CN114344335A - Medicine for treating erectile dysfunction and preparation method thereof - Google Patents
Medicine for treating erectile dysfunction and preparation method thereof Download PDFInfo
- Publication number
- CN114344335A CN114344335A CN202210153771.9A CN202210153771A CN114344335A CN 114344335 A CN114344335 A CN 114344335A CN 202210153771 A CN202210153771 A CN 202210153771A CN 114344335 A CN114344335 A CN 114344335A
- Authority
- CN
- China
- Prior art keywords
- layer
- erectile dysfunction
- treating erectile
- medicine
- cgf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 201000001881 impotence Diseases 0.000 title claims abstract description 49
- 208000010228 Erectile Dysfunction Diseases 0.000 title claims abstract description 48
- 239000003814 drug Substances 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 229940079593 drug Drugs 0.000 title claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 21
- 210000005259 peripheral blood Anatomy 0.000 claims abstract description 14
- 239000011886 peripheral blood Substances 0.000 claims abstract description 14
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 9
- 239000003292 glue Substances 0.000 claims abstract description 9
- 238000000926 separation method Methods 0.000 claims abstract description 9
- 238000001085 differential centrifugation Methods 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 10
- 238000001816 cooling Methods 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 7
- 210000004369 blood Anatomy 0.000 claims description 3
- 239000008280 blood Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 claims 4
- 238000004519 manufacturing process Methods 0.000 claims 1
- 210000003899 penis Anatomy 0.000 abstract description 6
- 206010036596 premature ejaculation Diseases 0.000 abstract description 5
- 230000036299 sexual function Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 208000024891 symptom Diseases 0.000 abstract description 2
- 210000001808 exosome Anatomy 0.000 description 5
- 230000001225 therapeutic effect Effects 0.000 description 4
- 239000002571 phosphodiesterase inhibitor Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 229940099471 Phosphodiesterase inhibitor Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940082638 cardiac stimulant phosphodiesterase inhibitors Drugs 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000008451 emotion Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000009323 psychological health Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/10—Drugs for genital or sexual disorders; Contraceptives for impotence
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Reproductive Health (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Gynecology & Obstetrics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hematology (AREA)
- Endocrinology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Zoology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention belongs to the technical field of medicines, and discloses a medicine for treating erectile dysfunction and a preparation method thereof. The invention selects the peripheral blood of the centrifuge to carry out differential centrifugation to obtain the layering from top to bottom, specifically a PPP layer, a CGF layer, a separation glue layer and a red blood cell layer; and preparing the liquid in the PPP layer into PAG gel, and mixing the PAG gel with the liquid in the CGF layer according to the volume ratio of 1-5: 1 to obtain the medicament for treating erectile dysfunction. The medicine prepared by the invention can realize the repair and filling of the cavernous body after being injected into the cavernous body, and treat the symptoms of erectile dysfunction. Can be used for treating short penis and weak sexual function, and has the effects of improving sexual function, delaying time, improving hardness, and preventing sexual impotence and premature ejaculation.
Description
Technical Field
The invention relates to the technical field of medicines, in particular to a medicine for treating erectile dysfunction and a preparation method thereof.
Background
Erectile Dysfunction (ED) is a common, frequently occurring disease in adult males. According to survey, the incidence rate of ED reaches 50% in men between 40 and 70 years, the number of ED patients in China is large, ED does not endanger life, but affects physical and mental health of men, and affects couple emotion, family harmony and social stability.
The causes of ED are numerous, and among them, damaged cavernous body is also the important cause of ED, and the therapeutic effect of the existing therapeutic means is not ideal, for example, phosphodiesterase inhibitors can relieve ED symptoms, but the therapeutic effect lasts for a short time and is expensive, and the therapeutic effect on damaged cavernous body is not obvious.
Besides the erectile dysfunction, most men have the problems of short penis and premature ejaculation, and the physical and psychological health is seriously affected. At the present stage, effective medicines for simultaneously treating erectile dysfunction, short penis, premature ejaculation and other problems are rarely reported.
Therefore, how to provide a medicament for treating erectile dysfunction and a preparation method thereof, which can relieve the problems of erectile dysfunction, short penis, premature ejaculation and the like, and reduce the cost of the medicament is a difficult problem to be solved urgently in the field.
Disclosure of Invention
In view of the above, the invention provides a medicament for treating erectile dysfunction and a preparation method thereof, the raw materials used by the invention are cheap and easy to obtain, the preparation method is simple, the problem of high cost of the medicament for treating erectile dysfunction is solved, and the medicament effect is improved.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of a medicine for treating erectile dysfunction comprises the following steps:
1) centrifuging the peripheral blood of the excised body, and dividing the blood into a PPP layer, a CGF layer, a separation glue layer and a red blood cell layer from top to bottom;
2) preparing PAG gel from the liquid in the PPP layer obtained in the step 1);
3) mixing PAG gel with the liquid in CGF layer to obtain the medicine for treating erectile dysfunction.
Preferably, the centrifugation in the step 1) is differential centrifugation, and the differential centrifugation condition is 1500-2000 rpm centrifugation for 7-10 min.
Preferably, the PAG gel is prepared as follows:
heating the PPP layer obtained in the step 1) at 70-80 ℃ for 3-10 min, and cooling to obtain PAG gel.
Preferably, the volume ratio of the PAG gel to the liquid in the CGF layer in the step 3) is 1-5: 1.
preferably, the mixing operation in the step 3) is stirring, the stirring speed is 80-200 rpm, and the stirring time is 1-3 min.
The invention also aims to provide the drug for treating erectile dysfunction, which is prepared by the preparation method of the drug for treating erectile dysfunction.
Through the technical scheme, compared with the prior art, the invention has the following beneficial effects:
the medicine for treating erectile dysfunction prepared by the invention is injected into the cavernous body, so that the repair and filling of the cavernous body can be realized, and the cavernous body repair and the erectile dysfunction treatment can be carried out on patients with damaged cavernous body. The penis health care product has the effects of enhancing sexual function, delaying time, improving hardness and preventing impotence and premature ejaculation for people with short and small penis and weak sexual function;
the medicine for treating erectile dysfunction disclosed by the invention is simple in preparation method, low in cost and convenient to popularize and apply on a large scale, and raw materials are cheap and easily available.
Detailed Description
A preparation method of a medicine for treating erectile dysfunction comprises the following steps:
1) centrifuging the peripheral blood of the excised body, and dividing the blood into a PPP layer, a CGF layer, a separation glue layer and a red blood cell layer from top to bottom;
2) preparing PAG gel from the liquid in the PPP layer obtained in the step 1);
3) mixing PAG gel with the liquid in CGF layer to obtain the medicine for treating erectile dysfunction.
In the invention, the centrifugation in the step 1) is differential centrifugation, the differential centrifugation condition is 1500-2000 rpm centrifugation for 7-10 min, and the centrifugation time is preferably 8 min.
In the present invention, the PAG gel is prepared as follows:
heating the PPP layer obtained in the step 1) at 70-80 ℃ for 3-10 min, and cooling to obtain PAG gel, wherein the heating temperature is preferably 74-78 ℃, and further preferably 75 ℃; the heating time is 5-8 min, preferably 7 min.
In the invention, the volume ratio of the PAG gel to the liquid in the CGF layer in the step 3) is 1-5: 1, preferably 2-4: 1, more preferably 3: 1.
in the invention, the mixing operation in the step 3) is stirring, and the stirring speed is 80-200 rpm, preferably 90-150 rpm, and more preferably 110 rpm; the stirring time is 1-3 min, preferably 2 min.
The invention also provides a medicine for treating erectile dysfunction, which is prepared by the preparation method of the medicine for treating erectile dysfunction.
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Taking the peripheral blood of the exosome, centrifuging for 8min at a differential speed under the condition of 1500-2000 rpm, and dividing the peripheral blood into a PPP layer, a CGF layer, a separation glue layer and a red blood cell layer from top to bottom; heating the liquid in the PPP layer at 75 deg.C for 7min, and cooling to obtain PAG gel; PAG gel was mixed with liquid in CGF layer at 3: 1 (stirring at 110rpm for 2min) to obtain the medicine for treating erectile dysfunction.
Example 2
Taking the peripheral blood of the exosome, centrifuging for 9min at a differential speed of 1500-2000 rpm, and dividing the peripheral blood into a PPP layer, a CGF layer, a separation glue layer and a red blood cell layer from top to bottom; heating the liquid in the PPP layer at 74 deg.C for 7min, and cooling to obtain PAG gel; PAG gel was mixed with liquid in CGF layer at 3: 1 (stirring at 90rpm for 2min) to obtain the medicine for treating erectile dysfunction.
Example 3
Taking the peripheral blood of the exosome, centrifuging for 7min at a differential speed under the condition of 1500-2000 rpm, and dividing the peripheral blood into a PPP layer, a CGF layer, a separation glue layer and a red blood cell layer from top to bottom; heating the liquid in the PPP layer at 70 deg.C for 10min, and cooling to obtain PAG gel; PAG gel was mixed with liquid in CGF layer at 1: 1 (stirring at 110rpm for 3min) to obtain the medicine for treating erectile dysfunction.
