CN114354793A - Method for constructing high performance liquid fingerprint sugar spectrum of polygonatum sibiricum decoction pieces prepared by steaming at different times - Google Patents
Method for constructing high performance liquid fingerprint sugar spectrum of polygonatum sibiricum decoction pieces prepared by steaming at different times Download PDFInfo
- Publication number
- CN114354793A CN114354793A CN202111645269.1A CN202111645269A CN114354793A CN 114354793 A CN114354793 A CN 114354793A CN 202111645269 A CN202111645269 A CN 202111645269A CN 114354793 A CN114354793 A CN 114354793A
- Authority
- CN
- China
- Prior art keywords
- fingerprint
- decoction pieces
- steaming
- polysaccharide
- rhizoma polygonati
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a method for constructing a high performance liquid fingerprint chromatogram sugar spectrum of polygonatum sibiricum decoction pieces steamed for different times. Constructing fingerprint spectrums of polygonatum sibiricum decoction pieces of the wine with different steaming times by adopting a high performance liquid chromatography and a traditional Chinese medicine fingerprint spectrum similarity evaluation system; determining that the fingerprint spectrums of polygonatum sibiricum decoction pieces subjected to different steaming times contain 8 common characteristic monosaccharide components which are mannose (Man), glucosamine hydrochloride (GluN), ribose (Rib), rhamnose (Rha), galacturonic acid (GalA), glucose (Glu), galactose (Gal) and arabinose (Arab); the method has the characteristics of small sample dosage, strong characteristics and reliable result, and is convenient for popularization and application in the processing direction of the traditional Chinese medicinal materials.
Description
Technical Field
The invention belongs to the field of detection of processing active ingredients of traditional Chinese medicine decoction pieces, and particularly relates to a method for constructing a high-efficiency liquid-phase fingerprint sugar spectrum of rhizoma polygonati preparata decoction pieces with different steaming times.
Background
Rhizoma Polygonati (Polygonatum sibiricum Red.) is a herb of genus Polygonatum of family Liliaceae, and is mainly distributed in northeast region, and is divided into rhizoma Polygonati, Polygonatum kingianum and Polygonatum cyrtonema Hua. It is sweet in taste and neutral in nature, and has the functions of invigorating qi, nourishing yin, invigorating spleen, invigorating kidney and moistening lung. However, the raw polygonatum rhizome is numb and irritating in mouth and tongue when taken, so the effect can be enhanced by adding the raw polygonatum rhizome after being processed, and the rhizoma polygonati is mostly steamed. Rhizoma Polygonati is used as a medicine and food homologous Chinese medicine, and its chemical components mainly comprise polysaccharide, saponin, flavone, lignan, amino acid, trace elements, volatile oil, etc. Wherein, the polygonatum polysaccharide is the main component of the medicine, and has the pharmacological actions of reducing blood sugar, reducing blood fat and the like. Polysaccharide is the only quality control component of rhizoma Polygonati specified in 2020 edition of Chinese pharmacopoeia, but the change research of processed rhizoma Polygonati polysaccharide is shallow.
Under the background of increasing importance of traditional Chinese medicines, the traditional Chinese medicines growing in wild cannot meet the needs of the market, and the problem that the chemical components of the traditional Chinese medicines are influenced by factors such as growing environment, transportation, storage, processing and the like cannot be solved through artificial cultivation, so that quality monitoring becomes an important research subject.
The traditional Chinese medicine fingerprint spectrum technology is one of the most effective, accurate and direct methods for quality control of traditional Chinese medicines acknowledged at home and abroad at present. The method provides more information, reflects the types and contents of chemical components more comprehensively, reflects the overall effect of the traditional Chinese medicine components to evaluate the quality of the traditional Chinese medicine, and is not only applied to the quality control of the traditional Chinese medicine, but also widely applied to the aspects of basic research of pharmacodynamic substances of the traditional Chinese medicine and the like.
The emphasis in the study of the quality of polysaccharides is on monosaccharide composition, molecular weight, configuration, etc. The molecular structure change of the processed polygonatum polysaccharide determines the quality and curative effect of the polygonatum. The pharmacological action of the polysaccharide can be better utilized only by fully understanding the structural characteristics of the polysaccharide, so that the construction of the fingerprint sugar spectrum of the polysaccharide of the polygonatum sibiricum processed decoction pieces has important significance for the control of the processing of the tablets.
Disclosure of Invention
Based on the background technology, the invention mainly aims to provide a high-efficiency liquid-phase fingerprint sugar spectrum construction method for polygonatum sibiricum decoction pieces subjected to steaming for different times, which can show polysaccharide characteristics of raw products, primary steaming, secondary steaming and tertiary steaming tablets and provide a basis for processing control and drug effect of traditional Chinese medicine decoction pieces.
The purpose of the invention can be realized by the following technical scheme:
(1) repeatedly steaming crude rhizoma Polygonati decoction pieces for three times, adding 20kg yellow wine into every 100kg rhizoma Polygonati decoction pieces, adding appropriate amount of water into yellow wine to make rhizoma Polygonati be completely and uniformly stirred, wherein the ratio of wine to water is 1: 1.875 (batch mixing), moistening for 80min, steaming for 6 h, drying at 70 ℃ for 13 h to obtain rhizoma polygonati decoction pieces, and repeating the steps to obtain rhizoma polygonati decoction pieces subjected to primary steaming and tertiary steaming.
(2) Pulverizing the processed decoction pieces, sieving with 60 mesh sieve, extracting twice at 75 deg.C for 2 hr by hot water reflux method according to material-liquid ratio of 1:15 g/ml, concentrating the extractive solution, freeze drying to obtain solid powder crude polysaccharide, and purifying with cotton-like DEAE cellulose.
(3) 1g of crude polysaccharide was prepared as a 16.7mg/ml solution. Weighing cotton-shaped DEAE cellulose, performing ultrasonic defoaming, loading on a column, and adsorbing for 24h according to a proportion (crude polysaccharide: the drained cotton-shaped DEAE =1:70 (g/g)). Adjusting flow rate to 1mg/ml, eluting with distilled water to obtain water-washed purified polysaccharide, and eluting with high concentration (0.5 mol/l) NaCl to obtain salt-washed purified polysaccharide (the salt-washed eluate needs dialysis for 24 hr to remove pigment).
(4) The monosaccharide components were determined by PMP pre-column derivatization. Chromatographic conditions are as follows: a Thermo Ultimate3000 High Performance Liquid Chromatograph (HPLC) was used in combination with a Thermo U3000 Diode Array Detector (DAD) and a Supersil ODS2 column (5 μm, 4.6 mm. times.250 mm). Sample introduction amount: 20 mu L of the solution; mobile phase: PBS (pH = 6.8) and acetonitrile (82: 18 v/v); a flow rate; 0.8 mL/min; column temperature: c, 30 ℃; detector wavelength 245 nm; the running time is as follows: and 85 min.
(5) HPLC fingerprint data of rhizoma Polygonati polysaccharide is processed by software of Chinese medicinal chromatogram fingerprint similarity evaluation system 2004A version, and reference fingerprint is generated by using S1 sample fingerprint as reference spectrum and time window width of 0.1 and using median method. In total, 8 common peaks were identified, Man, GluN, Rib, Rha, GalA, Glu, Gal, Arab. The similarity analysis can obtain that the similarity of the polysaccharide is more than 0.9, and the requirement of the similarity of the chromatographic fingerprint of the traditional Chinese medicine is met.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the invention firstly analyzes the polygonatum sibiricum decoction piece polysaccharide with different steaming times, constructs monosaccharide composition fingerprint spectra, determines 8 common characteristic monosaccharide peaks, has similarity of more than 0.9, meets the requirement of the similarity of the traditional Chinese medicine chromatogram fingerprint spectra, and provides basis for controlling the processing medicinal components of polygonatum sibiricum decoction pieces.
FIG. 1 is a comparison fingerprint (median method) (peaks 1-8 are common peaks for each batch); FIG. 2 is HPLC finger print of polysaccharide samples of decoction pieces with different steaming times.
Detailed Description
The present invention is further illustrated by the following examples, which are meant to be illustrative of the present invention and are not to be construed as limiting thereof.
Example 1 processing of rhizoma Polygonati decoction pieces with wine
Referring to the processing technology disclosed in CN113616729A, 20kg of yellow wine is added into every 100kg of rhizoma polygonati decoction pieces, and a proper amount of water is added into the yellow wine to ensure that the rhizoma polygonati can be completely and uniformly stirred, wherein the ratio of the wine to the water is 1: 1.875 (batch mixing), moistening for 80min, steaming for 6 h, drying at 70 ℃ for 13 h to obtain rhizoma polygonati decoction pieces, and repeating the steps to obtain rhizoma polygonati decoction pieces subjected to primary steaming and tertiary steaming.
Example 2 extraction and purification of polysaccharides
1. Extraction of
Pulverizing rhizoma Polygonati decoction pieces, and sieving with 60 mesh sieve. Extracting twice by a hot water reflux method according to a feed-liquid ratio of 1:15 (g/ml), an extraction temperature of 75 ℃, an extraction time of 2 h. The combined filtrates were concentrated and lyophilized to give a crude polysaccharide powder for further purification.
2. Purification of
Cotton-like DEAE cellulose pretreatment: soaking cotton-shaped DEAE cellulose in distilled water for 24h to fully swell the cellulose, and performing suction filtration by using a Buchner funnel; soaking the mixture for 2 to 3 hours by using 0.5mol/L NaOH solution, carrying out suction filtration, and repeatedly washing the mixture by using distilled water until the pH value is about 7; soaking the mixture for 2 to 3 hours by using 0.5mol/L HCl solution, repeatedly washing the mixture by using distilled water until the pH value is about 7, and draining the mixture for later use.
Column mounting: 1g of crude polysaccharide was prepared as a 16.7mg/ml solution. Weighing a certain amount of treated cotton-shaped DEAE cellulose, performing ultrasonic debubbling, loading on a column, and proportionally loading (rhizoma Polygonati crude polysaccharide: drained cotton-shaped DEAE =1:70 (g/g)). And a dropper is used for slowly loading the sample around the column to prevent the sugar from shaking greatly and adsorbing for 24 hours. Adjusting the column to flow rate of 1mg/mL, eluting with distilled water until no color is developed after detection by phenol-sulfuric acid method; namely, the solution is eluted by NaCl with high concentration (0.5 mol/L) until no color is developed when the solution is detected by a phenol-sulfuric acid method. Collecting the eluent, and concentrating the eluents washed by water and salt respectively. After concentration, directly carrying out freeze drying on the eluent washed by water; dialyzing the eluent subjected to salt washing for 24h to remove pigment, and freeze-drying to obtain purified water-washed polysaccharide and salt-washed polysaccharide powder, wherein the yield of water washing and salt washing of the polygonatum polysaccharide with different steaming times is shown in table 1.
TABLE 1 yield of rhizoma Polygonati polysaccharide water washing and salt washing with different times of steaming
EXAMPLE 2 determination of monosaccharide composition
And (3) drawing a monosaccharide linear regression equation: accurately weighing Man 6mg, Rib 5mg, Rha 10mg, GluA 5mg, GalA 10mg, Glu 10mg, Gal 10mg, Xyl 4mg,Mixing Arab 10mg, Fuc 10mg and GluN 6mg, and diluting to 10 mL with 60% acetonitrile as solvent to obtain 11 monosaccharide mixed standard solutions. Respectively taking 1 mL of mixed standard solution, diluting 3 times, 6 times, 10 times, 12 times and 24 times by taking 60% acetonitrile as a diluting solvent to obtain 11 monosaccharide mixed standard solutions with a certain concentration gradient, and performing linear regression by taking a logarithm value (logc) of monosaccharide concentration (c) as a horizontal coordinate and a logarithm value (logS) of a peak area (S) as a vertical coordinate to obtain a linear regression equation of the 11 monosaccharides. In addition, the mixed standard solution is continuously diluted according to the actual detection result until the signal-to-noise ratio (S/N) of the signal peak of each monosaccharide is detected to be not less than 10 (namely, the limit of quantitation) and the signal-to-noise ratio (S/N) is not less than 3 (namely, the limit of detection). Precision measurement, 11 monosaccharide mixed standard solutions, repeat sample injection 6 times to draw monosaccharide standard curve, see table 2, correlation coefficient R2All are above 0.99, which shows that the matching degree of each concentration of monosaccharide components and peak area is good, and the monosaccharide components have better linear relation.
Sample pretreatment and determination: respectively and precisely weighing 5mg of polysaccharide, adding 2 mL of 2 mol/L trifluoroacetic acid (TFA), putting into a hydrothermal reactor, and hydrolyzing for 6 h at 110 ℃. After this time, the hydrolysate was evaporated thoroughly and after further drying, the resulting powder sample was redissolved with 95% ethanol and the re-evaporation process of the powder sample was repeated at least four times to remove any residual TFA. Dissolving in 60% acetonitrile, centrifuging to obtain supernatant, filtering with 0.45 μm microporous membrane, sampling, detecting to obtain separated peak area, substituting into linear regression equation, calculating the mass concentration of each monosaccharide, and calculating the molar ratio of monosaccharide components.
Chromatographic conditions are as follows: the liquid chromatography of monosaccharide adopts high performance liquid chromatography combined with evaporation light detector (HPLC-ELSD), and the chromatographic column adopts Supersil NH2-S5 μm (4.6 mm × 250 mm) column, hplc using a siemer fly Ultimate3000, recording and data processing using Chromeleon 7.2 software, dry air as carrier gas, sample size 20 μ L. The chromatographic conditions were set as follows: the mobile phase is acetonitrile and water (75: 25 v/v); the flow rate is 1.0 mL/min; the atomization temperature is 35 ℃; the column temperature is 30 ℃; the evaporation temperature is 45 ℃; run time 40min; a gain of 1; the gas pressure was 27 psi.
TABLE 211 Standard Curve for the monosaccharide standards
As can be seen from Table 3, the peak emergence times of the 11 standards are Man (15.96 min), GluN (18.559 min), Rib (20.92 min), Rha (22.48 min), GluA (24.38 min), GalA (27.17 min), Glu (32.05 min), Gal (36.10 min), Xyl (39.01 min), Arab (40.15 min) and Fuc (47.45 min) in this order. And (4) taking the peak area of the sample into a monosaccharide standard curve, and calculating the monosaccharide molar ratio.
TABLE 3 mean retention time of standards
TABLE 4 monosaccharide composition and molar ratio of crude product, rhizoma Polygonati decoction pieces polysaccharide obtained by steaming one, steaming two and steaming three
As can be seen from Table 4, the polysaccharide of the decoction pieces of Polygonati officinalis rhizoma is mainly composed of Glue, Fuc, Arab and Gal. The polysaccharide content of the raw product purified water washing is Glu, and Fuc and Gal are the most; the most abundant salt washes were Fuc followed by Glu and Gal. Compared with other groups, the polysaccharide washed by steaming, purifying and washing only contains 9 kinds of monosaccharides, and mainly contains Glu, Gal and Fuc; the content of monosaccharides is generally increased in salt washing compared with other groups, and the proportion of Gal is the largest. The polysaccharide of the secondary evaporation, purification and water washing accounts for Gal at the most, and Glu and GalA are obtained; the highest content of salt washes is Gal followed by Glu. The polysaccharide washed by the three-steaming purification water has the highest Glu content, and then Gal and Man are added; the highest content of salt washes is Gal. The content of GluN in rhizoma Polygonati decoction pieces polysaccharide is minimum.
Example 2 establishment of HPLC finger print of rhizoma Polygonati decoction piece polysaccharide with different steaming times
1. Fingerprint spectrum establishment result
The HPLC fingerprint data of the polygonatum polysaccharide is subjected to data processing by software of a traditional Chinese medicine chromatogram fingerprint similarity evaluation system 2004A version, the fingerprint of an S1 sample is taken as a reference spectrum, the time window width is 0.1, the reference fingerprint is generated by a median method through multipoint correction and automatic matching, and the reference fingerprint is shown in figure 1. The fingerprint map determines 8 common peaks which are Man, GluN, Rib, Rha, GalA, Glu, Gal and Arab. Raw product water washing, salt washing, first steam washing, salt washing, second steam washing, salt washing, third steam washing, and salt washing to obtain HPLC fingerprint shown in figure 2.
2. Peak area of common fingerprint peak
According to the table 5, under the chromatographic condition, all characteristic peaks of the polysaccharide can be well separated, the retention time is stable, and the common peaks can well appear, and meanwhile, the common peak area of each spectrogram accounts for more than 80% of the total peak area, which indicates that the chemical components of the polygonatum polysaccharide with different steaming times are not greatly different, and the fingerprint accords with the evaluation requirement of the fingerprint, and the next spectral efficiency relation analysis can be carried out.
TABLE 5 common peak matching data table of rhizoma Polygonati polysaccharide finger print
TABLE 6 common peak matching data table of rhizoma Polygonati polysaccharide fingerprint (one continuation)
TABLE 7 common peak matching data table of rhizoma Polygonati polysaccharide fingerprint (second)
3. Calculation of similarity
The similarity analysis is shown in table 8, and the similarity of the polygonatum polysaccharide is more than 0.9, which meets the requirement of the similarity of the chromatographic fingerprint of the traditional Chinese medicine.
Table 8 similarity results
TABLE 9 results of similarity (continue table)
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.
Claims (6)
1. A high performance liquid phase fingerprint sugar spectrum construction method of polygonatum sibiricum decoction pieces with different steaming times is characterized by comprising the following steps: repeatedly steaming rhizoma Polygonati crude decoction pieces for three times to obtain first-steamed, second-steamed and third-steamed rhizoma Polygonati decoction pieces; extracting and purifying polysaccharide from crude decoction pieces, the first-steaming, the second-steaming and the third-steaming decoction pieces; and (3) carrying out HPLC (high performance liquid chromatography) on the purified polysaccharide sample to determine monosaccharide composition, and establishing a fingerprint of a high performance liquid chromatograph.
2. The method of claim 1, wherein: adding 20kg of yellow wine into every 100kg of rhizoma polygonati decoction pieces, and mixing the materials in the yellow wine according to the wine-water ratio of 1: 1.875 Water is added to stir the rhizoma polygonati completely and evenly, the mixture is moistened for 80min, the steaming time is 6 h, the drying temperature is 70 ℃, and the drying time is 13 h, so that the rhizoma polygonati decoction pieces which are steamed once are obtained, and the steps are repeated to obtain the rhizoma polygonati decoction pieces which are steamed twice and steamed three times.
3. The method of claim 1, wherein: pulverizing the processed decoction pieces, sieving with 60 mesh sieve, extracting with hot water under reflux at 75 deg.C for 2 hr twice at a ratio of 1:15 g/ml, concentrating the extractive solution, and freeze drying to obtain solid powder of crude polysaccharide.
4. The method of claim 1, wherein: adsorbing the sample solution with cotton-like DEAE cellulose, eluting with distilled water to obtain water-washed purified polysaccharide, eluting with 0.5mol/mL NaCl to obtain salt-washed purified polysaccharide, and dialyzing the salt-washed eluate for 24 hr to remove pigment.
5. The method of claim 1, wherein: measuring monosaccharide components by adopting a PMP pre-column derivatization method, wherein the chromatographic conditions are as follows: thermo Ultimate3000 High Performance Liquid Chromatography (HPLC) was used in combination with Thermo U3000 Diode Array Detector (DAD), Supersil ODS2 column (5 μm, 4.6 mm × 250 mm), sample size: 20 mu L of the solution; mobile phase: PBS (pH = 6.8) and acetonitrile (82: 18 v/v); a flow rate; 0.8 mL/min; column temperature: c, 30 ℃; detector wavelength 245 nm; the running time is as follows: 85 min, 11 monosaccharides mannose (Man), ribose (Rib), rhamnose (Rha), glucuronic acid (GluA), galacturonic acid (GalA), glucose (Glu), galactose (Gal), xylose (Xyl), arabinose (Arab), fucose (Fuc) and glucosamine hydrochloride (GluN) as standard monosaccharides.
6. The method of claim 5, wherein: performing data processing on HPLC fingerprint data of polygonatum polysaccharides by using software of a traditional Chinese medicine chromatogram fingerprint similarity evaluation system 2004A version, taking an S1 sample fingerprint as a reference spectrum, setting the time window width to be 0.1, performing multi-point correction, performing automatic matching, and generating a control fingerprint by using a median method, wherein the fingerprint determines 8 common peaks respectively including Man, GluN, Rib, Rha, GalA, Glu, Gal and Arab; the similarity analysis can obtain that the similarity of the polysaccharide is more than 0.9, and the requirement of the similarity of the chromatographic fingerprint of the traditional Chinese medicine is met.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111645269.1A CN114354793A (en) | 2021-12-30 | 2021-12-30 | Method for constructing high performance liquid fingerprint sugar spectrum of polygonatum sibiricum decoction pieces prepared by steaming at different times |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111645269.1A CN114354793A (en) | 2021-12-30 | 2021-12-30 | Method for constructing high performance liquid fingerprint sugar spectrum of polygonatum sibiricum decoction pieces prepared by steaming at different times |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114354793A true CN114354793A (en) | 2022-04-15 |
Family
ID=81104338
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111645269.1A Pending CN114354793A (en) | 2021-12-30 | 2021-12-30 | Method for constructing high performance liquid fingerprint sugar spectrum of polygonatum sibiricum decoction pieces prepared by steaming at different times |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114354793A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115327002A (en) * | 2022-07-14 | 2022-11-11 | 安徽中医药大学 | Quality evaluation method for distinguishing polygonatum cyrtonema from prepared polygonatum cyrtonema and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109425680A (en) * | 2017-08-30 | 2019-03-05 | 中央民族大学 | A method of Ferula sinkiangensis polysaccharide fingerprinting is established using HPLC-MS |
-
2021
- 2021-12-30 CN CN202111645269.1A patent/CN114354793A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109425680A (en) * | 2017-08-30 | 2019-03-05 | 中央民族大学 | A method of Ferula sinkiangensis polysaccharide fingerprinting is established using HPLC-MS |
Non-Patent Citations (2)
| Title |
|---|
| 吴丰鹏等: "九蒸九制对黄精多糖单糖组成及其抗氧化性的影响", 《食品工业科技》, vol. 42, no. 2, pages 42 - 46 * |
| 赵欣等: "黄精的高效液相色谱指纹图谱", 《西北农业学报》, vol. 20, no. 2, pages 114 - 119 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115327002A (en) * | 2022-07-14 | 2022-11-11 | 安徽中医药大学 | Quality evaluation method for distinguishing polygonatum cyrtonema from prepared polygonatum cyrtonema and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ai et al. | Rapid determination of the monosaccharide composition and contents in tea polysaccharides from Yingshuang green tea by pre-column derivatization HPLC | |
| Zhao et al. | Advanced analysis of polysaccharides, novel functional components in food and medicine dual purposes Chinese herbs | |
| CN112724184B (en) | Method for separating and preparing special compound arginine diglycoside AFG in ginseng | |
| CN109283269A (en) | The analysis method of feature alkaloid composition in a kind of fresh Huoshan rice dry measure used in former times | |
| CN111035666A (en) | Ginseng extract with high content of rare saponin, ginseng wine and ginseng oral liquid | |
| Rovkina et al. | Water-soluble polysaccharides of Alfalfa (Medicago sativa (Fabaceae)) of flora of Krasnoyarsk krai | |
| CN110128562A (en) | A kind of antitumor Psoralen lipopolysaccharides and its extraction separation method and the application in terms of preparing anti-tumor drug | |
| CN114354793A (en) | Method for constructing high performance liquid fingerprint sugar spectrum of polygonatum sibiricum decoction pieces prepared by steaming at different times | |
| CN108892739A (en) | A kind of method for extraction and purification of anemaran | |
| CN107056851A (en) | A kind of preparation method of the total oligosaccharide of Morinda officinalis | |
| CN108997510B (en) | Anti-oxidation application of galangal polysaccharides and separation and purification method thereof | |
| CN111272895A (en) | Method for simultaneously and rapidly detecting amino sugar, neutral sugar and uronic acid in seaweed | |
| CN112679564B (en) | Method for separating and purifying specific compound arginine glycoside AF of ginseng | |
| CN110412196B (en) | Quality control method for rhizoma polygonati medicinal material | |
| CN106690244B (en) | Preparation and application of novel sweet gynostemma pentaphylla sweetener | |
| CN108424469B (en) | Gorgon fruit kernel polysaccharide and separation and extraction method and application thereof | |
| CN113603802B (en) | Preparation method and application of verbena polysaccharide | |
| CN112961261B (en) | Yangchun sand rhizome polysaccharide, preparation method thereof and anti-oxidation application thereof | |
| CN109234335B (en) | Preparation method of polysaccharide rich in galactofuranose in tabasheer | |
| Liu et al. | Quality markers of Dendrobium officinale by “oligosaccharide-spectrum-effect” relationships | |
| CN110412197B (en) | Method for constructing HPLC fingerprint spectrum of rhizoma polygonati medicinal material | |
| CN110483657B (en) | Chinese lobelia homogeneous polysaccharide and preparation method and application thereof | |
| CN112691163A (en) | Rhizoma bletillae lung-heat clearing and stomach nourishing oral liquid and preparation method thereof | |
| CN101717452B (en) | Method for preparing neutral polysaccharide from gynostemma pentaphylla | |
| CN115109168B (en) | Gentiana rigescens neutral polysaccharide and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |