CN107913785A - A kind of efficiently animal tendon breaking method - Google Patents
A kind of efficiently animal tendon breaking method Download PDFInfo
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- CN107913785A CN107913785A CN201610893946.4A CN201610893946A CN107913785A CN 107913785 A CN107913785 A CN 107913785A CN 201610893946 A CN201610893946 A CN 201610893946A CN 107913785 A CN107913785 A CN 107913785A
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- heel string
- crushing
- string class
- collagen
- heel
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- 238000000034 method Methods 0.000 title claims abstract description 40
- 241001465754 Metazoa Species 0.000 title claims abstract description 20
- 210000002435 tendon Anatomy 0.000 title claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 49
- 102000008186 Collagen Human genes 0.000 claims abstract description 48
- 108010035532 Collagen Proteins 0.000 claims abstract description 48
- 229920001436 collagen Polymers 0.000 claims abstract description 40
- 238000007710 freezing Methods 0.000 claims abstract description 38
- 230000008014 freezing Effects 0.000 claims abstract description 37
- 239000000843 powder Substances 0.000 claims abstract description 31
- 239000011780 sodium chloride Substances 0.000 claims abstract description 19
- 239000000725 suspension Substances 0.000 claims abstract description 19
- 210000003195 fascia Anatomy 0.000 claims abstract description 14
- 238000005119 centrifugation Methods 0.000 claims abstract description 11
- 238000004519 manufacturing process Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 210000003205 muscle Anatomy 0.000 claims abstract description 7
- 241001494479 Pecora Species 0.000 claims description 11
- 239000004519 grease Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- 238000010612 desalination reaction Methods 0.000 claims description 2
- 238000003912 environmental pollution Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 239000007789 gas Substances 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- 238000011017 operating method Methods 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 238000005057 refrigeration Methods 0.000 claims description 2
- SDLBJIZEEMKQKY-UHFFFAOYSA-M silver chlorate Chemical compound [Ag+].[O-]Cl(=O)=O SDLBJIZEEMKQKY-UHFFFAOYSA-M 0.000 claims description 2
- 229910001961 silver nitrate Inorganic materials 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 238000005660 chlorination reaction Methods 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 238000002203 pretreatment Methods 0.000 claims 1
- 230000005855 radiation Effects 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 230000005611 electricity Effects 0.000 abstract 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 12
- 239000002253 acid Substances 0.000 description 9
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 102000057297 Pepsin A Human genes 0.000 description 2
- 108090000284 Pepsin A Proteins 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 229940111202 pepsin Drugs 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 239000003519 biomedical and dental material Substances 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000000744 eyelid Anatomy 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 208000033065 inborn errors of immunity Diseases 0.000 description 1
- 210000003041 ligament Anatomy 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 208000028529 primary immunodeficiency disease Diseases 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 210000000515 tooth Anatomy 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C21/00—Disintegrating plant with or without drying of the material
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Water Supply & Treatment (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of simple and feasible heel string class to crush, except oil and fat method, the heel string class powder crushed through the method is suitable for extracting collagen, and collagen keeps complete triple-helix structure, available for preparing collagen protein sponge and collagen Related product.This method key step：After the animal heel string mechanical removal fascia, muscle and the fat that will be enriched in collagen, rinsed with water or sodium chloride solution, freezing.The animal heel string after freezing hardening is taken out, the remaining fascia on heel string surface is scraped off with blade, is thinly sliced heel string with slicer.By heel string class rolls of sheets into strips, shave again after freezing is hardened.Heel string class thin slice is put into certain density sodium chloride solution, is crushed with pulverizer or grinder, up to heel string class suspension.Heel string class suspension is centrifuged, discards saline solution, rinsing residue, centrifugation, the heel string class powder after must crushing.Breaking method provided by the present invention is simple and practicable, economizing on energy and electricity, and production efficiency is high, is suitable for industrialized production.
Description
Technical field
The present invention relates to biotechnology, medical instrument, daily-use chemical industry technical field, more particularly to animal tendon breaking method
Method and its application.
Background technology
Collagen is widely present in the animal connective tissues such as tendon, ligament, cartilage, skin, tooth, plays eyelid retractor
Official, assigns tissue mechanical intensity, to the breaking up of cell, it is mobilisation tend to, the reparation of connective tissue plays an important role.Collagen egg
Natural Bai Yiqi hemostasis, hypotoxicity, low antigenicity, hypoimmunity, can guide cytothesis and with human compatibility preferably etc.
Advantage, and it is widely used in biomedical material and clinical doctor.
Since tendon tissue toughness is strong, do not make specially treated, section is difficult with crushing, and directly affects the extraction of collagen
Efficiency.Since heel string class derives from animal, with the presence of a small amount of grease type, emulsification is also easy to produce, is unfavorable at the purifying of collagen
Reason.Acetone, ethanol, n-butanol immersion grease removal can be used, but operating method is cumbersome, environment easy to pollute, organic solvent residual etc. lack
Fall into.
Observed through repeatedly research, be easy to thinly slice heel string using freezing method, using sodium chloride solution wet method powder
Heel string can be efficiently ground into the heel string class powder of required all kinds of particle sizes by broken method；In addition, using sodium chloride solution
Wet pulverizing method effectively can remove the grease in heel string.
The content of the invention
It is an object of the invention to provide a kind of efficient pulverizing skill for the shortcoming in existing heel string crushing technology
Art, the crushing technology energy-saving and environmental protection.The purpose of the present invention, which is lain also in, to be removed and provides easy-to-use for the grease in heel string
Removing method, is suitable for industrialized production.
To reach above-mentioned purpose, the present invention is achieved through the following technical solutions.
Animal heel string and the method for removing grease in heel string are effectively crushed, is mainly included the following steps that：
Step 1, after the animal heel string mechanical removal fascia, muscle and the fat that will be enriched in collagen, with water or sodium chloride
Solution rinsed clean, freezing.
Step 2, takes out the animal heel string after freezing hardening, the remaining fascia on heel string surface is scraped off with blade, uses slicer
Heel string is thinly sliced.
Step 3, by heel string class rolls of sheets into strips, shave again after freezing is hardened；
Poly- four are walked, heel string class thin slice is put into certain density sodium chloride solution, is crushed with pulverizer or grinder, i.e.,
Obtain heel string class suspension.
Step 5, heel string class suspension is centrifuged, and discards saline solution, rinsing residue, centrifugation, the heel string class powder after must crushing
End.
In such scheme, after the step 5, can by the heel string powder air drying after crushing, be dried under reduced pressure, freeze
Pack, be put in storage after drying.Also can be by the collagen powder of above-mentioned preparation through Co60Radiation sterilization, is then packed, storage.
In such scheme, in the step 1, animal heel string includes ox heel string, pig heel string, sheep heel string and horse heel string etc. and appoints
Any one in what animal heel string or two or more compositions.
In such scheme, in the step 1 freezing be that zero is Celsius or zero degrees celsius below freeze, it is common for refrigerator or
The freezing of refrigerator-freezer zero degrees celsius, subzero 10 degrees Celsius of freezings, subzero 20 degrees Celsius of freezings, subzero 50 degrees Celsius of freezings, subzero 80 are taken the photograph
Family name's degree freezes.
In such scheme, in the step 1 freezing be that zero is Celsius or zero degrees celsius below freeze, also include using
Special liquid or gas refrigeration method, such as use liquid nitrogen quick-freezing method.
In such scheme, in the step 1 freezing be that zero is Celsius or zero degrees celsius below freeze, cooling time can be grown can
It is short, reach the hardness of required section.
In such scheme, blade can be seen for knife blade or in the market in the step 2 any sharp instrument.
In such scheme, in the step 4, the concentration of sodium chloride solution can reach supersaturation concentration, common to be
0.9% concentration.
In such scheme, in the step 5, by heel string class suspension centrifuge, centrifugation refer to using various centrifuges,
Refrigerated centrifuge, its centrifugal speed is rapidly more than 100rpm to 20000rpm.
In such scheme, in the step 5, saline solution, rinsing residue, centrifugation, the heel string class powder after must crushing are discarded.
The degree of washing can use silver nitrate solution titration aqueous, such as produce silver chlorate flocculent deposit, then it represents that desalination it is unclean, it is necessary to
Continue to clean.
In such scheme, in the step 5, saline solution is discarded, the heel string class powder after must crushing.If without centrifuge,
Also required heel string class powder can be obtained directly using the program of filtering, then be dried through suitable method.
Beneficial effects of the present invention：Production cost is low, and labor intensity is small, free from environmental pollution, is suitable for industrialized production；Institute
The collagen powder or suspension of preparation are conducive to the extraction of collagen, and are prepared into the various doctors using collagen as raw material
Treat apparatus, preparation, daily chemical products.
Brief description of the drawings
Fig. 1 is heel string powder prepared in preparation method provided by the present invention
Fig. 2 is extracted rear prepared collagen protein sponge (the laboratory lab scale of the heel string powder prepared using the present invention
Sample)
Fig. 3 is that the extracted rear prepared collagen protein sponge of the heel string powder prepared using the present invention (put by workshop pilot scale
Big production gained sample)
Fig. 4 is the extracted rear prepared collagen liquid of the heel string powder prepared using the present invention
Fig. 5 is the extracted rear prepared collagen dressing of the heel string powder prepared using the present invention
Fig. 6 is the gel egg of the extracted rear obtained collagen solution of the ox heel string powder prepared using the present invention
White electrophoretogram
Embodiment
In order to which technical problem, technical solution and beneficial effect solved by the invention is more clearly understood, below in conjunction with
Embodiment, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only explaining
The present invention, is not intended to limit the present invention.
Embodiment 1.
Ox heel string and the method for removing grease in ox heel string are effectively crushed, is mainly included the following steps that：
Step 1, after removing ox heel string machinery fascia, muscle and fat, with water or sodium chloride solution rinsed clean, freezing.
Step 2, takes out the ox heel string after freezing hardening, and the remaining fascia on heel string surface is scraped off with blade, will with slicer
Heel string is thinly sliced.
Step 3, by ox heel string rolls of sheets into strips, shave again after freezing is hardened；
Poly- four are walked, ox heel string thin slice is put into certain density sodium chloride solution, is crushed with pulverizer or grinder, i.e.,
Obtain ox heel string suspension.
Step 5, ox heel string suspension is centrifuged, and discards saline solution, rinsing residue, centrifugation, the ox heel string albumen after must crushing
Powder.
1 N of heel string breaking method of the present embodiment, simple and effective, production cost is low, can be suitable for mass producing and wide
The characteristics of general popularization.
Embodiment 2.
Sheep heel string and the method for removing grease in sheep heel string are effectively crushed, is mainly included the following steps that：
Step 1, after removing sheep heel string machinery fascia, muscle and fat, with water or sodium chloride solution rinsed clean, freezing.
Step 2, takes out the sheep heel string after freezing hardening, and the remaining fascia on heel string surface is scraped off with blade, will with slicer
Heel string is thinly sliced.
Step 3, by sheep heel string rolls of sheets into strips, shave again after freezing is hardened；
Poly- four are walked, sheep heel string thin slice is put into certain density sodium chloride solution, is crushed with pulverizer or grinder, i.e.,
Obtain sheep heel string suspension.
Step 5, sheep heel string suspension is centrifuged, and discards saline solution, rinsing residue, centrifugation, the sheep heel string albumen after must crushing
Powder.
Embodiment 3.
Horse heel string and the method for removing grease in horse heel string are effectively crushed, is mainly included the following steps that：
Step 1, after removing horse heel string machinery fascia, muscle and fat, with water or sodium chloride solution rinsed clean, freezing.
Step 2, takes out the horse heel string after freezing hardening, and the remaining fascia on heel string surface is scraped off with blade, will with slicer
Heel string is thinly sliced.
Step 3, by horse heel string rolls of sheets into strips, shave again after freezing is hardened；
Poly- four are walked, horse heel string thin slice is put into certain density sodium chloride solution, is crushed with pulverizer or grinder, i.e.,
Obtain horse heel string suspension.
Step 5, horse heel string suspension is centrifuged, and discards saline solution, rinsing residue, centrifugation, the horse heel string albumen after must crushing
Powder.
Embodiment 4.
Pig heel string and the method for removing grease in pig heel string are effectively crushed, is mainly included the following steps that：
Step 1, after removing pig heel string machinery fascia, muscle and fat, with water or sodium chloride solution rinsed clean, freezing.
Step 2, takes out the pig heel string after freezing hardening, and the remaining fascia on heel string surface is scraped off with blade, will with slicer
Heel string is thinly sliced.
Step 3, by pig heel string rolls of sheets into strips, shave again after freezing is hardened；
Poly- four are walked, pig heel string thin slice is put into certain density sodium chloride solution, is crushed with pulverizer or grinder, i.e.,
Obtain pig heel string suspension.
Step 5, pig heel string suspension is centrifuged, and discards saline solution, rinsing residue, centrifugation, the pig heel string albumen after must crushing
Powder.
Embodiment 5.
The preparation of collagen liquid and collagen dressing
Step 1, the ox heel string protein powder as obtained by inventive method are put in dilute acid soln, and into dilute acid soln
Add activity be suitable protease, then at 4~10 DEG C stir extract 48~72 it is small when after, obtain extracting solution；This implementation
Example in, diluted acid can acetic acid, citric acid, hydrochloric acid, citric acid, pH value≤3 of solution.In the present embodiment, enzyme can be pepsin, nothing
Flowers and fruits enzyme；Enzyme and heel string ratio 1000: 1-10: 1.
Step 3, is centrifuged and is collected supernatant to the extracting solution that step 2 obtains；
Step 4, appropriate sodium chloride solution is added into the supernatant obtained by step 3, collagen egg is separated out in supernatant
In vain, the collagen that quick washing separates out, that is, obtain the collagen of high-purity；
Step 5, collagen diluted acid or the water dissolving for the high-purity that step 4 is obtained, according to prepared product
It is required that it is different, appropriate pH or the suitable aromatic of addition are adjusted, suitable glycerine can be also added, to ensure that product is suitably thick
Degree, that is, be made collagen liquid or collagen dressing.
Step 6, the collagen liquid or collagen dressing obtained toward step 5 is different according to clinical practice, may be selected
Carry out Co to property60Radiation sterilization.
Step 7, gel protein electrophoresis is carried out to the collagen solution obtained in step 4.Occur three in swimming lane
Bar bands of a spectrum, have two at 100kDa, and one at 200kDa, corresponds respectively to 1 chains of α, the α in the micro- collagen structure of collagen
2 chains, β chains.It is consistent with the I-type collagen architectural feature in document.
Embodiment 6.
The preparation of collagen protein sponge
Step 1, the ox heel string protein powder as obtained by inventive method are put in dilute acid soln, and into dilute acid soln
Add activity be suitable protease, then at 4~10 DEG C stir extract 48~72 it is small when after, obtain extracting solution；This implementation
Example in, diluted acid can acetic acid, citric acid, hydrochloric acid, citric acid, pH value≤3 of solution.In the present embodiment, enzyme can be pepsin, nothing
Flowers and fruits enzyme；Enzyme and heel string ratio 1000: 1-10: 1.
Step 3, is centrifuged and is collected supernatant to the extracting solution that step 2 obtains；
Step 4, appropriate sodium chloride solution is added into the supernatant obtained by step 3, collagen egg is separated out in supernatant
In vain, the collagen that quick washing separates out, that is, obtain the collagen of high-purity；
Step 5, the collagen diluted acid back dissolving for the high-purity that step 4 is obtained, mould is poured into after adjusting concentration
In, it is dried under reduced pressure, up to collagen protein sponge.
Step 6, for improve sponge mechanical property, the collagen diluted acid back dissolving for the high-purity that step 4 is obtained,
Add suitable glutaraldehyde solution, be freeze-dried again after full cross-linked, that is, the collagen protein sponge after being crosslinked.
Step 7, Step 5: six obtained collagen protein sponges, Co is carried out after packaged60Radiation sterilization, it is spare.It is made
Collagen protein sponge, be loose porous sponge structure, and its color and luster is pure white, good appearance.
It should be noted that above example is merely to illustrate technical scheme rather than to the scope of the present invention
Limitation, although being explained in detail with reference to preferred embodiment to the present invention, it will be understood by those of ordinary skill in the art that, can
With to technical scheme technical scheme is modified or replaced equivalently, without departing from the spirit and scope of technical solution of the present invention.
Claims (15)
1. the crushing of animal heel string class, except oil and fat method, it is characterised in that：It comprises the following steps：
Step 1, after the animal heel string mechanical removal fascia, muscle and the fat that will be enriched in collagen, with water or sodium chloride solution
Rinsing, freezing.
Step 2, takes out the animal heel string after freezing hardening, the remaining fascia on heel string surface is scraped off with blade, will be with slicer
Tendon is thinly sliced.
Step 3, by heel string class rolls of sheets into strips, shave again after freezing is hardened；
Walk poly- four, heel string class thin slice be put into certain density sodium chloride solution, crushed with pulverizer or grinder, up to
Tendon class suspension.
Step 5, heel string class suspension is centrifuged, and discards saline solution, rinsing residue, centrifugation, the heel string class powder after must crushing.
2. heel string class breaking method according to claim 1, it is characterised in that：, can be by after crushing after the step 5
Heel string powder air drying, be dried under reduced pressure, be freeze-dried after pack, be put in storage.Also can be by the collagen powder of above-mentioned preparation through Co60
Radiation sterilization, is then packed, storage.
3. the crushing of heel string class according to claim 1, except oil and fat method, it is characterised in that：In the step 1, animal
Heel string includes any one or two or more groups in any animal heel strings such as ox heel string, pig heel string, sheep heel string and horse heel string
Compound.
4. the crushing of heel string class according to claim 1, except oil and fat method, it is characterised in that：Freezed in the step 1
Be that zero is Celsius or zero degrees celsius below freeze, it is common for refrigerator or the freezing of refrigerator-freezer zero degrees celsius, subzero 10 degrees Celsius of freezings, zero
Lower 20 degrees Celsius of freezings, subzero 50 degrees Celsius of freezings, subzero 80 degrees Celsius of freezings.
5. the crushing of heel string class according to claim 1, except oil and fat method, it is characterised in that：Freezed in the step 1
Be that zero is Celsius or zero degrees celsius below freeze, also include use some special liquids or gas refrigeration method, such as use liquid nitrogen fast
Fast freezing method.
6. the crushing of heel string class according to claim 1, except oil and fat method, it is characterised in that：It is described Step 1: step 2
Middle freezing be that zero is Celsius or zero degrees celsius below freeze, cooling time is changeable, reach needed for section hardness.
7. the crushing of heel string class according to claim 1, except oil and fat method, it is characterised in that：Blade in the step 2
Any sharp instrument that can be seen for knife blade or in the market.
8. the crushing of heel string class according to claim 1, except oil and fat method, it is characterised in that：In the step 4, chlorination
The concentration of sodium solution can reach supersaturation concentration, it is common be 0.9% concentration.
9. the crushing of heel string class according to claim 1, except oil and fat method, it is characterised in that：, will be with the step 5
Tendon class suspension centrifuges, and centrifugation refers to arriving using various centrifuges, refrigerated centrifuge, its centrifugal speed 100rpm rapidly
More than 20000rpm.
10. the crushing of heel string class according to claim 1, except oil and fat method, it is characterised in that：In the step 5, abandon
Remove saline solution, rinsing residue, centrifugation, the heel string class powder after must crushing.The degree of washing can titrate aqueous with silver nitrate solution,
Such as produce silver chlorate flocculent deposit, then it represents that desalination is not clean, it is necessary to continue to clean.
11. the crushing of heel string class according to claim 1, except oil and fat method, it is characterised in that：In the step 5, abandon
Saline solution is removed, the heel string class powder after must crushing.If, also can be directly using the program of filtering without centrifuge, then pass through suitably
Method is dried, and obtains required heel string class powder.
12. animal heel string described in claim 1 to 12 any one can be the heel string of fresh materials, or it is dried with
Tendon.
13. the crushing of heel string class according to claim 1, except oil and fat method, it is characterised in that：Above-mentioned steps one are to step
In five, some steps, which can be saved, can also be repeated several times progress.As step 2 can be saved, it is only necessary to once cut into slices.
Can also multiple freezing microtome section, to reach more preferable crushing effect.
14. the crushing of heel string class according to claim 1, except oil and fat method, it is characterised in that：Above-mentioned steps one are to step
Five be basic operating procedure, heel string class can also be carried out some pre-treatments, and alcohol is used before and after can animal heel string be cut into slices
Immersion, acetone or n-butanol immersion, tentatively grease effect is removed to reach.
15. powder or suspension that the animal heel string class breaking method described in claim 1 to 14 any one is obtained, it is special
Sign is：Production cost is low, and labor intensity is small, free from environmental pollution, is suitable for industrialized production；Prepared collagen powder or mixed
Suspension is conducive to the extraction of collagen, and the various medical instruments, preparation, daily use chemicals that are prepared into using collagen as raw material produce
Product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN111214709A (en) * | 2020-03-27 | 2020-06-02 | 中国医科大学 | Novel double-layer nerve conduit and preparation method thereof |
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