CN114324698A - Liquid chromatograph pump flow calibrating device - Google Patents
Liquid chromatograph pump flow calibrating device Download PDFInfo
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- CN114324698A CN114324698A CN202111398857.XA CN202111398857A CN114324698A CN 114324698 A CN114324698 A CN 114324698A CN 202111398857 A CN202111398857 A CN 202111398857A CN 114324698 A CN114324698 A CN 114324698A
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- connecting plate
- control display
- density
- liquid chromatograph
- pump flow
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- 239000007788 liquid Substances 0.000 title claims abstract description 23
- 238000005303 weighing Methods 0.000 claims abstract description 18
- 238000001514 detection method Methods 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 2
- 229930195733 hydrocarbon Natural products 0.000 description 2
- 150000002430 hydrocarbons Chemical class 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 238000004566 IR spectroscopy Methods 0.000 description 1
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- 238000004891 communication Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000003891 environmental analysis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
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- 239000007787 solid Substances 0.000 description 1
- 238000002336 sorption--desorption measurement Methods 0.000 description 1
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- 238000012795 verification Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention discloses a liquid chromatograph pump flow calibration device, which comprises a case body and a connecting plate, wherein a weighing balance is arranged in the case body, a container is arranged at the top of the weighing balance, an interface is arranged at the top of the container and fixedly connected with the case body, a calculator body is arranged in the case body and electrically connected with the weighing balance, and a printer body is arranged on one side of the case body, the liquid chromatograph pump flow calibration device has the beneficial effects that: through having added the interface, realized the unity of flow calibration, through having added the balance of weighing, realized the weight detection in the container, through having added the calculator body, realized the calculator body to the unified calculation of acquisition time, mobile phase density, collection number of times, through having added the printer body, realized that the printer body prints the structure that the computer body calculated, through having added the display screen that carries out, realized the numerical value display to the unified calculation of acquisition time, mobile phase density, collection number of times.
Description
Technical Field
The invention relates to the technical field of flow calibration, in particular to a pump flow calibration device of a liquid chromatograph.
Background
The liquid chromatograph uses the difference of the distribution ratio of the mixture between two liquids which are liquid-solid or immiscible, to separate the mixture first, and then analyze and identify the mixture. The system consists of a liquid storage device, a pump, a sample injector, a chromatographic column, a detector, a recorder and the like. The mobile phase in the liquid storage device is pumped into the system by a high pressure pump, the sample solution enters the mobile phase through the sample injector and is loaded into a chromatographic column (stationary phase) by the mobile phase, and because each component in the sample solution has different distribution coefficients in two phases, when the two phases move relatively, through the distribution process of repeated adsorption-desorption, each component generates a large difference in moving speed, is separated into single components and flows out of the column in sequence, when the sample passes through the detector, the concentration of the sample is converted into an electric signal and is transmitted to a recorder, and data is printed out in a map form by the high performance liquid chromatograph mainly comprising a sample injection system, a liquid infusion system, a separation system, a detection system and a data processing system. Since HPLC has the advantages of high resolution, high sensitivity, high speed, repeated use of a chromatographic column, easy collection of an effluent component, and the like, HPLC is widely used in various fields such as biochemistry, food and drug analysis, medical research, environmental analysis, inorganic analysis, and the like. The combination of high performance liquid chromatography and structural instruments is an important development direction. Liquid chromatography-mass spectrometry techniques are generally regarded as important, such as analysis of carbamate pesticides and polynuclear aromatics; the liquid chromatography-infrared spectroscopy combined use is also developed rapidly, such as the analysis and determination of hydrocarbons in water and nonvolatile hydrocarbons in seawater in environmental pollution analysis, so that the environmental pollution analysis is developed newly. The measurement supervision and inspection of the instruments are enhanced, and the method has important significance on environmental protection and product quality safety.
The error of the set value of the pump flow is proposed as a necessary item in the verification procedure of JJG 705-. In the process, the calibration is generally carried out by adopting a weighing method, namely, the volumetric flask is filled with mobile phase pure methanol, and the volumetric flask is weighed before and after the mobile phase pure methanol is weighed for calculation. In this process, the following conditions may cause errors, which may result in inaccurate calibration values:
1. methanol has extremely high volatility;
2. the sealing type of the volumetric flask is poor;
3. the time is controlled artificially, and three times of average inaccuracy is realized;
4. human error is introduced in the weighing process.
Disclosure of Invention
The present invention aims to provide a pump flow calibration device for a liquid chromatograph, so as to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a liquid chromatograph pump flow calibration device comprises a case body and a connecting plate, wherein a weighing balance is arranged inside the case body, a container is arranged at the top of the weighing balance, an interface is arranged at the top of the container and fixedly connected with the case body, a calculator body is arranged inside the case body and electrically connected with the calculator body, a printer body is arranged on one side of the case body, the connecting plate is arranged on one side of the case body, a case display is arranged on one side of the connecting plate, a density control display is arranged on one side of the connecting plate, a time control display is arranged on one side of the connecting plate and below the density control display, a frequency control display is arranged on one side of the connecting plate and below the time control display, a density control component matched with the density control display is arranged on one side of the connecting plate, one side of the connecting plate is provided with a time control assembly matched with the time control display, one side of the connecting plate is provided with a frequency control assembly matched with the frequency control display, and the density control display, the time control display and the frequency control display are all electrically connected with the calculator body.
As a preferred embodiment of the present invention: the density control subassembly includes that density promotes the button, one side of connecting plate is equipped with and promotes the button with density control display complex density, one side of connecting plate is established and is had density with density control display complex and reduce the button.
As a preferred embodiment of the present invention: the time control assembly comprises a time lifting button, one side of the connecting plate is provided with the time lifting button matched with the time control display, and one side of the connecting plate is provided with a time reducing button matched with the time control display.
As a preferred embodiment of the present invention: the frequency control assembly comprises a frequency lifting button, one side of the connecting plate is provided with a frequency lifting button matched with the frequency control display, and one side of the connecting plate is provided with a frequency reducing button matched with the frequency control display.
As a preferred embodiment of the present invention: and a calculator switch button matched with the calculator body is arranged on one side of the connecting plate.
As a preferred embodiment of the present invention: one side of the connecting plate is provided with a printer switch button matched with the printer body.
As a preferred embodiment of the present invention: the calculator body is electrically connected with the printer body.
As a preferred embodiment of the present invention: the case display is electrically connected with the calculator body.
Compared with the prior art, the invention has the beneficial effects that: the invention has simple structure, novelty and strong practicability, realizes the unification of flow calibration by adding the interface, realizes the weight detection in the container by adding the weighing balance, realizes the unified calculation of the calculator body on the acquisition time, the density of the mobile phase and the acquisition times by adding the calculator body, realizes the printing of the structure calculated by the printer body on the computer body by adding the printer body, realizes the adjustment of the density of the mobile phase by adding the density control component, realizes the adjustment of the acquisition time by adding the time control component, realizes the adjustment of the acquisition times by adding the time control component, and realizes the numerical display of the unified calculation of the acquisition time, the density of the mobile phase and the acquisition times by adding the display screen.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic view of the internal structure of the main body of the case according to the present invention;
FIG. 3 is a schematic view of a connection plate according to the present invention;
FIG. 4 is a schematic view of the internal connection structure of the present invention.
In the figure: 1. a chassis body; 2. a weighing balance; 3. an interface; 4. a container; 5. a calculator body; 6. a printer body; 7. a density control display; 8. a time control display; 9. a frequency control display; 10. a calculator switch button; 11. a printer switch button; 12. a case display; 13. a density control assembly; 131. a density boost button; 132. a density reduction button; 14. a time control component; 141. a time boost button; 142. a time reduction button; 15. a number control component; 151. a number of times increasing button; 152. a number reduction button; 16. a connecting plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: a liquid chromatograph pump flow calibration device comprises a case body 1 and a connecting plate 16, wherein a weighing balance 2 is arranged inside the case body 1, a container 4 is arranged at the top of the weighing balance 2, an interface 3 is arranged at the top of the container 4, the interface 3 is fixedly connected with the case body 1, a calculator body 5 is arranged inside the case body 1, the weighing balance 2 is electrically connected with the calculator body 5, a printer body 6 is arranged at one side of the case body 1, the connecting plate 16 is arranged at one side of the case body 1, a case display 12 is arranged at one side of the connecting plate 16, a density control display 7 is arranged at one side of the connecting plate 16, a time control display 8 is arranged at one side of the connecting plate 16 and below the density control display 7, a frequency control display 9 is arranged at one side of the connecting plate 16 and below the time control display 8, a density control component 13 matched with the density control display 7 is arranged at one side of the connecting plate 16, one side of connecting plate 16 is equipped with time control assembly 14 with time control display 8 complex, and one side of connecting plate 16 is equipped with number of times control assembly 15 with number of times control display 9 complex, and density control display 7, time control display 8 and number of times control display 9 all are connected with calculator body 5 electric connection, and the balance 2 of being convenient for weighs in to container 4, has improved calibration efficiency.
The density control assembly 13 comprises a density increasing button 131, one side of the connecting plate 16 is provided with a density increasing button 131 matched with the density control display 7, one side of the connecting plate 16 is provided with a density reducing button 132 matched with the density control display 7 to facilitate the density of the mobile phase to be increased or reduced by the density control assembly 13, the calibration efficiency is improved, the time control assembly 14 comprises a time increasing button 141, one side of the connecting plate 16 is provided with a time increasing button 141 matched with the time control display 8, one side of the connecting plate 16 is provided with a time reducing button 142 matched with the time control display 8 to facilitate the numerical adjustment of the acquisition time by the time control assembly 14, the calibration efficiency is improved, the number control assembly 15 comprises a number increasing button 151, one side of the connecting plate 16 is provided with a number increasing button 151 matched with the number control display 9, one side of the connecting plate 16 is provided with a number reducing button 152 matched with the number control display 9, convenient to time control assembly 15 adjusts the collection number of times, the efficiency of calculation has been improved, one side of connecting plate 16 is equipped with calculator shift knob 10 with calculator body 5 complex, it controls calculator body 5 to be convenient for calculator shift knob 10, the work efficiency of device has been improved, one side of connecting plate 16 is equipped with printer shift knob 11 with printer body 6 complex, it controls printer body 6 to be convenient for printer shift knob 11, the work efficiency of printer body 6 has been improved, calculator body 5 and printer body 6 electric connection, it prints the numerical value that calculator body 5 calculated to be convenient for printer body 6, the accuracy of calibrating device has been improved, machine case display 12 and calculator body 5 electric connection, it shows calculator body 5's numerical value to be convenient for machine case display 12, the work efficiency of calibration has been improved.
Specifically, when the portable mobile phase sampler is used, a specific container 4 is provided with an interface 3 and is used for containing a mobile phase of a chromatograph, the weighing balance 2 is used for weighing, the result is transmitted to a calculator body 5, the calculator body 5 is opened through a calculator switch button 10, the acquisition time is adjusted through a time control component 14, the density of the mobile phase is adjusted through a density control component 13, the acquisition frequency is adjusted through a frequency control component 15, the uniform calculation is carried out, when the acquisition frequency is 3, the density of the mobile phase is 0.791g/m, the acquisition time is 5min, the mass is 4.9852g, the mobile phase is displayed on a case display 12, the printer body 6 is opened through a printer switch button 11, and the result is printed out in the configured printer body 6.
In the description of the present invention, it is to be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "other end", "upper", "one side", "top", "inner", "front", "center", "both ends", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
Furthermore, the terms "first", "second", "third", "fourth" are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated, whereby the features defined as "first", "second", "third", "fourth" may explicitly or implicitly include at least one such feature.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "disposed," "connected," "secured," "screwed" and the like are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate, and may be communication between two elements or interaction relationship between two elements, unless otherwise specifically limited, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to specific situations.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides a liquid chromatograph pump flow calibrating device, includes quick-witted case body (1) and connecting plate (16), its characterized in that, the inside of quick-witted case body (1) is equipped with weighing balance (2), the top of weighing balance (2) is equipped with container (4), the top of container (4) is equipped with interface (3), interface (3) and quick-witted case body (1) fixed connection, the inside of quick-witted case body (1) is equipped with calculator body (5), weighing balance (2) and calculator body (5) electric connection, one side of quick-witted case body (1) is equipped with printer body (6), one side of quick-witted case body (1) is equipped with connecting plate (16), one side of connecting plate (16) is equipped with quick-witted case display (12), one side of connecting plate (16) is equipped with density control display (7), one side of connecting plate (16) and the below that is located density control display (7) are equipped with time control Display (8), one side of connecting plate (16) and the below that is located time control display (8) are equipped with number of times control display (9), one side of connecting plate (16) is equipped with density control subassembly (13) with density control display (7) complex, one side of connecting plate (16) is equipped with time control subassembly (14) with time control display (8) complex, one side of connecting plate (16) is equipped with number of times control subassembly (15) with number of times control display (9) complex, density control display (7), time control display (8) and number of times control display (9) all with calculator body (5) electric connection.
2. The pump flow calibration apparatus for liquid chromatograph of claim 1, wherein: the density control assembly (13) comprises a density lifting button (131), one side of the connecting plate (16) is provided with the density lifting button (131) matched with the density control display (7), and one side of the connecting plate (16) is provided with a density reducing button (132) matched with the density control display (7).
3. The pump flow calibration apparatus for liquid chromatograph of claim 1, wherein: the time control assembly (14) comprises a time lifting button (141), a time lifting button (141) matched with the time control display (8) is arranged on one side of the connecting plate (16), and a time reducing button (142) matched with the time control display (8) is arranged on one side of the connecting plate (16).
4. The pump flow calibration apparatus for liquid chromatograph of claim 1, wherein: the frequency control assembly (15) comprises a frequency lifting button (151), a frequency lifting button (151) matched with the frequency control display (9) is arranged on one side of the connecting plate (16), and a frequency reducing button (152) matched with the frequency control display (9) is arranged on one side of the connecting plate (16).
5. The pump flow calibration apparatus for liquid chromatograph of claim 1, wherein: and a calculator switch button (10) matched with the calculator body (5) is arranged on one side of the connecting plate (16).
6. The pump flow calibration apparatus for liquid chromatograph of claim 1, wherein: and a printer switch button (11) matched with the printer body (6) is arranged on one side of the connecting plate (16).
7. The pump flow calibration apparatus for liquid chromatograph of claim 1, wherein: the calculator body (5) is electrically connected with the printer body (6).
8. The pump flow calibration apparatus for liquid chromatograph of claim 1, wherein: the case display (12) is electrically connected with the calculator body (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111398857.XA CN114324698A (en) | 2021-11-19 | 2021-11-19 | Liquid chromatograph pump flow calibrating device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111398857.XA CN114324698A (en) | 2021-11-19 | 2021-11-19 | Liquid chromatograph pump flow calibrating device |
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| CN114324698A true CN114324698A (en) | 2022-04-12 |
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| CN202111398857.XA Pending CN114324698A (en) | 2021-11-19 | 2021-11-19 | Liquid chromatograph pump flow calibrating device |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6058764A (en) * | 1997-08-28 | 2000-05-09 | Hitachi, Ltd. | Analytical apparatus, liquid chromatography analyzer and a method therefor |
| US20080142444A1 (en) * | 2006-12-01 | 2008-06-19 | Hitachi High-Technologies Corporation | Liquid chromatograph apparatus |
| CN202421149U (en) * | 2012-01-09 | 2012-09-05 | 山东创新药物研发有限公司 | Conveniently adjusted solution conveyor used for high performance liquid chromatography |
| CN104678015A (en) * | 2015-02-11 | 2015-06-03 | 王峰 | Liquid chromatograph |
| CN112415121A (en) * | 2020-11-20 | 2021-02-26 | 上海仪电分析仪器有限公司 | Automatic calibration device for constant flow pump of liquid chromatograph and working method |
| CN112903853A (en) * | 2021-01-22 | 2021-06-04 | 武汉赛尔夫科技有限公司 | Control system and control method of high performance liquid chromatograph |
-
2021
- 2021-11-19 CN CN202111398857.XA patent/CN114324698A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6058764A (en) * | 1997-08-28 | 2000-05-09 | Hitachi, Ltd. | Analytical apparatus, liquid chromatography analyzer and a method therefor |
| US20080142444A1 (en) * | 2006-12-01 | 2008-06-19 | Hitachi High-Technologies Corporation | Liquid chromatograph apparatus |
| CN202421149U (en) * | 2012-01-09 | 2012-09-05 | 山东创新药物研发有限公司 | Conveniently adjusted solution conveyor used for high performance liquid chromatography |
| CN104678015A (en) * | 2015-02-11 | 2015-06-03 | 王峰 | Liquid chromatograph |
| CN112415121A (en) * | 2020-11-20 | 2021-02-26 | 上海仪电分析仪器有限公司 | Automatic calibration device for constant flow pump of liquid chromatograph and working method |
| CN112903853A (en) * | 2021-01-22 | 2021-06-04 | 武汉赛尔夫科技有限公司 | Control system and control method of high performance liquid chromatograph |
Non-Patent Citations (3)
| Title |
|---|
| 吴晓惠;黄彬磊;吴成新;蒲玲;朱佳奇;: "液相色谱仪电雾式检测器的校准", 化学分析计量, no. 02, 20 March 2019 (2019-03-20) * |
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