CN101130791B - Device and method for solid-state fermentation and preparation of alcohol using sorgo stalk - Google Patents
Device and method for solid-state fermentation and preparation of alcohol using sorgo stalk Download PDFInfo
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- CN101130791B CN101130791B CN200610112613A CN200610112613A CN101130791B CN 101130791 B CN101130791 B CN 101130791B CN 200610112613 A CN200610112613 A CN 200610112613A CN 200610112613 A CN200610112613 A CN 200610112613A CN 101130791 B CN101130791 B CN 101130791B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
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Abstract
The invention relates to a device and a method for solid-state fermenting alcohol with sweet Chinese sorghum straw. The device comprises the following parts: an inside spin propeller, a fermentation tank of air seal machinery on the casing, wherein the lying of the tank are communicated with air lifting tank vertically and are arranged in one piece, the stepping motor is electric connected with spin propeller in the fermentation tank, the lower port of the air lifting tank is connected with cylinder and compressor by gas line, the compressor is communicated with air collector by the conduct pipe, the upper port of air lifting tank is connected with the lower ports of the active carbon absorption columns, the conduct pipe is provided with alcohol concentration measuring instrument and change-over valve, the upper port of the active carbon absorption column is connected with the air collector by the pipe line, the lower port of the active carbon absorption column is connected with air pump separately, the upper port is connected with condenser by the air pipe, the condenser is communicated with acceptor. The method applies disintegration, disinfection, inoculation, activating, fermenting, air lifting and absorption, condensation and recovering, and the alcohol production completed in the device reduces the procedure.
Description
Technical field
The present invention relates to a kind of technology of utilizing continuous solid-state fermentation device fabrication alcohol, particularly relate to a kind of technology of utilizing sweet sorghum stalk solid state fermentation alcohol.
Background technology
Increase along with the demand for fuel amount, rising steadily of gas price, too high with grain-production ethanol cost, and the price that is used for substrate such as the raw materials such as molasses of fermentative production alcohol constantly rises, we must seek a kind of cheap material and effective production technique, to reduce the cost of biofuel.Wherein sweet sorghum is exactly a kind of raw material of very potential production alcohol.Sweet sorghum is also referred to as sugared Chinese sorghum, is the high energy crop in the biomass energy, because of its characteristic with drought resisting, waterlogging, salt tolerant alkali, the good reputation of " camel in the crop " is arranged again, is the hope of future source of energy.Mainly contain sucrose, glucose, fructose in the stem juice composition of sweet sorghum, and in the majority with sucrose, for fermentative production alcohol provides abundant sugar source.
Existing liquid state fermentation technology is the juice that squeezes earlier in the sweet sorghum stalk, carries out liquid state fermentation alcohol with juice then.But squeezing the juice with sweet sorghum exists certain problem, and sweet sorghum stalk contains so high moisture and sugar unlike sugarcane, has a large amount of sugar loss in the process of squeezing the juice.When squeezing the juice fermentation in addition, expressed mixing juice must leach the Chinese sorghum slag, even distill enrichment step, complex process in addition through flocculation filtration.Must carry out the work of treatment of waste water after the last fermentation ends, be again a huge expense.
The technology that existing solid state fermentation is produced alcohol is in the conventional mode, and inoculation is earlier fermented in fermentation vat then, and will ferment after the end expects to pack into again distills in the still kettle, obtains alcohol product.Need the transfer several times through material therebetween, technology is loaded down with trivial details, and can't carry out fermentation operation continuously; This technology fermentation time is long, and alcohol output is low, does not meet industrial high-yield and high-efficiency requirement.The name of the applicant's application is called the patent (publication number: used gas-lift fermentation reactor CN1403579) of " adopting the airlift fermentation separation coupling technology to prepare the method and the specific equipment of alcohol ", two active carbon adsorption columns with parallel connection obtain alcohol product after the alcohol gas condensation; But this equipment can't be realized the operate continuously of solid state fermentation, can only carry out batch fermentation, for a large amount of fermenting substrates, needs fermentation several times just can finish, and relatively wastes time and energy.
Summary of the invention
The objective of the invention is the method and apparatus that utilizes the sweet sorghum solid-state fermentation and preparation of alcohol at existing, the defective that can't produce continuously; A kind of equipment that utilizes the sweet sorghum stalk solid-state fermentation and preparation of alcohol is provided, this equipment will ferment to carry, adsorb with gas reclaiming and combine, beginning to no longer needing the alcohol whole process obtaining artificial material to shift from fermentation, is the equipment that can realize the preparation Alcohol Production technology that settles at one go.
Another object of the present invention provides a kind of method of utilizing the sweet sorghum stalk solid-state fermentation and preparation of alcohol, and this method will be fermented to separate and is coupled, and reduce operation, reduce production cost, increase profit, improve economic benefit.
The object of the present invention is achieved like this:
The equipment that utilizes the sweet sorghum stalk solid-state fermentation and preparation of alcohol provided by the invention comprises: fermentor tank 2, activated carbon adsorption post 4,5, receptor 7, gas-holder 8 and CO
2Gas cylinder 9; It is characterized in that, comprise also that gas carries jars 3, condenser 6, stepper-motor 10, ethanol concn survey meter 11,12 and pneumatic pump 14; In the wherein said fermentor tank 2 spiral propeller is set, upper housing is provided with feeding unit air sealer 1, and these fermentor tank 2 accumbency and described gas are proposed jar 3 vertical installations and is communicated with and is integral; Described stepper-motor 10 is electrically connected with the spiral propeller of fermentor tank inside, and when starting stepper-motor, spiral propeller can promote material and advance; Described activated carbon adsorption post has 2 at least, and these 2 activated carbon adsorption post outsides are equipped with electric heater unit; The outside of described fermentor tank 2 and described gas tank 3 is equipped with water-cooling jacket, and by temperature in the recirculated water regulating tank, described gas is put forward jar 3 lower port and passed through CO
2Recycle gas line and CO
2Gas cylinder 9 is communicated with described CO
2Recycle gas line also is connected with compressor 22, and this compressor 22 is communicated with gas-holder 8 by pipeline 20; Described gas carry jars 3 upper port by pipeline respectively with the first activated carbon adsorption post 4, the lower port of the second activated carbon adsorption post 5 connects, this pipeline is provided with and is used for detecting the first ethanol concn survey meter 11 that whether also has alcohol to use from gas is carried the gas that comes out the jar, two activated carbon adsorption posts are installed in parallel, first valve 16 that switches usefulness is installed in two activated carbon adsorption post lower port parallel pipelines, and in two activated carbon adsorption post upper port parallel pipelines, install to switch second valve 18 of usefulness, can be by first valve 16 and 18 actings in conjunction of second valve in the parallel pipeline, and carry out the switching of activated carbon adsorption post, the described first activated carbon adsorption post 4, the second activated carbon adsorption post 5 is connected to described gas-holder 8 by pipeline 19, and this gas-holder 8 is by gas circuit 20 and CO
2Gas cylinder 9 connects; Through CO behind the adsorption column
2Carrier gas is come out from the adsorption column upper end, gets back to gas-holder 8 by gas circuit, after compression, arrives CO
2Gas cylinder 9 is realized CO
2Be recycled, so just formed a loop; The described first activated carbon adsorption post 4, the described second activated carbon adsorption post, 5 lower port are received pneumatic pump 14 mutually with airline respectively; Two activated carbon adsorption post upper port link to each other with described condenser 6 by gas piping 21, and this condenser 6 is communicated with receptor 7, also connects the second ethanol concn survey meter 12 on the gas piping 21.
When wherein adsorption column absorption alcohol reach saturated after, utilize the electric heater unit of adsorption column outside to be heated to 90 ℃, under the effect of the air that enters by pneumatic pump 14 compressions, take alcohol in condenser condensation, condensed alcohol receives with receptor 7.
In above-mentioned technical scheme, the end of described fermentor tank 2 is carried jar with gas and was connected as one 3 vertical connections by flange, connects by flange and is convenient to disassembly, cleaning.
In above-mentioned technical scheme, also be included in CO
2One mass flow controller 13 is set on the recycle gas line, should regulates CO by mass flow controller 13
2Flow.
In above-mentioned technical scheme, also be included in the described first activated carbon adsorption post 4, the described second activated carbon adsorption post, 5 lower port and the airline that pneumatic pump 14 is connected one first T-valve 15 is set.
In above-mentioned technical scheme, also be included in one second T-valve 17 is installed in the described pipeline 19.
The equipment of the preparation alcohol that application provided by the invention is above-mentioned carries out the method for fermentative preparation alcohol, comprises the steps:
(1) raw material is prepared: pulverize after the sweet sorghum stalk after the maturation results is removed Chinese sorghum fringe, Chinese sorghum leaf and root; With the sweet sorghum stalk pH value after acid or the alkali adjustment pulverizing is 2.5-7.0;
(2) sterilization: the sweet sorghum stalk after step (1) pulverizing is put into Autoclave sterilize, sterilising temp is at 121-125 ℃, and sterilization time is 15-20min;
(3) activated yeast inoculation: active dry yeast is mixed with sterilized water, blending ratio is pressed 1g: 20mL-1g: 40mL (1g yeast, 20-40mL sterilized water) activates under activation temperature 32-35 ℃, soak time is 25-30min, places 45-60min under the room temperature after the activation again; In the aseptic processing room, add the active dry yeast of stalk weight 0.2-10% again;
(4) fermentation: with the sweet sorghum fermentation substrate behind step (3) disinfection inoculation, join in the fermentor tank according to the input speed of 10L/30min, and the sealing and fermenting jar ferments, leavening temperature remains on 34 ℃-36 ℃, and the 24h secondary fermentation finishes;
(5) gas is carried: when carrying out step (4) fermentation, the sweet sorghum substrate of fermentation is advanced according to predetermined fltting speed, arrive gas behind the 24h just and carry in jars 3, utilize gas to carry the CO that jar 3 belows enter
2Recycle gas is taken away the alcohol that fermentation obtains, wherein CO from the sweet sorghum substrate
2The flow velocity of recycle gas is 2-10L/min;
(6) absorption: carry jar 3 effusive CO from gas
2With the mixed gas of alcohol, be adsorbed remaining CO through two activated carbon adsorption post alcohol in parallel
2Can be recycled after the gas compression;
(7) desorb: with ethanol concn survey meter 11 detect do not have alcohol in the gas mixture after, the activated carbon adsorption column temperature is elevated to 90 ℃, the alcohol that makes absorption desorb from the activated carbon adsorption post is got off;
(8) condensation is reclaimed: utilize the airflow that enters from activated carbon adsorption post lower end, the alcohol that will get off from desorb on the activated carbon adsorption post is brought condenser 6 into, reclaims after the condensation, just obtains alcohol.
Also comprise postprocessing working procedures, the sweet sorghum residue after the fermentation is carried a jar lower end from gas release, as paper making raw material and microbiological fermented feed.
In above-mentioned technical scheme, comprise that also interpolation can provide essential nutrient for fermentation, can participate in constituting the thalline composition, as the integral part of enzyme or keep the activity of enzyme, and regulate the nutritive salt of osmotic pressure; This nutritive salt MgSO wherein
4, KH
2PO
4, CaCl
2Microbial growth and fermentation are had the greatest impact, and nitrogenous source is by (NH4)
2SO
4Come supply; (NH4)
2SO
4Increment press the sweet sorghum stalk weight ratio and add 0.25%, 0.5%, 1.0% (NH4)
2SO
4CaCl wherein
2Addition is pressed sweet sorghum stalk weight ratio adding 0%, 0.5%, 1.0%; MgSO wherein
4Addition is pressed the sweet sorghum stalk weight ratio and is added 0%, 0.5%, 1.0%; KH wherein
2PO
4Addition is pressed the sweet sorghum stalk weight ratio and is added 0%, 0.5%, 1.0%, according to L
9(3
4) carry out orthogonal experiment;
In above-mentioned technical scheme, also comprise when activated carbon adsorption post absorption alcohol reach saturated after, utilize the electric heater unit of activated carbon adsorption post outside to heat, being heated to temperature is 90-100 ℃.
In above-mentioned technical scheme, described acid is the HCl of 0.1-1.0mol/L, or described alkali is the NaOH of 0.1-1.0mol/L.
In above-mentioned technical scheme, the gentle temperature control of carrying jar of fermentor tank, by regulating the gentle circulating water temperature of carrying in jar outside recirculated water chuck of fermentor tank, the temperature in the maintenance fermentor tank is 35 ℃, gas is put forward jar inner control at 40 ℃.
Advantage of the present invention:
1. provide a cover continuous solid state fermentation equipment, comprised five parts: fermentation, gas carries, absorption, condensation, recovery, finishes the process of product alcohol from fermenting to obtaining jointly; Particularly fermentor tank is carried jar vertical being connected with gas, being sealed into one makes the material of fermentation finished thoroughly1 directly fall to arriving the alcoholic extraction stage, do not need artificial material to shift, product alcohol is all finished in this equipment, avoid the influence of external environment, saved time and manpower; And this equipment is applicable to that all granular fermentation material carry out the experiment of solid state fermentation alcohol.
2. utilize continuous solid-state fermentation equipment of the present invention, carry out the mode of direct producing alcohol by solid-state fermentation, this equipment will ferment and be coupled with separating, and utilize CO
2As the circulation carrier gas method that the alcohol that produces separates from the fermentation material of will fermenting, can reduce the influence of the heat that produces in the fermenting process on the one hand to fermentation, can alleviate the restraining effect of alcohol on the other hand.
3. method provided by the invention can be fermented according to the test optimal conditions and be got final product, and does not need to squeeze the juice, and has reduced the operation of producing, and the save operation expense.
4. continuous solid-state fermentation method of the present invention is carried out in a sealing equipment, has solved the pollution problem that exists in the liquid fermenting, will ferment to separate to be coupled, and has reduced operation equally, has reduced production cost, has increased profit, has improved economic benefit.
5. continuous solid-state fermentation method of the present invention also is included in the sweet sorghum substrate and adds and can participate in formation thalline composition for fermentation provides essential nutrient, as the integral part of enzyme or keep the activity of enzyme, and the nutritive salt of adjusting osmotic pressure; This nutritive salt MgSO
4, KH
2PO
4, CaCl
2Microbial growth and fermentation are had the greatest impact, and nitrogenous source is by (NH4)
2SO
4Come supply; Make sweet sorghum solid state fermentation alcohol output improve owing to added nutritive salt, the ethanol production of last gained is 87.82% of a theoretical yield.
6. this method also comprises postprocessing working procedures, the sweet sorghum residue after the fermentation is carried a jar lower end from gas release, as paper making raw material and microbiological fermented feed.
Description of drawings
Fig. 1 is that equipment of the present invention is formed synoptic diagram
Drawing is described as follows:
1-air sealer 2-fermentor tank 3-gas is carried jar
The 4-first activated carbon adsorption post 5-second activated carbon adsorption post 6-condenser
7-receptor 8-gas-holder 9-CO
2Gas cylinder
The 10-stepper-motor 11-first ethanol concn survey meter 12-second ethanol concn survey meter
13-mass flow controller 14-pneumatic pump 15-first T-valve
The 16-first valve 17-second T-valve 18-second valve
19-pipeline 20-CO
2Recycle gas line 21-gas piping
The 22-compression pump
Embodiment
Below in conjunction with drawings and Examples the present invention is described in detail
With reference to figure 1, make an equipment that utilizes the sweet sorghum stalk solid-state fermentation and preparation of alcohol of the present invention.With stainless steel processing and fabricating one volume is fermentor tank 2 (the partly long 1225mm of being of fermentor tank of 15L, internal diameter is 125mm), in this jar spiral propeller is set, these fermentor tank 2 upper housings are provided with a feeding unit air sealer 1, these fermentor tank 2 accumbency settings, cylindraceous, the cylinder bottom that it and one make with stainless steel is that the gas of taper is carried jar 3 vertical connections and is mounted to one, for example connects as one by flange, connects by flange and is convenient to disassembly, cleaning.Promote spiral propeller progressive stepper-motor 10 and be electrically connected with the spiral propeller of fermentor tank inside, when starting stepper-motor, spiral propeller can promote material and advance, and what the time of advancing of material can pre-set stepper-motor advances the time; The activated carbon adsorption post has 2 in the present embodiment, is installed in parallel; These 2 activated carbon adsorption post outsides are equipped with electric heater unit, can utilize the electric heater unit of activated carbon adsorption post outside to heat, being heated to temperature is 90 ℃-100 ℃, compress under the effect of the air that enters by pneumatic pump again, take alcohol in condenser condensation, condensed alcohol receives with receptor.The outside of fermentor tank 2 and gas tank 3 is equipped with water-cooling jacket, by temperature in the recirculated water regulating tank.Gas is put forward jar 3 lower port and is passed through CO
2Recycle gas line and CO
2Gas cylinder 9 is communicated with CO
2Recycle gas line also is connected with compressor 22, and this compressor 22 is communicated with gas-holder 8 by pipeline 20; The upper port that gas is carried jar 3 is connected with the lower port of the first activated carbon adsorption post 4, the second activated carbon adsorption post 5 respectively by pipeline, this pipeline is provided with the first ethanol concn survey meter 11, be used for detecting from gas is carried the gas that comes out the jar and whether also have alcohol, two activated carbon adsorption posts are installed in parallel, alternately adsorb alcohol, after an absorption wherein is saturated, can switch to another root by the valve in the parallel pipeline 16; The described first activated carbon adsorption post 4, the described second activated carbon adsorption post 5 are connected to described gas-holder 8 by pipeline 19, and this gas-holder 8 is by gas circuit 20 and CO
2Gas cylinder 9 connects; Through CO behind the adsorption column
2Carrier gas is come out from the adsorption column upper end, gets back to gas-holder 8 by gas circuit, after compression, arrives CO
2Gas cylinder 9 is realized CO
2Be recycled, so just formed a loop; The described first activated carbon adsorption post 4, the described second activated carbon adsorption post, 5 lower port are received pneumatic pump 14 mutually with airline respectively, two activated carbon adsorption post upper port are by connecting the second ethanol concn survey meter 12 on the gas piping 21, this gas piping 21 links to each other with described condenser 6 again, this condenser 6 is communicated with receptor 7, when wherein adsorption column absorption alcohol reach saturated after, utilize the electric heater unit of adsorption column outside to be heated to 90 ℃, under the effect of the air that enters by pneumatic pump 14 compressions, take alcohol in condenser condensation, condensed alcohol receives with receptor 7.
In the present embodiment in order to regulate CO
2Flow also is included in CO
2One mass flow controller 13 is set on the recycle gas line, reaches by mass flow controller 13 and regulate CO
2The purpose of flow.
First T-valve 15 also is set in airline in the present embodiment, and second T-valve 17 also is installed in pipeline 19.
In the equipment of embodiment 1, carry out the method for sweet sorghum stalk solid-state fermentation and preparation of alcohol, comprise the steps:
(1) raw material is prepared: the sweet sorghum stalk after the maturation results is removed sweet sorghum stalk behind Chinese sorghum fringe, Chinese sorghum leaf and the root, and it is standby to be crushed to 0.5cm; Adjust the initial pH value to 2.5 of sweet sorghum stalk material with the HCl of 0.1mol/L;
In addition, can also be added to fermentation essential nutrient is provided, can participate in formation thalline composition, as the integral part of enzyme or keep the activity of enzyme, and the nutritive salt of adjusting osmotic pressure, so can also add for example MgSO of nutritive salt in an embodiment
4, KH
2PO
4Or CaCl
2, they have the greatest impact to microbial growth and fermentation, also can supply nitrogen salt, for example (NH4)
2SO
4, increment is pressed (NH4) of sweet sorghum stalk weight ratio adding 0.25%, 0.5%, 1.0%
2SO
4CaCl wherein
2Addition is pressed sweet sorghum stalk weight ratio adding 0%, 0.5%, 1.0%; MgSO wherein
4Addition is pressed the sweet sorghum stalk weight ratio and is added 0%, 0.5%, 1.0%; KH wherein
2PO
4Addition is pressed the sweet sorghum stalk weight ratio and is added 0%, 0.5%, 1.0%, according to L
9(3
4) carry out orthogonal experiment; And add 0.5% (NH4)
2SO
4With the CaCl that adds 0.5%
2Effect is best, and the ethanol production of last gained is 87.82% of a theoretical yield.
(2) sterilization: the sweet sorghum stalk after step (1) pulverizing is put into Autoclave, under 121 ℃ of temperature, take out behind the 20min that sterilizes;
(3) activated yeast inoculation: high temperature resistant wine brewing active dry yeast is mixed with 20mL distilled water by the 1g yeast with sterilized water, activate 30min down, place 1h under the room temperature again at 35 ℃; On the indoor super clean bench of aseptic technique, inoculate, add the active dry yeast of stalk weight 0.2% again, after inoculation finishes, join in the solid state fermentation equipment;
(4) fermentation: with the sweet sorghum fermentation substrate behind step (3) disinfection inoculation, join in the fermentor tank according to the input speed of predetermined 10L/30min, and the sealing and fermenting jar ferments, leavening temperature remains on 35 ℃, and the 24h secondary fermentation finishes; Embodiment adds thermal recovery to the recirculated water heating in the recirculated water chuck of fermentor tank outside, and by the recirculated water attemperation, the temperature of fermentation part finishes after remaining on the 24h that ferments under 35 ℃ (precision is for ± 1 ℃);
(5) gas is carried: when carrying out step (4) fermentation, the sweet sorghum substrate that ferments is advanced according to predetermined fltting speed, arrive gas behind the 24h just and carry in jar (3), utilize gas to carry the CO that jar (3) below enters
2Recycle gas, this CO
2The flow velocity of recycle gas is 2-10L/min, and the alcohol that fermentation obtains is taken away from the sweet sorghum substrate; Present embodiment is carried a jar outside recirculated water jacket temperature by regulating gas, and gas is put forward part and is controlled at 40 ℃, (temperature accuracy is ± 1 ℃);
(6) absorption: CO
2And the mixed gas of alcohol, through two in parallel activated carbon adsorption posts (two adsorption column parallel connections after the alcohol that records an adsorption column with ethanol concn survey meter 11 reaches capacity, can switch to another adsorption column), alcohol in the gas mixture is adsorbed, remaining CO
2Can be recycled after the gas compression;
(7) desorb: with ethanol concn survey meter 11 detect do not have alcohol in the gas mixture after, the activated carbon adsorption column temperature is elevated to 90 ℃, the alcohol that makes absorption desorb from the activated carbon adsorption post is got off.
(8) condensation is reclaimed: utilize the airflow that enters from activated carbon adsorption post lower end, the alcohol that will get off from desorb on the activated carbon adsorption post is brought condenser 6 into, reclaims after the condensation, just obtains alcohol.
In the equipment of embodiment 1, carry out the method for sweet sorghum stalk solid-state fermentation and preparation of alcohol, comprise the steps:
(1) raw material is prepared: the sweet sorghum stalk after maturation is gathered in the crops removes the sweet sorghum stalk behind Chinese sorghum fringe, Chinese sorghum leaf and the root, is crushed to 0.5cm, and is stand-by; HCl with 0.1mol/L adjusts the initial pH value to 5.0 that sweet sorghum is expected, presses weight of material than (NH4) that add 0.5%
2SO
4, 0.5% CaCl
2, 1.0% MgSO
4Other preparation process repeats the step of embodiment 2.
Embodiment 4
In the equipment of embodiment 1, carry out the method for solid-state fermentation and preparation of alcohol, comprise the steps:
(1) raw material is prepared: the sweet sorghum stalk after maturation is gathered in the crops removes the sweet sorghum stalk behind Chinese sorghum fringe, Chinese sorghum leaf and the root, is crushed to 0.5cm, and is stand-by; NaOH with 0.1mol/L adjusts the initial pH value to 7.0 that sweet sorghum is expected, presses weight of material than (NH4) that add 1.0%
2SO
4, 0.5% CaCl
2, 1.0% KH
2PO
4Other preparation process repeats the step of embodiment 2.
Embodiment 5
Use embodiment 1 equipment to carry out the method for solid-state fermentation and preparation of alcohol, comprise the steps:
(1) raw material is prepared: the sweet sorghum stalk after maturation is gathered in the crops removes the sweet sorghum stalk behind Chinese sorghum fringe, Chinese sorghum leaf and the root, is crushed to 0.5cm, and is stand-by; HCl with 0.1mol/L adjusts the initial pH value to 5.0 that sweet sorghum is expected, presses weight of material than (NH4) that add 0.5%
2SO
4, 0.5% CaCl
2Other preparation process repeats the step of embodiment 2.
Claims (9)
1. an equipment that utilizes the sweet sorghum stalk solid-state fermentation and preparation of alcohol comprises: fermentor tank (2), activated carbon adsorption post, receptor (7), gas-holder (8) and CO
2Gas cylinder (9); It is characterized in that, comprise that also gas carries jar (3), a condenser (6), stepper-motor (10), ethanol concn survey meter (12) and pneumatic pump (14); Wherein said fermentor tank is provided with spiral propeller in (2), upper housing is provided with feeding unit air sealer (1), (2) accumbency of this fermentor tank and described gas are carried jar (a 3) vertical connection and are mounted to one, and the outside that described fermentor tank (2) and described gas are carried jar (3) is equipped with water-cooling jacket; Described stepper-motor (10) is electrically connected with the spiral propeller of fermentor tank inside; Described activated carbon adsorption post has 2 at least, and these 2 activated carbon adsorption post outsides are equipped with electric heater unit; Described gas is put forward jar (a 3) lower port and is passed through CO
2Recycle gas line (20) and CO
2Gas cylinder (9) is communicated with, and also order is communicated with compressor (22) and gas-holder (8); The upper port that described gas is carried jar (3) by pipeline respectively with the first activated carbon adsorption post (4), the bottom of the second activated carbon adsorption post (5) connects, this pipeline is provided with the first ethanol concn survey meter (11), two activated carbon adsorption posts are installed in parallel, at the first activated carbon adsorption post (4), first valve (16) that switches usefulness is installed in second activated carbon adsorption post (5) the lower port parallel pipeline, and at the first activated carbon adsorption post (4), second valve (18) that switches usefulness is installed in second activated carbon adsorption post (5) the upper port parallel pipeline, this second valve (18) is connected with gas piping (21), described gas piping (21) order and the second ethanol concn survey meter (12), condenser (6) and receptor (7) are communicated with; The top of the described first activated carbon adsorption post (4), the second activated carbon adsorption post (5) connects a pipe respectively and is connected with pipeline (19) by second T-valve (17), and the end of this pipeline (19) is connected to described gas-holder (8); The described first activated carbon adsorption post (4), described second activated carbon adsorption post (5) lower port are received pneumatic pump (14) mutually with airline respectively.
2. by the described equipment that utilizes the sweet sorghum stalk solid-state fermentation and preparation of alcohol of claim 1, it is characterized in that, also be included in CO
2One mass flow controller (13) is set on the recycle gas line.
3. by the described equipment that utilizes the sweet sorghum stalk solid-state fermentation and preparation of alcohol of claim 1, it is characterized in that, also be included in the described first activated carbon adsorption post (4), the described second activated carbon adsorption post (5), in lower port and the airline that pneumatic pump (14) is connected one first T-valve (15) be set.
4. by claim 1, the 2 or 3 described equipment that utilize the sweet sorghum stalk solid-state fermentation and preparation of alcohol, it is characterized in that an end of described fermentor tank (2) is carried jar with gas and connected as one (3) vertical connection by flange.
5. an application rights requires the 1 described equipment that utilizes the sweet sorghum stalk solid-state fermentation and preparation of alcohol to carry out the method for fermentative preparation alcohol, comprises the steps:
(1) raw material is prepared: pulverize after the sweet sorghum stalk after the maturation results is removed Chinese sorghum fringe, Chinese sorghum leaf and root; With the sweet sorghum stalk pH value after acid or the alkali adjustment pulverizing is 2.5-7.0;
(2) sterilization: the sweet sorghum stalk after step (1) pulverizing is put into Autoclave sterilize, sterilising temp is at 121-125 ℃, and sterilization time is 15-20min;
(3) activated yeast inoculation: active dry yeast is mixed with sterilized water, blending ratio is pressed yeast 1g: sterilized water 20mL-yeast 1g: sterilized water 40mL, activate under activation temperature 32-35 ℃, soak time is 25-30min, places 45-60min under the room temperature after the activation again; In the aseptic processing room, add the active dry yeast of stalk weight 0.2-10% again;
(4) fermentation: with the sweet sorghum fermentation substrate behind step (3) disinfection inoculation, join in the fermentor tank according to predetermined input speed, and the sealing and fermenting jar ferments, leavening temperature remains on 35 ℃, and the 24h secondary fermentation finishes;
(5) gas is carried: when carrying out step (4) fermentation, the sweet sorghum substrate that ferments is advanced according to predetermined fltting speed, arrive gas behind the 24h just and carry in jar (3), utilize gas to carry the CO that jar (3) below enters
2Recycle gas is taken away the alcohol that fermentation produces, wherein CO from the sweet sorghum substrate
2The flow velocity of recycle gas is 2-10L/min;
(6) absorption: carry jar (a 3) effusive CO from gas
2With the mixed gas of alcohol, be adsorbed remaining CO through two activated carbon adsorption post alcohol in parallel
2Can be recycled after the gas compression;
(7) desorb: with ethanol concn survey meter (11) detect do not have alcohol in the gas mixture after, the activated carbon adsorption column temperature is elevated to 90 ℃, the alcohol that makes absorption desorb from the activated carbon adsorption post is got off;
(8) condensation is reclaimed: utilize the airflow that enters from activated carbon adsorption post lower end, the alcohol that will get off from desorb on the activated carbon adsorption post is brought condenser (6) into, reclaims after the condensation, just obtains alcohol product.
6. by the described method of utilizing the sweet sorghum stalk solid-state fermentation and preparation of alcohol of claim 5, it is characterized in that, also comprise and add MgSO
4, KH
2PO
4, CaCl
2Or (NH
4)
2SO
4Nutritive salt; Wherein (NH4)
2SO
4Add-on is 0.25%-1.0% by the sweet sorghum stalk weight ratio; CaCl wherein
2Add-on is 0.5%-1.0% by the sweet sorghum stalk weight ratio; MgSO wherein
4Add-on is 0.5%-1.0% by the sweet sorghum stalk weight ratio; Or KH wherein
2PO
4Add-on is 0%-1.0% by the sweet sorghum stalk weight ratio.
7. by the described method of utilizing the sweet sorghum stalk solid-state fermentation and preparation of alcohol of claim 5, it is characterized in that, also comprise and add MgSO
4And CaCl
2,MgSO wherein
4Add-on is 0.25%-1.0%, wherein CaCl by the sweet sorghum stalk weight ratio
2Add-on is 0.5%-1.0% by the sweet sorghum stalk weight ratio.
8. by the described method of utilizing the sweet sorghum stalk solid-state fermentation and preparation of alcohol of claim 5, it is characterized in that described acid is the HCl of 0.1-1.0mol/L, or described alkali is the NaOH of 0.1-1.0mol/L.
9. by the described method of utilizing the sweet sorghum stalk solid-state fermentation and preparation of alcohol of claim 5, it is characterized in that, the gentle temperature control of carrying jar of described fermentor tank, by regulating the gentle circulating water temperature of carrying in jar outside recirculated water chuck of fermentor tank, keeping the temperature in the fermentor tank is 35 ℃, and gas is put forward jar inner control at 40 ℃.
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| CN200610112613A CN101130791B (en) | 2006-08-25 | 2006-08-25 | Device and method for solid-state fermentation and preparation of alcohol using sorgo stalk |
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| CN101130791B true CN101130791B (en) | 2010-05-12 |
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Cited By (1)
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| EP2698423A2 (en) * | 2011-04-14 | 2014-02-19 | GS Caltex Corporation | Apparatus and method for separating and refining fermentation of product manufactured by fermenting microorganism by using adsorbent |
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| CN101768539B (en) * | 2010-02-24 | 2012-11-14 | 中国科学院过程工程研究所 | Reactor for preparing ethanol and butanol by utilizing sorgo stalk solid anaerobic digestion |
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| CN103509712B (en) * | 2013-08-07 | 2015-11-18 | 清华大学 | A kind of device and technique possessing the continuous solid-state fermentation ethanol production of robot control system(RCS) |
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