CN114343085A - Aquaculture nutritional supplement and preparation method thereof - Google Patents

Aquaculture nutritional supplement and preparation method thereof Download PDF

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Publication number
CN114343085A
CN114343085A CN202210008704.8A CN202210008704A CN114343085A CN 114343085 A CN114343085 A CN 114343085A CN 202210008704 A CN202210008704 A CN 202210008704A CN 114343085 A CN114343085 A CN 114343085A
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China
Prior art keywords
aquaculture
nutritional supplement
powder
parts
chitin
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CN202210008704.8A
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Chinese (zh)
Inventor
李鹏程
范兆乾
秦玉坤
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Institute of Oceanology of CAS
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Institute of Oceanology of CAS
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Priority to CN202210008704.8A priority Critical patent/CN114343085A/en
Publication of CN114343085A publication Critical patent/CN114343085A/en
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Abstract

The invention relates to the field of aquaculture, in particular to an aquaculture nutrition supplement and a preparation method thereof. The nutrition replenisher comprises, by weight, 70-90 parts of shell powder, 8-20 parts of chitin powder, 2-6 parts of artificial zeolite powder, 1-4 parts of alum and 0.3-1.0 part of potassium hydrogen persulfate. The nutritional supplement can provide carbonate concentration and calcium carbonate saturation, and adjust the pH of the water body, thereby avoiding acidification; trace elements, amino acid, carbon source and nitrogen source are supplemented, so that the use of inorganic fertilizer is reduced, and the abundance of microalgae bait is improved; removing hydrogen sulfide in the substrate, reducing the accumulation of nitrite, avoiding water eutrophication, improving the growth environment of the aquaculture animals, finally promoting the growth of the aquaculture animals and improving the yield.

Description

Aquaculture nutritional supplement and preparation method thereof
Technical Field
The invention relates to the field of aquaculture, in particular to an aquaculture nutrition supplement and a preparation method thereof.
Background
Most shellfish live on filter feeding phytoplankton and organic debris. In the shellfish culture process, especially in the seedling stage, microalgae is additionally applied as bait for ensuring the growth of the shellfish, and inorganic fertilizers such as nitrogen, phosphorus, potassium and the like are required as nutrients for culturing the bait. In recent years, the shellfish culture scale development in China is rapid, but the current culture mode is mainly a culture mode of high-density stocking, large amount of fertilization and bait casting. And the inorganic fertilizer is used in a large amount for a long time, so that the problems of water eutrophication, oxygen deficiency, nitrite accumulation and the like are easily caused, the water quality health is influenced, and the ecology of a natural water area is deteriorated. Under the sustainable development environment, the problems of environmental ecology are avoided, the use of inorganic fertilizers is imperative, and the problem of ensuring the growth of baits is a subject to be discussed.
The aquaculture scale of China is huge, and a large amount of leftovers such as shells, spiral shells, shrimp and crab shells and the like can be produced every year. At present, the leftovers are mainly discarded as processing wastes or used for producing low-value feed, and have low resource utilization rate and serious pollution emission. If the leftovers are changed into valuable things and are utilized in a high-value mode, the method has great significance for improving the technical level of sustainable utilization of marine biological resources in China and promoting the sustainable development of marine agriculture and marine biological industry.
Disclosure of Invention
The invention aims to provide an aquaculture nutritional supplement and a preparation method thereof.
In order to achieve the purpose, the invention provides the following technical scheme:
the aquaculture nutrition supplement comprises, by weight, 70-90 parts of shell powder, 8-20 parts of chitin powder, 2-6 parts of artificial zeolite powder, 1-4 parts of alum and 0.3-1.0 part of potassium hydrogen persulfate.
The shell powder is prepared from shell shells or snail shells through cleaning, high-temperature drying, crushing and sieving (100-mesh and 200-mesh); wherein the shellfish shell is one or more of mussel, scallop, clam, oyster and razor clam, and the snail shell is one or more of whelk and conch.
The chitin powder is prepared by crushing chitin with molecular weight of 20-200kDa to particle size of less than 100 meshes; wherein the chitin is chitin and/or its downstream product chitosan.
The artificial zeolite powder is prepared by crushing artificial zeolite to 60-200 meshes for later use.
The nutritional supplement is prepared into powder, granules or pills.
A preparation method of aquaculture nutritional supplement comprises mixing the components at the ratio to obtain aquaculture nutritional supplement.
The use of an aquaculture nutritional supplement for use as an additive to baits in marine aquaculture crop nursery cultures; it is used in an amount of 2-6kg/m2
The aquaculture crops are shellfish, snails, shrimps, crabs and phytophagous fishes; the bait is fresh water or marine microalgae.
The invention has the beneficial effects that:
the nutritional supplement can provide carbonate concentration and calcium carbonate saturation, and adjust the pH of the water body, thereby avoiding acidification; trace elements, amino acid, carbon source and nitrogen source are supplemented, so that the use of inorganic fertilizer is reduced, and the abundance of microalgae bait is improved; removing hydrogen sulfide in the substrate, reducing the accumulation of nitrite, avoiding water eutrophication, improving the growth environment of the aquaculture animals, finally promoting the growth of the aquaculture animals and improving the yield.
Drawings
FIG. 1 is a graph illustrating the effect of nutritional supplements on the pH of a body of water according to embodiments of the present invention.
FIG. 2 is a graph showing the effect of nutritional supplements on photosynthetic pigments of Pachylomyces lanuginosus provided by an embodiment of the present invention.
The specific implementation mode is as follows:
the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The shell is added into the nutritional supplement, so that the nutritional supplement contains abundant calcium elements, also contains microelements such as strontium, magnesium, aluminum, silicon, iron and the like, and also contains various amino acids such as aspartic acid, glycine, glutamic acid and the like, and can provide the microelements and the amino acid requirements for the microalgae; the chitin is mainly used as the shell of the shrimps and the crabs and can improve organic carbon and organic nitrogen for the microalgae; and the nutritional supplement can ensure the growth of microalgae bait while reducing the using amount of inorganic fertilizer, thereby reducing the probability of water eutrophication and water quality deterioration.
The following examples were obtained for each material:
obtaining shell powder: removing tissue from scallop collected from market, cleaning shell with clear water, oven drying at 100 deg.C, pulverizing with pulverizer, and sieving with 100 mesh sieve to obtain shell powder with particle size of 100 mesh.
Obtaining chitin powder: chitin is purchased from Qingdao cloud biotech ltd and has a molecular weight of 150 kDa; the chitin raw material is refined by a grinder and is sieved by a 100-mesh sieve, and the chitin powder with the particle size of 100 meshes is obtained.
Obtaining artificial zeolite powder: the artificial zeolite particles are purchased from Shanghai Michelin Biochemical technology, Inc., and are manually crushed, refined by a crusher, and sieved by a 100-mesh sieve to obtain the artificial zeolite powder with the particle size of 100 meshes.
Example 1:
the composition is prepared from the following raw materials in percentage by mass: 100g of shell powder (76.9%), 20g of chitin powder (15.4%), 6g of artificial zeolite powder (4.6%), 3g of alum (2.3%) and 1g of potassium hydrogen persulfate (0.7%).
Example 2:
the composition is prepared from the following raw materials in percentage by mass: 100g of shell powder (86.9%), 10g of chitin powder (8.7%), 3g of artificial zeolite powder (2.6%), 1.5g of alum (1.3%) and 0.5g of potassium hydrogen persulfate (0.4%).
Example 3:
the composition is prepared from the following raw materials in percentage by mass: 100g of shell powder (68%), 30g of chitin (20.4%), 9g of artificial zeolite powder (6.1%), 6g of alum (4.1%) and 2g of potassium hydrogen persulfate (1.4%).
Example 4:
the composition is prepared from the following raw materials in percentage by mass: 200g of shell powder (86.9%), 20g of chitin powder (8.7%), 6g of artificial zeolite powder (2.6%), 3g of alum (1.3%) and 1g of potassium hydrogen persulfate (0.4%).
Example 5:
the composition is prepared from the following raw materials in percentage by mass: shell powder 200g (81.0%), chitin powder 30g (12.1%), artificial zeolite powder 9g (3.6%), alum 6g (2.4%), potassium hydrogen persulfate 2g (0.8%). The nutrient obtained by the embodiment is used for verifying whether the nutrient regulates the pH value of the water body and promotes the growth of microalgae bait, and the following experiments are carried out:
the supplement of example 2 was added to artificial seawater at 5% and 10% respectively, and the change in pH of the water before and after the addition was examined for 18 days.
The supplement of example 2 was added to 100mL of sterilized seawater at 1g (1 wt%) and 2g (2 wt%) of each nutrient, and then 1mL of nutrient salt (containing 6mg of sodium nitrate, 0.4mg of potassium dihydrogen phosphate, 1.8mg of urea, 0.05mg of ferric citrate) was added, and after mixing, 1mL of Platymonas grandis (Platymonas Helgolandica tsingtaoensis) at logarithmic growth phase was added, and cultured in light at 20 ℃ for 12 h/dark for 12h, and the content of photosynthetic pigments in the microalgae was measured after 1 month, as follows: taking 2mL of culture solution in a 10mL centrifuge tube, centrifuging for 5min at 12000rpm, removing supernatant, adding 2mL of 80% acetone solution for suspension, centrifuging for 5min at 12000rpm in a water bath at 55 ℃, taking supernatant in a 10mL centrifuge tube, diluting to 5mL with 80% acetone, and measuring absorbance at 440nm, 663nm and 645nm (see FIG. 2), wherein the measurement formulas of chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoid (Car) are as follows:
Chl a=12.7ⅹOD663-2.69ⅹOD645
Chl b=22.9ⅹOD663-4.68ⅹOD645
Car=4.7ⅹOD663–0.27ⅹ(a+b)。
as can be seen from fig. 1, the addition of the nutritional supplement results in a significant increase in carbonate content in the water, with the result that the pH rises significantly and then stabilizes at an alkaline level, whereby acidification of the water is avoided. As can be seen from FIG. 2, the light and pigment contents of the Zostera marina, Qingdao, can be significantly increased after the nutritional supplement is added, i.e., the growth of the microalgae is significantly promoted.
Therefore, the nutritional supplement can improve and stabilize the pH of the water body, improve the saturation of carbonate and calcium carbonate in the water body, improve the abundance of microalgae bait and promote the growth of aquatic animals taking microalgae as the bait on the premise of reducing the application of fertilizers.

Claims (8)

1. An aquaculture nutritional supplement, comprising: the nutrition replenisher comprises, by weight, 70-90 parts of shell powder, 8-20 parts of chitin powder, 2-6 parts of artificial zeolite powder, 1-4 parts of alum and 0.3-1.0 part of potassium hydrogen persulfate.
2. The aquaculture nutritional supplement of claim 1, wherein: the above-mentioned
The shell powder is prepared from shell shells or snail shells through cleaning, high-temperature drying, crushing and sieving (100-200 meshes); wherein the shellfish shell is one or more of mussel, scallop, clam, oyster and razor clam, and the snail shell is one or more of whelk and conch.
3. The aquaculture nutritional supplement of claim 1, wherein: the chitin powder is prepared by crushing chitin with molecular weight of 20-200kDa to particle size of less than 100 meshes; wherein the chitin is chitin and/or its downstream product chitosan.
4. The aquaculture nutritional supplement of claim 1, wherein: the artificial zeolite powder is prepared by crushing artificial zeolite to 60-200 meshes for later use.
5. An aquaculture nutritional supplement according to any one of claims 1-4 wherein: the nutritional supplement is prepared into powder, granules or pills.
6. A method of preparing an aquaculture nutritional supplement of claim 1, wherein: the aquaculture nutritional supplement is prepared by mixing the ingredients as described in claim 1.
7. Use of an aquaculture nutritional supplement according to claim 1, characterized in that: the supplement is applied to the bait additive in the breeding culture of marine aquaculture crops; the dosage of it is 2-6kg/m2
8. Use of an aquaculture nutritional supplement according to claim 7 wherein: the aquaculture crops are shellfish, snails, shrimps, crabs and phytophagous fishes; the bait is fresh water or marine microalgae.
CN202210008704.8A 2022-01-06 2022-01-06 Aquaculture nutritional supplement and preparation method thereof Pending CN114343085A (en)

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Application Number Priority Date Filing Date Title
CN202210008704.8A CN114343085A (en) 2022-01-06 2022-01-06 Aquaculture nutritional supplement and preparation method thereof

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CN114343085A true CN114343085A (en) 2022-04-15

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101756063A (en) * 2008-11-04 2010-06-30 洪福山 Feed additive capable of improving growth environment of cultured fish and shrimp
CN104364317A (en) * 2012-04-13 2015-02-18 流体固体股份公司 Biodegradable material made of biological components
CN107950816A (en) * 2017-12-20 2018-04-24 威海市四合生物科技有限公司 A kind of microelement-supplementing product and its preparation process applied to aquaculture

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101756063A (en) * 2008-11-04 2010-06-30 洪福山 Feed additive capable of improving growth environment of cultured fish and shrimp
CN104364317A (en) * 2012-04-13 2015-02-18 流体固体股份公司 Biodegradable material made of biological components
CN107950816A (en) * 2017-12-20 2018-04-24 威海市四合生物科技有限公司 A kind of microelement-supplementing product and its preparation process applied to aquaculture

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