CN114354592A - Detection device and detection method for rapidly detecting residual pesticide - Google Patents
Detection device and detection method for rapidly detecting residual pesticide Download PDFInfo
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- CN114354592A CN114354592A CN202210023426.3A CN202210023426A CN114354592A CN 114354592 A CN114354592 A CN 114354592A CN 202210023426 A CN202210023426 A CN 202210023426A CN 114354592 A CN114354592 A CN 114354592A
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- 238000001514 detection method Methods 0.000 title claims abstract description 117
- 239000000575 pesticide Substances 0.000 title claims abstract description 23
- 238000003756 stirring Methods 0.000 claims abstract description 28
- 239000000243 solution Substances 0.000 claims description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 239000000758 substrate Substances 0.000 claims description 25
- 102000004190 Enzymes Human genes 0.000 claims description 20
- 108090000790 Enzymes Proteins 0.000 claims description 20
- 239000007853 buffer solution Substances 0.000 claims description 20
- 239000003795 chemical substances by application Substances 0.000 claims description 20
- 229940088598 enzyme Drugs 0.000 claims description 20
- 238000002360 preparation method Methods 0.000 claims description 20
- 239000012153 distilled water Substances 0.000 claims description 15
- 239000008213 purified water Substances 0.000 claims description 15
- 239000000447 pesticide residue Substances 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- 102000003914 Cholinesterases Human genes 0.000 claims description 5
- 108090000322 Cholinesterases Proteins 0.000 claims description 5
- 238000002835 absorbance Methods 0.000 claims description 5
- 230000005540 biological transmission Effects 0.000 claims description 5
- 239000000872 buffer Substances 0.000 claims description 5
- 229940048961 cholinesterase Drugs 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 5
- 239000012535 impurity Substances 0.000 claims description 5
- 230000005764 inhibitory process Effects 0.000 claims description 5
- 230000003750 conditioning effect Effects 0.000 claims description 4
- 239000012634 fragment Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 235000021384 green leafy vegetables Nutrition 0.000 claims description 3
- 235000012055 fruits and vegetables Nutrition 0.000 claims description 2
- 239000008363 phosphate buffer Substances 0.000 claims 1
- 238000012031 short term test Methods 0.000 claims 1
- 239000002699 waste material Substances 0.000 abstract 1
- 239000008055 phosphate buffer solution Substances 0.000 description 4
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 244000141359 Malus pumila Species 0.000 description 2
- 240000008790 Musa x paradisiaca Species 0.000 description 2
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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Abstract
The invention provides a detection device and a detection method for rapidly detecting residual pesticide, which comprises the following steps: the casing, top one side of casing is equipped with the support frame, a plurality of reaction boxes can detect simultaneously, a large amount of time has been saved, and stir solution through the stirring motor, can make solution stirring more even, save manual stirring, work efficiency is improved, detect the solution configuration and accomplish the back, do not need the manual work to fall into the cell with detecting solution, then in putting into the sample detection groove of another one, the operation of closing the apron, not only waste a large amount of time like this, it can't in time put into the sample detection groove to have guaranteed still to detect solution, lead to the detected data to have certain inaccurate bear, and the time that the sample detection groove was put into to every detection solution is different, carry out the machine-in detection time measuring to detection solution, the check-out time of every detection solution is different, lead to the data that detect inaccurate like this, influence the experimental result.
Description
Technical Field
The invention relates to the technical field of agricultural product detection equipment, in particular to a detection device and a detection method for rapidly detecting residual pesticides.
Background
As is known, China is a big agricultural country, is also a big pesticide production country and a big pesticide using country, and pesticides play a vital role in preventing and treating plant diseases and insect pests, removing weeds and regulating the growth of crops, so that the stable yield and the high yield of the crops are promoted. In particular, organophosphorus and carbamate pesticides are widely used in the production process of agricultural products such as vegetables, fruits and the like, and the organophosphorus and carbamate pesticides are used for a long time in agriculture and have strong toxicity to human and animals. With the improvement of living standard of people, pesticide residue in the environment and residual toxicity of agricultural products with pesticide residue are generally concerned.
The existing rapid pesticide residue detection device has certain defects, more required instruments, complicated steps and inconvenience in use.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a detection device and a detection method for rapidly detecting residual pesticides.
In order to achieve the purpose of the invention, the invention adopts the technical scheme that:
a detection device for rapidly detecting residual pesticide comprises: a shell, wherein a supporting frame is arranged on one side above the shell, a reaction box is arranged on the supporting frame, a stirring motor is arranged at the bottom of the reaction box, a stirring rod is arranged inside the reaction box and connected with a rotating shaft of the stirring motor, a filter screen is arranged inside the reaction box and positioned above the stirring rod, a solenoid valve is arranged on one side of the bottom of the reaction box, a communicating pipe is arranged below the solenoid valve, a control panel is arranged on one side of the top of the shell away from the supporting frame, a display screen is arranged on the control panel, a detection placing groove is arranged above the shell and positioned between the supporting frame and the control panel, a sample detection groove is arranged at the bottom of the detection placing groove, a cover plate is arranged on one side of the detection placing groove, a drip nozzle is arranged on the cover plate, and one end of the drip nozzle is connected with one end of the communicating pipe away from the reaction box, the improved portable electronic scale is characterized in that an electronic scale is arranged above the shell, a laser is arranged inside the shell, a photoelectric receiving tube is arranged inside the shell, a power supply transmission module is arranged on one side inside the shell, a main control module is arranged on one side, away from the power supply transmission module, of the inside of the shell, a signal conditioning module is arranged on one side, close to the main control module, of the inside of the shell, and a supporting seat is arranged below the shell.
As an improvement, a cuvette is arranged inside the sample detection groove.
As an improvement, the laser and the photoelectric receiving tube are correspondingly arranged on two sides of the sample detection groove.
As an improvement, after the cover plate is closed, the drip nozzle is positioned right above the sample detection groove.
As an improvement, the display screen is a touch screen.
As a modification, the drip nozzle and the cover plate can be disassembled.
As an improvement, the communicating pipe is a hose.
As an improvement, the reaction box is made of glass materials, and the reaction box and the support frame can be disassembled.
The detection method applying the detection device for rapidly detecting the residual pesticide is characterized by comprising the following steps of:
step one, plugging a power supply, turning on a detection device for rapidly detecting residual pesticides, displaying a picture on a display screen, prompting operation according to the picture, starting self-detection, and about 1 min. And after the self-checking is finished, entering a standby detection state.
Step two, preparing a detection reagent:
1. buffer solution: adding 1 bag of buffer into 500mL of distilled water or purified water, stirring and dissolving to obtain phosphate buffer solution pH7.6, and storing at room temperature.
2. Color developing agent: dissolving 1 bottle of color developing agent in 25mL of buffer solution, and storing 100 μ L in a refrigerator at 4 deg.C when using.
3. Substrate: dissolving 1 bottle of substrate with 12.5mL of distilled water or purified water, and storing in a refrigerator at 4 deg.C with 100 μ L when in use; or dissolving 1 bottle of substrate in 2.5mL of distilled water or purified water, and storing at 4 deg.C in 20 μ L refrigerator.
4. Cholinesterase: the enzyme preparation can be directly used without preparation, and 100 μ L of the enzyme preparation can be stored in a refrigerator at 4 deg.C.
Note: the reagent manufacturers are different, and the preparation is subject to the instruction.
And step three, taking 2g of fruit and vegetable samples (4 g of tubers), cutting the leaf vegetables into fragments with the square size of about 25px, taking the cross section samples or the skins of the tubers, putting the tubers into a triangular flask, adding 10mL of buffer solution, oscillating for 1-2 min, pouring out the extracting solution, standing for 2min, and waiting for detection. If the extract is turbid or contains too much impurities, it can be filtered and then measured.
And step three, firstly, carrying out comparison test, adding 2.5mL of buffer solution into a reaction bottle, then respectively adding 100 mu L of enzyme solution and color developing agent, uniformly mixing, standing for reaction for 10min, adding 100 mu L or 20 mu L of substrate, shaking uniformly, immediately pouring into a cuvette, timely putting into a sample detection tank, closing a cover plate, adjusting a test interface of parameters to be detected through a display screen on a control panel, starting to carry out comparison through a comparison button on a touch display screen, finishing comparison after 3 min, taking out a comparison sample, and keeping the interface of a display unchanged. And then carrying out sample detection, namely firstly putting the cuvette into a sample detection groove, closing a cover plate, putting 2.5ml of extracting solution into a reaction box, then respectively adding 100 mu L of enzyme solution and color developing agent, uniformly mixing by a motor, standing for 10min, then adding 100 mu L or 20 mu L of substrate, uniformly mixing, immediately opening an electromagnetic valve, enabling the solution to enter the cuvette through a communicating pipe, passing through a dripping nozzle, detecting by a touch display screen, and displaying the absorbance increment delta alpha t) and the inhibition rate by the display screen after the detection is finished. And the data is automatically saved.
The invention has the beneficial effects that:
the detection device for rapidly detecting the residual pesticide is provided with the electronic scale, so that the sample can be conveniently measured, the loss of the electronic scale can be effectively prevented, the detection device for rapidly detecting the residual pesticide is provided with the reaction boxes, the stirring motors are arranged at the bottoms of the reaction boxes, the reaction boxes can simultaneously detect, a large amount of time is saved, the solution is stirred by the stirring motors, the solution can be stirred more uniformly, manual stirring is omitted, the working efficiency is improved, after the detection solution is prepared, the detection solution does not need to be poured into the cuvette manually and then put into the sample detection groove one by one, the cover plate is closed, a large amount of time is wasted, the detection solution cannot be timely put into the sample detection groove, certain inaccurate bears exist in detection data, and the time for putting each detection solution into the sample detection groove is different, when the detection solution is detected on the machine, the detection time of each detection solution is different, so that the detected data is inaccurate, and the experimental result is influenced.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the internal structure of the present invention;
fig. 3 is an enlarged structural view of a portion a in fig. 1.
Reference symbol comparison table:
1. a housing; 2. a support frame; 3. a reaction box; 4. a stirring rod; 5. a filter screen; 6. a stirring motor; 7. an electromagnetic valve; 8. a communicating pipe; 9. a cover plate; 10. a drip nozzle; 11. a control panel; 12. a display screen; 13. a sample detection tank; 14. an electronic scale; 15. a laser; 16. a photoelectric receiving tube; 17. a power supply transmission module; 18. a signal conditioning module; 19. a main control module; 20. and (4) supporting the base.
Detailed Description
In order to make the content of the present invention more clearly understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. In which like parts are designated by like reference numerals. It should be noted that the terms "front," "back," "left," "right," "upper" and "lower" used in the following description refer to directions in the drawings, and the terms "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.
Example 1
As shown in fig. 1 and soil, a detection device for rapid detection of pesticide residue comprises: a housing 1.
A support frame 2 is arranged on one side above the shell 1, a reaction box 3 is arranged on the support frame 2, a stirring motor 6 is arranged at the bottom of the reaction box 3, a stirring rod 4 is arranged inside the reaction box 3, wherein the stirring rod 4 is connected with a rotating shaft of the stirring motor 6, a filter screen 5 is arranged inside the reaction box 3, the filter screen 5 is positioned above the stirring rod 4, an electromagnetic valve 7 is arranged on one side of the bottom of the reaction box 3, a communicating pipe 8 is arranged below the electromagnetic valve 7, the communicating pipe 8 is a hose, a control panel 11 is arranged on one side, away from the support frame 2, above the shell 1, a display screen 12 is arranged on the control panel 11, a detection placing groove 21 is arranged above the shell 1, the detection placing groove 21 is positioned between the support frame 2 and the control panel 11, and a sample detection groove 13 is arranged at the bottom of the detection placing groove 21, wherein, the inside of sample detection groove 13 is equipped with the cell, one side of detecting standing groove 21 is equipped with apron 9, be equipped with on the apron 9 and drip mouth 10, drip mouth 10 one end with communicating pipe 8 is kept away from the one end of reaction box 3 links to each other, the top of casing 1 is equipped with electronic scale 14, the inside of casing 1 is equipped with laser 15, the inside of casing 1 is equipped with photoelectric receiving tube 16, inside one side of casing 1 is equipped with power transformer module 17, keep away from inside of casing 1 one side of power transformer module 17 is equipped with host system 19, one side that the inside of casing 1 is close to host system 19 is equipped with signal conditioning module 18, the below of casing 1 is equipped with supporting seat 20.
Referring to fig. 2, the laser 15 and the photoelectric receiving tube 16 are correspondingly disposed at two sides of the sample detection groove 13, and after the cover plate 9 is closed, the drip nozzle 10 is located right above the sample detection groove 13.
It should be noted that, the display screen 12 is a touch screen, which facilitates touch control through the display screen 12, realizes interaction of a human-computer interface, and improves overall practicability. The drip nozzle 10 and the cover plate 9 can be detached from each other, and after the sample detection is completed, the drip nozzle 10 can be detached from the cover plate 9, so that the drip nozzle 10 can be cleaned and replaced conveniently.
The material of reaction box 3 is glass material, reaction box 3 with support frame 2 can be dismantled, and sample testing accomplishes the back, need tear reaction box 3 down from support frame 2, washs reaction box 3, prevents to detect next time and causes the mutual pollution of sample.
Example 2
The detection method applying the detection device for rapidly detecting the residual pesticide is characterized by comprising the following steps of:
step one, plugging a power supply, turning on a detection device for rapidly detecting residual pesticides, displaying a picture on a display screen, prompting operation according to the picture, starting self-detection, and about 1 min. And after the self-checking is finished, entering a standby detection state.
Step two, preparing a detection reagent:
1. buffer solution: adding 1 bag of buffer into 500mL of distilled water or purified water, stirring and dissolving to obtain phosphate buffer solution pH7.6, and storing at room temperature.
2. Color developing agent: dissolving 1 bottle of color developing agent in 25mL of buffer solution, and storing 100 μ L in a refrigerator at 4 deg.C when using.
3. Substrate: dissolving 1 bottle of substrate with 12.5mL of distilled water or purified water, and storing in a refrigerator at 4 deg.C with 100 μ L when in use; or dissolving 1 bottle of substrate in 2.5mL of distilled water or purified water, and storing at 4 deg.C in 20 μ L refrigerator.
4. Cholinesterase: the enzyme preparation can be directly used without preparation, and 100 μ L of the enzyme preparation can be stored in a refrigerator at 4 deg.C.
Note: the reagent manufacturers are different, and the preparation is subject to the instruction.
And step three, taking 2g of apple samples, cutting the apples into small blocks, putting the small blocks into a triangular flask, adding 10mL of buffer solution, oscillating for 1-2 min, pouring out the extracting solution, standing for 2min, and waiting for detection. If the extract is turbid or contains too much impurities, it can be filtered and then measured.
And step three, firstly, carrying out comparison test, adding 2.5mL of buffer solution into a reaction bottle, then respectively adding 100 mu L of enzyme solution and color developing agent, uniformly mixing, standing for reaction for 10min, adding 100 mu L or 20 mu L of substrate, shaking uniformly, immediately pouring into a cuvette, timely putting into a sample detection tank, closing a cover plate, adjusting a test interface of parameters to be detected through a display screen on a control panel, starting to carry out comparison through a comparison button on a touch display screen, finishing comparison after 3 min, taking out a comparison sample, and keeping the interface of a display unchanged. And then carrying out sample detection, namely firstly putting the cuvette into a sample detection groove, closing a cover plate, putting 2.5ml of extracting solution into a reaction box, then respectively adding 100 mu L of enzyme solution and color developing agent, uniformly mixing by a motor, standing for 10min, then adding 100 mu L or 20 mu L of substrate, uniformly mixing, immediately opening an electromagnetic valve, enabling the solution to enter the cuvette through a communicating pipe, passing through a dripping nozzle, detecting by a touch display screen, and displaying the absorbance increment delta alpha t) and the inhibition rate by the display screen after the detection is finished. And the data is automatically saved.
Example 3
The detection method applying the detection device for rapidly detecting the residual pesticide is characterized by comprising the following steps of:
step one, plugging a power supply, turning on a detection device for rapidly detecting residual pesticides, displaying a picture on a display screen, prompting operation according to the picture, starting self-detection, and about 1 min. And after the self-checking is finished, entering a standby detection state.
Step two, preparing a detection reagent:
1. buffer solution: adding 1 bag of buffer into 500mL of distilled water or purified water, stirring and dissolving to obtain phosphate buffer solution pH7.6, and storing at room temperature.
2. Color developing agent: dissolving 1 bottle of color developing agent in 25mL of buffer solution, and storing 100 μ L in a refrigerator at 4 deg.C when using.
3. Substrate: dissolving 1 bottle of substrate with 12.5mL of distilled water or purified water, and storing in a refrigerator at 4 deg.C with 100 μ L when in use; or dissolving 1 bottle of substrate in 2.5mL of distilled water or purified water, and storing at 4 deg.C in 20 μ L refrigerator.
4. Cholinesterase: the enzyme preparation can be directly used without preparation, and 100 μ L of the enzyme preparation can be stored in a refrigerator at 4 deg.C.
Note: the reagent manufacturers are different, and the preparation is subject to the instruction.
And step three, taking 2g of banana sample, cutting the banana into small pieces, putting the small pieces into a triangular flask, adding 10mL of buffer solution, oscillating for 1-2 min, pouring out the extracting solution, standing for 2min, and waiting for detection. If the extract is turbid or contains too much impurities, it can be filtered and then measured.
And step three, firstly, carrying out comparison test, adding 2.5mL of buffer solution into a reaction bottle, then respectively adding 100 mu L of enzyme solution and color developing agent, uniformly mixing, standing for reaction for 10min, adding 100 mu L or 20 mu L of substrate, shaking uniformly, immediately pouring into a cuvette, timely putting into a sample detection tank, closing a cover plate, adjusting a test interface of parameters to be detected through a display screen on a control panel, starting to carry out comparison through a comparison button on a touch display screen, finishing comparison after 3 min, taking out a comparison sample, and keeping the interface of a display unchanged. And then carrying out sample detection, namely firstly putting the cuvette into a sample detection groove, closing a cover plate, putting 2.5ml of extracting solution into a reaction box, then respectively adding 100 mu L of enzyme solution and color developing agent, uniformly mixing by a motor, standing for 10min, then adding 100 mu L or 20 mu L of substrate, uniformly mixing, immediately opening an electromagnetic valve, enabling the solution to enter the cuvette through a communicating pipe, passing through a dripping nozzle, detecting by a touch display screen, and displaying the absorbance increment delta alpha t) and the inhibition rate by the display screen after the detection is finished. And the data is automatically saved.
Example 4
The detection method applying the detection device for rapidly detecting the residual pesticide is characterized by comprising the following steps of:
step one, plugging a power supply, turning on a detection device for rapidly detecting residual pesticides, displaying a picture on a display screen, prompting operation according to the picture, starting self-detection, and about 1 min. And after the self-checking is finished, entering a standby detection state.
Step two, preparing a detection reagent:
1. buffer solution: adding 1 bag of buffer into 500mL of distilled water or purified water, stirring and dissolving to obtain phosphate buffer solution pH7.6, and storing at room temperature.
2. Color developing agent: dissolving 1 bottle of color developing agent in 25mL of buffer solution, and storing 100 μ L in a refrigerator at 4 deg.C when using.
3. Substrate: dissolving 1 bottle of substrate with 12.5mL of distilled water or purified water, and storing in a refrigerator at 4 deg.C with 100 μ L when in use; or dissolving 1 bottle of substrate in 2.5mL of distilled water or purified water, and storing at 4 deg.C in 20 μ L refrigerator.
4. Cholinesterase: the enzyme preparation can be directly used without preparation, and 100 μ L of the enzyme preparation can be stored in a refrigerator at 4 deg.C.
Note: the reagent manufacturers are different, and the preparation is subject to the instruction.
And step three, taking 2g of lettuce samples, cutting the leaf vegetables into fragments with the square of about 25px, putting the fragments into a triangular flask, adding 10mL of buffer solution, oscillating for 1-2 min, pouring out the extracting solution, standing for 2min, and waiting for detection. If the extract is turbid or contains too much impurities, it can be filtered and then measured.
And step three, firstly, carrying out comparison test, adding 2.5mL of buffer solution into a reaction bottle, then respectively adding 100 mu L of enzyme solution and color developing agent, uniformly mixing, standing for reaction for 10min, adding 100 mu L or 20 mu L of substrate, shaking uniformly, immediately pouring into a cuvette, timely putting into a sample detection tank, closing a cover plate, adjusting a test interface of parameters to be detected through a display screen on a control panel, starting to carry out comparison through a comparison button on a touch display screen, finishing comparison after 3 min, taking out a comparison sample, and keeping the interface of a display unchanged. And then carrying out sample detection, namely firstly putting the cuvette into a sample detection groove, closing a cover plate, putting 2.5ml of extracting solution into a reaction box, then respectively adding 100 mu L of enzyme solution and color developing agent, uniformly mixing by a motor, standing for 10min, then adding 100 mu L or 20 mu L of substrate, uniformly mixing, immediately opening an electromagnetic valve, enabling the solution to enter the cuvette through a communicating pipe, passing through a dripping nozzle, detecting by a touch display screen, and displaying the absorbance increment delta alpha t) and the inhibition rate by the display screen after the detection is finished. And the data is automatically saved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (9)
1. A detection device for residual pesticide short-term test, its characterized in that includes: the device comprises a shell (1), wherein a support frame (2) is arranged on one side above the shell (1), a reaction box (3) is arranged on the support frame (2), a stirring motor (6) is arranged at the bottom of the reaction box (3), a stirring rod (4) is arranged inside the reaction box (3), the stirring rod (4) is connected with a rotating shaft of the stirring motor (6), a filter screen (5) is arranged inside the reaction box (3), the filter screen (5) is positioned above the stirring rod (4), an electromagnetic valve (7) is arranged on one side of the bottom of the reaction box (3), a communicating pipe (8) is arranged below the electromagnetic valve (7), a control panel (11) is arranged on one side, far away from the support frame (2), of the top of the shell (1), a display screen (12) is arranged on the control panel (11), a detection placing groove (21) is arranged on the top of the shell (1), the detection placing groove (21) is positioned between the support frame (2) and the control panel (11), a sample detection groove (13) is arranged at the bottom of the detection placing groove (21), a cover plate (9) is arranged on one side of the detection placing groove (21), a drip nozzle (10) is arranged on the cover plate (9), one end of the drip nozzle (10) is connected with one end of the communicating pipe (8) far away from the reaction box (3), an electronic scale (14) is arranged above the shell (1), a laser (15) is arranged inside the shell (1), a photoelectric receiving pipe (16) is arranged inside the shell (1), a power supply transmission module (17) is arranged on one side of the inside of the shell (1), a main control module (19) is arranged on one side of the inside of the shell (1) far away from the power supply transmission module (17), and a signal conditioning module (18) is arranged on one side of the inside of the shell (1) close to the main control module (19), a supporting seat (20) is arranged below the shell (1).
2. The device for rapidly detecting pesticide residues as claimed in claim 1, wherein the sample detection tank (13) is internally provided with a cuvette.
3. The device for rapidly detecting pesticide residues as claimed in claim 2, wherein the laser (15) and the photoelectric receiving tube (16) are correspondingly arranged on two sides of the sample detection groove (13).
4. The device for rapidly detecting pesticide residue as claimed in claim 1, wherein the drip nozzle (10) is located right above the sample detection groove (13) after the cover plate (9) is closed.
5. The detection device for rapidly detecting pesticide residues as claimed in claim 1, wherein the display screen (12) is a touch screen.
6. The device for the rapid detection of pesticide residues as claimed in claim 1, characterized in that the drip nozzle (10) and the cover plate (9) are detachable.
7. The device for rapidly detecting pesticide residues as claimed in claim 1, wherein the communicating pipe (8) is a hose.
8. The device for rapidly detecting pesticide residues as claimed in claim 1, wherein the reaction box (3) is made of glass material, and the reaction box (3) and the support frame (2) are detachable.
9. The detection method using the detection device for rapidly detecting the pesticide residue as claimed in any one of claims 1 to 8, characterized by comprising the following steps:
step one, plugging a power supply, turning on a detection device for rapidly detecting residual pesticides, displaying a picture on a display screen, prompting operation according to the picture, starting self-detection, and about 1 min. And after the self-checking is finished, entering a standby detection state.
Step two, preparing a detection reagent:
1. buffer solution: adding 1 bag of buffer into 500mL of distilled water or purified water, stirring and dissolving to obtain phosphate buffer (pH7.6), and storing at room temperature.
2. Color developing agent: dissolving 1 bottle of color developing agent in 25mL of buffer solution, and storing 100 μ L in a refrigerator at 4 deg.C when using.
3. Substrate: dissolving 1 bottle of substrate with 12.5mL of distilled water or purified water, and storing in a refrigerator at 4 deg.C with 100 μ L when in use; or dissolving 1 bottle of substrate in 2.5mL of distilled water or purified water, and storing at 4 deg.C in 20 μ L refrigerator.
4. Cholinesterase: the enzyme preparation can be directly used without preparation, and 100 μ L of the enzyme preparation can be stored in a refrigerator at 4 deg.C.
Note: the reagent manufacturers are different, and the preparation is subject to the instruction.
And step three, taking 2g of fruit and vegetable samples (4 g of tubers), cutting the leaf vegetables into fragments with the square size of about 25px, taking the cross section samples or the skins of the tubers, putting the tubers into a triangular flask, adding 10mL of buffer solution, oscillating for 1-2 min, pouring out the extracting solution, standing for 2min, and waiting for detection. If the extract is turbid or contains too much impurities, it can be filtered and then measured.
And step three, firstly, carrying out comparison test, adding 2.5mL of buffer solution into a reaction bottle, then respectively adding 100 mu L of enzyme solution and color developing agent, uniformly mixing, standing for reaction for 10min, adding 100 mu L (or 20 mu L) of substrate, shaking uniformly, immediately pouring into a cuvette, timely putting into a sample detection groove, closing a cover plate, adjusting a test interface of parameters to be detected through a display screen on a control panel, starting to perform comparison through a comparison button on a touch display screen, finishing comparison after 3 min, taking out a comparison sample, and keeping the interface of a display unchanged. And then carrying out sample detection, namely firstly putting the cuvette into a sample detection groove, closing a cover plate, putting 2.5ml of extracting solution into a reaction box, then respectively adding 100 mu L of enzyme solution and color developing agent, uniformly mixing by a motor, standing for 10min, then adding 100 mu L (or 20 mu L) of substrate, uniformly mixing, immediately opening an electromagnetic valve, enabling the solution to enter the cuvette through a communicating pipe and pass through a dripping nozzle, detecting by a touch display screen, and displaying the absorbance increment (delta alpha t) and the inhibition rate by the display screen after the detection is finished. And the data is automatically saved.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210023426.3A CN114354592B (en) | 2022-01-10 | 2022-01-10 | Detection device and detection method for rapid detection of residual pesticide |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210023426.3A CN114354592B (en) | 2022-01-10 | 2022-01-10 | Detection device and detection method for rapid detection of residual pesticide |
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| CN114354592A true CN114354592A (en) | 2022-04-15 |
| CN114354592B CN114354592B (en) | 2023-11-21 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116465880B (en) * | 2023-03-13 | 2023-11-10 | 广东加减乘除食品有限公司 | Food detection method |
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| CN114354592B (en) | 2023-11-21 |
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