CN114375335B - 经优化的蛋白质连接体以及使用方法 - Google Patents

经优化的蛋白质连接体以及使用方法 Download PDF

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CN114375335B
CN114375335B CN202080064288.2A CN202080064288A CN114375335B CN 114375335 B CN114375335 B CN 114375335B CN 202080064288 A CN202080064288 A CN 202080064288A CN 114375335 B CN114375335 B CN 114375335B
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CN114375335A (zh
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S·L·顾非
J·M·沃茨
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Pairing Plant Service Co ltd
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21001—Deoxyribonuclease I (3.1.21.1)
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    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04001—Cytosine deaminase (3.5.4.1)
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    • C07K2319/00—Fusion polypeptide
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3513—Protein; Peptide
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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  • Plant Pathology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202080064288.2A 2019-07-19 2020-07-17 经优化的蛋白质连接体以及使用方法 Active CN114375335B (zh)

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CN202510765287.5A CN120591235A (zh) 2019-07-19 2020-07-17 经优化的蛋白质连接体以及使用方法

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US201962876275P 2019-07-19 2019-07-19
US62/876,275 2019-07-19
PCT/US2020/042553 WO2021016086A1 (en) 2019-07-19 2020-07-17 Optimized protein linkers and methods of use

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US (2) US11072785B2 (OSRAM)
EP (1) EP3987021A4 (OSRAM)
JP (2) JP2022541273A (OSRAM)
CN (2) CN114375335B (OSRAM)
AU (1) AU2020316331B2 (OSRAM)
CA (1) CA3147253A1 (OSRAM)
WO (1) WO2021016086A1 (OSRAM)

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AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
US11072785B2 (en) * 2019-07-19 2021-07-27 Pairwise Plants Services, Inc. Optimized protein linkers and methods of use
US11718838B2 (en) 2020-07-21 2023-08-08 Pairwise Plants Services. Inc. Optimized protein linkers and methods of use
US12123006B2 (en) * 2021-05-18 2024-10-22 Shanghaitech University Base editing tool and use thereof
US20230287456A1 (en) * 2021-09-10 2023-09-14 Arbor Biotechnologies, Inc. Compositions comprising a cas12i polypeptide and uses thereof
US20230383271A1 (en) * 2022-02-28 2023-11-30 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
CN116217741B (zh) * 2023-02-27 2025-06-27 河北科技大学 一种高效率且低脱靶率的精确基因编辑方法
US12428636B1 (en) 2024-07-08 2025-09-30 Pairwise Plants Services, Inc. Methods and compositions for modification of protospacer adjacent motif specificity of CAS12A

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Also Published As

Publication number Publication date
CN120591235A (zh) 2025-09-05
EP3987021A4 (en) 2024-05-15
WO2021016086A1 (en) 2021-01-28
US11072785B2 (en) 2021-07-27
US20210017506A1 (en) 2021-01-21
US20210355466A1 (en) 2021-11-18
CA3147253A1 (en) 2021-01-28
US11708568B2 (en) 2023-07-25
JP2025020231A (ja) 2025-02-12
CN114375335A (zh) 2022-04-19
EP3987021A1 (en) 2022-04-27
JP2022541273A (ja) 2022-09-22
AU2020316331B2 (en) 2026-02-12
AU2020316331A1 (en) 2022-02-03

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