CN114365690A - Dendrobium officinale tissue culture separation equipment - Google Patents
Dendrobium officinale tissue culture separation equipment Download PDFInfo
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- CN114365690A CN114365690A CN202111598951.XA CN202111598951A CN114365690A CN 114365690 A CN114365690 A CN 114365690A CN 202111598951 A CN202111598951 A CN 202111598951A CN 114365690 A CN114365690 A CN 114365690A
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- dendrobium officinale
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- 241001076416 Dendrobium tosaense Species 0.000 title claims abstract description 29
- 238000000926 separation method Methods 0.000 title claims abstract description 28
- 239000007788 liquid Substances 0.000 claims description 62
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 40
- 238000003860 storage Methods 0.000 claims description 17
- 239000004744 fabric Substances 0.000 claims description 12
- 229930191978 Gibberellin Natural products 0.000 claims description 10
- 239000003448 gibberellin Substances 0.000 claims description 10
- 239000007921 spray Substances 0.000 claims description 10
- 238000009833 condensation Methods 0.000 claims description 9
- 230000005494 condensation Effects 0.000 claims description 9
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 9
- 230000009471 action Effects 0.000 claims description 6
- 230000007306 turnover Effects 0.000 claims description 6
- 238000004821 distillation Methods 0.000 claims description 5
- 238000005485 electric heating Methods 0.000 claims description 4
- 239000002985 plastic film Substances 0.000 claims description 4
- 229920006255 plastic film Polymers 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 abstract description 4
- 239000001963 growth medium Substances 0.000 description 10
- 238000005507 spraying Methods 0.000 description 7
- 238000005406 washing Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 240000007643 Phytolacca americana Species 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241001523681 Dendrobium Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/003—Cutting apparatus specially adapted for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a dendrobium officinale tissue culture separation device which comprises a device shell, wherein wheel seats are symmetrically arranged on the upper end face of the device shell, driven wheels and driving wheels are sequentially arranged on the two wheel seats, the driven wheels are connected with the driving wheels through a conveying belt, a stepping motor set is arranged on one side of the driving wheels, bottleneck grooves are formed in the stepping motor set at equal intervals, culture bottles are placed in the bottleneck grooves in an inverted mode, a support is fixedly arranged on the upper end face of the device shell, and a waterproof layer acting on the conveying belt is arranged on the upper end face of the support. According to the dendrobium officinale tissue culture separation equipment, innovative structures such as a stepping transportation structure, a bottle pushing device, a divider, a cleaning structure and a distiller are reasonably combined in the whole equipment, seedlings, culture mediums and culture products can be respectively separated and collected, the integrated automation degree is high, the dendrobium officinale tissue culture separation equipment is suitable for different working conditions, and better use prospects are brought.
Description
Technical Field
The invention relates to the technical field of dendrobium tissue culture, in particular to dendrobium officinale tissue culture separation equipment.
Background
Hardening seedlings for 14-21 days before cultivating the tissue culture seedlings of the dendrobium officinale, and specifically taking the steps that the tissue culture seedlings are moved to a seedling hardening room to be gradually adaptive to the natural environment in an open changing environment, the seedlings can be taken out of a bottle for planting when the leaves of the tissue culture seedlings are dark green, a bottle cover is opened before the seedlings are taken out of the bottle, the bottle seedlings are placed in the outdoor air for 2-3 days to be adaptive to the natural temperature and humidity, then the seedlings are washed, the seedlings with culture medium are taken out slightly when the seedlings are washed, the seedlings are placed in a pot for washing, the tissue culture seedlings are washed by tap water, agar mainly needs to be washed off to prevent the agar from mildewing to cause root rot, and then the seedlings are rinsed by clear water; above group banks up and grows seedlings the process and has a plurality of drawbacks, and first washing seedling is manual washing, and work efficiency is low, lacks dry process after the second washing seedling, and this brings the problem to subsequent seedling work of inserting to if the seedling is not in time inserted, can greatly influence the survival rate, culture medium that third group banked up with earth in the bottle can not reuse, has caused the waste of certain degree, has increased the cultivation cost of producer, for this, we provide a dendrobium officinale group banks up with earth splitter.
Disclosure of Invention
The invention mainly aims to provide dendrobium officinale tissue culture separation equipment which can effectively solve the problems in the background technology.
In order to achieve the purpose, the invention adopts the technical scheme that:
a dendrobium officinale tissue culture separation device comprises a device shell, wherein wheel seats are symmetrically arranged on the upper end face of the device shell, driven wheels and driving wheels are sequentially arranged on the two wheel seats, the driven wheels are connected with the driving wheels through a conveying belt, a stepping motor set is arranged on one side of the driving wheels, bottleneck grooves are formed in the stepping motor set at equal intervals, culture bottles are placed in the bottleneck grooves in an inverted mode, a support is fixedly arranged on the upper end face of the device shell, a waterproof layer acting on the conveying belt is arranged on the upper end face of the support, a through groove is formed in the support, a bottle pusher is arranged on the inner side of the wheel seat and above the conveying belt and comprises a fixing seat, lug pieces, a rotary groove, a push rod and a spring, the fixing seat is connected to the wheel seat through the lug pieces, the rotary groove is located at one end portion of the fixing seat, and the push rod is hinged in the rotary groove, the middle part of the push rod is connected with the fixed seat by a spring;
the lower end of the through groove is fixedly connected with a first slide way, a liquid flow groove is formed in the position, close to the side, of the first slide way, two groups of dividers are installed above the liquid flow groove, each divider comprises a water wheel, a connecting bearing, a divider and a poking rod, the water wheel is fixed on the side wall of the first slide way through the connecting bearing, the dividers are uniformly distributed on the wheel surface of the water wheel, three groups of poking rods are fixed on a middle wheel carrier of the water wheel and extend horizontally, a support is arranged in the equipment shell and located obliquely below the first slide way, a filter screen is horizontally installed on the upper end surface of the support, four groups of shower heads are symmetrically distributed right above the filter screen, a first liquid separating joint is communicated with the upper ends of the four groups of shower heads, the first liquid separating joint is located at the tail end of the first branch pipe, and a plurality of groups of spray heads are installed at the left side of the filter screen at equal intervals, one side of each group of spray heads is communicated with a second liquid-separating joint which is positioned at the tail end of the second branch pipe;
a guide net groove is formed in one side, away from the plurality of groups of spray heads, of the filter screen, a liquid guide cavity is formed in the support and below the filter screen, a distiller is installed at the bottom of the liquid guide cavity, a liquid storage groove is formed in the distiller, a distillation heater is arranged at the bottom of the distiller, a flip cover is movably arranged on the upper end face of the distiller, a drainage cover is arranged at the upper end of the flip cover and communicated with the liquid storage groove, a condensation pipe is communicated with one side of the drainage cover, and the tail end of the condensation pipe extends towards the outer side of the support and is located above the water collecting barrel;
the hopper is installed on the right side of support and the one side that is located the direction guild way, the lower extreme intercommunication of hopper is provided with the second slide, dry cloth has been laid to the inside of second slide, be provided with electric heating wire in the dry cloth, be provided with the gibberellin feed box on the up end of second slide, the bottom intercommunication of gibberellin feed box has the leakage pipe and extends to second slide is inside, the lower extreme of leakage pipe is installed and is cleaned the sponge.
Preferably, the conveyer belt moves clockwise in a stepping mode, the caliber of the bottleneck groove is matched with that of the culture bottle, the caliber of the through groove is the same as that of the bottleneck groove, and the waterproof layer is tightly attached to the conveyer belt.
Preferably, the push rod acts on the culture bottles to push the culture bottles to fall to the outer side of the conveyer belt, and a third slide way is arranged on the outer side of the conveyer belt close to the support seat, so that the culture bottles roll and are collected.
Preferably, the liquid flow groove is less than the horizontal plane of first slide, the scribing stretches into in the liquid flow groove, the poker rod level extends in the top of first slide to be the rubber material, the water wheels drive poker rod and rotate, stir the culture seedling of landing.
Preferably, the first branch pipe and the second branch pipe are communicated with the water supply tank, two groups of water pumps with different powers are respectively installed at the positions, located inside the water supply tank, of the first branch pipe and the second branch pipe, the water pump on the first branch pipe is started firstly, the water pump on the second branch pipe is started later, and a time interval exists between the water pumps.
Preferably, the guide net groove is arc-shaped, the bottom of the groove is provided with a net and is communicated with the liquid guide cavity, the flip cover is provided with a hydraulic press which is opened and closed automatically, when the flip cover is in an opening state, the liquid guide cavity can fall into the liquid storage groove, and the condensation pipe is arranged obliquely downwards.
Preferably, the dry cloth occupies half of the length of the second slideway, an electromagnetic control valve is installed on the leakage pipe, the lower end part of the wiping sponge is cut into a plurality of groups of small sponges, and the small sponges are in contact with the culture surface passing through the second slideway.
Preferably, a bundling box is arranged at the lower end part of the second slide way, and the bundling box comprises a plastic film and a rubber band.
The invention provides dendrobium officinale tissue culture separation equipment through improvement, and compared with the prior art, the dendrobium officinale tissue culture separation equipment has the following remarkable improvements and advantages:
(1) through four groups of shower heads and a plurality of groups of spray heads, the seedlings are comprehensively cleaned, and compared with manual cleaning, the automatic cleaning machine is capable of obviously improving the working efficiency.
(2) After the seedlings are cleaned, the seedlings are immediately dried and coated with a little gibberellin, so that the storage time of the seedlings is prolonged, and the subsequent growth and development are facilitated.
(3) For the culture medium used for the first time, the culture medium with low moisture content can be obtained for secondary use by concentrated recovery and sterilization and dehydration by using a distiller, so that the enterprise cost is reduced.
(4) Whole equipment will step-by-step transportation structure, push away the combination that innovation rational in infrastructure such as bottled ware, decollator, washing structure, distiller to make collection that seedling, culture medium, culture can all accomplish each separation, integrated degree of automation is high.
(5) Whole equipment design is simple, convenient operation, and reduction in production cost improves work efficiency, and the effect of use is better for traditional mode.
Drawings
FIG. 1 is a schematic view of the overall structure of a tissue culture and separation device for Dendrobium officinale;
FIG. 2 is a plan view of a transportation structure of the dendrobium officinale tissue culture separation device of the invention;
FIG. 3 is a detailed structure diagram of a bottle pusher of the tissue culture and separation device for Dendrobium officinale;
FIG. 4 is a diagram of the specific connection positions of the first slide way, the bracket and the second slide way of the dendrobium officinale tissue culture separation device of the invention;
FIG. 5 is a detailed structural diagram of a divider of the tissue culture and separation device for Dendrobium officinale according to the present invention;
FIG. 6 is an internal view of the stand structure of the tissue culture and separation device for Dendrobium officinale according to the present invention;
FIG. 7 is an internal view of a second slide way of the tissue culture and separation device for Dendrobium officinale.
In the figure: 1. an equipment housing; 2. a wheel seat; 3. a driven wheel; 4. a driving wheel; 5. a conveyor belt; 6. a stepping motor set; 7. a bottleneck slot; 8. a culture bottle; 9. a support; 10. a waterproof layer; 11. a through groove; 12. a bottle pusher; 13. filtering with a screen; 14. a tab; 15. rotating the groove; 16. a push rod; 17. a spring; 18. a first slideway; 19. a liquid flow tank; 20. a divider; 21. a water wheel; 22. connecting a bearing; 23. scribing; 24. a poke rod; 25. a support; 26. filtering with a screen; 27. showering and washing the hair; 28. a first liquid separating joint; 29. a first branch pipe; 30. a spray head; 31. a second liquid separation joint; 32. a second branch pipe; 33. a water supply tank; 34. a guide net groove; 35. a drainage cavity; 36. a distiller; 37. a liquid storage tank; 38. a distillation heater; 39. a cover is turned; 40. a drainage cover; 41. a condenser tube; 42. a water collection barrel; 43. a hopper; 44. a second slideway; 45. drying the cloth; 46. an electric heating wire; 47. a gibberellin supply tank; 48. a leakage pipe; 49. wiping sponge; 50. and (7) bundling the boxes.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1-7, a tissue culture separation device for dendrobium officinale comprises a device housing 1, wheel bases 2 are symmetrically arranged on the upper end face of the device housing 1, two groups of wheel bases 2 are sequentially provided with a driven wheel 3 and a driving wheel 4, the driven wheel 3 is connected with the driving wheel 4 by a conveyer belt 5, a stepping motor set 6 is arranged on one side of the driving wheel 4, a bottleneck groove 7 is equidistantly arranged on the stepping motor set 6, a culture bottle 8 is inversely placed in the bottleneck groove 7, a support 9 is fixedly arranged on the upper end face of the device housing 1, a waterproof layer 10 acting on the conveyer belt 5 is arranged on the upper end face of the support 9, a through groove 11 is arranged inside the support 9, a bottle pusher 12 is arranged on the inner side of the wheel base 2 and above the conveyer belt 5, the bottle pusher 12 comprises a fixed base 13, a lug 14, a rotary groove 15, a push rod 16 and a spring 17, the fixed base 13 is connected to the wheel bases 2 through the lug 14, the rotary groove 15 is positioned at one end part of the fixed seat 13, a push rod 16 is hinged in the rotary groove 15, and the middle part of the push rod 16 is connected with the fixed seat 13 through a spring 17;
a first slide way 18 is fixedly connected to the lower end of the through groove 11, a liquid flowing groove 19 is formed in the position, close to the side edge, of the upper portion of the first slide way 18, a two-component divider 20 is installed above the liquid flowing groove 19, the divider 20 comprises a water wheel 21, a connecting bearing 22, a scribing sheet 23 and a poking rod 24, the water wheel 21 is fixed to the side wall of the first slide way 18 through the connecting bearing 22, the scribing sheets 23 are uniformly distributed on the wheel surface of the water wheel 21, three groups of poking rods 24 are fixed to a middle wheel frame of the water wheel 21 and extend horizontally, a support 25 is arranged in the equipment shell 1 and located obliquely below the first slide way 18, a filter screen 26 is horizontally installed on the upper end surface of the support 25, four groups of shower heads 27 are symmetrically distributed right above the filter screen 26, the upper ends of the four groups of shower heads 27 are communicated with a first liquid distributing joint 28, the first liquid distributing joint 28 is located at the tail end of a first branch pipe 29, a plurality of groups of shower heads 30 are installed on the left side of the filter screen 26 at equal intervals, one side of each group of spray heads 30 is communicated with a second liquid separating joint 31, and the second liquid separating joint 31 is positioned at the tail end of the second branch pipe 32;
a guide net groove 34 is arranged on one side of the filter screen 26, which is far away from the plurality of groups of spray heads 30, a liquid guide cavity 35 is arranged inside the support 25 and below the filter screen 26, a distiller 36 is arranged at the bottom of the liquid guide cavity 35, a liquid storage groove 37 is arranged inside the distiller 36, a distillation heater 38 is arranged at the bottom of the distiller 36, a turnover cover 39 is movably arranged on the upper end surface of the distiller 36, a drainage cover 40 is arranged at the upper end of the turnover cover 39 and communicated with the liquid storage groove 37, a condensation pipe 41 is communicated with one side of the drainage cover 40, the tail end of the condensation pipe 41 extends towards the outer side of the support 25 and is positioned above a water collecting barrel 42, a sliding door is arranged at the rear end of the equipment shell 1, and the culture medium can be taken out of the liquid storage groove 37 by opening the sliding door and the turnover cover 39;
a hopper 43 is arranged on the right side of the bracket 25 and positioned on one side of the guide net groove 34, the lower end of the hopper 43 is communicated with a second slideway 44, dry cloth 45 is laid in the second slideway 44, an electric heating wire 46 is arranged in the dry cloth 45, a gibberellin liquid supply tank 47 is arranged on the upper end surface of the second slideway 44, the bottom of the gibberellin liquid supply tank 47 is communicated with a leakage pipe 48 and extends into the second slideway 44, a wiping sponge 49 is arranged at the lower end of the leakage pipe 48, and gibberellin liquid can be slowly dropped into the wiping sponge 49 through the leakage pipe 48;
the conveyer belt 5 moves clockwise step by step, the caliber of the bottle mouth groove 7 is matched with that of the culture bottle 8, the caliber of the through groove 11 is the same as that of the bottle mouth groove 7, and the waterproof layer 10 is tightly attached to the conveyer belt 5; the push rod 16 acts on the culture bottles 8 to push the culture bottles 8 to fall to the outer side of the conveyer belt 5, and a third slide way is arranged on the outer side of the conveyer belt 5 close to the support 9 for the culture bottles 8 to roll off and be collected; the liquid flowing groove 19 is lower than the horizontal plane of the first slideway 18, the scribing sheet 23 extends into the liquid flowing groove 19, the poking rod 24 horizontally extends above the first slideway 18 and is made of rubber, and the water wheel 21 drives the poking rod 24 to rotate so as to poke the fallen culture seedlings; the first branch pipe 29 and the second branch pipe 32 are both communicated with the water supply tank 33, two groups of water pumps with different powers are respectively installed at the positions, located inside the water supply tank 33, of the first branch pipe 29 and the second branch pipe 32, the water pumps on the first branch pipe 29 are started firstly, the water pumps on the second branch pipe 32 are started later, and a time interval exists between the water pumps; the guide net groove 34 is arc-shaped, the bottom of the groove is provided with a net and is communicated with the liquid guide cavity 35, the flip cover 39 is provided with a hydraulic press which can be automatically opened and closed, when the flip cover is in an opening state, the liquid guide cavity 35 can fall into the liquid storage groove 37, and the condensation pipe 41 is obliquely arranged downwards; the dry cloth 45 occupies half of the length of the second slideway 44, an electromagnetic control valve is arranged on the leakage pipe 48 and is used for controlling the leakage speed, the lower end part of the wiping sponge 49 is cut into a plurality of groups of small sponges, the contact area and frequency with the seedlings are improved, and the small sponges are in contact with the culture surface passing through the second slideway 44; a strapping box 50 is arranged at the lower end of the second slideway 44, and the strapping box 50 comprises a plastic film and a rubber band.
It should be noted that, the invention is a dendrobium officinale tissue culture separation device, when in use, a worker quickly puts a culture bottle 8 upside down on a bottle mouth groove 7 of a conveyor belt 5, along with the stepping movement of the conveyor belt 5, because the culture bottle 8 is kept in contact with a waterproof layer 10, the liquid in the culture bottle 8 cannot leak downwards, when the culture bottle 8 moves to the upper end of a through groove 11, the culture bottle 8 is just in a stepping gap, at the moment, the liquid culture medium and a plurality of seedlings in the culture bottle 8 fall into a first slideway 18 from the through groove 11 because of gravity, after the culture bottle 8 releases objects, the culture bottle 8 continues to move along with the conveyor belt 5, the culture bottle contacts a push rod 16 of a bottle pusher 12 forwards, the push rod 16 rotates, along with the increase of the acting force of a spring 17, the push rod 16 moves reversely, pushes the culture bottle 8 upside down, rolls to a third slideway on one side of the conveyor belt 5, slides downwards to be collected, and the liquid and the seedlings slide downwards in the first slideway 18, the liquid enters the liquid flowing groove 19 along with sliding, acts on the scribing sheet 23 when flowing, enables the water wheel 21 to rotate, stirs the fallen culture seedlings, enables a plurality of tissue culture seedlings which are originally agglomerated to be separated into single seedlings and fall one by one, is convenient for subsequent cleaning, then the seedlings are suspended on the filter screen 26, the culture medium liquid leaks downwards to enter the liquid guide cavity 35 and then enter the liquid storage groove 37 for temporary storage, at the moment, the seedlings are cleaned, four groups of spraying heads 27 are used for fully spraying to cover the whole filter screen 26, a plurality of groups of spraying heads 30 are used for spraying after spraying, the spraying speed is high, on one hand, the spraying effect is improved, on the other hand, a plurality of seedlings are directly pushed to the edge of the filter screen 26, are guided to fall through the guide net groove 34, enter the hopper 43 for transition, then enter the second slideway 44, when the seedlings roll in the slideway, the seedlings first contact with a section of dry cloth 45, the dry cloth 45 has a drying effect, when the seedlings are wiped at the sponge 49, stems and leaves of the seedlings touch small sponges, the small sponges transfer gibberellins on the surfaces of the small sponges to the stems and leaves of the seedlings, the storage time of the seedlings is prolonged, subsequent growth and development are facilitated, the seedlings intensively enter the bundling box 50, are bundled by plastic films after being wrapped, and are ready to use, the liquid storage tank 37 stores a large amount of culture media, moisture and bacteria, the turnover cover 39 is closed at the moment, the distillation heater 38 is started, the temperature in the liquid storage tank 37 is raised to 110 ℃, on one hand, high-temperature sterilization is carried out, on the other hand, moisture can be evaporated, the obtained low-moisture culture media can be used for the second time, and the evaporated moisture passes through the drainage cover 40 and the condenser pipe 41 and is collected by the water collection barrel 42.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides a dendrobium officinale tissue culture splitter, includes equipment shell (1), its characterized in that: the up end symmetry of equipment shell (1) is provided with wheel seat (2), install in proper order on two sets of wheel seats (2) from driving wheel (3) and action wheel (4), utilize transportation area (5) to connect from driving wheel (3) and action wheel (4), step motor group (6) are installed to one side of action wheel (4), bottleneck groove (7) have been seted up on step motor group (6) equidistance, place upside down in bottleneck groove (7) and put into blake bottle (8), the fixed support (9) that is provided with of up end of equipment shell (1), the up end of support (9) is provided with waterproof layer (10) that act on transportation area (5), logical groove (11) have been seted up to the inside of support (9), the inboard of wheel seat (2) is provided with above being located transportation area (5) and pushes away bottled ware (12), it includes fixing base (13) to push away bottled ware (12), The wheel seat is characterized by comprising a lug plate (14), a rotary groove (15), a push rod (16) and a spring (17), wherein the fixed seat (13) is connected to the wheel seat (2) through the lug plate (14), the rotary groove (15) is positioned at one end part of the fixed seat (13), the push rod (16) is hinged in the rotary groove (15), and the middle part of the push rod (16) is connected with the fixed seat (13) through the spring (17);
the lower end of the through groove (11) is fixedly connected with a first slide way (18), a liquid flowing groove (19) is formed in the position, close to the side, of the upper portion of the first slide way (18), two groups of dividers (20) are installed above the liquid flowing groove (19), each divider (20) comprises a water wheel (21), a connecting bearing (22), scribing sheets (23) and a poking rod (24), the water wheels (21) are fixed on the side wall of the first slide way (18) through the connecting bearings (22), the scribing sheets (23) are uniformly distributed on the wheel surface of the water wheels (21), three groups of poking rods (24) are fixed on a wheel carrier in the middle of the water wheels (21) and extend horizontally, a support (25) is arranged in the equipment shell (1) and located below the first slide way (18) obliquely, a filter screen (26) is horizontally installed on the upper end surface of the support (25), and four groups of shower heads (27) are symmetrically distributed right above the filter screen (26), the upper ends of the four groups of shower heads (27) are communicated with a first liquid separating joint (28), the first liquid separating joint (28) is positioned at the tail end of a first branch pipe (29), a plurality of groups of spray heads (30) are arranged at the left side of the filter screen (26) at equal intervals, one side of each group of spray heads (30) is communicated with a second liquid separating joint (31), and the second liquid separating joint (31) is positioned at the tail end of a second branch pipe (32);
a guide net groove (34) is formed in one side, away from the plurality of groups of spray heads (30), of the filter screen (26), a liquid guide cavity (35) is formed in the support (25) and located below the filter screen (26), a distiller (36) is installed at the bottom of the liquid guide cavity (35), a liquid storage groove (37) is formed in the distiller (36), a distillation heater (38) is arranged at the bottom of the distiller (36), a turnover cover (39) is movably arranged on the upper end face of the distiller (36), a drainage cover (40) is arranged at the upper end of the turnover cover (39) and communicated with the liquid storage groove (37), a condensation pipe (41) is communicated with one side of the drainage cover (40), and the tail end of the condensation pipe (41) extends towards the outer side of the support (25) and is located above a water collection barrel (42);
hopper (43) is installed on the right side of support (25) and the one side that is located direction guild way (34), the lower extreme intercommunication of hopper (43) is provided with second slide (44), dry cloth (45) have been laid to the inside of second slide (44), be provided with electric heating wire (46) in dry cloth (45), be provided with gibberellin on the up end of second slide (44) and supply liquid case (47), the bottom intercommunication of gibberellin supply liquid case (47) has leakage pipe (48) and extends to second slide (44) inside, the lower extreme of leakage pipe (48) is installed and is cleaned sponge (49).
2. The tissue culture and separation device for dendrobium officinale according to claim 1, which is characterized in that: the conveyer belt (5) moves clockwise in a stepping mode, the caliber of the bottleneck groove (7) is matched with that of the culture bottle (8), the caliber of the through groove (11) is the same as that of the bottleneck groove (7), and the waterproof layer (10) is tightly attached to the conveyer belt (5).
3. The tissue culture and separation device for dendrobium officinale according to claim 1, which is characterized in that: the push rod (16) acts on the culture bottles (8) to push the culture bottles (8) to fall to the outer side of the conveyer belt (5), and a third slide way is arranged at the position, close to the support (9), of the outer side of the conveyer belt (5) and used for enabling the culture bottles (8) to roll off and be collected.
4. The tissue culture and separation device for dendrobium officinale according to claim 1, which is characterized in that: liquid flowing groove (19) are less than the horizontal plane of first slide (18), in scribing (23) stretched into liquid flowing groove (19), poker rod (24) level extension is in the top of first slide (18) to be the rubber material, water wheels (21) drive poker rod (24) and rotate, stir the cultivation seedling of landing.
5. The tissue culture and separation device for dendrobium officinale according to claim 1, which is characterized in that: the first branch pipe (29) and the second branch pipe (32) are communicated with the water supply tank (33), two groups of water pumps with different powers are respectively installed at positions, located inside the water supply tank (33), of the first branch pipe (29) and the second branch pipe (32), the water pumps on the first branch pipe (29) are started firstly, the second branch pipe (32) is started later, and time intervals exist between the first branch pipe and the second branch pipe.
6. The tissue culture and separation device for dendrobium officinale according to claim 1, which is characterized in that: the guide net groove (34) is arc-shaped, a net is arranged at the bottom of the groove and is communicated with the liquid guide cavity (35), a hydraulic press is arranged on the flip cover (39) and is opened and closed automatically, when the flip cover is in an opening state, the liquid guide cavity (35) can fall into the liquid storage groove (37), and the condensation pipe (41) is arranged obliquely downwards.
7. The tissue culture and separation device for dendrobium officinale according to claim 1, which is characterized in that: the dry cloth (45) occupies half of the length of the second slideway (44), an electromagnetic control valve is installed on the leakage pipe (48), the lower end part of the wiping sponge (49) is cut into a plurality of groups of small sponges, and the small sponges are in contact with the culture surface passing through the second slideway (44).
8. The tissue culture and separation device for dendrobium officinale according to claim 1, which is characterized in that: a bundling box (50) is arranged at the lower end part of the second slide way (44), and a plastic film and a rubber band are arranged in the bundling box (50).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111598951.XA CN114365690A (en) | 2021-12-24 | 2021-12-24 | Dendrobium officinale tissue culture separation equipment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111598951.XA CN114365690A (en) | 2021-12-24 | 2021-12-24 | Dendrobium officinale tissue culture separation equipment |
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| CN114365690A true CN114365690A (en) | 2022-04-19 |
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| CN202111598951.XA Pending CN114365690A (en) | 2021-12-24 | 2021-12-24 | Dendrobium officinale tissue culture separation equipment |
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| CN107581064A (en) * | 2017-08-18 | 2018-01-16 | 福建连城兰花股份有限公司 | A kind of orchid inoculation method |
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