CN114354708A - Regeneration electrode and preparation method thereof - Google Patents
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- CN114354708A CN114354708A CN202111562089.7A CN202111562089A CN114354708A CN 114354708 A CN114354708 A CN 114354708A CN 202111562089 A CN202111562089 A CN 202111562089A CN 114354708 A CN114354708 A CN 114354708A
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Abstract
The invention belongs to the technical field of biosensors, and particularly discloses a regeneration electrode and a preparation method thereof. The preparation method of the regeneration electrode comprises the following steps: cleaning a working electrode of the failure sensor by using a cleaning agent to obtain an electrode 1; the cleaning agent contains inorganic alkali and alcohol; the failure sensor comprises a working electrode, wherein a mixture of at least one of chitosan, carbon nano tubes, carbon aerogel and Prussian blue and a binder is covered on the working electrode; and sequentially carrying out cyclic voltammetry scanning on the electrode 1 in KCl/PBS buffer solution and inorganic alkali solution to obtain a regeneration electrode. The method can effectively remove materials such as Nafion films, chitosan, carbon nanotubes, carbon aerogel, Prussian blue and the like attached to the failure sensor, does not need to use dangerous reagents with strong corrosivity such as hydrochloric acid, sulfuric acid and the like, and can recycle the electrodes for preparing the sensor again.
Description
Technical Field
The invention relates to the technical field of biosensors, in particular to a regeneration electrode and a preparation method thereof.
Background
The wearable flexible glucose sensor can realize non-invasive detection of blood sugar, so that a blood sugar detector is prevented from being painful due to needle insertion. The gold has the advantages of strong stability, high conductivity, good biocompatibility and the like, and can be combined with polyethylene terephthalate (PET) and silver/silver chloride to prepare a flexible gold three-electrode taking a gold electrode as a working electrode and a reference electrode and taking the silver/silver chloride as the reference electrode. The flexible gold three-electrode glucose sensor for non-invasive detection of blood glucose can be prepared by further modifying the flexible gold three-electrode with materials such as prussian blue, chitosan, glucose oxidase, carbon nano tube and carbon aerogel. For example, the related technology discloses a preparation method and application of a glucose sensor with a PET substrate thin film gold electrode, wherein the PET substrate thin film gold electrode is obtained by chemical gold plating, silver paste is coated on a reference electrode, Prussian blue is deposited on a working electrode, glucose oxidase/chitosan/carbon nanotube solution is dripped for modification, and then perfluorinated sulfonic acid group polymer Nafion solution is dripped for packaging, so that the flexible glucose sensor is prepared.
After the flexible gold three-electrode glucose sensor is prepared, the flexible gold three-electrode glucose sensor cannot be continuously used and fails due to pollution of a detection sample, inactivation of glucose oxidase and the like. The flexible gold three-electrode has higher preparation cost, and the undamaged flexible gold three-electrode can be used for preparing the flexible gold three-electrode glucose sensor again after being processed, so that the flexible gold three-electrode has higher recycling value.
The treatment and recycling of the flexible gold three-electrode needs to solve the following problems: 1. the surface layer of the flexible gold three-electrode glucose sensor is usually embedded and encapsulated by Nafion solution, and the Nafion embedded film on the surface of the electrode needs to be removed firstly. However, no relevant art discloses how to remove the Nafion embedded membrane. 2. Materials such as chitosan, carbon nano tubes, carbon aerogel, prussian blue and the like have strong adhesion, and need to be removed cleanly under the condition of not damaging the gold electrode, while the existing method inevitably damages the gold electrode when removing the materials such as chitosan, carbon nano tubes, carbon aerogel, prussian blue and the like. 3. The use of ultrasonic cleaning results in silver/silver chloride exfoliation as a reference electrode. 4. The electrode is easily corroded by cleaning the electrode with hydrochloric acid, sulfuric acid and the like, and a polarization phenomenon is easily generated when cyclic voltammetry is used for detection. Strong hydrochloric acid and sulfuric acid solutions have certain operational safety risks in the process of using and preparing reagents.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. Therefore, a first object of the present invention is to provide a method for manufacturing a regenerated electrode, which can effectively remove materials such as Nafion membrane, chitosan, carbon nanotube, carbon aerogel, prussian blue, etc. attached to a failed sensor, for example, a flexible gold three-electrode glucose sensor, and can reuse the electrode without using hazardous reagents with strong corrosivity such as hydrochloric acid, sulfuric acid, etc. to manufacture the sensor again.
It is a second object of the present invention to provide a regeneration electrode.
Specifically, the invention relates to the following technical scheme:
a preparation method of a regeneration electrode comprises the following steps:
cleaning a working electrode of the failure sensor by using a cleaning agent to obtain an electrode 1; the cleaning agent contains inorganic alkali and alcohol; the failure sensor comprises a working electrode, wherein a mixture of at least one of chitosan, carbon nano tubes, carbon aerogel and Prussian blue and a binder is covered on the working electrode;
and sequentially carrying out cyclic voltammetry scanning on the electrode 1 in KCl/PBS buffer solution and inorganic alkali solution to obtain a regeneration electrode.
In the preparation method, the working electrode of the failure sensor is cleaned by adopting a cleaning agent consisting of inorganic base and alcohol, wherein the alcohol can dissolve a binder for fixing modification materials such as chitosan, carbon nanotubes, carbon aerogel, Prussian blue and the like on the working electrode, so that the binder is removed.
The chitosan in the sensor is generally dissolved in an acidic solution such as acetic acid, and then uniformly mixed and dispersed with carbon nanotubes, carbon aerogel and the like, and then glucose oxidase and the like are adsorbed for preparing the corresponding sensor. The chitosan has viscosity and can be adhered to the surface of the electrode material after being dried in the air. Namely, chitosan, carbon nanotubes and carbon aerogel in the failure sensor present certain acidity. After inorganic base is added into the cleaning agent, the interior of the chitosan and the connection between the chitosan and materials such as carbon nano tubes, carbon aerogel and the like can be damaged through acid-base neutralization, so that the chitosan is dispersed and further removed from the working electrode.
Prussian blue, ferric ferrocyanide, is a substance that can be synthesized by coordination of potassium ferrocyanide and ferric chloride in a slightly acidic environment. During the preparation process of the sensor, the Prussian blue can be adhered to the surface of the electrode through dyeing and electrochemical deposition. The dissolving effect of the ethanol on the Prussian blue is poor, but the structure of the Prussian blue can be destroyed by adding the inorganic base and neutralizing the solution with acid and alkali to form ferric hydroxide and ferrocyanide, so that the solution has a good dissolving and cleaning effect on the Prussian blue.
Therefore, the working electrode of the failure sensor is cleaned by adopting the cleaning agent consisting of the inorganic base and the alcohol, and materials such as the adhesive, the chitosan, the carbon nano tube, the carbon aerogel, the Prussian blue and the like which are adhered on the working electrode can be well dissolved and fall off.
Meanwhile, by performing cyclic voltammetry scanning in a KCl/PBS buffer solution and an inorganic alkali solution, deposits and oxides on the working electrode can be removed, and the polarization phenomenon of the working electrode is improved.
In some embodiments of the invention, the cleaning agent comprises 1-4 parts by volume of inorganic alkali solution and 1-2 parts by volume of alcohol; preferably, the cleaning agent comprises 1-4 parts of inorganic alkali solution and 1 part of alcohol by volume; more preferably, the cleaning agent comprises 1-2 parts of inorganic alkali solution and 1 part of alcohol by volume.
In some embodiments of the present invention, the inorganic alkali solution in the cleaning agent is an aqueous solution of an inorganic alkali, and the concentration is 1 to 3mol/L, preferably 1.5 to 2.5 mol/L. After the inorganic alkali solution with the concentration is mixed with the alcohol according to a proportion, the pH value of the cleaning agent can reach 10 or above.
In some embodiments of the present invention, the inorganic base in the cleaning agent comprises at least one of sodium hydroxide, potassium hydroxide, magnesium hydroxide, sodium carbonate, and potassium carbonate. To avoid unnecessary ions, the inorganic base in the cleaning agent is preferably sodium hydroxide.
In some embodiments of the invention, the alcohol in the cleaning agent comprises at least one of ethanol, methanol, and isopropanol. Compared with other alcohols such as isopropanol and methanol, ethanol has the advantages of low price, strong volatility, difficult residue, low toxicity and irritation and the like, so that ethanol is preferred.
In some embodiments of the present invention, the step of cleaning the working electrode of the failed sensor with the cleaning agent is specifically to apply the cleaning agent to the working electrode of the failed sensor and stir the mixture covered on the working electrode. The agitation may be performed by using a wash bar. The cleaning agent is combined with mechanical stirring, so that modification materials such as a binder, chitosan, a carbon nano tube, carbon aerogel, Prussian blue and the like adhered to the working electrode can be well dissolved and fall off.
In some embodiments of the present invention, the step of cleaning the working electrode of the failed sensor with a cleaning agent comprises the steps of washing with water and drying. And the cleaning agent is removed from the surface of the working electrode through water washing and drying, and the influence on the subsequent cyclic voltammetry scanning step is avoided.
In some embodiments of the invention, the binder comprises a Nafion membrane, a perfluorosulfonic polymer. The Nafion solution is prepared by dissolving perfluorosulfonic acid polymer with volatile alcohol such as isopropanol and ethanol, and then air-dried to form a selectively permeable polymer membrane (Nafion membrane), which can encapsulate and embed the electrode modification material and adhere to the surface of the electrode. The addition of ethanol to the cleaning agent can dissolve the polymer film formed by the Nafion solution.
In some embodiments of the invention, the working electrode is a gold electrode.
In some embodiments of the invention, the voltage range for cyclic voltammetry scans in KCl/PBS buffer is 300-650 mV; preferably, the scan rate is 100-200 mV/s, preferably about 150 mV/s; preferably, the number of scanning turns is 3-9 turns. Deposits and oxides on the working electrode were removed by cyclic voltammetry scans in KCl/PBS buffer.
In some embodiments of the present invention, the concentration of KCl in the KCl/PBS buffer solution is 0.05-0.2 mol/L, preferably 0.1-0.15 mol/L. The KCl concentration has influence on the current of the electrode, and when cyclic voltammetry scanning is carried out at an excessively high KCl concentration, the generated current is excessively high, so that the electrode is possibly burnt out; if it is too low, the current is insufficient and good effects of removing the adherent and activating the electrode cannot be obtained.
In some embodiments of the invention, the cyclic voltammetry scans in KCl/PBS buffer are performed by applying KCl/PBS buffer to the working electrode and then performing cyclic voltammetry scans.
In some embodiments of the invention, the voltage range for the cyclic voltammetry scan in an inorganic base solution is-250 mV to 300 mV; preferably, the scan rate is 30-80 mV/s, preferably about 50 mV/s; preferably, the number of scanning turns is 1-3 turns. By performing secondary cyclic voltammetry scanning in an inorganic alkali solution, deposits and oxides on the working electrode can be further removed, and the polarization phenomenon of the working electrode is improved.
In some embodiments of the present invention, the inorganic base solution used for cyclic voltammetry scanning is an aqueous solution of an inorganic base, and the concentration thereof is 0.05 to 1mol/L, preferably 0.1 to 0.5 mol/L. The type of the inorganic base used for cyclic voltammetry scanning comprises at least one of sodium hydroxide, potassium hydroxide, magnesium hydroxide, sodium carbonate, potassium carbonate, sodium bicarbonate and potassium bicarbonate, and the type of the inorganic base can be the same as or different from that of the inorganic base in the cleaning agent. Preferably, the inorganic base used for cyclic voltammetry scanning is sodium hydroxide. Preferably, the inorganic base used for cyclic voltammetry scans is the same as the inorganic base in the cleaning agent.
In some embodiments of the invention, the cyclic voltammetry scans in the inorganic base solution are performed by applying the inorganic base solution to the working electrode and then performing the cyclic voltammetry scans.
In some embodiments of the invention, the step of performing cyclic voltammetry scans in PBS buffer is further included after performing cyclic voltammetry scans in an inorganic base solution. By performing cyclic voltammetry scanning in PBS buffer, a stable and repeated scanning curve can be obtained, and the stability of the regeneration electrode is checked.
In some embodiments of the invention, the voltage range for cyclic voltammetry scans in PBS buffer is-250 mV to 300 mV; preferably, the scan rate is 30-80 mV/s, preferably about 50 mV/s; preferably, the number of scanning turns can be determined as required until a stable and repeated scanning curve is obtained. As an example, the number of scan turns may be 3-6 turns.
In some embodiments of the present invention, the pH of the PBS buffer used for cyclic voltammetry scanning is 6 to 8, preferably 7 to 7.5, and more preferably 7 to 7.2.
In some embodiments of the invention, the cyclic voltammetric scan is performed in PBS buffer by applying PBS buffer to the working electrode and then performing the cyclic voltammetric scan.
The failure sensor further comprises a counter electrode and a reference electrode, the counter electrode, the reference electrode and the working electrode form a three-electrode area, and when cleaning is carried out by adopting a cleaning agent and cyclic voltammetry scanning is carried out in a KCl/PBS buffer solution, an inorganic alkali solution and a PBS buffer solution, the three-electrode area can be covered by the cleaning agent, the KCl/PBS buffer solution, the inorganic alkali solution and the PBS buffer solution in the adding amount. By way of example, the addition amount of the cleaning agent, the KCl/PBS buffer solution for cyclic voltammetry scanning, the inorganic alkali solution or the PBS buffer solution is not less than 7 mu L/mm of the area ratio of the working electrode2Preferably 7 to 15. mu.L/mm2More preferably 7.5 to 10. mu.L/mm2. For example, 30 to 40. mu.L of a cleaning agent, KCl/PBS buffer solution, inorganic alkali solution or PBS buffer solution may be added to a circular working electrode having a diameter of 4 mm.
And (3) after scanning by cyclic voltammetry in PBS buffer solution, washing and drying the regeneration electrode, and reusing the regeneration electrode in the preparation of various sensors or other electronic elements.
A regeneration electrode is prepared by the preparation method.
Compared with the prior art, the invention has the following beneficial effects:
the detergent is prepared from alcohol and inorganic base, is simple in preparation, low in cost and small in dosage, has a good dissolving and removing effect on materials such as nafion films, chitosan, carbon nanotubes, carbon aerogel, prussian blue and the like attached to the failure sensor, and does not need to use dangerous reagents with strong corrosivity such as hydrochloric acid and sulfuric acid.
And the KCl/PBS buffer solution is adopted for cyclic voltammetry scanning to remove deposits and oxides on the gold electrode, and the method is simple and convenient to operate, safe and efficient.
And (3) scanning by adopting an inorganic alkali solution through cyclic voltammetry to further remove residual deposits and oxides on the gold electrode and obviously improve the polarization phenomenon of the electrode.
The regenerated anode obtained by treating the failed sensor according to the method disclosed by the invention has very good electrical stability and can be applied to manufacturing various sensors or other electronic components.
Drawings
FIG. 1 is a photograph of a real object during cleaning of a failed gold three-electrode glucose sensor of example 1;
FIG. 2 is a plot of cyclic voltammetry scans (CV curves) of the failed gold three-electrode glucose sensors of example 1 and comparative example 1 after regeneration treatment;
FIG. 3 is a photograph of a sample from a cleaning process of a failed gold three-electrode glucose sensor of example 2 and comparative example 2;
fig. 4 shows flexible gold three-electrode cleaners formulated in different proportions for example 2 and comparative example 2.
Detailed Description
The technical solution of the present invention is further described below with reference to specific examples. The starting materials used in the following examples, unless otherwise specified, are available from conventional commercial sources; the processes used, unless otherwise specified, are conventional in the art.
Example 1
A flexible gold three-electrode glucose sensor having a working electrode with a diameter of 4mm and an overall width of 10mm is exemplified below, and as shown in fig. 1a1, the flexible gold three-electrode glucose sensor comprises a flexible PET substrate coated with gold three-electrodes, including a gold electrode as a working electrode (circular area in fig. 1) and a counter electrode, and silver/silver chloride as a reference electrode, wherein the working electrode is covered with chitosan, carbon nanotubes, carbon aerogel, a mixture of prussian blue and Nafion.
The regeneration treatment method of the ineffective flexible gold three-electrode glucose sensor comprises the following steps:
(1) preparing 2mol/L sodium hydroxide solution, and then mixing 1 part of 2mol/L sodium hydroxide solution and 1 part of absolute ethyl alcohol to prepare the flexible gold three-electrode cleaning agent. 40 μ L of flexible gold three-electrode cleaner was dropped on the gold three-electrode, and agitated with a plastic rod having a diameter of 1mm, and the Nafion film for encapsulating the electrode was dissolved and removed, and chitosan, carbon nanotubes, carbon aerogel, prussian blue, etc. adhered to the surface of the working electrode, as shown in fig. 1a 2. Then, the gold three electrodes were rinsed with ultra-pure water and dried with cold air to obtain cleaned three electrodes as shown in fig. 1a 3.
(2) 40 μ L of 0.1M KCl/PBS buffer was added dropwise to the gold three electrode, and cyclic voltammetry was performed at a rate of 150mV/s for 6 cycles at 300mV to 650mV, to remove deposits and oxides from the gold three electrode.
(3) 40 mu L of 0.5mol/L sodium hydroxide solution is dripped on the gold three-electrode, and cyclic voltammetry scanning is carried out for 1 circle at the speed of 50mV/s from minus 250mV to 300mV, so as to further remove residual sediment and oxide on the gold three-electrode and improve the polarization phenomenon of the electrode.
(4) 40 μ L of pH 7.2 PBS buffer was added dropwise to the gold three electrode and cyclic voltammetry was performed at-250 mV to 300mV at a rate of 50mV/s for 3 cycles to obtain a stable, repeating CV curve, as shown in FIG. 2.
(5) The gold three electrodes are washed by ultrapure water and dried by cold air.
Example 2
In this embodiment, a failed flexible gold three-electrode glucose sensor is subjected to a regeneration treatment, where the structure of the failed flexible gold three-electrode glucose sensor is the same as that in embodiment 1, and a real photograph is shown in fig. 3a1, where the regeneration treatment method includes the following steps:
(1) preparing 2mol/L sodium hydroxide solution, and then mixing 2 parts of the 2mol/L sodium hydroxide solution and 1 part of absolute ethyl alcohol to prepare the clear and transparent flexible gold three-electrode cleaning agent, as shown in figure 4A. Dropping 30 μ L of flexible gold three-electrode cleaner on the gold three-electrode, stirring with a plastic rod with a diameter of 1mm, dissolving and removing the Nafion film for encapsulating the electrode, and adhering chitosan, carbon nanotubes, carbon aerogel, Prussian blue and other materials on the surface of the working electrode, as shown in FIG. 3A 2. Then, the gold three electrodes are washed by using ultrapure water and dried by cold air blow, and the cleaned gold three electrodes are obtained, as shown in fig. 3a 3.
(2) 30 μ L of 0.1M KCl/PBS buffer was added dropwise to the gold three-electrode, and cyclic voltammetry was performed at a rate of 150mV/s for 3 cycles at 300mV to 650mV, to remove deposits and oxides from the gold three-electrode.
(3) 30 mu L of 0.1mol/L sodium hydroxide is dripped on the gold three-electrode, and cyclic voltammetry is carried out for 2 circles at the speed of 50mV/s from minus 250mV to 300mV, so as to further remove residual deposits and oxides on the gold three-electrode and improve the polarization phenomenon of the electrode.
(4) mu.L of pH 7.2 PBS buffer was added dropwise to the gold three electrode and cyclic voltammetry was carried out at-250 mV to 300mV at a rate of 50mV/s for 3 cycles to obtain a stably repeated CV curve similar to that of example 1.
(5) The gold electrode is washed by ultrapure water and dried by cold air.
Comparative example 1
This comparative example differs from example 1 in that: cyclic voltammetric scans were not performed in 0.5mol/L sodium hydroxide solution. The method specifically comprises the following steps:
(1) preparing 2mol/L sodium hydroxide solution, and then mixing 1 part of 2mol/L sodium hydroxide solution and 1 part of absolute ethyl alcohol to prepare the flexible gold three-electrode cleaning agent. Dropping 40 μ L of flexible gold three-electrode cleaner on the gold three-electrode, stirring with a plastic rod with a diameter of 1mm, dissolving and removing the Nafion film for packaging the electrode, and adhering chitosan, carbon nanotube, carbon aerogel, Prussian blue and other materials on the surface of the gold electrode. And then washing the gold three-electrode by using ultrapure water, and drying by cold air to obtain the cleaned gold three-electrode.
(2) mu.L of 0.1M KCl/PBS buffer was added dropwise to the electrode, and cyclic voltammetry was performed at a rate of 150mV/s for 6 cycles at 300mV to 650mV, to remove deposits and oxides on the gold electrode.
(3) 40 μ L of pH 7.2 PBS buffer was added dropwise to the gold three electrode, and cyclic voltammetry was performed at-250 mV to 300mV at a rate of 50mV/s for 3 cycles, and the resulting CV curve was as shown in FIG. 2. As can be seen from comparison with the CV curve of example 1, it was difficult to obtain a stably repeated CV curve because the polarization phenomenon of the electrode could not be improved after omitting the cyclic voltammetry scanning step in a 0.5mol/L sodium hydroxide solution.
Comparative example 2
This comparative example performs a regeneration treatment of a failed flexible gold three-electrode glucose sensor, wherein the structure of the failed gold three-electrode glucose sensor is the same as that of example 2, and a physical photograph is shown in fig. 3B1, and the regeneration treatment method differs from that of example 2 in that: the composition of the flexible gold three-electrode cleaning agent is changed into that: 1 part of 2mol/L sodium hydroxide solution and 9 parts of absolute ethyl alcohol.
The method comprises the following specific steps:
preparing 2mol/L sodium hydroxide solution, and then mixing 1 part of 2mol/L sodium hydroxide solution and 9 parts of absolute ethyl alcohol to obtain the flexible gold three-electrode cleaning agent, wherein the flexible gold three-electrode cleaning agent is obviously turbid, as shown in figure 4B. Dropping 30 μ L of flexible gold three-electrode cleaner on the gold three-electrode, stirring with plastic rod with diameter of 1mm, and dissolving and removing Nafion film for packaging electrode, chitosan, carbon nanotube, carbon aerogel, Prussian blue, etc. adhered on the surface of working electrode with the electrode material graph shown in FIG. 3B 2. The gold three electrodes were then rinsed with ultra-pure water and blown dry with cold air, as shown in fig. 3B 3.
Fig. 4B and fig. 3B2 and B3 reflect that when the formulation ratio of the flexible gold three-electrode cleaner is incorrect, not only the solution becomes turbid, but also the cleaning effect on the failed gold three-electrode is not good, and the materials such as the Nafion film of the encapsulated electrode, chitosan adhered to the surface of the working electrode, carbon nanotubes, carbon aerogel, prussian blue and the like cannot be effectively dissolved and removed.
The cleaned sensor (fig. 3C1) was cleaned again using the flexible gold three-electrode cleaner prepared from 1 part of 2mol/L sodium hydroxide solution and 9 parts of absolute ethanol according to the method of example 2, and the specific steps were as follows:
2 parts of 2mol/L sodium hydroxide solution and 1 part of absolute ethyl alcohol are mixed to prepare the clear and transparent flexible gold three-electrode cleaning agent. Dropping 30 μ L of flexible gold three-electrode cleaner on the gold three-electrode, stirring with a plastic rod with a diameter of 1mm, dissolving and removing the Nafion film for encapsulating the electrode, and adhering chitosan, carbon nanotubes, carbon aerogel, Prussian blue and other materials on the surface of the working electrode, as shown in FIG. 3C 2. Then, the gold three electrodes are washed by using ultrapure water, and dried by cold air blow, so that the cleaned three electrodes are obtained, as shown in fig. 3C 3. It can be seen that the modification material attached to the electrode can be well dissolved and cleaned by using a suitable cleaning agent.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. A method for preparing a regeneration electrode is characterized in that: the method comprises the following steps:
cleaning a working electrode of the failure sensor by using a cleaning agent to obtain an electrode 1; the cleaning agent contains inorganic alkali and alcohol; the failure sensor comprises a working electrode, wherein a mixture of at least one of chitosan, carbon nano tubes, carbon aerogel and Prussian blue and a binder is covered on the working electrode;
and sequentially carrying out cyclic voltammetry scanning on the electrode 1 in KCl/PBS buffer solution and inorganic alkali solution to obtain a regeneration electrode.
2. The method for producing a regeneration electrode according to claim 1, characterized in that: the cleaning agent comprises 1-4 parts of inorganic alkali solution and 1-2 parts of alcohol by volume.
3. The method for producing a regeneration electrode according to claim 2, characterized in that: the concentration of the inorganic alkali solution in the cleaning agent is 1-3 mol/L.
4. The method for producing a regeneration electrode according to claim 1, characterized in that: the inorganic base in the cleaning agent comprises at least one of sodium hydroxide, potassium hydroxide, magnesium hydroxide, sodium carbonate and potassium carbonate.
5. The method for producing a regeneration electrode according to claim 1, characterized in that: the alcohol in the cleaning agent comprises at least one of ethanol, methanol and isopropanol.
6. The method for producing a regeneration electrode according to any one of claims 1 to 5, characterized in that: the voltage range of cyclic voltammetry scanning in KCl/PBS buffer solution is 300-650 mV.
7. The method for producing a regeneration electrode according to claim 6, characterized in that: the concentration of the KCl/PBS buffer solution is 0.05-0.2 mol/L.
8. The method for producing a regeneration electrode according to claim 6, characterized in that: the voltage range scanned by cyclic voltammetry in inorganic alkali solution is-250 mV-300 mV.
9. The method for producing a regeneration electrode according to claim 8, characterized in that: the concentration of the inorganic alkali solution used for cyclic voltammetry scanning is 0.05-1 mol/L.
10. A regenerated electrode obtained by the production method according to any one of claims 1 to 9.
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