CN114350505A - Preparation method and fermentation device of gamma-aminobutyric acid - Google Patents
Preparation method and fermentation device of gamma-aminobutyric acid Download PDFInfo
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- CN114350505A CN114350505A CN202210115208.2A CN202210115208A CN114350505A CN 114350505 A CN114350505 A CN 114350505A CN 202210115208 A CN202210115208 A CN 202210115208A CN 114350505 A CN114350505 A CN 114350505A
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Abstract
The invention discloses a preparation method of gamma-aminobutyric acid and a fermentation device thereof, relating to the technical field of preparation of gamma-aminobutyric acid, wherein the method comprises the following steps: inoculating the cultured gamma-aminobutyric acid production strain into a fermentation medium, putting the fermentation medium into a fermentation device, and performing aerobic and anaerobic two-stage combined fermentation, wherein the first stage is an aerobic fermentation stage, when the cell concentration reaches 15-30, the fermentation is switched into a second stage for anaerobic fermentation, and the anaerobic culture is performed until the fermentation is finished to obtain the gamma-aminobutyric acid; the fermentation device comprises a tank body and a stirring mechanism for promoting fermentation of a fermentation product; the stirring mechanism includes: the gas injection device comprises a rotating assembly, a deflection assembly and a gas injection assembly. According to the invention, on the premise of not adding the number of the stirring rods, the stirring area is increased, and the resistance during stirring, namely the resistance borne by the stirring rods during stirring, is reduced.
Description
Technical Field
The invention relates to the technical field of preparation of gamma-aminobutyric acid, in particular to a preparation method of gamma-aminobutyric acid and a fermentation device thereof.
Background
Gamma-aminobutyric acid (GABA for short), 4-aminobutyric acid and gamma-aminobutyric acid, and the molecular formula is C4H9NO2Relative molecular weight 103.2, is a white or near-white crystalline powder, very soluble in water, slightly soluble in hot ethanol, insoluble in cold ethanol, benzene, ether, melting point (mp): 203 deg.C and 204 deg.C (decomposition), melting and decomposing into pyrrolidone and water.
Because of the low natural abundance of GABA, which is difficult to extract and isolate in large quantities from some natural tissues, current methods for obtaining GABA are both chemical synthesis and biological methods, including both enrichment in plants and microbial fermentation. The microbial fermentation method is characterized in that L-sodium glutamate is used as a conversion substrate, a fermentation medium is formed by adding a carbon source, a nitrogen source and inorganic salt, and gamma-aminobutyric acid is prepared by converting a gamma-aminobutyric acid production strain. In contrast, the chemical synthesis has poor safety, the GABA content of the plant is low, and the GABA synthesized by microbial fermentation has development prospect.
The whole fermentation process of synthesizing GABA by adopting microbial fermentation is in a fermentation tank, so the fermentation tank plays a vital role in the whole fermentation process, but the existing fermentation tank adopts a standard mechanical stirring ventilation fermentation tank consistently for decades, and a plurality of factories and even continue to use a pure runoff stirring system and a tubular distributor, so that a fermented product cannot be in good contact with air in the fermentation process, the stirring effect is poor, and the whole fermentation efficiency can be influenced.
Disclosure of Invention
The invention aims to: provides a preparation method of gamma-aminobutyric acid and a fermentation device thereof, aiming at solving the problems that the fermentation product cannot be well contacted with the air, the stirring effect is poor and the whole fermentation efficiency is influenced in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme: the invention provides a fermentation device of gamma-aminobutyric acid, which comprises a tank body and a stirring mechanism for promoting fermentation of a fermentation product; the stirring mechanism includes: the rotating assembly comprises a stirring shaft coaxially arranged in the tank body; the stirring shaft can circumferentially rotate at the position; a gas injection channel is formed in the hollow part of the stirring shaft, and movable grooves are uniformly arranged on the side wall of the stirring shaft in a penetrating manner along the circumferential direction; the deflection component comprises stirring rods uniformly arranged on the outer side of the stirring shaft along the circumferential direction and a movable plate axially and slidably arranged in the gas injection channel; the stirring rod comprises a clamping part clamped in the movable groove and a stirring part; the clamping part is in movable sealing fit with the movable groove and can only rotate on the radial plane of the stirring shaft; the movable plate is connected with the clamping part through a transmission part, and the clamping part can be driven to rotate when the movable plate moves axially; the deflection assembly further comprises a first return spring capable of providing damping when the movable plate moves axially; and the gas injection assembly is used for intermittently injecting gas into the interior of the gas injection channel.
Further, joint portion is cylindricly, and stirring portion is shaft-like, the activity groove includes the first binding face with the global laminating of joint portion to and the second binding face with the terminal surface laminating of joint portion.
Further, the driving medium includes the hinge bar, the hinge bar is articulated to be set up joint portion is located the inside terminal surface of gas injection passageway to the other end extension of hinge bar is articulated with fly leaf top.
Further, the inside cavity of puddler, wherein, the inside of joint portion is formed with the cylindricality chamber, and the lateral wall of joint portion is provided with the inlet port with cylindricality chamber intercommunication, the inside of stirring portion is formed with the bar chamber, and stirring portion is close to (mixing) shaft one side and evenly is provided with the micropore of giving vent to anger with bar chamber intercommunication.
Furthermore, one side of the stirring part close to the stirring shaft is matched with the outer wall of the stirring shaft.
Further, the top end of the stirring shaft extends to the top of the tank body and is connected with a driving source.
Further, the gas injection assembly comprises a gas generating piece and a driving piece; the gas generating piece comprises a negative pressure box fixed at the top of the tank body, a piston horizontally arranged in the negative pressure box in a sliding manner, a movable rod vertically arranged on one side of the piston close to the stirring shaft, and a gas inlet pipe and a gas outlet pipe which are arranged at one end of the negative pressure box far away from the stirring shaft; one end of the movable rod, which is far away from the piston, extends to one end of the negative pressure box and is fixedly connected with a pressing plate, a first one-way valve and a second one-way valve are respectively arranged on the air inlet pipe and the air outlet pipe, one end of the air outlet pipe, which is far away from the negative pressure box, is connected with the stirring shaft through a rotary joint, and the air outlet pipe is connected with the gas injection channel; the driving piece is used for driving the pressing plate to move left and right in the horizontal direction.
Further, the driving piece includes the cam of fixing on the (mixing) shaft, and when the (mixing) shaft rotated, the cam can promote the pressure board to removing to being close to negative-pressure tank one side, the driving piece still includes and is providing damped second reset spring when the pressure board is close to negative-pressure tank one side and removes.
Further, the air exhaust mechanism is further included and used for exhausting air inside the tank body; exhaust mechanism is including separating a section of thick bamboo, separate a section of thick bamboo setting at gas injection passageway top to cut apart the gas injection passageway and be formed with annular exhaust chamber, the one end in annular exhaust chamber is located a jar internal portion, and the other end in annular exhaust chamber is located a jar body top, evenly is provided with first exhaust hole on the (mixing) shaft that is located jar internal annular chamber one side, evenly is provided with the second exhaust hole on the (mixing) shaft that is located the annular chamber one side at jar body top to be provided with the shutoff piece on the second exhaust hole, the shutoff piece is including the shutoff ball that is located second exhaust hole department to and provide damped third reset spring when the shutoff ball removes to keeping away from exhaust hole one side.
The invention also provides a preparation method of the gamma-aminobutyric acid based on the fermentation device of the gamma-aminobutyric acid, which comprises the following steps: inoculating the cultured gamma-aminobutyric acid production strain into a fermentation medium, introducing the gamma-aminobutyric acid production strain into a tank body 1, and performing aerobic and anaerobic two-stage combined fermentation, wherein the first stage is an aerobic fermentation stage, and after controlling the pH value to be 5.5-6.0, starting a driving source to enable a stirring shaft 2 in a rotating assembly to rotate to drive a stirring rod 2 in a deflection assembly to rotate circumferentially to stir the fermented product; and injecting gas into the gas injection channel in a clearance mode by the gas injection assembly to drive the stirring rod 2 in the deflection assembly to deflect up and down and exhaust gas, in the process, maintaining the dissolved oxygen concentration at 60-90%, controlling the temperature at 30-32 ℃, performing aerobic culture for 10-20 hours, performing second-stage anaerobic fermentation when the cell concentration reaches 15-30, closing the driving source to seal the interior of the tank body 1, performing anaerobic culture until the fermentation is finished, controlling the temperature at 35-38 ℃ in the anaerobic stage, and performing fermentation for 40-50 hours to obtain the gamma-aminobutyric acid.
Compared with the prior art, the above one or more technical schemes have the following beneficial effects:
1. the fermentation technology adopts aerobic and anaerobic two-stage combined fermentation, the temperature is controlled in a segmented manner in the fermentation process, and a segmented regulation and control means is adopted on the demand of oxygen, so that the gamma-aminobutyric acid production strain is metabolized to produce gamma-aminobutyric acid in an anaerobic state, the segmented ventilation and high-temperature fermentation are realized, the fermentation period is shortened, the consumption of cooling water is reduced, the cost is reduced, and the yield of the gamma-aminobutyric acid is improved.
2. According to the invention, on the premise of not adding the number of the stirring rods, the stirring area is increased, and the resistance during stirring, namely the resistance borne by the stirring rods during stirring, is reduced.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
In the drawings:
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of a half-section structure of the present invention;
FIG. 3 is a schematic view of a portion of the structure of FIG. 1 at A;
FIG. 4 is a schematic view of a portion of the structure of FIG. 2 at B;
FIG. 5 is a schematic view of a portion of the structure of FIG. 2 at C;
FIG. 6 is another structural diagram of FIG. 5;
FIG. 7 is a schematic view of a portion of the structure of FIG. 2 at D;
fig. 8 is a schematic view of a half-section partial structure of the movable groove of the present invention.
Detailed Description
In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It should be understood that the data so used may be interchanged under appropriate circumstances such that embodiments of the application described herein may be used. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
In this application, the terms "upper", "lower", "left", "right", "front", "rear", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings. These terms are used primarily to better describe the present application and its embodiments, and are not used to limit the indicated devices, elements or components to a particular orientation or to be constructed and operated in a particular orientation.
Moreover, some of the above terms may be used to indicate other meanings besides the orientation or positional relationship, for example, the term "on" may also be used to indicate some kind of attachment or connection relationship in some cases. The specific meaning of these terms in this application will be understood by those of ordinary skill in the art as appropriate.
Furthermore, the terms "mounted," "disposed," "provided," "connected," and "sleeved" are to be construed broadly. For example, it may be a fixed connection, a removable connection, or a unitary construction; can be a mechanical connection, or an electrical connection; may be directly connected, or indirectly connected through intervening media, or may be in internal communication between two devices, elements or components. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.
Referring to fig. 1 to 8, the present invention provides a fermentation apparatus for gamma-aminobutyric acid, which includes a tank body 1, wherein a temperature control mechanism is installed on the tank body 1, the temperature control mechanism includes a controller (not shown), a temperature sensor 34 and a heating element, the temperature sensor 34 detects the temperature of a fermented product in the tank body 1 in real time and transmits a signal to the controller, the controller controls the heating element to heat the fermented product in the tank body 1, when the temperature reaches a preset value, the preset value can be adjusted by the controller, for example, when aerobic fermentation is performed, the preset value is 31 ℃, and when anaerobic fermentation is performed, the preset value is 37 ℃, the controller controls the heating element to stop heating.
The heating assembly includes a water jacket layer sleeved on the lower portion of the tank body and a heating pipe (not shown) installed inside the water jacket layer. The heating pipe heats the water in the water jacket layer, and then the tank body 1 is heated by the water, so that the tank body 1 is heated uniformly, and the fermentation efficiency of aerobic and anaerobic stage fermentation is improved.
The top of the jar body 1 is provided with the inlet pipe, and the bottom of the jar body 1 is provided with the discharging pipe, and all installs the valve on inlet pipe and the discharging pipe, and level sensor 35 is still installed to the inside of the jar body 1. When the liquid level in the tank body 1 reaches a preset value (the preset value is a value of the liquid level larger than the height value of the movable groove), the liquid level sensor 35 transmits a liquid level signal to the controller, and the controller can control the material injection mechanism externally connected to the feeding pipe to stop material conveying.
The fermentation device also comprises a stirring mechanism for promoting the fermentation of the fermentation product; the rabbling mechanism includes: the rotating assembly comprises a stirring shaft 2 coaxially arranged in the tank body 1; the stirring shaft 2 can circumferentially rotate at the position; a gas injection channel 3 is formed in the hollow part of the stirring shaft 2, and the side wall of the stirring shaft 2 is uniformly provided with movable grooves 4 along the circumferential direction in a penetrating way; the deflection component comprises stirring rods 5 which are uniformly arranged on the outer side of the stirring shaft 2 along the circumferential direction, and a movable plate 6 which is axially arranged in the gas injection channel 3 in a sliding manner; the stirring rod 5 comprises a clamping part 7 and a stirring part 8 which are clamped in the movable groove 4; the clamping part 7 is in movable sealing fit with the movable groove 4 and can only rotate on the radial plane of the stirring shaft 2; the movable plate 6 is connected with the clamping part through a transmission part, and the clamping part 7 can be driven to rotate when the movable plate 6 moves axially; the yaw assembly further comprises a first return spring 9 capable of providing damping when the movable plate 6 moves axially; and a gas injection assembly for intermittently injecting gas into the interior of the gas injection channel 3.
Specifically, the top end of the stirring shaft 2 extends to the top of the tank body 1 and is connected with a driving source. The drive source is a motor that is connected to the agitator shaft by a chain structure (not shown).
Specifically, joint portion 7 is cylindricly, and stirring portion 8 is shaft-like, and movable groove 4 includes the first binding face 10 with the global laminating of joint portion 7 to and the second binding face 11 with the terminal surface laminating of joint portion 7, all be provided with the sealing layer on second binding face 11 and the first binding face 10. With this structure, the engaging portion 7 can be sealed while the movable groove 4 is rotated.
More specifically, the driving medium includes a hinge rod 12, the hinge rod 12 is hinged to the end surface of the clamping portion 7 located inside the gas injection channel 3, and the other end of the hinge rod 12 extends to be hinged to the top of the movable plate 6. According to the structure, when the movable plate 6 moves axially downwards, the hinged part of the clamping part 7 and the hinged rod 12 can be driven to move downwards through the hinged rod 12, and when the movable plate 6 moves axially upwards, the hinged part of the clamping part 7 and the hinged rod 12 can be driven to move upwards through the hinged rod 12.
More specifically, the inside cavity of puddler 5, wherein, the inside of joint portion 7 is formed with cylindricality chamber 13, and the lateral wall of joint portion 7 is provided with the inlet port 14 with cylindricality chamber 13 intercommunication, and the inside of stirring portion 8 is formed with bar chamber 15, and stirring portion 8 is close to (mixing) shaft 2 one side and evenly is provided with the micropore 16 of giving vent to anger that communicates with bar chamber 15.
During application, in the aerobic fermentation process, the stirring shaft 2 needs to rotate and gas needs to be introduced into the tank body 1 to improve the oxygen content, during specific operation, the stirring shaft 2 is driven to rotate circumferentially by the driving source, and gas is injected into a gap in a gas injection channel of the stirring shaft 2 through the gas injection assembly; the process of intermittently injecting gas into the gas injection channel 3 of the stirring shaft 2 by the gas injection assembly is divided into two stages, namely a gas injection stage and a gas injection stopping stage; during the gas injection phase, the gas pressure inside the gas injection channel 3 is continuously increased, so that the movable plate 6 moves downwards and compresses the first return spring 9; when the movable plate 6 moves downwards, one end of the hinge rod 12 is pulled to move downwards, and then the other end of the hinge rod 12 pulls the clamping portion 7 to enable the clamping portion 7 to rotate in the movable groove 4, so that the stirring portion 8 swings upwards; when the air inlet 14 moves into the air injection channel 3 along with the continuous rotation of the clamping part 7, the air injection channel 3 is communicated with the cylindrical cavity 13 of the clamping part 7, air in the air injection channel 3 can enter the cylindrical cavity 13 and the strip-shaped cavity 15 through the air inlet 14, and then is quickly discharged through the air outlet micropores 16 of the strip-shaped cavity 15 to enter a fermented product, so that ventilation is completed; in the process of stopping the gas injection stage, the gas pressure in the gas injection channel 3 is reduced, the movable plate 6 moves upwards under the action of the first return spring 9 and can push the clamping part 7 to rotate reversely through the hinge rod 12, so that the clamping part 7 drives the stirring part 8 to swing downwards; thus, in combination with the circumferential rotation of the stirring shaft 2 and the gap gas injection effect of the gas injection assembly, the movement track of the stirring part 8 is circumferential rotation and simultaneously swings up and down in the whole process, compared with a stirring mechanism in the prior art, the stirring mechanism improves the stirring area and reduces the resistance during stirring (namely, the resistance borne by the stirring rod during stirring) on the premise of not adding the quantity of the stirring rods 5, and the stirring mechanism not only can stir transversely, but also can stir longitudinally, so that the contact area of a fermented product and air is improved, and the fermentation efficiency in the aerobic process is improved.
Further, the gas injection assembly comprises a gas generating piece and a driving piece; the gas generating piece comprises a negative pressure box 17 fixed on the top of the tank body 1, a piston 18 horizontally arranged in the negative pressure box 17 in a sliding manner, a movable rod 19 vertically arranged on one side of the piston 18 close to the stirring shaft 2, and a gas inlet pipe 20 and a gas outlet pipe 21 arranged on one end of the negative pressure box 17 far away from the stirring shaft 2; one end of the movable rod 19, which is far away from the piston 18, extends to one end of the negative pressure box 17 and is fixedly connected with a pressing plate 22, a first one-way valve 23 and a second one-way valve 24 are respectively arranged on the air inlet pipe 20 and the air outlet pipe 21, one end of the air outlet pipe 21, which is far away from the negative pressure box 17, is connected with the stirring shaft 2 through a rotary joint 25, and the air outlet pipe 21 is connected with the gas injection channel 3; the driving member is used for driving the pressing plate 22 to move left and right in the horizontal direction. Through the structure, the driving part drives the pressing plate 22 to move left and right in the horizontal direction, so that the pressing plate 22 can drive the piston 18 to continuously move in the negative pressure box 17 through the movable rod 19, and further, the inside of the negative pressure box 17 continuously generates positive pressure and negative pressure, when the positive pressure is generated, the gas in the negative pressure box 17 can enter the gas injection channel 3 through the gas outlet pipe 21, when the negative pressure is generated, the air can be supplemented into the negative pressure box 17 through the gas inlet pipe 21, the gas inlet pipe 21 can be connected with an oxygen generator, and when the negative pressure box generates the positive pressure, namely a gas injection stage, when the negative pressure box generates the negative pressure, namely the gas injection stage is stopped; the time generated by the negative pressure is utilized to enable the clamping part 7 to drive the stirring part 8 to swing downwards.
Specifically, the driving member includes a cam 26 fixed on the stirring shaft 2, when the stirring shaft 2 rotates, the cam 26 can push the pressing plate 22 to move towards the side close to the negative pressure box 17, and the driving member further includes a second return spring 27 for providing damping when the negative pressure box 17 moves towards the negative pressure side. Through the structure, the rotation of (mixing) shaft 2 drives cam 26 and rotates, and then drives and constantly removes to being close to negative-pressure tank 17 one side according to pressing board 22, thereby make negative-pressure tank 17 inside constantly produce malleation and negative pressure, with the leading-in gas injection passageway 3 of gas gap formula, it needs to notice that, whole cooperation structure, by the drive of a motor, the motor is when driving (mixing) shaft 2 pivoted, makes the gas gap formula get into inside gas injection passageway 3.
Specifically, the fermentation device for gamma-aminobutyric acid further comprises an exhaust mechanism, and the exhaust mechanism is used for exhausting air in the tank body 1.
The exhaust mechanism comprises a separating cylinder 28, the separating cylinder 28 is arranged at the top of the gas injection channel 3, so as to isolate the gas injection channel 3 to form an annular exhaust cavity 29, one end of the annular exhaust cavity 29 is positioned in the tank body 1, the other end of the annular exhaust cavity 29 is positioned at the top of the tank body 1, a first exhaust hole 30 is uniformly arranged on a stirring shaft 2 positioned at one side of an annular cavity in the tank body 1, a second exhaust hole 31 is uniformly arranged on the stirring shaft 2 positioned at one side of the annular cavity at the top of the tank body 1, a plugging piece is arranged on the second exhaust hole 31 and comprises a plugging ball 32 positioned at the second exhaust hole 31, and a third return spring 33 for providing damping when the plugging ball 32 moves to one side far away from the second exhaust hole 31. Along with the increase of the air pressure in the tank body 1, the gas pushes the blocking ball 32 to be far away from the second vent hole 31, the blocking of the second vent hole 31 is removed, and the gas can be discharged through the second vent hole 31.
Specifically, one side of the stirring part 8 close to the stirring shaft 2 is matched with the outer wall of the stirring shaft 2. This upper structure, when the reaction need not the stirring, stirring portion 8 of puddler 12 can laminate on (mixing) shaft 2 outer wall this moment, micropore 16 of will giving vent to anger carries out the jam in anaerobic fermentation process, need not the stirring and ventilates, only need close the driving source this moment, the (mixing) shaft 2 stall stops the gas injection with the gas injection subassembly, stirring portion 8 of puddler 12 can laminate on (mixing) shaft 2 outer wall this moment, can let the fermentation that the zymolite can be better ferment, at this in-process, exhaust mechanism's shutoff ball 32 shutoff second exhaust hole 31, the inlet port 14 of joint portion 7 is blockked up by first binding face 10, the inside formation encapsulated situation of jar body 1.
The invention also provides a preparation method of the gamma-aminobutyric acid based on the fermentation device of the gamma-aminobutyric acid, which comprises the following steps: inoculating the cultured gamma-aminobutyric acid production strain into a fermentation medium, introducing the gamma-aminobutyric acid production strain into a tank body 1, and performing aerobic and anaerobic two-stage combined fermentation, wherein the first stage is an aerobic fermentation stage, and after controlling the pH value to be 5.5-6.0, starting a driving source to enable a stirring shaft 2 in a rotating assembly to rotate to drive a stirring rod 2 in a deflection assembly to rotate circumferentially to stir the fermented product; and injecting gas into the gas injection channel in a clearance mode by the gas injection assembly to drive the stirring rod 2 in the deflection assembly to deflect up and down and exhaust gas, in the process, maintaining the dissolved oxygen concentration at 60-90%, controlling the temperature at 30-32 ℃, performing aerobic culture for 10-20 hours, performing second-stage anaerobic fermentation when the cell concentration reaches 15-30, closing the driving source to seal the interior of the tank body 1, performing anaerobic culture until the fermentation is finished, controlling the temperature at 35-38 ℃ in the anaerobic stage, and performing fermentation for 40-50 hours to obtain the gamma-aminobutyric acid.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A fermentation device of gamma-aminobutyric acid comprises a tank body and is characterized by also comprising a stirring mechanism for promoting fermentation of a fermentation product; the stirring mechanism includes: the rotating assembly comprises a stirring shaft coaxially arranged in the tank body; the stirring shaft can circumferentially rotate at the position; a gas injection channel is formed in the hollow part of the stirring shaft, and movable grooves are uniformly arranged on the side wall of the stirring shaft in a penetrating manner along the circumferential direction; the deflection component comprises stirring rods uniformly arranged on the outer side of the stirring shaft along the circumferential direction and a movable plate axially and slidably arranged in the gas injection channel; the stirring rod comprises a clamping part clamped in the movable groove and a stirring part; the clamping part is in movable sealing fit with the movable groove and can only rotate on the radial plane of the stirring shaft; the movable plate is connected with the clamping part through a transmission part, and the clamping part can be driven to rotate when the movable plate moves axially; the deflection assembly further comprises a first return spring capable of providing damping when the movable plate moves axially;
and the gas injection assembly is used for intermittently injecting gas into the interior of the gas injection channel.
2. The fermentation apparatus of gamma-aminobutyric acid according to claim 1, wherein the engaging portion is cylindrical, the stirring portion is rod-shaped, and the movable groove comprises a first engaging surface engaging with a circumferential surface of the engaging portion and a second engaging surface engaging with an end surface of the engaging portion.
3. The fermentation apparatus of gamma-aminobutyric acid according to claim 2, wherein the driving member comprises a hinge rod, the hinge rod is hinged to an end surface of the clamping portion inside the gas injection channel, and the other end of the hinge rod extends to hinge with the top of the movable plate.
4. The fermentation device of gamma-aminobutyric acid according to claim 3, wherein the stirring rod is hollow, wherein a cylindrical cavity is formed inside the clamping portion, an air inlet communicated with the cylindrical cavity is formed on a side wall of the clamping portion, a strip-shaped cavity is formed inside the stirring portion, and air outlet micropores communicated with the strip-shaped cavity are uniformly formed on one side of the stirring portion close to the stirring shaft.
5. The fermentation apparatus of gamma-aminobutyric acid according to claim 4, wherein a side of the stirring part near the stirring shaft is adapted to an outer wall of the stirring shaft.
6. The fermentation apparatus of gamma-aminobutyric acid according to claim 1, wherein a driving source is connected to a top end of the stirring shaft extending to a top of the tank.
7. The apparatus of claim 5, wherein the gas injection assembly comprises a gas generating member and a driving member; the gas generating piece comprises a negative pressure box fixed at the top of the tank body, a piston horizontally arranged in the negative pressure box in a sliding manner, a movable rod vertically arranged on one side of the piston close to the stirring shaft, and a gas inlet pipe and a gas outlet pipe which are arranged at one end of the negative pressure box far away from the stirring shaft; one end of the movable rod, which is far away from the piston, extends to one end of the negative pressure box and is fixedly connected with a pressing plate, a first one-way valve and a second one-way valve are respectively arranged on the air inlet pipe and the air outlet pipe, one end of the air outlet pipe, which is far away from the negative pressure box, is connected with the stirring shaft through a rotary joint, and the air outlet pipe is connected with the gas injection channel; the driving piece is used for driving the pressing plate to move left and right in the horizontal direction.
8. The apparatus of claim 7, wherein the driving member comprises a cam fixed to the stirring shaft, the cam being capable of pushing the pressing plate to move toward the side close to the negative pressure tank when the stirring shaft rotates, and the driving member further comprises a second return spring for providing damping when the pressing plate moves toward the side close to the negative pressure tank.
9. The apparatus for fermenting gamma-aminobutyric acid according to claim 8, further comprising an air exhaust mechanism for exhausting air inside the tank; exhaust mechanism is including separating a section of thick bamboo, separate a section of thick bamboo setting at gas injection passageway top to cut apart the gas injection passageway and be formed with annular exhaust chamber, the one end in annular exhaust chamber is located a jar internal portion, and the other end in annular exhaust chamber is located a jar body top, evenly is provided with first exhaust hole on the (mixing) shaft that is located jar internal annular chamber one side, evenly is provided with the second exhaust hole on the (mixing) shaft that is located the annular chamber one side at jar body top to be provided with the shutoff piece on the second exhaust hole, the shutoff piece is including the shutoff ball that is located second exhaust hole department to and provide damped third reset spring when the shutoff ball removes to keeping away from exhaust hole one side.
10. A method for producing gamma-aminobutyric acid based on the fermentation device of gamma-aminobutyric acid according to claim 9, comprising the steps of: inoculating the cultured gamma-aminobutyric acid production strain into a fermentation medium, introducing the gamma-aminobutyric acid production strain into a tank body 1, and performing aerobic and anaerobic two-stage combined fermentation, wherein the first stage is an aerobic fermentation stage, and after controlling the pH value to be 5.5-6.0, starting a driving source to enable a stirring shaft 2 in a rotating assembly to rotate to drive a stirring rod 2 in a deflection assembly to rotate circumferentially to stir the fermented product; and injecting gas into the gas injection channel in a clearance mode by the gas injection assembly to drive the stirring rod 2 in the deflection assembly to deflect up and down and exhaust gas, in the process, maintaining the dissolved oxygen concentration at 60-90%, controlling the temperature at 30-32 ℃, performing aerobic culture for 10-20 hours, performing second-stage anaerobic fermentation when the cell concentration reaches 15-30, closing the driving source to seal the interior of the tank body 1, performing anaerobic culture until the fermentation is finished, controlling the temperature at 35-38 ℃ in the anaerobic stage, and performing fermentation for 40-50 hours to obtain the gamma-aminobutyric acid.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115921332A (en) * | 2022-11-04 | 2023-04-07 | 福建中环宝科技集团有限公司 | Equipment for capturing volatile CO2 in garbage |
| CN117844610A (en) * | 2023-12-31 | 2024-04-09 | 枣庄市杰诺生物酶有限公司 | Preparation device and method of high-purity aminobutyric acid |
-
2022
- 2022-02-05 CN CN202210115208.2A patent/CN114350505A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115921332A (en) * | 2022-11-04 | 2023-04-07 | 福建中环宝科技集团有限公司 | Equipment for capturing volatile CO2 in garbage |
| CN117844610A (en) * | 2023-12-31 | 2024-04-09 | 枣庄市杰诺生物酶有限公司 | Preparation device and method of high-purity aminobutyric acid |
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Application publication date: 20220415 |