecal cores: 100L of water was added to 100kg of feces containing chicken cecum cores and mixed.
Comparative example 1
The method of this comparative example for treating feces containing chicken cecal cores, comprising the steps of:
(1) Adding 120L of water into 100kg of excrement containing the cecum cores of the chickens, and mixing to obtain turbid liquid;
(2) Putting the turbid liquid into an ultrasonic machine, carrying out ultrasonic treatment for 20 minutes under the ultrasonic power of 500W and the frequency of 40KHz, then sieving by a 300-mesh sieve, centrifuging for 1 minute at the rotating speed of 2000r/min, and collecting precipitates;
(3) Adding saturated saline solution into the precipitate according to the liquid-solid ratio of 2;
(4) Water and an oocyst floating solution are mixed according to the volume ratio of 10:1, mixing, centrifuging for 2 minutes at the rotating speed of 2500r/min, and collecting the precipitate to obtain the coccidian oocysts.
The resulting coccidial oocysts were counted.
Comparative example 2
A method for separating coccidian oocysts comprises the following steps:
(1) Mixing 100kg of feces containing cecum cores with 120L of water to obtain feces liquid, manually rubbing and kneading the feces liquid, adding water, sieving with a 300-mesh sieve, and collecting residues;
(2) Continuing to manually rub and knead the residue, adding water and sieving, repeating for 3-4 times, and collecting filtrate;
(3) Centrifuging the filtrate for 1-2 minutes at the rotating speed of 2000 revolutions, removing supernatant, and collecting precipitate;
(4) Adding saturated saline solution into the precipitate, fully stirring uniformly, centrifuging for 2-3 minutes at 3000 revolutions, and collecting oocyst floating liquid;
(5) Adding 8 times of water into the collected oocyst floating solution, centrifuging for 2-3 minutes at the rotating speed of 2500 revolutions, and collecting precipitates, wherein the precipitates are coccidian oocysts.
The resulting coccidial oocysts were counted.
And (4) supplementary notes: excrement treatment method and McLeod counter counting operation method
1.1 fecal treatment method the collected fecal samples were thoroughly stirred and mixed well.
1.2 McLeod counting operation method adopting random multipoint sampling method (sampling points are not less than 3) to weigh 2g of uniformly stirred feces, placing the feces in a clean beaker, adding 60ml of saturated saline, filtering the feces in another clean beaker through a 40-mesh screen after mixing uniformly, sucking filtrate after blowing uniformly by a rubber head dropper to fill two counting chambers of the McLeod counting plate, standing for 2-5 min, and calculating the total oocyst number (a 1) of the two counting chambers under 10 x 10 times of microscope; the sampling operation is repeated once, the total number of oocysts (a 2) is calculated, and the average value (a) of a1 and a2 is multiplied by 100 to obtain the number of oocysts per gram of feces (OPG value).
2. The operation method and the counting method for counting the oocysts by using a blood counting plate are as follows: fully and uniformly stirring the coccidian oocyst suspension to be detected, dropping the sample into a blood counting chamber by using a sample injector, counting the total number of sporulated oocysts in 4 angular squares by using a 10X 10-fold microscope, and multiplying the total number by 2.5X 10 3 This is the sporulated oocyst count in 1ml of oocyst suspension.
3. Oocyst recovery (%) = actual oocyst collection ÷ expected oocyst collection × 100%.
Application experiments
The methods of examples 1-7 and comparative examples 1-2 were applied to the isolation of coccidial oocysts and the time required for isolation and the total number of oocysts obtained were recorded, and the results are shown in Table 1.
TABLE 1
And (4) conclusion:
(1) Compared with the prior art (comparative example 2), the time for separating the same amount of excrement by the method used in the examples 1-7 is shortened by 54-78 minutes, and the separation efficiency is improved by 20.00-28.89%; the method used in the examples 1, 3 and 5 to 7 shortens 24 to 42 minutes compared with the separation method only using ultrasonic waves (comparative example 1), and the separation efficiency is improved by 10.26 to 17.95 percent; compared with the prior art or only ultrasonic waves are used, the method provided by the invention has the advantages that the separation time can be obviously shortened, the separation efficiency is improved, and the combined use effect of the excrement treating agent and the ultrasonic waves is more obvious.
(2) The oocysts obtained by separating the same amount of excrement by the method used in the examples 1 to 7 are increased by 46.4 to 48.8 hundred million compared with the prior art (comparative example 2), and the recovery rate of the oocysts is improved by 30.8 to 32.5 percent compared with the prior art; examples 1, 3 and 5-7 the number of oocysts harvested using the method is 21.2-23.4 billion greater than that harvested using ultrasound alone (comparative example 1), and the recovery rate of oocysts is improved by 12.0% -13.4%; compared with the prior art or the method only using ultrasonic waves to obtain the oocyst amount, the method has the advantages that the excrement treating agent is added and the ultrasonic waves are used, the combined use effect of the excrement treating agent and the ultrasonic waves is more obvious, and the oocyst recovery rate is higher.
While the fecal management agent of the present invention and the method and use thereof for the treatment of cecal core-containing feces of chickens have been described in detail above, the principles and embodiments of the present invention are described herein using specific examples, which are set forth only to aid in the understanding of the methods of the present invention and their core concepts, including the best mode, and to enable any person skilled in the art to practice the invention, including making and using any devices or systems and performing any combination thereof. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, it is possible to make various improvements and modifications to the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention. The scope of the invention is defined by the claims and may include other embodiments that occur to those skilled in the art. Such other embodiments are intended to be within the scope of the claims if they have structural elements that do not differ from the literal language of the claims, or if they include equivalent structural elements with insubstantial differences from the literal languages of the claims.
Claims (5)
1. A method for treating excrement containing chicken cecum cores by using an excrement treating agent is characterized by comprising the following steps of:
(1) Adding water into excrement containing the cecum core of the chicken for mixing to obtain excrement liquid, then adding an excrement treating agent for treating the excrement liquid, and standing to obtain turbid liquid;
(2) Carrying out ultrasonic treatment on the turbid liquid; the power of ultrasonic treatment is 300-600W, the frequency of ultrasonic treatment is 20-40KHz, and the time is 10-30min; sieving the turbid solution, centrifuging, and collecting precipitate to obtain the final product; the excrement treating agent comprises the following components in parts by weight: 5-10 parts of sodium hypochlorite, 3-6 parts of subtilisin, 5-10 parts of cellulase and 10-15 parts of water-soluble auxiliary agent, wherein the water-soluble auxiliary agent is at least one of polyethylene glycol 300, polyethylene glycol 1000, polyethylene glycol 2000, polyethylene glycol 4000 and polyethylene glycol 6000.
2. The method according to claim 1, wherein the step (2) further comprises adding a saturated saline solution to the precipitate, stirring, centrifuging, and collecting a supernatant; adding water into the supernatant, centrifuging, and collecting the centrifugal precipitate to obtain the coccidian oocysts.
3. The method according to claim 1, wherein in the step (1), the mass ratio of the fecal treatment agent to the feces containing chicken cecal core is 1 (100-120).
4. The method according to claim 1, wherein in the step (1), the standing time is 10-30min.
5. The method according to claim 2, wherein the liquid-solid ratio of the saturated brine to the precipitate is (1-3) 1L/kg; the volume ratio of the supernatant to the water is 1 (5-10).
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| NZ227597A (en) * | 1988-01-15 | 1991-11-26 | Merck & Co Inc | Eimeria tenella group b immunogens and their production |
| US7087425B2 (en) * | 2004-03-31 | 2006-08-08 | Embrex, Inc. | Method of purifying oocysts using enzymatic digestion of fecal debris |
| CN102174408B (en) * | 2011-01-10 | 2012-08-22 | 内蒙古神元生物工程股份有限公司 | Method for batch preparation of chicken Eimeria coccidium oocysts |
| CN107022489B (en) * | 2016-01-29 | 2020-10-27 | 天津赫莱恩特生物科技有限公司 | Solution for improving recovery rate of coccidian oocysts in chicken manure and preparation method thereof |
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