Example 4
Taking the peripheral blood of the exosome, centrifuging for 8min at a differential speed under the condition of 1500-2000 rpm, and dividing the peripheral blood into a PPP layer, a CGF layer, a separation glue layer and a red blood cell layer from top to bottom; heating the liquid in the PPP layer at 73 deg.C for 5min, and cooling to obtain PAG gel; PAG gel was mixed with liquid in CGF layer at 3: 1 (stirring at 140rpm for 2min) to obtain the medicine for treating erectile dysfunction.
Example 5
Taking the peripheral blood of the exosome, centrifuging for 10min at a differential speed under the condition of 1500-2000 rpm, and dividing the peripheral blood into a PPP layer, a CGF layer, a separation glue layer and a red blood cell layer from top to bottom; heating the liquid in the PPP layer at 80 deg.C for 3min, and cooling to obtain PAG gel; the PAG gel was mixed with the liquid in the CGF layer at 5:1 (stirring at 150rpm for 1min) to obtain the medicine for treating erectile dysfunction.
Experimental example 1
60 male erectile dysfunction patients were selected and randomly divided into 6 groups of 10 patients each, the first 6 groups were numbered as experimental groups, and drugs for treating erectile dysfunction prepared in examples 1 to 5 were injected correspondingly, and the 7 th group was a control group, and a commercially available phosphodiesterase inhibitor drug was used.
The patients did not take any other drugs within the following period, and the test results are shown in table 1.
TABLE 1 use of drug State Table
As can be seen from Table 1, the medicament prepared by the present invention has significant effects on various populations and is effective for a long time. In fact, the feedback reaction not only solves the erectile dysfunction, but also can enhance the sexual function.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. The preparation method of the medicine for treating erectile dysfunction is characterized by comprising the following steps:
1) centrifuging the peripheral blood of the excised body, and dividing the blood into a PPP layer, a CGF layer, a separation glue layer and a red blood cell layer from top to bottom;
2) preparing PAG gel from the liquid in the PPP layer obtained in the step 1);
3) mixing PAG gel with the liquid in CGF layer to obtain the medicine for treating erectile dysfunction.
2. The method for preparing a medicament for treating erectile dysfunction according to claim 1, wherein the centrifugation in the step 1) is differential centrifugation, and the differential centrifugation condition is 1500-2000 rpm centrifugation for 7-10 min.
3. The process for preparing a drug for treating erectile dysfunction according to claim 2, wherein the PAG gel is prepared by the following steps:
heating the PPP layer obtained in the step 1) at 70-80 ℃ for 3-10 min, and cooling to obtain PAG gel.
4. The method for preparing a drug for treating erectile dysfunction according to any one of claims 1 to 3, wherein the volume ratio of the PAG gel to the liquid in the CGF layer in the step 3) is 1 to 5: 1.
5. the method for preparing a medicament for treating erectile dysfunction according to claim 4, wherein the mixing operation in the step 3) is stirring, the stirring speed is 80-200 rpm, and the stirring time is 1-3 min.
6. A medicament for treating erectile dysfunction prepared by the process for the preparation of a medicament for treating erectile dysfunction according to any one of claims 1 to 5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210153771.9A CN114344335A (en) | 2022-02-19 | 2022-02-19 | Medicine for treating erectile dysfunction and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210153771.9A CN114344335A (en) | 2022-02-19 | 2022-02-19 | Medicine for treating erectile dysfunction and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114344335A true CN114344335A (en) | 2022-04-15 |
Family
ID=81093926
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210153771.9A Pending CN114344335A (en) | 2022-02-19 | 2022-02-19 | Medicine for treating erectile dysfunction and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114344335A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110721343A (en) * | 2019-11-01 | 2020-01-24 | 河南省直第三人民医院 | GFG gel capable of repairing depressed or atrophic scars, and preparation method and application thereof |
| WO2021009659A1 (en) * | 2019-07-12 | 2021-01-21 | Vasanthi Palanivel | Compositions for treatment of erectile dysfunction, methods for preparing the same and applications thereof |
-
2022
- 2022-02-19 CN CN202210153771.9A patent/CN114344335A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021009659A1 (en) * | 2019-07-12 | 2021-01-21 | Vasanthi Palanivel | Compositions for treatment of erectile dysfunction, methods for preparing the same and applications thereof |
| US20220395558A1 (en) * | 2019-07-12 | 2022-12-15 | Vasanthi PALANIVEL | Compositions for treatment of erectile dysfunction, methods for preparing the same and applications thereof |
| CN110721343A (en) * | 2019-11-01 | 2020-01-24 | 河南省直第三人民医院 | GFG gel capable of repairing depressed or atrophic scars, and preparation method and application thereof |
Non-Patent Citations (9)
| Title |
|---|
| ELŻBIETA CECERSKA-HERYĆ等: "Applications of the regenerative capacity of platelets in modern medicine", CYTOKINE AND GROWTH FACTOR REVIEWS, vol. 64, pages 84 - 94, XP087003141, DOI: 10.1016/j.cytogfr.2021.11.003 * |
| FERNÁNDEZ-PASCUAL, E.等: "Platelet rich plasma for erectile dysfunction: A randomised, double-blind controlled trial to evaluate the efficacy of intracavernosal infusion of Platelet rich plasma (PRP) against platelet poor plasma (PPP) in the treatment of vasculogenic erectile dysfunction", THE JOURNAL OF SEXUAL MEDICINE, vol. 17, pages 136 * |
| JING QIAO等: "Quantification of growth factors in different platelet concentrates", PLATELETS, pages 1369 - 1635 * |
| MING-CHE LIU等: "Revisiting the regenerative therapeutic advances towards erectile dysfunction", CELLS, vol. 09, 19 May 2020 (2020-05-19), pages 1 - 21 * |
| 丁协刚等: "富血小板血浆对大鼠海绵体神经损伤的修复效应", 现代泌尿外科杂志, no. 01, pages 59 - 62 * |
| 姚泽欣等: "浓缩血小板的发展和分类", 医学研究生学报, vol. 35, pages 208 - 213 * |
| 张长青等: "富血小板血浆技术在临床的应用", 31 December 2018, 上海交通大学出版社, pages: 320 * |
| 汪淼等: "CGF在组织再生及整形美容中的基础研究与临床应用进展", 中国美容医学, vol. 29, pages 183 - 186 * |
| 蒋波: "勃起功能障碍与血管内皮因子的相关性研究进展", 现代中西医结合杂志, no. 06, pages 679 - 682 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103520555A (en) | Drug for treatment of migraine | |
| CN114344335A (en) | Medicine for treating erectile dysfunction and preparation method thereof | |
| CN100345566C (en) | Medicine composition used for reducing weight, prepn. method and use thereof | |
| CN108403818B (en) | Composition for assisting in reducing blood sugar and application thereof | |
| CN105362407A (en) | Composition for preventing and/or treating sub-health caused by smoking | |
| CN104324261A (en) | Pharmaceutical composition for treating high blood sugar and preparation method of pharmaceutical composition | |
| CN102380087A (en) | Chinese medicinal composition for treating cough and dyspnea | |
| CN111346211A (en) | Novel medicine with function of treating eye diseases | |
| CN101693086B (en) | Traditional Chinese medicine compound preparation for preventing recurrent aphthae, preparation method and application thereof | |
| CN113694165A (en) | Compound globefish liver oil composition and preparation method and application thereof | |
| US20110200691A1 (en) | Medicine for Filterable Virus Infections and Its Processing Method | |
| CN101468071B (en) | Chinese medicine medicine for suppressing and alleviating sex urge for male and female | |
| CN1730045A (en) | Medicine for treating piles and its preparing process | |
| CN109258869A (en) | A kind of draft solid beverage and preparation method thereof, application method and application | |
| CN102846668A (en) | Preparation method for nanometer Periplaneta americana | |
| CN111714538B (en) | A Chinese medicinal composition for treating hepatitis B infection and preparation method thereof | |
| CN111529658B (en) | Ointment for treating rotavirus enteritis and preparation method thereof | |
| CN111375026B (en) | Traditional Chinese medicine composition for preventing or treating obesity caused by olanzapine and preparation method, pharmaceutical preparation and application thereof | |
| JP2013528188A (en) | Pharmaceutical composition for treating drug addicts | |
| CN107596127B (en) | Traditional Chinese medicine composition for treating hand-foot-and-mouth disease, spray and preparation method | |
| CN115120697A (en) | Tibetan medicine composition and preparation method and application thereof | |
| CN105213575A (en) | A kind of Chinese medicine composition and the application in oncotherapy thereof | |
| CN1357359A (en) | Drug addiction stopping machine medicine and its prepn | |
| CN117899145A (en) | Traditional Chinese medicine composition and medicine for preventing and treating alcoholic liver injury | |
| CN105147769A (en) | Application of rosemary extract to medicine or healthcare product for sleep improvement |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |