CN114369538A - Trichoderma asperellum preying on banana fusarium wilt and application thereof - Google Patents
Trichoderma asperellum preying on banana fusarium wilt and application thereof Download PDFInfo
- Publication number
- CN114369538A CN114369538A CN202111570689.8A CN202111570689A CN114369538A CN 114369538 A CN114369538 A CN 114369538A CN 202111570689 A CN202111570689 A CN 202111570689A CN 114369538 A CN114369538 A CN 114369538A
- Authority
- CN
- China
- Prior art keywords
- trichoderma asperellum
- foc4
- trichoderma
- banana
- asperellum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides Trichoderma asperellum for predating banana fusarium oxysporum and application thereof, wherein the Trichoderma asperellum is named as Trichoderma asperellum (Trichoderma asperellum) MM7 and is preserved in China Center for Type Culture Collection (CCTCC) in 3-30 months in 2021, and the preservation number is CCTCC NO: m2021302. The trichoderma asperellum MM7 can enable pathogenic bacteria filaments of the No. 4 physiological race (Foc4) of banana vascular wilt pathogens to obviously have the phenomena of scarcity, breakage, dissolution and the like, destroy Foc4 pathogen cells, has strong predation effect on Foc4, provides a new thought and a biological control resource for the prevention and control of banana vascular wilt diseases, and has wide development space and good development and application prospects.
Description
Technical Field
The invention belongs to the field of microorganisms, and particularly relates to trichoderma asperellum for predating banana vascular wilt and application thereof.
Background
Bananas are one of the most important fruits in tropical and subtropical zones (Dita et al, 2018). Its prime producing area is asia and central and south america, is the second largest fruit crop in the world with yields second only to citrus, providing a staple food for approximately 4 billion populations worldwide (dushuncili, 2017). When the banana industry develops, the occurrence and spread of banana vascular wilt severely restrict the development of the banana industry and bring great economic loss to the banana industry.
The banana wilt is a soil-borne disease causing destructive damage to the banana industry, and no effective chemical agent is available for prevention and treatment at present. Biological control is a leading-edge technology developed in recent years, has the advantages of environmental friendliness, no side effect on bananas, simple application technology and the like (wang army, 2014), and is considered to be a promising control strategy for banana wilt compared with different control strategies. Therefore, the development of pollution-free and pollution-free biological control of banana vascular wilt becomes one of the important breakthrough.
Trichoderma is an important microbial resource and has important application value in preventing and treating agricultural diseases and insect pests. The research separates trichoderma from rhizosphere soil of banana garden, and the banana wilt is used as the target bacterium to carry out predation screening, so as to provide new thinking and biocontrol resources for prevention and control of banana wilt.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides trichoderma capable of predating No. 4 physiological races of banana vascular wilt and providing a new thought and a new biocontrol resource for prevention and control of banana vascular wilt.
The first aspect of the invention provides Trichoderma asperellum, named Trichoderma asperellum (Trichoderma asperellum) MM7, which is preserved in China Center for Type Culture Collection (CCTCC) at 3 months and 30 days 2021, with the preservation number of CCTCC NO: m2021302.
In a second aspect of the present invention, there is provided use of Trichoderma asperellum according to the first aspect of the present invention in the predation of physiological race # 4 of Fusarium oxysporum f.sp.
The third aspect of the invention provides the application of the trichoderma asperellum in the preparation of a biocontrol agent for preventing and treating diseases caused by No. 4 physiological races of banana vascular wilt.
A fourth aspect of the present invention provides the use of Trichoderma asperellum according to the first aspect of the present invention in the preparation of a preparation for predating No. 4 physiological race hyphae of Fusarium oxysporum F.sp.
In a fifth aspect of the present invention, there is provided use of Trichoderma asperellum according to the first aspect of the present invention in the preparation of a preparation for predating cells of physiological race 4 of Fusarium oxysporum f.sp.
In a sixth aspect of the present invention, there is provided a biocontrol bacterium preparation capable of preying on physiological race # 4 of banana vascular wilt bacteria, comprising Trichoderma asperellum according to the first aspect of the present invention.
The trichoderma asperellum MM7 can enable pathogenic bacteria filaments of the No. 4 physiological race (Foc4) of banana vascular wilt pathogens to obviously have the phenomena of scarcity, breakage, dissolution and the like, destroy Foc4 pathogen cells, has strong predation effect on Foc4, provides a new thought and a biological control resource for the prevention and control of banana vascular wilt diseases, and has wide development space and good development and application prospects.
Drawings
FIG. 1 shows the observation of Trichoderma asperellum MM7 by scanning electron microscope after 3 days of culture, 2 μm scale.
FIG. 2 is a phylogenetic tree constructed based on ITS adjacency.
FIG. 3 is an agar dot blot assay to determine the predation effect of Trichoderma asperellum MM7 on Foc 4.
FIG. 4 is a cross-shaped vergence method for determining the predation effect of Trichoderma asperellum MM7 on Foc 4.
FIG. 5 shows the predation effect of Trichoderma asperellum MM7 on Foc4 as determined by the slide method.
FIG. 6 is a scanning electron microscope observation of the predation effect of banana vascular wilt hypha after trichoderma asperellum MM7 treatment; wherein a is untreated Foc4 hyphae; b is Foc4 hyphae treated with trichoderma asperellum MM 7.
FIG. 7 is transmission electron microscope observation of Trichoderma asperellum MM7 on the ultrastructure of banana vascular wilt hyphae; wherein a is a hyphal cell of untreated Foc4, and is in a normal cell structure state; b and c are ultrastructural changes of Foc4 cells after treatment with trichoderma asperellum MM 7.
Detailed Description
The invention will be better understood by reference to the following examples.
The invention provides Trichoderma asperellum, named Trichoderma asperellum MM7, which is preserved in China Center for Type Culture Collection (CCTCC) at 3 months and 30 days in 2021, wherein the preservation number is CCTCC NO: m2021302, address Wuhan university in Wuhan, China. The trichoderma asperellum MM7 is collected from banana rhizosphere soil collected from Hei-Gao county in Hainan province in China.
1 test Material
1.1 test samples
Collecting rhizosphere soil of bananas in Lingao county of Hainan province, placing in a sterile sealing bag, mixing, sealing, and storing in an ice box. Collecting, removing impurities such as root system and stone, and storing in refrigerator at 4 deg.C for use.
1.2 test Medium
The main culture media used in the experiments in this chapter include isolation medium and physiological and biochemical characteristic observation medium, which are shown in tables 1 and 2.
TABLE 1 composition of the isolation Medium
1.3 Primary reagents
The main reagents used in the test of this chapter are shown in Table 2.
TABLE 2 major Biochemical reagents and sources
1.4 Experimental instruments and apparatus
The main instruments and equipment required for the test in this chapter are shown in Table 3.
TABLE 3 instruments and apparatus
1.5 test pathogens
The physiological Race F.oxysporum f.sp.cubense Race 4(ATCC 76255) (Foc4) of banana fusarium oxysporum f.sp.cubense is stored in the research laboratory and is used for antifungal activity screening and antibacterial activity determination.
1.6 analytical software
The data analysis software used in this study is shown in table 4.
TABLE 4 analysis software and web site
2 test method
2.1 isolation of Trichoderma from the soil of the Banana park
Weighing 5g of fresh soil sample, adding 45mL of sterile water, placing on a shaking table at 180r/min, oscillating for 30min, and fully and uniformly mixing to obtain a suspension. Diluting by 10-fold continuous dilution method to obtain 10-1、10-2、10-3Taking 100 mu L of each gradient of the suspension, coating the suspension on a specific isolation medium of a Bengal culture medium, carrying out inverted culture at 28 ℃ for 1-2 weeks, setting 3 times of each gradient, and picking a single colony of trichoderma to streak-purify on a PDA culture medium after the colony grows out.
2.2 taxonomic identification of active strains
2.2.1 morphological characteristics observed by scanning Electron microscope
Taking 2mL of trichoderma liquid cultured to logarithmic phase, centrifuging at 6000rpm for 1min, washing with 0.1M phosphate buffer solution for three times, adding 2.5% glutaraldehyde, mixing uniformly, and standing overnight at 4 ℃; centrifuging to remove glutaraldehyde, washing twice with PBS for 15min, performing gradient dehydration on the thallus with 30%, 50%, 70%, 80%, 90%, 100% ethanol for 15min, wherein 100% dehydration treatment is performed twice for 20min, treating with mixed solution (v/v) of anhydrous ethanol and isoamyl acetate at a ratio of 1:1 for 30min, replacing for 2h with isoamyl acetate, coating on a glass slide, and observing with a scanning electron microscope after gold spraying.
2.2.2 molecular biological identification
Extraction and purification of trichoderma DNA and ITS sequence amplification
DNA was extracted using the CTAB (hexadecyltrimethylammonium Bromide, Hexadecyl trimethynium Bromide) method: putting a small amount of quartz sand into a 1.5mL Ep tube, adding 400 mu L of 2% CTAB, selecting a proper amount of thalli, and carrying out warm bath at 65 ℃ for 40-60 min (uniformly mixing the middle part upside down) and oscillation. ② adding 400 mu LDNA to extract the phenol mixed solution, covering the pipe orifice tightly, reversing and mixing evenly. ③ centrifugation at 12000rpm for 15min, and the supernatant was taken in a new 1.5mLEp tube. Fourthly, adding isopropanol with the same volume as the isopropanol, and standing for 30min at the temperature of minus 20 ℃. Fifthly, centrifuging the frozen sample in a centrifugal tube at 12000rpm for 10min, discarding the supernatant, and collecting the precipitated DNA. Sixthly, adding 50Rinsing with 0 μ L70% ethanol, centrifuging at 12000rpm for 2min, discarding supernatant, and air drying. Seventhly, adding 30 mu L of ddH containing RNase2And O, carrying out warm bath at 37 ℃ for 30 min. Performing electrophoretic detection, and storing the rest samples at-20 ℃.
The DNA obtained by extraction was subjected to PCR amplification by primers ITS1 and ITS4(ITS1:5'-TCCGTAGGTGAACCTGCGG-3', ITS4: 5'-TCCTCCGCTTATTGATATGC-3'). The reaction system for PCR is shown in Table 5. The reaction conditions for PCR amplification are shown in Table 6
TABLE 5 ITS sequences PCR reaction System
TABLE 6 ITS sequences PCR amplification reaction conditions
Secondly, electrophoresis detection of PCR products:
after the PCR reaction is finished, 5 mu L of PCR amplification product is taken to carry out electrophoresis detection on the PCR product of the strain on 1% agarose gel, and whether the connection is successful is determined according to the length of the target fragment.
Sequencing and sequence alignment analysis:
and (3) carrying out sequence determination on the PCR product of the strain. The determined ITS gene sequence was compared for homology to known ITS sequences stored in the public databases GenBank and NCBI databases.
2.3 predation Effect of Trichoderma on Foc4
2.3.1 preliminary screening for predation
And (3) determining the predation effect of the trichoderma by using the banana fusarium wilt bacteria as target bacteria and adopting a point inoculation culture method. Using a puncher with the diameter of 5mm to take a fungus cake of Foc4 pathogenic bacteria with consistent growth vigor, inoculating purified trichoderma on one end of each PDA plate at a position 5cm away from pathogenic bacteria colonies, placing the plate in an incubator at 28 ℃ for inverted culture for 14 days, using the pathogenic bacteria inoculated with Foc4 alone as a control, observing predation results of the trichoderma, and calculating the bacteriostasis rate.
The bacteriostatic ratio (%) - (control colony diameter-diameter of trichoderma inoculated colony)/control colony diameter 100%
2.3.2 Re-sifting for predation
Cutting PDA culture medium into 2.5cm wide cross shape, inoculating Foc4 pathogenic bacteria cake 3cm away from the center, inoculating Trichoderma at the center, repeating each treatment for three times, and culturing at 28 deg.C upside down for 14 d. The predation effect of trichoderma was observed.
2.4 predation of pathogenic hyphae attached to the slide by Trichoderma asperellum MM7
Foc4 germ-free fungus cakes are inoculated on a PDA plate culture medium, a sterile cover glass is obliquely inserted at the periphery of the fungus cakes, and the culture is carried out at the constant temperature of 28 ℃ until Foc4 hyphae grow on the cover glass. Carefully taking out the cover glass with tweezers, placing the cover glass with the hypha attachment surface facing upwards, and dropping 50 μ L of the solution at a concentration of 1 × 107cfu/mL Trichoderma asperellum MM7, the treated slides were placed in a sterile petri dish and incubated at 28 ℃ for 48h with hyphae added with sterile water as a control, and each treatment was repeated 3 times. The morphological changes of the hyphae of Foc4 pathogenic bacteria were observed using an optical microscope.
2.5 Observation of predation Effect of Trichoderma asperellum MM7 on Foc4 by scanning Electron microscopy
Using a puncher with the diameter of 5mm to take a fungus cake of Foc4 pathogenic bacteria with consistent growth vigor, inoculating to one end of each PDA plate, inoculating purified trichoderma at a position 5cm away from the pathogenic bacteria colony, placing in an incubator at 28 ℃ and culturing upside down for 5-7 days, and taking the pathogenic bacteria inoculated with Foc4 alone as a control. After the trichoderma asperellum MM7 and Foc4 are contacted, cutting a cube of the contact point, washing the cube with 0.1M phosphate buffer solution for three times, adding 2.5% glutaraldehyde, uniformly mixing, and standing at 4 ℃ overnight; removing glutaraldehyde by centrifugation, washing with PBS twice for 15min, performing gradient dehydration on the thallus with 30%, 50%, 70%, 80%, 90%, and 100% ethanol for 15min, wherein 100% dehydration treatment is performed twice for 20min, treating with a mixture (v/v) of anhydrous ethanol and isoamyl acetate at a ratio of 1:1 for 30min, replacing for 2h with isoamyl acetate, spreading on a glass slide, spraying gold, and observing the change of Foc4 germ hyphae by using a scanning electron microscope (SEM, model S-4800, Hitachi Limited, Japan).
2.6 Effect of Trichoderma asperellum MM7 on ultrastructure of Foc4 cells
A punch with the diameter of 5MM is used for taking a fungus cake of Foc4 pathogenic bacteria with consistent growth vigor, the fungus cake is inoculated to one end of each PDA plate, purified trichoderma asperellum MM7 is inoculated to a position 5cm away from a pathogenic bacteria colony, the PDA plates are placed in an incubator at the temperature of 28 ℃ for inverted culture for 5-7 days, and the control is that only Foc4 pathogenic bacteria are inoculated. After the trichoderma asperellum MM7 and Foc4 are contacted, cube pieces at the contact points are cut. The samples were dehydrated and then replaced 2 times for 15min each with propylene oxide. Embedding of the samples used propylene oxide: the epoxy resin (1:1) solution was soaked for 1h, trimmed to appropriate size and cut into 70nm sections using a microtome (handan, 2018). And (3) carrying out double dyeing on the cut sample by using a uranyl acetate solution and a lead citrate solution, and airing. The ultrastructure of Foc4 mycelial cells was observed by a transmission electron microscope (TEM, JEM-1400Flash, Hitachi Limited, Japan).
2.7 evaluation of prevention of banana vascular wilt by Trichoderma MM7
The test is carried out in a pot culture mode in a greenhouse of a tropical biotechnology research institute in 5 months to 7 months from 2021. The experiment was performed with 3 treatments, respectively: h2O: only applying clear water; foc 4: applying pathogenic bacteria Foc 4; t: after treatment with pathogenic bacteria, trichoderma MM7 was applied. 30 banana seedlings with 3-4 leaves with consistent growth conditions and health conditions are selected for each treatment.
After the potted plant experiment is carried out for two months, the total number of the leaves and the number of etiolated leaves of each treated banana seedling are counted, and the disease index and the prevention and control effect of bacterial manure of the banana seedlings are calculated according to the total number of the leaves and the number of etiolated leaves. Grading standard of disease index of banana wilt: grade 0, healthy plants; grade 1, with 25% yellowing leaves; grade 3, 25 to 50 percent of etiolation diseased leaves; grade 5, 50-90% of etiolation diseased leaves; and 7, all plants are etiolated and dead. The calculation formula of the disease index and the prevention and control effect of the banana vascular wilt is as follows:
disease index ═ Σ (number of diseased plants at each stage × number of diseased stages))/(number of total investigated plants × number of highest diseased stages) × 100%
The prevention and control effect is (contrast disease index-treatment disease index)/contrast disease index multiplied by 100 percent
3 results and analysis
3.1 taxonomic identification of strains
3.1.1 morphological feature analysis
Trichoderma MM7 was inoculated into PDA liquid medium and cultured at 28 ℃ for 3 days. Spore morphology observation was performed by Scanning Electron Microscopy (SEM) and is shown in FIG. 1. Trichoderma MM7 produced gray spore colonies with spikes on the surface.
3.1.2 molecular biological identification
(1) Extraction and PCR amplification of genomic DNA
The genomic DNA of trichoderma MM7 is extracted, and agarose gel electrophoresis results show that the target band of the genomic DNA is clearer, which indicates that the genomic DNA has higher purity and can be used as a template of PCR reaction. ITS PCR amplification is carried out by taking genome DNA as a template and ITS1 and ITS4 as primers to obtain a band of about 600 bp.
(2) ITS gene sequence analysis and phylogenetic relationship
The test strain adopts a clone sequencing method to obtain a complete ITS sequence, the sequence is submitted to an NCBI database, homology comparison is carried out on the sequence and related strains in NCBI (https:// www.ncbi.nlm.nih.gov /), 15 standard strains with higher homology are selected, and a phylogenetic tree (Kumar, et al.,2019) is constructed by utilizing a neighbor-join method in MEGA version X software.
The full length of the MM7 ITS sequence obtained by sequencing is 586bp, and the sequence is submitted to a GenBank database after proofreading. In the phylogenetic tree based on the Neighbour-Joining algorithm, the strain MM7 ITS and Trichoderma asperellum BFyAs3 form an independent branch in the phylogenetic tree, with the closest relationship (FIG. 2). Combining the morphological characteristic results, the strain MM7 is preliminarily identified to be Trichoderma asperellum (Trichoderma asperellum) and is named as Trichoderma asperellum (Trichoderma asperellum) MM 7.
3.2 Predation of Foc4 by Trichoderma asperellum MM7
3.2.1 predation action of Trichoderma asperellum MM7 on Foc4 Primary Screen
The predation effect of trichoderma asperellum MM7 on Foc4 was determined by agar dot inoculation. As a result, as shown in FIG. 3, Trichoderma asperellum MM7 and banana vascular wilt fungus cake were inoculated to both ends of the plate at a distance of 6cm, respectively, and after 3 days, Trichoderma asperellum MM7 was grown by diffusion to pathogenic bacteria; after 7 days, trichoderma asperellum MM7 began to cover the pathogenic bacteria and prey on them; after 14 days, the pathogenic bacteria Foc4 were completely consumed by trichoderma asperellum MM 7.
3.2.2 Aminochoderma asperellum MM7 rescreening the predation effect of Foc4
The predation effect of trichoderma asperellum MM7 on Foc4 was determined by a cross-shaped vergence method. As shown in FIG. 4, Foc4 was inoculated to the plate "cross" at 4 ends, Trichoderma asperellum MM7 was inoculated to the center, and after 14 days, pathogenic bacteria Foc4 at 4 ends were preyed on by Trichoderma asperellum MM 7.
3.3 Observation of predation effect of Trichoderma asperellum MM7 on Foc4 by slide method
As can be seen from FIG. 5, in the CK control, the pathogenic bacteria Foc4 had intact hyphae, and after adding Trichoderma asperellum MM7, at day 3, Trichoderma asperellum MM7 spores gathered and attached to the Foc4 hyphae, and at day 7, after the Foc4 hyphae were preyed with Trichoderma asperellum spores, the pathogenic bacteria filaments appeared to be obviously scarce, broken and dissolved.
3.4 Observation of predation effect of Trichoderma asperellum MM7 on Foc4 by scanning electron microscopy
Observation of the Foc4 treated by Trichoderma asperellum MM7 and the control group Foc4 by scanning electron microscopy revealed that the untreated Foc4 hyphae had intact and normal morphology, linear structure and smooth cell walls (FIG. 6 a). Foc4 after being treated by trichoderma asperellum MM7, trichoderma asperellum MM7 spores are enriched around hyphae and climb on the hyphae for predation, so that the hyphae are shrunk, shriveled, broken and the like (figure 6 b). The trichoderma asperellum MM7 is shown to have obvious predation effect on Foc 4.
3.5 Effect of Trichoderma asperellum MM7 on cell structure of Foc4 pathogen
The effect of the ultrastructure of Foc4 hyphae after treatment with trichoderma asperellum MM7MM7 was observed by transmission electron microscopy, as shown in fig. 7. The uncooperative Foc4 germ has clear cell shape, smooth and complete cell wall, uniform thickness, complete organelles, uniform cytoplasm and no damage (FIG. 7 a). After the treatment of trichoderma asperellum MM7, trichoderma asperellum MM7 is gathered around cells, the outer wall of the cell wall is digested in a fibrous manner, the cells can not distinguish organelles, most of the organelles are damaged and hydrolyzed, and the cell tissues are disintegrated and can not survive (figure 7 b); foc4 structural disorder in the germ cells, reduction and concentration of the cell matrix, and obvious vacuolation (FIG. 7 c). The result shows that the trichoderma asperellum MM7 has strong predation effect on Foc4 cells.
3.6 evaluation of prevention of banana vascular wilt by Trichoderma MM7
The control effect of trichoderma MM7 on banana wilt of banana potted seedlings is shown in Table 7. Foc4 the index of disease after 60 days of treatment is 86.54%, the disease is serious, and the bulb is obviously blackened; the disease index of the trichoderma MM7 treated group T is 17.33%, the control effect on banana vascular wilt reaches 79.97%, and the result shows that Foc4 pathogenic bacteria in banana root system soil are predated in trichoderma MM7 colonized soil, so that a good control effect on banana vascular wilt is achieved, and the infection of banana vascular wilt can be inhibited.
TABLE 7 prevention and treatment effects of Trichoderma MM7 on banana vascular wilt
The embodiments of the present invention have been described in detail, but the embodiments are merely examples, and the present invention is not limited to the embodiments described above. Any equivalent modifications and substitutions to those skilled in the art are also within the scope of the present invention. Accordingly, equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered by the present invention.
Claims (6)
1. Trichoderma asperellum, named Trichoderma asperellum MM7, is deposited in China Center for Type Culture Collection (CCTCC) at 30 months 3 and 2021, with the preservation number of CCTCC NO: m2021302.
2. Use of trichoderma asperellum according to claim 1 for predating physiological race # 4 of fusarium oxysporum f.sp.
3. Use of the trichoderma asperellum of claim 1 for the preparation of a biocontrol agent for controlling diseases caused by the number 4 physiological races of banana vascular wilt.
4. Use of the trichoderma asperellum of claim 1 for preparing a preparation for predating No. 4 physiological race hyphae of fusarium oxysporum f.sp.
5. Use of the trichoderma asperellum of claim 1 for preparing a preparation for predating the cells of the physiological race of banana vascular wilt disease No. 4.
6. A biocontrol bacterium preparation capable of preying on physiological race # 4 of banana vascular wilt bacteria, comprising the Trichoderma asperellum of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111570689.8A CN114369538A (en) | 2021-12-21 | 2021-12-21 | Trichoderma asperellum preying on banana fusarium wilt and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111570689.8A CN114369538A (en) | 2021-12-21 | 2021-12-21 | Trichoderma asperellum preying on banana fusarium wilt and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114369538A true CN114369538A (en) | 2022-04-19 |
Family
ID=81140630
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111570689.8A Pending CN114369538A (en) | 2021-12-21 | 2021-12-21 | Trichoderma asperellum preying on banana fusarium wilt and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114369538A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115627247A (en) * | 2022-12-21 | 2023-01-20 | 中国热带农业科学院三亚研究院 | Preparation of composite microbial fertilizer and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103013838A (en) * | 2012-12-14 | 2013-04-03 | 江苏耕耘化学有限公司 | Pasty biocontrol preparation for strawberry greensickness, and preparation method, application and special strain thereof |
| CN106085885A (en) * | 2016-08-05 | 2016-11-09 | 广西壮族自治区农业科学院生物技术研究所 | The Trichoderma asperellum of a kind of antagonism banana blight and application thereof |
| CN110272832A (en) * | 2019-08-05 | 2019-09-24 | 海南大学 | Trichoderma asperellum FJ069 and its application |
| CN111893046A (en) * | 2019-05-06 | 2020-11-06 | 广西壮族自治区农业科学院植物保护研究所 | Trichoderma asperellum TZ312 and application thereof, and volatile metabolite of trichoderma asperellum TZ312 and application thereof |
-
2021
- 2021-12-21 CN CN202111570689.8A patent/CN114369538A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103013838A (en) * | 2012-12-14 | 2013-04-03 | 江苏耕耘化学有限公司 | Pasty biocontrol preparation for strawberry greensickness, and preparation method, application and special strain thereof |
| WO2014089951A1 (en) * | 2012-12-14 | 2014-06-19 | 江苏耕耘化学有限公司 | Trichoderma asperellum strain gy20 and paste preparation for preventing and treating fusarium oxysporum schl. f. sp. fragariae |
| CN106085885A (en) * | 2016-08-05 | 2016-11-09 | 广西壮族自治区农业科学院生物技术研究所 | The Trichoderma asperellum of a kind of antagonism banana blight and application thereof |
| CN111893046A (en) * | 2019-05-06 | 2020-11-06 | 广西壮族自治区农业科学院植物保护研究所 | Trichoderma asperellum TZ312 and application thereof, and volatile metabolite of trichoderma asperellum TZ312 and application thereof |
| CN110272832A (en) * | 2019-08-05 | 2019-09-24 | 海南大学 | Trichoderma asperellum FJ069 and its application |
Non-Patent Citations (2)
| Title |
|---|
| 覃柳燕;郭成林;黄素梅;李朝生;韦莉萍;韦绍龙;田丹丹;周维;: "棘孢木霉菌株PZ6对香蕉促生效应及枯萎病室内防效的影响" * |
| 黄远迪;阮云泽;刘铜;高伟;洪珊;丁凯旋;吴明锋;张银东;: "一株棘孢木霉生防效果及固体发酵条件探索" * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115627247A (en) * | 2022-12-21 | 2023-01-20 | 中国热带农业科学院三亚研究院 | Preparation of composite microbial fertilizer and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Guerrero-Molina et al. | More than rhizosphere colonization of strawberry plants by Azospirillum brasilense | |
| CN108102929B (en) | Isaria javanica for resisting pymetrozine and application thereof | |
| CN103160442A (en) | Paecilomyceslilacinus strain having strong pathogenicity for diaphorina citri | |
| CN113025505B (en) | Metarhizium lepigone and biological control method and application thereof in pupal stage of Spodoptera frugiperda | |
| CN115537358A (en) | Bacillus belgii YFB3-1 and separation, screening and identification method and application thereof | |
| CN113444651A (en) | Saffron endophytic fungus and application thereof in preventing and treating bulb rot | |
| CN114032182B (en) | Fungus with functions of antagonizing pathogenic bacteria of garlic root rot and promoting growth | |
| Shadmani et al. | Biocontrol activity of endophytic fungus of barley, Microdochium bolleyi, against Gaeumannomyces graminis var. tritici | |
| CN111893046A (en) | Trichoderma asperellum TZ312 and application thereof, and volatile metabolite of trichoderma asperellum TZ312 and application thereof | |
| CN114369538A (en) | Trichoderma asperellum preying on banana fusarium wilt and application thereof | |
| CN113528395A (en) | Myxococcus xanthus preying on tomato ralstonia solanacearum and application of myxococcus xanthus in biological prevention and control of tomato bacterial wilt | |
| CN101597574A (en) | One Paecilomyces cateniannulatus strain with high yield of spores and screening thereof and application method | |
| CN111778174A (en) | Bacillus subtilis with inhibiting effect on citrus sand skin disease and screening method thereof | |
| CN106591153B (en) | One plant of Metarhizium Strains and its application to carpocapsa pononella highly pathogenicity | |
| CN106282029B (en) | The trichoderma harzianum strain Th-N5 of one plant of resisting carbendazim and its application | |
| CN115927037A (en) | Brevundimonas BWT19-19 and application thereof in preventing and treating plant root-knot nematodes | |
| CN111454848B (en) | Actinomucor elegans GD48, fermentation product, microbial inoculum and application thereof | |
| CN111004727B (en) | Endophytic fungus Z1 for increasing biomass of casuarina equisetifolia in high-salt environment | |
| CN116590152B (en) | Fusarium brick and application thereof | |
| CN114409474A (en) | Trichoderma asperellum bio-organic fertilizer for efficiently promoting crop growth and application thereof | |
| CN116622523B (en) | Blue fungus 401 and application thereof | |
| CN114717139B (en) | Oncorhynchus bacteria strain TP-13 with capacity of promoting growth of new dendrobium roots and application thereof | |
| CN112646733B (en) | Tamarix chinensis endophytic antagonistic fungus as well as separation method and application thereof | |
| CN116716190B (en) | Chaetomium spirochaeta 201 and application thereof | |
| Wu | Using Burkholderia sp. as a biocontrol agent in kiwifruit plants against Neofusicoccum parvum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |